CN1048403C - Photosensitizer of traditional herb medicines and its production technology - Google Patents
Photosensitizer of traditional herb medicines and its production technology Download PDFInfo
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- CN1048403C CN1048403C CN93103432A CN93103432A CN1048403C CN 1048403 C CN1048403 C CN 1048403C CN 93103432 A CN93103432 A CN 93103432A CN 93103432 A CN93103432 A CN 93103432A CN 1048403 C CN1048403 C CN 1048403C
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- photosensitizer
- ethanol
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Abstract
The present invention relates to a traditional Chinese medicine photosensitizer and preparation method thereof, particularly to a traditional Chinese medicine photosensitizer for photosensitization therapy and preparation technology thereof. The present invention is characterized in that the photosensitizer contains chlorine 6 and 60 to 80 % of polymer; silkworm droppings are used as raw material; the preparation technology comprises the steps that the raw material and acetone are mixed to be extracted; then NaOH is added to the mixture to be saponified by ethanol; then magnesium is removed by acidification; the mixture is mixed and stirred with silica gel G; then the mixture is eluted, and impurities are removed respectively by petroleum ether and ethanol; then the mixture is dissolved by distilled water and vaporized to prepare the finished product. The prepared finished product is black brown loose powder, is easy to be dissolved into water, is not dissolved into an organic solvent and is sensitive to light. The photosensitizer has the advantages of good treatment effect, convenient clinical application, simple preparation technology, easy operation, and low cost and easy acquirement of the raw material.
Description
The present invention relates to be used for the Chinese medicine photosensitizer and the production technology of photosensitization therapy.
Photosensitization therapy is a kind of quick, the new technique of non-invasive diagnosis and treatment cancer, the external in recent years tumor bound pair step is attached great importance to, and obtained faster and made progress, but the research of photosensitizer and selection are the important steps of this photosensitization therapy always, the photosensitizer that uses clinically both at home and abroad is hematoporphyrin derivative (HPD) at present, hemoporphyrin is taken from animal blood, the retention time is long in the body, the photosensitive side effect of skin is strong, take 30 day time lucifuge, therefore make troubles to extensive use diagnosis and treatment, seek a kind of new, the curative effect height, drain fast, the photosensitizer that toxicity is low, significant.
The objective of the invention is to overcome the defective of above-mentioned existence, make every effort to use the Bombyx mori L. feedstock production that is easy to get that a kind of Chinese medicine photosensitizer is provided, and provide the production technology of this photosensitizer.
Described photosensitizer is characterized in that containing chlorin e (CHLORINe6) and polymer 60-80% thereof, is raw material with the Bombyx mori L., through acetone separation extract crude extract, then through saponification, acidify, remove impurity, refining obtaining.
Above-mentioned Chinese medicine photosensitizer is characterized in that containing chlorin e (CHLORINe6) and polymer 60-65% thereof.
Above-mentioned Chinese medicine photosensitizer is characterized in that containing the xanthein 20-40% based on carotin.
The production technology of above-mentioned Chinese medicine photosensitizer is a raw material with the Bombyx mori L., and presses following processing step:
1) gets Bombyx mori L. and mix, under 90-100 ℃ of temperature reflux 1-3 hour, filter waste and also reclaim acetone with the acetone that 3-7 doubly measures.
2) the above-mentioned crude extract that obtains is mixed with 95% ethanol that 1-5 doubly measures, heating for dissolving under agitation is incorporated as crude extract 1-3 10%NaOH doubly then, and under 90-100 ℃ of temperature reflux 12-20 hour, and to keep pH value be 9-10.
3) after above-mentioned mixed liquor reclaims ethanol, dilution, static, remove the thick thing in upper strata, filter waste.
4) above-mentioned solution neutralization and adjusting pH value are 4-6, and be static, abandons supernatant, and precipitate is repeated to be washed to neutrality, and filtration is drained.
5) impurity removal and purification.
The production technology of above-mentioned a kind of Chinese medicine photosensitizer is characterized in that by following technological parameter:
A) above-mentioned 1) Bombyx mori L. is doubly measured acetone with 4-6 and is mixed reflux 1.5-2.5 hour.
B) above-mentioned 2) crude extract that obtains and 2-4 doubly measure 95% ethanol and mix, and 10%NaOH is that the 1.5-2.5 of crude extract doubly measures, reflux 15-17 hour.
The production technology of above-mentioned a kind of Chinese medicine photosensitizer is characterized in that described impurity removal and purification, mixes and stirs absorption with silica gel G earlier, with the petroleum ether eluting, volatilize the back and take out granum, reuse 95% ethanol elution again, and recovery ethanol, be dissolved in water at last and evaporate anhydrate refining finished product.
Described Chinese medicine photosensitizer is dark brown loose powder, and is soluble in water, is insoluble to organic solvent, sees photaesthesia, keeps in Dark Place.It is good that fruit is treated in these photosensitizer diagnosis and treatment, and clinical practice is convenient, can alleviate the side effect of skin photosensitizer, and toxicity is low, drains fast.And production technology is simple, and raw material is cheap and easy to get, has application value widely.
Below provide specific embodiment of the present invention, simultaneously the present invention is further described.
(1) thick chlorophyllous extraction
Get the Bombyx mori L. pulverize, cross the distiller sieve No. 20, take by weighing 200g and place the 2000ml round-bottomed flask, add 5 times of amount acetone, add condenser reflux in water bath with thermostatic control, each two hours, about four times, filter, reclaim acetone, promptly get thick chlorophyll 10g (containing chlorophyll 10-15%).
(2) phytyl is gone in saponification
Get thick chlorophyll 200g, add 95% ethanol triplication, heating in water bath 20 minutes, the about 400ml of dissolving back agitation and dropping 10%NaOH, the adjusting pH value is 9-10, and heating in water bath refluxed about 16 hours, surveyed PH at any time, after reclaiming ethanol, add 20 times of about 4000ml of amount of distilled water, at a static night, remove the thick thing in upper strata, filter, filtrate keeps.
(3) magnesium is removed in acidify
Getting that above-mentioned solution adds 10%HCl neutralization and regulate pH value is 5, and at a static night, precipitate is on the bottom, and supernatant inclines to discard and repeats to be washed to neutrality then, and filtration is drained.
(4) impurity removal and purification
Get the silica gel that above-mentioned solid adds about one times of amount, mix thoroughly, be loose shape, with petroleum ether eluting (200g uses petroleum ether 6000ml approximately), till not having xanthein to eluent, volatilize the back and take out granum, place round-bottomed flask to add the about triplication of 95% ethanol, transferring PH is 9, same operation eluting 6 times, merge eluent, filtered and recycled ethanol gets the about 5g of chlorin e.Add about 10 times of water gagings dissolving at last, the water-bath evaporation is anhydrated, and must make with extra care finished product, and test dissolubility 100mg/ml should clarify, and survey content must be measured and be Bombyx mori L. 0.1-0.08%.
Claims (3)
1, a kind of production technology that contains the Chinese medicine photosensitizer of chlorin e and polymer thereof is characterized in that with the Bombyx mori L. being raw material, and presses following processing step:
1) get Bombyx mori L. and mix with the acetone that 3-7 doubly measures, under 90-100 ℃ of temperature reflux 1-3 hour, filter waste and also reclaim acetone,
2) the above-mentioned crude extract that obtains is mixed with 95% ethanol that 1-5 doubly measures, heating for dissolving under agitation is incorporated as crude extract 1-3 10%NaOH doubly then, and under 90-100 ℃ of temperature reflux 12-20 hour, and to keep pH value be 9-10,
3) after above-mentioned mixed liquor reclaimed ethanol, dilution, static, the thick thing in removal upper strata filtered waste,
4) above-mentioned solution neutralization and adjusting pH value are 4-6, and be static, abandons supernatant, and precipitate is repeated to be washed to neutrality, and filtration is drained,
5) impurity removal and purification.
2, the production technology of a kind of Chinese medicine photosensitizer as claimed in claim 1 is characterized in that by following technological parameter:
A) above-mentioned 1) Bombyx mori L. is doubly measured acetone with 4-6 and is mixed, and reflux 1.5-2.5 hour,
B) above-mentioned 2) crude extract that obtains and 2-4 doubly measure 95% ethanol and mix, and 10%NaOH is that the 1.5-2.5 of crude extract doubly measures, reflux 15-17 hour.
3, the production technology of a kind of Chinese medicine photosensitizer as claimed in claim 1, it is characterized in that described impurity removal and purification, earlier mix and stir absorption with silica gel G, again with the petroleum ether eluting, volatilize the back and take out granum, reuse 95% second jealous woman eluting, and reclaim ethanol, be dissolved in water at last and evaporate anhydrate refining finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93103432A CN1048403C (en) | 1993-03-26 | 1993-03-26 | Photosensitizer of traditional herb medicines and its production technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93103432A CN1048403C (en) | 1993-03-26 | 1993-03-26 | Photosensitizer of traditional herb medicines and its production technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1092981A CN1092981A (en) | 1994-10-05 |
| CN1048403C true CN1048403C (en) | 2000-01-19 |
Family
ID=4984655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN93103432A Expired - Lifetime CN1048403C (en) | 1993-03-26 | 1993-03-26 | Photosensitizer of traditional herb medicines and its production technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1048403C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100387240C (en) * | 2003-06-16 | 2008-05-14 | 北京中亚深蓝生物技术开发有限公司 | An extract of faeces bombycis and the preparation method thereof, use and device for diagnosing or treating malignant tumours |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102936423B (en) * | 2012-10-25 | 2014-07-02 | 常州美胜生物材料有限公司 | Brown pigment, and preparation method and application thereof |
| GB2558809B (en) | 2013-01-09 | 2018-11-07 | International Moisture Analysers Ltd | Optical chemical analyser |
-
1993
- 1993-03-26 CN CN93103432A patent/CN1048403C/en not_active Expired - Lifetime
Non-Patent Citations (1)
| Title |
|---|
| 中国药学杂志 1993.1.8 钟济秀等，蚕少光敏剂的皮肤光毒反应及毒性研究 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100387240C (en) * | 2003-06-16 | 2008-05-14 | 北京中亚深蓝生物技术开发有限公司 | An extract of faeces bombycis and the preparation method thereof, use and device for diagnosing or treating malignant tumours |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1092981A (en) | 1994-10-05 |
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Owner name: ELLEN BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: INST. OF TRADITIONAL CHINESE MEDICINE, ZHEJIANG PROV. Effective date: 20040806 |
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Effective date of registration: 20040806 Address after: Queensland, Australia Patentee after: Ireland biotech Co Address before: 310007 No. 26 Tianmu Mountain Road, Zhejiang, Hangzhou Patentee before: Zhejiang Traditional Chinese Medicine Research Institute |
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Owner name: DALIAN AORUI SCIENCE CO., LTD. Free format text: FORMER OWNER: ELLEN BIOTECHNOLOGY CO., LTD. Effective date: 20050304 |
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Effective date of registration: 20050304 Address after: 116023 No. 3, Gaoxin street, hi tech park, Liaoning, Dalian Patentee after: Dalian auretech Technology Co. Ltd. Address before: Queensland, Australia Patentee before: Ireland biotech Co |
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| CX01 | Expiry of patent term |
Expiration termination date: 20130326 Granted publication date: 20000119 |