CN104783224A - Method for preparing minced fillet - Google Patents
Method for preparing minced fillet Download PDFInfo
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- CN104783224A CN104783224A CN201510203307.6A CN201510203307A CN104783224A CN 104783224 A CN104783224 A CN 104783224A CN 201510203307 A CN201510203307 A CN 201510203307A CN 104783224 A CN104783224 A CN 104783224A
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Abstract
The invention discloses a method for preparing minced fillet. The method comprises the following steps: preparing fish, taking the fish meat, bleaching and blending, wherein sea cucumber essence, agar ester and starch are added after the bleaching step, and subsequently the blending step is performed; after the blending step, protein complex phosphoesterasum is added and uniformly stirred for incubation, so that a composite raw material can be prepared from minced fillet protein in minced fillet, agar ester, sea cucumber essence and starch, intramolecular and intermolecular cross-linking and coating of minced fillet protein, sea cucumber protein of sea cucumber essence, sea cucumber polysaccharide of sea cucumber essence and sea cucumber saponin of sea cucumber essence with sea cucumber saponin coating can be achieved, and thus minced fillet can be prepared. The method has the technical effects that not only is the taste improved, but also the influence to human body health caused by high salt is avoided; due to catalysis of the protein complex phosphoesterasum, the complex phosphoesterasum can be cross-linked and coated with protein in fish meat, and the elastic flavor and the nutrition of the minced fillet are further increased; the agar ester has functions of improving the immunity and preventing viruses, and sulfated polysaccharide can be relatively easily cross-linked with protein.
Description
Technical field
The present invention relates to food making method, be specifically related to the rotten preparation method of a kind of fish.
Background technology
Surimi product extensively gets consumer reception, and researches and develops novel, healthy, nutritious surimi product significant.Most Yu Mi processing enterprise still adopts high salt to improve the gelling performance of surimi product, unfavorable health.It is crosslinked to prepare the good surimi product of less salt, gelation that Some Enterprises starts to apply the rotten albumen of transglutaminases catalyze fish.
Summary of the invention
The object of the present invention is to provide the rotten preparation method of a kind of fish, the method has prepared a kind of less salt and nutritious fish is rotten.
One of this technical scheme is as follows:
The rotten preparation method of a kind of fish, comprises standby fish step, gets flesh of fish step, rinse step and arena and to burst step; After rinse step, add sea cucumber elite, agar-agar ester and starch carry out again beating routed step, after step is burst in arena, add albumen complex enzyme and stir evenly incubation again, to make the rotten albumen of the fish in fish gruel, agar-agar ester, sea cucumber elite, starch composition compound material, in the molecule realizing the rotten albumen of fish, the sea cucumber albumen of sea cucumber elite, the sea cucumber polysaccharide of sea cucumber elite, the selenka of sea cucumber elite and intermolecular cross-linking and bag by selenka, thus prepare fish gruel.
Among one embodiment: every hectogram flesh of fish adds sea cucumber elite 2-5 gram, agar-agar ester 2-5 gram, starch 10-30 gram, albumen complex enzyme 0.1-2 gram.
Among one embodiment: described albumen complex enzyme comprises glutamine transaminage and protein-crosslinking enzyme, the part by weight of described glutamine transaminage and protein-crosslinking enzyme is 1:0.5-1.
Among one embodiment: described protein-crosslinking enzyme extracts from Chinese yam, described protein-crosslinking enzyme comprises polyphenol oxidase and peroxidase.
Among one embodiment: the extracting method of described protein-crosslinking enzyme comprises:
Chinese yam is removed the peel;
Add phosphate buffer, after homogenate, waste gets supernatant;
Cold acetone is added, centrifuging and taking sediment after stirring toward supernatant;
Cold wind dries up sediment;
Sediment adds phosphate buffer and obtains protein-crosslinking enzyme.
Among one embodiment: described agar-agar ester is the accessory substance collected by gelose preparation process; The preparation technology of described agar-agar ester is as follows:
After agar-agar adds water, heating makes it dissolve, and then adds DEAE-celluosic resin, then at the uniform velocity stirs, and then leaves standstill insulation, then suction filtration, then washing leaching cake while hot;
Be soaked in by filter cake in NaCl solution, then at the uniform velocity stir, then suction filtration while hot, then filtrate is reclaimed;
Enough hemodialysis after filtrate is concentrated, concentrated, dry agar-agar ester.
Among one embodiment: adding albumen complex enzyme and within 10-100 minute, carrying out incubation in the placement of 20-50 degree scope inside holding after stirring evenly.
Among one embodiment: first place 10-20 minute at 35-45 degree scope inside holding after interpolation albumen complex enzyme, play catalytic crosslinking effect to allow glutamine transaminage and polyphenol oxidase; Then under 55-65 degree, be incubated 5-20 minute and allow the effect of peroxidase generation catalytic crosslinking, allow the sea cucumber albumen of the rotten albumen of fish in the gruel of mixing fish, sea cucumber elite, the sea cucumber polysaccharide of sea cucumber elite, the selenka of sea cucumber elite molecule in and intermolecular cross-linking and bag by selenka.
This technical scheme two as follows:
The rotten preparation method of a kind of fish, comprises standby fish step, gets flesh of fish step, rinse step and arena and burst step to prepare fish gruel; In fish gruel, add sea cucumber elite, agar-agar ester, starch and albumen complex enzyme and stir evenly incubation again, to make the rotten albumen of the fish in fish gruel, agar-agar ester, sea cucumber elite, starch composition compound material, in the molecule realizing the rotten albumen of fish, the sea cucumber albumen of sea cucumber elite, the sea cucumber polysaccharide of sea cucumber elite, the selenka of sea cucumber elite and intermolecular cross-linking and bag by selenka, thus it is rotten to prepare mixing fish.
Among one embodiment: beat add sea cucumber elite, agar-agar ester, starch in fish gruel after and burst; Arena adds albumen complex enzyme after bursting and stirs evenly incubation again.
Among one embodiment: every hectogram flesh of fish adds sea cucumber elite 2-5 gram, agar-agar ester 2-5 gram, starch 10-30 gram, albumen complex enzyme 0.1-2 gram.
Among one embodiment: described albumen complex enzyme comprises glutamine transaminage and protein-crosslinking enzyme, the part by weight of described glutamine transaminage and protein-crosslinking enzyme is 1:0.5-1.
Among one embodiment: described protein-crosslinking enzyme extracts from Chinese yam, described protein-crosslinking enzyme comprises polyphenol oxidase and peroxidase.
Among one embodiment: the extracting method of described protein-crosslinking enzyme comprises:
Chinese yam is removed the peel;
Add phosphate buffer, after homogenate, waste gets supernatant;
Cold acetone is added, centrifuging and taking sediment after stirring toward supernatant;
Cold wind dries up sediment;
Sediment adds phosphate buffer and obtains protein-crosslinking enzyme.
Among one embodiment: described agar-agar ester is the accessory substance collected by gelose preparation process; The preparation technology of described agar-agar ester is as follows:
After agar-agar adds water, heating makes it dissolve, and then adds DEAE-celluosic resin, then at the uniform velocity stirs, and then leaves standstill insulation, then suction filtration, then washing leaching cake while hot;
Be soaked in by filter cake in NaCl solution, then at the uniform velocity stir, then suction filtration while hot, then filtrate is reclaimed;
Enough hemodialysis after filtrate is concentrated, concentrated, dry agar-agar ester.
Among one embodiment: adding albumen complex enzyme and within 10-100 minute, carrying out incubation in the placement of 20-50 degree scope inside holding after stirring evenly.
Among one embodiment: first place 10-20 minute at 35-45 degree scope inside holding after interpolation albumen complex enzyme, play catalytic crosslinking effect to allow glutamine transaminage and polyphenol oxidase; Then under 55-65 degree, be incubated 5-20 minute and allow the effect of peroxidase generation catalytic crosslinking, allow the sea cucumber albumen of the rotten albumen of fish in the gruel of mixing fish, sea cucumber elite, the sea cucumber polysaccharide of sea cucumber elite, the selenka of sea cucumber elite molecule in and intermolecular cross-linking and bag by selenka.
It can produce following technique effect:
1, on production surimi product, the use of albumen complex enzyme instead of and utilizes high salt to promote the gelling performance of surimi product traditionally, not only increases mouthfeel and more avoids the healthy impact of high salt pair; By agar-agar ester, sea cucumber elite, starch add in fish gruel, by the catalysis of albumen complex enzyme make its with the flesh of fish in albumen carry out crosslinked bag quilt, further increase elasticity local flavor and the nutrition of fish gruel; Agar-agar ester is the sulfated polysaccharide in marine red alga, has and improves immunity and antiviral functions, and sulfated polysaccharide is easier and protein-crosslinking.
2, the extraction process of agar-agar ester, sea cucumber elite, protein-crosslinking enzyme saves its cost simply more.
3, every hectogram flesh of fish adds sea cucumber elite 2-5 gram, agar-agar ester 2-5 gram, starch 10-30 gram, and cross-linking effect is better, and mouthfeel is good.
4, albumen complex enzyme comprises glutamine transaminage and protein-crosslinking enzyme, protein-crosslinking enzyme comprises polyphenol oxidase and peroxidase, and multiple enzyme is used in combination, and cross-linking effect is better.
Detailed description of the invention
Embodiment one
The rotten preparation method of a kind of fish, comprising:
Standby fresh fish step;
Peeling, extracts fresh flesh of fish step;
Adopting rinsing machine to carry out rinse step, for removing water soluble protein in the flesh of fish, pigment, smell and fat, improving elasticity and the whiteness of the flesh of fish; Clear water rinsing or rare saline-alkali water rinsing are such as selected in this rinsing;
Add sea cucumber elite, agar-agar ester and starch; Wherein: every hectogram flesh of fish adds sea cucumber elite 2-5 gram, agar-agar ester 2-5 gram, starch 10-30 gram; Described sea cucumber elite comprises sea cucumber protein 15-25 weight portion, sea cucumber polysaccharide 15-25 weight portion and selenka 40-60 weight portion, as sea cucumber protein 20 weight portion, sea cucumber polysaccharide 20 weight portion and selenka 50 weight portion, or, sea cucumber albumen about 17%, sea cucumber polysaccharide about 20%, selenka 52%;
Adopt mixing and kneading machine to carry out arena to burst, to prepare fish gruel, beating bursts is commonly called as " mixing arena ", by stirring and abrasive action, flesh of fish muscle fiber is destroyed further, for good condition is created in the abundant stripping of salting-in protein;
Add albumen complex enzyme and stir evenly, incubation, to make the rotten albumen of the fish in fish gruel, agar-agar ester, sea cucumber elite, starch composition compound material, realize fish rotten albumen, the sea cucumber albumen of sea cucumber elite, the sea cucumber polysaccharide of sea cucumber elite, the selenka of sea cucumber elite, in their molecule and intermolecular cross-linking and bag by selenka, thus it is rotten to prepare Novel advanced fish; Described albumen complex enzyme comprises glutamine transaminage and protein-crosslinking enzyme; Described protein-crosslinking enzyme extracts from Chinese yam, and described protein-crosslinking enzyme comprises polyphenol oxidase and peroxidase.The gruel of every hectogram fish adds albumen complex enzyme 0.1-2 gram, glutamine transaminage and protein-crosslinking enzyme 1:0.5-1.
The above-mentioned method extracting protein-crosslinking enzyme from Chinese yam comprises:
Fresh Chinese yam is removed the peel;
Add phosphate buffer, after homogenate, waste gets supernatant;
Cold acetone is added, slowly centrifuging and taking sediment after stirring a period of time toward supernatant;
Cold wind dries up sediment;
Sediment adds phosphate buffer and obtains protein-crosslinking enzyme, after measured its polyphenol oxidase and peroxidase activity higher, Cord blood, as-20 to 4 degree of preservations.
Above-mentioned sea cucumber elite is extracted from sea cucumber cooking liquor, extracting method comprises: sea cucumber cooking liquor is crossed AB-8 type macroporous resin column, collect (60-80) %, as 70% ethanol elution component, obtain sea cucumber elite, after measured wherein containing sea cucumber albumen about 17%, sea cucumber polysaccharide about 20%, selenka 52%.Fresh sea cucumber is because of easy autolyze, and namely carry out boiling after slaughtering with its autolytic enzyme of inactivation, this process produces a large amount of sea cucumber cooking liquors, is taken as discharge of wastewater.In the present embodiment, extract from sea cucumber cooking liquor and obtain the sea cucumber elite being rich in selenka, sea cucumber albumen and sea cucumber polysaccharide, cost is low.
Above-mentioned agar-agar ester is the accessory substance collected by gelose preparation process; The preparation technology of described agar-agar ester is as follows:
After agar-agar adds water, heating makes it dissolve, and then adds pretreated DEAE-celluosic resin, then at the uniform velocity stirs, at the uniform velocity stir 0.5-3 hour (preferably at 70 DEG C, at the uniform velocity stirring 2h with rotating speed 15r/min) at 60 DEG C-80 DEG C;
Then, after leaving standstill insulation 0.3-0.7 hour (as 0.5 hour), suction filtration while hot, then washing leaching cake, select 80-95 degree distilled water to wash (as 90 DEG C of distilled water);
Be soaked in by filter cake in 1.0-3.0mol/L (best 2.0mol/L) NaCl solution, then at the uniform velocity stir (at 60 DEG C-80 DEG C, at the uniform velocity stirring 1-3 hour), then suction filtration while hot, then filtrate is reclaimed;
Enough hemodialysis after filtrate is concentrated, 40 DEG C-60 DEG C (as 50 DEG C) are concentrated, dry obtains agar-agar ester, and it has antiviral activity after measured.The agar extracted from marine red alga, comprises two large divisions, and one is the algal polysaccharides (agarose) that sulfate radical content is very low, and it two is the seaweed polysaccharide sulfate (agar-agar ester) that sulfate radical content is higher.The process extracting agarose from agar produces a large amount of agar-agar ester, and be not utilized at present, the present embodiment is used, and cost is low.
The preparation technology of another agar-agar ester is as follows: preparation technology's difference of it and a upper agar-agar ester is: stirring condition is: at 65 DEG C, at the uniform velocity stir 1 hour; Boiling water is selected to wash.
The preferred version of incubation: place at 20-50 degree scope inside holding after stirring evenly carry out an incubation in 10-100 minute at interpolation albumen complex enzyme, allow the macromolecule internal such as albumen (fish rotten albumen, sea cucumber albumen), polysaccharide (agar-agar ester, sea cucumber polysaccharide, starch) and saponin(e in mixing fish gruel, between there is crosslinked and bag by selenka.
The preferred version of another kind of incubation: first insulation placement allows glutamine transaminage and polyphenol oxidase play catalytic crosslinking effect for 10-20 minute under 40 degree after interpolation albumen complex enzyme; Then under 60 degree, 5-20 minute is incubated and allows the effect of peroxidase generation catalytic crosslinking, allow the macromolecule internal such as albumen (fish rotten albumen, sea cucumber albumen), polysaccharide (agar-agar ester, sea cucumber polysaccharide, starch) and saponin(e in mixing fish gruel, between there is crosslinked and bag by selenka, cross-linking effect is good, and mouthfeel is good.
Food protein is cross-linked and the crosslinked and dehydrogenate protein matter that Maillard reaction is cross-linked, disulfide bond crosslinking, glutamine transaminage are cross-linked, tyrosine residue causes can be divided into be cross-linked.The enzyme of catalysis food protein generation crosslinked action mainly contains TGase, polyphenol oxidase and peroxidase etc., and the catalytic mechanism between them is different, and formed complex enzyme with several different protein-crosslinking enzyme and carry out the rotten protein-crosslinking of fish, cross-linking effect is better.And except protein-protein can occur crosslinked except, protein and saponin(e also can carry out covalency and Non-covalent binding, and protein and polysaccharide Maillard reaction also can occur under certain condition and realize covalent cross-linking.
As required, also can comprise after the incubation of the rotten preparation method of a kind of fish: mix filling step, forming step and shortening step.
Embodiment two
It and embodiment one difference are: the rotten preparation method of a kind of fish, comprising:
Standby fresh fish step;
Peeling, extracts fresh flesh of fish step;
Rinsing machine is adopted to carry out rinse step;
Adopt mixing and kneading machine to carry out arena to burst;
Add sea cucumber elite, agar-agar ester, starch, beat and burst;
Add albumen complex enzyme, stir evenly, incubation, to make the rotten albumen of the fish in fish gruel, agar-agar ester, sea cucumber elite, starch composition compound material, realize fish rotten albumen, the sea cucumber albumen of sea cucumber elite, the sea cucumber polysaccharide of sea cucumber elite, the selenka of sea cucumber elite, in their molecule and intermolecular cross-linking and bag by selenka, thus it is rotten to prepare Novel advanced fish.
The above, be only present pre-ferred embodiments, therefore can not limit scope of the invention process according to this, the equivalence change namely done according to the scope of the claims of the present invention and description with modify, all should still belong in scope that the present invention contains.
Claims (10)
1. the rotten preparation method of fish, comprises standby fish step, gets flesh of fish step, rinse step and arena and to burst step; It is characterized in that: after rinse step, add sea cucumber elite, agar-agar ester and starch carry out again beating routed step, after step is burst in arena, add albumen complex enzyme and stir evenly incubation again, to make the rotten albumen of the fish in fish gruel, agar-agar ester, sea cucumber elite, starch composition compound material, in the molecule realizing the rotten albumen of fish, the sea cucumber albumen of sea cucumber elite, the sea cucumber polysaccharide of sea cucumber elite, the selenka of sea cucumber elite and intermolecular cross-linking and bag by selenka, thus prepare fish gruel.
2. the rotten preparation method of fish, comprises standby fish step, gets flesh of fish step, rinse step and arena and burst step to prepare fish gruel; It is characterized in that: in fish gruel, add sea cucumber elite, agar-agar ester, starch and albumen complex enzyme and stir evenly incubation again, to make the rotten albumen of the fish in fish gruel, agar-agar ester, sea cucumber elite, starch composition compound material, in the molecule realizing the rotten albumen of fish, the sea cucumber albumen of sea cucumber elite, the sea cucumber polysaccharide of sea cucumber elite, the selenka of sea cucumber elite and intermolecular cross-linking and bag by selenka, thus it is rotten to prepare mixing fish.
3. the rotten preparation method of a kind of fish according to claim 2, is characterized in that: beat add sea cucumber elite, agar-agar ester, starch in fish gruel after and burst; Arena adds albumen complex enzyme after bursting and stirs evenly incubation again.
4. the rotten preparation method of a kind of fish according to claim 1 and 2, is characterized in that: every hectogram flesh of fish adds sea cucumber elite 2-5 gram, agar-agar ester 2-5 gram, starch 10-30 gram, albumen complex enzyme 0.1-2 gram.
5. the rotten preparation method of a kind of fish according to claim 4, it is characterized in that: described albumen complex enzyme comprises glutamine transaminage and protein-crosslinking enzyme, the part by weight of described glutamine transaminage and protein-crosslinking enzyme is 1:0.5-1.
6. the rotten preparation method of a kind of fish according to claim 5, it is characterized in that: described protein-crosslinking enzyme extracts from Chinese yam, described protein-crosslinking enzyme comprises polyphenol oxidase and peroxidase.
7. the rotten preparation method of a kind of fish according to claim 6, is characterized in that: the extracting method of described protein-crosslinking enzyme comprises:
Chinese yam is removed the peel;
Add phosphate buffer, after homogenate, waste gets supernatant;
Cold acetone is added, centrifuging and taking sediment after stirring toward supernatant;
Cold wind dries up sediment;
Sediment adds phosphate buffer and obtains protein-crosslinking enzyme.
8. the rotten preparation method of a kind of fish according to claim 1 and 2, is characterized in that: described agar-agar ester is the accessory substance collected by gelose preparation process; The preparation technology of described agar-agar ester is as follows:
After agar-agar adds water, heating makes it dissolve, and then adds DEAE-celluosic resin, then at the uniform velocity stirs, and then leaves standstill insulation, then suction filtration, then washing leaching cake while hot;
Be soaked in by filter cake in NaCl solution, then at the uniform velocity stir, then suction filtration while hot, then filtrate is reclaimed;
Enough hemodialysis after filtrate is concentrated, concentrated, dry agar-agar ester.
9. the rotten preparation method of a kind of fish according to claim 1 and 2, is characterized in that: adding albumen complex enzyme and within 10-100 minute, carrying out incubation in the placement of 20-50 degree scope inside holding after stirring evenly.
10. the rotten preparation method of a kind of fish according to claim 6, is characterized in that: after interpolation albumen complex enzyme, first place 10-20 minute at 35-45 degree scope inside holding, plays catalytic crosslinking effect to allow glutamine transaminage and polyphenol oxidase; Then under 55-65 degree, be incubated 5-20 minute and allow the effect of peroxidase generation catalytic crosslinking, allow the sea cucumber albumen of the rotten albumen of fish in the gruel of mixing fish, sea cucumber elite, the sea cucumber polysaccharide of sea cucumber elite, the selenka of sea cucumber elite molecule in and intermolecular cross-linking and bag by selenka.
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| CN107897763A (en) * | 2017-11-06 | 2018-04-13 | 明光市永言水产(集团)有限公司 | A kind of preparation process of crayfish gruel |
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| CN109077257A (en) * | 2018-09-30 | 2018-12-25 | 大连工业大学 | A kind of 3D printing sea cucumber-minced fillet complex material preparation method and application |
| CN113647578A (en) * | 2021-07-02 | 2021-11-16 | 集美大学 | Method for producing minced fillet product |
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Cited By (4)
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