CN104756801B - Forced contact detection method and cage for plant leaf exterminating effect - Google Patents
Forced contact detection method and cage for plant leaf exterminating effect Download PDFInfo
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- CN104756801B CN104756801B CN201510081695.5A CN201510081695A CN104756801B CN 104756801 B CN104756801 B CN 104756801B CN 201510081695 A CN201510081695 A CN 201510081695A CN 104756801 B CN104756801 B CN 104756801B
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Abstract
The invention relates to a forced contact detection method and cage for plant leaf exterminating effect, belongs to the field of insect prevention, and aims at evaluating the exterminating effect. The method is non-damage forced contact method and applicable to the hedgerow technology, the detection of the insect exterminating effect of the medicine applied plant leaves and other related fields. The cage comprises two frameworks, and a yarn cage which sleeves the outer parts of the frameworks; the frameworks are semi-elliptical ball shaped frameworks manufactured from plastic materials with a certain of elasticity; one of the side ends of the two frameworks is connected through a hinge, and while the other one of the side ends of the two frameworks is provided with a lock buckle; the two semi-elliptical ball shaped frameworks can be closed to form an elliptical ball shaped framework; the cage yarn is prepared from smooth yarns, and openings are formed in two ends of the yarn cage; the aperture of the openings can pass through the elliptical ball shaped framework, and the length is more than that of the elliptical ball shaped framework; the opening end of the cage yarn can be tightened or released through a rope; a clamping buckle is mounted at the opening end of the yarn cage.
Description
Technical field
The present invention relates to a kind of plant leaf surface killing effect forces contact measurement method and its forces contact cage, belong to
Insect control field, evaluates killing effect, is that non-damaging forces contact method；Suitable for hedgerow technology, and dispenser plant leaf blade pair
The association areas such as insecticide killing effect measure.
Technical background
Heating in recent years takes place frequently with thrombocytopenic syndromes, Epidemic Situation of Dengue Fever, and stining for the mosquito of summer is disturbed, these by
Medium cause disease and create disturbances to not because our quality of life raising and my life of fading out completely, illustrate on the contrary
These unfavorable factors always, or all the time with around our life.In order to control infringement of these media to us, phase
The art of pass and product also emerge in an endless stream, and constantly renovate and develop.If unfavorable in these infringement controls, some diseases may
Cause bigger infringement and society panic, therefore control the needs that these factors meet the demand and the lives of the people of society.
Control Vector mode is a lot, including the process in breeding place, killing to adult mosquito and expels.Wherein chemical drugs
Agent is our commonly used means.Using method includes the spraying in space, poison bait, and residual spray etc..Residual spray is
One of conventional method, the medium in China's malaria control, prevention and control flood are grown, and the insecticide in environment, have very well
Prevention effect.With the development and progress of formulation technologies, in terms of this method also derives for others, such as mosquito proof system
Clothes, it is commonly used etc. in forest operation personnel.Worker both domestic and external developed hedgerow skill again according to its principle in recent years
Art, that is, insecticide orientation is applied to above the plant that mosquito Jing is often perched, killing action is played to adult mosquito, reduces mosquito
Density.Its advantage is purposefully to use medicine, and two is that mosquito habitat is controlled, and three can be holding effect control, and four are
The frequency of usage of medicament is reduced, dosage is reduced, environmental pollution is reduced, for this new technique does not still have a kind of to effect at present
The method for carrying out accurate evaluation.
Hedgerow technical controlling effect and efficiency time will be completed through constantly efficacy testing.Commonplace use at present
People lure thorn to sting method, this method quality relatively more directly perceived and objectively reflecting the protected effect to people, but also have deficiency
Place, is that people lures the personnel that thorn stings experiment to need to stand the pain of mosquito bite first, two be if when locality has mosquito matchmaker disease to occur,
People lures thorn to sting experimenter and can be passed disease by matchmaker and be threatened, and three is that people lures thorn to sting the lured mosquito kind of experiment to create disturbances to me with night
Mosquito kind constitute and have a larger difference, four be personnel individual variation it is very big, difference is produced to mosquito allure, result can be made
Produce deviation.In sum, people lures thorn to sting the hedgerow effect of control of experiment energy reflection part, but implements variable too much, compared with
Difficult control, and tester is constituted a threat to.The method of next is mosquito luring lamp method, and this method is convenient and simple, is easy to operation, but by outer
Boundary's interference is larger, including：Light, wind speed, the scope of hedgerow technical finesse, and Aedess mosquito etc. can not be lured, in addition, device
The susceptiveness of tool itself can also cause error, the reflection of drug effect is also had be indirectly with it is incomplete.The third method is
Plant leaf blade is taken back into laboratory test, but after leaveves once leave plant, loses activity and the vigor of life, it is impossible to
Continue degraded insecticide, blade is not easy to long-period freshness preserving and preservation after in vitro, affect the actual effect of insecticide；And little filling
Wooden blade has no idea to be tested with existing technology, if experiment frequency is very high, required blade amount is also very
Greatly, to plant and a kind of damage.
Several more universal efficacy testing methods more than comprehensive, they respectively have pluses and minuses, but key be all can not be directly anti-
Reflect whether dispenser plant living body also has killing effect.
The content of the invention
Contact measurement method is forced the present invention seeks to be directed to above-mentioned weak point and provide a kind of plant leaf surface killing effect
And its contact cage is forced, and it is a kind of according to practical work experience and conventional practice, have developed while plant is not damaged, can
Method to determine its killing effect, " plant leaf surface forces contact method to insecticide killing effect determination techniques-non-damaging " is to planting
The actual effect of thing blade is measured, and the exploitation for medicament provides simple and practical method of testing.Simultaneously to a piece of tree
Leaf can carry out repeated multiple times METHOD FOR CONTINUOUS DETERMINATION in the case of not in vitro, bring great convenience to effect measuring.It is actual to answer
Prove that this method is feasible with rear, test effect reliability.
Plant leaf surface killing effect forces contact measurement method and its forces contact cage to be to take technical scheme below to realize：
Plant leaf surface killing effect forces contact cage to include skeleton and sarong；Sarong is sleeved on outside skeleton, and skeleton is arranged
There are two, skeleton makes semiellipse spheroid skeleton using the plastic material of certain elasticity.Two one sides of skeleton are connected with hinge,
Another side can be dead with free trip buckle or be opened equipped with a lock, and two semiellipse spheroid skeletons form oval-shaped ball after closing
Skeleton.
Sarong makes sarong using silk cunning yarn, and two open ends, bore can be advisable by above-mentioned oval-shaped ball skeleton, length
It is longer than oval-shaped ball skeleton；Opening is tightened up using cotton rope or is loosened, and buckle is housed, can be with locked or opening.
Plant leaf surface killing effect forces contact measurement method as follows：
（Efficacy testing is carried out according to the following steps）
1st, the selection of blade
The blade for the treatment of region upper, middle and lower different levels is selected to carry out efficacy testing；
Selection does not compare test through the plant leaf blade of drug treating.
2nd, the selection of test worm
Worm is used as test with the standard test worm that receptacle is raised；
During without standard test worm, or when having particular/special requirement, wild test worm can be selected to use worm as test.
3rd, Preparatory work of experiment
After choosing blade, blade is sandwiched between two semiellipse spheroid skeletons with two semiellipse spheroid skeletons, will
Lock it is locked, two semiellipse spheroid skeletons close after formed oval-shaped ball skeleton, then sarong is placed on close after form ellipse
On circular spheroid skeleton, sarong rear open end is tightened up using cotton rope, and it is locked using buckle.
4th, experiment starts
Test worm to be measured is put into in sarong from sarong forward open end, test worm is no less than 50, is tightened up using cotton rope afterwards,
And the locked sarong forward open end of buckle is adopted, formation forces contact cage, and starts timing, and observing time is 0.5～1 hour.
5th, experiment terminates
At the end of experiment, the lock of two semiellipse spheroid skeletons is opened across sarong, while opening sarong after-opening
Two semiellipse spheroid skeletons are gently removed from the blade of test by the buckle at end together with sarong, in time locked sarong
Rear open end, contact cage will be forced to take back laboratory carries out recovery observation.
6th, test worm recovers observation
The recovery situation of 24 hours of above-mentioned test worm is observed using illumination box, 5% glucose of normal feeding during recovery
Water or feedstuff, record final pharmacodynamic results.
7th, experiment is completed
24 hours mortality rates of test worm are calculated and record, experiment is completed.
8th, mortality rate is calculated
9th, judge
24h mortality rates（%）>=70% is judged to effectively.
Meet《Agriculture chemical registration is with hygienic insecticide efficacy test and evaluates part 1：Propellant》（GB/
T13917.1-2009）5th and 6.2 article of pertinent regulations.
A kind of plant leaf surface killing effect forces contact measurement method and its forces contact cage, be one kind according to real work
Experience and conventional practice, have developed while plant is not damaged, the method that can determine its killing effect, " plant leaf surface
Contact method is forced to insecticide killing effect determination techniques-non-damaging " effect actual to plant leaf blade is measured, and is medicament
Exploitation is there is provided simple and practical method of testing.Simultaneously same leaf can be carried out in the case of not in vitro repeated multiple times
METHOD FOR CONTINUOUS DETERMINATION, brings great convenience to effect measuring.Prove after practical application that this method is feasible, test effect reliability.
A kind of plant leaf surface killing effect of the present invention forces contact measurement method and its forces contact cage reduce test
The labor intensity of person and threat of the mosquito to tester in test process；Reduce the inclined of the various testing results disturbed and cause
From；Directly the effect of the plant leaf blade killing mosquito of hedgerow technology is implemented in reflection.
Description of the drawings
Below with reference to accompanying drawing, the invention will be further described：
Fig. 1 is that plant leaf surface killing effect forces contact basket structure schematic diagram.
Fig. 2 is the framing structure schematic diagram that plant leaf surface killing effect forces contact cage.
Specific embodiment
Referring to the drawings 1,2, plant leaf surface killing effect forces contact cage to include skeleton 1 and sarong 2；Sarong 2 is sleeved on bone
Outside frame 1, skeleton 1 is provided with two, and skeleton 1 makes semiellipse spheroid skeleton using the plastic material of certain elasticity.Two bones
1 one side of frame is connected with hinge 3, and another side can be dead with free trip buckle or be opened, two semiellipse spheroid bones equipped with a lock 4
Frame forms oval-shaped ball skeleton after closing.
Sarong 1 makes sarong, two open ends using silk cunning yarn, and bore by above-mentioned oval-shaped ball skeleton can be just
Preferably, length is more slightly longer than oval-shaped ball skeleton；Opening is tightened up or is loosened using cotton rope 6, and buckle 5 is housed, can with locked or
Open.
Plant leaf surface killing effect forces contact measurement method as follows：
（Efficacy testing is carried out according to the following steps）
1st, the selection of blade
The blade for the treatment of region upper, middle and lower different levels is selected to carry out efficacy testing；
Selection does not compare test through the plant leaf blade of drug treating.
2nd, the selection of test worm
Worm is used as test with the standard test worm that receptacle is raised；
During without standard test worm, or when having particular/special requirement, wild test worm can be selected to use worm as test.
3rd, Preparatory work of experiment
After choosing blade, blade is sandwiched between two semiellipse spheroid skeletons with two semiellipse spheroid skeletons 1, will
Latch 4 locked, two semiellipse spheroid skeletons 1 form oval-shaped ball skeleton after closing, then sarong 2 is placed on close after formed
On oval-shaped ball skeleton, 2 rear open end of sarong is tightened up using cotton rope 6, and it is locked using buckle 5.
4th, experiment starts
Test worm to be measured is put into in sarong 2 from 2 forward open end of sarong, test worm is no less than 50, is received using cotton rope 6 afterwards
Tightly, and using 5 locked sarong of buckle, 2 forward open end, formation forces contact cage, and starts timing, and observing time is 0.5～1 little
When.
5th, experiment terminates
At the end of experiment, the lock 4 of two semiellipse spheroid skeletons is opened across sarong 2, while opening after opening sarong 2
Two semiellipse spheroid skeletons are gently removed from the blade of test by the buckle 5 at mouth end together with sarong 2, locked in time
2 rear open end of sarong, contact cage will be forced to take back laboratory carries out recovery observation.
6th, test worm recovers observation
The recovery situation of 24 hours of above-mentioned test worm is observed using illumination box, 5% glucose of normal feeding during recovery
Water or feedstuff, record final pharmacodynamic results.
7th, experiment is completed
24 hours mortality rates of test worm are calculated and record, experiment is completed.
8th, mortality rate is calculated
9th, judge
24h mortality rates（%）>=70% is judged to effectively.
Meet《Agriculture chemical registration is with hygienic insecticide efficacy test and evaluates part 1：Propellant》（GB/
T13917.1-2009）5th and 6.2 article of pertinent regulations.
Claims (4)
1. a kind of plant leaf surface killing effect forces contact cage, it is characterised in that：Including skeleton and sarong；Sarong is sleeved on skeleton
Outside, skeleton are provided with two, and skeleton makes semiellipse spheroid skeleton, two skeleton sides using the plastic material of certain elasticity
End is connected with hinge, and equipped with a lock, two semiellipse spheroid skeletons form oval-shaped ball skeleton after closing to another side.
2. a kind of plant leaf surface killing effect according to claim 1 forces contact cage, it is characterised in that：Sarong adopts silk
Sliding yarn makes sarong, and two open ends, bore can be longer than oval-shaped ball skeleton by oval-shaped ball skeleton, length.
3. a kind of plant leaf surface killing effect according to claim 1 and 2 forces contact cage, it is characterised in that：Sarong is opened
Mouth end is tightened up using cotton rope or is loosened, and buckle is housed.
4. a kind of plant leaf surface killing effect forces contact measurement method, it is characterised in that：
（1）The selection of blade
The blade for the treatment of region upper, middle and lower different levels is selected to carry out efficacy testing；
Selection does not compare test through the plant leaf blade of drug treating；
（2）The selection of test worm
Worm is used as test with the standard test worm that receptacle is raised；
During without standard test worm, or when having particular/special requirement, wild test worm can be selected to use worm as test；
（3）Preparatory work of experiment
After choosing blade, blade is sandwiched between two semiellipse spheroid skeletons, with lock with two semiellipse spheroid skeletons
Locked, two semiellipse spheroid skeletons form oval-shaped ball skeleton after closing, then sarong is placed on close after the ellipse that formed
On shape spheroid skeleton, sarong rear open end is tightened up using cotton rope, and it is locked using buckle：
（4）Experiment starts
Test worm to be measured is put in sarong from sarong forward open end, test worm is no less than 50, is tightened up using cotton rope afterwards, and is adopted
The locked sarong forward open end of buckle, formation force contact cage, and start timing, and observing time is 0.5～1 hour；
（5）Experiment terminates
At the end of experiment, the lock of two semiellipse spheroid skeletons is opened across sarong, while opening sarong rear open end
Two semiellipse spheroid skeletons are gently removed from the blade of test by buckle together with sarong, are opened in time after locked sarong
Mouth end, contact cage will be forced to take back laboratory carries out recovery observation；
（6）Test worm recovers observation
Observe the recovery situation of 24 hours of above-mentioned test worm using illumination box, during recovery 5% G/W of normal feeding or
Feedstuff, records final pharmacodynamic results；
（7）Experiment is completed
24 hours mortality rates of test worm are calculated and record, experiment is completed；
（8）Mortality rate is calculated
（9）Judge
24h mortality rates（%）>=70% is judged to effectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510081695.5A CN104756801B (en) | 2015-02-15 | 2015-02-15 | Forced contact detection method and cage for plant leaf exterminating effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510081695.5A CN104756801B (en) | 2015-02-15 | 2015-02-15 | Forced contact detection method and cage for plant leaf exterminating effect |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104756801A CN104756801A (en) | 2015-07-08 |
| CN104756801B true CN104756801B (en) | 2017-03-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510081695.5A Active CN104756801B (en) | 2015-02-15 | 2015-02-15 | Forced contact detection method and cage for plant leaf exterminating effect |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720194A (en) * | 2007-06-29 | 2010-06-02 | 韦斯特高凡德森有限公司 | Insecticidal barrier with a durable lower part |
| CN103215751A (en) * | 2012-01-19 | 2013-07-24 | 佛山市南海必得福无纺布有限公司 | Long-acting insect prevention and mosquito repelling PP (Polypropylene) non-woven fabric and preparation method thereof |
| CN203630116U (en) * | 2013-12-24 | 2014-06-04 | 胡津镐 | Forced contactor |
| CN204498950U (en) * | 2015-02-15 | 2015-07-29 | 江苏省疾病预防控制中心 | Plant leaf surface killing effect forces contact cage |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4252100B1 (en) * | 2007-09-26 | 2009-04-08 | 昭 問山 | Solid molding |
| JP2009263251A (en) * | 2008-04-22 | 2009-11-12 | Saburo Uemori | Mosquito-repelling water, method for producing the same and mosquito-repelling method |
-
2015
- 2015-02-15 CN CN201510081695.5A patent/CN104756801B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720194A (en) * | 2007-06-29 | 2010-06-02 | 韦斯特高凡德森有限公司 | Insecticidal barrier with a durable lower part |
| CN103215751A (en) * | 2012-01-19 | 2013-07-24 | 佛山市南海必得福无纺布有限公司 | Long-acting insect prevention and mosquito repelling PP (Polypropylene) non-woven fabric and preparation method thereof |
| CN203630116U (en) * | 2013-12-24 | 2014-06-04 | 胡津镐 | Forced contactor |
| CN204498950U (en) * | 2015-02-15 | 2015-07-29 | 江苏省疾病预防控制中心 | Plant leaf surface killing effect forces contact cage |
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| Publication number | Publication date |
|---|---|
| CN104756801A (en) | 2015-07-08 |
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| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Dapeng Inventor after: Liu Hui Inventor after: Chu Hongliang Inventor before: Liu Dapeng Inventor before: Liu Hui Inventor before: Chu Hongliang |
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| COR | Change of bibliographic data | ||
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| GR01 | Patent grant |