CN104741158A - Device and method for generating microdroplets by inertial force - Google Patents
Device and method for generating microdroplets by inertial force Download PDFInfo
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Abstract
The invention discloses a device and a method for generating microdroplets by inertial force. A device main body comprises an acceleration generation device, first liquid, a droplet forming device and a collecting device, wherein the acceleration generation device is used for generating centripetal acceleration; the droplet forming device is provided with a microchannel; the first liquid generates droplets with the diameter being 5-300mu m by the microchannel of the droplet forming device under the action of the acceleration generation device; the collecting device is used for collecting the generated droplets; and the surface of the first liquid at the outlet part of the droplet forming device is in a hydrophobic state. The new generation method disclosed by the invention has the advantages that the defects of the traditional method are overcome, no liquid is left on the surface, high-flux and fast generation of the microdroplets can be realized, and the controllable dispersion of the droplets can be realized.
Description
Technical field
The present invention relates to biological technical field, more specifically relate to a kind of generation device and method of microlayer model, and application.
Technical background
In recent years, biotech development proposes more and more higher requirement to droplet treatment technology: first aspect, the low loss demand of sample.Microsequencing sample is as unicellular sample (as embryonated egg), and section sample, in the preparation of the rare samples such as cancer (as CTC cell) sample, characterization technique, keeps the integrality of biological sample to be even more important.And for other digital quantitative technology such as digital PCR, digital MDA, keep the integrality of sample to reach the necessary condition of more than 99% technology especially, namely need to ensure as far as possible few sample loss in sample treatment, testing process.But use the droplet treatment technology of traditional microfluidic chip solution, the unavoidable droplet treatment medium that produces remains, and cause the nucleic acid in sample, albumen waits for and analyzes component loss.
Second aspect, uniformity demands.Prepared by these precisions for micro-example, detection technique, needs again the droplet size of generation even, can not cross big-difference again, cause bringing tendentiousness and uncertainty in subsequent experimental.
The third aspect, high flux demand.Along with being on the increase of upstream sample number, the demand that extensive sample is prepared simultaneously is constantly increased.Existing micro flow chip technology generates the technology of drop, and every platform instrument at most only can make 8 samples droplet treatment simultaneously, and due to the pipe design that it is loaded down with trivial details, fluid control design case, can not meet expansion scale, the needs of flux.
Fourth aspect, refrigeration demand.Biological sample, especially DNA, RNA, protein sample is more fragile.Further, after some reactant liquor configures, just can react (as MDA) under normal temperature, need (< 10min) fast to produce drop at low temperature (being less than 10 degrees Celsius, preferably at 0-4 degree Celsius).Conventional method can not be lowered the temperature easily to micro flow chip, and it is also fast not to produce speed.
When liquid is very small time, it shows characteristic distinct with macroscopical liquid.In such as face size 10 × 10 μm of microchannels, fluid is in laminar condition, heat transfer and mass transfer all with the macrofluid of routine, difference in the rubber tube of such as common lab beaker or 1 centimetre of end face.All the time, fast the accurate drop producing micron level is a huge engineering challenge.Micro-fluidic chip can produce the drop of controlled diameter fast, but its equipment is complicated, and cost is higher.Ink-jet technology can produce drop at a high speed, but its liquid-drop diameter uniformity is bad, and needs ink discharge device, is not suitable for common Biochemistry Experiment room.Therefore, development produces uniform droplet easily fast, such as less than 300 microns, has very important Biochemical Significance.
The technology of the drop based on high inertia force generation provided by the invention, well meets above-mentioned 4 demands.1) noresidue.Whole drop, in the high centrifugal force field of centrifuge, completely via droplet treatment mouth, produces drop, can not produce residual.2) controlled single dispersing.The equilibrium state that whole drop produces drop is all identical, so drop size is accurately identical, and reproducible, controlled range is large, does not have the impacts such as liquid turbulence.3) high flux.Drop can be produced owing to only needing centrifugal force, only to need on a conventional table centrifuge (as thermoPICO17, PICO 21, eppendorf 5430R etc.), 1.5mL centrifuge tube is used to collect the droplet treatment that just simultaneously can make 24 samples, if use the form of 8 townhouse pipes, 96 orifice plates, 384 orifice plates, even 1536 orifice plates further, and in addition stacking, be easy to use one desk centrifuge, realize the droplet treatment of several thousand individual independently samples even up to ten thousand simultaneously.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, propose the large aqueous phase droplets generation new methods such as one is simple.Utilize inertia force to drive droplet treatment medium, through microchannel, rupture behind the empty road junction of outflow, produce drop, and drop in trapping medium.Can be air or low-density out-phase with producing the liquid of drop contact.
The fracture of drop under inertia force condition, meets a clear and definite physical process: when terrestrial gravitation suffered by drop is greater than the surface tension of most weakness, drop ruptures.Utilize the inertia force constant determined, this principle is generally applied in be measured on solution surface tension, is called drip method, and size is not by 0.5-2mm etc.And use inertia force and the adjustment tension force of change, come to produce small size, the drop of 5-200 μm of diameter at a high speed, not only make use of capillary principle, have more standby wide Biochemical Significance.Uniform fine droplet, in biological or chemical context of detection, has possessed huge potentiality.
In the present invention, alleged acceleration refers under the effect of extraneous non-natural power, such as centripetal force, tractive force, makes liquid be subject to the contrary power in acceleration direction, thus makes the oriented trend in contrast to the motion of acceleration direction of liquid.In the present invention, first liquid is subject to the effect of inertia force, forms drop by droplet treatment device.First liquid is also referred to as droplet treatment medium.
The generation device of a kind of drop of the present invention, is characterized in that, comprise acceleration generation device, it is for generation of centripetal acceleration; Comprise first liquid; Comprise droplet treatment device, it possesses microchannel, and first liquid produces the drop of 5-300 μm of diameter under the effect of acceleration generation device by the microchannel of droplet treatment device; Comprise gathering-device, it is for collecting the drop of generation; Wherein, first liquid is hydrophobic state on the surface of droplet treatment device exit portion.The internal diameter of wherein said microchannel is 1-100 μm, and length is 0.1-10mm.
The invention provides a kind of production method of drop, it is characterized in that, comprise acceleration generation device, it is for generation of centripetal acceleration; Comprise first liquid; Comprise droplet treatment device, it possesses microchannel, and first liquid produces the drop of 5-300 μm of diameter under the effect of acceleration generation device by the microchannel of droplet treatment device; Comprise gathering-device, it is for collecting the drop of generation; Wherein, first liquid is hydrophobic state on the surface of droplet treatment device exit portion.The internal diameter of wherein said microchannel is 1-100 μm, and length is 0.1-10mm.
Wherein first liquid refers to common low viscous liquid or solution.Such as first liquid is the solution of the materials such as water, cell culture fluid, ethanol, or comprises the combination of cell, DNA, RNA or protein in liquid.
Droplet treatment device is the device of band microchannel.Such as common capillary, narrow meshed film or narrow meshed plate; The internal diameter of capillary or the diameter of aperture are 1-100 μm; The material of droplet treatment device can be inorganic material as glass, quartz, silicon; Or common organic plastic material is as polystyrene, Merlon, polymethylacrylic acid, polyamide, polyethylene, polyvinyl chloride, polypropylene; Fluoro-containing plastic as polytetrafluoroethylene (PTFE), PVDF etc.
According to further preferred technical scheme, described aperture is circular hole or polygonal hole; Internal diameter i.e. its length of side of described square hole is 1-100 μm.
According to further preferred technical scheme, the diameter of described drop is 10-250 μm, preferred 20-200 micron; The internal diameter of described microchannel is 2-90 micron, preferred 5-50 micron; The length of microchannel is 0.5-6mm, preferred 1-5mm.
According to further preferred technical scheme, droplet treatment device is 1,2 ... ..n, wherein n be greater than 2 integer.
According to further preferred technical scheme, gathering-device is collected by centrifugation pipe.
According to further preferred technical scheme, further can also comprise the storage device of first liquid, it is for storing first liquid.
Further can also comprise second liquid, be positioned at gathering-device, for collecting the drop of the first liquid of generation, the part of the outlet of described droplet treatment device does not contact with second liquid.First liquid does not contact with second liquid with changing in drop before changing into drop.
According to further preferred technical scheme, described acceleration generation device is centrifuge.
According to further preferred technical scheme, described acceleration can be constant or change.
According to further preferred technical scheme, described microchannel is straight or bending.
According to further preferred technical scheme, droplet treatment device is tube or the cone that there is opening front end.
According to further preferred technical scheme, further comprise delay medium or deferred mount.
According to further preferred technical scheme, droplet treatment device and storage device are an entirety.
According to further preferred technical scheme, droplet treatment device materials is inorganic material, organic material or composite.Inorganic material is glass, quartz, pottery, the one in metal.Organic material is polystyrene, polyether-ether-ketone, the one in polyester macromolecule material.Composite is the mixture of organic material described in one or more and/or inorganic material.
According to further preferred technical scheme, described droplet treatment device whole or only exit portion, through hydrophobic and/or Hydrophilic Surface Treatment, make first liquid be hydrophobic state on the surface of droplet treatment device exit portion; Surface-treated method has: directly cover painting, and chemical reaction is modified, chemical deposition etc.
According to demand, can not comprise the storage device of first liquid, such as utilize capillary as droplet treatment device, capillary inherently can store a certain amount of liquid, as shown in Figure 1.According to demand, further can comprise the storage device of first liquid, it can be the material different with droplet treatment device; Also can be the material identical with droplet treatment device, and an entirety can be formed with droplet treatment device.
Second liquid is also referred to as trapping medium, and it mainly contains following effect:
(1) do not dissolve each other with aqueous phase.
(2) provide buoyancy, significantly reduce the gravity that drop receives, keep the drop produced not because centrifugal force breaks, merge.
(3) provide viscosity, reduce drop decrease speed, buffering drop, avoid merging.
There is provided activating agent, stable droplet.
(4) density is less than droplet treatment phase, and aqueous phase droplets can be allowed to be deposited to bottom trapping medium, avoids the dripping drops newly produced with top to collide, and produces and merges.
(5) prevent moisture from evaporating.In subsequent experimental, as prevented moisture from evaporating in PCR process
(6) as image forming medium, subsequent analysis, the mobile phase of fluorescent collecting etc.
Produce device and the droplet treatment device of drop, the simplest droplet treatment device is capillary.Capillary can be made up of one or many.Can be made up of a capillary or capillary of following morely, also can adopt porous capillary or perforated membrane, porous plate, as shown in Figure 2.
The manufacture of tubule by machining, can draw after machining, wet etching, the techniques such as dry etching, and supporting have the operation such as cutting, polishing to complete.
Tubule materials can be inorganic material as glass, quartz, silicon; Or common organic plastic material is as polystyrene, Merlon, polymethylacrylic acid, polyamide, polyethylene, polyvinyl chloride, polypropylene; Fluoro-containing plastic as polytetrafluoroethylene (PTFE), PVDF etc.
Tubule or tubule group surface need to modify, and the result of modification should make droplet treatment medium be unlikely to spread out at the microtubule mouth of pipe.Droplet treatment medium should be greater than 90 degree with the contact angle on surface.
This device overall structure is divided into three parts.Droplet treatment device (on), collecting pipe (under) two parts composition; Droplet treatment pipe comprises drop and holds district and microtubule district two parts.
The effect of droplet treatment device holds droplet treatment medium temporarily and utilizes microcosmic and high inertia force to produce drop.Its surface should have low absorption left and right to held droplet treatment medium (being generally aqueous phase) and solute.Droplet treatment pipe is microtubule district bottom, and microtubule district and holding between district seals completely, can bonding or integrated manufacture, and make under inertia force effect, all droplet treatment media all could arrive in collecting pipe through microtubule.
Collecting pipe can be conventional centrifuge tube or orifice plate, or collecting pipe supporting specially.Hold trapping medium in collecting pipe, be generally inert organic solvents, and density is less than droplet treatment medium.Trapping medium page height must not be too high, can not contact lower end, microtubule district; Trapping medium can not be very few, must effectively collect the drop fallen.Usual trapping medium is more than 10 times of droplet treatment medium total amount.
Inertia force makes first liquid produce the power of microlayer model by the microchannel of droplet treatment device.Common inertia force has a variety of, such as can the hand-held described droplet treatment device of people and collecting pipe, and namely whipping can impel liquid to produce drop by the microchannel of certain size back and forth, but it can not produce uniform drop, can not meet actual application.Droplet treatment device can be contained in the bottom of rocking equipment along the centripetal direction of rocking equipment.Whirligig, such as centrifuge can provide stable inertia force.
Delay start designs.Because in accelerator, inertia force does not reach balance, and the drop of generation is uneven.In order to complete makes the drop that whole droplet treatment medium generation etc. is large, liquid must do not produced in the accelerated period of acceleration generation device.Implementation has two classes: 1, increases device, as the openable office of ability under certain inertia force, as sealed with wax; Or 2, increase a kind of high viscosity high density liquid and holding in pipe, this liquid first flows out from micropore, and really need the liquid of droplet treatment to be retained in top, blocked a period of time flows out again.Residence time is determined by the volume adding high density liquid.Retardance liquid should have following feature: droplet treatment medium of 1, getting along well is miscible or react, and has enough chemical inertnesses.2, the drop not producing drop or generation can not merge or produce drop can be distinguished and be separated, and does not participate in subsequent reactions.3, can be miscible with collection liquid.
The principle that drop produces can be explained by following calculating:
Critical state equation:
2πrγ＝Gm......(1)
γ: surface tension, unit (mN.m); R: microchannel diameter.Unit (m); G: centrifugal force value.Unit (N/m2); M: drop mass.
The density formula of liquid:
V: droplet size.R: liquid-drop diameter.ρ: drop density.
(2) bring in (1) and obtain:
But, if under given conditions, the inertia force that drop is subject to can be adjusted, just can be continuous, the drop that repeatedly generation etc. are large.Further, by adjustment: γ, G, r, ρ item, adjustable droplet size (V), thus the number of drops changing unit liquid volume.
The adjustment of surface tension γ.By adding ion, bioactive agent composition in aqueous phase, can γ be reduced, drop is diminished.Activating agent can be Span activating agent, Tween activating agent, triton activating agent, fluorine-containing surfactant, as EA activating agent, and perfluoro caprylic acid, perfluor acid amides etc.γ is proportional to V
The adjustment of G.Adjustment inertia force size, especially by adjustment centrifuge rotating speed, the size of adjustable G, G usually at 1,000-1,000,000m/s2 (0.1-100krcf).1/G is proportional to V.
The adjustment of r.Adjustment microchannel size, can change droplet size.Less microchannel diameter can produce less drop.R is proportional to V.
ρ fluid density adjusts.Such as increase glucose, the solutes such as glycerine, water density 1.0-1.2g/mL can be adjusted.1/ ρ is proportional to V.
It is adjustable that drop produces speed, because the restriction of revolution.Flow velocity, revolution, and flow resistance has following relationship.
The pressure difference p at its volume flow Q and pipe two ends, the relation of flow resistance R:
Q＝p/R
Pressure reduction p in inertia force G, the relation of page height h and fluid density ρ:
p＝ρGh
The flow resistance R of laminar flow round pipe liquid is calculated as follows:
In above formula: the radius r of microtubule, length L, and the coefficient of viscosity η (cst) of fluid.
By adjustment flow resistance R, acceleration G, liquid level.Just adjustable drop produces speed, thus changes experiment total time.
Adjustment L capillary pipe length.Design modifying.Optional from 10 μm of-10mm.
Adjustment r capillary radius.Different size is optional.From 1-100 μm.
The viscosity of adjustment η first liquid.Temperature is lower, and viscosity is larger.
The drop that the method for generation drop provided by the invention produces is even, and device is simple, can use to the equipment such as centrifuge by direct combination.By experiment, the discovery that we are surprised, if first liquid can not be hydrophobic state on the surface of droplet treatment device exit portion, then can not produce single dispersing drop.Time the outlet of droplet treatment device does not contact second liquid, the drop of generation has better uniformity.The drop uniformity produced is good, and can accomplish very high flux.
Accompanying drawing explanation
Fig. 1, the generation principle of drop.
Fig. 2, single dropproducing devices and multiple dropproducing devices schematic diagram.
Fig. 3, single integrated dropproducing devices and multiple integrated dropproducing devices schematic diagram.
Fig. 4, produces the result figure of drop.
Fig. 5, physics time-delay mechanism schematic diagram.
Specific embodiment:
Specific embodiment in the specific embodiment of the invention is only further illustrate for of the present invention, and the limiting factor of cost invention not.
Embodiment 1
Utilize eppendorf5430R centrifuge as acceleration generation device, utilize water as first liquid, utilize glass capillary as droplet treatment device, the exit trichlorine perfluoro silane of capillary does finishing; The storage device of droplet treatment pipe as first liquid of processing one and centrifuge tube coupling is passed through at the mouth of pipe place of centrifuge tube.Lid is added for sealing in the outside of droplet treatment pipe.First liquid is added droplet treatment pipe, be installed to after on centrifuge tube and load centrifuge.Utilize eppendorf 2mL collected by centrifugation pipe as gathering-device.Utilize 500 μ L mineral oil (adding tween80 and span80 as activating agent) as second liquid.The diameter distribution that can obtain the drop under different parameters is as shown in table 1.Droplet treatment device is as shown in Fig. 3 leftmost diagram.
The related table (γ=60mN/m) of table 1 rotating speed, microchannel size and microlayer model diameter
Embodiment 2.
Select capillary as droplet treatment device, the internal diameter of capillary is 10 microns, and length is 5 millimeters, utilizes thermo PICO17 centrifuge as inertia force generation device, gathering-device is collected by centrifugation pipe, and the exit trichlorine perfluoro silane of capillary does finishing.First liquid selects water, passes through the storage device of droplet treatment pipe as first liquid of processing one and centrifuge tube coupling at the mouth of pipe place of centrifuge tube.Lid is added for sealing in the outside of droplet treatment pipe.First liquid is added droplet treatment pipe, be installed to after on centrifuge tube and load centrifuge.The centrifugal force of centrifuge is set to 3KG, and the direction that centrifuge produces centripetal force is identical with the direction of capillary.
Utilize mineral oil as trapping medium.The diameter of the drop collected is 48 μm, and CV is 3%.The partial drop produced as shown in Figure 4.
Embodiment 3.
Other condition is identical with embodiment 2, and wherein the surface free of capillary crosses modification.The diameter producing drop is about 145 μm, and CV is 30%.
Embodiment 4.
Other condition is identical with embodiment 1, wherein, selects 96 hole PCR plate to be collected by centrifugation pipe, is furnished with 96 orifice plates of capillary as droplet treatment pipe with self-control,
Embodiment 5.
Other condition is identical with embodiment 1, and wherein, droplet treatment device is the capillary group of 100 capillary compositions.The diameter producing drop is 48 μm, and CV is 3%
Embodiment 6.
Other condition is identical with embodiment 1, and wherein, it possesses fluid collection device and collecting pipe, and possess the droplet treatment device of integration, i.e. droplet treatment pipe in figure, multiple outlet is arranged at the bottom of droplet treatment pipe.8 droplet treatment devices can form 8 townhouse forms, also can form 96 well format, as shown in Figure 3.
Embodiment 7.
Other condition is identical with embodiment 1, and wherein, delayed startup device as shown in Figure 5, will inject 100uL trapping medium mineral oil in collecting pipe below.Pipe will be held be inserted in collecting pipe.High viscosity fluorocarbon oil (as Dupont GPL100 series and VPF series fluorocarbon oil) 3uL is injected and holds pipe, then the injection of 20uL droplet treatment medium is held pipe.Cover lid.
Embodiment 8.
Other condition is identical with embodiment 1, and wherein, the outlet of droplet treatment device is positioned under the liquid level of second liquid, and that is first liquid directly contacts second liquid after leaving outlet.The liquid-drop diameter produced is about 120 microns, and CV is 15%.
Claims (32)
1. a generation device for drop, is characterized in that, comprise acceleration generation device, it is for generation of centripetal acceleration; First liquid; Droplet treatment device, it possesses microchannel, and first liquid produces the drop of 5-300 μm of diameter under the effect of acceleration generation device by the microchannel of droplet treatment device; Gathering-device, it is for collecting the drop of generation; Wherein, first liquid is hydrophobic state on the surface of droplet treatment device exit portion.The internal diameter of wherein said microchannel is 1-100 μm, and length is 0.1-10mm.
2. dropproducing devices according to claim 1, is characterized in that,
First liquid is the solution of the materials such as water, cell culture fluid, ethanol, or comprises the combination of cell, DNA, RNA or protein in liquid.
3. dropproducing devices according to claim 1, is characterized in that,
Droplet treatment device is the device of band microchannel, is selected from capillary, narrow meshed film or narrow meshed plate; The internal diameter of capillary or the diameter of aperture are 1-100 μm; The material of droplet treatment device can be inorganic material as glass, quartz, silicon; Or common organic plastic material is as polystyrene, Merlon, polymethylacrylic acid, polyamide, polyethylene, polyvinyl chloride, polypropylene; Fluoro-containing plastic as polytetrafluoroethylene (PTFE), PVDF etc.
4. dropproducing devices according to claim 3, is characterized in that,
Described aperture is circular hole or polygonal hole; Internal diameter i.e. its length of side of described polygonal hole is 1-100 μm.
5. dropproducing devices according to claim 1, is characterized in that,
The diameter of described drop is 10-250 μm, preferred 20-200 micron; The internal diameter of described microchannel is 2-90 micron, preferred 5-50 micron; The length of microchannel is 0.5-6mm, preferred 1-5mm.
6. dropproducing devices according to claim 1, is characterized in that,
Droplet treatment device is 1,2 ... ..n, wherein n be greater than 2 integer.
7. dropproducing devices according to claim 1, is characterized in that,
Gathering-device is collected by centrifugation pipe.
8. dropproducing devices according to claim 1, is characterized in that,
Further comprise the storage device of first liquid, it is for storing first liquid.
Further comprise second liquid, be positioned at gathering-device, for collecting the drop of the first liquid of generation, the part of the outlet of described droplet treatment device does not contact with second liquid.First liquid does not contact with second liquid with changing in drop before changing into drop.
9. dropproducing devices according to claim 1, is characterized in that,
Described acceleration generation device is centrifuge.
10. dropproducing devices according to claim 1, is characterized in that,
Described acceleration can be constant or change.
11. dropproducing devices according to claim 1, is characterized in that,
Described microchannel is straight or bending.
12. dropproducing devices according to claim 1, is characterized in that,
Droplet treatment device is tube or the cone that there is opening front end.
13. dropproducing devices according to claim 1, is characterized in that,
Further comprise delay medium or deferred mount.
14. drop forming methods according to claim 1, is characterized in that,
Droplet treatment device and storage device are an entirety.
15. dropproducing devices according to claim 1, is characterized in that,
Droplet treatment device materials is inorganic material, organic material or composite.Inorganic material is glass, quartz, pottery, the one in metal.Organic material is polystyrene, polyether-ether-ketone, the one in polyester macromolecule material.Composite is the mixture of organic material described in one or more and/or inorganic material.
16. dropproducing devices according to claim 1, is characterized in that,
Described droplet treatment device whole or only exit portion, through hydrophobic and/or Hydrophilic Surface Treatment, make first liquid be hydrophobic state on the surface of droplet treatment device exit portion; Surface-treated method has: directly cover painting, and chemical reaction is modified, chemical deposition etc.
The production method of 17. 1 kinds of drops, is characterized in that, comprise acceleration generation device, it is for generation of centripetal acceleration; First liquid; Droplet treatment device, it possesses microchannel, and first liquid produces the drop of 5-300 μm of diameter under the effect of acceleration generation device by the microchannel of droplet treatment device; Gathering-device, it is for collecting the drop of generation; Wherein, first liquid is hydrophobic state on the surface of droplet treatment device exit portion.The internal diameter of described microchannel is 1-100 μm, and length is 0.1-10mm.
18. drop forming methods according to claim 17, is characterized in that,
First liquid is the solution of the materials such as water, cell culture fluid, ethanol, or comprises the combination of cell, DNA, RNA or protein in liquid.
19. drop forming methods according to claim 17, is characterized in that,
Droplet treatment device is the device of band microchannel, is selected from capillary, narrow meshed film or narrow meshed plate; The internal diameter of capillary or the diameter of aperture are 1-100 μm; The material of droplet treatment device can be inorganic material as glass, quartz, silicon; Or common organic plastic material is as polystyrene, Merlon, polymethylacrylic acid, polyamide, polyethylene, polyvinyl chloride, polypropylene; Fluoro-containing plastic as polytetrafluoroethylene (PTFE), PVDF etc.
20. drop forming methods according to claim 19, is characterized in that,
Described aperture is circular hole or polygonal hole; Internal diameter i.e. its length of side of described polygonal hole is 1-100 μm.
21. drop forming methods according to claim 17, is characterized in that,
The diameter of described drop is 10-250 μm, preferred 20-200 micron; The internal diameter of described microchannel is 2-90 micron, preferred 5-50 micron; The length of microchannel is 0.5-6mm, preferred 1-5mm.
22. drop forming methods according to claim 17, is characterized in that,
Droplet treatment device is 1,2 ... ..n, wherein n be greater than 2 integer.
23. drops according to claim 17 produce dress method, it is characterized in that,
Gathering-device is collected by centrifugation pipe.
24. drop forming methods according to claim 17, is characterized in that,
Further comprise the storage device of first liquid, it is for storing first liquid.
Further comprise second liquid, be positioned at gathering-device, for collecting the drop of the first liquid of generation, the part of the outlet of described droplet treatment device does not contact with second liquid.First liquid does not contact with second liquid with changing in drop before changing into drop.
25. drop forming methods according to claim 17, is characterized in that,
Described acceleration generation device is centrifuge.
26. drop forming methods according to claim 17, is characterized in that,
Described acceleration can be constant or change.
27. drop forming methods according to claim 17, is characterized in that,
Described microchannel is straight or bending.
28. drop forming methods according to claim 17, is characterized in that,
Droplet treatment device is tube or the cone that there is opening front end.
29. drop forming methods according to claim 17, is characterized in that,
Further comprise delay medium or deferred mount.
30. drop forming methods according to claim 24, is characterized in that,
Droplet treatment device and storage device are an entirety.
31. drop forming methods according to claim 17, is characterized in that,
Droplet treatment device materials is inorganic material, organic material or composite.Inorganic material is glass, quartz, pottery, the one in metal.Organic material is polystyrene, polyether-ether-ketone, the one in polyester macromolecule material.Composite is the mixture of organic material described in one or more and/or inorganic material.
32. drop forming methods according to claim 17, is characterized in that,
Described droplet treatment device whole or only exit portion, through hydrophobic and/or Hydrophilic Surface Treatment, make first liquid be hydrophobic state on the surface of droplet treatment device exit portion; Surface-treated method has: directly cover painting, and chemical reaction is modified, chemical deposition etc.
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