CN104830816B - A kind of flavonoids prenyltransferase AhFDT2 and its encoding gene and application - Google Patents
A kind of flavonoids prenyltransferase AhFDT2 and its encoding gene and application Download PDFInfo
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Abstract
The invention discloses a kind of flavonoids prenyltransferase AhFDT2 and its encoding gene and application.Present invention clone from jackfruit obtains flavonoids isopentenyl transferase genes AhFDT2, and its nucleotide sequence is as shown in SEQ ID NO.1；The flavonoids prenyltransferase AhFDT2 of coding, its amino acid sequence is as shown in SEQ ID NO.2.By using flavonoids isopentenyl transferase genes AhFDT2 construction of expression vector transformed saccharomyces cerevisiaes, acquisition overexpression flavonoids prenyltransferase AhFDT2 saccharomyces cerevisiae；Using its bacterium solution or microsome catalysis isopentene group donor and flavonoids substrate synthesis isopentene group flavonoids, conversion ratio may be up to 26%, and product purity is up to 76 95%.The present invention provides a kind of new method for the biosynthesis of isopentene group flavonoids, has easy to operate, small, the advantage such as product purity height of reaction pollution.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of flavonoids prenyltransferase AhFDT2 and its coding
Gene and application.
Background technology
Jackfruit (Artocarpus heterophyllus Lam.) belongs to Moraceae fruit plant, mainly plants in heat
Band, subtropical area.Fruit aromatic flavour, sweet and sour taste are deep to be favored by south China consumer.Jackfruit is not only one kind
Characteristic fruit resource, its branches and leaves tissue are rich in the Flavonoid substances of a kind of special construction, i.e. isopentene group flavonoids.This kind of flavones
Class material is more stronger than the bioactivity of flavones substance of common structure, such as it is suppressing proliferative activity o f tumor, immune tune
Activity etc. is saved, the Flavonoid substances than no isopentene group are more notable.Isopentene group flavonoids in jackfruit tissue
Content it is not only high, and structure species enrich, and have now been found that the iso-amylene base class of more than 20 kind different structure forms is yellow
Ketone.As can be seen here, jackfruit is both abundant isopentene group flavonoids natural resources, and flavonoids prenyltransferase
Affluent resources.Isopentene group flavonoids has very important value in terms of the disease treatments such as tumour, has a wide range of applications
Prospect.Therefore, using the intrinsic inherent advantage of jackfruit, a kind of biosynthesis iso-amylene is developed by the means of biotechnology
The method of base class flavones is by with important industrial value.
The content of the invention
First purpose of the present invention is to provide a kind of flavonoids prenyltransferase AhFDT2 and its encoding gene
AhFDT2。
The flavonoids prenyltransferase AhFDT2 of the present invention, its amino acid sequence is as shown in SEQ ID NO.2.
Present invention also offers the flavonoids isopentene group transfer for encoding above-mentioned flavonoids prenyltransferase AhFDT2
Enzyme gene AhFDT2, its nucleotide sequence is as shown in SEQ ID NO.1.
Second object of the present invention is to provide flavonoids prenyltransferase AhFDT2 and is preparing iso-amylene base class Huang
Application in ketone.
Described application is preferably to add apiolin into overexpression flavonoids prenyltransferase AhFDT2 wine brewing
In yeast liquid, it is catalyzed through flavonoids prenyltransferase AhFDT2 and produces isopentene group apiolin.
Described application is preferably using flavonoids and isopentene group donor as substrate, through flavonoids prenyltransferase
AhFDT2 catalysis produces isopentene group flavonoids.
It is preferred that described catalysis, the pH value of its reaction system is 7~9, and reaction temperature is 20~40 DEG C.
It is preferred that described flavonoids is apiolin, described isopentene group donor is dimethyl propylene alkenyl pyrophosphoric acid, through class
Flavones prenyltransferase AhFDT2 catalysis produces isopentene group apiolin.
It is preferred that described flavonoids is Kaempferol, described isopentene group donor is dimethyl propylene alkenyl pyrophosphoric acid, through class
Flavones prenyltransferase AhFDT2 catalysis produces isopentene group Kaempferol.
Present invention clone from jackfruit obtains flavonoids isopentenyl transferase genes AhFDT2, its flavonoids encoded
Prenyltransferase AhFDT2 has the good activity for catalyzing and synthesizing isopentene group flavonoids.By using flavonoids isoamyl
Alkenyl transferase gene AhFDT2 construction of expression vector transformed saccharomyces cerevisiaes, obtain overexpression flavonoids prenyltransferase
AhFDT2 saccharomyces cerevisiae；Utilize its bacterium solution or microsome catalysis isopentene group donor and flavonoids substrate synthesis iso-amylene base class
Flavones, conversion ratio may be up to 26%, and product purity is up to 76-95%.The present invention carries for the biosynthesis of isopentene group flavonoids
A kind of new method has been supplied, there is easy to operate, small, the advantage such as product purity height of reaction pollution.
Brief description of the drawings
Fig. 1 is the first mass spectrometric figure of isopentene group apiolin.
Fig. 2 is the second order mses figure of isopentene group apiolin.
Fig. 3 is the bacterium colony PCR primer electrophoretogram of saccharomyces cerevisiae positive bacteria.
Fig. 4 is the first mass spectrometric figure of isopentene group Kaempferol.
Fig. 5 is the second order mses figure of isopentene group Kaempferol.
Embodiment
Following examples are to further explanation of the invention, rather than limitation of the present invention.
The experimental method not indicated specifically in following Examples, can conventionally be carried out.
Embodiment 1：Clone flavonoids isopentenyl transferase genes AhFDT2, structure overexpression vector and conversion wine brewing ferment
It is female
The RNA of jackfruit blade is extracted, using reverse transcriptase M-MLV, the cDNA of reverse transcription reaction synthesis.Using the cDNA as
Template, using forward primer GCCACCATGGAGCTCTCAATTTCT, reverse primer
TATGAATGGAAATAAGAAAAATTCTGCA, TakaRa company Ex Taq archaeal dna polymerases, enter performing PCR amplification；PCR conditions
For：94 DEG C, 5min；94 DEG C, 45s, 55 DEG C, 1min, 72 DEG C, 1.5min；35 circulations；72 DEG C of extension 10min.Using agarose
Purpose fragment is reclaimed in gel electrophoresis, and purpose fragment is delivered into sequencing.Through sequencing analysis, the flavonoids isopentene group for cloning to obtain turns
Enzyme gene AhFDT2 is moved, for its nucleotide sequence as shown in SEQ ID NO.1, it contains 1209 bases, the albumen name of coding
For flavonoids prenyltransferase AhFDT2, totally 402 amino acid, specific amino acid sequence is as shown in SEQ ID NO.2.
Connection flavonoids isopentenyl transferase genes AhFDT2 to overexpression vector pYes2.1TOPO (is purchased from
Invitrogen companies, article No. K4150-01) on, coupled reaction condition is：Flavonoids isopentenyl transferase genes AhFDT2,
Plasmid pYES2.1TOPO, 1.2M NaCl, 0.06M MgCl2After mixing, room temperature places 30min and completes coupled reaction, is weighed
(flavonoids isopentenyl transferase genes AhFDT2 is inserted into the restructuring matter after overexpression vector pYes2.1TOPO to group plasmid
Grain).Then it is converted into Saccharomyces cerevisiae competent cell again, conversion condition is：2 μ L are added into competent yeast cells
Recombinant plasmid, 5 μ L salmon sperm dnas, mix；Adding 600 μ L conversion fluids, (converting formula of liquid is：1mL 50%PEG, 125 μ L 10
× TE, 125 10 × LiAc of μ L), 30 DEG C, 100rpm shaking tables conversion 30min；Add 70 μ L DMSO (dimethyl sulfoxide (DMSO)), 42 DEG C
Heat shock 15min；Ice bath places 13000rpm centrifugation 5s after 2min, adds 200 μ L 1 × TE suspension cells, and coating SC-U suppresses flat
Plate, 30 DEG C are cultivated 3~5 days.Bacterium colony PCR screens recombinant plasmid successful conversion to the positive bacteria of brewing yeast cell, screening technique
For：Select single bacterium colony and be transferred to 50 μ L sterilized waters, 100 DEG C of heating 5min, centrifugation removes cell fragment；10 μ L of supernatant liquid are taken,
Add 5 μ L Ex Taq DNA polymerase buffers liquid, 1 μ L 10mM dNTPs, 1 μ L forward primers
GCCACCATGGAGCTCTCAATTTCT, 1 μ L reverse primer ACCGAGGAGAGGGTTAGGGAT, 0.5 μ L from plasmid sequence
Ex Taq archaeal dna polymerases, 31.5 μ L sterilized waters, mixing；PCR programs are：94 DEG C, 5min；94 DEG C, 40s；55 DEG C, 40s；72
DEG C, 1.5min；35 circulations；72 DEG C of extension 10min.PCR primer is detected with agarose gel electrophoresis, as a result as shown in figure 3, figure
3 M is marker, and 1 is positive bacteria, and the positive bacteria can amplify purpose fragment, shows flavonoids prenyltransferase base
The recombinant plasmid successful conversion after overexpression vector pYes2.1TOPO is inserted into because of AhFDT2 to brewing yeast cell, then through surveying
Sequence checking confirms, thus obtains flavonoids isopentenyl transferase genes AhFDT2 being inserted into overexpression vector
Recombinant plasmid successful conversion after pYes2.1TOPO is named as saccharomyces cerevisiae to the positive bacteria of brewing yeast cell
pYes2.1TOPO-AhFDT2.Mass propgation is carried out to saccharomyces cerevisiae pYes2.1TOPO-AhFDT2, chooses saccharomyces cerevisiae first
PYes2.1TOPO-AhFDT2 is transferred to 50mL SC-U and suppresses culture medium (2% glucose is carbon source), 30 DEG C, 200rpm, cultivates
To exponential phase；Cell is collected by centrifugation, is diluted to SC-U inducing cultures (2% galactolipin, 1% gossypose are carbon source)
OD600=0.4,30 DEG C, 200rpm, cultivate 24h.With SC-U inducing culture culture saccharomyces cerevisiaes pYes2.1TOPO-
AhFDT2, its overexpression flavonoids prenyltransferase AhFDT2 is induced, that is, obtain overexpression flavonoids isopentene group
Transferase AhFDT2 saccharomyces cerevisiae bacterium solution.
Embodiment 2：Synthesize flavonoids isopentenyl transferase genes AhFDT2, structure overexpression vector and conversion wine brewing ferment
It is female
Flavonoids isopentenyl transferase genes AhFDT2 full length sequences are directly synthesized (specifically such as using fully synthetic method
Shown in SEQ ID NO.1), flavonoids isopentenyl transferase genes AhFDT2 is connected to overexpression according to the method for embodiment 1
On carrier pYes2.1TOPO, then convert into Saccharomyces cerevisiae competent cell, obtain saccharomyces cerevisiae pYes2.1TOPO-
AhFDT2.Saccharomyces cerevisiae pYes2.1TOPO-AhFDT2 is subjected to mass propgation, induction overexpression flavonoids isopentene group turns
Enzyme AhFDT2 is moved, obtains overexpression flavonoids prenyltransferase AhFDT2 saccharomyces cerevisiae bacterium solution.
Embodiment 3：The biosynthesis of isopentene group apiolin
1. synthesize isopentene group apiolin
500 μ are added in the overexpression flavonoids prenyltransferase AhFDT2 of embodiment 1 saccharomyces cerevisiae bacterium solution
M apiolins, after 25 DEG C are reacted 48 hours, extracted using the ethanol solution of volume fraction 90%, ethanol mutually passes through C18 reversed-phase columns
(16mm × 460mm) is purified, with methanol：Water (50/50~100/0) elutes, and collects the elution that methanol/water volume ratio is 90/10
Component, obtain compound 1 (isopentene group apiolin).
2. the Structural Identification of compound 1 (isopentene group apiolin)
Compound 1 dissolves in methanol, and first mass spectrometric result is shown [M+H]+M/z=339.1, as shown in figure 1, showing the change
Adduct molecule amount is 338, than the molecular weight that apiolin has more an isopentene group.Second order mses generate two fragments, and m/z divides
Not Wei 283.1 and 165.1, as shown in Figure 2, it was demonstrated that the isopentene group is connected on the A rings of apiolin.Thereby confirm that compound 1
For isopentene group apiolin.
The conversion ratio of method synthesis isopentene group apiolin of the present embodiment is used as 19%, purity 83%.
Embodiment 4：The biosynthesis of isopentene group apiolin
500 μ are added in the overexpression flavonoids prenyltransferase AhFDT2 of embodiment 1 saccharomyces cerevisiae bacterium solution
M apiolins, 35 DEG C reaction 2 hours after, using the ethanol solution of volume fraction 60% extract, by C18 reversed-phase columns (16mm ×
460mm) purify, with methanol：Water (50/50~100/0) elutes, and collects the elution fraction that methanol/water volume ratio is 90/10, obtains
To isopentene group apiolin.Using the conversion ratio of method synthesis isopentene group apiolin of the present embodiment, purity is for 8%
80%.
Embodiment 5：The biosynthesis of isopentene group apiolin
500 μ are added in the overexpression flavonoids prenyltransferase AhFDT2 of embodiment 1 saccharomyces cerevisiae bacterium solution
M apiolins, 30 DEG C reaction 24 hours after, using the ethanol solution of volume fraction 80% extract, by C18 reversed-phase columns (16mm ×
460mm) purify, with methanol：Water (50/50~100/0) elutes, and collects the elution fraction that methanol/water volume ratio is 90/10, obtains
To isopentene group apiolin.Using the conversion ratio of method synthesis isopentene group apiolin of the present embodiment, purity is for 24%
76%.
Embodiment 6：The biosynthesis of isopentene group apiolin
It is collected by centrifugation in the overexpression flavonoids prenyltransferase AhFDT2 saccharomyces cerevisiae bacterium solution of embodiment 2
Bacterial strain, extract microsome；100 μ g microsomes are taken, add 100mM Tris-HCl (pH7.0), 10mM Mg2+、500μM DMAPP
(dimethyl propylene alkenyl pyrophosphoric acid), 500 μM of apiolins, after 20 DEG C are reacted 0.5 hour, using ethyl acetate extractive reaction liquid, second
Acetoacetic ester is mutually purified by C18 reversed-phase columns (16mm × 460mm), with methanol：Water (50/50~100/0) elutes, and collection methanol/
Water volume ratio is 90/10 elution fraction, obtains isopentene group apiolin (structure determination of the compound is with embodiment 3).Adopt
The conversion ratio that isopentene group apiolin is synthesized with the method for the present embodiment is 16%, purity 95%.
Embodiment 7：The biosynthesis of isopentene group apiolin
It is collected by centrifugation in the overexpression flavonoids prenyltransferase AhFDT2 saccharomyces cerevisiae bacterium solution of embodiment 1
Bacterial strain, extract microsome；100 μ g microsomes are taken, add 100mM Tris-HCl (pH9.0), 10mM Mg2+、500μM DMAPP
(dimethyl propylene alkenyl pyrophosphoric acid), 500 μM of apiolins, after 40 DEG C are reacted 24 hours, using ethyl acetate extractive reaction liquid, acetic acid
Ethyl ester is mutually purified by C18 reversed-phase columns (16mm × 460mm), with methanol：Water (50/50~100/0) elutes, and collects methanol/water
Volume ratio is 90/10 elution fraction, obtains isopentene group apiolin.Isopentene group celery is synthesized using the method for the present embodiment
The conversion ratio of element is 21%, purity 91%.
Embodiment 8：The biosynthesis of isopentene group apiolin
It is collected by centrifugation in the overexpression flavonoids prenyltransferase AhFDT2 saccharomyces cerevisiae bacterium solution of embodiment 1
Bacterial strain, extract microsome；100 μ g microsomes are taken, add 100mM Tris-HCl (pH8.0), 10mM Mg2+、500μM DMAPP
(dimethyl propylene alkenyl pyrophosphoric acid), 500 μM of apiolins, after 30 DEG C are reacted 24 hours, using ethyl acetate extractive reaction liquid, acetic acid
Ethyl ester is mutually purified by C18 reversed-phase columns (16mm × 460mm), with methanol：Water (50/50~100/0) elutes, and collects methanol/water
Volume ratio is 90/10 elution fraction, obtains isopentene group apiolin.Isopentene group celery is synthesized using the method for the present embodiment
The conversion ratio of element is 26%, purity 95%.
Embodiment 9：The biosynthesis of isopentene group Kaempferol
1. synthesize isopentene group Kaempferol
It is collected by centrifugation in the overexpression flavonoids prenyltransferase AhFDT2 saccharomyces cerevisiae bacterium solution of embodiment 1
Bacterial strain, extract microsome；100 μ g microsomes are taken, add 100mM Tris-HCl (pH8.0), 10mM Mg2+、500μM DMAPP
(dimethyl propylene alkenyl pyrophosphoric acid), 500 μM of Kaempferols, after 30 DEG C are reacted 24 hours, using ethyl acetate extractive reaction liquid, acetic acid
Ethyl ester is mutually purified by C18 reversed-phase columns (16mm × 460mm), with methanol：Water (50/50~100/0) elutes, and obtains compound 2
(isopentene group Kaempferol).
2. the Structural Identification of compound 2 (isopentene group Kaempferol)
Compound 2 dissolves in methanol, and first mass spectrometric result is shown [M+H]+M/z=355.1, as shown in figure 3, showing the change
The molecular weight of compound 2 is 354, than the molecular weight that Kaempferol has more an isopentene group.Second order mses generate a fragment, m/z
For 299.1, as shown in figure 4, showing due to Kaempferol fragment caused by the fracture of isopentene group.It is different to thereby determine that compound 2
Pentenyl Kaempferol.
The conversion ratio of method synthesis isopentene group Kaempferol of the present embodiment is used as 14%, purity 92%.
Claims (8)
- A kind of 1. flavonoids prenyltransferase AhFDT2, it is characterised in that its amino acid sequence such as SEQ ID NO.2 institutes Show.
- A kind of 2. flavonoids prenyltransferase for encoding the flavonoids prenyltransferase AhFDT2 described in claim 1 Gene A hFDT2, it is characterised in that its nucleotide sequence is as shown in SEQ ID NO.1.
- 3. applications of the flavonoids prenyltransferase AhFDT2 in isopentene group flavonoids is prepared described in claim 1.
- 4. application according to claim 3, it is characterised in that apiolin is added into expression flavonoids prenyltransferase In AhFDT2 saccharomyces cerevisiae bacterium solution, it is catalyzed through flavonoids prenyltransferase AhFDT2 and produces isopentene group apiolin.
- 5. application according to claim 3, it is characterised in that be that substrate, warp are used as using flavonoids and isopentene group donor Flavonoids prenyltransferase AhFDT2 catalysis produces isopentene group flavonoids.
- 6. application according to claim 5, it is characterised in that described catalysis, the pH value of its reaction system is 7~9, instead It is 20~40 DEG C to answer temperature.
- 7. application according to claim 5, it is characterised in that described flavonoids is apiolin, described isopentene group Donor is dimethyl propylene alkenyl pyrophosphoric acid, is catalyzed through flavonoids prenyltransferase AhFDT2 and produces isopentene group apiolin.
- 8. application according to claim 5, it is characterised in that described flavonoids is Kaempferol, described isopentene group Donor is dimethyl propylene alkenyl pyrophosphoric acid, is catalyzed through flavonoids prenyltransferase AhFDT2 and produces isopentene group Kaempferol.
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