CN104770287A - Method for improving paddy blast resistance by using mid-early 39 - Google Patents

Method for improving paddy blast resistance by using mid-early 39 Download PDF

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CN104770287A
CN104770287A CN201510175308.4A CN201510175308A CN104770287A CN 104770287 A CN104770287 A CN 104770287A CN 201510175308 A CN201510175308 A CN 201510175308A CN 104770287 A CN104770287 A CN 104770287A
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blast resistance
rice
pib
pid3
rice blast
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CN104770287B (en
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杨长登
曾宇翔
季芝娟
梁燕
夏令治
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China National Rice Research Institute
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China National Rice Research Institute
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Abstract

The invention discloses a method for improving paddy blast resistance by using mid-early 39. The method comprises the steps of crossing a paddy variety mid-early 39 carrying five blast resistance genes Pi-d2, Pid3, Pi5, Pib and Pi9 with a paddy material with blast resistance to be improved without carrying the five blast resistance genes, performing multiple times of back crossing by using the paddy material with blast resistance to be improved as a recurrent parent, performing back crossing on single plants simultaneously carrying the five blast resistance genes selected by using specific functional markers in each back crossing generation and the recurrent parent, and obtaining an improved paddy offspring material simultaneously gathering the five blast resistance genes of the mid-early 39 and having agronomic traits similar to those of the paddy material with blast resistance to be improved after multiple times of back crossing and selfing. The method fulfills the purpose of improving the blast resistance by gathering the five blast resistance genes of the mid-early 39.

Description

A kind of method of improvement morning 39 Rice Resistance To Rice Blasts in utilization
Technical field
The present invention relates to 5 rice blast resistance genes in a kind of utilization early entrained by 39 to improve the rice blast resistance of rice material, improve with seed selection rice blast resistance and economical character to wait to improve the similar method of rice material.
Background technology
Paddy rice is the important cereal crops of China.Rice blast is one of most serious plant disease of hazard rice, is considered to for many years first of the large disease of paddy rice three always.The rice varieties cultivating blast resisting is the most economical effective approach of the control rice blast that breeding circle is generally acknowledged always.Utilizing multiple rice blast resistance gene and being aggregated in same kind by different resistant genes is the prerequisite and the basis that make this kind obtain durable resistance.The conventional Indica Rice Cultivars that Zhong Zao39Shi China Paddy Rice Inst cultivates, was authorized in 2009 by Zhejiang Province, and 2012 by country's authorization.Regarded as by the Ministry of Agriculture and morning 39 in super hybridization rice there is good rice blast resistance, early 39 carry 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 during the present inventor is found by molecular Biological Detection, therefore, early 39 can be used as the donor parents of rice blast resistance gene to improve the rice blast resistance of rice material.In this context, the present invention proposes a kind of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 carried middle morning 39 to import to the paddy rice of not carrying these 5 resistant genes simultaneously and treat in improved materials, to obtain the breeding method of the rice material of rice blast resistance improvement.
Summary of the invention
What the present invention adopted is 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 existed in morning 39 in utilizing specific function mark to be optionally polymerized, and improves and economical character and the breeding method treating the rice material that improved materials is basically identical with seed selection rice blast resistance in conjunction with conventional breeding means.The present invention mainly contains two advantages: early 39 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 carried during breeding material that (A) finally obtains is polymerized simultaneously; (B) make the final breeding material obtained and treat that the economical character of improved materials is consistent substantially, being only improved on rice blast resistance.
The present invention includes following steps:
(1) rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 is treated improvement rice material and carry to hybridize morning 39 in 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9, obtain hybrid F 1generation;
(2) the hybrid F that step (1) obtains is planted 1generation, and by hybrid F 1the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 that generation and step (1) describe waits that improveing rice material backcrosses, and obtains BC 1f 1for seed;
(3) BC that step (2) obtains is planted 1f 1for seed, and utilize the specific function Marker selection detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to carry the BC of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously 1f 1the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 described for individual plant and step (1) waits that improveing rice material backcrosses, and obtains BC 2f 1for seed;
(4) BC that step (3) obtains is planted 2f 1for seed, and utilize the specific function Marker selection detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to carry the BC of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously 2f 1the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 described for individual plant and step (1) waits that improveing rice material backcrosses, and obtains BC 3f 1for seed;
(5) BC that step (4) obtains is planted 3f 1for seed, and utilize the specific function Marker selection detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to carry the BC of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously 3f 1the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 described for individual plant and step (1) waits that improveing rice material backcrosses, and obtains BC 4f 1for seed;
(6) BC that step (5) obtains is planted 4f 1for seed, and utilize the specific function Marker selection detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to carry the BC of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously 4f 1for individual plant, the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 described with step (1) waits that improveing rice material carries out continuous backcross, each backcross generations all selects to carry the individual plant of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously and recurrent parent backcrosses, until obtain satisfied backcross progeny BC mf 1till (m>=5);
(7) BC that step (6) obtains is planted mf 1(m>=5), for individual plant, utilize the specific function Marker selection detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to carry the BC of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously mf 1(m>=5), for individual plant selfing, selfing algebraically needs to determine according to breeding, obtains the BC that economical character is stable mf n(m>=5, n>=2) are for material;
(8) the stable BC of step (7) economical character that obtains is planted mf n(m>=5, n>=2), for material, utilize the specific function Marker selection detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to carry the BC of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously mf n(m>=5, n>=2) for individual plant, obtain rice blast resistance improvement and the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 that economical character and step (1) describe wait to improve the similar improved materials of rice material.
Further, in described step (1), the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 treats that improvement rice material can be long-grained nonglutinous rice, also can be japonica rice, can be restorer, also can be the breeding material that maintainer etc. is different.
Further, described step (2), step (3), step (4), step (5), step (6) utilize the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to treat, and the object that improvement rice material carries out continuous backcross as recurrent parent is, makes the economical character of the final rice material obtained and the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 wait to improve rice material similar.
Further, in described step (3), step (4), step (5), step (6), step (7), step (8), the individual plant utilizing the specific function Marker selection detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously to carry 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 carries out backcrossing or the object of selfing is, ensures that 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 exist all the time in improved progeny.
Further, in described step (3), step (4), step (5), step (6), step (7), step (8), the specific function mark detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 can carry out self-developing according to the openly DNA sequence dna of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9.
Embodiment
Be described in detail with specific embodiment: in utilization in early 39 improvement the rice blast resistance of No. 1, group (middle group No. 1 conventional Indica Rice Cultivars being China Paddy Rice Inst and cultivating, in 1999 by Zhejiang Province's variety certification; The present inventor utilizes No. 1, group in specific functional label detection display not carry 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9, and carry 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 middle morning 39), concrete implementation step is as follows:
1. middle morning 39 is hybridized with middle group No. 1, obtain hybrid F 1for 19, seed (No. 1 can be organized in female parent and do male parent and hybridize with doing middle morning 39, also can to make in female parent of middle group No. 1 and early 39 do male parent and hybridize);
2. kind plant hybrid F 1, by hybrid F 1backcross for individual plant and middle group No. 1, obtain BC 1f 1for 32, seed; Plantation BC 1f 1for seed, utilize 5 specific functional labels to BC 1f1 generation individual plant detects, and selects the individual plant simultaneously carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to backcross with middle group No. 1, acquisition BC 2f 1for 41, seed;
3. kind plant BC 2f 1for individual plant, utilize 5 specific functional labels to BC 2f 1detect for individual plant, select the individual plant simultaneously carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to backcross with middle group No. 1, acquisition BC 3f 1for 133, seed;
4. kind plant BC 3f1 generation individual plant, utilizes 5 specific functional labels to BC 3f 1detect for individual plant, select the individual plant simultaneously carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to backcross with middle group No. 1, acquisition BC 4f 1for 75, seed;
5. kind plant BC 4f 1for individual plant, utilize 5 specific functional labels to BC 4f 1detect for individual plant, select the individual plant simultaneously carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to backcross with middle group No. 1, acquisition BC 5f 1for 113, seed;
6. kind plant BC 5f 1for individual plant, utilize 5 specific functional labels to BC 4f 1detect for individual plant, select the individual plant simultaneously carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to backcross with middle group No. 1, acquisition BC 6f 1for 216, seed;
7. kind plant BC 6f1 generation individual plant, utilizes 5 specific functional labels to BC 4f 1detect for individual plant, select individual plant totally 36 strains of simultaneously carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9; 36 BC of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 will be carried simultaneously 6f 1gather in the crops respectively for individual plant, every individual plant receives 1 fringe, obtains 36 BC 6f 2for strain;
8. kind plant 36 BC 6f 2for strain, the strain of every strain kind 6, time ripe, every strain receives 1 fringe at random, obtains 36 BC 6f 3for strain;
9. kind plant 36 BC 6f 3for strain, the strain of every strain kind 6, time ripe, every strain receives 1 fringe at random, obtains 36 BC 6f 4for strain;
10. kind plant 36 BC 6f 4for strain, the strain of every strain kind 6, every strain is selected 1 individual plant to extract leaf DNA and is utilized 5 specific functional labels to detect, find that wherein 13 strains carry 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9, these 13 BC simultaneously 6f 4for strain for having imported 5 rice blast resistance genes, and the final improvement strain that economical character is similar with middle group No. 1.
The development approach detecting the specific functional label whether 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 exist is the DNA sequence dna self-developing of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 that basis has been reported.
Detecting the method whether a paddy rice individual plant carry 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 is simultaneously: the leaf DNA utilizing 5 independently developed corresponding specific function marks these individual plants of pcr amplification respectively, if 5 corresponding specific function marks can amplify band simultaneously, then this individual plant carries 5 rice blast resistance genes simultaneously.
The experimental techniques such as the pcr amplification that the present invention relates to and the electrophoresis detection of amplified production are conventional experimental technique, therefore here repeat no more.

Claims (5)

1. a method for improvement morning 39 Rice Resistance To Rice Blasts in utilizing, is characterized in that, comprise the following steps:
(1) rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 is treated improvement rice material and carry to hybridize morning 39 in 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9, obtain hybrid F 1generation;
(2) the hybrid F that step (1) obtains is planted 1generation, and by hybrid F 1the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 that generation and step (1) describe waits that improveing rice material backcrosses, and obtains BC 1f 1for seed;
(3) BC that step (2) obtains is planted 1f 1for seed, and utilize the specific function Marker selection detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to carry the BC of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously 1f 1the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 described for individual plant and step (1) waits that improveing rice material backcrosses, and obtains BC 2f 1for seed;
(4) BC that step (3) obtains is planted 2f 1for seed, and utilize the specific function Marker selection detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to carry the BC of 5 rice blast resistance genes such as Pi-d2Pid3, Pi5, Pib, Pi9 simultaneously 2f 1the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 described for individual plant and step (1) waits that improveing rice material backcrosses, and obtains BC 3f 1for seed;
(5) BC that step (4) obtains is planted 3f 1for seed, and utilize the specific function Marker selection detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to carry the BC of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously 3f 1the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 described for individual plant and step (1) waits that improveing rice material backcrosses, and obtains BC 4f 1for seed;
(6) BC that step (5) obtains is planted 4f 1for seed, and utilize the specific function Marker selection detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to carry the BC of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously 4f 1for individual plant, the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 described with step (1) waits that improveing rice material carries out continuous backcross, each backcross generations all selects to carry the individual plant of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously and recurrent parent backcrosses, until obtain satisfied backcross progeny BC mf 1till (m>=5);
(7) BC that step (6) obtains is planted mf 1(m>=5), for individual plant, utilize the specific function Marker selection detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to carry the BC of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously mf 1(m>=5), for individual plant selfing, selfing algebraically needs to determine according to breeding, obtains the BC that economical character is stable mf n(m>=5, n>=2) are for material;
(8) the stable BC of step (7) economical character that obtains is planted mf n(m>=5, n>=2), for material, utilize the specific function Marker selection detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 to carry the BC of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 simultaneously mf n(m>=5, n>=2) for individual plant, obtain rice blast resistance improvement and the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 that economical character and step (1) describe wait to improve the similar improved materials of rice material.
2. in utilization according to claim 1, morning 39 improves the method for Rice Resistance To Rice Blasts, it is characterized in that, in described step (1), the rice blast resistance not carrying 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 treats that improvement rice material can be long-grained nonglutinous rice, also can be japonica rice, can be restorer, also can be the breeding material that maintainer etc. is different.
3. in utilization according to claim 1, morning 39 improves the method for Rice Resistance To Rice Blasts, it is characterized in that, described step (2), step (3), step (4), step (5), step (6) utilizes and does not carry Pi-d2, Pid3, Pi5, Pib, the rice blast resistance of 5 rice blast resistance genes such as Pi9 treats that the object that improvement rice material carries out continuous backcross as recurrent parent is, make the economical character of the final rice material obtained and do not carry Pi-d2, Pid3, Pi5, Pib, the rice blast resistance of 5 rice blast resistance genes such as Pi9 treats that improvement rice material is similar.
4. in utilization according to claim 1, morning 39 improves the method for Rice Resistance To Rice Blasts, it is characterized in that, described step (3), step (4), step (5), step (6), step (7), in step (8), utilize and detect Pi-d2, Pid3, Pi5, Pib, the specific function Marker selection of 5 rice blast resistance genes such as Pi9 carries Pi-d2 simultaneously, Pid3, Pi5, Pib, the individual plant of 5 rice blast resistance genes such as Pi9 carries out backcrossing or the object of selfing is, ensure Pi-d2, Pid3, Pi5, Pib, 5 rice blast resistance genes such as Pi9 exist all the time in improved progeny.
5. in utilization according to claim 1, morning 39 improves the method for Rice Resistance To Rice Blasts, it is characterized in that, in described step (3), step (4), step (5), step (6), step (7), step (8), the specific function mark detecting 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9 carries out self-developing according to the openly DNA sequence dna of 5 rice blast resistance genes such as Pi-d2, Pid3, Pi5, Pib, Pi9.
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Cited By (8)

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CN105830912A (en) * 2016-05-10 2016-08-10 江苏丘陵地区镇江农业科学研究所 Breeding method for rice with high yield and durable neck blast resistance
CN106489717A (en) * 2016-09-28 2017-03-15 四川省农业科学院生物技术核技术研究所 A kind of Rice Resistance To Rice Blast orderly improvement method
CN106613904A (en) * 2016-11-17 2017-05-10 福建农林大学 Method for breeding lasting disease-resistant hybrid rice mixing line
CN106755475A (en) * 2017-01-17 2017-05-31 中国水稻研究所 A kind of method of early 39 rice blast resistance genes in identification super early rice
CN109924121A (en) * 2018-12-11 2019-06-25 华智水稻生物技术有限公司 A method of improvement Rice Resistance To Rice Blast
CN109924120A (en) * 2018-12-11 2019-06-25 华智水稻生物技术有限公司 A method of improvement Rice Resistance To Rice Blast and bacterial leaf spot resistance
CN109924119A (en) * 2018-12-11 2019-06-25 华智水稻生物技术有限公司 A method of improvement bacterial blight of rice and Brown Planthopper Resistance
CN110178719A (en) * 2019-03-22 2019-08-30 华智水稻生物技术有限公司 A method of improvement Rice Resistance To Rice Blast and bacterial leaf spot resistance

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105830912A (en) * 2016-05-10 2016-08-10 江苏丘陵地区镇江农业科学研究所 Breeding method for rice with high yield and durable neck blast resistance
CN106489717A (en) * 2016-09-28 2017-03-15 四川省农业科学院生物技术核技术研究所 A kind of Rice Resistance To Rice Blast orderly improvement method
CN106613904A (en) * 2016-11-17 2017-05-10 福建农林大学 Method for breeding lasting disease-resistant hybrid rice mixing line
CN106755475A (en) * 2017-01-17 2017-05-31 中国水稻研究所 A kind of method of early 39 rice blast resistance genes in identification super early rice
CN109924121A (en) * 2018-12-11 2019-06-25 华智水稻生物技术有限公司 A method of improvement Rice Resistance To Rice Blast
CN109924120A (en) * 2018-12-11 2019-06-25 华智水稻生物技术有限公司 A method of improvement Rice Resistance To Rice Blast and bacterial leaf spot resistance
CN109924119A (en) * 2018-12-11 2019-06-25 华智水稻生物技术有限公司 A method of improvement bacterial blight of rice and Brown Planthopper Resistance
CN109924121B (en) * 2018-12-11 2021-05-28 华智生物技术有限公司 Method for improving rice blast resistance of rice
CN109924120B (en) * 2018-12-11 2021-05-28 华智生物技术有限公司 Method for improving rice blast resistance and bacterial leaf blight resistance of rice
CN110178719A (en) * 2019-03-22 2019-08-30 华智水稻生物技术有限公司 A method of improvement Rice Resistance To Rice Blast and bacterial leaf spot resistance

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