CN104823711A - Method for reducing inoculating pollution rate of edible fungi factory - Google Patents

Method for reducing inoculating pollution rate of edible fungi factory Download PDF

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Publication number
CN104823711A
CN104823711A CN201510203294.2A CN201510203294A CN104823711A CN 104823711 A CN104823711 A CN 104823711A CN 201510203294 A CN201510203294 A CN 201510203294A CN 104823711 A CN104823711 A CN 104823711A
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China
Prior art keywords
inoculation
purification
pollution rate
inoculating
edible mushroom
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CN201510203294.2A
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Chinese (zh)
Inventor
李辉平
曲绍轩
林金盛
蒋宁
侯立娟
马林
宋金俤
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Jiangsu Academy of Agricultural Sciences
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Jiangsu Academy of Agricultural Sciences
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Priority to CN201510203294.2A priority Critical patent/CN104823711A/en
Publication of CN104823711A publication Critical patent/CN104823711A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a method for reducing inoculating pollution rate of an edible fungi factory. The method comprises the following steps of in a purified inoculating room, forming filtered and purified upward air flow on an operation interface, and enabling limbs of an inoculating person, an inoculating machine and a fungus bag or fungus bottle, which are conveyed at the upper part of the operation interface to all be in the purified air flow which flows from bottom to top. The method has the advantage that by arranging a 100 class purifier under an operation platform, and providing the upstream air flow through an air pipe, dirt and foreign fungi are prevented from entering a fungus bag or a fungus bottle, and the inoculating success rate of the existing clean workshop is increased to be more than 99.9%, so the pollution rate of the edible fungi factory or other clean workshops can be greatly reduced.

Description

A kind of method reducing edible mushroom factory inoculation pollution rate
Technical field
The invention belongs to edible fungus industrial cultivation field, particularly relate to a kind of method reducing edible mushroom factory inoculation pollution rate.
Background technology
Through development for many years, China's Edible Fungi has the cultivation of traditional peasant household to enter the factorial praluction stage, and level of industrial technology promotes greatly.The mode of the inoculating hood inoculation of original employing gradually replace by the clean transfer room of batch production.
Patent CN200710054939.6 discloses a kind of high efficient inoculating room, inoculation workman can be made to enter indoor and to complete inoculation work, make inoculation operation convenient and swift thus, just form streamline vaccination ways with cooperating each other, effectively enhance productivity, reduce the labour intensity of workman.
Patent CN200910045037.5 discloses a kind of edible fungi inoculation room, it comprises purify air room and the air return room that are separated by the partition curtain of band handle hole, the purify air top of room and air return room is air filter chamber, purify air room top install high efficiency particulate air filter, medium air filtration net is installed at the top of air return room, and purify air room and air return indoor are separately installed with illuminating lamp and ultraviolet sterilizing lamp.
Above-mentioned two patents embody the processing mode of present edible mushroom factory of China cleaning inoculation environment substantially.In addition, utilize high efficiency particulate air filter treatment and purification air, after forming the higher transfer room environment of cleanliness factor, the producer also can purified treatment above further booster shot operating table surface.Patent CN201010132504.0 discloses the 100-stage cleaning laminar flow inoculation under barotropic atmosphere in a kind of edible fungus industrial cultivation, and new wind, to enter top, workshop higher than the pressure of normal atmosphere (An), purifies the backward lower discharge of laminar flow hood high-efficiency filtering and purifying through hundred grades; Inoculation personnel, inoculating mechanism and the conveying of bacterium bottle are all in hundred grades of purification laminar flows from top to bottom; The malleation of hundred grades of purification laminar flows is from top to bottom at least higher than the gas pressure intensity 30Pa in cooling vehicle and passageway, bacteria workshop.Tentatively show round-the-clock throughout the year in good time can inoculation by production requirement, the problem of poisonous residual quantity after there will not be standardization to sterilize, success ratio of inoculation is stable up to 99.7%.
But, above-mentioned edible fungi inoculation system, " new wind, to enter top, workshop higher than the pressure of normal atmosphere (An), purifies the backward lower discharge of laminar flow hood high-efficiency filtering and purifying through hundred grades ", although compare conventional vaccination to greatly reduce pollution rate, but still there is inoculation pollution hidden trouble.Edible mushroom cultivating bag (bottle) to be seeded is placed on inoculation table top in the mode of opening upwards, and the complete front of new wind blows to opening composts or fertilisers of cultivating charge level, and the probability making charge level contact miscellaneous bacteria improves greatly.
Although hundred grades of purification laminar flow hood high efficiency particulate airs are declared to filter most dusts, bacterium and spore, but the filter screen of different manufacturers, different batches, the difference of service time, replacement frequency, filter effect be there are differences, once strainer filtering poor effect, miscellaneous bacteria directly will be blown to charge level, causes inoculation environment bacterial contamination rate to improve.
Operating personnel in transfer room, inoculation material etc. all can carry a certain amount of dust and miscellaneous bacteria enters transfer room, is another primary pollution source in transfer room.The air-flow that laminar flow hood blows downwards, can by the air driven of both sides above operating table surface, and form gaseous exchange in transfer room, dust and miscellaneous bacteria are driven by air-flow, directly blow into cultivation bag (bottle) opening, pollute.
Summary of the invention
For overcoming the deficiencies in the prior art, the object of the present invention is to provide a kind of airflow direction changing clean operating space in transfer room, airflow direction is cultivation bag (bottle) opening direction dorsad, even if make the chance of the pollution factor contact charge levels such as miscellaneous bacteria greatly reduce, thus reduce the method for the edible mushroom factory inoculation of the pollution probability of inoculation link.
For achieving the above object, the present invention is achieved by the following technical solutions.
A kind of method reducing edible mushroom factory inoculation pollution rate, indoor at purification inoculation, operation interface is formed the updraft through filtration, purification, makes to be in the inoculation personnel limbs on operation interface top, inoculating mechanism and bacterium bag or bottle conveying is all in purifying gas flow from bottom to top.Prevent dust and miscellaneous bacteria from entering bacterium bag or bottle.
Further below described operation interface, hundred grades of polishing filters are installed, hundred grades of purification laminar flows are upwards provided.
Further be provided with air vent on described operation interface surface.Purifying gas flow is upwards flowed operation interface is all parallel everywhere.
Further being provided with air cleaning member at described purification inoculation chamber carries out filtration, purification to transfer room integrated air.
Further described air cleaning member outlet connects ventilation duct, and ventilation duct is laid in purification inoculation room floor, and ventilation duct tube wall is provided with ventilating opening upwards, makes the air-flow after purification from bottom to top in purification inoculation chamber flowing, prevents indoor gas convection current.
Beneficial effect: compared with prior art, the invention has the advantages that: by providing hundred grades of polishing filters below operating desk, updraft is provided by airduct, prevent dust and miscellaneous bacteria from entering bacterium bag or bottle, existing clean room success ratio of inoculation can be made to bring up to more than 99.9%, greatly reduce the pollution rate of edible mushroom factory or the operation of other clean room.
Accompanying drawing explanation
The structural representation of Fig. 1 purification inoculation chamber of the present invention;
The structural representation of the purification inoculation chamber of Fig. 2 prior art;
1. clean transfer room, 2. operating desks, 3. bacterium bag or bottle, 4. air cleaning member, 5. air vent, 6. ventilating opening in figure, 7. hundred grades of polishing filter, 8. airduct, 9. air-flows.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail.
As shown in Figure 1: the present invention reduces the method for edible mushroom factory inoculation pollution rate, and the method is: in purification inoculation chamber 1, provides hundred grades of polishing filter 7(to meet GB/T 13554-2008 " efficient below operating desk 2 air filteringdevice " standard is to the requirement of high efficiency particle air filter), the air vent 5 be provided with by operation interface surface provides updraft 9; Utilize the high efficiency particulate air filter of air cleaning member 4 to carry out purification inoculation chamber 1 integrated air and carry out filtration, purification, air-flow 9 after purification is upwards flowed by the ventilating opening 6 be laid on ventilation duct 8 tube wall on ground, prevent indoor gas convection current, prevent dust and miscellaneous bacteria from entering cultivation bag (bottle) 3, make existing clean room success ratio of inoculation bring up to more than 99.9%, greatly reduce the pollution rate of edible mushroom factory or the operation of other clean room.
Operating desk comprises operating table surface, hundred grades of polishing filters 7, blower fans; Operating table surface has several air vents 5, and blower fan and hundred grades of polishing filters 7 are positioned at below operating table surface, and blower fan provides power by air pressurized, and air, after hundred grades of polishing filters 7 purify, forms the clean gas flow of rising in operating table surface by air vent 5.
The above-mentioned gas pressure intensity entering purification inoculation chamber is at least higher than the gas pressure intensity 30Pa of purification inoculation outdoor.
In the edible fungus industrial cultivation proving ground of inventor unit, implement the transfer room design of the program, utilize the method to carry out plate and detect and Xingbao mushroom bag cultivation inoculation operational applications.
Before practical operation, whole purification run area decontamination equipment first runs 30min, makes system enter stable state.Operator, through toilet's working specification training, can grasp GMP.
Plate detects with reference to the GB/T 18204.3-2013 public places sanitary method of inspection the 3rd part: the detection method of air microbe.Plate is opened deutostoma and is placed on upward on operating desk, and simulation bacterium bag environment upwards, it is 0 that repeated detection result all shows every ware clump count.
Xingbao mushroom bacterium bag, after 121 DEG C of steam sterilizings, after being cooled to 25 DEG C, being brought transfer room into by workman, operating desk is carried out inoculation operation in purification cooling chamber, and inoculation operation meets specification.After having inoculated, bacterium bag enters culturing room and cultivates.Inoculate after 3 days, sampling statistics charge level contaminated bacteria bag, calculate inoculation pollution rate.Repeatedly statistics shows, inoculation pollution rate is all lower than 0.1%.
The present invention is illustrated according to above-described embodiment, should be appreciated that above-described embodiment does not limit the present invention in any form, and all employings are equal to replacement or the technical scheme that obtains of equivalent transformation mode, all drop within protection scope of the present invention.

Claims (6)

1. one kind is reduced the method for edible mushroom factory inoculation pollution rate, it is characterized in that, indoor at purification inoculation, operation interface is formed the updraft through filtration, purification, makes to be in the inoculation personnel limbs on operation interface top, inoculating mechanism and bacterium bag or bottle conveying is all in purifying gas flow from bottom to top.
2. a kind of method reducing edible mushroom factory inoculation pollution rate according to claim 1, is characterized in that, below described operation interface, install hundred grades of polishing filters, provides hundred grades of purification laminar flows upwards.
3. a kind of method reducing edible mushroom factory inoculation pollution rate according to claim 1 and 2, is characterized in that, be provided with air vent on described operation interface surface.
4. a kind of method reducing edible mushroom factory inoculation pollution rate according to claim 1, is characterized in that, be provided with air cleaning member carry out filtration, purification to transfer room integrated air at described purification inoculation chamber.
5. a kind of method reducing edible mushroom factory inoculation pollution rate according to claim 4, it is characterized in that, described air cleaning member outlet connects ventilation duct, ventilation duct is laid in purification inoculation room floor, ventilation duct tube wall is provided with ventilating opening upwards, make the air-flow after purification from bottom to top in purification inoculation chamber flowing, prevent indoor gas convection current.
6. a kind of method reducing edible mushroom factory inoculation pollution rate according to claim 1, is characterized in that, enter the gas pressure intensity of purification inoculation chamber at least higher than the gas pressure intensity 30Pa of purification inoculation outdoor.
CN201510203294.2A 2015-04-24 2015-04-24 Method for reducing inoculating pollution rate of edible fungi factory Pending CN104823711A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115250827A (en) * 2022-08-24 2022-11-01 江苏菇本堂生物科技股份有限公司 Method for comprehensively preventing and treating velvet antler mushroom industrial production pollution

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101816257A (en) * 2010-03-26 2010-09-01 镇江市丹徒区正东生态农业发展中心 100-stage cleaning laminar flow inoculation under barotropic atmosphere during edible fungus industrial cultivation
CN202238075U (en) * 2011-10-18 2012-05-30 徐仰德 Low-temperature biological operation device
US8376821B2 (en) * 2008-01-10 2013-02-19 Mettler-Toledo Ag Work cabinet with a printer arranged outside the cabinet
EP2583752A1 (en) * 2011-10-21 2013-04-24 Yang-Te Hsu Laminar flow device
CN104429587A (en) * 2013-09-19 2015-03-25 王新军 Highly-pure aseptic inoculation device and inoculation room equipped with same

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8376821B2 (en) * 2008-01-10 2013-02-19 Mettler-Toledo Ag Work cabinet with a printer arranged outside the cabinet
CN101816257A (en) * 2010-03-26 2010-09-01 镇江市丹徒区正东生态农业发展中心 100-stage cleaning laminar flow inoculation under barotropic atmosphere during edible fungus industrial cultivation
CN202238075U (en) * 2011-10-18 2012-05-30 徐仰德 Low-temperature biological operation device
EP2583752A1 (en) * 2011-10-21 2013-04-24 Yang-Te Hsu Laminar flow device
CN104429587A (en) * 2013-09-19 2015-03-25 王新军 Highly-pure aseptic inoculation device and inoculation room equipped with same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115250827A (en) * 2022-08-24 2022-11-01 江苏菇本堂生物科技股份有限公司 Method for comprehensively preventing and treating velvet antler mushroom industrial production pollution

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Application publication date: 20150812

RJ01 Rejection of invention patent application after publication