CN104758748B - A kind of Chinese medicine of adjuvant for chemotherapy of tumour - Google Patents
A kind of Chinese medicine of adjuvant for chemotherapy of tumour Download PDFInfo
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Abstract
The present invention relates to the Chinese medicine of adjuvant for chemotherapy of tumour, can effectively solve the problems, such as the immunologic hypofunction that tumor patient generally occurs after chemotherapy, its technical scheme solved is made up of the material medicine of following parts by weight meter：5 30 parts of 10 25 parts of the bighead atractylodes rhizome, 10 30 parts of Poria cocos, 10 25 parts of the membrane of a chicken's gizzard, 5 20 parts of the tuber of dwarf lilyturf, 10 20 parts of radix polygonati officinalis, 5 20 parts of scabrousstem greenbrier rhizome, 3 15 parts of Selaginella tamariscina, 10 20 parts of rhizoma nelumbinis, 5 20 parts of spina gleditsiae, 10 30 parts of pilosella-leaved drymoglossum herb and botrychiam ternatum, the bighead atractylodes rhizome of the present invention, Poria cocos, the membrane of a chicken's gizzard are strengthened the body resistance to consolidate the constitution, the tuber of dwarf lilyturf, radix polygonati officinalis support clearly lung stomach, scabrousstem greenbrier rhizome, Selaginella tamariscina, rhizoma nelumbinis activate blood circulation and disperse blood clots, spina gleditsiae, pilosella-leaved drymoglossum herb, botrychiam ternatum detoxifying and descending turbid, in terms of patients undergoing chemotherapy immunity of organisms is improved, curative effect is affirmed, is the innovation on the medicine of adjuvant for chemotherapy of tumour.
Description
Technical field
The present invention relates to field of medicaments, particularly a kind of Chinese medicine of adjuvant for chemotherapy of tumour.
Background technology
Malignant tumour is the most common disease of today's society, is related to each system of human body, and serious threat the health of people.
The cancer of early stage generally selects operative treatment, but postoperative the problem of still suffering from recurrence and transfer.And the master of middle and advanced stage cancer
It is still based on chemotherapy at present to want treatment method.Although chemotherapy can suppress cancer cell to a certain extent, extend patient's
Life, but in chemotherapy process, because chemotherapeutics suppresses immunocyte, body's immunity can be caused to decline.Therefore, it is auxiliary
The problem of helping the improvement of the medicine of chemotherapy of tumors to be current urgent need to resolve.
The content of the invention
For the above situation, to solve the defect of prior art, the purpose of the present invention is just to provide a kind of adjuvant therapy
The Chinese medicine for the treatment of, can effectively solve the problems, such as the immunologic hypofunction that tumor patient generally occurs after chemotherapy.
The technical scheme that the present invention solves is made up of the material medicine of following parts by weight meter：Bighead atractylodes rhizome 10-25 parts, Fu
Siberian cocklebur 10-30 parts, the membrane of a chicken's gizzard 10-25 parts, tuber of dwarf lilyturf 5-20 part, radix polygonati officinalis 10-20 parts, scabrousstem greenbrier rhizome 5-20 parts, Selaginella tamariscina 3-15 parts, rhizoma nelumbinis
10-20 parts, spina gleditsiae 5-20 parts, pilosella-leaved drymoglossum herb 10-30 parts and botrychiam ternatum 5-30 parts, above-mentioned raw materials medicine is mixed, added
Water logging does not have, and soaks 30-60min, and intense fire is fried open, then is cooked by slow fire 30-40min, filtering, obtains first time filtrate, the dregs of a decoction add water
Submergence, intense fire is fried open, then is cooked by slow fire 30-40min, filtering, obtains second of filtrate, merges filtrate twice, mix.
The bighead atractylodes rhizome of the present invention, Poria cocos, the membrane of a chicken's gizzard are strengthened the body resistance to consolidate the constitution, and the tuber of dwarf lilyturf, radix polygonati officinalis support clearly lung stomach, scabrousstem greenbrier rhizome, Selaginella tamariscina, rhizoma nelumbinis promoting blood circulation
Change and become silted up, spina gleditsiae, pilosella-leaved drymoglossum herb, botrychiam ternatum detoxifying and descending turbid, in terms of patients undergoing chemotherapy immunity of organisms is improved, curative effect affirmative, is auxiliary
The innovation helped on the medicine of chemotherapy of tumors.
Embodiment
The embodiment of the present invention is described in further detail with reference to embodiments.
Embodiment 1
The present invention in the specific implementation, can be made up of the material medicine of following parts by weight meter：11 parts of the bighead atractylodes rhizome, Poria cocos 29
Part, 11 parts of the membrane of a chicken's gizzard, 19 parts of the tuber of dwarf lilyturf, 11 parts of radix polygonati officinalis, 19 parts of scabrousstem greenbrier rhizome, 4 parts of Selaginella tamariscina, 19 parts of rhizoma nelumbinis, 6 parts of spina gleditsiae, pilosella-leaved drymoglossum herb
29 parts and 6 parts of botrychiam ternatum, above-mentioned raw materials medicine is mixed, added water to submerge, and soaks 30-60min, and intense fire is fried open, then text
Fire decocts 30-40min, filtering, obtains first time filtrate, and the dregs of a decoction add water submergence, and intense fire is fried open, then is cooked by slow fire 30-40min,
Filtering, second of filtrate is obtained, merge filtrate twice, mix.
Embodiment 2
The present invention in the specific implementation, can also be made up of the material medicine of following parts by weight meter：17 parts of the bighead atractylodes rhizome, Poria cocos 20
Part, 18 parts of the membrane of a chicken's gizzard, 12 parts of the tuber of dwarf lilyturf, 15 parts of radix polygonati officinalis, 13 parts of scabrousstem greenbrier rhizome, 9 parts of Selaginella tamariscina, 15 parts of rhizoma nelumbinis, 13 parts of spina gleditsiae, pilosella-leaved drymoglossum herb
20 parts and 17 parts of botrychiam ternatum, above-mentioned raw materials medicine is mixed, added water to submerge, and soaks 30-60min, and intense fire is fried open, then text
Fire decocts 30-40min, filtering, obtains first time filtrate, and the dregs of a decoction add water submergence, and intense fire is fried open, then is cooked by slow fire 30-40min,
Filtering, second of filtrate is obtained, merge filtrate twice, mix.
Embodiment 3
The present invention in the specific implementation, can also be made up of the material medicine of following parts by weight meter：24 parts of the bighead atractylodes rhizome, Poria cocos 11
Part, 24 parts of the membrane of a chicken's gizzard, 6 parts of the tuber of dwarf lilyturf, 19 parts of radix polygonati officinalis, 6 parts of scabrousstem greenbrier rhizome, 14 parts of Selaginella tamariscina, 11 parts of rhizoma nelumbinis, 19 parts of spina gleditsiae, pilosella-leaved drymoglossum herb
11 parts and 29 parts of botrychiam ternatum, above-mentioned raw materials medicine is mixed, added water to submerge, and soaks 30-60min, and intense fire is fried open, then text
Fire decocts 30-40min, filtering, obtains first time filtrate, and the dregs of a decoction add water submergence, and intense fire is fried open, then is cooked by slow fire 30-40min,
Filtering, second of filtrate is obtained, merge filtrate twice, mix.
Embodiment 4
The present invention in the specific implementation, can also be made up of the material medicine of following parts by weight meter：Bighead atractylodes rhizome 10-17 parts, Fu
Siberian cocklebur 10-20 parts, the membrane of a chicken's gizzard 10-18 parts, tuber of dwarf lilyturf 5-12 part, radix polygonati officinalis 10-15 parts, scabrousstem greenbrier rhizome 5-13 parts, Selaginella tamariscina 3-9 parts, rhizoma nelumbinis 10-
15 parts, spina gleditsiae 5-13 parts, pilosella-leaved drymoglossum herb 10-20 parts and botrychiam ternatum 5-17 parts, above-mentioned raw materials medicine is mixed, adds water logging
Not yet, 30-60min is soaked, intense fire is fried open, then is cooked by slow fire 30-40min, filtering, obtains first time filtrate, and the dregs of a decoction add water submergence,
Intense fire is fried open, then is cooked by slow fire 30-40min, filtering, obtains second of filtrate, merges filtrate twice, mix.
Embodiment 5
The present invention in the specific implementation, can also be made up of the material medicine of following parts by weight meter：Bighead atractylodes rhizome 18-25 parts, Fu
Siberian cocklebur 21-30 parts, the membrane of a chicken's gizzard 19-25 parts, tuber of dwarf lilyturf 13-20 part, radix polygonati officinalis 16-20 parts, scabrousstem greenbrier rhizome 14-20 parts, Selaginella tamariscina 10-15 parts, lotus root
16-20 parts, spina gleditsiae 14-20 parts, pilosella-leaved drymoglossum herb 21-30 parts and botrychiam ternatum 18-30 parts are saved, above-mentioned raw materials medicine is blended in one
Rise, add water to submerge, soak 30-60min, intense fire is fried open, then is cooked by slow fire 30-40min, filtering, obtains first time filtrate, the dregs of a decoction are again
Adding water to submerge, intense fire is fried open, then is cooked by slow fire 30-40min, filtering, obtains second of filtrate, merges filtrate twice, mix.
In instant component：
The bighead atractylodes rhizome：It is bitter, sweet, temperature.Returns spleen, stomach.Strengthening the spleen and replenishing qi, eliminate dampness and have diuretic effect, hidroschesis are antiabortive.For spleen eating less, abdominal distension
Diarrhea, phlegm retention anti-dazzle nervous, oedema, spontaneous perspiration, fetal irritability.Rhizoma Atractylodis Macrocephalae invigorating the spleen, and stomach, it is antiabortive.For spleen eating less, diarrhea loose stool,
Fetal irritability.
Poria cocos：It is sweet, light, put down.The thoughts of returning home, lung, spleen, kidney channel.Clearing damp and promoting diuresis, invigorating the spleen calming heart.It is dizzy for oedema oliguria, phlegm and retained fluid
Throb with fear, spleen eating less, loose stool diarrhea, confused and worried, insomnia of palpitating with fear.
The membrane of a chicken's gizzard：It is sweet, put down.:Returns spleen, stomach, small intestine, bladder warp.Stomach strengthening and digestion promoting, arresting seminal emission.For dyspepsia, vomiting
Rush down dysentery, infantile malnutrition, the enuresis, seminal emission.
The tuber of dwarf lilyturf：It is sweet, slight bitter, it is slightly cold.The thoughts of returning home, lung, stomach.Nourishing Yin and promoting production of body fluid, moistening lung clears away heart-fire.For dryness of the lung dry cough.Consumptive disease cough
Cough, injury thirst, vexed insomnia, Heat Diabetes, dry constipation of intestines；Pharyngeal diphtheria.
Radix polygonati officinalis：It is sweet, it is slightly cold.Attach to the lung and stomach meridians.Nourishing yin to moisten dryness, promote the production of body fluid to quench thirst.Hinder for lung stomach-Yin, cough caused by dryness-heat, dry throat mouth
Yearningly, Heat Diabetes.
Scabrousstem greenbrier rhizome：It is bitterly, it is mild-natured.Curing rheumatism, invigorate blood circulation, removing toxic substances, relieving convulsion ceases wind, anticancer.Lumbago, skelalgia of rheumatism is controlled, children shy
Wind, enteritis, boil, scrofula, cancerous swelling.
Selaginella tamariscina：It is pungent, put down.Return liver, the heart channel of Hang-Shaoyin.Invigorate blood circulation.For closed dysmenorrhea.Wei lump in the abdomen lump in the abdomen, injury from falling down.Selaginella tamariscina carbonizes
The stasis of blood is stopped blooding.For spitting blood, uterine bleeding, have blood in stool, prolapse of the anus.
Rhizoma nelumbinis：It is sweet, puckery, put down.:Return liver, lung, stomach.Hemostasis, dispersing blood stasis.For spitting blood, spitting of blood, hematuria, uterine bleeding.
Spina gleditsiae：It is pungent, temperature.Return liver, stomach.Detumescence eliminating toxic, apocenosis, desinsection.For ulcer from the beginning of or purulence into not bursting；Control outside
Mange leprosy.
Pilosella-leaved drymoglossum herb：It is sweet；It is light；It is micro- cool.Return liver；Lung channel.It is clearing heat and detoxicating；Dispersing swelling and dissipating binds；Hemostasis.Main jaundice with damp-heat pathogen；Hot eyes swells
Bitterly；Otitis media suppurative；Parotitis；Lymphnoditis；Scabies leprosy；Traumatic injury.
Botrychiam ternatum：It is sweet；It is bitter；It is cool；It is slightly cold.Return lung；Liver Channel.It is clearing heat and detoxicating；It is calming the liver to stop the wind；Cough-relieving；Hemostasis；Shade removing.
Main infantile hyperpyrexia is frightened to jerk；Cough with lung heat；Hemoptysis；Pertussis；It is demented；Epilepsy disease；Sore swollen toxin；Scrofula；Venomous snake bite；Hot eyes fire
Eye；Mesh gives birth to ocular.
Above-mentioned component science, reasonable compatibility, support mutually, have strengthen the body resistance to consolidate the constitution, support lung stomach clearly, activate blood circulation and disperse blood clots, solution poisons
The effect of turbid, solve the problems, such as the immunologic hypofunction that tumor patient generally occurs after chemotherapy, good effect, medication peace
Entirely, and through experiment obtained fully proving, relevant experimental data is as follows：
First, clinical trial data of the present invention is as follows：
1st, data and method
1.1 inclusion criteria：
(1) malignant solid tumor made a definite diagnosis through pathology or cytology；(2) general behavior state (kamofsky
Performance sta-tus, KPS) >=50 points of scoring；(3) age >=18 year old；(4) in advance will in respect of completion treatment and research institute
The economic condition asked.
Exclusion standard：The person that do not meet inclusive criteria；To used drug allergy person；Merge intentionally, liver, kidney, endocrine and
The primary disease persons such as hemopoietic system；Other shengbai drugs person is applied.
1.2 general information：
Selection in July, 2007-2009 year July, that accepts for medical treatment met the malignant solid tumor patient into group condition, mainly lung
Cancer, stomach cancer, colon cancer, mammary cancer 1 04, enter and patient is randomly divided into treatment group and control group after group, be shown in Table 1.
The treatment group of table 1 and the comparison of control group clinical data
1.3 therapeutic scheme
Control group：Using chemotherapy regimen:ED-SCLC with EP schemes [(VP-16 etoposides) 100mg, d1~d3,
DDP (cis-platinum) 40mg, d1~d3, repeat within every 3 weeks]；Breast cancer CMF schemes [CTX 600mg, d1, d8, MTX (methopterin)
40mg, d1, d8,5-Fu (fluorouracil) 500mg, d1, d8, repeat within every 4 weeks]；Stomach cancer, intestinal cancer PLF schemes [DDP (cis-platinum)
20mg, d1~d5, CF (folinic acid) 200mg, d1~d5,5-Fu 500mg, d1~d5, are repeated for every 4 weeks].
Treatment group：On the basis of control group treatment, Chinese medicine of the present invention is taken, once in the morning and once at night, is warmly taken, each 200-
300ml.Even served 3 weeks is 1 course for the treatment of.2 groups are evaluated curative effect at the end of 1 course for the treatment of.
1.4 criterion of therapeutical effect 1.4.1 clinical symptoms.Reference《Chinese medicine (new drug) guideline of clinical investigations》In syndrome score
Method is evaluated.Severe 3 is divided, and moderate 2 is divided, slight 1 point, asymptomatic 0 point.Cure:Clinical symptoms integration is 0 after treatment；It is effective:
Integration declines >=2/3 after treatment；Effectively:Integration decline >=1/3 after treatment, but<2/3；It is invalid:Integrate and decline after treatment<1/3.
1.4.2KPS scoring.Evaluated according to WHO the standard of curative effect evaluation.It is effective:KPS scorings improve 20 before relatively being treated after treatment
Divide above person；Effectively:KPS scorings improve 10 points of persons before relatively being treated after treatment；It is invalid:Pretherapy and post-treatment KPS scorings are without changer.
1.4.3 changes of weight.It is effective:More than increased weight 1kg before relatively being treated after treatment；Effectively:Before relatively being treated after treatment
Increased weight is less than more than 1kg；It is invalid:Pretherapy and post-treatment body weight is unchanged.
1.5 statistical procedures are handled using the statistics softwares of SPSS 12.0, and data are usedRepresent, enumeration data
Use x2Examine, measurement data is examined with t, is that difference is statistically significant with P ＜ 0.05.
2nd, result
General symptom improvement situation compares 2.1 two groups of patients before and after treatment.It the results are shown in Table 2.
General symptom improvement situation compares 2 two groups of patients of table before and after treatment
Compared with control group, * P ＜ 0.05, * * P ＜ 0.01
Healing in table 2, do not mean that cancer patient cures, only refer to the scoring event of listd symptom.
The pretherapy and post-treatment KPS scorings situation of 2.2 two groups of patients compares.It the results are shown in Table 3.
The pretherapy and post-treatment KPS scorings situation of 3 two groups of patients of table compares
Compared with control group, * P ＜ 0.05, * * P ＜ 0.01
The pretherapy and post-treatment changes of weight situation of 2.3 two groups of patients compares.It the results are shown in Table 4.
The pretherapy and post-treatment changes of weight situation of 4 two groups of patients of table compares
Compared with control group, * P ＜ 0.05, * * P ＜ 0.01
2.4 influence of the Chinese medicine of the present invention to amynologic index.It the results are shown in Table 5.
Immunologic function compares before and after 5 two groups of Chemotherapy in Patients of table
Note：Each group compares * P before and after treatment<0.05；**P<0.01；The △ P compared with after control group treatment<0.05.
3rd, conclusion
From the above, it is seen that Chinese medicine of the present invention is substantially better than control group, effective in adjuvant for chemotherapy of tumour medicine
Using with actual clinical meaning.
2nd, pharmacological experiment of the present invention is as follows：
Tumor-bearing mice animal model is established, observes Chinese medicine of the present invention to chemotherapy of tumors mouse tumor inhibiting rate, lymphocyte
Propagation, macrophage phagocytic function, NO, TNF-α, blood routine change and the influence of hematopoietic progenitor population generative capacity, with this
The chemotherapy of tumors booster action and its mechanism of Chinese medicine of the present invention are inquired into, is carried out especially by following experiment.
2. materials and methods
2.1 mouse and tumour cell
Male Balb/c mouse (6-8 weeks, weigh 20.0 ± 2.0g) 40.S180 cell lines, by Chinese department of traditional Chinese medicine institute
Pharmacological room of medicine research institute provides.
2.2 medicines and reagent
Medicine is Chinese medicine of the present invention.
Reagent is syklofosfamid ampoule (cyclophosphamide, CTX), and Hengrui Medicine Co., Ltd., Jiangsu Prov. produces
Product.
2.3 animal packets and processing
Experiment is divided into physiological saline group, endoxan group (CTX), Chinese medicine group of the present invention (TCM), Chinese medicine of the present invention and adds ring phosphorus
Acid amides group (TCM+CTX), every group 10.By S180 ascites tumor mouse milky ascites, with normal saline dilution to 2 × 106Individual/
ML and viable count are more than 95%, be inoculated in the right armpit of mouse with 0.2mL subcutaneous, inoculated tumour cell second day, TCM groups and TCM+
CTX groups are according to Chinese medicine preparation 20g/kg gavages, once a day, continuous 7 days, TCM+CTX groups and CTX groups：Syklofosfamid ampoule
By preliminary result 10mg/kg, 1 time a day, totally 7 days.
The preparation of 2.4 splenic lymphocytes
Take out spleen to be put into the culture dish for filling a small amount of PBS liquid, grind spleen with 5mL syringes nook closing member, move into centrifugation
Guan Zhong, suction pipe are blown and beaten for several times, the filtering of 300 mesh nylon cloths, and 260 × g centrifugations 5min collects cell, the cracking of 2mL erythrocyte cracked liquids
Red blood cell, washed 3 times with PBS liquid.It is suspended in the complete mediums of RPMI 1640, experiment is stand-by.
2.5 Testing index
2.5.1 tumour inhibiting rate determines
Experiment terminates, and peels off tumour and spleen, weighs.Tumor control rate=[(physiological saline group average tumor weight-control
Treatment group average tumor weight)]/physiological saline group average tumor weight × 100.
2.5.2 lymphocyte proliferation assay determines
Adjust splenic lymphocytes 5 × 106/ ml is inoculated in 96 porocyte culture plates, separately sets one group and adds ConA (whole mass concentrations
For 5.0 μ g/mL), under the conditions of saturated humidity 5%CO2,37 DEG C, 44h is cultivated, 20 μ LMTT solution (5mg/mL) are added per hole,
Supernatant is abandoned in lucifuge culture 4 hours, suction, and 150 μ L dimethyl sulfoxide (DMSO)s are added per hole, when it is 570nm to determine wavelength on ELIASA
Absorbance.
2.5.3 macrophage phagocytic function determines
The cold phosphate buffers of 10ml rinse mouse peritoneum chamber, collect peritoneal exudation of lymphocytes, 720 × g, 40 DEG C of centrifugations
10 minutes, precooling D-Hanks liquid rinsed once, and 96 orifice plates are per hole 100ul (5 × 106/ ml) prepare cell suspension, cell after 3h
Adherent, cell supernatant is abandoned in suction, and each hole adds 0.075% dimethyl diaminophenazine chloride (100ul/ holes), is incubated 1h, preheating D-Hanks liquid washings
Cell 3 times (pH 7.2), each hole add 100ul lysates, are mixed thoroughly, when it is 540nm to determine wavelength on ELIASA
Absorbance.
2.5.4NO measure
As 2.5.3 descriptions prepare peritoneal exudate cells, according to every hole 1 × 106Be inoculated in 48 orifice plates, macrophage adhesion in
Tissue Culture Plate bottom, 37 DEG C are incubated 1 hour, preheating D-Hanks liquid cleaning non-adherent cells, adherent 37 DEG C of incubations of macrophage
48 hours, culture supernatant is collected, cells and supernatant mixes with equivalent Griess liquid, is incubated at room temperature 10 minutes, uses NaNO2System
Make standard curve, 540nm detection NO concentration.
2.5.5TNF- α is detected
Mouse orbit takes blood, 600 × g centrifugation 15min, collects animal blood serum, contains according to kit specification detection TNF-α
Amount.
2.5.6 blood routine situation of change
7 days each group mouse all take blood through inner canthus eyeground after chemotherapy, detect blood routine.
2.5.7 hematopoietic progenitor population generative capacity detects
Put to death mouse within 7th day, sterilize after gnotobasis separating mouse femur, bone marrow cell, reference are charged and attacked with nutrient solution
Methylcellulose method, respectively with 03534 culture medium and 03334 medium culture bone marrow nucleated cell (CFU-GM) system and quick-fried formula
Colony-forming unit-erythroid (BFU-E) cultivating system, with reference to kit specification, by bone marrow cell suspension and culture medium with corresponding
Ratio is added separately to 24 orifice plates, per hole 0.5mL, is continuously cultivated in 37 DEG C, saturated humidity, 5%CO2 incubators.See within 7th day
Examine and count CFU-GM, more than 50 cells are a colony；BFU-E is counted, more than 50 cells are a colony.
2.6 statistical analysis
Experimental result all withRepresent, statistical analysis is carried out using SPSS15.0 statistical packages.Using single factor test
Variance analysis (ANOVA), compare two-by-two between group, examined using LSD methods, P ＜ 0.05 are that difference has conspicuousness.
3 results
The inhibitory action used with cyclophosphamide combined to S180 tumour growths is used alone in 3.1 Chinese medicines of the present invention
Chinese medicine group of the present invention and Chinese medicine of the present invention add CTX groups spleen index significantly to be raised compared with physiological saline group, and difference has statistics
Learn meaning (P ＜ 0.05).Compared with physiological saline group, each medication group knurl weight substantially mitigates, CTX groups knurl weight and physiological saline group phase
Than there is pole significant difference (P ＜ 0.01), Chinese medicine group of the present invention knurl weight compared with physiological saline group mitigates, statistically significant.CTX
Inhibitory rate is to 51.2%, Chinese medicine group inhibitory rate of the present invention to 38.7%.Endoxan adds Chinese medicine group inhibitory rate of the present invention
To 62.3%.It is shown in Table 3.
The inhibitory action used with cyclophosphamide combined to S180 tumour growths is used alone in 3 Chinese medicine of the present invention of table
* P ＜ 0.05, * * P ＜ 0.01, vs physiological saline groups；#P ＜ 0.05, vsCTX group##P ＜ 0.01, vsCTX group
3.2 Chinese medicines of the present invention are used alone uses the influence to spleen lymphocyte proliferation with cyclophosphamide combined
The spleen lymphocyte proliferation ability of Chinese medicine group of the present invention is higher than physiological saline group (P ＜ 0.01), no matter whether there is ConA
Intervene, Chinese medicine of the present invention adds CTX groups, and spleen lymphocyte proliferation ability significantly increases compared with CTX groups, and difference has statistics meaning
Justice.It is shown in Table 4.
4 Chinese medicine of the present invention of table is used alone to be made with cyclophosphamide combined use to S180 tumor-bearing mices lymphopoiesis
Influence
* P ＜ 0.05, * * P ＜ 0.01, vs physiological saline groups；#P ＜ 0.05, vsCTX group##P ＜ 0.01, vsCTX group
3.3 Chinese medicines of the present invention are used alone uses the influence to macrophage function with cyclophosphamide combined
Compared with physiological saline group, Chinese medicine group macrophages phagocytic capacity of the present invention, NO, TNF-α secretion are horizontal significantly to be carried
Height, difference have extremely notable meaning (P ＜ 0.01).Compared with CTX groups, Chinese medicine of the present invention adds CTX groups macrophages phagocytic capacity, NO
Secretion level raises (P ＜ 0.05).It is shown in Table 5.
5 Chinese medicine of the present invention of table is used alone uses the influence to macrophage function with cyclophosphamide combined
* P ＜ 0.05, * * P ＜ 0.01, vs physiological saline groups；#P ＜ 0.05, vs CTX group .##P ＜ 0.01, vs CTX groups
3.4 Chinese medicines of the present invention are used alone uses the influence to blood routine with cyclophosphamide combined
Compared with endoxan group, Chinese medicine group of the present invention can significantly improve WBC numbers, and it is horizontal to significantly improve HB and PLT；This hair
It is horizontal that bright Chinese medicine adds CTX groups to significantly improve HB and PLT.It is shown in Table 6.
6 Chinese medicine of the present invention of table is used alone uses the influence to blood routine with cyclophosphamide combined
* P ＜ 0.05, * * P ＜ 0.01, vs physiological saline groups；#P ＜ 0.05, vs CTX group .##P ＜ 0.01, vs CTX groups
3.5 Chinese medicines of the present invention are used alone uses the influence formed to hematopoietic progenitor population with cyclophosphamide combined
Compared with endoxan group, Chinese medicine group of the present invention, Chinese medicine of the present invention add endoxan group significantly to raise CFU-GM
And BFU-E numbers, there is obvious significant difference (P ＜ 0.05), Chinese medicine group of the present invention significantly raises BFU-E numbers, there is notable statistics
Difference (P ＜ 0.01), it is shown that Chinese medicine of the present invention is remarkably improved hematopoietic progenitor population quantity of formation after chemotherapy.It is shown in Table 7.
7 Chinese medicine of the present invention of table is used alone uses the influence formed to hematopoietic progenitor population with cyclophosphamide combined
#P ＜ 0.05, vs CTX group .##P ＜ 0.01, vs CTX groups
To sum up, Chinese medicine of the present invention has the effect of auxiliary treatment, and in terms of patients undergoing chemotherapy immunity of organisms is improved, curative effect is agreed
It is fixed, it is beneficial to the treatment of tumour, is the innovation on the medicine of adjuvant for chemotherapy of tumour, with actual clinical meaning and statistically
Meaning.
Claims (6)
1. a kind of Chinese medicine of adjuvant for chemotherapy of tumour, it is characterised in that be made up of the material medicine of following parts by weight meter：The bighead atractylodes rhizome
10-25 parts, Poria cocos 10-30 parts, the membrane of a chicken's gizzard 10-25 parts, tuber of dwarf lilyturf 5-20 part, radix polygonati officinalis 10-20 parts, scabrousstem greenbrier rhizome 5-20 parts, Selaginella tamariscina 3-
15 parts, rhizoma nelumbinis 10-20 parts, spina gleditsiae 5-20 parts, pilosella-leaved drymoglossum herb 10-30 parts and botrychiam ternatum 5-30 parts, above-mentioned raw materials medicine is mixed
Together, add water to submerge, soak 30-60min, intense fire is fried open, then is cooked by slow fire 30-40min, filtering, obtains first time filtrate, medicine
Slag adds water submergence, and intense fire is fried open, then is cooked by slow fire 30-40min, filtering, obtains second of filtrate, merges filtrate twice, mixes
.
2. the Chinese medicine of adjuvant for chemotherapy of tumour according to claim 1, it is characterised in that by the raw material of following parts by weight meter
Medicine is made：11 parts of the bighead atractylodes rhizome, 29 parts of Poria cocos, 11 parts of the membrane of a chicken's gizzard, 19 parts of the tuber of dwarf lilyturf, 11 parts of radix polygonati officinalis, 19 parts of scabrousstem greenbrier rhizome, 4 parts of Selaginella tamariscina, lotus root
6 parts of 19 parts of section, 6 parts of spina gleditsiae, 29 parts of pilosella-leaved drymoglossum herb and botrychiam ternatum, above-mentioned raw materials medicine is mixed, added water to submerge, and is soaked
30-60min, intense fire are fried open, then are cooked by slow fire 30-40min, filtering, obtain first time filtrate, the dregs of a decoction add water submergence, fried by intense fire
Open, then be cooked by slow fire 30-40min, filter, obtain second of filtrate, merge filtrate twice, mix.
3. the Chinese medicine of adjuvant for chemotherapy of tumour according to claim 1, it is characterised in that by the raw material of following parts by weight meter
Medicine is made：17 parts of the bighead atractylodes rhizome, 20 parts of Poria cocos, 18 parts of the membrane of a chicken's gizzard, 12 parts of the tuber of dwarf lilyturf, 15 parts of radix polygonati officinalis, 13 parts of scabrousstem greenbrier rhizome, 9 parts of Selaginella tamariscina, lotus root
17 parts of 15 parts of section, 13 parts of spina gleditsiae, 20 parts of pilosella-leaved drymoglossum herb and botrychiam ternatum, above-mentioned raw materials medicine is mixed, added water to submerge, and is soaked
30-60min is steeped, intense fire is fried open, then is cooked by slow fire 30-40min, filtering, obtains first time filtrate, the dregs of a decoction add water submergence, intense fire
It is fried open, then is cooked by slow fire 30-40min, filter, obtain second of filtrate, merges filtrate twice, mix.
4. the Chinese medicine of adjuvant for chemotherapy of tumour according to claim 1, it is characterised in that by the raw material of following parts by weight meter
Medicine is made：24 parts of the bighead atractylodes rhizome, 11 parts of Poria cocos, 24 parts of the membrane of a chicken's gizzard, 6 parts of the tuber of dwarf lilyturf, 19 parts of radix polygonati officinalis, 6 parts of scabrousstem greenbrier rhizome, 14 parts of Selaginella tamariscina, lotus root
29 parts of 11 parts of section, 19 parts of spina gleditsiae, 11 parts of pilosella-leaved drymoglossum herb and botrychiam ternatum, above-mentioned raw materials medicine is mixed, added water to submerge, and is soaked
30-60min is steeped, intense fire is fried open, then is cooked by slow fire 30-40min, filtering, obtains first time filtrate, the dregs of a decoction add water submergence, intense fire
It is fried open, then is cooked by slow fire 30-40min, filter, obtain second of filtrate, merges filtrate twice, mix.
5. the Chinese medicine of adjuvant for chemotherapy of tumour according to claim 1, it is characterised in that by the raw material of following parts by weight meter
Medicine is made：Bighead atractylodes rhizome 10-17 parts, Poria cocos 10-20 parts, the membrane of a chicken's gizzard 10-18 parts, tuber of dwarf lilyturf 5-12 part, radix polygonati officinalis 10-15 parts, scabrousstem greenbrier rhizome
5-13 parts, Selaginella tamariscina 3-9 parts, rhizoma nelumbinis 10-15 parts, spina gleditsiae 5-13 parts, pilosella-leaved drymoglossum herb 10-20 parts and botrychiam ternatum 5-17 parts, will be above-mentioned
Material medicine is mixed, and adds water to submerge, and soaks 30-60min, and intense fire is fried open, then is cooked by slow fire 30-40min, filtering, is obtained
First time filtrate, the dregs of a decoction add water submergence, and intense fire is fried open, then is cooked by slow fire 30-40min, filtering, obtain second of filtrate, merge
Filtrate twice, mix.
6. the Chinese medicine of adjuvant for chemotherapy of tumour according to claim 1, it is characterised in that by the raw material of following parts by weight meter
Medicine is made：Bighead atractylodes rhizome 18-25 parts, Poria cocos 21-30 parts, the membrane of a chicken's gizzard 19-25 parts, tuber of dwarf lilyturf 13-20 part, radix polygonati officinalis 16-20 parts, scabrousstem greenbrier rhizome
14-20 parts, Selaginella tamariscina 10-15 parts, rhizoma nelumbinis 16-20 parts, spina gleditsiae 14-20 parts, pilosella-leaved drymoglossum herb 21-30 parts and botrychiam ternatum 18-30 parts, will
Above-mentioned raw materials medicine mixes, and adds water to submerge, and soaks 30-60min, intense fire is fried open, then is cooked by slow fire 30-40min, mistake
Filter, obtains first time filtrate, and the dregs of a decoction add water submergence, and intense fire is fried open, then is cooked by slow fire 30-40min, filtering, obtains second of filtrate,
Merge filtrate twice, mix.
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