CN104710016B - A kind of method going heavy metal in eliminating water using Properties of Mangrove Seedlings - Google Patents
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- 240000002044 Rhizophora apiculata Species 0.000 title claims abstract description 53
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 19
- 235000016709 nutrition Nutrition 0.000 claims abstract description 28
- 150000002500 ions Chemical class 0.000 claims abstract description 21
- 230000006872 improvement Effects 0.000 claims abstract description 10
- 239000001110 calcium chloride Substances 0.000 claims abstract description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 4
- 238000011534 incubation Methods 0.000 claims abstract description 4
- 239000011575 calcium Substances 0.000 claims description 17
- 239000004576 sand Substances 0.000 claims description 10
- 239000013589 supplement Substances 0.000 claims description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 230000006378 damage Effects 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 238000009395 breeding Methods 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 241000607479 Yersinia pestis Species 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 239000012531 culture fluid Substances 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 2
- 230000036541 health Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 30
- 241000196324 Embryophyta Species 0.000 description 11
- 229910052751 metal Inorganic materials 0.000 description 7
- 239000002184 metal Substances 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 159000000007 calcium salts Chemical class 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 229910002651 NO3 Inorganic materials 0.000 description 3
- 239000010949 copper Substances 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical group [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 239000012266 salt solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 238000001479 atomic absorption spectroscopy Methods 0.000 description 2
- 229910052793 cadmium Inorganic materials 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 150000002505 iron Chemical class 0.000 description 2
- 229910052745 lead Inorganic materials 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
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- 238000010672 photosynthesis Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
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- 230000005540 biological transmission Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Inorganic materials [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
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- 210000002421 cell wall Anatomy 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000000701 coagulant Substances 0.000 description 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 1
- 229940097267 cobaltous chloride Drugs 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
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- 238000005342 ion exchange Methods 0.000 description 1
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
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- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000003567 photophosphorylation Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
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- 239000010865 sewage Substances 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011684 sodium molybdate Substances 0.000 description 1
- 235000015393 sodium molybdate Nutrition 0.000 description 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
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- 239000011701 zinc Substances 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of method going heavy metal in eliminating water using Properties of Mangrove Seedlings; it is characterized in that the Mangrove hypocotyl in mangrove swamps protected area collection health; then it is placed in the husky base of Hoagland ' the s nutritional solution containing improvement and cultivates, in incubation, add certain density CaCl2Or Ca (NO3)2Solution, can be used for Cu in water after cultivating into Properties of Mangrove Seedlings under the conditions of suitable temperature and pH2+、Pb2+、Zn2+、Cd2+Remove etc. the absorption of heavy metal ion.The present invention is ensureing that heavy metal has the advantages of higher removal efficiency, non-secondary pollution under conditions of Properties of Mangrove Seedlings survives, and has extraordinary ecological benefits, development prospect is wide.
Description
Technical field
The present invention relates to a kind of method removing removing heavy metals using water plant, go in eliminating water in particular with Properties of Mangrove Seedlings
The method of heavy metal.
Background technology
In recent years, the construction with heavy industry of bordering on the sea and operation, the pollutant of a large amount of discharges come together in river mouth, bay area,
China's immediate offshore area heavy metal pollution is on the rise, such as in the North Sea, Qiezhou, the annual water quality report of Port of Fangcheng, all has weight
Metal is exceeded.Heavy metal pollution can lead to biological internal Accumulation of heavy metals serious, the accumulation of Cu, Pb, Cd and Hg in shell-fish body
Problem is serious, Cd and Pb content equals or exceeds human consumption standard value, and the biological internal Cd of software class exceedes human consumption standard
Value.These heavy metal contaminants can constantly be enriched in biology in vivo by food chain, and biological and human health are had greatly
Harm.
Traditional heavy metals removal method is mostly physics, chemical method, wherein the most commonly used with coagulant sedimentation, the party
Method is that heavy metal is converted into insoluble matter, need to use substantial amounts of chemical agent, and processing cost is high and can produce when processing not in time
Secondary pollution；Divide in addition with oxidation-reduction method, ion exchange, high molecular heavy metals trapping agent method, absorption method, solvent extraction
From method, membrane filter technique, evaporation etc., wherein larger application potential is had to receive much concern in terms of environmental protection with biosorption process.Raw
Thing absorption method be enriched with from aqueous solution using biological adsorption agents such as microorganisms, separation of heavy metal ions method, be a kind of preferable
Process water pollutant candidate material.The biological adsorption agent of early stage refers mainly to microorganism, the research of current biological adsorption agent
Scope has been not limited only to microorganism, can be the abiotic biological substance such as animals and plants fragment or the botanical system lived.
Rhizophora apiculata Blume is that a class is grown in the torrid zone, the xylophyta of subtropical zones seashore Intertidal zone, is mainly distributed on hidden bay
And in the alluviation mud of estuary.Rhizophora apiculata Blume is the distinctive ecotype of wetland, not only has higher economic worth, also powerful
Ecological functions, to safeguarding and improving bay, Hekou Area ecological environment, resist the natural disasters such as tide, stormy waves, preventing and treating is coastal
Marine pollution and the coastal Wetland Biodiversity of protection etc. have irreplaceable effect, and wherein of greatest concern is Rhizophora apiculata Blume
In purifying water body, improve effect in terms of inshore ecological environment.Researcher both domestic and external thinks that mangrove wetland can
Filter organic substance and the ecological functions of other pollutant that land runoff is taken out of, there is potential purification sewage ability.Rhizophora apiculata Blume
Plant has the root system of prosperity, and not only heavy metal has higher toleration, and heavy metal pollutant also have precipitation and inhale
Attached effect, goes heavy metal in eliminating water to have very big development potentiality hence with Rhizophora apiculata Blume as biological adsorption agent.
However, heavy metal in plant body be accumulated over finite concentration after, the ultrastructure of plant cell will be made
Become to damage, lead to the biochemical functions that these organelles participate in limited or even lose, ultimately result in apoptosis.Ca is plant
Carry out nutrient necessary to photosynthesis, not only involved in plant, from the whole life cycle growing into aging, and participates in
The physiological process such as photosynthesis of plant electron transmission and photophosphorylation, the tropic movement of cell and hormone regulating and controlling.Additionally, Ca is also
The growth of cell with the structure of stabilizing cell membrane, cell wall, can be promoted, improve enzyme activity and adjusting film ion Osmotic balance etc., and
Play an important role in plant signaling transduction as second message,second messenger.Therefore, by adding one during growing in Properties of Mangrove Seedlings
Quantitative Ca2+, and carry out condition control in the stage removing removing heavy metals, Properties of Mangrove Seedlings can be improved and go heavy metal in eliminating water
Efficiency simultaneously improves the survival rate of seedling, thus has boundless application prospect.
Content of the invention
The purpose of the present invention is for overcoming the deficiencies in the prior art, providing a kind of new method going heavy metal in eliminating water, main
Technical thought is wanted to be live body Properties of Mangrove Seedlings to be optimized heavy metal in the water body planted after CMC model containing heavy metal carry out
Absorb and remove.
The technical solution used in the present invention is as follows：
A kind of method going heavy metal in eliminating water using Properties of Mangrove Seedlings, comprises the following steps：
A. cultivate husky base pretreatment：Uncovered square container is put into, the height of Sha Ji is maintained at after fluvial sand is washed
20cm～25cm, then pours improvement Hoagland ' s nutritional solution (hereinafter referred to as nutritional solution), the height of nutritional solution in Sha Jizhong
Do not had husky base 3cm～5cm；
B. Properties of Mangrove Seedlings selection-breeding：From Rhizophora apiculata Blume, collection vitality is strong, no disease and pests harm and quality and the close plumular axis of size,
Cultivation Sha Jizhong by its neat inserting step a preparation；
C. Properties of Mangrove Seedlings culture：Add CaCl in the nutritional solution in the husky base of cultivation2Or Ca (NO3)2Soluble calcium salt is molten
Liquid, makes Ca in cultivation sand base2+Concentration reaches 300mg/L～600mg/L, culture period 12 months, in good time Replacement Battalion in incubation
Nutrient solution is to keep liquid level, and supplements solubility calcium saline solution of the same race in good time, makes Ca2+Concentration keeps stable；
D. Properties of Mangrove Seedlings is transplanted and Metal uptake：Will be net for the Properties of Mangrove Seedlings root sand washing after the completion of culture, it is transplanted to and contain
There is Cu2+、Pb2+、Zn2+、Cd2+In any one above heavy metal ion improvement Hoagland ' s nutritional solution in, Transplanting Density is every
1000ml nutritional solution is planted into four plants of Properties of Mangrove Seedlings, controls concentration of heavy metal ion in nutritional solution, makes Cd2+Ion concentration is in 2.0mg/
Below L, in below 20mg/L, pH value is adjusted to 5.5～6.0 to other heavy metal ion, and temperature is maintained at 20 DEG C～30 DEG C, mends in good time
Fill culture fluid, keep nutritional solution total amount and components unchanged, culture can measure the absorbtivity of heavy metal after 30 days～40 days.
Improvement Hoagland ' s nutritional solution described in above-mentioned steps, its formula is four water-calcium nitrate 945mg/L, potassium nitrate
506mg/L, ammonium nitrate 80mg/L, potassium dihydrogen phosphate 136mg/L, magnesium sulfate 493mg/L, iron salt solutions 2.5ml, liquid microelement
5ml, pH=6.0.The formula of wherein iron salt solutions is ferrous sulfate heptahydrate 2.78g, disodiumedetate (EDTA.Na)
3.73g, distilled water 500ml, pH=5.5；The formula of liquid microelement is potassium iodide 0.83mg/L, boric acid 6.2mg/L, manganese sulfate
22.3mg/L, zinc sulfate 8.6mg/L, sodium molybdate 0.25mg/L, copper sulfate 0.025mg/L, cobaltous chloride 0.025mg/L.
Soluble calcium salt solution C a in described step c2+Concentration is preferably 500mg/L.
Metal uptake amount is represented with the ratio of Properties of Mangrove Seedlings quality with the heavy metal quality absorbing, a wherein huge sum of money for absorption
Belong to quality and can adopt atomic absorption spectrometry, the quality of Properties of Mangrove Seedlings is in terms of dry weight.
Compared to existing technology, the substantive distinguishing features that the present invention projects and significantly progress are：
The invention provides a kind of new method going heavy metal in eliminating water using Properties of Mangrove Seedlings, invent using improvement
The method that Hoagland ' s nutritional solution and soluble calcium salt are optimized culture to Properties of Mangrove Seedlings, significantly improves Properties of Mangrove Seedlings and exists
Survival ability under the conditions of heavy metal stress and Metal uptake ability, and give preferred calcium salt culture concentration, base simultaneously
In the tolerance of Properties of Mangrove Seedlings, give the highest Limited Doses of heavy metal concentration.Technical solution of the present invention is simple, removes
Effect is significant, non-secondary pollution, is enriching further and perfect to prior art.
Specific embodiment
Embodiment 1：
A kind of method going heavy metal in eliminating water using Properties of Mangrove Seedlings, comprises the following steps：
A. cultivate husky base pretreatment：Plastics square box is put into, the height of Sha Ji is 20cm- after fluvial sand tap water is cleaned
25cm.Then improvement Hoagland ' s nutritional solution, the excessively not husky base about 3cm-5cm of height of nutritional solution are poured in Sha Jizhong.Equally
Cultivation sand base prepare 7, number respectively.
B. Properties of Mangrove Seedlings selection-breeding：From Mangrove Nature Reserves collection vitality is strong, no disease and pests harm and quality and size phase
Near plumular axis, then by the culture Sha Jizhong of inserting step a preparation neat for Mangrove hypocotyl.Selection-breeding quantity meets subsequent step need
Will.
C. Properties of Mangrove Seedlings culture：Add CaCl in culture sand base improvement Hoagland ' the s nutritional solution of step b2Solution,
Make Ca in the husky base of each culture2+Concentration be respectively 300mg/L, 350mg/L, 400mg/L, 450mg/L, 500mg/L, 550mg/L,
600mg/L.Supplement weekly in incubation and once improve Hoagland ' s nutritional solution, make total amount and the components unchanged of nutritional solution,
Supplement a CaCl every other month2Solution, makes Ca2+Concentration keeps constant.Culture period is 12 months.
D. Properties of Mangrove Seedlings is transplanted and Metal uptake：By the Properties of Mangrove Seedlings root sand washing after the completion of cultivating in husky for each culture base
Only, it is transplanted into respectively in the another seven plastics square boxes numbering and being loaded with improvement Hoagland ' s nutritional solution respectively, Transplanting Density
Plant into four plants of Properties of Mangrove Seedlings by every 1000ml nutritional solution；Transplanting adds toward in nutritional solution after finishing again and contains Cu2+、Pb2+、Zn2+、Cd2+
Etc. the solution of heavy metal ion, ion concentration is respectively 20mg/L, 20mg/L, 20mg/L, 2.0mg/L.PH is adjusted to 5.5-6.0,
Temperature is maintained at 20 DEG C -30 DEG C, and every 3 days supplements once improve Hoagland ' s nutritional solution, keeps nutritional solution total amount and composition not
Become, cultivate 30-40 days, Properties of Mangrove Seedlings growing way keeps good.
E. heavy metal ion absorbtivity measures：Using atomic absorption spectrometry.Measure numerical value as shown in table 1.
Table 1. CaCl2Different Ca during solution optimization culture2+Heavy metal ion absorbtivity under concentration
Embodiment 2
Essentially identical with embodiment 1 step, different is simply by CaCl2Replace with Ca (NO3)2.Metal uptake amount
As shown in table 2.
Table 2. Ca (NO3)2Different Ca during solution optimization culture2+Heavy metal ion absorbtivity under concentration
Embodiment 3
Essentially identical with embodiment 1 step, different is Ca in step c2+Concentration be 500mg/L, in step d
Cu2+、Pb2+、Zn2+、Cd2+The concentration of heavy metal ion is respectively 25mg/L, 25mg/L, 25mg/L, 2.5mg/L, and Properties of Mangrove Seedlings is trained
Start appearance when supporting the 7th day withered, start within the 9th day that fallen leaves phenomenon occurs, then wither further to death.
Embodiment 4
Essentially identical with embodiment 2 step, different is Cu in step d2+、Pb2+、Zn2+、Cd2+Heavy metal ion
Concentration is respectively 25mg/L, 25mg/L, 25mg/L, 2.5mg/L, and Properties of Mangrove Seedlings starts appearance and withers when cultivating the 8th day, the 10th day
Start that fallen leaves phenomenon occurs, then wither further to death.
Embodiment 5
Substantially the same manner as Example 1, different is in the culture of Properties of Mangrove Seedlings only using improvement Hoagland ' s battalion
Nutrient solution is not added with solubility calcium saline solution, in Metal uptake add containing Cu2+、Pb2+、Zn2+、Cd2+Etc. heavy metal ion solution
Ion concentration be respectively 10.0mg/L, 10.0mg/L, 10.0mg/L, 1.0mg/L, culture 20 days after Properties of Mangrove Seedlings occur fallen leaves
Phenomenon, continued culture to 35 days.Record Properties of Mangrove Seedlings after testing afterwards to Cu2+、Pb2+、Zn2+、Cd2+Absorption etc. heavy metal ion
Amount is respectively：Cu 662.19ug/g；Pb 969.71ug/g；；Zn 209.22ug/g；Cd 41.00ug/g.
Claims (3)
1. a kind of go the method for heavy metal in eliminating water it is characterised in that comprising the following steps using Properties of Mangrove Seedlings：
A. cultivate husky base pretreatment：Put into uncovered square container after fluvial sand is washed, the height of Sha Ji be maintained at 20cm～
25cm, then pours improvement Hoagland ' s nutritional solution, the excessively not husky base 3cm～5cm of height of nutritional solution in Sha Jizhong；
B. Properties of Mangrove Seedlings selection-breeding：From Rhizophora apiculata Blume, collection vitality is strong, no disease and pests harm and quality and the close plumular axis of size, by it
The cultivation Sha Jizhong of neat inserting step a preparation；
C. Properties of Mangrove Seedlings culture：Add solubility calcium saline solution in the nutritional solution in the husky base of cultivation, make Ca in cultivation sand base2+Dense
Scale standard reaches 300mg/L～600mg/L, Properties of Mangrove Seedlings is cultivated 12 months, in incubation, in good time Additional nutrient solution is to protect
Hold liquid level, and supplement solubility calcium saline solution of the same race in good time, make Ca2+Concentration keeps up to standard；
D. Properties of Mangrove Seedlings is transplanted and is absorbed：Will be net for the Properties of Mangrove Seedlings root sand washing after the completion of culture, it is transplanted to containing Cu2+、Pb2+、Zn2 +、Cd2+In the nutritional solution of heavy metal ion, control concentration of heavy metal ion in nutritional solution, make Cd2+Ion concentration 2.0mg/L with
Under, in below 20mg/L, pH value is adjusted to 5.5～6.0 to other heavy metal ion, and temperature is maintained at 20 DEG C～30 DEG C, supplements in good time
Culture fluid, keeps nutritional solution total amount and components unchanged, and culture can measure the absorbtivity of heavy metal after 30 days～40 days.
2. as claimed in claim 1 go the method for heavy metal in eliminating water using Properties of Mangrove Seedlings it is characterised in that described in step b
Solubility calcium saline solution be CaCl2Or Ca (NO3)2Solution.
3. as claimed in claim 1 go the method for heavy metal in eliminating water using Properties of Mangrove Seedlings it is characterised in that described in step c
Ca in the husky base nutritional solution of cultivation2+Concentration is 500mg/L.
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2015
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| CN104710016A (en) | 2015-06-17 |
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