CN104737918A - Tissue culture fast breeding method of kiwi fruit rootstock M9 - Google Patents
Tissue culture fast breeding method of kiwi fruit rootstock M9 Download PDFInfo
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- CN104737918A CN104737918A CN201510199343.XA CN201510199343A CN104737918A CN 104737918 A CN104737918 A CN 104737918A CN 201510199343 A CN201510199343 A CN 201510199343A CN 104737918 A CN104737918 A CN 104737918A
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Abstract
The invention discloses a tissue culture fast breeding method of kiwi fruit rootstock M9. The tissue culture fast breeding method of the kiwi fruit rootstock M9 is characterized by comprising the following steps of (1) acquiring a non-bacteria material; (2) initially culturing kiwi fruits; (3) enrichment culturing the kiwi fruits; (4) rooting and hardening-off culturing; (5) finishing kiwi fruit rootstock fast breeding through an acclimatizating and transplanting step. The tissue culture fast breeding method of the kiwi fruit rootstock M9 has the beneficial effects that the method is adopted on the basis of an M9 culture medium, the types and the concentrations of major elements are adjusted, and hormone is optimized to adjust and control the agar concentration and the like, so that the balanced and reasonable nutritional requirement is provided for tissue culture seedling subculture multiplication of the kiwi fruits, fast, high-efficient and vigorous growth is ensured, the internode elongation is effectively promoted, and the growth coefficient is improved.
Description
Technical field
The present invention relates to the group training fast seedling-cultivating method of a kind of kiwi fruit stock M9.
Background technology
Kiwi fruit is the appellation of Chinese gooseberry cultivated species fruit.Also fox peach, Chinese gooseberry, carambola, wood, Mao Muguo, Chinese grooseberry, numb rattan fruit etc. are claimed.Originate in Hubei China Yichang City Yiling District mist to cross a river town, be distributed widely between southern china hills, the mountain ridge.Generally oval.Dark brown epidermis with hair generally do not eat, and are then the seed of pulp in bright green and row's black in it.The quality of kiwi fruit is soft, and taste is described to the mixing of strawberry, banana, pineapple three sometimes.Because of macaque eating, therefore named kiwi fruit; Also saying is had to be because pericarp covers hair, macaque and gaining the name seemingly.
At present, the technical problem of Kiwifruit Tissue Culture factorial seedling growth does not also solve completely, when adopting M9 medium Kiwifruit Tissue Culture seedling shoot proliferation to cultivate, often occur following problem: group training seedling base portion callus of taking root expands, plantlet in vitro growth potential is weak, blade is large, stem is short and small, internode elongation growth is slow, growth coefficient is low.
Summary of the invention
The object of this invention is to provide the group training fast seedling-cultivating method of a kind of kiwi fruit stock M9, solve the problem that kind of the Kiwifruit Tissue Culture stage improves growth coefficient, nurture body Optimal Growing.
The technical solution adopted for the present invention to solve the technical problems is:
A group training fast seedling-cultivating method of kiwi fruit stock M9, is characterized in that, comprise the following steps:
(1) sterilizable material is obtained:
The budlet that clip kiwi fruit is newly sprouted spring, after the alcohol immersion 20-30s cleaning material of 75%, is put in superclean bench on after soaking 15-20 minute in pure water, then inhales budlet soma surface moisture with blotting paper;
(2) Initial culture of kiwi fruit:
Get budlet base portion, be cut into the segment that lcm is long, be seeded on budlet Fiber differentiation, the composition of described budlet inducing culture is: M9+6-BA1.0mg/L+NAA0.2mg/L+ agar 8g/L+ sucrose 30g/L, budlet was inoculated in budlet inducing culture after 25 days, carried out shoot proliferation cultivation;
(3) Multiplying culture of kiwi fruit:
Bud with callus is inoculated on the adventitious bud proliferation medium that pH value is 5.8-6.2 and carries out Multiplying culture, the composition of described proliferated culture medium is by potassium nitrate 0.3mg/L+ ammonium nitrate 0.1mg/L+ potassium dihydrogen phosphate 0.1mg/L+ magnesium sulfate 0.2mg/L+ potassium iodide 0.2mg/L+ manganese sulphate 0.2mg/L+ zinc sulphate 0.3mg/L+ sodium molybdate 0.1mg/L+ ferrous sulfate 0.5mg/L+ thiamine hydrochloride 0.1mg/L+ puridoxine hydrochloride 0.2mg/L+ nicotinic acid 0.1mg/L+ heteroauxin 0.2mg/L+6-BA1.0-3.0mg/L+ sucrose 30g/L+ agar 6g/L, wherein, first carry out dark treatment 1 week, give illumination every day 16h again, the cultivation temperature of Multiplying culture is 24-26 DEG C,
(4) Rooting and hardening-off culture:
Induce Multiple Buds in adventitious bud proliferation medium after, Multiple Buds being divided into little Cong or simple bud and being inoculated in pH value is in the Rooting and hardening-off culture base of 5.8-6.2, the composition of described Rooting and hardening-off culture base is M9+NAA0.5-1.0mg/L+IBA2.0-6.0mg/L+ sucrose 30g/L+ agar 6g/L, carries out taking root and strong seedling culture;
(5) rooting culture:
When root length is for 2-4cm, carry out rooting culture, blake bottle is moved to hardening 6-10 days in booth, wash away medium, root is soaked 5% liquor potassic permanganate and carry out disinfection, be transplanted in the dish of cave, cover film, removes film after shading 55%, 1 week simultaneously, give full exposure, carry out rich water quality management.
Beneficial effect of the present invention: owing to adopting on the basis of M9 medium, by adjusting kind and the concentration of macroelement and optimizing hormone regulating and controlling agar concentration etc., for Kiwifruit Tissue Culture seedling shoot proliferation provides the nutritional need of balanced and reasonable, ensure that it is quick, efficient, robust growth, effectively facilitate internode elongation, improve growth coefficient.
Embodiment
embodiment:
A group training fast seedling-cultivating method of kiwi fruit stock M9, is characterized in that, comprise the following steps:
(1) sterilizable material is obtained:
The budlet that clip kiwi fruit is newly sprouted spring, after the alcohol immersion 20-30s cleaning material of 75%, soaks and is placed on superclean bench for 15-20 minute in pure water, then inhales budlet soma surface moisture with blotting paper;
(2) Initial culture of kiwi fruit:
Get budlet base portion, be cut into the segment that lcm is long, be seeded on budlet Fiber differentiation, the composition of described budlet inducing culture is: M9+6-BA1.0mg/L+NAA0.2mg/L+ agar 8g/L+ sucrose 30g/L, budlet was inoculated in budlet inducing culture after 25 days, carried out shoot proliferation cultivation;
(3) Multiplying culture of kiwi fruit:
Bud with callus is inoculated on the adventitious bud proliferation medium that pH value is 5.8-6.2 and carries out Multiplying culture, the composition of described proliferated culture medium is potassium nitrate 0.3mg/L+ ammonium nitrate 0.1mg/L+ potassium dihydrogen phosphate 0.1mg/L+ magnesium sulfate 0.2mg/L+ potassium iodide 0.2mg/L+ manganese sulphate 0.2mg/L+ zinc sulphate 0.3mg/L+ sodium molybdate 0.1mg/L+ ferrous sulfate 0.5mg/L+ thiamine hydrochloride 0.1mg/L+ puridoxine hydrochloride 0.2mg/L+ nicotinic acid 0.1mg/L+ heteroauxin 0.2mg/L+6-BA1.0-3.0mg/L+ sucrose 30g/L+ agar 6g/L, wherein, first carry out dark treatment 1 week, give illumination every day 16h again, the cultivation temperature of Multiplying culture is 24-26 DEG C,
(4) Rooting and hardening-off culture:
Induce Multiple Buds in adventitious bud proliferation medium after, Multiple Buds being divided into little Cong or simple bud and being inoculated in pH value is in the Rooting and hardening-off culture base of 5.8-6.2, the composition of described Rooting and hardening-off culture base is M9+NAA0.5-1.0mg/L+IBA2.0-6.0mg/L+ sucrose 30g/L+ agar 6g/L, carries out taking root and strong seedling culture;
(5) rooting culture:
When root length is for 2-4cm, carries out rooting culture, blake bottle is moved to hardening 6-10 days in booth, wash away medium, root soaks 5% liquor potassic permanganate and carries out disinfection, and be transplanted in the dish of cave, cover film, shades 55% simultaneously, remove film after 1 week, give full exposure, carry out rich water quality management.
Owing to adopting on the basis of M9 medium, by adjusting kind and concentration and the optimization hormone regulating and controlling agar concentration etc. of macroelement, for Kiwifruit Tissue Culture seedling shoot proliferation provides the nutritional need of balanced and reasonable, ensure that it is quick, efficient, robust growth, effectively facilitate internode elongation, improve growth coefficient.
Above embodiment is only be described the preferred embodiment of the present invention; not scope of the present invention is limited; under not departing from the present invention and designing the prerequisite of spirit; the various distortion that the common engineers and technicians in this area make technical scheme of the present invention and improvement, all should fall in protection domain that claims of the present invention determine.
The part that the present invention does not relate to prior art that maybe can adopt all same as the prior art is realized.
Claims (1)
1. a group training fast seedling-cultivating method of kiwi fruit stock M9, is characterized in that, comprise the following steps:
(1) sterilizable material is obtained:
The budlet that clip kiwi fruit is newly sprouted spring, after the alcohol immersion 20-30s cleaning material of 75%, is put in superclean bench on after soaking 15-20 minute in pure water, then inhales budlet soma surface moisture with blotting paper;
(2) Initial culture of kiwi fruit:
Get budlet base portion, be cut into the segment that lcm is long, be seeded on budlet Fiber differentiation, the composition of described budlet inducing culture is: M9+6-BA1.0mg/L+NAA0.2mg/L+ agar 8g/L+ sucrose 30g/L, budlet was inoculated in budlet inducing culture after 25 days, carried out shoot proliferation cultivation;
(3) Multiplying culture of kiwi fruit:
Bud with callus is inoculated on the adventitious bud proliferation medium that pH value is 5.8-6.2 and carries out Multiplying culture, the composition of described proliferated culture medium is by potassium nitrate 0.3mg/L+ ammonium nitrate 0.1mg/L+ potassium dihydrogen phosphate 0.1mg/L+ magnesium sulfate 0.2mg/L+ potassium iodide 0.2mg/L+ manganese sulphate 0.2mg/L+ zinc sulphate 0.3mg/L+ sodium molybdate 0.1mg/L+ ferrous sulfate 0.5mg/L+ thiamine hydrochloride 0.1mg/L+ puridoxine hydrochloride 0.2mg/L+ nicotinic acid 0.1mg/L+ heteroauxin 0.2mg/L+6-BA1.0-3.0mg/L+ sucrose 30g/L+ agar 6g/L, wherein, first carry out dark treatment 1 week, give illumination every day 16h again, the cultivation temperature of Multiplying culture is 24-26 DEG C,
(4) Rooting and hardening-off culture:
Induce Multiple Buds in adventitious bud proliferation medium after, Multiple Buds being divided into little Cong or simple bud and being inoculated in pH value is in the Rooting and hardening-off culture base of 5.8-6.2, the composition of described Rooting and hardening-off culture base is M9+NAA0.5-1.0mg/L+IBA2.0-6.0mg/L+ sucrose 30g/L+ agar 6g/L, carries out taking root and strong seedling culture;
(5) rooting culture:
When root length is for 2-4cm, carry out rooting culture, blake bottle is moved to hardening 6-10 days in booth, wash away medium, root is soaked 5% liquor potassic permanganate and carry out disinfection, be transplanted in the dish of cave, cover film, removes film after shading 55%, 1 week simultaneously, give full exposure, carry out rich water quality management.
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| CN201510199343.XA CN104737918A (en) | 2015-04-24 | 2015-04-24 | Tissue culture fast breeding method of kiwi fruit rootstock M9 |
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| CN201510199343.XA CN104737918A (en) | 2015-04-24 | 2015-04-24 | Tissue culture fast breeding method of kiwi fruit rootstock M9 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107278904A (en) * | 2017-08-07 | 2017-10-24 | 中国农业科学院郑州果树研究所 | A kind of Chinese Gooseberry cultural method and its application |
| CN107996410A (en) * | 2018-01-29 | 2018-05-08 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using Kiwi berry blade |
| CN108040883A (en) * | 2018-01-19 | 2018-05-18 | 陕西佰瑞猕猴桃研究院有限公司 | A kind of quick mating system of tissue cultures |
| CN109348953A (en) * | 2018-10-18 | 2019-02-19 | 南阳师范学院 | A kind of Kiwi berry culture of rootage and acclimatization and transplants method |
| CN111758573A (en) * | 2020-07-31 | 2020-10-13 | 武汉市农业科学院 | Tissue culture and rapid propagation method for delicious kiwi fruit rootstocks |
-
2015
- 2015-04-24 CN CN201510199343.XA patent/CN104737918A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 于红梅等: "海沃德猕猴桃带芽茎段的组织培养快繁技术", 《江苏农业科学》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107278904A (en) * | 2017-08-07 | 2017-10-24 | 中国农业科学院郑州果树研究所 | A kind of Chinese Gooseberry cultural method and its application |
| CN108040883A (en) * | 2018-01-19 | 2018-05-18 | 陕西佰瑞猕猴桃研究院有限公司 | A kind of quick mating system of tissue cultures |
| CN107996410A (en) * | 2018-01-29 | 2018-05-08 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using Kiwi berry blade |
| CN109348953A (en) * | 2018-10-18 | 2019-02-19 | 南阳师范学院 | A kind of Kiwi berry culture of rootage and acclimatization and transplants method |
| CN111758573A (en) * | 2020-07-31 | 2020-10-13 | 武汉市农业科学院 | Tissue culture and rapid propagation method for delicious kiwi fruit rootstocks |
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Application publication date: 20150701 |