CN104777303A - Basophilic granulocyte activation and degranulation identification method - Google Patents
Basophilic granulocyte activation and degranulation identification method Download PDFInfo
- Publication number
- CN104777303A CN104777303A CN201510212435.7A CN201510212435A CN104777303A CN 104777303 A CN104777303 A CN 104777303A CN 201510212435 A CN201510212435 A CN 201510212435A CN 104777303 A CN104777303 A CN 104777303A
- Authority
- CN
- China
- Prior art keywords
- human
- basophilic granulocyte
- mark
- streaming
- ccr3
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000003714 granulocyte Anatomy 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 32
- 230000004913 activation Effects 0.000 title claims abstract description 25
- 101000998120 Homo sapiens Interleukin-3 receptor subunit alpha Proteins 0.000 claims abstract description 59
- 102100033493 Interleukin-3 receptor subunit alpha Human genes 0.000 claims abstract description 59
- 102100024167 C-C chemokine receptor type 3 Human genes 0.000 claims abstract description 53
- 101710149862 C-C chemokine receptor type 3 Proteins 0.000 claims abstract description 53
- 102000006354 HLA-DR Antigens Human genes 0.000 claims abstract description 43
- 108010058597 HLA-DR Antigens Proteins 0.000 claims abstract description 43
- 210000004369 blood Anatomy 0.000 claims abstract description 39
- 238000012360 testing method Methods 0.000 claims abstract description 36
- 102100025222 CD63 antigen Human genes 0.000 claims abstract description 34
- 101000934368 Homo sapiens CD63 antigen Proteins 0.000 claims abstract description 34
- 239000008280 blood Substances 0.000 claims abstract description 29
- 210000004027 cell Anatomy 0.000 claims abstract description 21
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 21
- 239000007850 fluorescent dye Substances 0.000 claims abstract description 20
- 238000001215 fluorescent labelling Methods 0.000 claims abstract description 20
- 239000006228 supernatant Substances 0.000 claims abstract description 15
- 238000000684 flow cytometry Methods 0.000 claims abstract 4
- 239000007788 liquid Substances 0.000 claims description 34
- 238000011068 loading method Methods 0.000 claims description 21
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 claims description 16
- 239000012530 fluid Substances 0.000 claims description 15
- 238000013016 damping Methods 0.000 claims description 14
- 230000012447 hatching Effects 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 14
- 238000002372 labelling Methods 0.000 claims description 9
- 238000005119 centrifugation Methods 0.000 claims description 7
- 239000008363 phosphate buffer Substances 0.000 claims description 7
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 7
- 239000012498 ultrapure water Substances 0.000 claims description 7
- 239000003643 water by type Substances 0.000 claims description 7
- 238000001556 precipitation Methods 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 5
- 101000897042 Homo sapiens Nucleotide pyrophosphatase Proteins 0.000 abstract description 7
- 102100021969 Nucleotide pyrophosphatase Human genes 0.000 abstract description 7
- 238000001514 detection method Methods 0.000 abstract description 6
- 210000005259 peripheral blood Anatomy 0.000 abstract description 2
- 239000011886 peripheral blood Substances 0.000 abstract description 2
- 238000011534 incubation Methods 0.000 abstract 3
- 239000013592 cell lysate Substances 0.000 abstract 1
- 239000013566 allergen Substances 0.000 description 7
- 206010020751 Hypersensitivity Diseases 0.000 description 6
- 238000003745 diagnosis Methods 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 210000003630 histaminocyte Anatomy 0.000 description 5
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 4
- 208000030961 allergic reaction Diseases 0.000 description 4
- 208000035474 group of disease Diseases 0.000 description 4
- 230000001404 mediated effect Effects 0.000 description 4
- 208000003455 anaphylaxis Diseases 0.000 description 3
- 208000006673 asthma Diseases 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 206010002216 Anaphylactoid reaction Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 208000026935 allergic disease Diseases 0.000 description 2
- 230000007815 allergy Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 229960001340 histamine Drugs 0.000 description 2
- 210000003712 lysosome Anatomy 0.000 description 2
- 230000001868 lysosomic effect Effects 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 206010061623 Adverse drug reaction Diseases 0.000 description 1
- 206010002198 Anaphylactic reaction Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010013700 Drug hypersensitivity Diseases 0.000 description 1
- 208000030453 Drug-Related Side Effects and Adverse reaction Diseases 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 208000001718 Immediate Hypersensitivity Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000031662 Noncommunicable disease Diseases 0.000 description 1
- 206010039085 Rhinitis allergic Diseases 0.000 description 1
- 206010045240 Type I hypersensitivity Diseases 0.000 description 1
- 230000002009 allergenic effect Effects 0.000 description 1
- 201000009961 allergic asthma Diseases 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- 201000010105 allergic rhinitis Diseases 0.000 description 1
- 230000036783 anaphylactic response Effects 0.000 description 1
- 208000010216 atopic IgE responsiveness Diseases 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 210000003651 basophil Anatomy 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 201000005311 drug allergy Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 201000010659 intrinsic asthma Diseases 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000035778 pathophysiological process Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000009290 primary effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/24—Immunology or allergic disorders
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (15)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510212435.7A CN104777303B (en) | 2015-04-29 | 2015-04-29 | Basophilic granulocyte activation and degranulation identification method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510212435.7A CN104777303B (en) | 2015-04-29 | 2015-04-29 | Basophilic granulocyte activation and degranulation identification method |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104777303A true CN104777303A (en) | 2015-07-15 |
CN104777303B CN104777303B (en) | 2017-02-01 |
Family
ID=53618900
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510212435.7A Active CN104777303B (en) | 2015-04-29 | 2015-04-29 | Basophilic granulocyte activation and degranulation identification method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104777303B (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105004705A (en) * | 2015-08-07 | 2015-10-28 | 天津中医药大学 | Detection method of medicine injection anaphylactic and anaphylactoid reactions and new application of adopted dye |
CN109387636A (en) * | 2017-08-08 | 2019-02-26 | 欧蒙医学诊断技术有限公司 | Method for examining basicyte to activate |
CN111549091A (en) * | 2020-05-21 | 2020-08-18 | 核工业总医院 | Test method for basophil activation and degranulation |
WO2023046111A1 (en) * | 2021-09-23 | 2023-03-30 | 上海市第一人民医院 | Basophil activation detection method and application thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2037269A1 (en) * | 2007-09-11 | 2009-03-18 | Bühlmann Laboratories AG | Allergy test based on flow cytometric analysis |
WO2010043724A1 (en) * | 2008-10-17 | 2010-04-22 | Mead Johnson & Company | Method for identifying allergenic proteins and peptides |
CN102590492A (en) * | 2012-02-22 | 2012-07-18 | 江苏苏中药业集团股份有限公司 | Method for detecting anaphylactoid reaction of Shengmai liquid preparation |
-
2015
- 2015-04-29 CN CN201510212435.7A patent/CN104777303B/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2037269A1 (en) * | 2007-09-11 | 2009-03-18 | Bühlmann Laboratories AG | Allergy test based on flow cytometric analysis |
WO2010043724A1 (en) * | 2008-10-17 | 2010-04-22 | Mead Johnson & Company | Method for identifying allergenic proteins and peptides |
CN102590492A (en) * | 2012-02-22 | 2012-07-18 | 江苏苏中药业集团股份有限公司 | Method for detecting anaphylactoid reaction of Shengmai liquid preparation |
Non-Patent Citations (3)
Title |
---|
GUILLAUME MONNERET ET AL.: "Monitoring of Basophil Activation Using CD63 and CCR3 in Allergy to Muscle Relaxant Drugs", 《CLINICAL IMMUNOLOGY》 * |
孙林等: "外周血嗜碱粒细胞的定量研究", 《中国医药指南》 * |
张皓月等: "流式细胞术分析嗜碱性粒细胞活化试验在过敏性疾病诊断中的应用", 《中国医药导报》 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105004705A (en) * | 2015-08-07 | 2015-10-28 | 天津中医药大学 | Detection method of medicine injection anaphylactic and anaphylactoid reactions and new application of adopted dye |
CN105004705B (en) * | 2015-08-07 | 2018-05-01 | 天津中医药大学 | The detection method of drug injection allergy anaphylactoid reaction and dyestuff new application used |
CN109387636A (en) * | 2017-08-08 | 2019-02-26 | 欧蒙医学诊断技术有限公司 | Method for examining basicyte to activate |
CN111549091A (en) * | 2020-05-21 | 2020-08-18 | 核工业总医院 | Test method for basophil activation and degranulation |
CN111549091B (en) * | 2020-05-21 | 2023-11-17 | 核工业总医院 | Method for testing activation and degranulation of basophils |
WO2023046111A1 (en) * | 2021-09-23 | 2023-03-30 | 上海市第一人民医院 | Basophil activation detection method and application thereof |
Also Published As
Publication number | Publication date |
---|---|
CN104777303B (en) | 2017-02-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104777303A (en) | Basophilic granulocyte activation and degranulation identification method | |
Jung et al. | Immature platelet fraction: establishment of a reference interval and diagnostic measure for thrombocytopenia | |
Perné et al. | Performance evaluation of the Sysmex XE-5000 hematology analyzer for white blood cell analysis in cerebrospinal fluid | |
WO2021012925A1 (en) | Method for measuring human peripheral blood lymphocytes | |
CN104830986A (en) | Method, apparatus, and system for detecting fetus gene information | |
CN106399510A (en) | Method for analyzing urine exosome miRNA | |
CN104651513A (en) | Gout serum miRNAs biomarkers and method for detecting expression quantity thereof | |
JP2019032317A5 (en) | ||
Tailliar et al. | Urinary peptides as potential non-invasive biomarkers for lupus nephritis: results of the peptidu-LUP study | |
CN104807988A (en) | Specific immunity recognition method for basophilic granulocyte | |
CN203625357U (en) | Circulating tumor cell separation system | |
CN102818890A (en) | Method for detecting autoantibodies of systemic lupus erythematosus patient | |
CN104792687B (en) | A kind of authentication method of basophilic granulocyte sensitization | |
CN107121551B (en) | Biomarker combinations, detection kit and the application of nasopharyngeal carcinoma | |
CN113125757B (en) | Protein biomarker for early pregnancy diagnosis of sows and method for detecting early pregnancy of sows by using protein biomarker | |
CN107102127B (en) | Monokaryon sample marrow source inhibits the detection method of cell in a kind of human peripheral blood | |
Haque et al. | Evaluation of Biochemical marker for the diagnosis of Rheumatoid arthritis | |
CN209450535U (en) | Haemolysis indicates constant volume heparin tube | |
WO2018170875A1 (en) | Pet disease prevention and diagnosis method based on nanometer-scale gene anchoring and microfluidic immunological agglutination technology | |
CN204008664U (en) | For detection of PNH clone's kit | |
CN202710573U (en) | Indoor quality control product reagent kit for transfusion compatibility detection | |
Sareen et al. | Laboratory disease: Case of pseudothrombocytopenia | |
Braga et al. | Proteinuria After 12 Months of Treatment For Lupus Nephritis As Predictor of Long-Term Renal Outcome: A Retrospective Cohort Study | |
CN107340225A (en) | One kind is based on Flow cytometry platelet receptor GPIba extracellular fragment digestion methods | |
CN117074692B (en) | Identification method for tumor cell HER2 antigen expression biological information in cerebrospinal fluid |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
EXSB | Decision made by sipo to initiate substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20160516 Address after: 121004, Fairview District, Taihe District, Jinzhou City, Liaoning Province, D, 35-97 Applicant after: He Shaoheng Address before: 121001 No. five, Section 2, Renmin Street, Guta District, Liaoning, Jinzhou Applicant before: No.1 Hospital Attached To Liaoning Medical College |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20170630 Address after: 121010, 29, Yu Jie street, Taihe District, Liaoning, Jinzhou Patentee after: Liaoning universal source of biomedical science and Technology Development Co., Ltd. Address before: 121004, Fairview District, Taihe District, Jinzhou City, Liaoning Province, D, 35-97 Patentee before: He Shaoheng |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Basophilic granulocyte activation and degranulation identification method Effective date of registration: 20190808 Granted publication date: 20170201 Pledgee: Jinzhou Bank Co., Ltd. Shifulu Branch of Science and Technology Pledgor: Liaoning universal source of biomedical science and Technology Development Co., Ltd. Registration number: Y2019990000043 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20200408 Granted publication date: 20170201 Pledgee: Jinzhou Bank Co., Ltd. Shifulu Branch of Science and Technology Pledgor: LIAONING HUIPUYUAN BIOMEDICAL TECHNOLOGY DEVELOPMENT Co.,Ltd. Registration number: Y2019990000043 |