CN104713978A - Method for quantitatively detecting saponin components in Radix Ginseng Rubra and Radix Ophiopogonis injection - Google Patents
Method for quantitatively detecting saponin components in Radix Ginseng Rubra and Radix Ophiopogonis injection Download PDFInfo
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Abstract
The invention provides a method for quantitatively detecting saponin components in a Radix Ginseng Rubra and Radix Ophiopogonis injection. High performance liquid phase chromatography is adopted to simultaneously determine ginsenoside Rb1, Re and Rg1 and the content of at least one of the following saponin compounds: ginsenoside Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd and Rg3. The method comprises the following steps: preparing a mixed solution reference substance solution, preparing a tested substance solution, setting liquid phase determination conditions, and sampling the above sample, wherein the chromatographic conditions of liquid phase determination conditions comprise an octadecyl silane bonded silica gel column, acetonitrile-water as a mobile phase to carry out gradient elution and an ultraviolet detector for detection. The method has the characteristics of good specificity, few interference, high sensitivity, good precision, good reappearance, stable tested substance solution, accurate determination, fastness, accuracy and short analysis time.
Description
Technical field
The present invention relates to the method for quantitatively determining of saponin component in a kind of Shenmai injection, belong to Pharmaceutical Analysis field.
Background technology
Shenmai injection is by red ginseng and compound preparation that the tuber of dwarf lilyturf two, taste medicinal material extract was made, there is the function such as supplementing QI to prevent collapse, nourishing Yin and promoting production of body fluid, cure mainly the diseases such as vigour is lost, the few god of gas is tired, sweating Tianjin wound, asthma wish are de-, clear curative effect in angiocardiopathy and tumor aid treatment, and obtain clinical practice widely.Containing saponins, flavonoids, saccharide compound isoreactivity composition in Shenmai injection, wherein ginsenoside comprises protopanaxadiol-type's saponin(e (ginsenoside Rb1, Rb2, Rc, Rd), Protopanaxatriol's type saponin(e (ginsenoside Re, Rg1, Rf), the composition such as oleanolic acid type ginsenoside (ginsenoside Ro) and other (ginsenoside Rg2, Rh1, Rh2, Rg3, Rg5, Rg6, Rs4-Rs7, Rk1-Rk3), and ophiopogonin D is the representative saponin(e in the tuber of dwarf lilyturf.
Existing operative norm (the national drug standards WS of Shenmai injection
3-B-3428-98-2010) define the Qualitive test of ginsenoside Rb1, Rg1, Re, and total saposins and ginsenoside Rb1, Rg1, Re quantitative measurement, wherein adopt colorimetric method for determining total saposins, specificity is not strong.In view of complicated component in Shenmai injection and various component content difference is very large, and in existing Shenmai injection, the quantitative measurement of saponin component only measures a few ginsenoside, is difficult to control its quality comprehensively.Therefore, be badly in need of a kind of quantitative detecting method that once can measure more than ten kind of saponin component, to strengthen the quality control of Shenmai injection, improve validity and the controllability of product.
Summary of the invention
The invention provides the quantitative detecting method of saponin component in a kind of Shenmai injection, it comprises the following steps:
(a) mixing reference substance solution preparation: precision takes ginsenoside Rb1, Re, Rg1, the reference substance also comprising at least one in ginsenoside Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd or Rg3 is appropriate, adds 50% methyl alcohol and makes mixing reference substance solution;
B prepared by () need testing solution: it is appropriate that precision measures Shenmai injection, makes need testing solution;
C () liquid phase condition determination: octadecylsilane chemically bonded silica reverse-phase chromatographic column is that mobile phase carries out gradient elution with acetonitrile-water, UV-detector detects; Wherein, the volume ratio of described acetonitrile-water gradient is 18% → 22%:82% → 78% (0 ~ 27min), 22% → 26%:78% → 74% (27 ~ 28min), 26% → 31%:74% → 69% (28 ~ 65min), 31% → 39%:69% → 61% (65 ~ 80min), 39% → 50%:61% → 50% (80 ~ 105min), 50% → 60%:50% → 40% (105 ~ 115min), 60% → 75%:40% → 25% (115 ~ 125min), 75%:25% (125 ~ 135min);
(d) sample determination: get mixing reference substance solution and need testing solution each 20-30 μ L, injection liquid chromatography measures, each saponin component content in external standard method calculation sample.
Quantitative detecting method of the present invention, be applicable to ginsenoside Rb1 in Shenmai injection, Re, Rg1, also comprise the assay of the saponins compound of at least one in ginsenoside Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd or Rg3, or also comprise the assay of saponins compound of similar.Those skilled in the art can select suitable reference substance preparation mixing reference substance solution according to material to be detected.
In certain preferred embodiments of the present invention, mix reference substance solution preparation method described in step (a) and comprise that precision takes ginsenoside Rb1, Re, Rg1, Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd and Rg3 are appropriate, add 50% methyl alcohol and make mixing reference substance solution.
In certain preferred embodiments of the present invention, mix ginsenoside Rg1 in reference substance solution described in step (a), the concentration of Re, Rf, Rb1, Rc, Rb2, Rb3, Rd or Rg3 is 0.20mg/mL, the concentration of ginsenoside Rh 1 or Rg2 is 0.10mg/mL.
In certain preferred embodiments of the present invention, chromatographic condition described in step (c) is: flow velocity is 1mL/min; Column temperature is 30 DEG C; Sample size is 20 μ L; Determined wavelength is 203nm.
In certain preferred embodiments of the present invention, described in step (b), need testing solution is prepared as follows: precision measures Shenmai injection 5mL, crosses solid phase extraction column (Alltech 500mg × 4mL), uses 5mL water successively, 5mL 30% methyl alcohol, 5mL methanol-eluted fractions, collects methanol-eluted fractions, and is transferred in 5mL volumetric flask, add methanol constant volume to scale, shake up, 0.45 μm of miillpore filter filters, and to obtain final product.
Further, in a preferred embodiment of the invention, quantitative detecting method of the present invention comprises the steps:
(a) mixing reference substance solution preparation: mixing reference substance solution preparation: precision takes ginsenoside Rb1, Re, Rg1, the reference substance also comprising at least one in ginsenoside Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd or Rg3 is appropriate, add 50% methyl alcohol and make mixing reference substance solution, in wherein said mixing reference substance solution, the concentration of ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3, Rd or Rg3 is 0.20mg/mL, and the concentration of ginsenoside Rh 1 or Rg2 is 0.10mg/mL;
B prepared by () need testing solution: precision measures Shenmai injection 5mL, cross solid phase extraction column (Alltech 500mg × 4mL), use 5mL water successively, 5mL 30% methyl alcohol, 5mL methanol-eluted fractions, collect methanol-eluted fractions, and be transferred in 5mL volumetric flask, add methanol constant volume to scale, shake up, 0.45 μm of miillpore filter filters, and makes need testing solution;
(c) liquid phase condition determination: C
18analytical column (250mm × 4.6mm, 5 μm), be that mobile phase carries out gradient elution with acetonitrile-water, flow velocity is 1mL/min, column temperature is 30 DEG C, sample size is 20 μ L, determined wavelength is 203nm, wherein, the volume ratio of described acetonitrile-water gradient is 18% → 22%:82% → 78% (0 ~ 27min), 22% → 26%:78% → 74% (27 ~ 28min), 26% → 31%:74% → 69% (28 ~ 65min), 31% → 39%:69% → 61% (65 ~ 80min), 39% → 50%:61% → 50% (80 ~ 105min), 50% → 60%:50% → 40% (105 ~ 115min), 60% → 75%:40% → 25% (115 ~ 125min), 75%:25% (125 ~ 135min),
(d) sample determination: get mixing reference substance solution and need testing solution, injection liquid chromatography measures, each saponin component content in external standard method calculation sample.
Further, in a preferred embodiment of the invention, quantitative detecting method of the present invention comprises the steps:
(a) mixing reference substance solution preparation: precision take ginsenoside Rb1, Re, Rg1, Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd and Rg3 reference substance appropriate, add 50% methyl alcohol and make mixing reference substance solution, in wherein said mixing reference substance solution, the concentration of ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3, Rd or Rg3 is 0.20mg/mL, and the concentration of ginsenoside Rh 1 or Rg2 is 0.10mg/mL;
B prepared by () need testing solution: precision measures Shenmai injection 5mL, cross solid phase extraction column (Alltech 500mg × 4mL), use 5mL water successively, 5mL 30% methyl alcohol, 5mL methanol-eluted fractions, collect methanol-eluted fractions, and be transferred in 5mL volumetric flask, add methanol constant volume to scale, shake up, 0.45 μm of miillpore filter filters, and makes need testing solution;
(c) liquid phase condition determination: C
18analytical column (250mm × 4.6mm, 5 μm), be that mobile phase carries out gradient elution with acetonitrile-water, flow velocity is 1mL/min, column temperature is 30 DEG C, sample size is 20 μ L, determined wavelength is 203nm, wherein, the volume ratio of described acetonitrile-water gradient is 18% → 22%:82% → 78% (0 ~ 27min), 22% → 26%:78% → 74% (27 ~ 28min), 26% → 31%:74% → 69% (28 ~ 65min), 31% → 39%:69% → 61% (65 ~ 80min), 39% → 50%:61% → 50% (80 ~ 105min), 50% → 60%:50% → 40% (105 ~ 115min), 60% → 75%:40% → 25% (115 ~ 125min), 75%:25% (125 ~ 135min),
(d) sample determination: get mixing reference substance solution and need testing solution, injection liquid chromatography measures, each saponin component content in external standard method calculation sample.
The present invention can multiple saponin component in Simultaneously test Shenmai injection, it is applicable to the quality control of saponin component in Shenmai injection finished product and production run thereof, especially assay, sample size and peak area are good linear relationship within the specific limits for they, there is specificity good, disturb less, highly sensitive, precision is good, favorable reproducibility, need testing solution is stablized, method measures accurately, method is fast accurate, the features such as shorter are asked when analyzing relative, the Ginsenosides (as ginsenoside Rb1) higher to concentration and the lower Ginsenosides of concentration are (as ginsenoside Rb3, Rf) all can carry out accurate quantitative analysis feature simultaneously, and test sample disposal route is simple, detecting instrument is conventional is easy to operation, method is simple and efficient.
Accompanying drawing explanation
Fig. 1 Shenmai injection HPLC collection of illustrative plates
Fig. 2 ginsenoside reference substance HPLC schemes
Specific embodiments
By the following examples the present invention be further explained and illustrate, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following embodiment.Without departing from the idea case in the present invention described above, the various replacement made according to ordinary skill knowledge and customary means or change, all should comprise within the scope of the invention.
Embodiment 1
1, instrument and reagent
Agilent Series 1200 high performance liquid chromatograph, comprises automatic sampler, binary pump, column oven, online degasser and DAD detecting device.
Acetonitrile is Merck Reagent Company product; Methyl alcohol is pure for analyzing, traditional Chinese medicines Group Co., Ltd product; Water is Robust distilled water; Reference substance ginsenoside Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd, Rg3 are all purchased from Nat'l Pharmaceutical & Biological Products Control Institute; Shenmai injection is provided by Hebei Shineway Pharmaceutical Co., Ltd.
2, method and result
(1) chromatographic condition
Chromatographic column: C
18analytical column (250mm × 4.6mm, 5 μm); Mobile phase: be mobile phase A with acetonitrile, take water as Mobile phase B, according to the form below carries out gradient elution; Flow velocity: 1mL/min; Column temperature: 30 DEG C; UV-detector; Determined wavelength 203nm.
(3) reference substance solution preparation is mixed
Take each ginsenoside reference substance appropriate, accurately weighed, add 50% methyl alcohol and make every 1mL respectively containing the mixing reference substance solution of ginsenoside Rg1, the equal 0.20mg of Re, Rf, Rb1, Rc, Rb2, Rb3, Rd, Rg3 and ginsenoside Rh 1, the equal 0.10mg of Rg2, for subsequent use.
(4) need testing solution preparation
Precision measures Shenmai injection 5mL, cross solid phase extraction column (Alltech 500mg × 4mL), use 5mL water, 5mL30% methyl alcohol, 5mL methanol-eluted fractions successively, collect methanol-eluted fractions, and be transferred in 5mL volumetric flask, add methanol constant volume to scale, shake up, 0.45 μm of miillpore filter filters, and to obtain final product.
(5) negative control solution preparation
Removing red ginseng and the tuber of dwarf lilyturf medicinal material, make containing red ginseng and the sample of the tuber of dwarf lilyturf according to Shenmai injection production technology (see national standard WS3-B-3428-98-2010), make negative control solution according under need testing solution preparation.
(6) system suitability test
Accurate absorption mixes reference substance solution 20 μ L, injection liquid chromatography, repeats 6 times, measures each chromatogram peak-to-peak area, the relative standard deviation of the chromatographic peak peak area of calculating ginsenoside Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd and Rg3, the results are shown in Table 1.Result shows, this method system flexibility is good, instrument precision is better.
Table 1 system flexibility result
| Ginsenoside | 1 | 2 | 3 | 4 | 5 | 6 | Average peak area | RSD(％) |
| Rg1 | 662.2 | 650.4 | 664.7 | 657.2 | 640.6 | 645.3 | 655.0 | 1.49 |
| Re | 362.6 | 352.3 | 368.4 | 354.7 | 370.1 | 360.2 | 361.4 | 2.20 |
| Rf | 414.6 | 410.8 | 416.7 | 409.2 | 420.3 | 405.7 | 412.9 | 1.08 |
| Rh1/Rg2 | 748.2 | 722.9 | 736.8 | 745.2 | 761.3 | 753.6 | 744.7 | 1.91 |
| Rb1 | 1540.2 | 1480.6 | 1509.3 | 1520.5 | 1583.2 | 1620.5 | 1542.4 | 2.48 |
| Rc | 635.5 | 647.8 | 650.2 | 660.7 | 645.9 | 662.3 | 650.4 | 1.39 |
| Rb2 | 614.6 | 608.9 | 635.3 | 622.7 | 618.9 | 627.4 | 621.3 | 1.60 |
| Rb3 | 212.7 | 214.5 | 205.3 | 210.7 | 218.3 | 220.9 | 213.7 | 2.25 |
| Rd | 295.2 | 300.2 | 304.2 | 298.4 | 287.5 | 293.4 | 296.5 | 2.12 |
| Rg3 | 149.5 | 153.2 | 148.9 | 145.6 | 150.2 | 147.6 | 149.2 | 1.83 |
(7) specificity test
Accurate absorption negative control solution, each 20 μ L of mixing reference substance solution respectively, injection liquid chromatography, record chromatogram.Result shows, negative control solution respectively goes out the existence at noiseless peak, relevant position, peak at mixing contrast solution, and the mensuration of component to ginsenoside Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd and Rg3 namely in Shenmai injection beyond red ginseng extract and ophiopogon japonicus extract is noiseless.
(8) linear relationship is investigated
Accurate absorption mixes reference substance solution 1 μ L, 5 μ L, 10 μ L, 20 μ L, 30 μ L respectively, injection liquid chromatography measures, respectively with each reference substance peak area (Y) for ordinate, with sample size (X, μ L) carry out linear regression for horizontal ordinate, try to achieve regression equation, the results are shown in Table 2.
Table 2 linear relationship result
| Ginsenoside | Linear equation | The range of linearity |
| Rg1 | y＝333.19x-11.2180，r 2＝0.9996 | 0.1868-5.6040(μg) |
| Re | y＝363.13x+0.9488，r 2＝0.9993 | 0.1854-5.5620(μg) |
| Rf | y＝416.33x+0.4784，r 2＝0.9995 | 0.1876-5.6280(μg) |
| Rh1/Rg2 | y＝373.51x+1.1305，r 2＝0.9997 | 0.1884-5.6520(μg) |
| Rb1 | y＝316.64x-10.3300，r 2＝0.9996 | 0.1858-5.5740(μg) |
| Rc | y＝321.26x-0.1262，r 2＝0.9999 | 0.1872-5.6160(μg) |
| Rb2 | y＝313.32x-11.1650，r 2＝0.9996 | 0.1856-5.5680(μg) |
| Rb3 | y＝434.61x-4.7722，r 2＝0.9993 | 0.1866-5.5980(μg) |
| Rd | y＝305.82x-3.9603，r 2＝0.9995 | 0.1874-5.6220(μg) |
| Rg3 | y＝149.88x+3.1045，r 2＝0.9997 | 0.1864-5.5920(μg) |
(9) precision is investigated
Get same batch of Shenmai injection (lot number 09101821) 6 parts respectively, need testing solution is made according under need testing solution preparation, the above-mentioned need testing solution of accurate absorption 20 μ L respectively, injection liquid chromatography measures, calculate content (μ g/mL), the results are shown in Table 3.Result shows, this method repeatability is good.
Table 3 precision investigates result
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | Average content | RSD(％) |
| Rg1 content | 88 | 86 | 89 | 87 | 90 | 88 | 88 | 1.61 |
| Re content | 43 | 41 | 43 | 44 | 43 | 42 | 43 | 2.42 |
| Rf content | 38 | 36 | 37 | 36 | 35 | 37 | 37 | 2.87 |
| Rh1/Rg2 content | 87 | 89 | 90 | 88 | 89 | 87 | 88 | 1.37 |
| Rb1 content | 283 | 288 | 280 | 282 | 278 | 285 | 283 | 1.26 |
| Rc content | 122 | 123 | 126 | 120 | 125 | 119 | 123 | 2.24 |
| Rb2 content | 124 | 122 | 126 | 125 | 124 | 123 | 124 | 1.14 |
| Rb3 content | 16 | 15 | 16 | 16 | 16 | 15 | 16 | 3.30 |
| Rd content | 70 | 69 | 72 | 70 | 71 | 70 | 70 | 1.47 |
| Rg3 content | 73 | 73 | 72 | 70 | 71 | 72 | 72 | 1.63 |
(10) stability test
Get same batch of Shenmai injection (lot number 09101821), need testing solution is made according under need testing solution preparation, draw 20 μ L injection liquid chromatographies in 0h, 2h, 4h, 8h, 12h, 24h precision respectively to measure, calculate content (μ g/mL), the results are shown in Table 4.Result shows, need testing solution is at least stable in 24h.
Table 4 stability test result
| Numbering | 0h | 2h | 4h | 8h | 12h | 24h | Average content | RSD(％) |
| Rg1 content | 89 | 88 | 87 | 88 | 87 | 87 | 88 | 0.93 |
| Re content | 44 | 43 | 43 | 44 | 43 | 42 | 43 | 1.74 |
| Rf content | 37 | 38 | 37 | 36 | 36 | 37 | 37 | 2.04 |
| Rh1/Rg2 content | 88 | 87 | 87 | 88 | 89 | 87 | 88 | 0.93 |
| Rb1 content | 286 | 285 | 288 | 286 | 284 | 285 | 286 | 0.48 |
| Rc content | 124 | 123 | 126 | 120 | 125 | 124 | 124 | 1.67 |
| Rb2 content | 122 | 124 | 126 | 125 | 124 | 123 | 124 | 1.14 |
| Rb3 content | 15 | 15 | 15 | 15 | 16 | 15 | 15 | 2.69 |
| Rd content | 69 | 70 | 71 | 70 | 71 | 70 | 70 | 1.07 |
| Rg3 content | 72 | 73 | 72 | 72 | 71 | 72 | 72 | 0.88 |
(11) recovery test
Get the injection containing ginseng extract liquor 9 parts with a collection of (lot number 09102121) known content respectively, precision measures 5mL, cross solid phase extraction column (Alltech 500mg × 4mL), use 5mL water successively, 5mL 30% methyl alcohol, 5mL methanol-eluted fractions, collect methanol-eluted fractions, and be transferred in 10mL volumetric flask, then precision adds mixing reference substance solution 2mL, 2.5mL and 3mL respectively, adds methanol constant volume to scale, shake up, accurate 20 μ L injection liquid chromatographies of drawing measure, and calculate average recovery, the results are shown in Table 5.Result shows, the recovery of this method is good, accuracy is high.
Table 5 average recovery test findings
3, sample determination
Get 10 batches of Shenmai injections and make need testing solution according under test sample preparation, accurate absorption mixes reference substance solution and each 20 μ L of need testing solution, and injection liquid chromatography measures, and calculates content (μ g/mL), the results are shown in Table 6.
The content of saponin component in table 6 Shenmai injection
| Sample number into spectrum | Rg1 | Re | Rf | Rh1/Rg2 | Rb1 | Rc | Rb2 | Rb3 | Rd | Rg3 |
| 09101821 | 88 | 44 | 37 | 87 | 286 | 121 | 124 | 16 | 69 | 72 |
| 09101921 | 87 | 47 | 35 | 83 | 279 | 118 | 120 | 16 | 65 | 64 |
| 09102021 | 82 | 45 | 37 | 91 | 293 | 123 | 124 | 17 | 69 | 74 |
| 09102121 | 80 | 43 | 38 | 79 | 281 | 119 | 121 | 15 | 65 | 58 |
| 09102221 | 81 | 44 | 38 | 86 | 280 | 117 | 119 | 15 | 65 | 63 |
| 09102321 | 86 | 43 | 38 | 73 | 278 | 118 | 119 | 15 | 65 | 52 |
| 09102421 | 83 | 45 | 37 | 91 | 292 | 123 | 125 | 16 | 69 | 73 |
| 09102521 | 87 | 43 | 34 | 73 | 258 | 107 | 110 | 15 | 61 | 69 |
| 09102621 | 88 | 45 | 36 | 80 | 286 | 123 | 124 | 16 | 68 | 67 |
| 09102721 | 81 | 54 | 36 | 70 | 272 | 115 | 121 | 15 | 63 | 52 |
The present invention has carried out accurate quantitative analysis to ginsenoside Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd and Rg3 11 kinds of saponins compounds in Shenmai injection first simultaneously.Wherein, due to ginsenoside Re and Rd, Ginsenoside Rc and Rb2 are respectively isomers, therefore adopt acetonitrile-water be eluent gradient wash-out respectively to ginsenoside Re and Rd, Ginsenoside Rc is separated with Rb2.Method of the present invention have specificity good, disturb less, highly sensitive, ask that shorter, higher to concentration Ginsenosides (as ginsenoside Rb1) and the lower Ginsenosides (as ginsenoside Rb3, Rf) of concentration all can carry out accurate quantitative analysis simultaneously when analyzing relative.
Claims (7)
1. the quantitative detecting method of saponin component in Shenmai injection, is characterized in that, comprise the following steps:
(a) mixing reference substance solution preparation: precision takes ginsenoside Rb1, Re, Rg1, the reference substance also comprising at least one in ginsenoside Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd or Rg3 is appropriate, adds 50% methyl alcohol and makes mixing reference substance solution;
B prepared by () need testing solution: it is appropriate that precision measures Shenmai injection, makes need testing solution;
C () liquid phase condition determination: octadecylsilane chemically bonded silica reverse-phase chromatographic column is that mobile phase carries out gradient elution with acetonitrile-water, UV-detector detects; Wherein, the volume ratio of described acetonitrile-water gradient is 18% → 22%:82% → 78% (0 ~ 27min), 22% → 26%:78% → 74% (27 ~ 28min), 26% → 31%:74% → 69% (28 ~ 65min), 31% → 39%:69% → 61% (65 ~ 80min), 39% → 50%:61% → 50% (80 ~ 105min), 50% → 60%:50% → 40% (105 ~ 115min), 60% → 75%:40% → 25% (115 ~ 125min), 75%:25% (125 ~ 135min);
(d) sample determination: get mixing reference substance solution and need testing solution each 20-30 μ L, injection liquid chromatography measures, each saponin component content in external standard method calculation sample.
2. quantitative detecting method according to claim 1, it is characterized in that, mix reference substance solution preparation method described in step (a) and comprise that precision takes ginsenoside Rb1, Re, Rg1, Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd and Rg3 are appropriate, add 50% methyl alcohol and make mixing reference substance solution.
3. quantitative detecting method according to claim 1 and 2, it is characterized in that, mix ginsenoside Rg1 in reference substance solution described in step (a), the concentration of Re, Rf, Rb1, Rc, Rb2, Rb3, Rd or Rg3 is 0.20mg/mL, the concentration of ginsenoside Rh 1 or Rg2 is 0.10mg/mL.
4. quantitative detecting method according to claim 1 and 2, is characterized in that, chromatographic condition described in step (c) is: flow velocity is 1mL/min; Column temperature is 30 DEG C; Sample size is 20 μ L; Determined wavelength is 203nm.
5. quantitative detecting method according to claim 1 and 2, is characterized in that, described in step (b), need testing solution is prepared as follows: precision measures Shenmai injection 5mL, cross solid phase extraction column (Alltech 500mg × 4mL), use 5mL water successively, 5mL 30% methyl alcohol, 5mL methanol-eluted fractions, collect methanol-eluted fractions, and be transferred in 5mL volumetric flask, add methanol constant volume to scale, shake up, 0.45 μm of miillpore filter filters, and to obtain final product.
6. the quantitative detecting method of saponin component in wheat parenteral solution, is characterized in that, comprise following:
(a) mixing reference substance solution preparation: mixing reference substance solution preparation: precision takes ginsenoside Rb1, Re, Rg1, the reference substance also comprising at least one in ginsenoside Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd or Rg3 is appropriate, add 50% methyl alcohol and make mixing reference substance solution, in wherein said mixing reference substance solution, the concentration of ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3, Rd or Rg3 is 0.20mg/mL, and the concentration of ginsenoside Rh 1 or Rg2 is 0.10mg/mL;
B prepared by () need testing solution: precision measures Shenmai injection 5mL, cross solid phase extraction column (Alltech 500mg × 4mL), use 5mL water successively, 5mL 30% methyl alcohol, 5mL methanol-eluted fractions, collect methanol-eluted fractions, and be transferred in 5mL volumetric flask, add methanol constant volume to scale, shake up, 0.45 μm of miillpore filter filters, and makes need testing solution;
(c) liquid phase condition determination: C
18analytical column (250mm × 4.6mm, 5 μm), be that mobile phase carries out gradient elution with acetonitrile-water, flow velocity is 1mL/min, column temperature is 30 DEG C, sample size is 20 μ L, determined wavelength is 203nm, wherein, the volume ratio of described acetonitrile-water gradient is 18% → 22%:82% → 78% (0 ~ 27min), 22% → 26%:78% → 74% (27 ~ 28min), 26% → 31%:74% → 69% (28 ~ 65min), 31% → 39%:69% → 61% (65 ~ 80min), 39% → 50%:61% → 50% (80 ~ 105min), 50% → 60%:50% → 40% (105 ~ 115min), 60% → 75%:40% → 25% (115 ~ 125min), 75%:25% (125 ~ 135min),
(d) sample determination: get mixing reference substance solution and need testing solution, injection liquid chromatography measures, each saponin component content in external standard method calculation sample.
7. the quantitative detecting method of saponin component in wheat parenteral solution, is characterized in that, comprise following:
(a) mixing reference substance solution preparation: precision take ginsenoside Rb1, Re, Rg1, Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd and Rg3 reference substance appropriate, add 50% methyl alcohol and make mixing reference substance solution, in wherein said mixing reference substance solution, the concentration of ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3, Rd or Rg3 is 0.20mg/mL, and the concentration of ginsenoside Rh 1 or Rg2 is 0.10mg/mL;
B prepared by () need testing solution: precision measures Shenmai injection 5mL, cross solid phase extraction column (Alltech 500mg × 4mL), use 5mL water successively, 5mL 30% methyl alcohol, 5mL methanol-eluted fractions, collect methanol-eluted fractions, and be transferred in 5mL volumetric flask, add methanol constant volume to scale, shake up, 0.45 μm of miillpore filter filters, and makes need testing solution;
(c) liquid phase condition determination: C
18analytical column (250mm × 4.6mm, 5 μm), be that mobile phase carries out gradient elution with acetonitrile-water, flow velocity is 1mL/min, column temperature is 30 DEG C, sample size is 20 μ L, determined wavelength is 203nm, wherein, the volume ratio of described acetonitrile-water gradient is 18% → 22%:82% → 78% (0 ~ 27min), 22% → 26%:78% → 74% (27 ~ 28min), 26% → 31%:74% → 69% (28 ~ 65min), 31% → 39%:69% → 61% (65 ~ 80min), 39% → 50%:61% → 50% (80 ~ 105min), 50% → 60%:50% → 40% (105 ~ 115min), 60% → 75%:40% → 25% (115 ~ 125min), 75%:25% (125 ~ 135min),
(d) sample determination: get mixing reference substance solution and need testing solution, injection liquid chromatography measures, each saponin component content in external standard method calculation sample.
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