CN104721837A - Prevention and control method of porcine pseudorabies - Google Patents
Prevention and control method of porcine pseudorabies Download PDFInfo
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- CN104721837A CN104721837A CN201510091216.8A CN201510091216A CN104721837A CN 104721837 A CN104721837 A CN 104721837A CN 201510091216 A CN201510091216 A CN 201510091216A CN 104721837 A CN104721837 A CN 104721837A
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Abstract
The invention discloses a prevention and control method of porcine pseudorabies. Porcine pseudorabies gene-deleted vaccine immunity is carried out on all pigs in a pig breeding farm, then, purification treatment is carried out, breeding pigs with the positive wild virus infection test result are eliminated, then, remaining breeding pigs are divided into an antibody positive group and an antibody negative group, and then the antibody positive group is divided into a high antibody group and a low antibody group; vaccines are rebred into the antibody negative group and the low antibody group, then, purification treatment is carried out on breeding sows at a time every year, and purification treatment is carried out on breeding boars at a time every 6 months. The method is adopted to comprehensively prevent and control the porcine pseudorabies, the infection positive rate of the porcine pseudorabies is reduced year by year, through implementation of three years to four years, the virus detection result of the breeding boars and the breeding sows shows zero, and the pig farm obtains the obvious economic benefits and the social benefits. The method is simple and easy to implement, animals prone to infection are truly protected, the porcine pseudorabies of the pig farm can be completely and effectively purified and controlled, the PR purification effect is consolidated, and the prevention and control cost is greatly reduced.
Description
Technical field
The present invention relates to a kind of preventing control method of porcine pseudorabies, belong to Animal diseases immunological technique field.
Background technology
Porcine pseudorabies is by Pseudorabies virus
(Pseudorabiesvirus, PRV)what cause a kind ofly endangers serious acute infectious disease, mainly occurs nervous symptoms with the acute death of newborn piglet and piglet more than 4 week age, in-pig with breeding difficulty, miscarriage, stillborn fetus and respiratory symptom for feature.Porcine pseudorabies is widely current in China and falls ill heavier from the beginning of the nineties in last century, and there is the existence of this disease of report in district in all parts of the country.After the resistance to PRV actute infection excessively of pig at any age, all latent infection can be formed, virus can be hidden throughout one's life in pig body, under certain conditions, the virus of latency can be activated, cause recurrent infection and outside loose poison, this PRV circulative metabolism of hiding-activate determines PRV and forever exists in infection swinery, therefore causes high risks to pig industry.
The main mode of vaccine virus immunization that adopts carrys out anti-porcine pseudorabies processed at present, but vaccine immunity can only stop morbidity but can not stop existence and the activation of latent infection, causes huge obstacle thus to the purification of porcine pseudorabies and elimination.Along with gene-deleted vaccine exploitation and widely use, use and infect and immune antibody Differential Diagnosis technology, in the world a lot of national purification having carried out porcine pseudorabies, and obtain very large achievement.
But because the gene-deleted vaccine kind of present domestic use is many, missing gene is variant, research shows to use antibody differential diagnostic method be difficult to purification and control Pig Farm pseudorabies, and along with development in science and technology, the expense that experiment detects viral nucleic acid method reduces greatly.In addition when studying PRV purification, most researchers is due to the reason of time and funds, usually only 1 ~ 2 year is operated, only collect the means of production and the test material of pig farm a period of time, boar is not produced a cycle or adhere to performing and take into account at least 4 years, PR can not be consolidated and purify achievement, often cause PR prevention and control failure.
Summary of the invention
The problem that the harm caused swinery for current porcine pseudorabies is serious; the present invention proposes a kind of preventing control method of porcine pseudorabies; mainly for kind of a pig farm; use polymerase chain reaction method to detect the wild poison of porcine pseudorabies and determine positive swinery; open country poison purification is carried out to all boars, from the Sources controlling source of infection, detects immune antibody by head; certain protection susceptible animal, strengthens pig farm feeding and management and health care simultaneously.
The present invention is realized by following technical proposal: a kind of preventing control method of porcine pseudorabies, through following each step:
(1) Gene-deleted Vaccine of Swine Pseudorabies immunity is carried out to all pigs on kind of pig farm;
(2) vaccine immunity of step (1) starts after one month to carry out purified treatment, and namely to broad sow blood sampling, blood sampling comprises adopts anticoagulation and whole blood, to breeding boar semen collection liquid;
(3) by step (2) institute semen collection liquid and anticoagulation Conventional polymerase chain formula reaction do wild virus infection detect, step (2) is adopted in whole blood extract serum do immune antibody detection;
(4) boar that the result that step (3) wild virus infection detects is positive is eliminated, and colony house is carried out disinfection; Being divided into antibody positive group and negative antibody group by remaining the result that boar detects by step (3) immune antibody by head again, then by the result that antibody positive group detects according to immune antibody, being divided into high antibody population and low antibody population;
(5) vaccine is reseeded to the negative antibody group of step (4) and low antibody population, immune antibody detection is carried out again after 20 days, till antibody horizontal rising reaches more than effect level, be still below effect level to antibody horizontal after three immune antibodys detect, then this boar is superseded or isolated rearing;
(6) continuous boar and the sow using same provenance for 3 years, and wild virus infection detection is carried out to newly-increased pig, leave and take the person of being negative, and vaccine immunity is carried out to newly-increased pig; Process once by step (3) ~ (5) every year to broad sow afterwards, within every 6 months, process once by step (3) ~ (5) to breeding boar; Meanwhile, routine is taked to manage and protect by following requirements:
A, enforcement closed management, regular fly eradication, and every 3 months kill mouse once;
B, by suspicious, positive and occur that the sick pig of clinical symptoms isolates in time, then the colony house polluted by it of sterilizing, prevents cause of disease from spreading; All detect the broad sow having porcine pseudorabies as symptoms such as miscarriage, product stillborn fetus, product mummy tires, the person of being positive eliminates without exception;
C, reinforcement feeding and management, improve swinery resistance, pig farm performs the production model of all-in and all-out; Full group regularly throws something and feeds health care medicine for 3 months once; Piglet is being weaned, is turning group, vaccine immunity or transport front and back 2 days, all adds anti-stress medicine or improves immunity medicine in drinking-water or feedstuff; It is the oxytetracycline of 30% toward birth canal interior perfusion concentration that sows farrowing afterbirth drains rear, prevents secondary infection; Piglet in 7,14,21 days respectively injection concentration be the oxytetracycline of 20%;
D, the piglet to give over to kind carry out wild virus infection detection when 100 age in days.
Anticoagulation and each 5 milliliters of whole blood are adopted in the blood sampling of described step (2).
The semen collection liquid of described step (2) is semen collection liquid 5 milliliters.
The antibody positive group of described step (4) and negative antibody group are that the result detected according to step (3) immune antibody is hived off by detection kit criterion.
The high antibody population of described step (4) and low antibody population are that the result detected according to step (3) immune antibody arranges by head to low by antibody horizontal is high; the boar of antibody horizontal more than protection is as high antibody population, and the boar of antibody horizontal below protection is as low antibody population.
The vaccine immunity of described step (6) carries out Gene-deleted Vaccine of Swine Pseudorabies intramuscular injection immunity to the large pig in newly-increased pig, carries out pseudorabies disease vaccine Nasal immunization to piglet.
The enforcement closed management of described step a carries out disinfection to turnover personnel and vehicle and passageway, place.
Described sterilization uses peracetic acid, glutaraldehyde and Operand to hocket sterilization, sterilizes every day 1 time.
The health care medicine of described step c is that astragalus polysaccharides adds broad ectrum antibiotic.
The advantage that the present invention possesses and effect: adopt method of the present invention to carry out the Synthetical prevention of porcine pseudorabies, the positive rate that porcine pseudorabies can be made to infect declines year by year, implemented through 3 to 4 years, breeding boar can be made, broad sow is with malicious testing result to be shown as 0, make pig farm obtain significant economic benefit and social benefit.Method provided by the invention is simple, really protects susceptible animal, and the fully effective purification of energy and control Pig Farm pseudorabies, consolidated the purification achievement of PR, greatly reduced prevention and control cost.
Detailed description of the invention
Below by embodiment, the present invention will be further described.
Embodiment 1
(1) Gene-deleted Vaccine of Swine Pseudorabies immunity is carried out to all pigs on kind of pig farm;
(2) vaccine immunity of step (1) starts after one month to carry out purified treatment, and namely to broad sow blood sampling, blood sampling comprises adopts anticoagulation and each 5 milliliters of whole blood, to breeding boar semen collection liquid 5 milliliters;
(3) by step (2) institute semen collection liquid and anticoagulation Conventional polymerase chain formula reaction do wild virus infection detect, step (2) is adopted in whole blood extract serum do immune antibody detection;
(4) boar that the result that step (3) wild virus infection detects is positive is eliminated, and colony house is carried out disinfection; Being divided into antibody positive group and negative antibody group by remaining the result that boar detects by step (3) immune antibody by head again, then by the result that antibody positive group detects according to immune antibody, being divided into high antibody population and low antibody population; Described antibody positive group and negative antibody group are that the result detected according to step (3) immune antibody is hived off by detection kit criterion; High antibody population and low antibody population are that the result detected according to step (3) immune antibody arranges by head to low by antibody horizontal is high, and the boar of antibody horizontal more than protection is as high antibody population, and the boar of antibody horizontal below protection is as low antibody population;
(5) vaccine is reseeded to the negative antibody group of step (4) and low antibody population, immune antibody detection is carried out again after 20 days, till antibody horizontal rising reaches more than effect level, be still below effect level to antibody horizontal after three immune antibodys detect, then this boar is superseded or isolated rearing;
(6) continuous boar and the sow using same provenance for 3 years, and wild virus infection detection is carried out to newly-increased pig, leave and take the person of being negative, and Gene-deleted Vaccine of Swine Pseudorabies intramuscular injection immunity is carried out to the large pig in newly-increased pig, pseudorabies disease vaccine Nasal immunization is carried out to piglet; Process once by step (3) ~ (5) every year to broad sow afterwards, within every 6 months, process once by step (3) ~ (5) to breeding boar; Meanwhile, routine is taked to manage and protect by following requirements:
A, implement closed management, use peracetic acid, glutaraldehyde and Operand to hocket sterilization to turnover personnel and vehicle and passageway, place, sterilize every day 1 time, regular fly eradication, and every 3 months kill mouse once;
B, by suspicious, positive and occur that the sick pig of clinical symptoms isolates in time, then the colony house polluted by it of sterilizing, prevents cause of disease from spreading; All detect the broad sow having porcine pseudorabies as symptoms such as miscarriage, product stillborn fetus, product mummy tires, the person of being positive eliminates without exception;
C, reinforcement feeding and management, improve swinery resistance, pig farm performs the production model of all-in and all-out; Full group within 3 months, regularly throw something and feed astragalus polysaccharides add broad ectrum antibiotic health care medicine once; Piglet is being weaned, is turning group, vaccine immunity or transport front and back 2 days, all adds anti-stress medicine or improves immunity medicine in drinking-water or feedstuff; It is the oxytetracycline of 30% toward birth canal interior perfusion concentration that sows farrowing afterbirth drains rear, prevents secondary infection; Piglet in 7,14,21 days respectively injection concentration be the oxytetracycline of 20%;
D, the piglet to give over to kind carry out wild virus infection detection when 100 age in days.
The effect assessment of pig farm Synthetical prevention porcine pseudorabies:
1. production and technical indication
A, broad sow production target: the mortality of tire number, broad sow amount of livestock on hand, average the number of live birth (the piglet number of survival in 12 hours puerperal), average litter size (comprising live son, stillborn fetus, mummy tire), sow is produced in annual.
B, breeding boar production target: the mortality of breeding boar amount of livestock on hand, boar.
C, piglet production target: weaned piglet survival rate, average weight of weaning litter, average price of deed rate.
D, other production targets: annual boar replacement and obsoleteness rate etc.
2. porcine pseudorabies decontamination index
The qualified index of A, antibody: immune antibody protective rate 100%, 3 years postoperative infection Antibody positive rate are 0.
The wild poison of B, porcine pseudorabies detects index: breeding boar seminal fluid recall rate is 0, and suspicious pathological material of disease recall rate is 0.
2006 to 2014 to certain Pig Farm pseudorabies prevention and control of Yiliang County, Kunming, Yunnan Province:
The porcine pseudorabies purification scheme implementation formulated by embodiment 1 for 2008, carries out the wild poison purification of porcine pseudorabies to 61, pig farm boar, carries out porcine pseudorabies Synthetical prevention for 2009 first.Porcine pseudorabies infects positive rate and declines year by year, and implemented through 5 years, pig farm boar livestock on hand reaches 118, and 2014 2 times breeding boar, broad sow are with malicious testing result to be shown as 0, and pig farm achieves obvious economic benefit and social benefit, sees the following form:
Within 2010 ~ 2014 years, carry out porcine pseudorabies to cultivation company of Qujing City of Yunnan Province Zhanyi County to purify, achieve significant economic benefit and control effect:
The porcine pseudorabies purification scheme implementation formulated by embodiment 1, carries out the wild poison purification of porcine pseudorabies to 224, pig farm boar, carries out porcine pseudorabies Synthetical prevention for 2010 first.Porcine pseudorabies infects positive rate and declines year by year, and implemented through 4 years, 2014 2 times breeding boar, broad sow are with malicious testing result to be shown as 0, and pig farm achieves obvious economic benefit and social benefit, sees the following form:
Claims (9)
1. a preventing control method for porcine pseudorabies, is characterized in that through following each step:
(1) Gene-deleted Vaccine of Swine Pseudorabies immunity is carried out to all pigs on kind of pig farm;
(2) vaccine immunity of step (1) starts after one month to carry out purified treatment, and namely to broad sow blood sampling, blood sampling comprises adopts anticoagulation and whole blood, to breeding boar semen collection liquid;
(3) by step (2) institute semen collection liquid and anticoagulation Conventional polymerase chain formula reaction do wild virus infection detect, step (2) is adopted in whole blood extract serum do immune antibody detection;
(4) boar that the result that step (3) wild virus infection detects is positive is eliminated, and colony house is carried out disinfection; Being divided into antibody positive group and negative antibody group by remaining the result that boar detects by step (3) immune antibody by head again, then by the result that antibody positive group detects according to immune antibody, being divided into high antibody population and low antibody population;
(5) vaccine is reseeded to the negative antibody group of step (4) and low antibody population, immune antibody detection is carried out again after 20 days, till antibody horizontal rising reaches more than effect level, be still below effect level to antibody horizontal after three immune antibodys detect, then this boar is superseded or isolated rearing;
(6) continuous boar and the sow using same provenance for 3 years, and wild virus infection detection is carried out to newly-increased pig, leave and take the person of being negative, and vaccine immunity is carried out to newly-increased pig; Process once by step (3) ~ (5) every year to broad sow afterwards, within every 6 months, process once by step (3) ~ (5) to breeding boar; Meanwhile, routine is taked to manage and protect by following requirements:
A, enforcement closed management, regular fly eradication, and every 3 months kill mouse once;
B, by suspicious, positive and occur that the sick pig of clinical symptoms isolates in time, then the colony house polluted by it of sterilizing, prevents cause of disease from spreading; To there being the broad sow of pseudorabies disease symptoms all to detect, the person of being positive eliminates without exception;
C, reinforcement feeding and management, improve swinery resistance, pig farm performs the production model of all-in and all-out; Full group regularly throws something and feeds health care medicine for 3 months once; Piglet is being weaned, is turning group, vaccine immunity or transport front and back 2 days, all adds anti-stress medicine or improves immunity medicine in drinking-water or feedstuff; It is the oxytetracycline of 30% toward birth canal interior perfusion concentration that sows farrowing afterbirth drains rear, prevents secondary infection; Piglet in 7,14,21 days respectively injection concentration be the oxytetracycline of 20%;
D, the piglet to give over to kind carry out wild virus infection detection when 100 age in days.
2. preventing control method according to claim 1, is characterized in that: anticoagulation and each 5 milliliters of whole blood are adopted in the blood sampling of described step (2).
3. preventing control method according to claim 1, is characterized in that: the semen collection liquid of described step (2) is semen collection liquid 5 milliliters.
4. preventing control method according to claim 1, is characterized in that: the antibody positive group of described step (4) and negative antibody group are that the result detected according to step (3) immune antibody is hived off by detection kit criterion.
5. preventing control method according to claim 1; it is characterized in that: the high antibody population of described step (4) and low antibody population are that the result detected according to step (3) immune antibody arranges by head to low by antibody horizontal is high; the boar of antibody horizontal more than protection is as high antibody population, and the boar of antibody horizontal below protection is as low antibody population.
6. preventing control method according to claim 1, is characterized in that: the vaccine immunity of described step (6) carries out Gene-deleted Vaccine of Swine Pseudorabies intramuscular injection immunity to the large pig in newly-increased pig, carries out pseudorabies disease vaccine Nasal immunization to piglet.
7. preventing control method according to claim 1, is characterized in that: the enforcement closed management of described step a carries out disinfection to turnover personnel and vehicle and passageway, place.
8. preventing control method according to claim 7, is characterized in that: described sterilization uses peracetic acid, glutaraldehyde and Operand to hocket sterilization, sterilizes every day 1 time.
9. preventing control method according to claim 1, is characterized in that: the health care medicine of described step c is that astragalus polysaccharides adds broad ectrum antibiotic.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108553641A (en) * | 2018-05-15 | 2018-09-21 | 贵州都匀市黔昌畜牧发展有限责任公司 | A kind of pseudoabies immunization method |
| CN110218762A (en) * | 2019-06-19 | 2019-09-10 | 湖南新南方养殖服务有限公司 | The method whether detection pig farm mouse and its living environment carry African swine fever virus |
| CN110742018A (en) * | 2019-11-05 | 2020-02-04 | 广东省农业科学院动物卫生研究所 | PRV infected positive pig farm pseudorabies purification method |
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| CN104335973A (en) * | 2014-11-14 | 2015-02-11 | 上海费斯福生物科技有限公司 | Technology for improving survival rate and promoting quick growth of piglets |
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| CN102599103A (en) * | 2012-03-12 | 2012-07-25 | 云南农业大学 | Method for controlling porcine respiratory disease complex |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108553641A (en) * | 2018-05-15 | 2018-09-21 | 贵州都匀市黔昌畜牧发展有限责任公司 | A kind of pseudoabies immunization method |
| CN110218762A (en) * | 2019-06-19 | 2019-09-10 | 湖南新南方养殖服务有限公司 | The method whether detection pig farm mouse and its living environment carry African swine fever virus |
| CN110742018A (en) * | 2019-11-05 | 2020-02-04 | 广东省农业科学院动物卫生研究所 | PRV infected positive pig farm pseudorabies purification method |
| CN110742018B (en) * | 2019-11-05 | 2021-12-31 | 广东省农业科学院动物卫生研究所 | PRV infected positive pig farm pseudorabies purification method |
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