CN104771482A - Use of melampyrum roseum in preparation of alpha-glucosaccharase inhibitor - Google Patents
Use of melampyrum roseum in preparation of alpha-glucosaccharase inhibitor Download PDFInfo
- Publication number
- CN104771482A CN104771482A CN201510161848.7A CN201510161848A CN104771482A CN 104771482 A CN104771482 A CN 104771482A CN 201510161848 A CN201510161848 A CN 201510161848A CN 104771482 A CN104771482 A CN 104771482A
- Authority
- CN
- China
- Prior art keywords
- alpha
- trailing plants
- glucosaccharase
- mountain
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the field of traditional Chinese medicines, and in particular relates to use of melampyrum roseum in preparation of an alpha-glucosaccharase inhibitor. The alpha-glucosaccharase inhibitor has high alpha-glucosaccharase inhibiting capability; and the alpha-glucosaccharase inhibitor can be further developed as a food, a health-care food or a medicament for treating and assisting to treat type II diabetes.
Description
Technical field
The present invention relates to the field of Chinese medicines, particularly alpha-glucosidase inhibitor.
Background technology
Due to the quickening of modern life rhythm and improving constantly of living standard, the sickness rate of various disease is caused constantly to rise, as obesity, heart disease and diabetes etc.Show according to investigations, the whole world will have 4.38 hundred million person-times to suffer from diabetes to the year two thousand thirty in the country that middle low-level is taken in, and wherein the overwhelming majority belongs to type Ⅱdiabetes mellitus.Diabetes cause because of metabolism disorder, it is characterized in that the abnormal rising of post-prandial glycemia.Therefore control postprandial hyperglycemia, just seem particular importance in prevention or treatment diabetes mellitus.
Alpha-glucosidase is positioned on intestinal villus film, can by carbohydrate degradation, thus post-prandial glycemia be raised, and alpha-glucosidase inhibitor can slow down the degraded of carbohydrate competitively and be absorbed.Existing alpha-glucosidase inhibitor such as acarbose and Fu Gelie polysaccharide are applied to the clinical treatment of diabetes as oral antidiabetic drug at present, it effectively can control level of postprandial blood sugar, but this kind of alpha-glucosidase inhibitor is minority after all in commercially available, and it is mostly containing glycosyl, synthesis step also and loaded down with trivial details, in addition, at clinicing aspect, it has certain side effect as caused flatulence and diarrhoea etc.Therefore, increasing scholar starts to favor the alpha-glucosaccharase enzyme level in finding natural product replacement synthetic from food or medicinal plants, to ensure its security row and effectiveness.
We, in nearly hundred kind of plant screening active ingredients processes of Changbaishan area, have found several plant with stronger alpha-glucosaccharase enzyme inhibition activity, have wherein just comprised mountain trailing plants.
Scrophulariaceae mountain trailing plants
melampyrum roseummaxim., be used as medicine with herb, root.Be distributed in the ground such as northeast, East China and Hebei, Shanxi, Shaanxi, Gansu, Henan, Hubei, Hunan.Be born in hillside, sparse woods, shrubbery and high thick grass.The function that its herb has heat-clearing and toxic substances removing is recorded, for carbuncle sore tumefacting virus in " Xinhua's book on Chinese herbal medicine outline ".Root: make tea, has refrigerant effect.The bibliographical information of the chemical composition of current pass Yushan Hill trailing plants, biological activity, product development is less, and the research of closing the alpha-glucosaccharase enzyme inhibition activity of Yushan Hill trailing plants is blank especially.
Summary of the invention
Based on above reason, an object of the present invention is to provide the new opplication of mountain trailing plants or its extract, and this application solves the suppression problem of alpha-glucosidase.
To achieve these goals, technical scheme of the present invention is:
Mountain trailing plants or mountain trailing plants flower extract are used for the application of Inhibiting α-glucosidase.
Two of object of the present invention is to provide the another kind application of mountain trailing plants or mountain trailing plants flower extract, and this is applied as treatment type Ⅱdiabetes mellitus and provides a kind of new food, health food, medicine.
To achieve these goals, technical scheme of the present invention is:
Mountain trailing plants or mountain trailing plants flower extract are for the preparation of the application of the food for the treatment of type Ⅱdiabetes mellitus, health food, medicine.
Described mountain trailing plants or mountain trailing plants flower extract, for the preparation of the application of the food of alpha-glucosidase inhibitor treatment type Ⅱdiabetes mellitus, health food, medicine, can be prepared by following method:
1. get mountain trailing plants, dry, pulverize, make the pharmaceutically acceptable dosage form such as tablet, capsule, powder, pill.
2. get mountain trailing plants, extract, be concentrated into dry, obtain mountain trailing plants flower extract with water or ethanol equal solvent, with this extract for raw material, additional pharmacy acceptable adjuvant, makes the pharmaceutically acceptable dosage form such as tablet, capsule, powder, pill, soft capsule.
3. get mountain trailing plants, with the extraction such as water, ethanol, be concentrated into dry, obtain mountain trailing plants flower extract, with this extract for raw material makes the food such as liquid beverage, solid beverage, health food acceptable form.
Detailed description of the invention
In order to more detailed description the present invention, provide and followingly prepare example.But scope of the present invention is not limited thereto.
Embodiment 1: the application of mountain trailing plants Inhibiting α-glucosidase
1. the preparation of mountain trailing plants alpha-glucosidase inhibitor
Collect fresh mountain trailing plants, dry in the shade, with 75% ethanol ultrasonic extraction three times, each 30 minutes, filter, merging filtrate, evaporate to dryness, pulverize, make hard capsule preparation.
2. mountain trailing plants alpha-glucosaccharase enzyme inhibition activity research
2.1 materials and methods
2.1.1 experiment material
Raw material: mountain trailing plants alpha-glucosidase inhibitor.
Reagent: acarbose; Alpha-glucosidase (≤5000u/g); The reagent such as DMSO (dimethyl sulfoxide), sodium hydrogen phosphate, potassium dihydrogen phosphate are analytical pure.
Instrument: electronic balance, vortex mixed instrument, numerical control ultrasonic cleaner, automatic microplate reader.
2.1.2 experimental technique
2.1.2.1 the preparation of need testing solution
Get mountain trailing plants alpha-glucosidase inhibitor content 4 mg, accurately weighed, add 200 μ L DMSO (volume fraction is 20 %), make it dissolve, its concentration is 20 mgmL
-1, for subsequent use.
2.1.2.2 alpha-glucosaccharase enzyme inhibition activity measures
Sucrose is decomposed into glucose under the effect of alpha-glucosidase, by the growing amount of glucose in determination of glucose oxidase reaction system, calculates test sample to the inhibitory action of alpha-glucosidase activity.Get need testing solution, be diluted to series concentration.During test, get the need testing solution 10 μ L of series concentration respectively, add 0.5 umL
-1alpha-glucosaccharase enzymatic solution 10 μ L, then add 25 mmolL
-1sucrose 10 μ L, 37 DEG C of incubations 15 minutes, this reacted on 96 well culture plates and completes, and adopted glucose oxidase method, by microplate reader, measured absorbance (A), using the growing amount of glucose as index calculate suppression ratio under 490 nm wavelength; Choose acarbose as positive control drug, set blank simultaneously, negative control and sample controls.Inhibition of enzyme activity rate (%) is calculated as follows:
Inhibition of enzyme activity rate %=[
a negative-(
a sample-
a sample controls)]/(
a negative-
a blank) × 100%
In formula:
a negativefor not adding the absorbance of sample solution reaction system;
a samplefor adding the absorbance of sample solution reaction system;
a sample controlsfor adding sample solution but not adding the absorbance of alpha-glucosidase reaction system;
a blankfor the absorbance of buffer solution.
The alpha-glucosaccharase enzyme inhibition activity IC of sample
50represent, IC
50the concentration of need testing solution during for reaching 50% to alpha-glucosaccharase enzyme inhibition rate.
2.1.2.3 data statistic analysis
Use ORIGIN computed in software IC
50value, IC
50be worth less, represent that it is stronger to the rejection ability of alpha-glucosidase.
2.2 results and analysis
By above experiment, the mountain trailing plants alpha-glucosidase inhibitor content of mensuration is to the IC of alpha-glucosaccharase enzyme inhibition rate
50be 0.17 mgmL
-1, and under the same terms acarbose to the IC of alpha-glucosaccharase enzyme inhibition rate
50be only 1.35 mgmL
-1, the mountain rejection ability of trailing plants alpha-glucosidase inhibitor to alpha-glucosidase is nearly 8 times of acarbose.
2.3 conclusion
Mountain trailing plants alpha-glucosidase inhibitor has stronger rejection ability to alpha-glucosidase, as natural plants, with mountain trailing plants for raw material can develop food, health food or the medicine being used for the treatment of type Ⅱdiabetes mellitus further, it is to the far super acarbose of the rejection ability of alpha-glucosidase.
Embodiment 2: the preparation of mountain trailing plants flower capsule
Collect fresh mountain trailing plants, dry in the shade, with ethanol ultrasonic extraction three times, each 30 minutes, filter, merging filtrate, evaporate to dryness, redissolve as the ultrasonic suspendible of dissolving using soybean oil, make soft capsule.
Embodiment 3: the preparation of mountain trailing plants teabag
Collect fresh mountain trailing plants, dry in the shade, pulverize, cross 40 mesh sieves, 24 mesh sieve screenings, Burdick lamp sterilizes, machinery pack, packaging, spray printing date, plastic packaging, warehouse-in.
Claims (1)
1. mountain trailing plants is preparing the purposes in alpha-glucosidase inhibitor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510161848.7A CN104771482A (en) | 2015-04-08 | 2015-04-08 | Use of melampyrum roseum in preparation of alpha-glucosaccharase inhibitor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510161848.7A CN104771482A (en) | 2015-04-08 | 2015-04-08 | Use of melampyrum roseum in preparation of alpha-glucosaccharase inhibitor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104771482A true CN104771482A (en) | 2015-07-15 |
Family
ID=53613465
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510161848.7A Pending CN104771482A (en) | 2015-04-08 | 2015-04-08 | Use of melampyrum roseum in preparation of alpha-glucosaccharase inhibitor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104771482A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107468678A (en) * | 2017-08-29 | 2017-12-15 | 江苏食品药品职业技术学院 | Application of the D-CARVONE as α glucosidase inhibitors in terms of antihypelipidemic foodstuff, medicine and health products are prepared |
-
2015
- 2015-04-08 CN CN201510161848.7A patent/CN104771482A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| DU ZY,ET AL: "a-Glucosidase inhibition of natural curcuminoids and curcumin analogs", 《EUR J MED CHEM》 * |
| 于顺利编著: "《城市周边野花草识别》", 31 July 2014, 机械工业出版社 * |
| 庞博等: "糖尿病清热解毒治法探讨", 《中华中医药杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107468678A (en) * | 2017-08-29 | 2017-12-15 | 江苏食品药品职业技术学院 | Application of the D-CARVONE as α glucosidase inhibitors in terms of antihypelipidemic foodstuff, medicine and health products are prepared |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20120309710A1 (en) | Compound sea cucumber preparation and manufacturing method thereof | |
| Kochhar et al. | Effect of supplementation of traditional medicinal plants on blood glucose in non–insulin-dependent diabetics: A pilot study | |
| Geethalakshmi et al. | α-Amylase inhibitory activity of Trianthema decandra L | |
| CN104546957B (en) | A kind of water chestnut shell polyphenol extract and preparation method and application | |
| Edem et al. | Effect of aqueous extracts of alligator pear seed (Persea americana mill) on blood glucose and histopathology of pancreas in alloxan-induced diabetic rats. | |
| CN101474313B (en) | Application of theabrownin in pharmacy | |
| CN104922173B (en) | The application of le dish extract inhibition alpha-glucosidase | |
| CN104906199A (en) | Buckwheat husk flavonoid extractive and applications as DPP4 inhibitor | |
| Selfayan et al. | Inhibitory effect of Capparis spinosa extract on pancreatic alpha-amylase activity | |
| Singh et al. | Screening for anti-diabetic activity of the ethanolic extract of Bryonia Alba roots | |
| CN102293802B (en) | Medicinal composition for treating cardiovascular diseases and preparation method thereof | |
| CN102114170B (en) | Traditional Chinese medicine composition for preventing and treating myocardial ischemia reperfusion injury and preparation method thereof | |
| CN104771482A (en) | Use of melampyrum roseum in preparation of alpha-glucosaccharase inhibitor | |
| Indriawati | The Hepatoprotective Capacity of Steeping kersen Leaves (Muntingia calabura L.) on Diabetic Rat. Electron J Gen Med. 2020; 17 (5): em225 | |
| CN103153323B (en) | Memory and learning capacity enhancing compositions | |
| CN104000877A (en) | Blood glucose reducing composition and application thereof | |
| CN104784237A (en) | Application of Italian cocklebur in preparation of alpha-glucosidase inhibitor | |
| CN104771481A (en) | Application of ptheirospermum japonicum in preparing alpha-glucosidase inhibitor | |
| CN105770098A (en) | Blood pressure reducing composition and application thereof | |
| CN101474312A (en) | Application of theaflavin in pharmacy | |
| RICHARD et al. | Ameliorative potentials of methanolic leaf extract of nephrolepis undulate in streptozotocin-induced diabetic wistar rats | |
| NL2027132B1 (en) | Use of a composition in preparing a product that inhibits α-glucosidase activity | |
| CN110279730A (en) | A kind of vepal cwrrant Fischer seed extract and its application in preparation reduction postprandial blood sugar drug | |
| CN113181229B (en) | Application of cabbage type rape-isatis tinctoria G monomer addition system in inhibiting novel coronavirus SARS-CoV-2 | |
| CN107669708A (en) | A kind of kelp base hypoglycemic product and preparation method thereof, application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150715 |