CN104799885A - Tissue cage model for collecting subcutaneous tissue fluid of beagle and using method - Google Patents
Tissue cage model for collecting subcutaneous tissue fluid of beagle and using method Download PDFInfo
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- CN104799885A CN104799885A CN201510237132.0A CN201510237132A CN104799885A CN 104799885 A CN104799885 A CN 104799885A CN 201510237132 A CN201510237132 A CN 201510237132A CN 104799885 A CN104799885 A CN 104799885A
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Abstract
The invention relates to a tissue cage model for collecting the subcutaneous tissue fluid of beagle and a using method. A medical silicone tube is adopted as a main body of the tissue cage model, wherein at least four uniformly distributed small tissue fluid exchange holes with diameters of 2mm are formed in each of the two ends of the medical silicone tube; two end openings of the medical silicone tube are sealed by medical silicone round sheets; the tissue cage model has an external diameter of 16.0 to 20.0mm, an internal diameter of 11.0 to 15.0mm, a length of 63 to 67mm and a volume of about 6.0 to 11.0cm<3>. After being shaped, cleaned and sterilized, the model is implanted into the subcutaneous tissue layer on the two sides of the back of the beagle in a surgical operation manner, and after 4 to 5 weeks, clear yellowish fluid in a tissue cage of an uninfected wound is tissue fluid. A disposable injector externally pierces the tissue cage to extract the tissue fluid for researches in related directions of in-vivo tissue fluid pharmacokinetics and pharmacodynamics of drugs, in-vivo antibacterial activity of antibiotics, drug resistance and the like.
Description
Technical field
The present invention relates to drug test research field, particularly relate to a kind of tissue cage model collecting animal skins undertissue liquid.
Background technology
Entered the medicine in body by different modes of administration, first arriving blood by absorbing, being then transported to each target organ of body, target tissue again.What tradition pharmacokinetic studies detected is the drug level of blood plasma or serum.And there is different infiltration ratios in blood plasma and organ-tissue, blood plasma or drug in blood serum concentration can not represent in reality completely organizes drug concentration.And another large body fluid of body, tissue fluid be but pathogenic microorganism or its toxin infringement parenchyma must through place, equally also be the ground at various kinds of drug treatment damaged tissues or cell and the place playing drug effect.Thus, we are when carrying out pharmaceutical research to medicine, and it is of equal importance that blood and tissue fluid Chinese medicine move pharmacodynamics, and tissue fluid drug concentration more can actual response target site drug level and therapeutic effect.Tissue fluid is not identical with blood constituent, medicine enters tissue fluid by various factors such as the chemical constitution of medicine own, ionization property, permeability, protein binding rates by blood, thus there is larger gap in tissue or tissue fluid drug concentration and blood drug concentration, and characteristics of pharmacokinetics is also and incomplete same in these two kinds of body fluid for medicine.So it is not only move one of pharmacodynamic study as blood Chinese medicine to supplement effectively that drug entities liquid Chinese medicine moves pharmacodynamic study, but when thoroughly evaluating pharmacopathology feature an indispensable part.
What is more important, for antibiotic medicine research, uses tissue cage model, by injecting antibacterial in tissue cage, can carry out medicine in complete body dynamic-pharmacodynamics and the research of medicine Catastrophic selection window.Now, pathogen, antibacterials be same fixed-site interaction in animal body, closer to real treatment situation.In recent years, different genera animal (cattle, sheep, goat, rabbit, cat, etc.) tissue fluid pharmacokinetics and efficacy has relevant report.This mainly gives the credit to and the successful foundation of tissue cage model at different animals, this type of test is carried out between different target animals, systematic study medicine at different genera animal pharmacology characteristic and drug resistance, for medication reasonable in design provides accurate guidance.
Due to different animals physiological property, particularly epithelial properties is different, and the tissue cage model thus implanted in animal skins undertissue is not identical yet.At present, the tissue cage be applicable in the animal body such as cattle, rabbit has commodity selling, i.e. golf model.But this model volume is comparatively large, and be not suitable for skin comparatively closely with it beasle dog.Therefore, need to invent a kind of novel tissue cage model, for beasle dog in-vivo tissue liquid correlational study.
Summary of the invention
The object of the present invention is to provide a kind of the tissue cage model and the using method thereof that are applicable to beasle dog in-vivo tissue liquid correlational study, this model is by after manual manufacture molding, implant beasle dog subcutaneous tissue, limpid yellow tissue fluid can be collected after one period of convalescent period.
The object of the invention is to be achieved through the following technical solutions:
A kind of tissue cage model collecting beasle dog subcutaneous tissue liquid, select medical silicone tube as the main body of this tissue cage model, respectively offer at least 4 equally distributed tissue fluids at the two ends of medical silicone tube and exchange aperture, the diameter that this tissue fluid exchanges aperture is 2mm, the two-port of medical silicone tube is by medical silica-gel disk seal closure, the outside dimension of this tissue cage model is 16.0 ~ 20.0 mm, internal diameter size is 11.0 ~ 15.0 mm, tissue cage model length is 63 ~ 67mm, and volume is about 6.0 ~ 11.0cm
3.
The outside dimension of this tissue cage model is 18.0 mm, and internal diameter size is 13.0 mm, and tissue cage model length is 65 mm, and volume is 8.0 cm
3.
The two ends of this tissue cage model respectively offer equally distributed 8 tissue fluids and exchange aperture, and it is 50 ~ 50.5 mm that tissue fluid exchanges total surface area
2.
Described medical silica-gel disk first uses nylon line suture in the two-port of medical silicone tube, then uses 705 silica gel bondings to close gap.
A kind of using method of collecting the tissue cage model of beasle dog subcutaneous tissue liquid, after the tissue cage modelling of collection beasle dog subcutaneous tissue liquid described above completes sizing, first clean up, then bind up with gauze, be placed in soaking disinfection in bromogeramine solution, post-drying of spending the night; By operation method, tissue cage is implanted both sides, dog back subcutaneous tissue, its key step is: dog body is bound
injection pentobarbital anaesthetizes sb. generally
hair, iodine disinfection and de-iodine are shaved by art portion
injection procaine carries out local anesthesia
scalpel cuts skin surface, carries out implanting tissue cage after blunt separation with tweezers and shears
wound suture, coats tetracycline sterilization
intramuscular injection penicillin injection anti-inflammation
restoration ecosystem, after 4 ~ 5 weeks, is extracted clear yellow liquid with syringe out, is tissue fluid in tissue cage, shows that tissue cage model is successfully set up, can test.
The present invention compared with prior art has following outstanding advantages:
1, tissue cage model described in the invention, selected material is medical silicone tube, has good biocompatibility, and low price, convenient acquisition;
2, tissue cage model described in the invention, manufacturing process is simple, and make by hand for pure, applicable laboratory is promoted the use of;
3, the present invention is by control experiment, determines best making dog tissue cage used silica gel pipe internal-and external diameter specification, better instructs the making of dog tissue cage model;
4, tissue cage model described in the invention, after surgically implanting beasle dog subcutaneous tissue, clear yellow tissue fluid can be collected after 4-5 week, be applicable to beasle dog in-vivo tissue liquid correlational study completely, and do not have any tissue cage model to reach this requirement in the market.
Accompanying drawing explanation
Fig. 1 is tissue cage schematic diagram in kind;
Fig. 2 is silica gel disk schematic diagram.
Wherein: 1, medical silicone tube, 2, the two ends of medical silicone tube, 3, exchange aperture, 4, medical silica-gel disk.
Detailed description of the invention
The invention is further illustrated by the following examples.
Embodiment 1
The main body of this tissue cage model selects medical silicone tube (1), and biocompatibility is better.Tissue cage model specification is: external diameter 18.0 mm, internal diameter 13.0 mm, and being about is 65 ± 2 mm, and volume is about 8.0 cm
3; Each at the two ends (2) of medical silicone tube to make a call to 8 diameters be 2.0 mm apertures, makes tissue cage and environmental liquids exchange area be 50.3 mm
2.Medical silica-gel disk (4) first uses nylon line suture in the two ends (2) of medical silicone tube, then uses 705 silica gel bondings to close gap.
After the tissue cage completed cleans up, to spend the night soaking disinfection with bromogeramine solution, for subsequent use after drying.After first beasle dog anaesthetizes sb. generally with pentobarbital sodium, hair is shaved at back, both sides, iodine tincture disinfection and ethanol take off iodine, with procaine hydrochloride, local anesthesia is carried out to art, with scalpel otomy portion skin under aseptic condition, with tweezers and operating scissors, blunt separation is carried out to wound again, the wound size degree of depth is suitable fills in subcutaneous tissue afterwards tissue cage, finally use suture wound, and on sew up wound, coat tetracycline ointment in case wound infection, intragluteal injection penicillin sodium injection, carries out whole body anti-inflammation.Through intensive care in 1 week, wound healing, removed stitching thread after 10 days.Tissue cage outer wrapping one deck connective tissue after 4-5 week, tissue fluid enters tissue cage collect by being positioned at tissue cage two ends aperture.Outside skin, thrust sucking-off clear yellow tissue fluid in tissue cage with Dispoable medical syringe, illustrate that tissue cage model is successfully set up, can correlation test be carried out.
Embodiment 2
Best silica gel tube specification is determined by control experiment:
Select the medical silicone tube of different size, make tissue cage model according to embodiment 1, and be implanted into dog subcutaneous tissue of back, continuous observation 1-2 month, and collection organization's liquid, assessment tissue cage model success rate, thus select the optimum medical silicone tube size of making organize models.Result is as following table:
Claims (5)
1. collect the tissue cage model of beasle dog subcutaneous tissue liquid for one kind, it is characterized in that the main body selecting medical silicone tube as this tissue cage model, respectively offer at least 4 equally distributed tissue fluids at the two ends of medical silicone tube and exchange aperture, the diameter that this tissue fluid exchanges aperture is 2mm, the two-port of medical silicone tube is by medical silica-gel disk seal closure, the outside dimension of this tissue cage model is 16.0 ~ 20.0 mm, internal diameter size is 11.0 ~ 15.0 mm, tissue cage model length is 63 ~ 67mm, and volume is about 6.0 ~ 11.0cm
3.
2. the tissue cage model of collection beasle dog subcutaneous tissue liquid according to claim 1, it is characterized in that the outside dimension of this tissue cage model is 18.0 mm, internal diameter size is 13.0 mm, and tissue cage model length is 65 mm, and volume is 8.0 cm
3.
3. the tissue cage model of collection beasle dog subcutaneous tissue liquid according to claim 1, it is characterized in that the two ends of this tissue cage model respectively offer equally distributed 8 tissue fluids and exchange aperture, it is 50 ~ 50.5 mm that tissue fluid exchanges total surface area
2.
4. the tissue cage model of collection beasle dog subcutaneous tissue liquid according to claim 1, is characterized in that described medical silica-gel disk first uses nylon line suture in the two-port of medical silicone tube, then uses 705 silica gel bondings to close gap.
5. collect the using method of the tissue cage model of beasle dog subcutaneous tissue liquid for one kind, it is characterized in that: after the tissue cage modelling as any one collection beasle dog subcutaneous tissue liquid of above-mentioned Claims 1-4 completes sizing, first clean up, bind up with gauze again, be placed in soaking disinfection in bromogeramine solution, post-drying of spending the night; By operation method, tissue cage is implanted both sides, dog back subcutaneous tissue, its key step is: dog body is bound
injection pentobarbital anaesthetizes sb. generally
hair, iodine disinfection and de-iodine are shaved by art portion
injection procaine carries out local anesthesia
scalpel cuts skin surface, carries out implanting tissue cage after blunt separation with tweezers and shears
wound suture, coats tetracycline sterilization
intramuscular injection penicillin injection anti-inflammation
restoration ecosystem, after 4 ~ 5 weeks, is extracted clear yellow liquid with syringe out, is tissue fluid in tissue cage, shows that tissue cage model is successfully set up, can test.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510237132.0A CN104799885A (en) | 2015-05-12 | 2015-05-12 | Tissue cage model for collecting subcutaneous tissue fluid of beagle and using method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510237132.0A CN104799885A (en) | 2015-05-12 | 2015-05-12 | Tissue cage model for collecting subcutaneous tissue fluid of beagle and using method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110495908A (en) * | 2019-09-18 | 2019-11-26 | 浙江中医药大学 | A kind of in-vivo tissue Ionization chamber and tissue liquor collecting device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030130744A1 (en) * | 1991-08-12 | 2003-07-10 | Bonutti Peter M. | Tissue cage |
| CN204600534U (en) * | 2015-05-12 | 2015-09-02 | 苏州圣苏新药开发有限公司 | Collect the tissue cage model of beasle dog subcutaneous tissue liquid |
-
2015
- 2015-05-12 CN CN201510237132.0A patent/CN104799885A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030130744A1 (en) * | 1991-08-12 | 2003-07-10 | Bonutti Peter M. | Tissue cage |
| CN204600534U (en) * | 2015-05-12 | 2015-09-02 | 苏州圣苏新药开发有限公司 | Collect the tissue cage model of beasle dog subcutaneous tissue liquid |
Non-Patent Citations (2)
| Title |
|---|
| 杨雨辉等: "在猪组织笼内恩诺沙星对大肠杆菌的药效研究", 《东北农业大学学报》 * |
| 王国俊等: "双黄连联用左氧氟沙星对兔组织笼感染模型中金黄色葡萄球菌ATCC29213 耐药性的影响", 《医药导报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110495908A (en) * | 2019-09-18 | 2019-11-26 | 浙江中医药大学 | A kind of in-vivo tissue Ionization chamber and tissue liquor collecting device |
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Application publication date: 20150729 |