CN1047318C

CN1047318C - Method for preparation of wound surface overlaying material

Info

Publication number: CN1047318C
Application number: CN92110212A
Authority: CN
Inventors: 刘喜生
Original assignee: Individual
Current assignee: Individual
Priority date: 1992-09-04
Filing date: 1992-09-04
Publication date: 1999-12-15
Anticipated expiration: 2007-09-04
Also published as: CN1083394A

Abstract

The present invention belongs to a preparation method for medical covering, particularly to the method that is particularly to suitable for preparing burn wound covering. Peritonea of animals, such as pigs, cattle, horses, etc. are mainly irradiated by gamma rays by the method, and necessary disinfecting treatment and germicidal treatment are performed before and after the irradiation. Thus, a prepared dry burn wound covering membrane and a prepared wet burn wound covering membrane have lower antigenicity, have comparatively ideal permeability and tightness, and can be widely used for new burn wounds, wound surfaces of granulation skin grafting and escharectomy wound surfaces. Wound surface infection can be effectively avoided, and the survival rate of skin grafting can also be increased.

Description

A kind of preparation method of wound-surface cover

The invention belongs to medical obducent preparation method, is a kind of obducent method of burn wound that is specially adapted to produce.

At present, Chang Yong burn wound's processing method roughly is divided into Drug therapy and in the biological epithelium two big classes that stick on the wound surface after chemistry and physical method processing.Drug therapy wherein is so-called dry exposed therapy and wet treatment.Dry exposure method is easily deepened burn wound in clinical practice.Wet treatment can be fit to bacterial growth because of the wound surface humidity, and easy giving birth to is infected, even can cause septicemia owing to adopting various Chinese herbal medicine unguentum and various cream, oil preparation coating wound surface.

Cover burn wound with biofilm, cadaver skin, Corii Sus domestica, artificial skin and people, cattle, the intravital biological abdomen of sheep of human body etc. are arranged.The covering that uses the human body skin to do to cut the crust wound surface is comparatively desirable, but produces rejection and its survival rate of raising for avoiding, and is higher to its clinical requirement, as must keep fresh etc. from the body bark fetching.Exist the shortage of skin source, (3000-5000 unit/rice costs an arm and a leg ²) and because chemosterilization does not thoroughly influence survival rate, and stick deficiencies such as preceding wound surface intractability is bigger.

Other epitheliums after physics and chemical method processing, as Corii Sus domestica, penta 2 Corii Sus domesticas, lyophilizing Corii Sus domestica, radiation Corii Sus domestica, pig amniotic membrane and artificial skin etc. also existing in various degree all, because its antigenicity is strong, easily take place heterogenous allosome is produced rejection, narrower with the scope of application, defectives such as the preparation difficulty is big, and the infection performance is relatively poor.

The object of the present invention is to provide the obducent method of a kind of preparation burn wound, this method is based on gamma-ray irradiation, and before and after the radiation to the animal peritoneum sterilization treatment that carries out disinfection, make the obducent antigenicity of made burn wound lower, the infection performance is higher, and have comparatively ideal permeability, plasticity and adhesion, an extension.To overcome the existing in prior technology deficiency.

The method that the animal peritoneum is prepared into burn wound's covering (being called for short the AP film) is:

Get Artiodactyla, Ruminantia, the mammiferous peritoneum of Perissodactyla and carry out basification, and behind the quinolones solution or liquor hibitane chemosterilization with valid density, pack in the aseptic nontoxic sealed container, be filled with an amount of nitrogen in the container, with static method the peritoneum in the container is carried out gamma-rays width of cloth photograph again, the irradiation total amount is 20 * 10 ³-40 * 10 ³GY makes the dried peritoneum goods that can preserve at normal temperatures; If after deflating in the sealed container, charge into an amount of nitrogen and aseptic blood plasma, the gamma-ray irradiation of reuse same amount is handled, and then makes the wet film goods.

Peritoneum choose and radiation before and after the sterilization processing method also have:

The animal peritoneum of choosing is the peritoneum of pig, cattle, horse, mule, donkey, and it is the surfactant solution of the representative sterilization treatment that carries out disinfection that predose also can adopt the 0.3-1% bromo geramine; Made dry film goods are contained antifungal drug solution with putting into after its aseptic taking-up before use, and soak at room temperature 20-50 minute stand-by.

The preparation irradiation of medicinal liquid is:

The used sterilization drug solution of predose is the bromo geramine of 0.3-1%, or the norfloxacin silver medicinal liquid that is equipped with norfloxacin and the silvery of 0.3-1%, also can select the silver sulfadiazine of 1-10% for use, or the peracetic acid liquid of 0.01-0.05%, gamma-ray irradiation can carry out at twice; As take irradiation one time, its irradiation is 25 * 10 ³-30 * 10 ³GY.

The antifungal liquid drug that soaks the dry film goods is to be mixed by the hibitane of 0.03-0.1% and 0.1-1% nystatin to make.

Below specific embodiments of the invention are described respectively:

1, the fresh peritoneum of getting pig, cattle, horse carries out basification earlier, with the hibitane medicinal liquid of 0.7% bromo geramine or 0.06% it is done sterilization then and handles.According to the needs of clinical use, the peritoneum after will handling under aseptic or half aseptic condition is made the covering goods of different size.If use nontoxic, aseptic plastic bag splendid attire, charge into an amount of nitrogen after the sealing again, and carry out gamma-ray irradiation with static method, irradiation is 30 * 10 ³GY after sampling Detection is aseptic, promptly becomes the obducent dry film goods of burn wound, can preserve at normal temperatures.This dry film goods must take out it under sterilising conditions in use, put into the medicinal liquid that is mixed and is made by 0.6% nystatin and 0.05% hibitane again, and room temperature (30 ℃) soaks the thing that sticks that was used for burn wound in 40 minutes, is the AP film.

2, to fresh animal peritoneums such as the pig that carried out basification, cattle, horses, the norfloxacin silver medicinal liquid that also can select for use norfloxacin and silvery by 0.3-1% to be equipped with is handled the sterilization that it carries out predose, irradiation such as above-mentioned, the used in use soak of the dry film goods behind the irradiation are 1% nystatin medicinal liquid.

The alkalization sterilization of predose is handled and is shaped as hereinbefore when 3, preparing wet film, sterilizing-and-disinfecting liquid also can consider to use the silver sulfadiazine of 1-10% or the peracetic acid liquid of 0.01-0.05%, in bulk diaphragm after the drug treating packed into fill in the glass container of aseptic blood plasma, deflate after the sealing, add an amount of nitrogen and carry out gamma-ray irradiation with static method then, irradiation is 25 * 10 ³GY, after sampling observation mensuration was aseptic, long preservation can be taken out at any time for clinical use at normal temperatures.

The substantive distinguishing features that the present invention has and obtained technological progress are:

1, the existing certain permeability of AP film through chemical sterilization and radiation treatment has again Seal preferably not only is conducive to the drainage of wound exudate but also can blocking diameter is 1 Passing through of coccus about μ m. Effectively solved the problem that burn wound easily infects, Also obviously reduced losing of surface of a wound moisture content and protein.

2, utilize the prepared AP film of method of the present invention not have obvious antigenicity, facing Bed can be avoided such as coverings such as cadaver skin, pigskins in using, and sticks to produce behind the surface of a wound High fever reaction can't dissolve the exposure surface of a wound too early. Its adhesion, the property upheld and plasticity All be better than other burn wound coverings.

3, use the AP film to cover the survival rate that the Micrograiting surface of a wound can improve Micrograiting, Make it does not cut the impact that the relatively poor or skin grafted wounds of scab surface of a wound condition has the factors such as oozing of blood.

4, the AP film is compared with other burn wound coverings in preparation, preserves and use, and the aspects such as performance have cheap (250 yuan/meter²), easy and simple to handle and be easy to the palm Hold, be convenient to the distinguishing features such as management. Can be widely used in the fresh surface of a wound of burning, the granulation skin-grafting The surface of a wound and cut the scab surface of a wound.

Claims

1. the preparation method of a wound-surface cover is that peritoneum with human body or animal carries out physics or chemical disinfection sterilization treatment, it is characterized in that getting Artiodactyla, Ruminantia, the mammiferous peritoneum of Perissodactyla and carry out basification, and behind the quinolones solution or liquor hibitane chemosterilization with valid density, pack in the aseptic nontoxic sealed container, be filled with an amount of nitrogen in the container, with static method the peritoneum in the container is carried out gamma-ray irradiation again, the irradiation total amount is 20 * 10 ³-40 * 10 ³GY makes the dried peritoneum goods that can preserve at normal temperatures.

2. the preparation method of a wound-surface cover is that peritoneum with human body or animal carries out physics or chemical disinfection sterilization treatment, it is characterized in that getting Artiodactyla, Ruminantia, the mammiferous peritoneum of Perissodactyla and carry out basification, and behind the quinolones solution or liquor hibitane chemosterilization with valid density, pack in the aseptic nontoxic sealed container, after the air extraction with sealed container, charge into an amount of nitrogen and aseptic blood plasma, with static method the peritoneum in the container is carried out gamma-ray irradiation again, the irradiation total amount is 20 * 10 ³～40 * 10 ³GY then is prepared into wet peritoneum goods.

3. obducent preparation method according to claim 1, it is characterized in that selected animal peritoneum is the peritoneum of pig, cattle, horse, mule, donkey, predose also can adopt the 0.3-1% bromo geramine sterilization treatment that carries out disinfection, to made dried peritoneum goods before use with after its aseptic taking-up, put into and contain antifungal drug solution, soak at room temperature 20-50 minute stand-by.

4. require 1 or 2 described obducent preparation methoies according to the power system, it is characterized in that the used sterilization drug solution of predose is the norfloxacin silver medicinal liquid that 0.3-1% bromo geramine or the norfloxacin of 0.3-1% and silvery are equipped with, also can select the peracetic acid liquid of 1-10% silver sulfadiazine or 0.01-0.05% for use, take irradiation one time, its irradiation is 25 * 10 ³-30 * 10 ³GY.