CN104706665A - Method for inhibiting aggregation of Abeta by using WS2 nanosheet and method for de-aggregating formed Abeta fiber aggregate - Google Patents
Method for inhibiting aggregation of Abeta by using WS2 nanosheet and method for de-aggregating formed Abeta fiber aggregate Download PDFInfo
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Abstract
The invention provides a method for inhibiting aggregation of Abeta by using a WS2 nanosheet and a method for de-aggregating formed Abeta fiber aggregate and belongs to the technical field of medicines. The method is used for inhibiting the aggregation of Abeta by utilizing a two-dimensional transition metal tungsten disulfide nanometer material; the WS2 nanosheet can be used for adsorbing an Abeta monomer by a Van Der Waals force between a planar base of the WS2 nanosheet and aromatic amino acids; meanwhile, the WS2 surface has negative charges and can be electrostatically combined with an Abeta positive ion group area to enhance the combination between the Abeta monomer and the WS2 sheet structure so as to inhibit the aggregation of the Abeta; the invention also provides a method for de-aggregating the formed Abeta fiber aggregate by using the WS2 nanosheet. The WS2 nanosheet has an obvious photo-thermal effect and can be used for photo-thermally de-aggregating the formed Abeta fiber aggregate under the illumination of near infrared light; meanwhile, the WS2 nanosheet has the effect of alleviating the cytotoxicity caused by Abeta.
Description
Technical field
The invention belongs to medical art, be specifically related to one WS
2nanometer sheet suppresses the method for A beta peptide aggregation and the method for depolymerization established A beta aggregation.
Background technology
Alzheimer (AD) is one of the most common form of senile dementia.The clinical symptoms main manifestations of AD patient is cognitive dysfunction, gradual memory goes down, abnormality of personality etc.Its pathological characteristics is that the outer senile plaque deposition of brain inner cell and cellular neurofibrillary tangle.Wherein the main component of senile plaque is beta amyloid peptide (amyloid β-peptide, A β).The experimental results shows that the excessive generation of A β and gathering are the key pathological events of AD.Suppress the Assembling Behavior of A β and depolymerization established A beta aggregation is the effective way of prevention and treatment AD.A beta peptide aggregation process can be regulated and controled and the material reducing A β toxicity is the very promising drug candidate for the treatment of AD.
Based on this, a large amount of peptide derivatives, organic molecule and some metal complexs are designed to synthesize the established A beta of gathering or depolymerization for suppressing A β.But because its targeting is poor, blood brain barrier (BBB) permeability is low and toxic and side effects comparatively high, most inhibitor only shows medium inhibition and faint depolymerization ability.Therefore in the urgent need to designing novel compound or material for suppressing A beta peptide aggregation and depolymerization A beta aggregation.In recent years, nano material gets more and more people's extensive concerning day by day because of its larger specific surface area and the character such as unique optical, electrical, magnetics and catalytic activity.Nanosecond science and technology merge with life sciences by nanobiology mutually, become the focus of modern science and technology research, have a good application prospect at numerous areas such as biomedicine, electronics, materialogy, environmental sciences.Large quantity research shows, nano material can pass through hydrophobic interaction, pi-pi accumulation effect, Van der Waals force and electrostatic interaction etc. and interaction of biomacromolecules.Nano material can as a kind of novel pharmaceutical agent for regulating and controlling the Assembling Behavior of A β thus treating AD.The multiple nano material of current discovery can suppress the gathering (Adv.Mater.2013,25,3780 – 3801.) of A β, but these nano materials mainly realize the regulating and controlling effect to the behavior of A beta peptide aggregation by the electric charge on its surface and group.
Summary of the invention
The object of the invention is to provide one WS
2nanometer sheet suppresses the method for A beta peptide aggregation and the method for depolymerization established A beta aggregation, and the method can greatly reduce the toxic and side effects of medicine and improve selectivity.
First the present invention provides one WS
2nanometer sheet suppresses the method for A beta peptide aggregation, and the method comprises:
Step one: preparation WS
2nanometer sheet;
Step 2: processed by A beta monomers sample, then by the A beta monomers sample after process and WS
2nanometer sheet, in buffer solution, is hatched at 37 DEG C and is suppressed A beta peptide aggregation.
Preferably, described to the method that A beta monomers sample processes is: by A β powder dissolution in hexafluoroisopropanol, shakes 2-6 hour, dried up by hexafluoroisopropanol by nitrogen current at being placed in 4 DEG C, then be dissolved in buffer solution, in 4 DEG C, leave standstill 6-12 hour.
Preferably, described buffer solution is 10mM HEPES, 150mM NaCl, pH 7.3.
The present invention also provides one WS
2the method of nanometer sheet depolymerization established A beta aggregation, the method comprises:
Step one: preparation WS
2nanometer sheet;
Step 2: processed by A beta monomers sample, is then dissolved in buffer by the A beta monomers sample after process, hatches 7 days, obtain A beta aggregation at 37 DEG C;
Step 3: by WS
2after nanometer sheet and A beta aggregation hatch 20 minutes at 37 DEG C, near-infrared laser illumination 5min, carries out depolymerization to established A beta aggregation.
Preferably, described to the method that A beta monomers sample processes is: by A β powder dissolution in hexafluoroisopropanol, shakes 2-6 hour, dried up by hexafluoroisopropanol by nitrogen current at being placed in 4 DEG C, then be dissolved in buffer solution, in 4 DEG C, leave standstill 6-12 hour.
Preferably, described buffer solution is 10mM HEPES, 150mM NaCl, pH 7.3.
Beneficial effect of the present invention
First the present invention provides one WS
2nanometer sheet suppresses the method for A beta peptide aggregation, and the method utilizes transition metal tungsten disulfide (WS
2) two-dimension nano materials suppresses the gathering of A β, WS
2nanometer sheet can adsorb A beta monomers by the Van der Waals force between its planar substrates and aromatic amino acid, meanwhile, and WS
2surface band negative electricity, can the cation bunch region of electrostatical binding A β, strengthens A beta monomers and WS
2combination between lamellar structure, suppresses A beta peptide aggregation; Experimental result shows: WS
2nanometer sheet can suppress A beta peptide aggregation significantly, and its inhibition is concentration dependent; WS
2still can realize in complex system is as bovine serum albumin (BSA) solution and mouse brain spinal fluid (CSF) the inhibitory action of A beta monomers.
The present invention also provides one WS
2the method of nanometer sheet depolymerization established A beta aggregation, WS
2nanometer sheet has higher optical absorption near infrared region, utilizes the character of its near infrared absorption, and can effectively melt cancerous cell and reduce the tumor size of mice by photo-thermal, based on this, the method utilizes WS
2nano-lamellar structure photo-thermal depolymerization established A beta aggregation, compare with radiation therapy with traditional chemotherapy, the advantage of thermotherapy is the diseased region just meeting heat production only having illumination, and other normal structure is unaffected, the toxic and side effects of medicine can greatly be reduced and improve selectivity.Experimental result shows: WS
2nanometer sheet has obvious photo-thermal effect, can effective photo-thermal depolymerization established A beta aggregation under near infrared light; Meanwhile, WS
2nanometer sheet can reduce the cytotoxicity that A β causes; WS
2still can realize in complex system is as bovine serum albumin (BSA) solution and mouse brain spinal fluid (CSF) the photo-thermal depolymerisation of A beta aggregation.
Accompanying drawing explanation
Fig. 1 is the WS of embodiment 1 step one synthesis
2nanometer sheet atom is tried hard to;
Fig. 2 is embodiment 1WS
2the inhibitory action ThT fluoroscopic examination figure that nanometer sheet is assembled A β 1-40;
Fig. 3 is embodiment 1A β 1-40 and WS
2nanometer sheet hatches the attenuated total reflectance infared spectrum after 7 days altogether;
Fig. 4 is embodiment 1A β 1-40 and WS
2nanometer sheet hatches the CD spectrogram after 7 days altogether;
Fig. 5 is embodiment 1WS
2nanometer sheet assembles the AFM shape appearance figure of impact to A β 1-40;
Fig. 6 is embodiment 2WS
2nanometer sheet is to the photo-thermal depolymerisation ThT fluoroscopic examination figure of A β 1-40 fiber aggregate;
Fig. 7 is embodiment 2WS
2nanometer sheet is to the CD spectrogram of the photo-thermal depolymerisation of A β 1-40 fiber aggregate;
Fig. 8 is embodiment 2WS
2nanometer sheet assembles the AFM shape appearance figure of impact to A β 1-40;
Fig. 9 is embodiment 3WS
2the inhibitory action ThT fluoroscopic examination figure that nanometer sheet is assembled A β 1-40;
Figure 10 is embodiment 4WS
2nanometer sheet is to the photo-thermal depolymerisation ThT fluoroscopic examination figure of A β 1-40 fiber aggregate;
Figure 11 mtt assay detects WS
2the cytotoxicity suppression figure that nanometer sheet causes A β 1-40.
Detailed description of the invention
First the present invention provides one WS
2nanometer sheet suppresses the method for A beta peptide aggregation, and the method comprises:
Step one: preparation WS
2nanometer sheet;
Step 2: processed by A beta monomers sample, then by the A beta monomers sample after process and WS
2nanometer sheet, in buffer solution, is hatched at 37 DEG C and is suppressed A beta peptide aggregation.
According to the present invention, first prepare WS
2nanometer sheet, WS
2the preparation method of nanometer sheet is the technical method that this area is commonly used, and is not particularly limited, and is preferably: in a nitrogen environment, by WS
2in a solvent, described solvent is n-butyllithium solution or hexane solution to powder dissolution, after 2 days, use filter paper filtering mixture, and with hexanes wash, described filter paper is preferably Whatman#41 filter paper, adds deionized water by the leather hard precipitate obtained, supersound process 1 hour, then by solution centrifugal, the bulky grain removed lithium ion with deionized water wash and do not disperse for 3 times, collects supernatant, dialyse 5 days with deionized water, obtain WS
2nanometer sheet.The WS obtained
2the average thickness of nanometer sheet is 1.7nm, and average diameter is 200nm.
According to the present invention, described is preferably the method that A beta monomers sample processes: by A β powder dissolution in hexafluoroisopropanol, 2-6 hour is shaken at being placed in 4 DEG C, it is made all to dissolve, storing solution is preserved at-20 DEG C, before the use, by nitrogen current, hexafluoroisopropanol is dried up, then be dissolved in buffer solution, in 4 DEG C, leave standstill 6-12 hour.Described buffer solution is preferably 10mM HEPES, 150mM NaCl, pH 7.3.
According to the present invention, by the A beta monomers sample after process and the WS of variable concentrations
2nanometer sheet, in buffer solution, is hatched at 37 DEG C and is suppressed A beta peptide aggregation.The concentration of described A beta monomers sample is 50 μMs, WS
2the concentration of nanometer sheet is 0-100 μ gmL
-1, described buffer solution is preferably 10mM HEPES, 150mM NaCl, pH 7.3.WS
2the rejection ability of A beta peptide aggregation is characterized by thioflavin T (ThT) fluorescence spectrum, circular dichroism spectra (CD), atomic force (AFM) and attenuated total reflectance infrared spectrum (ATR-FTIR) means.
WS
2nano-material surface, in conjunction with A beta monomers, can reduce the concentration of monomer in solution, and the fibrosis of A β and monomer concentration closely related, reduce monomer concentration can suppress A beta peptide aggregation.Meanwhile, the process that A beta monomers forms oligomer by intermolecular interaction is reversible.The reduction of monomer concentration can cause this reaction to be carried out to the direction of oligomer depolymerization, reduces the content of gathering core or the seed existed in solution, thus effectively suppresses its accumulation process.WS
2nanoscale twins by Van der Waals force and electrostatic interaction absorption A beta monomers, thus can reach the effect suppressing A beta peptide aggregation.
The present invention also provides one WS
2the method of nanometer sheet depolymerization established A beta aggregation, the method comprises:
Step one: preparation WS
2nanometer sheet;
Step 2: processed by A beta monomers sample, is then dissolved in buffer by the A beta monomers sample after process, hatches 7 days, obtain A beta aggregation at 37 DEG C;
Step 3: by WS
2after nanometer sheet and A beta aggregation hatch 20 minutes at 37 DEG C, near-infrared laser illumination 5min, carries out depolymerization to established A beta aggregation.
According to the present invention, first prepare WS
2nanometer sheet, WS
2the preparation method of nanometer sheet is the technical method that this area is commonly used, and is not particularly limited, and is preferably: in a nitrogen environment, by WS
2in a solvent, described solvent is n-butyllithium solution or hexane solution to powder dissolution, after 2 days, use filter paper filtering mixture, and with hexanes wash, described filter paper is preferably Whatman#41 filter paper, adds deionized water by the leather hard precipitate obtained, supersound process 1 hour, then by solution centrifugal, the bulky grain removed lithium ion with deionized water wash and do not disperse for 3 times, collects supernatant, dialyse 5 days with deionized water, obtain WS
2nanometer sheet.The WS obtained
2the average thickness of nanometer sheet is 1.6nm, and average diameter is 200nm.
According to the present invention, described is preferably the method that A beta monomers sample processes: by A β powder dissolution in hexafluoroisopropanol, 2-6 hour is shaken at being placed in 4 DEG C, it is made all to dissolve, storing solution is preserved at-20 DEG C, before the use, by nitrogen current, hexafluoroisopropanol is dried up, then be dissolved in buffer solution, in 4 DEG C, leave standstill 6-12 hour.Described buffer solution is preferably 10mM HEPES, 150mM NaCl, pH 7.3.
According to the present invention, the A beta monomers sample after above-mentioned process is dissolved in buffer, at 37 DEG C, hatches 7 days, obtain A beta aggregation; Described buffer solution is preferably 10mM HEPES, 150mM NaCl, pH 7.3.By WS
2after nanometer sheet and A beta aggregation hatch 20 minutes at 37 DEG C, near-infrared laser illumination 5min, carries out depolymerization to established A beta aggregation.Described near-infrared laser intensity is 1Wcm
-2; WS
2characterized by thioflavin T (ThT) fluorescence spectrum, circular dichroism spectra (CD), atomic force (AFM) and attenuated total reflectance infrared spectrum (ATR-FTIR) means the effect of photo-thermal depolymerization A beta aggregation, the Cytotoxic mitigation caused A beta peptide aggregation body is by MTT method and membranolysis simulation experiment explanation.
Below in conjunction with embodiment, the present invention will be further described in detail.
Embodiment 1
WS
2nanometer sheet suppresses the self aggregation of A β 1-40:
Step one: preparation WS
2nanometer sheet: in a nitrogen environment, the WS of 100mg
2powder dissolution reacts in 1.6M n-butyllithium solution, after 2 days, with Whatman#41 filter paper filtering mixture, and with 100mL hexanes wash 3 times, in the leather hard precipitate obtained, add 300mL deionized water, supersound process 1 hour, then by solution centrifugal, the bulky grain removed lithium ion with deionized water wash and do not disperse for 3 times, collects supernatant, dialyse 5 days with deionized water, obtain WS
2nanometer sheet;
Step 2: first by A β 1-40 (kinds of goods U10012, buy from American Peptide) be dissolved in hexafluoroisopropanol with the concentration of 1mg/ml, bottleneck is sealed, vibrate 2 hours at being placed in 4 DEG C, it is impelled all to dissolve, storing solution is preserved at-20 DEG C, before the use, by soft nitrogen current, hexafluoroisopropanol is dried up, then corresponding buffer solution (10mM HEPES is again dissolved in, 150mM NaCl, pH 7.3) in, in 4 DEG C of refrigerators, leave standstill balance 6 hours; By 50 μMs of A β 1-40 after process and variable concentrations (0-100 μ gmL
-1) WS
2nanometer sheet, in buffer solution, is hatched at 37 DEG C, and ThT fluorescence detection method analyzes WS
2the impact that nanometer sheet is assembled A β 1-40.
Fig. 1 is the WS of embodiment 1 step one synthesis
2nanometer sheet atom is tried hard to, and wherein scheming A is that atom is tried hard to, and figure B is corresponding height map, as can be seen from Figure 1, and the WS of synthesis
2nanometer sheet average thickness is 1.7nm, and average diameter is 200nm.
Fig. 2 is embodiment 1WS
2the inhibitory action ThT fluoroscopic examination figure that nanometer sheet is assembled A β 1-40; Fig. 2 illustrates that A β 1-40 is typical S type from the ThT Fluorescence Increasing curve of collecting process and incubation time, shows that it meets nucleation-dependency Aggregation Model, and WS
2nanometer sheet then suppresses A β 1-40 fibrotic processes in concentration dependent.
Fig. 3 is embodiment 1A β 1-40 and WS
2nanometer sheet hatches the attenuated total reflectance infared spectrum after 7 days altogether; Fig. 4 is embodiment 1A β 1-40 and WS
2nanometer sheet hatches the CD spectrogram after 7 days altogether, Fig. 3 and Fig. 4 show A β 1-40 via hatch aging after can change beta sheet structure into.And and WS
2after hatching altogether, A β 1-40 is still random coil structure.
Fig. 5 is embodiment 1WS
2nanometer sheet assembles the AFM shape appearance figure of impact to A β 1-40, wherein schemes the AFM shape appearance figure that A is A β 1-40, and figure B is A β 1-40 and WS
2the AFM shape appearance figure of mixture, Fig. 5 shows A β 1-40 WS
2after nanometer sheet effect, only have a small amount of random precipitation to produce, substantially do not observe aggregate structure, WS is described
2nanometer sheet can suppress the formation of A β 1-40 beta sheet structure effectively.
Embodiment 2
WS
2nanometer sheet photo-thermal depolymerization established A β 1-40 fiber aggregate:
Step one: preparation WS
2nanometer sheet: in a nitrogen environment, the WS of 100mg
2powder dissolution reacts in 1.6M n-butyllithium solution, after 2 days, with Whatman#41 filter paper filtering mixture, and with 100mL hexanes wash 3 times, in the leather hard precipitate obtained, add 300mL deionized water, supersound process 1 hour, then by solution centrifugal, the bulky grain removed lithium ion with deionized water wash and do not disperse for 3 times, collects supernatant, dialyse 5 days with deionized water, obtain WS
2nanometer sheet;
Step 2: first by A β 1-40 (kinds of goods U10012, buy from American Peptide) be dissolved in hexafluoroisopropanol with the concentration of 1mg/ml, bottleneck is sealed, vibrate 2 hours at being placed in 4 DEG C, it is impelled all to dissolve, storing solution is preserved at-20 DEG C, before the use, by soft nitrogen current, hexafluoroisopropanol is dried up, then corresponding buffer solution (10mM HEPES is again dissolved in, 150mM NaCl, pH 7.3) in, in 4 DEG C of refrigerators, leave standstill balance 6 hours; A β 1-40 sample after above-mentioned process is dissolved in buffer (10mM HEPES, 150mM NaCl, pH 7.3), at 37 DEG C, hatches 7 days, obtain A β 1-40 fiber aggregate;
Step 3: by 40 μ gmL
-1wS
2nanometer sheet and 50 μMs of A β 1-40 fiber aggregates in buffer (10mM HEPES, 150mM NaCl, pH 7.3), 37 DEG C hatch 20 minutes after, make WS
2the Targeting Effect of A β 1-40 fiber is maximized, uses 1Wcm
-2the laser of power density irradiates 5 minutes, carries out depolymerization to established A β 1-40 fiber aggregate.
Fig. 6 is embodiment 2WS
2nanometer sheet is to the photo-thermal depolymerisation ThT fluoroscopic examination figure of A β 1-40 fiber aggregate; Fig. 7 is embodiment 2WS
2nanometer sheet is to the CD spectrogram of the photo-thermal depolymerisation of A β 1-40 fiber aggregate; Fig. 8 is embodiment 2WS
2nanometer sheet assembles the AFM shape appearance figure of impact to A β 1-40, and wherein scheming A is at WS
2under nanometer sheet exists, the AFM shape appearance figure of A β 1-40 fiber aggregate, figure B is A β 1-40 fiber aggregate and WS after near infrared light photo-irradiation treatment
2after mixture, the AFM shape appearance figure of A β 1-40; Fig. 6-8 can illustrate, the β-lamellar structure in A β 1-40 drops to 22.4% from 54.8% before, and independent near-infrared irradiates or WS
2itself all can not the structure of depolymerization established A β 1-40 fiber aggregate.
Embodiment 3
WS
2nanometer sheet suppresses A β 1-40 to assemble in cerebrospinal fluid
Reaction condition and step are with embodiment 1, and difference is, by A β 1-40 and the WS after process
2nanometer sheet is hatched in cerebrospinal fluid.
Fig. 9 is embodiment 3WS
2the inhibitory action ThT fluoroscopic examination figure that nanometer sheet is assembled A β 1-40, Fig. 9 can find out, in cerebrospinal fluid, WS
2nano flake still can suppress A β 1-40 fibrotic processes in ground in concentration dependent.
Embodiment 4
WS
2nanometer sheet is photo-thermal depolymerization A β 1-40 fiber aggregate in cerebrospinal fluid
Reaction condition and step are with embodiment 2, and difference is, by WS
2nanometer sheet and A β 1-40 fiber aggregate are hatched in cerebrospinal fluid.
Figure 10 is embodiment 4WS
2nanometer sheet is to the photo-thermal depolymerisation ThT fluoroscopic examination figure of A β 1-40 fiber aggregate, and Figure 10 can find out, compared with A β 1-40 fiber aggregate, after the treatment with irradiation of near infrared light, fluorescence intensity drops to 58%.
Embodiment 5
WS
2nanometer sheet alleviates the cytotoxicity experiment that A β 1-40 causes
PC12 cell (Mus pheochromocytoma) is provided by American Type Culture Collecti (American Type CultureCollection).The IMDM culture medium (Gibco BRL) of being furnished with 5% hyclone and 10% horse serum, for the cultivation of cell, keeps moistening environment, constant temperature 37 DEG C, and passes to containing 5%CO in incubator
2air.PC12 cell is placed on 96 orifice plates with the hole density of 10000 cells, and hatches in incubator and within 24 hours, allow cell attachment and propagation.
Inhibition test:
50 μMs of A β 1-40 first with variable concentrations WS
2nanometer sheet (20 μ gmL
-1, 40 μ gmL
-1) sheet in vitro in buffer solution 37 DEG C hatch 7 days.By continuing cultivation 36 hours in A β 1-40 sample dispersion to the culture medium of PC12, (final concentration of A β 1-40 is 5 μMs, WS
2the final concentration of nanometer sheet is respectively 2 μ gmL
-1, 4 μ gmL
-1).
Depolymerization is tested: the WS of cell culture medium variable concentrations
2(final concentration is 2 μ gmL
-1, 4 μ gmL
-1) replace, and rapidly established A β 1-40 aggregation (final concentration is 5 μMs) is joined in cell.Then, orifice plate with 808nm near infrared laser with 0.5Wcm
-2power density irradiate 5 minutes.Test as a comparison, simultaneously will containing WS
2the PC12 cell of-A β 1-40 is positioned over dark place.After cell continues to hatch 36 hours, adding final concentration is 0.5mgmL
-1mTT continue cultivation 4 hours.Dissolve with DMSO after removing culture medium, measure the absorption value under 570nm and 630nm wavelength.
Figure 11 mtt assay detects WS
2the cytotoxicity suppression figure that nanometer sheet causes A β 1-40, wherein * P<0.05, * * P<0.01, * * * P<0.001., Figure 11 display, A β 1-40 fibril aggregation physical ability causes cytoactive to decline 44%.In Inhibition test, WS
2the toxicity that significantly can reduce A β is hatched altogether with A β 1-40, and the reduction of its toxicity and WS
2in concentration dependent.On the other hand, with the cell of A β 1-40 fiber aggregate process at WS
2effect is lower uses near infrared light 5 minutes, and the survival rate of cell is increased to 88% in experimental error, independent WS
2effect or laser irradiate and all have no significant effect the survival rate of cell.
Claims (6)
1. one kind with WS
2nanometer sheet suppresses the method for A beta peptide aggregation, and it is characterized in that, the method comprises:
Step one: preparation WS
2nanometer sheet;
Step 2: processed by A beta monomers sample, then by the A beta monomers sample after process and WS
2nanometer sheet, in buffer solution, is hatched at 37 DEG C and is suppressed A beta peptide aggregation.
2. one WS according to claim 1
2nanometer sheet suppresses the method for A beta peptide aggregation, it is characterized in that, described to the method that A beta monomers sample processes is: by A β powder dissolution in hexafluoroisopropanol, 2-6 hour is shaken at being placed in 4 DEG C, by nitrogen current, hexafluoroisopropanol is dried up, then be dissolved in buffer solution, in 4 DEG C, leave standstill 6-12 hour.
3. one WS according to claim 1
2nanometer sheet suppresses the method for A beta peptide aggregation, and it is characterized in that, described buffer solution is 10mM HEPES, 150mM NaCl, pH 7.3.
4. one kind with WS
2the method of nanometer sheet depolymerization established A beta aggregation, it is characterized in that, the method comprises:
Step one: preparation WS
2nanometer sheet;
Step 2: processed by A beta monomers sample, is then dissolved in buffer by the A beta monomers sample after process, hatches 7 days, obtain A beta aggregation at 37 DEG C;
Step 3: by WS
2after nanometer sheet and A beta aggregation hatch 20 minutes at 37 DEG C, near-infrared laser illumination 5min, carries out depolymerization to established A beta aggregation.
5. one WS according to claim 4
2the method of nanometer sheet depolymerization established A beta aggregation, it is characterized in that, described to the method that A beta monomers sample processes is: by A β powder dissolution in hexafluoroisopropanol, 2-6 hour is shaken at being placed in 4 DEG C, by nitrogen current, hexafluoroisopropanol is dried up, then be dissolved in buffer solution, in 4 DEG C, leave standstill 6-12 hour.
6. one WS according to claim 4
2the method of nanometer sheet depolymerization established A beta aggregation, is characterized in that, described buffer solution is 10mM HEPES, 150mM NaCl, pH 7.3.
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| CN106115786B (en) * | 2016-06-27 | 2017-09-15 | 中国地质大学（北京） | Tungsten disulfide nanosheet tubular aggregate and preparation method thereof |
| CN114681481A (en) * | 2021-09-30 | 2022-07-01 | 合肥工业大学 | Chiral two-dimensional sulfur nanosheet for selectively resisting gram-positive bacteria and preparation method and application thereof |
| CN114681481B (en) * | 2021-09-30 | 2023-05-02 | 合肥工业大学 | Chiral two-dimensional sulfur nano-sheet for selectively resisting gram-positive bacteria and preparation method and application thereof |
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