CN104689065B - Include charred RADIX ET RHIZOMA RHEI and Bletilla glucomannan medical composition and its use - Google Patents
Include charred RADIX ET RHIZOMA RHEI and Bletilla glucomannan medical composition and its use Download PDFInfo
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- CN104689065B CN104689065B CN201510032265.4A CN201510032265A CN104689065B CN 104689065 B CN104689065 B CN 104689065B CN 201510032265 A CN201510032265 A CN 201510032265A CN 104689065 B CN104689065 B CN 104689065B
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Abstract
The invention discloses one kind to include charred RADIX ET RHIZOMA RHEI and Bletilla glucomannan medical composition and its use, and the pharmaceutical composition includes charred RADIX ET RHIZOMA RHEI；And Bletilla glucomannan.The pharmaceutical composition has astringing to arrest bleeding, clearing away heat to stop bleeding and detumescence and promoting granulation and other effects.
Description
Technical field
The invention belongs to biopharmacy technical field, and specifically, the present invention relates to one kind to include charred RADIX ET RHIZOMA RHEI and Bletilla glucomannan
Medical composition and its use.
Background technology
Chinese medicine charcoal processing is long applied to clinical history, and rheum officinale hemostasis earliest documents are recorded in Han dynasty Zhang Zhongjing《The Treatise on Fevrile Diseases》.
Rheum officinale clinical application is in various blood troubles, and effective hemostatic compositions monomer is d- catechins and gallic acid, another rheum emodin, aloe rheum officinale
Element, Chrysophanol, Rhein etc. have inhibitory action to helicobacter pylori.Charred RADIX ET RHIZOMA RHEI is made using modern superfine communication technique
Micron rhubarb charcoal, improve absorption and the bioavilability of medicine to greatest extent again on the basis of original property of medicine is kept, simultaneously
Shorten the onset time of medicine.
The bletilla striata, pungent, bitter, sweet, puckery, be slightly cold, return lung, liver, stomach.There are hemostasis tonifying lung, the effect of myogenic pain relieving.The bletilla striata is rich viscous
Property, colloid containing the bletilla striata, i.e. bletilla mannan, can hyoscine, be also often made for auxiliary material, often match somebody with somebody with other modern high polymer materials
Close and use.
However, about having no report by the medicine that main ingredient, Bletilla glucomannan are adjuvant and main matrix of micron rhubarb charcoal.
The content of the invention
It is contemplated that at least solves one of technical problem in correlation technique to a certain extent.Therefore, the present invention
One purpose is that proposition one kind includes charred RADIX ET RHIZOMA RHEI and Bletilla glucomannan medical composition and its use, and the pharmaceutical composition has convergence
Hemostasis, clearing away heat to stop bleeding and detumescence and promoting granulation and other effects.
In one aspect of the invention, the present invention proposes a kind of pharmaceutical composition, including：
Charred RADIX ET RHIZOMA RHEI；And
Bletilla glucomannan.
Pharmaceutical composition according to embodiments of the present invention has astringing to arrest bleeding, clearing away heat to stop bleeding and detumescence and promoting granulation and other effects.
In addition, pharmaceutical composition according to the above embodiment of the present invention can also have technical characteristic additional as follows：
In some embodiments of the invention, the Bletilla glucomannan obtains through the following steps：(1) by bletilla striata medicinal material with
Water mixes, and is decocted, to obtain bletilla striata Aqueous extracts；And (2) mix the bletilla striata Aqueous extracts with ethanol, to obtain
Precipitation containing Bletilla glucomannan.Thus, it is possible to significantly improve the extraction efficiency of Bletilla glucomannan.
In some embodiments of the invention, in step (1), the weight ratio of the bletilla striata medicinal material and the water is 1:10
~20, optional, the weight ratio of the bletilla striata medicinal material and the water is 1:15.Thus, it is possible to further improve carrying for Bletilla glucomannan
Take efficiency.
In some embodiments of the invention, in step (1), repeatedly decocted, 2 hours every time, optional, carry out
Decoct three times, 2 hours every time.Thus, it is possible to further improve the extraction efficiency of Bletilla glucomannan.
In some embodiments of the invention, in step (2), after the bletilla striata Aqueous extracts are mixed with the ethanol,
Final concentration of 55~80 volume % of ethanol.Thus, it is possible to further improve the extraction efficiency of Bletilla glucomannan.
In some embodiments of the invention, in step (2), the bletilla striata Aqueous extracts are being mixed it with the ethanol
Before, the bletilla striata Aqueous extracts are concentrated in advance, wherein, according to 3~7:The 1 ratio progress concentration, optional, according to 5:1
Ratio carry out the concentration.Thus, it is possible to further improve the extraction efficiency of Bletilla glucomannan.
In some embodiments of the invention, in step (2), after the bletilla striata Aqueous extracts are mixed with the ethanol,
The final concentration of 70 volume % of ethanol, is filtered after standing overnight, filter residue is dried under reduced pressure to perseverance at 80 degrees celsius
Weight.Thus, it is possible to further improve the extraction efficiency of Bletilla glucomannan.
In some embodiments of the invention, described pharmaceutical composition contains：The charred RADIX ET RHIZOMA RHEI of 12~20 parts by weight is described big
The granularity of yellow charcoal is 15~45 μm；And 30~50 parts by weight bletilla striata sol solution, the concentration of the bletilla striata sol solution for 0.1~
0.2g/g,
Optional, the weight ratio of the charred RADIX ET RHIZOMA RHEI and bletilla striata crude drug in the bletilla striata sol solution is 1:2.
In another aspect of the present invention, the present invention proposes a kind of pharmaceutical composition recited above in medicine is prepared
Purposes, the medicine be used for stop blooding.
In addition, purposes of the pharmaceutical composition according to the above embodiment of the present invention in medicine is prepared can also have it is as follows
Additional technical characteristic：
In some embodiments of the invention, the medicine is used to treat Peptic Ulcer.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Embodiment
Embodiments of the invention are described below in detail, the embodiments described below is exemplary, it is intended to for explaining this
Invention, and be not considered as limiting the invention.
In one aspect of the invention, the present invention proposes a kind of pharmaceutical composition.According to an embodiment of the invention, the medicine
Compositions include charred RADIX ET RHIZOMA RHEI and Bletilla glucomannan.Inventor's discovery, the pharmaceutical composition as obtained by mixing charred RADIX ET RHIZOMA RHEI and Bletilla glucomannan
With significant astringing to arrest bleeding, clearing away heat to stop bleeding and detumescence and promoting granulation and other effects.
According to an embodiment of the invention, Bletilla glucomannan obtains through the following steps：(1) bletilla striata medicinal material is mixed with water,
Decocted, to obtain bletilla striata Aqueous extracts；And (2) mix obtained bletilla striata Aqueous extracts with ethanol, it is hereby achieved that
Precipitation containing Bletilla glucomannan.Inventor has found that this method is handled bletilla striata medicinal material by using the method that water extracts so that
Extraction process safety, no solvent residue, and compared with organic solvent, water resource is cheap and easy to get, while this method is simple, no
Special installation is needed, is adapted to factory, individual workship, Laboratory Production, carrying for Bletilla glucomannan can be significantly improved using this method in addition
Take efficiency.
According to an embodiment of the invention, in step (1), the mixed proportion of bletilla striata medicinal material and water is not particularly restricted,
According to a particular embodiment of the invention, the weight of bletilla striata medicinal material and water ratio can be 1:10~20.Inventor has found that the bletilla striata contains
More colloid polysaccharide, if solvent is very few so that the polysaccharide concentration of first dissolution is high, so as to prevent the further molten of bletilla polysaccharide
Go out, cause extraction incomplete, and solvent is too high, then extraction efficiency is too low, while adds the subsequent concentration time.For example, the bletilla striata
The weight of medicinal material and water ratio can be 1:15, thus, it is possible to further improve the yield of Bletilla glucomannan.
According to an embodiment of the invention, in step (1), the condition of decoction is not particularly restricted, according to the present invention's
It specific embodiment, can repeatedly be decocted, 2 hours every time, according to the specific example of the present invention, can be decocted three times,
2 hours every time.Inventor has found, number and decocting time is decocted by increasing, although bletilla striata glue yield and the bletilla striata can be improved
Polyoses content, but its amplification is smaller, and with the extension for decocting number and decocting time, decocting cost dramatically increases, therefore, comprehensive
Close and consider, decocted three times, can not only obtain within 2 hours higher bletilla striata glue yield and bletilla polysaccharide content every time, and
The energy can be saved.
According to an embodiment of the invention, in step (2), after bletilla striata Aqueous extracts are mixed with ethanol, the final concentration of ethanol
It is not particularly restricted, according to a particular embodiment of the invention, the final concentration of ethanol can be 55~80 volume %.Invention human hair
Existing, if concentration of alcohol is too low, bletilla polysaccharide precipitation is incomplete, causes damage；And concentration of alcohol, when reaching 80 volume %, polysaccharide is
It is complete through precipitating.
According to an embodiment of the invention,, in advance will be white before bletilla striata Aqueous extracts are mixed with ethanol in step (2)
Splendid achnatherum Aqueous extracts are concentrated.According to a particular embodiment of the invention, can be according to 3~7:1 ratio is concentrated.Invention human hair
It is existing, if being less than 3 according to cocnentration factor:1 is concentrated so that the dosage of subsequent ethanol is larger, and follow-up work amount is also larger, and concentrates
Than higher than 7:1, gained concentration fluid viscosity is larger, and block insoluble along with having, it is impossible to is normally carried out follow-up alcohol precipitation.According to this hair
Bright specific example, can be according to 5:1 ratio is concentrated.Thus, it is possible to further improve the extraction efficiency of Bletilla glucomannan.
According to an embodiment of the invention, in step (2), after bletilla striata Aqueous extracts are mixed with ethanol, the final concentration of ethanol
It can be 70 volume %, be filtered after standing overnight, filter residue is dried under reduced pressure to constant weight at 80 degrees celsius.Thus,
Bletilla striata glue yield and bletilla polysaccharide content can further be improved.
According to an embodiment of the invention, can the charred RADIX ET RHIZOMA RHEI containing 12~20 parts by weight and 30~50 weights in pharmaceutical composition
Measure the bletilla striata sol solution of part.Inventor has found that the main active principle of this medicine is micron rhubarb charcoal, and bletilla striata sol solution is used as this
The adjuvant of medicine, and excipient, if charred RADIX ET RHIZOMA RHEI ratio is too small, do not play therapeutic effect, and too high levels, cause medicine
Mobility is poor, it is also difficult to for used in clinic.
According to an embodiment of the invention, in pharmaceutical composition, the granularity of charred RADIX ET RHIZOMA RHEI is not particularly restricted, according to the present invention
Specific embodiment, the granularity of charred RADIX ET RHIZOMA RHEI can be 15~45 μm.Inventor has found, if charred RADIX ET RHIZOMA RHEI granularity is excessive, gained medicine
Settle too fast so that drug distribution is uneven, causes clinical administration dosage to be forbidden；And undersized, crushing difficulty is big, cost
Height, thus, clinical therapeutic efficacy needs are met using particle size range of the present invention, and meet modern pharmaceutical level.
According to an embodiment of the invention, in pharmaceutical composition, the concentration of bletilla striata sol solution is not particularly restricted, according to this
The specific embodiment of invention, the concentration of bletilla striata sol solution can be 0.1~0.2g/g.Inventor has found, if bletilla sol solution is dense
Spend height, Bletilla glucomannan is difficult to dissolve, and is also difficult to mix with charred RADIX ET RHIZOMA RHEI, and concentration is too low so that intermediate dose volume compared with
Greatly, troublesome in poeration, workload is big.
According to an embodiment of the invention, in pharmaceutical composition, the weight ratio of charred RADIX ET RHIZOMA RHEI and bletilla striata crude drug in bletilla striata sol solution
Can be 1:2.Thus, it is possible to significantly improve Hemostatics drug effect.
In another aspect of the present invention, the present invention proposes a kind of use of aforementioned pharmaceutical compositions in medicine is prepared
On the way.According to an embodiment of the invention, the medicine can be used for stopping blooding.According to a particular embodiment of the invention, the medicine can be used
In treatment Peptic Ulcer, specifically, by Forrest partings category Ia, Ib person.
Below with reference to specific embodiment, present invention is described, it is necessary to which explanation, these embodiments are only to describe
Property, without limiting the invention in any way.
Experimental example
1st, the extraction process by water of the preferred bletilla striata medicinal material of orthogonal test
Using Orthogonal Experiment and Design to bletilla striata glue yield, the influence factor of bletilla polysaccharide content, by amount of water, decoct when
Between, decoct number be used as investigation factor, tested from L9 (34) orthogonal arrage, gauge outfit design be shown in Table 1, and combine produce
Cost, energy etc. are considered.
The orthogonal array of table 1
1.1 test method
20g bletilla striata medicinal materials are weighed respectively, carry out decoction extraction according to table 1, filtrate is concentrated into medicinal material through 4 layers of filtered through gauze
5 times of amounts, stand to room temperature, add ethanol precipitation alcohol content is stood overnight up to 75 volume %, suction filtration is precipitated, and adds ethanol
Washing 3 times, filter to dry, be dried under reduced pressure (80 DEG C) to constant weight, produce Bletilla glucomannan finished product, it is standby.
1.2 evaluation index
1.2.1 the calculating of bletilla polysaccharide content：Using the content of Phenol-sulphate acid method measure bletilla polysaccharide.
1.2.2 the calculating of bletilla striata glue yield：Bletilla striata glue yield=Bletilla glucomannan weight/bletilla striata medicinal material weight × 100%
The measure of 1.3 bletilla polysaccharide contents
1.3.1 the preparation of reference substance solution
Precision weighs to be dried under reduced pressure to the DEXTROSE ANHYDROUS reference substance 55.10mg of constant weight through phosphorus pentoxide, puts 50ml appearances
In measuring bottle, it is dissolved in water and is diluted to scale, shake up and produce reference substance storing solution；
Precision draws storing solution 2.5ml, 3.5ml, 4.5ml, 5ml, 5.5ml into 100ml volumetric flasks, adds water to scale,
Shake up and produce control series product solution.
1.3.2 the preparation of need testing solution
Precision weighs Bletilla glucomannan 4mg, adds a small amount of water to heat, is stirred to dissolve, is transferred in 100ml volumetric flasks, add water-soluble
Solve and be diluted to scale, shake up and produce.
1.3.3 the selection of wavelength is determined
Precision draw 2ml water be used as blank solution, each 2ml of reference substance solution, need testing solution in drying tool plug test tube in,
The 4wt% phenol solution 1ml that each accurate addition newly configures, shake up, and rapid accurate addition sulfuric acid 7ml, shake up, then in 40 DEG C of water
30min is incubated in bath, takes out, puts 5min in ice-water bath, is taken out, according to 2010 editions Chinese Pharmacopoeia AASs, in 400~
500nm is scanned, and being as a result shown at 490nm has absorption maximum, and blank reagent is noiseless.
1.3.4 the investigation of linear relationship
Precision draws above-mentioned each 2ml of control series product solution, respectively into tool plug test tube, by being operated under 1.3.3 items, with water
For blank, absorbance is determined at 490nm wavelength, using absorbance as ordinate, concentration is that abscissa draws standard curve, is calculated
Obtaining regression equation is：Y=11.54C+0.013 (r=0.9947), shows reference substance solution in 27.55~60.61mgL-1Line
Sexual intercourse is good.
1.3.5 Precision Experiment
Precision draws reference substance solution 2ml, by being operated under 1.3.3 items, 5 absorbances of METHOD FOR CONTINUOUS DETERMINATION at 490nm wavelength,
Absorbance is respectively 0.453,0.448,0.454,0.439,0.440 (RSD=0.26%).
1.3.6 stability test
Precision draws need testing solution 2ml, and by being operated under 1.3.3 items, 1 suction is determined every 30min at 490nm wavelength
The RSD=1.07% of luminosity, as a result 2.5h internal absorbances, the results showed that need testing solution is stable in 2.5h.
1.3.7 replica test
Precision weighs No. 9 sample 4.2mg, 6 parts of parallel processing, by being operated under 1.3.3 items, determines and inhales at 490nm wavelength
Luminosity, the content of polysaccharide is calculated, the content average value of polysaccharide is 15.06%, RSD=0.66%, the results showed that the repetition of method
Property is good.
1.3.8 average recovery is tested
Precision weighs No. 9 sample 1.63mg, and precision adds reference substance solution 50ml (concentration 0.03306mg/ml), parallel
6 parts of processing, by being operated under 1.3.3, absorbance is determined at 490nm wavelength, the content of polysaccharide is calculated, the results are shown in Table 2.
The average recovery result of the test of table 2
1.3.9 sample size determines
Precision draws each need testing solution 2ml, into tool plug test tube, by being operated under 1.3.3 items, is surveyed at 490nm wavelength
Determine trap, per test sample parallel determination 3 times, calculate polyoses content by external standard method, the results are shown in Table 3, variance analysis is shown in Table 4.
The measure of 1.4 bletilla striata glue yields
Accurately weighed each gained Bletilla glucomannan weight, is calculated by bletilla striata glue yield formula, the results are shown in Table 3, variance analysis
It is shown in Table 4.
The L9 of table 3 (34) Orthogonal Experiment and Designs and measurement result
Note：A is bletilla striata glue yield；B is bletilla polysaccharide content
The variance analysis of table 4
Analyzed from table 3, the size that each factor influences on bletilla striata glue yield is respectively：C ＞ A ＞ B, contain to bletilla polysaccharide
Amount influence size be respectively：C ＞ B ＞ A；The results of analysis of variance of table 4 shows, decocts number to bletilla striata glue yield and bletilla polysaccharide
Content influences have significant, and decocting time influences have significant to bletilla striata polyoses content；By analyze judge and
From the point of view of saving the energy, the optimum extraction process for determining the bletilla striata is A2B3C3, i.e., bletilla striata medicinal material adds 15 times of amount water extractions 3
It is secondary, 2 hours every time.
1.5 checking test
Bletilla striata medicinal material 20g is weighed respectively, and according to the optimised process obtained by orthogonal experiment, (i.e. bletilla striata medicinal material adds 15 times of amount water
Extraction 3 times, every time 2 hours) verified, as a result bletilla striata glue yield is 19.61%, and bletilla polysaccharide content is 15.97%, is passed through
Comparing proves that the technique is defined as bletilla striata water extraction optimised process.
2nd, the process conditions selection of alcohol precipitation
The investigation of 2.1 Aqueous extracts concentrating degrees
Bletilla striata medicinal material 20g is taken, is extracted according to the optimised process obtained by orthogonal experiment, gained extract solution is dense by table 5
Shorten different degrees of concentrate into, cool down, add ethanol, be allowed to alcohol content in precipitation solution and, up to 75%, be stored at room temperature overnight, take out
Filter, 80 DEG C are dried under reduced pressure to constant weight, weighed weight, observe Bletilla glucomannan color, and calculate bletilla striata glue yield and bletilla polysaccharide contains
Amount, the results are shown in Table shown in 5.
Influence of the bletilla striata Aqueous extracts concentrating degree of table 5 to Bletilla glucomannan color, yield and bletilla polysaccharide content
As shown in Table 5, concentrating degree 5:Concentrate outward appearance when 1, bletilla striata glue yield and bletilla polysaccharide content and 7:When 1
It is close, it is contemplated that 7 during alcohol precipitation:1 concentrate is especially sticky and has block insoluble matter, therefore it is 5 to select concentrating degree:1.
The investigation of 2.2 alcohol precipitation concentrations
It is 5 to select concentration:1 concentrate, different amounts of ethanol is added, it is quiet to be allowed to alcohol content difference, room temperature in precipitation solution
Put overnight, filter, 80 DEG C are dried under reduced pressure to constant weight, weighed weight, observe Bletilla glucomannan outward appearance, and calculate bletilla striata glue yield and white
Splendid achnatherum polyoses content, as a result it see the table below shown in 6.
Influence of the bletilla striata water extracting liquid alcohol precipitation concentration of table 6 to Bletilla glucomannan outward appearance, yield and bletilla polysaccharide content
As shown in Table 6, when alcohol content is 70 volume %, gained Bletilla glucomannan outward appearance, bletilla striata glue yield and polyoses content and
The volume % of alcohol content 75,80 volume % are close, it is contemplated that save ethanol cost, therefore it is 70 volume % to select alcohol precipitation concentration.
2.3 checking test
It is 1 to select three parts of concentration:5 bletilla striata concentrate, ethanol is added, it is 70 volume % to be allowed to alcohol content in precipitation solution,
It is stored at room temperature overnight, filters, 80 DEG C is dried under reduced pressure to constant weight, weighed weight, and observation Bletilla glucomannan outward appearance is beige loose
Fritter, bletilla striata glue yield are 19.60%, and bletilla polysaccharide content is 15.91%, and it is alcohol precipitation optimised process as a result to prove the technique.
3rd, medicine Physical Experiment
According to preferable extraction process (i.e. bletilla striata medicinal material adds 15 times of amount water and extracted 3 times, every time 2 hours), the bletilla striata is weighed
100.0g, add water that 0.2g/g bletilla striata sol solution is made, it is standby.According to formula rate, the charred RADIX ET RHIZOMA RHEI bletilla striata of different proportion is prepared
Sol solution.
3.1 experimental procedure
Bletilla striata medicinal material is soaked into 30min, then plus 15 times of amount water extract 3 times, each 2h, merge extract solution, concentration
It is standby to about 40mL；Charred RADIX ET RHIZOMA RHEI accurately is weighed, is slowly added under stirring in bletilla striata extract, sodium benzoate preservative is added, adds
Water stirs, produced to 100g.
3.2 method of testing
Fluidity testing：Take final drug to be placed in beaker, stirred with glass bar, feel the resistance of glass bar, from beaker
Another beaker is poured into, is detected by an unaided eye.
Stomach tube is tested：The medicine stirred evenly is extracted with 50mL syringes, stomach tube is inserted, with manual injection device, sees that can medicine
Pass through stomach tube, and difficulty or ease, drag size；After having pushed away, pipeline whether can clear water cleaning it is unobstructed.
3.3 test results are as shown in table 7.
The charred RADIX ET RHIZOMA RHEI of table 7 and Bletilla glucomannan different proportion proportioning
Conclusion：Although as can be seen that 4, No. 5 doses are larger, resistance is larger for experiment more than, being not suitable for clinic should
With；1st, 2, No. 3 clinics can be applied, but No. 3 single dose of drug are bigger than 1, No. 2, wish that unit dose is larger with reference to clinic
It is required that determine that technique is No. 3：That is 16% charred RADIX ET RHIZOMA RHEI, 8% bletilla striata water (40g, 0.2g/g) extract is added.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or the spy for combining the embodiment or example description
Point is contained at least one embodiment or example of the present invention.In this manual, to the schematic representation of above-mentioned term not
Identical embodiment or example must be directed to.Moreover, specific features, structure, material or the feature of description can be with office
Combined in an appropriate manner in one or more embodiments or example.In addition, in the case of not conflicting, the skill of this area
Art personnel can be tied the different embodiments or example and the feature of different embodiments or example described in this specification
Close and combine.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changed, replacing and modification.
Claims (6)
- A kind of 1. pharmaceutical composition for being used to stopping blooding or treating Peptic Ulcer, it is characterised in that the medicine group The active principle of compound consists of the following composition：The charred RADIX ET RHIZOMA RHEI of 16 parts by weight, the granularity of the charred RADIX ET RHIZOMA RHEI is 15~45 μm；AndThe bletilla striata sol solution of 40 parts by weight, the concentration of the bletilla striata sol solution is 0.2g/g.
- 2. pharmaceutical composition according to claim 1, it is characterised in that described based on 100 grams of described pharmaceutical composition Pharmaceutical composition consists of the following composition：16 grams of charred RADIX ET RHIZOMA RHEIs, 40 grams of bletilla striata sol solutions, 0.5 gram of sodium benzoate preservative, 43.5 grams of water.
- 3. pharmaceutical composition according to claim 1, it is characterised in that the Bletilla glucomannan is to obtain through the following steps 's：(1) bletilla striata medicinal material is mixed with water, decocted, to obtain bletilla striata Aqueous extracts；And(2) bletilla striata Aqueous extracts are mixed with ethanol, to obtain the precipitation containing Bletilla glucomannan,Wherein, in step (1), the weight ratio of the bletilla striata medicinal material and the water is 1:15,In step (1), decocted three times, 2 hours every time,In step (2), after the bletilla striata Aqueous extracts are mixed with the ethanol, the final concentration of 70 volume % of ethanol,It is in advance that the bletilla striata Aqueous extracts are dense before the bletilla striata Aqueous extracts are mixed with the ethanol in step (2) Contracting, wherein, according to 5:1 ratio carries out the concentration,In step (2), after the bletilla striata Aqueous extracts are mixed with the ethanol, the final concentration of 70 volume % of ethanol, stand Filtered after overnight, filter residue is dried under reduced pressure to constant weight at 80 degrees celsius.
- A kind of 4. method for preparing the pharmaceutical composition for stopping blooding or treating Peptic Ulcer, it is characterised in that Including：Bletilla glucomannan is obtained through the following steps：(1) bletilla striata medicinal material is mixed with water, decocted, to obtain bletilla striata Aqueous extracts； And (2) mix the bletilla striata Aqueous extracts with ethanol, to obtain the precipitation containing Bletilla glucomannan, wherein, in step (1), The weight ratio of the bletilla striata medicinal material and the water is 1:15, in step (1), decocted three times, 2 hours every time, in step (2) in, after the bletilla striata Aqueous extracts are mixed with the ethanol, the final concentration of 70 volume % of ethanol, in step (2), Before the bletilla striata Aqueous extracts are mixed with the ethanol, the bletilla striata Aqueous extracts are concentrated in advance, wherein, according to 5:1 ratio Example carries out the concentration, in step (2), after the bletilla striata Aqueous extracts are mixed with the ethanol, and final concentration of the 70 of ethanol Volume %, filtered after standing overnight, filter residue is dried under reduced pressure to constant weight at 80 degrees celsius；The Bletilla glucomannan is configured to the bletilla striata sol solution that concentration is 0.2g/g；The bletilla striata sol solution is mixed with charred RADIX ET RHIZOMA RHEI, sodium benzoate preservative and water, wherein, based on 100 grams of the medicine Composition, described pharmaceutical composition include 16 grams of charred RADIX ET RHIZOMA RHEIs, 40 grams of bletilla striata sol solutions, 0.5 gram of sodium benzoate preservative, 43.5 grams Water, the concentration of the bletilla striata sol solution is 0.2g/g.
- 5. purposes of the pharmaceutical composition described in any one of claims 1 to 3 in medicine is prepared, the medicine is used to stop blooding.
- 6. purposes according to claim 5, the medicine is used to treat Peptic Ulcer.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1042079A (en) * | 1988-10-20 | 1990-05-16 | 南京市鱼品加工厂 | A kind of method and special equipment thereof of producing Pseudobulbus Bletillae (Rhizoma Bletillae) glue |
| CN102380039A (en) * | 2010-09-01 | 2012-03-21 | 董玺 | Oral traditional Chinese medicine prescription for treating upper gastrointestinal hemorrhage |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100962587B1 (en) * | 2007-10-02 | 2010-06-11 | 동국대학교 산학협력단 | A method for fermentation of natural plants and herbal medicines, a fermented product prepared therefrom and a paharmaceutical composition, a cosmetic compositon and a food composition comprising the product |
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2015
- 2015-01-22 CN CN201510032265.4A patent/CN104689065B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1042079A (en) * | 1988-10-20 | 1990-05-16 | 南京市鱼品加工厂 | A kind of method and special equipment thereof of producing Pseudobulbus Bletillae (Rhizoma Bletillae) glue |
| CN102380039A (en) * | 2010-09-01 | 2012-03-21 | 董玺 | Oral traditional Chinese medicine prescription for treating upper gastrointestinal hemorrhage |
Non-Patent Citations (3)
| Title |
|---|
| 微米大黄炭白芨胶对乙醇源性急性糜烂性出血性胃炎影响的动物实验及临床研究;杨帆;《中国优秀硕士学位论文全文数据库医药卫生科技辑》;20141115(第11期);第7页5结果与分析 * |
| 微米大黄炭白芨胶对模型大鼠急性胃粘膜病变修复研究;杨帆等;《湖北中医杂志》;20140228;第36卷(第02期);第21页左栏第1-9行，第22页右栏第14-19行 * |
| 正交试验优选白及粗多糖的提取工艺;韩涛等;《第二届中药现代化新剂型新技术国际学术会议》;20061231;第493页第5-6行，第494页倒数第1-3行，第496页第3-12页 * |
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| CN104689065A (en) | 2015-06-10 |
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