CN104833669B - A kind of quick determination method of weak main ingredient signal medicine - Google Patents
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Abstract
The present invention provides a kind of quick determination method of weak main ingredient signal medicine, comprises the following steps：The determination of weak main ingredient signal medicine；Sample solution and its standard solution, the preparation of analogue standard solution；The in situ detection of the spot of thin-layer sample application；The calculating of the Rf value of thin-layer developing.The quick determination method of the weak main ingredient signal medicine of the present invention can realize that medicine that is weak to main ingredient signal and having analogue is used for quickly detecting, so as to judge authenticity of medicament and quality.
Description
Technical field
The present invention relates to a kind of quick determination method of weak main ingredient signal medicine, belong to drug measurement techniques field.
Background technology
Raman spectroscopy is a kind of medicine Fast Detection Technique emerging in recent years, has the advantage such as easy, quick.Typically
Commercially available chemicals drug content is higher, main ingredient spectral signal is stronger, the raman spectral signal of adjuvant is not enough to cover
The characteristic raman spectral signal of main ingredient, and main ingredient composition does not have analogue, therefore be easy to by portable Raman
Spectrometer is used for quickly detecting, so as to judge the true and false of the medicine and quality.
But some main ingredient signal is weak and has the medicine quick detection of analogue highly difficult, often by illegal business
Dealer uses, manufacture and sell pass a fake product off as a genuine one, shoddy counterfeit drug.
This similar drug is because drug content is low, and auxiliary material content is of a relatively high, therefore main ingredient is substantially buried in auxiliary material.
When irradiating this similar drug by Raman spectrometer laser, it is difficult to be irradiated to main ingredient position.Or due to main ingredient Raman scattering compared with
It is weak, and the raman spectral signal of adjuvant is relatively strong, masks the characteristic raman spectral signal of main ingredient substantially.So as to medicine
The rapid field detection band of the true and false is come difficult.
Due also to the structure class that chemical constitution is similar, chemical property is similar, Raman spectral information is similar be present in this similar drug
Like thing, although the analogue of these low price can also play therapeutic effect, side effect is big.Due to depositing for analogue
So that this similar drug can not determine main ingredient composition by Raman signal, cannot also be realized by Portable Raman spectrometer
To the Site Detection of the similar drug.
For this kind of main ingredient signal weak (hereinafter referred to as weak main ingredient signal medicine) and with analogue medicine inspection
Survey, can only be realized at present by chromatographic technique, application of gas chromatorgraphy/mass technology, spectral imaging technology etc., but above-mentioned technology instrument it is huge and
Costliness, complex operation, analysis time length, be not suitable for the fast inspection in scene of medicine.
Therefore, a kind of new method of exploitation is needed badly at present, it is weak for field quick detection main ingredient signal and similar with structure
The medicine of thing.
The content of the invention
The present invention be in order to it is solving the above problems and carry out, and it is an object of the present invention to provide one kind can realize it is weak to main ingredient signal
And the medicine for having analogue is used for quickly detecting, so as to judge the method for authenticity of medicament and quality.
The present invention to achieve these goals, employs following technical scheme：
The present invention provides a kind of quick determination method of weak main ingredient signal medicine, for identifying weak main ingredient signal medicine
Main ingredient composition, it is characterised in that comprise the following steps：
Step 1, the determination of weak main ingredient signal medicine
Medicine to be checked and its standard items are detected using Portable Raman spectrometer, laser power be 150mw~
300mw, irradiation time are 10s~15s, respectively obtain respective Raman spectrogram, calculate the similarity of the two, if the phase of the two
It is less than 0.5 like degree, then can determine that the medicine to be checked is weak main ingredient signal medicine.
The preparation of step 2, sample solution and its standard solution, analogue standard solution
Weak main ingredient signal medicine is ground successively, dissolved, is ultrasonically treated, is then centrifuged for taking supernatant, is obtained certain
The sample solution of concentration, after the standard items dissolving corresponding with main ingredient composition, being ultrasonically treated, obtain and sample solution isoconcentration
Standard solution, after corresponding with main ingredient composition analogue standard items dissolving, being ultrasonically treated, obtain molten with sample
In the analogue standard solution of liquid isoconcentration, sample solution and standard solution, analogue standard solution
Solvent is identical, and the solvent does not dissolve the adjunct ingredient in weak main ingredient signal medicine；
Step 3, the in situ detection of the spot of thin-layer sample application
By sample solution and standard solution difference point sample on lamellae, obtaining two spots, then by Nano Silver peptization
Drop is added on two spots, is carried out in-situ scanning to two spots using Portable Raman spectrometer, is respectively obtained respective
SERS figure and the similarity for calculating the two, if the similarity of the two (preferably 0.9-1) between 0.7-1,
Judge main ingredient composition for standard items composition or standard items analogue composition；
Step 4, the calculating of the Rf value of thin-layer developing
Sample solution, standard solution, analogue standard solution are distinguished into point sample on lamellae, utilize expansion
Agent is deployed respectively, and taking-up volatilizes, and is placed under uviol lamp and is inspected, it is determined that position of the spot on lamellae and being obtained after expansion
Rf value, the Rf value of comparative sample spot and other spots, if sample point is equal with the Rf value of standard items spot, sentences
Main ingredient composition in the fixed weak main ingredient signal medicine is consistent with the composition of standard items；If unequal, comparative sample spot and knot
The Rf value of structure analog spot, if sample point is equal with the Rf value of analogue spot, judge the weak main ingredient letter
Main ingredient composition in number medicine is consistent with the composition of its analogue.
Further, in a kind of quick determination method of weak main ingredient signal medicine of the present invention, can also have so
Feature：Wherein, described Step 1: in three, Raman spectra pretreatment method includes spectral coverage and chooses (400cm-1-1800cm-1), baseline correction (airPLS methods), smooth (Sgolay methods), similarity calculating method is the Corrcoef functions in Matlab.
Further, in a kind of quick determination method of weak main ingredient signal medicine of the present invention, can also have so
Feature：Wherein, in the step 2, the time of the supersound process is 20-30min.
Further, in a kind of quick determination method of weak main ingredient signal medicine of the present invention, can also have so
Feature：Wherein, in step 3, the laser power when Portable Raman spectrometer is scanned for 150mw~
300mw, irradiation time are 10s~15s.
Further, in a kind of quick determination method of weak main ingredient signal medicine of the present invention, can also have so
Feature：Wherein, Step 3: in four, the dropwise addition of the sample solution, standard solution and analogue standard solution
Measure as 1-2 μ L, the dripping quantity of the nano-silver colloid solution is 5-10 μ L.
Further, in a kind of quick determination method of weak main ingredient signal medicine of the present invention, can also have so
Feature：Wherein, described Step 3: in four, the lamellae is thin layer silica gel plate.
Further, in a kind of quick determination method of weak main ingredient signal medicine of the present invention, can also have so
Feature：Wherein, in the step 4, the wavelength of the uviol lamp is 254nm.
Further, in a kind of quick determination method of weak main ingredient signal medicine of the present invention, can also have so
Feature：Wherein, the excitation wavelength 785nm of the Portable Raman spectrometer.
Invention effect and effect
According to a kind of quick determination method of weak main ingredient signal medicine of the present invention, because weak main ingredient signal medicine is made
Sample solution and standard solution point sample in obtaining spot on lamellae, by nano-silver colloid solution well prepared in advance be added dropwise in
On spot, spot is detected using Portable Raman spectrometer, respective SERS figure can be obtained, and
By comparing the similarity of collection of illustrative plates between them, whether the main component that can determine that out the medicine is active ingredient, reaches judgement
The purpose of authenticity of medicament；Further, sample solution, standard solution and analogue standard solution are subjected to thin layer exhibition
Open, main ingredient composition analogue whether consistent with standard items or for standard items is judged according to Rf value, reaches judgement
The purpose of drug quality quality；, can be at detection scene and the nano-silver colloid solution and lamellae prepared is all easy to carry about with one
Coordinate with portable Raman spectrometer, it is easy to realize to weak main ingredient signal authenticity of medicament and the quick detection of quality.
Brief description of the drawings
Fig. 1 is the SERS (SERS) of present invention main ingredient signal medicine weak in embodiment one and standard items
Figure；
Fig. 2 is the present invention weak in embodiment one main ingredient signal medicine, standard items and analogue standard items through thin layer
Schematic diagram after expansion；
Fig. 3 is the SERS (SERS) of present invention main ingredient signal medicine weak in embodiment two and standard items
Figure；And
Fig. 4 is the flow chart of the present invention.
Embodiment
A kind of quick determination method of weak main ingredient signal medicine involved in the present invention is elaborated below.
<Embodiment one>
In the present embodiment one, by taking terazosin as an example, the weak main ingredient signal medicine of specific explanations quick detection
Method.
The quick determination method of the weak main ingredient signal medicine of the present embodiment one comprises the following steps,
Step 1, the determination of weak main ingredient signal medicine
Medicine to be checked and its standard items are detected using Portable Raman spectrometer, laser power 150mw, irradiated
Time is 10s, respectively obtains respective Raman spectrogram.
In the present embodiment, the model BWS415-785H (Bi Da Imtech of the U.S.) of Portable Raman spectrometer, swash
Send out wavelength 785nm.
(400cm is chosen by spectral coverage-1-1800cm-1), baseline correction, smoothly two Raman spectrums are pre-processed
Afterwards, the similarity of two above-mentioned Raman spectrograms is calculated using the Corrcoef functions in Matlab, as a result finds the two phase
It is -0.0525 like degree, less than 0.5, then can determine that the medicine to be checked is weak main ingredient signal medicine, namely the Raman signal of auxiliary material is covered
The Raman signal of main ingredient composition is covered.
Step 2, the preparation of nano-silver colloid solution
Precision weighs 17mg silver nitrate, 8.5mgPVP (polyvinylpyrrolidone), is dissolved in 5mL water, and obtaining mass ratio is
2:1 AgNO3/ PVP solution.50mLDMF (DMF) is measured into 250mL three-necked bottle, is heated to seething with excitement.
It is rapidly added above-mentioned AgNO3Simultaneously constantly boiling for a period of time, lucifuge guarantor in brown bottle is placed in after being cooled to room temperature to/PVP solution
Deposit.
The preparation of step 3, sample solution and its standard solution, analogue standard solution
A piece of terazosin is taken, the quality of the Terazosin Hydrochloride in the terazosin is 2mg.
After the terazosin is ground, dissolved in 1mL methanol, after supersound process 20min, centrifuging and taking supernatant
Liquid, produce sample solution.Methanol can not dissolve the adjunct ingredient in terazosin so that after solution is centrifuged, auxiliary material
It is removed into branch by forming precipitation, therefore there was only Terazosin Hydrochloride in sample solution as solute.
The standard items of accurately weighed 2.0mg Terazosin Hydrochloride, standard items are dissolved in 1mL methanol, at ultrasound
After managing 20min, 2mg/mL Terazosin Hydrochloride standard solution is produced.
The standard items of accurately weighed 2.0mg minipress, standard items are dissolved in 1mL methanol, are ultrasonically treated
After 20min, 2mg/mL minipress standard solution is produced.
Step 4, the in situ detection of the spot of thin-layer sample application
Pipette sample solution and each 1 μ L of Terazosin Hydrochloride standard solution respectively with capillary, point sample is in silica gel respectively
On lamellae, two spots are obtained, then 5 μ L nano-silver colloid solution is added dropwise on two spots, utilize portable Raman light
Spectrometer carries out in-situ scanning to two spots, respectively obtains respective SERS figure, specific as shown in Figure 1.
(400cm is chosen by spectral coverage-1-1800cm-1), baseline correction, smoothly two Raman spectrums are pre-processed
Afterwards, the similarity of two above-mentioned Raman spectrograms is calculated using the Corrcoef functions in Matlab, draws sample solution
Be 0.7920 with Terazosin Hydrochloride standard solution similarity, between 0.7-1, then judge main ingredient composition and standard items or
The composition of analogue standard items cannot be distinguished by, namely the main ingredient composition in the terazosin is Terazosin Hydrochloride
Or minipress, it need to determine whether.
Step 5, the calculating of the Rf value of thin-layer developing
It is in situ in lamellae to pipette sample solution and each 1 μ L point samples of standard solution respectively with capillary, with chloroform：
N-butanol：Mixed solvent (the chloroform of glacial acetic acid：N-butanol：The volume ratio of glacial acetic acid is 4：3：0.5) deploy for solvent
Afterwards, taking-up volatilizes, and position of the spot on lamellae after expansion is inspected under uviol lamp 254nm, and calculate Rf value.Wherein,
Rf value be in thin-layered chromatography origin to spot centers distance and origin to the distance of solvent front ratio.
From figure 2 it can be seen that Terazosin Hydrochloride sample solution Rf value is 0.575, and Terazosin Hydrochloride standard
Product solution Rf value is 0.225, and both Rf values are unequal.Illustrate the main ingredient in the terazosin in the present embodiment
The composition of composition and Terazosin Hydrochloride standard items is inconsistent.
Minipress is the analogue of Terazosin Hydrochloride.Prazosin Hydrochloride Tablets side effect is very big, and valency
Lattice therefore are usually utilized to pretend to be terazosin to carry out market pin than the cheap half of terazosin
Sell.
Then, the standard solution of minipress is deployed by step identical in step 5, and by Rf value
Rf value after deploying with sample solution is contrasted, and as a result finds that the Rf value that minipress standard items obtain is 0.575,
It is identical with the Rf value of sample solution.Therefore the main ingredient composition and salt in the terazosin in the present embodiment be can be determined that
The composition of sour prazosin standard items is consistent, shows that the terazosin in the present embodiment is present and is emitted with less expensive configuration analog
Situation about filling.
Embodiment acts on and effect
The quick determination method of a kind of weak main ingredient signal medicine according to involved by the present embodiment, because hydrochloric acid spy is drawn into azoles
Point sample is in obtaining spot respectively on lamellae for sample solution, standard solution made of piperazine, by nano-silver colloid well prepared in advance
Solution is added dropwise on spot, and spot is detected using Portable Raman spectrometer, can be obtained respective surface enhanced and be drawn
Graceful spectrogram, and by comparing the similarity of collection of illustrative plates between them, can determine that out the medicine main component whether be effectively into
Point, reach the purpose for judging authenticity of medicament；Further, by the standard items of sample solution, standard solution and analogue
Solution carries out thin-layer developing, judges whether main ingredient composition consistent with standard items or structure class for standard items according to Rf value
Like thing, reach the purpose for judging drug quality quality；And the nano-silver colloid solution and lamellae prepared is all easy to carry about with one, energy
It is enough to detect the cooperation of live and portable Raman spectrometer, it is easy to realize to the terazosin true and false and quality
Quick detection.
<Embodiment two>
In the present embodiment two, by taking glimepiride tablet as an example, the method for the weak main ingredient signal medicine of specific explanations quick detection.
The quick determination method of the weak main ingredient signal medicine of the present embodiment two comprises the following steps,
Step 1, the determination of weak main ingredient signal medicine
Medicine to be checked and its standard items are detected using Portable Raman spectrometer, laser power 300mw, irradiated
Time is 15s, respectively obtains respective Raman spectrogram.
In the present embodiment, the model BWS415-785H (Bi Da Imtech of the U.S.) of Portable Raman spectrometer, swash
Send out wavelength 785nm.
(400cm is chosen by spectral coverage-1-1800cm-1), baseline correction, smoothly two Raman spectrums are pre-processed
Afterwards, the similarity of two above-mentioned Raman spectrograms is calculated using the Corrcoef functions in Matlab, as a result finds the two phase
It is 0.1200 like degree, less than 0.5, then can determine that the medicine to be checked is weak main ingredient signal medicine, namely the Raman signal of auxiliary material is covered
The Raman signal of main ingredient composition is covered.
Step 2, the preparation of nano-silver colloid solution
Precision weighs 17mg silver nitrate, 8.5mgPVP, is dissolved in 5mL water, obtains mass ratio as 2:1 AgNO3/ PVP is molten
Liquid.In the three-necked bottle for measuring 50mLDMF to 250mL, it is heated to seething with excitement.It is rapidly added above-mentioned AgNO3/ PVP solution simultaneously continues
Boiling a period of time, it is cooled to after room temperature to be placed in brown bottle and is kept in dark place.
The preparation of step 3, sample solution and its standard solution
A piece of glimepiride tablet is taken, the quality of the Glimepiride in the glimepiride tablet is 2mg.
It is 1 in 1mL methanol and the volume ratio of chloroform after the glimepiride tablet is ground:1 in the mixed solvent dissolving,
After being ultrasonically treated 30min, centrifuging and taking supernatant, 2mg/mL sample solution is produced.Mixed solvent can not dissolve glimepiride tablet
In adjunct ingredient so that after solution is centrifuged, adjunct ingredient can be removed by forming precipitation, thus in sample solution only
Glimepiride is as solute.
The standard items of accurately weighed 2.0mg Glimepiride, by standard items 1mL methanol and chloroform volume ratio be 1:
1 in the mixed solvent dissolving, after being ultrasonically treated 30min, produce 2mg/mL standard solution.
Step 4, the in situ detection of the spot of thin-layer sample application
Pipette sample solution and each 2 μ L of standard solution respectively with capillary, point sample obtains on silica gel thin-layer plate respectively
Two spots, then 10 μ L nano-silver colloid solution is added dropwise on two spots, using Portable Raman spectrometer to two spots
Point carries out in-situ scanning, respectively obtains respective SERS figure, specific as shown in Figure 3.
(400cm is chosen by spectral coverage-1-1800cm-1), baseline correction, smoothly two Raman spectrums are pre-processed
Afterwards, the similarity of above-mentioned two Raman spectrograms is calculated using the Corrcoef functions in Matlab, draw sample solution with
The Similarity value of Glimepiride standard items is 0.9660, between 0.7-1 and more than 0.9, then judges main ingredient composition and standard
The composition of product is consistent, namely the main ingredient composition in the glimepiride tablet is Glimepiride.
Embodiment acts on and effect
The quick determination method of a kind of weak main ingredient signal medicine according to involved by the present embodiment, because by Glimepiride system
Into sample solution and standard solution point sample is in obtaining spot respectively on lamellae, by nano-silver colloid solution well prepared in advance
It is added dropwise on spot, spot is detected using Portable Raman spectrometer, respective surface-enhanced Raman light can be obtained
Spectrogram, and by comparing the similarity of collection of illustrative plates between them, whether the main component that can determine that out the medicine is active ingredient, is reached
To the purpose for judging authenticity of medicament；, can be existing in detection and the nano-silver colloid solution and lamellae prepared is all easy to carry about with one
Field coordinates with portable Raman spectrometer, it is easy to realizes the quick detection to the glimepiride tablet true and false.
Certainly, a kind of quick determination method of weak main ingredient signal medicine of the present invention is not merely defined in above reality
Apply the description in example.
Claims (8)
1. a kind of quick determination method of weak main ingredient signal medicine, for identifying the main ingredient composition of weak main ingredient signal medicine, its
It is characterised by, comprises the following steps：
Step 1, the determination of weak main ingredient signal medicine
Medicine to be checked and its standard items are detected using Portable Raman spectrometer, laser power is 150mw ~ 300mw, is shone
It is 10s ~ 15s to penetrate the time, respectively obtains respective Raman spectrogram, calculates the similarity of the two, if the similarity of the two is less than
0.5, then can determine that the medicine to be checked is weak main ingredient signal medicine；
The preparation of step 2, sample solution and its standard solution, analogue standard solution
Weak main ingredient signal medicine is ground successively, dissolved, is ultrasonically treated, is then centrifuged for taking supernatant, obtains finite concentration
Sample solution, after corresponding with main ingredient composition standard items dissolving, being ultrasonically treated, obtain the mark with sample solution isoconcentration
Quasi- product solution, after corresponding with main ingredient composition analogue standard items dissolving, being ultrasonically treated, obtain with sample solution etc.
The analogue standard solution of concentration, sample solution and the solvent in standard solution, analogue standard solution
Identical, the solvent does not dissolve the adjunct ingredient in weak main ingredient signal medicine；
Step 3, the in situ detection of the spot of thin-layer sample application
Sample solution and standard solution difference point sample are dripped on lamellae, obtaining two spots, then by nano-silver colloid solution
It is added on two spots, in-situ scanning is carried out to two spots using Portable Raman spectrometer, respectively obtains respective surface
Enhancing Raman spectrogram simultaneously calculates the similarity of the two, if the similarity of the two between 0.7-1, judges main ingredient composition for mark
The composition of the composition of quasi- product or the analogue of standard items；
Step 4, the calculating of the Rf value of thin-layer developing
By sample solution, standard solution, analogue standard solution difference point sample on lamellae, solvent point is utilized
Do not deployed, taking-up volatilizes, and is placed under uviol lamp and inspects, it is determined that position of the spot on lamellae and being obtained after expansion than moving
Value, the Rf value of comparative sample spot and other spots, if sample point is equal with the Rf value of standard items spot, judging should
Main ingredient composition in weak main ingredient signal medicine is consistent with the composition of standard items；If unequal, comparative sample spot and structure class
Like the Rf value of thing spot, if sample point is equal with the Rf value of analogue spot, the weak main ingredient signal medicine is judged
Main ingredient composition in product is consistent with the composition of its analogue.
A kind of 2. quick determination method of weak main ingredient signal medicine according to claim 1, it is characterised in that：
Wherein, described Step 1: in three, Raman spectra pretreatment method includes choosing 400cm-1-1800 cm-1Spectral coverage, use
AirPLS methods carry out baseline correction, carried out smoothly with Sgolay methods, and similarity calculating method is the Corrcoef letters in Matlab
Number.
A kind of 3. quick determination method of weak main ingredient signal medicine according to claim 1, it is characterised in that：
Wherein, in the step 2, the time of the supersound process is 20-30min.
A kind of 4. quick determination method of weak main ingredient signal medicine according to claim 1, it is characterised in that：
Wherein, in step 3, the laser power when Portable Raman spectrometer is scanned is 150mw ~ 300mw, is shone
It is 10s ~ 15s to penetrate the time.
A kind of 5. quick determination method of weak main ingredient signal medicine according to claim 1, it is characterised in that：
Wherein, Step 3: in four, the dripping quantity of the sample solution, standard solution and analogue standard solution
For 1-2 μ L, the dripping quantity of the nano-silver colloid solution is 5-10 μ L.
A kind of 6. quick determination method of weak main ingredient signal medicine according to claim 1, it is characterised in that：
Wherein, described Step 3: in four, the lamellae is thin layer silica gel plate.
A kind of 7. quick determination method of weak main ingredient signal medicine according to claim 1, it is characterised in that：
Wherein, in the step 4, the wavelength of the uviol lamp is 254nm.
A kind of 8. quick determination method of weak main ingredient signal medicine according to claim 1, it is characterised in that：
Wherein, the excitation wavelength 785nm of the Portable Raman spectrometer.
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| CN105223182A (en) * | 2015-09-01 | 2016-01-06 | 中国人民解放军第二军医大学 | A kind of method detecting doped chemical drugs in Chinese patent drug |
| CN107505305B (en) * | 2017-07-26 | 2020-09-04 | 中国人民解放军第二军医大学 | Method for rapidly analyzing illegal addition of sildenafil analogue in yang-strengthening health-care product |
| CN107727634B (en) * | 2017-09-26 | 2021-02-09 | 上海化工研究院有限公司 | Laser Raman spectrum resolution processing method |
| CN108802001B (en) * | 2018-05-02 | 2020-09-04 | 中国人民解放军第二军医大学 | Quick detection method for weak main medicine signal medicine |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103926130A (en) * | 2014-05-06 | 2014-07-16 | 山东师范大学 | Method for extracting biotin with molasses as raw material and detection method |
| CN103954608A (en) * | 2014-05-20 | 2014-07-30 | 中国人民解放军第二军医大学 | Method for identifying whether blood-glucose-lowering traditional Chinese medicine contains pioglitazone or rosiglitazone or not |
| CN103968946A (en) * | 2014-05-19 | 2014-08-06 | 中国人民解放军第二军医大学 | Acquisition method of surface enhanced Raman two-dimensional correlation spectrum |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI507672B (en) * | 2013-05-29 | 2015-11-11 | Univ Nat Yang Ming | Method for manufacturing and using a test paper and chemical composition thereof |
-
2015
- 2015-04-29 CN CN201510213240.4A patent/CN104833669B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103926130A (en) * | 2014-05-06 | 2014-07-16 | 山东师范大学 | Method for extracting biotin with molasses as raw material and detection method |
| CN103968946A (en) * | 2014-05-19 | 2014-08-06 | 中国人民解放军第二军医大学 | Acquisition method of surface enhanced Raman two-dimensional correlation spectrum |
| CN103954608A (en) * | 2014-05-20 | 2014-07-30 | 中国人民解放军第二军医大学 | Method for identifying whether blood-glucose-lowering traditional Chinese medicine contains pioglitazone or rosiglitazone or not |
Non-Patent Citations (4)
| Title |
|---|
| "Analysis of low active-pharmaceutical-ingredient signal drugs based on thin layer chromatography and surface-enhanced Raman spectroscopy";Xiao Li 等;《Journal of Pharmaceutical and Biomedical Analysis》;20160914;第131卷;第410-419页 * |
| "TLC_SERS法快速检测降压类中药中非法添加的四种化学成分";朱青霞 等;《光谱学与光谱分析》;20140430;第34卷(第4期);第990-992页第1-2节 * |
| "基于拉曼光谱法的药用辅料掺假快检方法研究";高群 等;《第十七届全国分子光谱学学术会议论文集》;20130828;第144页2.2节 * |
| "拉曼光谱法在假药快检中的研究进展";柳艳 等;《药学实践杂志》;20121125;第30卷(第6期);第401-404、446页 * |
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