CN104749086A - Multi-reference-channel pulse recognition method and device, and particle analyzer - Google Patents
Multi-reference-channel pulse recognition method and device, and particle analyzer Download PDFInfo
- Publication number
- CN104749086A CN104749086A CN201310754674.6A CN201310754674A CN104749086A CN 104749086 A CN104749086 A CN 104749086A CN 201310754674 A CN201310754674 A CN 201310754674A CN 104749086 A CN104749086 A CN 104749086A
- Authority
- CN
- China
- Prior art keywords
- pulse
- reference channel
- data district
- time zone
- invalid data
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The application discloses a multi-reference-channel pulse recognition method and device, and a particle analyzer. The multi-reference-channel pulse recognition method comprises the following steps of: for single reference channels discovering pulses, on the basis of the discovered pulses to be recognized, determining invalid data areas in the data to be processed of the single reference channels; unifying starting time of the invalid data areas of the plurality of reference channels according to the invalid data areas of all the single reference channels; on the basis of the invalid data areas with unification of the starting time, determining valid data areas of the plurality of reference channels; and when the pulse recognition is carried out, carrying out pulse recognition on the pulses of the valid data areas instead of the pulses of the invalid data areas. The multi-reference-channel pulse recognition method, the device and the particle analyzer disclosed by the application have the advantages that data which can not or is difficult for obtaining correct area, amplitude and width information is eliminated by a mode of determining the invalid data areas with unified starting time among the plurality of reference channels, and the pulse recognition is not carried out, so that the data processing amount in pulse recognition is reduced.
Description
Technical field
The application relates to a kind of Medical Instruments, particularly relates to the many reference channels pulse recognition method and apparatus in a kind of cytoanalyze.
Background technology
Stream type cell analyzer is that one (is referred to as particle for analysis of cells or molecule, be described for cell below) the instrument of physicochemical characteristic, its principle as shown in Figure 1, flow cytometer generally adopts sheath Flow Technique, the cell in sample is made to be flow through flow chamber 1 by the sequence of sheath fluid parcel, exciting light 2 is radiated on cell 3 one by one, cell 3 is made to produce scattered light or fluorescence signal, after being collected by light signal, after photoelectric commutator 5 is changed, treated circuit 6 forms electric impulse signal 4 after processing again, its amplitude (H) is obtained after pulse recognition being carried out to this pulse signal by pulse recognition module 7, area (A), after the parameters such as width (W), show finally by patterned mode, analyze for user.The parameters such as H, A, W of these scattered lights or fluorescence signal, can reflect the content etc. of inhereditary material in the expression of the size of cell, granularity and antigen molecule, cell, and then from clinical medicine or biology angle, obtain valuable reference information.Therefore the parameter such as amplitude, area, width correctly identifying the various cell formation pulse signal that is excited is significant.
Various kinds of cell may be had in the sample measured, after each cell is excited, generally all can produce forward scattering light (FSC) and side scattered light (SSC), but whether fluorescence channel has signal, then according to the fluorescein on cell characteristic and cell with or without being divided into following several situation: do not have, only have a road, have multichannel fluorescence signal.Analyze this cell and produce the information such as H, A, W of signal at different passage, some characteristic informations about this cell can be drawn.Due to may various kinds of cell be contained in sample, and user is only interested in some specific cells, at this moment threshold value is set often through at certain passage (being called " reference channel "), the pulse making peak value in this passage be less than threshold value be left in the basket, the be greater than pulse of threshold value is collected and then analyzes information such as drawing H, A, W, analyzes and show that the process of the information such as H, A, W of pulse is called pulse recognition.
In some cases, the signal of user to some passage is interested, then these passages are set to reference channel, in multiple " reference channel ", threshold value is set, then combinational logic ("AND" "or") computing is carried out to the threshold decision result of these passages, judge whether analyze this cell and provide the information such as H, A, W according to the result drawn.
Summary of the invention
The application provides the method and apparatus of the many reference channels pulse recognition in a kind of cytoanalyze, reduces the data operation quantity in many reference channels pulse recognition process.
According to the first aspect of the application, the application provides a kind of many reference channels pulse recognition method, comprising:
The signal gathering the output of multiple reference channel obtains the pending data of each reference channel, and the signal that described reference channel exports is the electric signal that the optical information sent after the tested particle collected by reference channel is optically excited converts to;
Based on the pending data of each single reference channel with whether find pulse with this reference channel characteristic of correspondence threshold decision, described characteristic threshold value is the threshold value of the feature of interest of particle of interest, and described discovery pulse refers to and detects that peak value of pulse is greater than the pulse of characteristic threshold value;
For the single reference channel finding pulse, determine the invalid data district in the pending data of this single reference channel based on the pulse to be identified found;
According to the beginning and ending time in the invalid data district of the unified multiple reference channel in the invalid data district of each single reference channel;
The valid data district of multiple reference channel is determined based on the invalid data district after the unified beginning and ending time;
When carrying out pulse recognition, pulse recognition is carried out to the pulse in valid data district, no longer pulse recognition is carried out to the pulse in invalid data district.
According to the second aspect of the application, the application provides a kind of many reference channels pulse recognition device, comprising:
Data acquisition unit, the signal exported for gathering multiple reference channel obtains the pending data of each reference channel, and the signal that described reference channel exports is the electric signal that the optical information sent after the tested particle collected by reference channel is optically excited converts to;
Pulse finds unit, for the pending data based on each single reference channel with whether find pulse with this reference channel characteristic of correspondence threshold decision, described characteristic threshold value is the threshold value of the feature of interest of particle of interest, and described discovery pulse refers to and detects that peak value of pulse is greater than the pulse of characteristic threshold value;
Single channel dead space determining unit, for for the single reference channel finding pulse, determines the invalid data district in the pending data of this single reference channel based on the pulse to be identified found;
Dead space Unified Element, for the beginning and ending time in the invalid data district according to the unified multiple reference channel in the invalid data district of each single reference channel;
Effective district determining unit, determines the valid data district of multiple reference channel based on the invalid data district after the unified beginning and ending time;
Pulse recognition unit, for carrying out pulse recognition when carrying out pulse recognition to the pulse in valid data district, no longer carries out pulse recognition to the pulse in invalid data district.
According to the third aspect of the application, the application provides a kind of particle analyzer, comprising:
Excitation source, for providing the exciting light irradiating tested particle;
Flow chamber, for providing tested particle irradiated place;
Light collecting device, for collecting the optical information sent after tested particle is optically excited, described light collecting device comprises multiple reference channel; With
Above-mentioned many reference channels pulse recognition device.
The application by determining the mode in the invalid data district that the beginning and ending time is unified between multiple reference channel, or cannot be difficult to draw that the data of correct area, amplitude and width information are got rid of, pulse recognition is not carried out to it, thus data processing amount when decreasing pulse recognition.
Accompanying drawing explanation
Fig. 1 is flow cytometer fundamental diagram;
Fig. 2 is the structural representation of flow cytometer;
Fig. 3 is the structural representation of the hyperchannel pulse recognition device of a kind of embodiment of the application;
Fig. 4 is pulse shape signature analysis figure;
Fig. 5 is the process flow diagram of many reference channels pulse recognition of a kind of embodiment of the application;
Fig. 6 is the process flow diagram for single reference channel determination invalid data district in a kind of embodiment;
Fig. 7 is the process flow diagram of the beginning and ending time in the invalid data district of unified multiple reference channel in a kind of embodiment;
Fig. 8 is one of schematic diagram longitudinally polluting and laterally pollute;
Fig. 9 is the schematic diagram two longitudinally polluting and laterally pollute;
Figure 10 is a kind of structural representation of effective district determining unit of a kind of embodiment of the application;
Figure 11 is the another kind of structural representation of effective district determining unit of a kind of embodiment of the application;
Figure 12 is the process flow diagram that effective district of a kind of embodiment of the application determines;
Figure 13 is the schematic diagram determining invalid data district according to similarity rule;
Figure 14 is the overlapping schematic diagram of pulse window in a kind of embodiment of the application.
Embodiment
By reference to the accompanying drawings the present invention is described in further detail below by embodiment.
Flow cytometer is a kind of particle analyzer, the embodiment of the present application adopts flow cytometer to carry out determination and analysis to particle, the structure of flow cytometer as shown in Figure 2, comprise excitation source 1025, flow chamber 1022, light collecting device and processor 1026, excitation source 1025 irradiates the exciting light of tested particle for providing, such as laser; Flow chamber 1022 is for providing tested particle irradiated place 1021; Light collecting device is for collecting the optical information sent after tested particle is optically excited, and it comprises all reference channels and non-reference passage; Each passage includes optical module 1023 and electrooptical device 1024, optical module 1023 is for completing the collection, shaping etc. of scattered light or fluorescence, electrooptical device 1024 is for being converted to electric signal by the optical information of collection and outputting to processor 1026, and processor 1026 is for processing electric signal.
In order to analytic process is described, the term used in the application is made an explanation.
Reference channel and non-reference passage: as shown in Figure 1, a particle can produce pulse signal at multiple passage.For certain particle of interest, if user is in n(n >=2) individual passage is provided with the characteristic threshold value relevant to the feature of interest of this particle of interest, then all be called " reference channel " by this n passage, remaining passage is called as " non-reference passage ".As user is provided with the characteristic threshold value relevant to the feature of interest of this particle of interest at each passage, then passage is all reference channel, and does not have non-reference passage.
Find pulse: user is a reference point relevant to the feature of interest of particle of interest in the characteristic threshold value that reference channel is arranged, if found the data being greater than this reference point in this passage, has namely thought and discovery pulse triggers identifying thus.If there is no the data finding to be greater than this reference point, then think and do not find pulse.May have in reference channel a lot " small-pulse effect ", but be not more than the threshold value of user's setting, so can not be found due to it.
Invalid data district: the object of pulse recognition is the information such as A, H, the W of the pulse that will calculate the existence of each passage.If the data in some region cannot or be difficult to draw correct A, H, W information, so conveniently this part data being set to invalid data to process, discriminance analysis not being carried out to it.This segment data district is exactly " invalid data district "; The object done like this makes the Project Realization of algorithm more simple and feasible.
Valid data district: district is corresponding with invalid data, need the data participating in pulse recognition to be valid data, this segment data district is exactly " valid data district ".
Laterally pollute and longitudinally pollute: because certain section " invalid data district " causes another part data also to become in " invalid data district " according to certain rule, then claiming the latter by " pollution "; Laterally pollute and refer to the pollution carried out in same reference channel, longitudinally pollute and refer to the pollution carried out between multiple reference channel.
Pulse combined: same particle has certain similarity in the pulse that multiple interchannel is formed, such as similar width, peak value moment are close, one_to_one corresponding etc., utilize this similarity to be that the process of the pulse that same effective particle sends is called pulse combined by the pulse one_to_one corresponding of different reference channel.
In the embodiment of the present application, first in reference channel, determine the invalid data district with the unified beginning and ending time, and then determine valid data district, only pulse recognition process is carried out to the data in valid data district when carrying out pulse recognition, thus the data processing amount decreased in pulse recognition process, and because having abandoned the data that some can not be obtained a result, thus also improve the accuracy of all kinds of result after pulse recognition.
Embodiment one:
Processor 1026 in Fig. 2 comprises hyperchannel pulse recognition device, for carrying out pulse recognition to the multichannel electrical signal of input processor 1026, as shown in Figure 3, hyperchannel pulse recognition device 30 comprises data acquisition unit 31, pulse discovery unit 32, single channel dead space determining unit 33, dead space Unified Element 34, effectively district's determining unit 35 and pulse recognition unit 36.
Data acquisition unit 31 obtains the pending data of each reference channel for the signal gathering the output of multiple reference channel, and the signal that reference channel exports is the electric signal that the optical information sent after the tested particle collected by reference channel is optically excited converts to.
Pulse discovery unit 32 is for the pending data based on each single reference channel and whether find pulse with this reference channel characteristic of correspondence threshold decision, characteristic threshold value is the threshold value of the feature of interest of particle of interest, finds that pulse refers to and detects that peak value of pulse is greater than the pulse of characteristic threshold value.As shown in Figure 4, the output waveform after processing of circuit maintains the baseline voltage 41 of a direct current when not having particle luminous, have particle to pass through and create scattered light signal, then produce a pulse signal.When this pulse amplitude (part more than baseline) exceedes set characteristic threshold value 42, then think and find a pulse.Pulse area between former and later two intersection points (i.e. threshold point) of pulse and characteristic threshold value is called threshold window 43, before and after threshold point respectively to front, backward expand one section of region, form front expanded window 44 and rear expanded window 45, front and back expanded window 44 45 jointly form pulse window 46 with threshold window 43.The spreading range of front and back expanded window, the characteristic sum user use habit according to tested object is determined, in general will ensure that the scope after expanding can cover whole pulse waveform scope.
Single channel dead space determining unit 33, for for the single reference channel finding pulse, determines the invalid data district in the pending data of this single reference channel based on the pulse to be identified found.If find in identifying that the pulse window of this pulse has overlapping with the pulse window of front and back pulse, then think that these pulses are idler Pulse, this segment data abandons and does not re-use.In addition when finding that the width of this pulse is too wide or too narrow, also thinking that this is a spurious pulse, being considered as idler Pulse.The time period that idler Pulse window relates to is confirmed as invalid data district.Therefore, in a kind of instantiation, single channel dead space determining unit 33 comprises pulse window determination subelement, the first judgment sub-unit and the second judgment sub-unit, and pulse window determination subelement is for determining the pulse window of pulse to be identified, and pulse window is the region of pulse continuity; First judgment sub-unit, for judging that whether the pulse window of current pulse to be identified is overlapping with the pulse window of pulse to be identified adjacent before and after it, if so, then will relate to overlapping pulse window region as invalid data district; Whether the second judgment sub-unit abnormal for judging the pulse width of pulse to be identified, if so, then using the pulse window region of the pulse to be identified of this exception as invalid data district.
Dead space Unified Element 34 is for the beginning and ending time in the invalid data district according to the unified multiple reference channel in the invalid data district of each single reference channel.In an instantiation, dead space Unified Element comprises longitudinal direction and pollutes subelement, laterally pollutes subelement and the 3rd judgment sub-unit.Longitudinally pollute subelement to be used for one by one single reference channel with invalid data district being compared as by than reference channel with other reference channel, using in the pending data area of other reference channel with by identical time period beginning and ending time in the invalid data district than reference channel as invalid data district; Laterally pollute subelement for judging whether the invalid data district of each single reference channel pulse window adjacent with front and back has overlapping, if had, then relates to the pulse window region of overlap also as invalid data district; 3rd judgment sub-unit, for judging whether the invalid data district of each single reference channel has the unified beginning and ending time, is determined to work on or end loop by longitudinally polluting subelement and laterally polluting subelement according to judged result.
Effective district determining unit 35 determines the valid data district of multiple reference channel based on the invalid data district after the unified beginning and ending time, such as, be valid data district by the data field in whole data field except invalid data district.
Pulse recognition unit 36, for carrying out pulse recognition when carrying out pulse recognition to the pulse in valid data district, no longer carries out pulse recognition to the pulse in invalid data district.
Based on many reference channels pulse recognition device of the present embodiment, when identifying multichannel pulse, its treatment scheme as shown in Figure 5, comprises following flow process:
Step 50, gathers the signal that multiple passage exports, obtains the pending data of each passage, and the signal that each passage exports is the electric signal that the optical information sent after the tested particle collected by each passage is optically excited converts to.Multiple passage, scattered light signal or fluorescence signal are collected through each passage, simulating signal continuous in time is defined carry out opto-electronic conversion, signal processing circuit process in each passage after, utilize the AD device with certain sampling rate to carry out sample conversion to this signal, simulating signal is become discrete digital signal.Each sampling obtains the digital quantity of analog voltage signal at that time, and so each sampled point is in this article referred to as " point ".Because the sampling of AD device transforms, continuous print simulating signal is become " point " of a series of digital quantity, because " point " is very intensive, can the waveform of restoration simulation signal substantially after connecting.The follow-up said Wave data of the application, all refers to the digital data after AD turns.Mode subsequently through software or logical circuit carries out analyzing and processing to Wave data.
Step 51, based on the pending data of each single reference channel with whether find pulse with this reference channel characteristic of correspondence threshold decision, described characteristic threshold value is the threshold value of the feature of interest of particle of interest.In the present embodiment, in multiple passage, at least arrange two reference channels, the Wave data first for reference channel processes, and judges whether be found pulse in each single reference channel.As shown in Figure 4, think discovery pulse when detecting that the peak value of certain pulse is greater than characteristic threshold value, this pulse is called pulse to be identified.If the peak value of pulse is less than or equal to characteristic threshold value, then abandon this pulse.
Step 52, for the single reference channel finding pulse, determines the invalid data district in the pending data of this single reference channel based on the pulse to be identified found; If do not find pulse in certain reference channel, then whether this single reference channel uncertain has invalid data field.When determining the invalid data district of single reference channel, if detect that pulse window has overlap, or pulse width has exception, all belongs to idler Pulse, and its time period related to is invalid data district.Concrete treatment scheme as shown in Figure 6, comprises the following steps:
Step 60, the threshold window of feature based threshold test pulse to be identified, described threshold window is the pulse area between pulse to be identified two threshold point crossing with the boundary that characteristic threshold value is formed.
Step 61, obtains pulse window by threshold window respectively to after front and rear expansion presumptive area.Pulse window is the region of pulse continuity, in order to the region making pulse window cover pulse continuity completely, to prevent missing data, pulse window is obtained after threshold window is expanded one section of predetermined region respectively to front and rear, as shown in Figure 4, obtain front expanded window 44 after threshold window 43 expanded forward presumptive area, obtain rear expanded window 45 after expanding presumptive area backward, the time period of pulse window 46 equals front expanded window 44 and adds that threshold window 43 adds rear expanded window 45.Expansion have following effect: 1, expansion after can in expanded window integral and calculating pulse area; 2, by expansion, can the difference of pulse on generation time between redundant channel, ensure in expanded window, when calculating the area of each passage, whole pulse can be lived by ensuring coverage.Before and after expanded window width setting and characteristic threshold value be provided with certain relation, if characteristic threshold value arrange more close to pulse apex, then need to expand and could cover whole pulse greatlyr, threshold value close to bottom, is then expanded and is not needed just can cover very greatly.
Step 62, judges that whether the pulse window of current pulse to be identified is overlapping with the pulse window of pulse to be identified adjacent before and after it, if so, then performs step 63, otherwise performs step 64.
Step 63, will relate to overlapping pulse window region as invalid data district.When pulse window has overlap, area or the areal calculation that effectively cannot calculate this pulse are inaccurate, if therefore pulse window has overlap, then the time period front and back pulse window relating to overlap contained, as invalid data district, does not participate in follow-up pulse recognition.
Step 64, judges that whether the pulse width of pulse to be identified is abnormal, if so, then performs step 63, using the pulse window region of the pulse to be identified of this exception as invalid data district.Otherwise perform step 65, continue to detect next pulse to be identified.
Under normal circumstances, after tested particle is excited produce the pulse corresponding to light have certain characteristic, even if detect by different passages, the pulse shape of each passage also has consistance, and generation time is identical.Therefore, the pulse shape that can produce according to this kind of tested particle judges whether pulse is that effective particle produces.In the present embodiment, pulse width is adopted to reflect pulse shape, such as can using half-peak breadth (i.e. the pulse width at peak value half place) or equivalent width as pulse width, when pulse width is excessive or many hours, can think that pulse shape does not meet, then this pulse is likely undesired signal, and therefore the pulse of pulse width exception is considered to idler Pulse, time period involved by its pulse window is considered to invalid data district, does not participate in follow-up pulse recognition.
Step 53, according to the beginning and ending time in the invalid data district of the unified multiple reference channel in the invalid data district of each single reference channel.By the beginning and ending time in the invalid data district of unified each reference channel, maybe cannot show that the data of legitimate result are got rid of by less desirable, thus data processing amount when decreasing pulse recognition.In a kind of specific embodiment, by the beginning and ending time in the invalid data district of the unified multiple reference channel of following steps, as shown in Figure 7.
Step 70, longitudinally pollutes step.One by one single reference channel with invalid data district is compared as by than reference channel with other reference channel, using in the pending data area of other reference channel with by identical time period beginning and ending time in the invalid data district than reference channel as invalid data district.After longitudinally polluting, the invalid data district of multiple reference channel has identical start time and end time.
Step 71, laterally pollutes step.Judge whether the pulse window that after longitudinally polluting, the invalid data district of each single reference channel is adjacent with front and back has overlapping, if had, then relate to overlapping pulse window region also as invalid data district.In each reference channel, because data buffer area is limited, along with the carrying out identified will remove legacy data, increase new data.Therefore in a preferred embodiment, only retain the low volume data before the pulse recognition moment, the size of data volume can ensure that the previous pulse data overlapping with current PRF can find in buffer zone, but can not ensure that more data also can find before this.So have overlapping previous pulse window only to pollute once with invalid data district, later has the sustainable pollution of overlapping pulses window.That is: in time having one piece of data district to become invalid data district, if then the time have overlap early than its pulse window of a pulse produced with this section of invalid data or contact, then contaminated one-tenth invalid data district, this pulse window region, the two is connected as a single entity, entirely become invalid data district, if this segment data front also has pulse window, then this pulse window is no longer polluted, and its recognition result retains.If there is overlap in one section of invalid data district with the pulse window produced afterwards or contacts, the pulse window produced is then contaminated, the two is connected as a single entity, if back has again pulse window to contact with it, then three is connected as a single entity, entirely become invalid data district, can go down by chronic pollution, this is called chronic pollution always.The beginning and ending time performing the invalid data district of each reference channel after laterally polluting may be different, therefore performs step 72.
Step 72, judges that whether the beginning and ending time in the invalid data district of multiple reference channel is consistent, if the beginning and ending time in the invalid data district of each reference channel is identical, then and end loop, otherwise turn to execution step 70.
Circulation like this, until detect after and " the invalid data district " of all reference channels all has identical start time and end time.Longitudinally pollute and laterally pollute as shown in Figure 8, pulse window T3-T4 shown in pulse window T1-T2 shown in solid line in reference channel 2 and dotted line there occurs pulse window overlap, cause the data invalid in the pulse window region (the T1-T4 region namely in figure) of all coherent pulses in passage 2, according to longitudinal pollution, in the same time section of passage 1, data field (T1-T4) is also invalid, and then according to horizontal pollution, the pulse window region T5-T6 of passage 1 also becomes invalid data, and then according to longitudinal pollution, (i.e. passage 1 shown in figure and passage 2) region T1-T6 of all reference channels all becomes invalid data district.Again as shown in Figure 9, the pulse width of passage 1 is abnormal, pulse is wide, and pulse window T3-T4 becomes invalid data district, according to longitudinal pollution, the T3-T4 region of passage 2 also becomes invalid data district, T3-T4 region again due to passage 2 is overlapping with pulse window T1-T2, according to horizontal pollution, causes pulse window T1-T2 to become invalid data district, again according to longitudinal pollution, the T1-T4 region of all reference channels is finally caused all to become invalid data district.
Step 54, determines the valid data district of multiple reference channel based on the invalid data district after the unified beginning and ending time.Valid data district can be the data field after invalid data district is got rid of in whole data field, also can determine effective data field by other means.Valid data district should be not overlapping with invalid data district.
According to each reference channel, whether step 55, find that the logical value of each reference channel is determined in pulse.In each reference channel, according to the rule of " find pulse be then 1 ", " not finding that pulse is then 0 ", be " 1 " or " 0 " at each reference channel by its pulse validity Chx_Valid assignment.
Step 56, carries out logical operation based on the logical value of each reference channel and logical algorithm.UI operation interface can be selected multiple passage do "AND" logical operation and still do "or" logical operation, based on different situations, user may have different selections, according to the selection of user, the Chx_Valid of each reference channel is carried out logical operation and obtain the final validity result ChAll_Valid of many reference channels.
Step 57, determines whether to carry out pulse recognition to the pending data of all passages according to logic operation result, if so, then performs step 58, otherwise performs step 59.Such as, if net result ChAll_Valid is " 0 ", then the valid data district of all passages is also set in " invalid data district ", no longer carries out pulse recognition.If net result ChAll_Valid is " 1 ", then continue to perform step 58.
Step 58, carries out pulse recognition to the pending data of all passages, only carrying out pulse recognition to the pulse in valid data district, no longer carrying out pulse recognition to the pulse in invalid data district when carrying out pulse recognition.Calculate A, H, W information of the pulse in valid data district during pulse recognition and export.
Step 59, terminates.
After the present embodiment is polluted by vertical and horizontal, all width is abnormal, pulse window the is overlapping pulse window region corresponding to pulse is all removed, the region being about to can not carry out effectively calculating is decided to be invalid data district, when carrying out pulse recognition, pulse recognition computing is not carried out to invalid data district, thus decrease data operation quantity.Due to the normally pulse that effectively calculates of disturbing pulse or cannot carry out of the pulse in invalid data district, after therefore removing these pulses, also improve the accuracy of result of calculation simultaneously.
Embodiment two:
According to above-described embodiment, after determining invalid data district, determine effective data field further, the data only for valid data district during follow-up identification pulse carry out pulse recognition computing.The difference of the present embodiment and above-described embodiment there are provided one and preferably determines scheme in valid data district.
In the present embodiment, effective district determining unit is used for the similarity rule between the pulse that formed at multiple reference channel according to same particle, by carrying out pulse combined to the data area except invalid data district of multiple reference channel, to find that single reference channel of pulse is as by than reference channel one by one when pulse combined, using by than the pulse window in the data area except invalid data district of reference channel as by than time zone.Determine the valid data district of multiple reference channel according to pulse combined result, valid data district at least comprises the successful pulse window region of pulse combined.
In a kind of instantiation, as shown in Figure 10, similarity rule comprises: the pulse in the respective pulses window of multiple reference channel is no more than 1.Effective district determining unit 351 comprises threshold point and obtains subelement 3510, the 4th judgment sub-unit 3511, the 5th judgment sub-unit 3512 and the 6th judgment sub-unit 3513.Threshold point obtain subelement 3510 for obtain other each reference channel by than the threshold point in time zone; Whether the 4th judgment sub-unit 3511 single is equaled 0 by the quantity than the threshold point in time zone (namely single by than time zone) for what judge other reference channel, if the quantity of threshold point equals 0, this that this reference channel is then described is by than no pulse in time zone, the quantity of threshold point is not equal to 0, then the quantity of threshold point may be 1,2,3 even more.Directly think that when the quantity of threshold point equals 0 this is by more successful than the pulse combined in time zone; 5th judgment sub-unit 3512, for when the quantity of the 4th judgment sub-unit 3511 judgment threshold point is not equal to 0, judges whether list is equaled 2 by the quantity than the threshold point in time zone.If equal 2, then this that this reference channel is described is by than only having a pulse in time zone.If be not equal to 2, then this that this reference channel is described by than there being more than one pulse in time zone, such as 1.5 or 2, then thinks that this is by than the pulse combined failure in time zone in this case, 6th judgment sub-unit 3513 for judge in the 5th judgment sub-unit 3512 single equaled 2 by the quantity than the threshold point in time zone time judge that the time of front threshold point in these two threshold point is whether prior to the time of rear threshold point, if, this that this reference channel is then described is by than only having a pulse in time zone, think that this is by more successful than the pulse combined in time zone, otherwise think that this is by more failed than the pulse combined in time zone, this of this reference channel is by than in time zone being the aft section of previous pulse and the previous section of a rear pulse.
In another kind of instantiation, as shown in figure 11, similarity rule comprises: the pulse in the respective pulses window of multiple reference channel is no more than 1 and peak value moment is close.Effective district determining unit 352 comprises threshold point and obtains subelement 3520, the 4th judgment sub-unit 3521, the 5th judgment sub-unit 3522, the 6th judgment sub-unit 3523 and the 7th judgment sub-unit 3524.Threshold point obtain subelement 3520 for obtain other each reference channel by than the threshold point in time zone; 4th judgment sub-unit 3521, for judging whether the single of other reference channel is equaled 0 by the quantity than the threshold point in time zone, if so, then thinks that this is by more successful than the pulse combined in time zone; 5th judgment sub-unit 3522, for judging whether list is equaled 2 by the quantity than the threshold point in time zone, if be not equal to 2, then thinks that this is by more failed than the pulse combined in time zone; 6th judgment sub-unit 3523 for judge in the 5th judgment sub-unit single equaled 2 by the quantity than the threshold point in time zone time judge that the time of front threshold point in these two threshold point is whether prior to the time of rear threshold point, if the time of front threshold point is later than the time of rear threshold point, then think that this is by more failed than the pulse combined in time zone; When 7th judgment sub-unit 3524 is for the time of time of threshold point before the 6th judgment sub-unit judges prior to rear threshold point, judge whether the same of all reference channels is greater than interval threshold by than the difference between the peak value of pulse moment in time zone, if, then think that this is by more failed than the pulse combined in time zone, otherwise think that this is by more successful than the pulse combined in time zone.
Because same particle has certain similarity in the pulse that multiple interchannel is formed, such as similar width, peak value moment is close, (particle can not form more than 2 pulses at a passage to one_to_one corresponding, can only be 0 or 1 pulse) etc., if the pulse between discovery reference channel does not meet these features, then think that this is not an effective particle signal.Based on effective district determining unit of the present embodiment, utilize the pulse similarity rule of same particle synchronization to determine effective data field, the flow process of its a kind of preferably specific embodiment as shown in figure 12, comprises the following steps:
Step 120, to find that single reference channel of pulse is as by than reference channel one by one, data area outside the invalid data district being adopted the scheme of embodiment one to determine by the removing than reference channel is called initial valid data district, using each pulse window in initial effectively district as one by than time zone, obtain other each reference channel by than the threshold point in time zone.
Step 121, judges whether the single of other reference channel is equaled 0 by the quantity than the threshold point in time zone, if so, then thinks that this is by more successful than the pulse combined in time zone, otherwise performs step 122.
Step 122, judges whether list is equaled 2 by the quantity than the threshold point in time zone, if be not equal to 2, then thinks that this is by more failed than the pulse combined in time zone, if equal 2, then performs following steps;
Step 123, judges whether list is later than the time of rear threshold point than the time of front threshold point in the threshold point of two in time zone, if so, then thinks that this is by more failed than the pulse combined in time zone, otherwise performs following steps;
Step 124, judge whether the same of all reference channels is greater than interval threshold by than the difference between the peak value of pulse moment in time zone, if so, then think that this is by more failed than the pulse combined in time zone, otherwise think that this is by more successful than the pulse combined in time zone.
Can using successful for pulse combined pulse window region as valid data district, valid data district is the superposition of the time zone of the successful all respective pulses windows of pulse combined.Also invalid data district can be thought in the region of pulse combined failure, in initial valid data district, remove the invalid data district newly determined in the present embodiment again, the valid data district that remaining is for pulse recognition computing.Such as:
Above determining step is refined as and within the pulse window time period of any one reference channel, other reference channels is identified, judge what time as follows:
A) pulse of other reference channels only has a threshold point, situation as corresponding in P2 in passage in Figure 13 1;
B) there are more than 3 threshold point in the pulse of other reference channels, situation as corresponding in P1 in passage in Figure 13 1;
Although c) other reference channel pulses have two threshold point, its " front threshold point " time is later than " rear threshold point ", situation as corresponding in P3 in passage in Figure 13 1;
If find any one in above three kinds of situations, then determine invalid data district according to " laterally polluting " and " longitudinally polluting ".If do not have any one situation in above three kinds of situations to occur, in the pulse window time period of namely any one reference channel, other reference channels all only have 2 orders normal front and back threshold point (before rear threshold point moment > threshold point moment) or 0 threshold point, then further comprising the steps:
By overlapping for all pulse windows in the valid data district of each reference channel.Because a particle produces pulse at multiple passage, although the moment is close, but there is little difference unavoidably, and the pulse window on multiple passage also has difference unavoidably in state pause judgments moment time, by getting the mode of " union " in the present embodiment, obtain moment T1 and the latest moment T2 the earliest in all pulse windows, form the new time period, as shown in figure 14, this time period covers all pulse windows in the valid data district of all reference channels, namely " overlap ".
The peak value moment (moment corresponding for the mid point of threshold window can be considered as peak value moment) of the pulse between multiple reference channel is compared, if the maximum deviation of peak value is greater than certain value (a value shown in Figure 14, a value can set according to actual conditions), then all coherent pulse window regions are all " invalid data district " according to " laterally pollute " and " longitudinally polluting " rule settings.
After determining valid data district, within the valid data district T1-T2 time period shown in Figure 14, in each reference channel, according to " finding that pulse is then 1, the rule of " not finding that pulse is then 0 "; be " 1 " or " 0 " at each reference channel by its pulse validity Chx_Valid assignment; regular according to "AND"/inclusive-OR operation that user is arranged, the Chx_Valid of each reference channel being carried out logical operation and obtain the final validity result ChAll_Valid of many reference channels.If net result ChAll_Valid is " 0 ", then the data of all passages between T1-T2 are set in " invalid data district ", otherwise identify further.
If ChAll_Valid is " 1 ", then within the T1-T2 time period shown in Figure 14, identifies A, H, W information of all reference channels, non-reference passage and export.Conventional method is: the T1-T2 time period interscan maximal value shown in Figure 14, as peak value of pulse H, is added a little the integral result that the obtains A as pulse, A divided by the result of H as the W of pulse.Like this, A, H, W information of all passages identifies the most at last.
The present embodiment is after determining invalid data district, the valid data district that the effective impulse adopting similarity rule to determine that same particle produces further again in initial valid data district is corresponding, eliminate the data that some do not need to participate in pulse recognition further, thus further reduce data operation quantity and the accuracy that improve result of calculation.
It will be appreciated by those skilled in the art that, in above-mentioned embodiment, all or part of step of various method can be carried out instruction related hardware by program and completes, this program can be stored in a computer-readable recording medium, and storage medium can comprise: ROM (read-only memory), random access memory, disk or CD etc.
Above content is in conjunction with concrete embodiment further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, some simple deduction or replace can also be made.
Claims (17)
1. more than reference channel pulse recognition method, is characterized in that comprising:
The signal gathering the output of multiple reference channel obtains the pending data of each reference channel, and the signal that described reference channel exports is the electric signal that the optical information sent after the tested particle collected by reference channel is optically excited converts to;
Based on the pending data of each single reference channel with whether find pulse with this reference channel characteristic of correspondence threshold decision, described characteristic threshold value is the threshold value of the feature of interest of particle of interest, and described discovery pulse refers to and detects that peak value of pulse is greater than the pulse of characteristic threshold value;
For the single reference channel finding pulse, determine the invalid data district in the pending data of this single reference channel based on the pulse to be identified found;
According to the beginning and ending time in the invalid data district of the unified multiple reference channel in the invalid data district of each single reference channel;
The valid data district of multiple reference channel is determined based on the invalid data district after the unified beginning and ending time;
When carrying out pulse recognition, pulse recognition is carried out to the pulse in valid data district, no longer pulse recognition is carried out to the pulse in invalid data district.
2. the method for claim 1, is characterized in that, the described pulse to be identified based on finding determines that the invalid data district in the pending data of this single reference channel comprises:
Determine the pulse window of pulse to be identified, described pulse window is the region of pulse continuity;
Judge that whether the pulse window of current pulse to be identified is overlapping with the pulse window of pulse to be identified adjacent before and after it, if so, then will relate to overlapping pulse window region as invalid data district;
Judge that the pulse width of pulse to be identified is whether abnormal, if so, then using the pulse window region of the pulse to be identified of this exception as invalid data district.
3. method as claimed in claim 2, is characterized in that, describedly determines that the pulse window of pulse to be identified comprises:
The threshold window of feature based threshold test pulse to be identified, described threshold window is the pulse area between pulse to be identified two threshold point crossing with the boundary that characteristic threshold value is formed;
Threshold window is obtained pulse window respectively to after front and rear expansion presumptive area.
4. method as claimed in claim 2, is characterized in that, the beginning and ending time according to the invalid data district of the unified multiple reference channel in the invalid data district of each single reference channel comprises:
Circulation performs following steps, until the invalid data district of each single reference channel has the unified beginning and ending time:
Longitudinally pollute step, one by one single reference channel with invalid data district is compared as by than reference channel with other reference channel, using in the pending data area of other reference channel with by identical time period beginning and ending time in the invalid data district than reference channel as invalid data district;
Laterally pollute step, judge whether the invalid data district of each single reference channel pulse window adjacent with front and back has overlapping, if had, then relates to the pulse window region of overlap also as invalid data district.
5. the method according to any one of Claims 1-4, is characterized in that, determines that the valid data district of multiple reference channel comprises based on the invalid data district after the unified beginning and ending time:
Similarity rule between the pulse formed at multiple reference channel according to same particle, pulse combined is carried out to the data area except invalid data district of multiple reference channel, determine the valid data district of multiple reference channel, described valid data district at least comprises the successful pulse window region of pulse combined.
6. method as claimed in claim 5, it is characterized in that, described similarity rule comprises: the pulse in the respective pulses window of multiple reference channel is no more than 1, and described pulse combined step comprises:
To find that single reference channel of pulse is as by than reference channel one by one, using by than the pulse window in the data area except invalid data district of reference channel as by than time zone, obtain other each reference channel by than the threshold point in time zone;
Judge whether the single of other reference channel is equaled 0 by the quantity than the threshold point in time zone, if so, then think that this is by more successful than the pulse combined in time zone, otherwise perform following steps;
Judge whether list is equaled 2 by the quantity than the threshold point in time zone, if be not equal to 2, then think that this is by more failed than the pulse combined in time zone, if equal 2, then performs following steps;
Judge single by the time than front threshold point in the threshold point of two in time zone whether prior to the time of rear threshold point, if so, then think that this is by than the pulse combined success in time zone, otherwise think that this is by than the pulse combined failure in time zone.
7. method as claimed in claim 5, it is characterized in that, described similarity rule comprises: the pulse in the respective pulses window of multiple reference channel is no more than 1 and peak value moment is close, and described pulse combined step comprises:
To find that single reference channel of pulse is as by than reference channel one by one, using by than each pulse window in the data area except invalid data district of reference channel as one by than time zone, obtain other each reference channel by than the threshold point in time zone;
Judge whether the single of other reference channel is equaled 0 by the quantity than the threshold point in time zone, if so, then think that this is by more successful than the pulse combined in time zone, otherwise perform following steps;
Judge whether list is equaled 2 by the quantity than the threshold point in time zone, if be not equal to 2, then think that this is by more failed than the pulse combined in time zone, if equal 2, then performs following steps;
Judge whether list is later than the time of rear threshold point than the time of front threshold point in the threshold point of two in time zone, if so, then think that this is by more failed than the pulse combined in time zone, otherwise perform following steps;
Judge whether the same of all reference channels is greater than interval threshold by than the difference between the peak value of pulse moment in time zone, if, then think that this is by more failed than the pulse combined in time zone, otherwise think that this is by more successful than the pulse combined in time zone.
8. method as claimed in claim 5, it is characterized in that, described valid data district is the superposition of the time zone of the successful all respective pulses windows of pulse combined.
9. the method according to any one of claim 1 to 8, is characterized in that, also comprises after determining the valid data district of multiple reference channel:
Whether find that the logical value of each reference channel is determined in pulse according to each reference channel;
Logical operation is carried out based on the logical value of each reference channel and logical algorithm;
Determine whether to carry out pulse recognition to the pending data of all passages according to logic operation result.
10. more than reference channel pulse recognition device, is characterized in that comprising:
Data acquisition unit, the signal exported for gathering multiple reference channel obtains the pending data of each reference channel, and the signal that described reference channel exports is the electric signal that the optical information sent after the tested particle collected by reference channel is optically excited converts to;
Pulse finds unit, for the pending data based on each single reference channel with whether find pulse with this reference channel characteristic of correspondence threshold decision, described characteristic threshold value is the threshold value of the feature of interest of particle of interest, and described discovery pulse refers to and detects that peak value of pulse is greater than the pulse of characteristic threshold value;
Single channel dead space determining unit, for for the single reference channel finding pulse, determines the invalid data district in the pending data of this single reference channel based on the pulse to be identified found;
Dead space Unified Element, for the beginning and ending time in the invalid data district according to the unified multiple reference channel in the invalid data district of each single reference channel;
Effective district determining unit, determines the valid data district of multiple reference channel based on the invalid data district after the unified beginning and ending time;
Pulse recognition unit, for carrying out pulse recognition when carrying out pulse recognition to the pulse in valid data district, no longer carries out pulse recognition to the pulse in invalid data district.
11. devices as claimed in claim 10, is characterized in that, described single channel dead space determining unit comprises:
Pulse window determination subelement, for determining the pulse window of pulse to be identified, described pulse window is the region of pulse continuity;
First judgment sub-unit, for judging that whether the pulse window of current pulse to be identified is overlapping with the pulse window of pulse to be identified adjacent before and after it, if so, then will relate to overlapping pulse window region as invalid data district;
Second judgment sub-unit, whether abnormal for judging the pulse width of pulse to be identified, if so, then using the pulse window region of the pulse to be identified of this exception as invalid data district.
12. devices as claimed in claim 11, it is characterized in that, dead space Unified Element comprises:
Longitudinally pollute subelement, for one by one single reference channel with invalid data district being compared as by than reference channel with other reference channel, using in the pending data area of other reference channel with by identical time period beginning and ending time in the invalid data district than reference channel as invalid data district;
Laterally pollute subelement, for judging whether the invalid data district of each single reference channel pulse window adjacent with front and back has overlapping, if had, then relates to the pulse window region of overlap also as invalid data district;
3rd judgment sub-unit, for judging whether the invalid data district of each single reference channel has the unified beginning and ending time, determines to work on or end loop by longitudinally polluting subelement and laterally polluting subelement according to judged result.
13. devices according to any one of claim 10 to 12, it is characterized in that, effective district determining unit is used for the similarity rule between the pulse that formed at multiple reference channel according to same particle, by carrying out pulse combined to the data area except invalid data district of multiple reference channel, determine the valid data district of multiple reference channel according to pulse combined result, described valid data district at least comprises the successful pulse window region of pulse combined.
14. devices as claimed in claim 13, it is characterized in that, described similarity rule comprises: the pulse in the respective pulses window of multiple reference channel is no more than 1, to find that single reference channel of pulse is as by than reference channel one by one when pulse combined, using by than the pulse window in the data area except invalid data district of reference channel as by than time zone, described effective district determining unit comprises:
Threshold point obtains subelement, for obtain other each reference channel by than the threshold point in time zone;
4th judgment sub-unit, for judging whether the single of other reference channel is equaled 0 by the quantity than the threshold point in time zone, if so, then thinks that this is by more successful than the pulse combined in time zone;
5th judgment sub-unit, for when the quantity of the 4th judgment sub-unit judgment threshold point is not equal to 0, judges whether list is equaled 2 by the quantity than the threshold point in time zone, if be not equal to 2, then thinks that this is by more failed than the pulse combined in time zone;
6th judgment sub-unit, for single equaled 2 by the quantity than the threshold point in time zone time judge that the time of front threshold point in these two threshold point is whether prior to the time of rear threshold point, if, then think that this is by more successful than the pulse combined in time zone, otherwise think that this is by more failed than the pulse combined in time zone.
15. devices as claimed in claim 13, it is characterized in that, described similarity rule comprises: the pulse in the respective pulses window of multiple reference channel is no more than 1 and peak value moment is close, to find that single reference channel of pulse is as by than reference channel one by one when pulse combined, using by than the pulse window in the data area except invalid data district of reference channel as by than time zone, described effective district determining unit comprises:
Threshold point obtains subelement, for obtain other each reference channel by than the threshold point in time zone;
4th judgment sub-unit, for judging whether the single of other reference channel is equaled 0 by the quantity than the threshold point in time zone, if so, then thinks that this is by more successful than the pulse combined in time zone;
5th judgment sub-unit, for when the quantity of the 4th judgment sub-unit judgment threshold point is not equal to 0, judges whether list is equaled 2 by the quantity than the threshold point in time zone, if be not equal to 2, then thinks that this is by more failed than the pulse combined in time zone;
6th judgment sub-unit, for judge in the 5th judgment sub-unit single equaled 2 by the quantity than the threshold point in time zone time judge that the time of front threshold point in these two threshold point is whether prior to the time of rear threshold point, if the time of front threshold point is later than the time of rear threshold point, then think that this is by more failed than the pulse combined in time zone;
7th judgment sub-unit, during for the time of time of threshold point before the 6th judgment sub-unit judges prior to rear threshold point, judge whether the same of all reference channels is greater than interval threshold by than the difference between the peak value of pulse moment in time zone, if, then think that this is by more failed than the pulse combined in time zone, otherwise think that this is by more successful than the pulse combined in time zone.
16. devices as claimed in claim 13, is characterized in that, described valid data district is the superposition of the time zone of the successful all respective pulses windows of pulse combined.
17. 1 kinds of particle analyzers, is characterized in that comprising:
Excitation source, for providing the exciting light irradiating tested particle;
Flow chamber, for providing tested particle irradiated place;
Light collecting device, for collecting the optical information sent after tested particle is optically excited, described light collecting device comprises multiple reference channel; With
Device according to any one of claim 10-16.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310754674.6A CN104749086B (en) | 2013-12-31 | 2013-12-31 | More reference channel pulse recognition method, apparatus and particle analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310754674.6A CN104749086B (en) | 2013-12-31 | 2013-12-31 | More reference channel pulse recognition method, apparatus and particle analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104749086A true CN104749086A (en) | 2015-07-01 |
| CN104749086B CN104749086B (en) | 2018-12-18 |
Family
ID=53589099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310754674.6A Active CN104749086B (en) | 2013-12-31 | 2013-12-31 | More reference channel pulse recognition method, apparatus and particle analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104749086B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107101983A (en) * | 2017-04-25 | 2017-08-29 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Droplet multichannel fluorescence synchronous waveform method and device |
| CN107290253A (en) * | 2017-06-27 | 2017-10-24 | 四川迈克生物医疗电子有限公司 | The processing method and processing device of pattern detection data |
| CN108027362A (en) * | 2015-09-15 | 2018-05-11 | 生物辐射实验室股份有限公司 | Threshold selector for flow cytometer |
| JP2019502915A (en) * | 2015-12-18 | 2019-01-31 | アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ | Particle detection method and system for implementing the same |
| CN111235023A (en) * | 2020-03-25 | 2020-06-05 | 梨薯(厦门)科技有限公司 | Single particle detection method based on multi-channel triggering |
| CN112881351A (en) * | 2019-11-30 | 2021-06-01 | 深圳市帝迈生物技术有限公司 | Particle detection method and device based on pulse recognition and storage medium |
| CN115015089A (en) * | 2022-08-08 | 2022-09-06 | 深圳市帝迈生物技术有限公司 | Stream type detection device and method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4240029A (en) * | 1979-04-20 | 1980-12-16 | Becton Dickinson & Company | Apparatus for counting particles in a liquid suspension |
| US4375615A (en) * | 1979-04-20 | 1983-03-01 | Becton Dickinson And Company | Apparatus for counting particles in a liquid suspension |
| US4412175A (en) * | 1981-04-30 | 1983-10-25 | Coulter Electronics, Inc. | Debris alarm |
| WO2009093017A1 (en) * | 2008-01-25 | 2009-07-30 | The Secretary Of State For Defence | Fluid-borne particle detector |
| CN101501472A (en) * | 2006-08-17 | 2009-08-05 | 科安比司特公司 | Blood processing apparatus with robust automated process control |
| CN102818761A (en) * | 2003-03-28 | 2012-12-12 | 英格朗公司 | Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm |
-
2013
- 2013-12-31 CN CN201310754674.6A patent/CN104749086B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4240029A (en) * | 1979-04-20 | 1980-12-16 | Becton Dickinson & Company | Apparatus for counting particles in a liquid suspension |
| US4375615A (en) * | 1979-04-20 | 1983-03-01 | Becton Dickinson And Company | Apparatus for counting particles in a liquid suspension |
| US4412175A (en) * | 1981-04-30 | 1983-10-25 | Coulter Electronics, Inc. | Debris alarm |
| CN102818761A (en) * | 2003-03-28 | 2012-12-12 | 英格朗公司 | Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm |
| CN101501472A (en) * | 2006-08-17 | 2009-08-05 | 科安比司特公司 | Blood processing apparatus with robust automated process control |
| WO2009093017A1 (en) * | 2008-01-25 | 2009-07-30 | The Secretary Of State For Defence | Fluid-borne particle detector |
Non-Patent Citations (3)
| Title |
|---|
| MANABU TAKAHASHI: "A Multichannel Method for the Analysis of DNA Content Distribution Using Flow Cytometric Data", 《COMPUTERS AND BIOMEDICAL RESEARCH》 * |
| MARIA A SUNI ET AL.: "Performance of plate-based cytokine flow cytometry with automated data analysis", 《BMC IMMUNOLOGY》 * |
| 张卫杰: "高速大容量数据采集系统设计与实现", 《电子测量与仪器学报》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108027362A (en) * | 2015-09-15 | 2018-05-11 | 生物辐射实验室股份有限公司 | Threshold selector for flow cytometer |
| CN108027362B (en) * | 2015-09-15 | 2020-05-05 | 生物辐射实验室股份有限公司 | Threshold selector for flow cytometer |
| JP2019502915A (en) * | 2015-12-18 | 2019-01-31 | アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ | Particle detection method and system for implementing the same |
| CN107101983A (en) * | 2017-04-25 | 2017-08-29 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Droplet multichannel fluorescence synchronous waveform method and device |
| CN107101983B (en) * | 2017-04-25 | 2019-11-08 | 广东永诺医疗科技有限公司 | Droplet multichannel fluorescence synchronous waveform method and device |
| CN107290253A (en) * | 2017-06-27 | 2017-10-24 | 四川迈克生物医疗电子有限公司 | The processing method and processing device of pattern detection data |
| CN107290253B (en) * | 2017-06-27 | 2019-06-04 | 迈克医疗电子有限公司 | The processing method and processing device of pattern detection data |
| CN112881351A (en) * | 2019-11-30 | 2021-06-01 | 深圳市帝迈生物技术有限公司 | Particle detection method and device based on pulse recognition and storage medium |
| CN111235023A (en) * | 2020-03-25 | 2020-06-05 | 梨薯(厦门)科技有限公司 | Single particle detection method based on multi-channel triggering |
| CN115015089A (en) * | 2022-08-08 | 2022-09-06 | 深圳市帝迈生物技术有限公司 | Stream type detection device and method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104749086B (en) | 2018-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104749086A (en) | Multi-reference-channel pulse recognition method and device, and particle analyzer | |
| US10258250B2 (en) | GPU-based parallel electrocardiogram signal analysis method, computer readable storage medium and device | |
| CN102319063B (en) | Device and method for improving acceleration identification accuracy of fetal heart rate data | |
| CN103720468B (en) | Be applied to artifact identification method and the device of dynamic electrocardiogram (ECG) data | |
| CN102121892B (en) | Sample analyzer and sample analyzing method | |
| US11944470B2 (en) | Method and device for sampling a pulse signal, and computer program medium | |
| CN108511055B (en) | Ventricular premature beat recognition system and method based on classifier fusion and diagnosis rules | |
| Thompson et al. | The mean–variance relationship reveals two possible strategies for dynamic brain connectivity analysis in fMRI | |
| CN102779234A (en) | Electrocardiogram classified processing method and device | |
| Yao et al. | Automated retinal blood vessels segmentation based on simplified PCNN and fast 2D-Otsu algorithm | |
| CN102693361A (en) | Drawing method for trend curve of large dataset | |
| CN110680302A (en) | Automatic identification method for electrocardiosignal characteristic wave | |
| CN103892830A (en) | Emotion detection method and system based on human skin resistance changes | |
| CN103549948A (en) | Cardiac activation time detection | |
| CN104323771A (en) | Method and device for detecting P-wave and T-wave in electrocardiogram (ECG) signal | |
| CN104462979A (en) | Automatic dynamic detection method and device of application program | |
| CN104297213A (en) | Blood cell analyzing instrument and recognizing method and system for abnormal cells thereof | |
| CN107625527A (en) | A kind of lie detecting method and device | |
| JP6060793B2 (en) | Peak detector | |
| CN109394248B (en) | Driving fatigue detection method and system | |
| CN109567869B (en) | Method and system for processing accelerated activities on fetal heart rate curve | |
| CN107610753A (en) | One kind meets event determination method and device | |
| CN106770151A (en) | A kind of method and device of quick detection raman spectral signal | |
| CN112603334B (en) | Spike detection method based on time sequence characteristics and stacked Bi-LSTM network | |
| Zhou et al. | A hybrid expert system for individualized quantification of electrical status epilepticus during sleep using biogeography-based optimization |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |