CN104807917A - Method for measuring contents of fructose and glucose in honey - Google Patents
Method for measuring contents of fructose and glucose in honey Download PDFInfo
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- CN104807917A CN104807917A CN201510242702.5A CN201510242702A CN104807917A CN 104807917 A CN104807917 A CN 104807917A CN 201510242702 A CN201510242702 A CN 201510242702A CN 104807917 A CN104807917 A CN 104807917A
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Abstract
The invention discloses a method for measuring the contents of fructose and glucose in honey. The method comprises the following steps: 1, pretreating a sample, namely taking 5g of sample, dissolving the sample with 30ml of water, carrying out constant-volume processing on the dissolved sample to 100ml with acetonitrile, and filtering for later use; 2, carrying out analysis by using high performance liquid chromatography, wherein parameters for analysis by using the high performance liquid chromatography are set as follows: a flowing phase is acetonitrile-water, a flow velocity is 1ml/min, the column temperature is 40 DEG C, and a chromatographic column is Thermo Acclaim mixed medium chromatographic column, 4.6 mu. m*250mm, 5 mu. m; the volume ratio of acetonitrile to water in the flowing phase is 60: 40 to 80: 20. The invention aims at providing the method for measuring the fructose and the glucose in the honey, which is high in separating efficiency and accurate in measurement.
Description
Technical field
The present invention relates to detection field, particularly the assay method of a kind of Fructose in Honey and glucose content.
Background technology
Liquid chromatography for measuring Fructose in Honey, glucose, sucrose, maltose content is adopted in GB/T18932.22-2003 " assay method of Fructose in Honey, glucose, sucrose, maltose content ", the method measures accurately, main peak goes out clear, each component in peak and can clearly be separated, as GB, although have the Stability and veracity of height, what be still improved on measuring accuracy may.
Summary of the invention
The object of the present invention is to provide the assay method that a kind of separation efficiency is high, measure Fructose in Honey and glucose content accurately.
Technical scheme of the present invention is: the assay method of a kind of Fructose in Honey and glucose content, comprises the following steps:
Step 1: sample pretreatment, is settled to 100ml with acetonitrile after the water-soluble solution of sample thief 5g 30ml, for subsequent use after filtering;
Step 2: adopt high performance liquid chromatography to analyze;
The optimum configurations that high performance liquid chromatography carries out analyzing is as follows:
Mobile phase: acetonitrile-water; Flow velocity: 1ml/min column temperature: 40 DEG C
Chromatographic column: Thermo Acclaim mixed-matrix chromatographic column, 4.6 μm × 250mm, 5 μm; In described mobile phase, the volume ratio of acetonitrile and water is 60:40 ~ 80:20.
In the assay method of above-mentioned Fructose in Honey and glucose content, the model of the high performance liquid chromatograph that described step 2 is used is: hermo U3000 high performance liquid chromatograph.
In the assay method of above-mentioned Fructose in Honey and glucose content, described mobile phase adopts gradient elution, and the parameter of gradient elution is:
When 0 minute, in mobile phase, the volume ratio of acetonitrile and water is 60:40;
When 8 minutes, in mobile phase, the volume ratio of acetonitrile and water is 65:35;
When 11 minutes, in mobile phase, the volume ratio of acetonitrile and water is 70:30;
When 11 minutes to 11.1 minutes, the volume ratio of acetonitrile and water changes to 80:20;
When 11.1 minutes to 30 minutes, the volume ratio of acetonitrile and water is invariable is 80:20;
0 minute to 8 minutes and 8 minutes to 11 minutes periods acetonitrile and the volume ratio of water increase with constant speed.
Beneficial effect of the present invention is as follows:
Adopt such scheme effectively can improve the analysis precision of fructose and glucose.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is described in further detail, but does not form any limitation of the invention.
Embodiment 1
An assay method for Fructose in Honey and glucose content, comprises the following steps:
Step 1: sample pretreatment, is settled to 100ml with acetonitrile after the water-soluble solution of sample thief 5g 30ml, for subsequent use after filtering;
Step 2: adopt high performance liquid chromatography to analyze;
The optimum configurations that high performance liquid chromatography carries out analyzing is as follows:
Hermo U3000 high performance liquid chromatograph
Mobile phase: acetonitrile-water (gradient elution); Flow velocity: 1ml/min column temperature: 40 DEG C
Chromatographic column: Thermo Acclaim mixed-matrix chromatographic column, 4.6 μm × 250mm, 5 μm; In described mobile phase, the volume ratio of acetonitrile and water is 60:40 ~ 80:20.
The parameter of gradient elution is:
When 0 minute, in mobile phase, the volume ratio of acetonitrile and water is 60:40;
When 8 minutes, in mobile phase, the volume ratio of acetonitrile and water is 65:35;
When 11 minutes, in mobile phase, the volume ratio of acetonitrile and water is 70:30;
When 11 minutes to 11.1 minutes, the volume ratio of acetonitrile and water changes to 80:20;
When 11.1 minutes to 30 minutes, the volume ratio of acetonitrile and water is invariable is 80:20;
0 minute to 8 minutes and 8 minutes to 11 minutes periods acetonitrile and the volume ratio of water increase with constant speed.
The preparation of testing sample
7.1 extraction steps in GB/T18932.22-2003 are adopted to prepare testing sample.
Comparative example 1
7.2 determination steps in GB/T18932.22-2003 are adopted to measure.
Test result:
The fructose appearance time of embodiment 1 is 6.225min, and glucose appearance time is 9.357min;
The pyroglutamate time of comparative example 1 is 7.851min, and glucose appearance time is 9.254min;
By the method for embodiment 1, go out peak and be separated more obvious, the accuracy of test can be improved.
Above-describedly be only preferred embodiment of the present invention, all do within the scope of the spirit and principles in the present invention any amendment, equivalently to replace and improvement etc., all should be included within protection scope of the present invention.
Claims (3)
1. an assay method for Fructose in Honey and glucose content, is characterized in that, comprises the following steps:
Step 1: sample pretreatment, is settled to 100ml with acetonitrile after the water-soluble solution of sample thief 5g 30ml, for subsequent use after filtering;
Step 2: adopt high performance liquid chromatography to analyze;
The optimum configurations that high performance liquid chromatography carries out analyzing is as follows:
Mobile phase: acetonitrile-water; Flow velocity: 1ml/min column temperature: 40 DEG C
Chromatographic column: Thermo Acclaim mixed-matrix chromatographic column, 4.6 μm × 250mm, 5 μm; In described mobile phase, the volume ratio of acetonitrile and water is 60:40 ~ 80:20.
2. the assay method of Fructose in Honey according to claim 1 and glucose content, is characterized in that, the model of the high performance liquid chromatograph that described step 2 is used is: hermo U3000 high performance liquid chromatograph.
3. the assay method of Fructose in Honey according to claim 2 and glucose content, is characterized in that, described mobile phase adopts gradient elution, and the parameter of gradient elution is:
When 0 minute, in mobile phase, the volume ratio of acetonitrile and water is 60:40;
When 8 minutes, in mobile phase, the volume ratio of acetonitrile and water is 65:35;
When 11 minutes, in mobile phase, the volume ratio of acetonitrile and water is 70:30;
When 11 minutes to 11.1 minutes, the volume ratio of acetonitrile and water changes to 80:20;
When 11.1 minutes to 30 minutes, the volume ratio of acetonitrile and water is invariable is 80:20;
0 minute to 8 minutes and 8 minutes to 11 minutes periods acetonitrile and the volume ratio of water increase with constant speed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510242702.5A CN104807917A (en) | 2015-05-12 | 2015-05-12 | Method for measuring contents of fructose and glucose in honey |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510242702.5A CN104807917A (en) | 2015-05-12 | 2015-05-12 | Method for measuring contents of fructose and glucose in honey |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104807917A true CN104807917A (en) | 2015-07-29 |
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| CN201510242702.5A Pending CN104807917A (en) | 2015-05-12 | 2015-05-12 | Method for measuring contents of fructose and glucose in honey |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114088833A (en) * | 2021-11-17 | 2022-02-25 | 广州浩宇生物科技有限公司 | Method for rapidly detecting glucose content in food |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005080515A (en) * | 2003-09-04 | 2005-03-31 | Suntory Ltd | NEW alpha-GLUCOSIDASE AND METHOD FOR PRODUCING THE SAME |
-
2015
- 2015-05-12 CN CN201510242702.5A patent/CN104807917A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005080515A (en) * | 2003-09-04 | 2005-03-31 | Suntory Ltd | NEW alpha-GLUCOSIDASE AND METHOD FOR PRODUCING THE SAME |
Non-Patent Citations (5)
| Title |
|---|
| THERMO FISHER SCIENTIFIC INC: "Sugars in a Sports Beverage Using Acclaim Trinity P2 in HILIC Mode", 《THERMO SCIENTIFIC》 * |
| THERMO FISHER SCIENTIFIC INC: "Sugars in Honey Using Accucore 150-Amide-HILIC", 《THERMO SCIENTIFIC》 * |
| 中华人民共和国国家质量监督检验检疫总局 中国国家标准化管理委员会: "《中华人民共和国国家标准 GB/T 22221-2008》", 16 May 2008 * |
| 国家质量监督检验检疫总局: "《GB/T 18932.22-2003》", 1 January 2003 * |
| 张书芬 等: "液相色谱示差折光法测定蜂蜜中的果糖、葡萄糖、蔗糖和麦芽糖", 《食品科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114088833A (en) * | 2021-11-17 | 2022-02-25 | 广州浩宇生物科技有限公司 | Method for rapidly detecting glucose content in food |
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Application publication date: 20150729 |