CN104706896B - A kind of autonomic drug for the treatment of cancer and preparation method and application - Google Patents
A kind of autonomic drug for the treatment of cancer and preparation method and application Download PDFInfo
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- CN104706896B CN104706896B CN201510113850.7A CN201510113850A CN104706896B CN 104706896 B CN104706896 B CN 104706896B CN 201510113850 A CN201510113850 A CN 201510113850A CN 104706896 B CN104706896 B CN 104706896B
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Abstract
Rhizome of Forrest Paris [Paris forrestii (Takht.) H.Li] extract, its preparation method or its application in treatment tumour or cancer drug is prepared are provided.Rhizome of Forrest Paris extract, it is the rhizome extract extracted with ethanol, takes Rhizome of Forrest Paris rhizome, is soaked 7 days with 95% ethanol after dry, pulverize, and filtering, filter residue continues to extract, and extracts 4 times altogether, merging filtrate, recycling design, obtains extract medicinal extract.Rhizome of Forrest Paris extract has highly significant inhibitory activity to the cells of liver cancer SMMC 7721, the cells of lung cancer A 549, the cells of breast cancer MCF 7 and colon cancer SW480 cells, and its activity is better than positive control medicine cis-platinum.To treat or prevent the new selection of cancer drug.
Description
Technical field：
The invention belongs to pharmaceutical technology field, in particular it relates to autonomic drug technical field, more particularly, to long column weight
Building extract and preparation method thereof and its medicine for being used to prepare treating cancer,.
Background technology：
Rhizome of Forrest Paris [Paris forrestii (Takht.) H.Li] is Trilliaceae paris plant, and its rhizome is used as medicine,
The effect of with clearing heat and detoxicating, swelling and pain relieving, cool liver arresting convulsion, for treating ulcer sores, nameless sores or boils, venomous snake bite, throat
Swelling and pain, parotitis, tonsillitis and infection with swollen head (natural drug illustrated handbook Kunming of volume 6, Zhu Zhao cloud Yunnan:Yunnan sci-tech publication
Society, 2010:127), but at present still without for the report in terms for the treatment of cancer.
The content of the invention：
It is an object of the invention to provide Rhizome of Forrest Paris extract, its preparation method or its preparing treatment tumour or cancer medicine
Application in thing.
In order to realize the above-mentioned purpose of the present invention, the invention provides following technical scheme：
Rhizome of Forrest Paris extract, it is the rhizome extract extracted with ethanol.
According to described Rhizome of Forrest Paris extract, it is to take Rhizome of Forrest Paris rhizome, is soaked after dry, pulverize with 95% ethanol
7 days, filtering, filter residue continued to extract, and extracts 4 times altogether, merging filtrate, recycling design, obtains extract medicinal extract.
According to described Rhizome of Forrest Paris extract, it is to take Rhizome of Forrest Paris rhizome, is soaked after dry, pulverize with 95% ethanol
7 days, filtering, filter residue continued to extract, and extracts 4 times altogether, merging filtrate, recycling design, obtains extract medicinal extract；By extract medicinal extract
Suspension is made with water, is extracted with ethyl acetate, reclaims ethyl acetate, obtains Ethyl acetate fraction；After ethyl acetate extraction
Mother liquor, continue to use extracting n-butyl alcohol, reclaim n-butanol, obtain extracting n-butyl alcohol position；Mother liquor after extracting n-butyl alcohol, through D101
Macroporous resin column chromatography separates, and is first eluted with water, is evaporated water, obtains water elution position；Continue with 95% ethanol elution, to be evaporated molten
Agent, obtain alcohol elution.
According to described Rhizome of Forrest Paris extract, its be Rhizome of Forrest Paris extracting n-butyl alcohol position or macroreticular resin ethanol wash
De- position.
A kind of autonomic drug for preventing or treating tumour, it contains the described Rhizome of Forrest Paris extract and medicine of therapeutically effective amount
Acceptable carrier on.
The method for preparing described Rhizome of Forrest Paris extract, takes Rhizome of Forrest Paris rhizome, is soaked after dry, pulverize with 95% ethanol
Bubble 7 days, filtering, filter residue continue to extract, and extract 4 times altogether, merging filtrate, recycling design, obtain extract medicinal extract, extract is soaked
Suspension is made with water in cream, is extracted with ethyl acetate, and reclaims ethyl acetate, obtains Ethyl acetate fraction；Ethyl acetate extracts
Mother liquor afterwards, continue to use extracting n-butyl alcohol, reclaim n-butanol, obtain extracting n-butyl alcohol position；Mother liquor after extracting n-butyl alcohol, warp
D101 macroporous resin column chromatographies separate, and are first eluted with water, are evaporated water, obtain water elution position；Continue with 95% ethanol elution, to be evaporated
Solvent, obtain alcohol elution.
Application of the described Rhizome of Forrest Paris extract in the medicine for treating or preventing Cancerous disease is prepared.
According to application of the described Rhizome of Forrest Paris extract in the medicine for treating or preventing Cancerous disease is prepared, described in it
Cancer for liver cancer, lung cancer, breast cancer, colon cancer.
Extracting n-butyl alcohol position or macroreticular resin alcohol elution in described Rhizome of Forrest Paris extract are controlled in preparation
Application in the medicine for the treatment of or prevention Cancerous disease.
A kind of application of autonomic drug for preventing or treating tumour in the medicine for treating or preventing Cancerous disease is prepared, it is described
Cancer for liver cancer, lung cancer, breast cancer, colon cancer.
Compared with prior art, the present invention has following excellent beneficial effects：Rhizome of Forrest Paris extract, particularly n-butanol extract
Take position (PFE-PT3) and macroreticular resin alcohol elution (PFE-PT5) thin to SMMC-7721 liver cancer cells, lung cancer A-549
Born of the same parents, MCF-7 Breast Cancer Cell and colon cancer SW480 cells have highly significant inhibitory activity, and its activity is better than positive control drug
Thing cis-platinum.
The medicine of the present invention that tumour or cancer are prevented and treated using Rhizome of Forrest Paris and its extract as raw material, be
On the basis of prior art, any pharmaceutically useful preparation is prepared into using Chinese medicine preparation conventional method.For example, by Rhizome of Forrest Paris system
Taken after mixing it with water into powder；Tablet, capsule, granule, pill is made in extract, but this is not intended to limit protection scope of the present invention.
Extraction of the present invention, it can be extracted by heating extraction, normal temperature extraction or low temperature；With water, ethanol, methanol or
Other solvent refluxings, dipping or diacolation.Macroporous resin column chromatography of the present invention, can be the macroreticular resin of various models,
Eluant, eluent can be that water, ethanol, methanol or acetone mix in varing proportions.
Embodiment：
Further illustrate the essentiality content of the present invention with embodiments of the invention below, but this is not limited with this
Invention.
Embodiment 1：
Rhizome of Forrest Paris [Paris forrestii (Takht.) H.Li] dry rhizome (4.4kg), with 95% ethanol after crushing
(13L) soaks 7 days, and filtering, filter residue continues to extract.Extract 4 times altogether, merging filtrate, recycling design, obtain medicinal extract (numbering PFE-
PT1)550g.Suspension is made with water (2L) in PFE-PT1, is extracted with ethyl acetate (2L × 3), ethyl acetate is reclaimed, obtains second
Acetoacetic ester extracts position (numbering PFE-PT2) 19g.Mother liquor after ethyl acetate extraction, continues to use extracting n-butyl alcohol (2L × 3),
N-butanol is reclaimed, obtains extracting n-butyl alcohol position (numbering PFE-PT3) 290g.Mother liquor after extracting n-butyl alcohol, through D101 macropore trees
Fat column chromatography for separation, is first eluted with water, and is evaporated water, obtains water elution position (numbering PFE-PT4) 174g.Continue to be washed with 95% ethanol
It is de-, solvent evaporated, obtain alcohol elution (numbering PFE-PT5) 40g.
Embodiment 2：
Rhizome of Forrest Paris extract and treated each position are subjected to cytotoxic activity test, the results are shown in Table 1.As a result table
Bright, in addition to macroreticular resin water elution position (PFE-PT4) is without activity, there is cytotoxic activity at remaining each position, especially
Extracting n-butyl alcohol position (PFE-PT3) and macroreticular resin alcohol elution (PFE-PT5) are to SMMC-7721 liver cancer cells, lung
Cancer A-549 cells, MCF-7 Breast Cancer Cell and colon cancer SW480 cells have highly significant inhibitory activity, and its activity is better than sun
Property control drug cis-platinum.
The Rhizome of Forrest Paris extract of table 1 and each position are to growth of tumour cell inhibitory action
Embodiment 3：
Rhizome of Forrest Paris extract (PFE-PT1), sodium carboxymethyl starch, appropriate starch are added, gelatinized corn starch granulation, is dried, it is whole
Grain, addition talcum powder is appropriate, mixes, tabletting (every 0.5g, equivalent to Rhizome of Forrest Paris rhizome 1g), 6 tablets once, 3 times a day.
Embodiment 4：
The Ethyl acetate fraction (PFE-PT2) of Rhizome of Forrest Paris extract, sodium carboxymethyl starch, appropriate starch are added,
Gelatinized corn starch is pelletized, and is dried, whole grain, and addition talcum powder is appropriate, is mixed, tabletting (every 0.5g, equivalent to Rhizome of Forrest Paris rhizome 1g),
6 tablets once, 3 times a day.
Embodiment 5：
The extracting n-butyl alcohol position (PFE-PT3) of Rhizome of Forrest Paris extract, sodium carboxymethyl starch, appropriate starch are added, formed sediment
Paste mixing is pelletized, and is dried, whole grain, and addition talcum powder is appropriate, is mixed, tabletting (every 0.5g, equivalent to Rhizome of Forrest Paris rhizome 3g), often
Secondary 2,3 times a day.
Embodiment 6：
The macroreticular resin alcohol elution (PFE-PT5) of Rhizome of Forrest Paris extract, add sodium carboxymethyl starch, starch is fitted
Amount, gelatinized corn starch granulation, is dried, whole grain, and addition talcum powder is appropriate, is mixed, tabletting (every 0.5g, equivalent to Rhizome of Forrest Paris rhizome
3g), 2 tablets once, 3 times a day.
Claims (2)
1. a kind of prevention or treatment liver cancer, lung cancer, breast cancer, colon cancer, the autonomic drug of leukaemia, it is characterised in that it is by treating
The extracting n-butyl alcohol position PFE-PT3 or macroreticular resin alcohol elution PFE- of the Rhizome of Forrest Paris ethanol extract of effective dose
PT5, and pharmaceutically acceptable carrier are formed, and described extracting n-butyl alcohol position PFE-PT3 or macroreticular resin ethanol are washed
De- position PFE-PT5 is prepared by following methods：Rhizome of Forrest Paris rhizome is taken, is soaked 7 days with 95% ethanol after dry, pulverize,
Filtering, filter residue continue to extract, and extract 4 times altogether, merging filtrate, recycling design, obtain extract medicinal extract；By extract medicinal extract water
Suspension is made, is extracted with ethyl acetate, reclaims ethyl acetate, obtains Ethyl acetate fraction；Mother after ethyl acetate extraction
Liquid, continue to use extracting n-butyl alcohol, reclaim n-butanol, obtain extracting n-butyl alcohol position PFE-PT3；Mother liquor after extracting n-butyl alcohol, warp
D101 macroporous resin column chromatographies separate, and are first eluted with water, are evaporated water, obtain water elution position；Continue with 95% ethanol elution, to be evaporated
Solvent, obtain alcohol elution PFE-PT5.
2. the extracting n-butyl alcohol position PFE-PT3 or macroreticular resin alcohol elution PFE-PT5 of Rhizome of Forrest Paris ethanol extract
Application in the medicine for treating or preventing liver cancer, lung cancer, breast cancer, colon cancer, leukaemia is prepared, it is characterised in that described
Extracting n-butyl alcohol position PFE-PT3 or macroreticular resin alcohol elution PFE-PT5 are prepared by following methods：Take long column weight
Building rhizome, soaked 7 days with 95% ethanol after dry, pulverize, filtering, filter residue continues to extract, and extracts 4 times altogether, merging filtrate, returns
Solvent is received, obtains extract medicinal extract；Suspension is made with water in extract medicinal extract, is extracted with ethyl acetate, ethyl acetate is reclaimed, obtains
Ethyl acetate fraction；Mother liquor after ethyl acetate extraction, continue to use extracting n-butyl alcohol, reclaim n-butanol, obtain n-butanol extraction
Take position PFE-PT3；Mother liquor after extracting n-butyl alcohol, separated through D101 macroporous resin column chromatographies, be first eluted with water, be evaporated water,
Obtain water elution position；Continue with 95% ethanol elution, solvent evaporated, to obtain alcohol elution PFE-PT5.
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| CN105106556B (en) * | 2015-09-11 | 2018-08-10 | 中国科学院昆明植物研究所 | The chemical composition of Rhizome of Forrest Paris active anticancer position PFE-PT3 detaches and identification method |
| CN107446014B (en) * | 2017-08-04 | 2019-08-02 | 中国科学院昆明植物研究所 | Dulong Paris polyphylla ethanol extract and its application in pharmacy separate and identification method with its chemical component |
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| JP4918201B2 (en) * | 2001-09-13 | 2012-04-18 | 株式会社 日本薬用食品研究所 | Diabetes prevention and treatment |
| CN101904968B (en) * | 2010-07-27 | 2011-11-09 | 天津大学 | Preparation method of Chinese paris rhizome yam type saponin and anti-tumor medicinal preparation thereof |
| CN102139037A (en) * | 2011-03-30 | 2011-08-03 | 天津大学 | Application of rhizoma paridis total saponins in preparation of adjuvant drugs of anti-cancer drugs |
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