CN104686512A - Application of ISR mechanism based disease-resistant inductive agent in preventing and treating turfgrass disease - Google Patents
Application of ISR mechanism based disease-resistant inductive agent in preventing and treating turfgrass disease Download PDFInfo
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- CN104686512A CN104686512A CN201410717486.0A CN201410717486A CN104686512A CN 104686512 A CN104686512 A CN 104686512A CN 201410717486 A CN201410717486 A CN 201410717486A CN 104686512 A CN104686512 A CN 104686512A
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Abstract
The invention discloses application of an ISR mechanism based disease-resistant inductive agent in preventing and treating turfgrass disease. The inductive agent is a 2, 3-butanediol stereisomer mixture, and can induce the original herbal disease resistance of turfgrass, and has obvious preventing and treating effects on agrostis stolonifera, meadow grass, brown blotch of perennial ryegrass and festuca arundinacea and fusarium blight by applying to the root part with the most proper concentrations (250, 550, 450 and 250 mu mol/L), and the effect of resisting brown blotch can respectively reach 86, 68.5, 73.2 and 81%. When the inductive agent is affected to agrostis stolonifera, the activities of phenylalnine ammonialyase, chalcone isomerase and 4-coumarate coenzyme A ligase are all improved obviously. The inductive agent can obviously increase the content of secondary metabolism products relevant to the agrostis stolonifera resistance, such as total hydroxybenzene, flavones, lignin, can be applied to the prevention and treatment of turfgrass disease, can reduce the use of chemical bactericides, protect the environment, and reduce the turfgrass maintenance expense.
Description
Technical field
The present invention relates to a kind of application of plant disease resistance inductor in Diseases of Turfgrass control based on ISR mechanism.
Background technology
Lawn is the important component part of urban afforestation, and in recent years along with the raising of living standards of the people and the enhancing of environmental consciousness, China's Development of Turf is swift and violent.Along with a large amount of introductions of external grass seeds, the continuous expansion of greenery area and lawn intensive management degree improve, Diseases of Turfgrass becomes affects lawn value, one of the principal element containing current Development of Turf.In Turfgrass Growth process, be subject to infecting of multiple pathogen, make lawn occur variable color, withered, wilt, the symptom such as to rot, thus have a strong impact on overall ornamental value, the use value on lawn, reduce turf quality and useful life.
Common Diseases of Turfgrass, if brown spot is one of the widest disease that distributes in all Diseases of Turfgrass, can infect all known turfgrasss, when soil temperature rises to 15-20 DEG C, pathogen just can produce a large amount of mycelia, under hot and humid condition, the rapid large area morbidity in lawn; Fusarium wilt is the second largest serious plant disease being only second to brown spot in China, and high temperature and drought easily causes root, stem rotten, and the hot and humid large area of tikka that easily causes occurs.A generation for lawn disease, can cause local, lawn or the death of most of area, until whole lawn goes to wreck.By traditional disease control way, as selected disease-resistant variety, reasonable management and using the effect of chemical bactericide control Diseases of Turfgrass very limited.Tradition disease resistant and breeding method breeding cycle is long, and parent material obtains limited, only has certain prevention effect to disease.Reduce irrigation frequency, suitably fertilising to improve plant vigor, control the control measures such as cutting range and frequency, only reduce disease to a certain extent and occur, reduce disease generation severity.Chemical control effect is rapid, Be very effective, using method are easy to grasp, be the important means of control Diseases of Turfgrass, but chemical control cost is higher, pathogen easily produces drug resistance even pesticide resistance to bactericide, facts have proved, chemical bactericide is scarcely desirable to the preventive effect of disease.Particularly one carry, after chemical bactericide sprays in a large number in soil accumulation serious environment pollution, and then affect the health of the mankind.Along with molecular biological development and to plant with pathogen is interactional understands in depth, utilize derivant inducing plant basis disease resistance proposed and apply, this theory and technology has resistance, the advantage such as lasting, free from environmental pollution.
Inducing plant disease resistance utilizes physics, chemistry and biological method, anticipates plant, changes disease reaction, makes original susceptible position produce the disease resistance phenomenon of local or system.Scientific research is developed so far, and causes the systematicness mechanism luring anti-reflective to answer to illustrate not yet completely, the mechanism of concerned plant Anti-different region antibodies, generally admits two kinds of modes, i.e. systemic acquired resistance (SAR) and inducible system resistance (ISR) at present.A kind of systemic resistance can be inspired in plant corpus after harmful pathogen primary infection, plant corpus is not infected position and is produced resistance of wide spectrum to pathogenic bacteria (virus, fungi, bacterium) yet, this disease resistance needs salicylic acid signal transduction path to mediate, and namely claims systemic acquired resistance (SAR); Carried out large quantity research to the systemic acquired resistance (SAR) of plant, BTH, salicylic acid, methyl jasmonic acid, Benzothiadiazole, oxalic acid etc. all can be used as SAR derivant.SAR derivant is for preventing and treating in the research of Diseases of Turfgrass, and Lee etc. report that BTH can induce creeping bentgrass Crenshaw, Penncross, Providence kind to produce disease resistance, but are invalid at other kind such as L-93, BTH.
Inducible system resistance (ISR) is then activated by the non-pathogenic plant growth-promoting rhizobacteria being distributed in plant corpus root, relies on jasmonic/Ethylene Signal Transduction approach and makes plant body obtain disease resistance.Produce in ISR process plant, some plant growth-promoting rhizobacteria analogs are by starting the disease resistance mechanisms in plant corpus, reduce the incidence of disease of above-ground plant parts, deeply specifically studying of derivant relevant to ISR is very limited, about the turfgrass Anti-different region antibodies research report based on ISR mode is very few especially.
Suzuki in 2003 etc. find that P.fluorescens strain HP72 can cause the resistance of creeping bentgrass Rhizoctonia solani (R.solani) in the Anti-different region antibodies research of creeping bentgrass.The research such as Ryu in 2004 is thought, the volatile organic matter 2,3-butanediol of plant growth-promoting rhizobacteria secretion and isomer thereof produce in the process of disease resistance at inducing plant has important effect.2,3-butanediol (CH3CHOHCHOHCH3,2,3-Butanediol) there are 3 kinds of optical isomers, be respectively D-(-)-2,3-butanediol, L-(+)-2,3-butanediol and meso-2,3-butanediol are a kind of transparency liquid of colorless and odorless under normal temperature.2010, Canada's scholar Cortes-Barco research finds, 2, levo form (the 2R of 3-butanediol, 3R-butanediol) suppress by fungi Microdochiumnivale, 3 kinds of lawn leaf diseases that Rhizoctoniasolani, Sclerotiniahomoeocarpa cause, can reduce and reduce creeping bentgrass leaf disease region and reach 20%-40%.2011, the levo form of the auspicious report 2,3-butanediol of horse can induce creeping bentgrass Resistance to Cercospora, and to connect after bacterium the 15th day, disease index is down to 31.33% by contrast 93.33%, and disease resisting effect is 66.4%.
Summary of the invention
The key issue that the present invention will solve is the defect overcoming existing Diseases of Turfgrass prevention and controls, provides a kind of lawn of can effectively inducing and produces broad spectrum resistance and the Diseases of Turfgrass Prevention Technique (theory) of environmental protection and energy saving.Make its to creeping bentgrass, kentucky blue grass, English ryegrass etc. for the turfgrass typical disease of representative has obvious preventive and therapeutic effect.
The technical problem to be solved in the present invention is to provide a kind of efficient inducer based on ISR mechanism---and 2,3-butanediol (2, the mixture of 3-butanediol stereoisomer), to induce turfgrass to produce disease resistance, show significant Anti-different region antibodies effect from aspects such as phenotypic characteristic, the response of phenylpropyl alcohol alkane metabolic defenses enzyme and disease-resistant relevant secondary metabolites changes of contents thereof.There is provided the application method of above-mentioned derivant, the activity that namely this Plant elicitors is suitable and the mode of action simultaneously.
To achieve these goals, technical scheme provided by the invention is: a kind of inducer based on ISR mechanism produces the application in disease resistance at induction turfgrass, described derivant is 2,3-butanediol stereoisomer mixture, structure such as formula shown in (I),
Formula (I).
Further, described turfgrass is creeping bentgrass, kentucky blue grass, English ryegrass or Festuca Arundinacea.
Further, the method that applies of described derivant is that root applies method, and induction turfgrass produces disease resistance.
Further, above-mentioned application, the concentration of described derivant induction creeping bentgrass Resistance to Cercospora is 250-350 μm of ol/L; The concentration of described derivant induction kentucky blue grass Resistance to Cercospora is 550 μm of ol/L; The concentration of described derivant induction English ryegrass Resistance to Cercospora is 350-450 μm of ol/L; The concentration of described derivant induction Festuca Arundinacea Resistance to Cercospora is 250-350 μm of ol/L.
Further, above-mentioned application, the preferred concentration of described derivant induction creeping bentgrass Resistance to Cercospora is 250 μm of ol/L; The preferred concentration of described derivant induction English ryegrass Resistance to Cercospora is 450 μm of ol/L; The preferred concentration of described derivant induction Festuca Arundinacea Resistance to Cercospora is 250 μm of ol/L.
Further, the concentration of the anti-fusarium wilt of described derivant induction creeping bentgrass is 250-350 μm of ol/L.
Further, the preferred concentration of the anti-fusarium wilt of described derivant induction creeping bentgrass is 250 μm of ol/L.
Above-mentioned derivant is to the prophylactico-therapeutic measures of Diseases of Turfgrass and effect analysis thereof:
1, the main component of described derivant is 2,3-butanediol stereoisomer mixture:
2,3-butanediol (structural formula is shown in following formula I for the mixture of 2,3-butanediol stereoisomer, CAS No:513-85-9) can effectively induce lawn plant disease resistance to strengthen.
I (stereoisomer mixture)
2, described plant is turfgrass:
Be preferably creeping bentgrass, kentucky blue grass, English ryegrass.
3, described Turfgrass Diseases is brown spot or fusarium wilt:
4, activity and the phenotypic resistance inducing effect thereof of anti-method is lured described in:
1) provided by the invention 2, the method of 3-butanediol stereoisomer mixture induction creeping bentgrass Resistance to Cercospora, fusarium wilt, wherein, 2, the concentration of 3-butanediol stereoisomer mixture is 250-350 μm of ol/L, is preferably 250 μm of ol/L, within after this concentration derivant process the 7th day, inoculates Rhizoctonia solani Kuhn, Fusarium oxysporum, to connect after bacterium the 15th day, can 86.08% be reached to the control efficiency of brown spot; To connect after bacterium the 21st day, can 78.26% be reached to the control efficiency of fusarium wilt;
2) provided by the invention 2, the method of 3-butanediol stereoisomer mixture induction kentucky blue grass Resistance to Cercospora, wherein, 2, the concentration of 3-butanediol stereoisomer mixture is 550 μm of ol/L, 7th day inoculation Rhizoctonia solani Kuhn after derivant process, to connect after bacterium the 30th day, control efficiency can reach 68.5%;
3) provided by the invention 2, the method of 3-butanediol stereoisomer mixture induction English ryegrass Resistance to Cercospora, wherein, 2, the concentration of 3-butanediol stereoisomer mixture is 350-450 μm of ol/L, is preferably 450 μm of ol/L, the 7th day inoculation Rhizoctonia solani Kuhn after this concentration derivant process, to connect after bacterium the 45th day, control efficiency can reach 73.2%;
4) provided by the invention 2, the method of 3-butanediol stereoisomer mixture induction Festuca Arundinacea Resistance to Cercospora, wherein, 2, the concentration of 3-butanediol stereoisomer mixture is 250-350 μm of ol/L, is preferably 250 μm of ol/L, the 7th day inoculation Rhizoctonia solani Kuhn after this concentration derivant process, to connect after bacterium the 45th day, control efficiency can reach 81%.
5, described derivant acts on the response of plant corpus phenylpropyl alcohol alkane metabolic defenses enzyme after creeping bentgrass:
After the 2,3-butanediol stereoisomer mixture of 250 μm of ol/L, water (contrast) process creeping bentgrass 7 days, inoculation Rhizoctonia solani Kuhn.In the creeping bentgrass blade of derivant process after inoculation, phenylalnine ammonialyase (PAL), enzyme, namely chalcone isomerase (CHI), 4-coumarate CoA-ligase (4CL) activity all significantly improve compared with the control.
6, after described derivant acts on creeping bentgrass, in phenylpropyl alcohol alkane metabolic pathway, the relevant secondary metabolites of defence changes:
After the 2,3-butanediol stereoisomer mixture of 250 μm of ol/L, water (contrast) process creeping bentgrass 7 days, inoculation Rhizoctonia solani Kuhn.2, the mixture of 3-butanediol stereoisomer can significantly improve the disease-resistant relevant secondary metabolites of creeping bentgrass phenylpropyl alcohol alkane metabolic pathway-total phenol, flavones and content of lignin, wherein, to connect after bacterium the 5th day, total phenol content reaches 1.087mg/ (gFW); Latter 9th day of inoculation, flavones content reaches 0.027mg/ (gFW); To connect after bacterium the 11st day, content of lignin reaches 0.599 OD
280g
-1fW.
7, the mode of action of anti-method is lured described in:
Preferably, adopt the method that root applies, it is not obvious that leaf portion sprays disease resisting effect.
Beneficial effect of the present invention is:
The invention provides a kind of derivant-2 of inducing turfgrass to produce broad spectrum resistance, the mixture of 3-butanediol stereoisomer, and suitable application concentration and application mode, it can induce multiple turfgrass to produce resistance to several typical disease effectively, additionally provides the case evidence that derivant is applied in creeping bentgrass brown spot, fusarium wilt and kentucky blue grass, English ryegrass and Festuca Arundinacea Control of Lecanosticta Acicola.
Derivant produces ISR as signaling molecule inducing plant, strengthens plant self resistance thus resist infecting of pathogen.Plant elicitors of the present invention is 2, the mixture of 3-butanediol stereoisomer, with levo form 2R, 3R-butanediol is all disease resistance of plant derivant, but compared with its levo form, the control of this derivant to disease is more effective, duration obviously extends, and the mixture of isomer does not need again to be separated in production technology, more easily obtains, practical more economically.Derivant 2 provided by the invention, 3-butanediol stereoisomer mixture, within 15 days after connecing bacterium, can 86.08% be reached to the preventive effect of creeping bentgrass brown spot, more 30-45 days can be extended to effective control phase of kentucky blue grass, English ryegrass and Festuca Arundinacea brown spot, all obviously are better than control efficiency and effective acting time the levo form of 2,3-butanediol.
2, the mixture of 3-butanediol stereoisomer does not have direct inhibitory action to pathogen, but there is the resistance of obvious inducing plant self thus reach disease-resistant effect, be particularly suitable for root to use, root is used, impact by environmental factor is little, can effectively induce turfgrass to produce resistance to multiple diseases.Use turfgrass Plant elicitors control Diseases of Turfgrass; compensate for the weak point of traditional prophylactico-therapeutic measures; particular avoid chemical control cost higher; the shortcomings such as easy contaminated environment; this is one of the control of the current Diseases of Turfgrass effectively approach of environmental protection, can reduce the use of chemical bactericide, protection of the environment; save lawn maintenance expense, for new road is opened up on the fully natural green lawn creating pollution-free nonhazardous future.
Describedly lure anti-method advantage:
1,2,3-butanediol stereoisomer mixture all has significant Anti-different region antibodies effect in multiple turfgrass, can be widely used in Diseases of Turfgrass control;
2, derivant has resistance to multiple pathogen;
3, utilizing 2,3-butanediol stereoisomer mixture to prevent and treat Diseases of Turfgrass is a kind of biological control method efficiently, has resistance, the advantage such as lasting, free from environmental pollution compared with chemical pesticide;
4, derivant brings out the intrinsic disease resistance of turfgrass basis, and derivant self does not work to pathogen, and pathogen can not be made to develop immunity to drugs;
5, Plant elicitors is as signaling molecule, and only the composition of trace will induce the disease-resistant potentiality of turfgrass self, and its derivant itself can not cause poisoning to lawn, and reduces the cost of Diseases of Turfgrass control;
6, derivant is used by root, and the impact by environmental factor is little.
Accompanying drawing explanation
Fig. 1 is derivant process creeping bentgrass provided by the invention and inoculates the disease resisting effect figure of Rhizoctonia solani Kuhn after 15 days.
Wherein, left figure connects bacterium after the 2,3-butanediol process of 250 μm of ol/L, and middle figure does not directly connect bacterium with 2,3-butanediol process, and right figure does not connect bacterium with derivant process.
Fig. 2 is derivant process creeping bentgrass provided by the invention and inoculates the disease resisting effect figure of Fusarium oxysporum after 21 days.
Wherein, left figure connects bacterium after the 2,3-butanediol process of 250 μm of ol/L, and middle figure does not directly connect bacterium with 2,3-butanediol process, and right figure does not connect bacterium with derivant process.
Fig. 3 is derivant treating meadow annual bluegrass provided by the invention and inoculates the disease resisting effect figure of Rhizoctonia solani Kuhn after 30 days.
Wherein, left figure connects bacterium after the 2,3-butanediol process of 550 μm of ol/L, and middle figure does not directly connect bacterium with 2,3-butanediol process, and right figure does not connect bacterium with derivant process.
Fig. 4 is derivant process English ryegrass provided by the invention and inoculates the disease resisting effect figure of Rhizoctonia solani Kuhn after 45 days.
Wherein, left figure connects bacterium after the 2,3-butanediol process of 450 μm of ol/L, and middle figure does not directly connect bacterium with 2,3-butanediol process, and right figure does not connect bacterium with derivant process.
Fig. 5 is derivant process Festuca Arundinacea provided by the invention and inoculates the disease resisting effect of Rhizoctonia solani Kuhn after 45 days.
Wherein, left figure connects bacterium after the 2,3-butanediol process of 250 μm of ol/L, and right figure does not directly connect bacterium with 2,3-butanediol process.
Fig. 6 is the impacts of 250 μm of ol/L 2,3-BD on PAL activity in creeping bentgrass body.
Fig. 7 is the impacts of 250 μm of ol/L 2,3-BD on CHI activity in creeping bentgrass body.
Fig. 8 is the impacts of 250 μm of ol/L 2,3-BD on 4CL activity in creeping bentgrass body.
Fig. 9 is the impacts of 250 μm of ol/L 2,3-BD on total phenol content in creeping bentgrass body.
Figure 10 is the impacts of 250 μm of ol/L 2,3-BD on flavones content in creeping bentgrass body.
Figure 11 is the impacts of 250 μm of ol/L 2,3-BD on content of lignin in creeping bentgrass body.
Embodiment
Embodiment 1:
2,3-butanediol stereoisomer mixture used in the present invention is purchased from West Asia reagent, and No. CAS is 513-85-9, and molecular formula is C
4h
10o
2, molecular weight is 90.12.
The industrial process of 2,3-butanediol: the four carbon hydrocarbons produced by petroleum cracking are hydrolyzed and obtain under high temperature, high pressure.At present, produce 2,3-butanediol by fermentable and become its production source.Such as: with carbohydrate, molasses, mash or alcohol mother liquor etc. for raw material, obtain through biological fermentation process.When carbohydrate fermentation preparing alcohol and then vinegar processed, wherein by-product acetaldehyde also can generate 2,3-butanediol under saccharomycete effect.
Rhizoctonia solani Kuhn is inoculated, the analysis of its inducing effect after derivant process creeping bentgrass of the present invention:
Creeping bentgrass seed soaks 5h, 70% alcohol solution dipping 1min, and 10% clorox soaks 15min, and aseptic water washing 6-7 time, plants in 240ml tissue culture bottle, and cultivate in 25 DEG C of group training rooms, the photoperiod is 16h/d.Latter 8th day of plantation, the 2,3-butanediol of 150-550 μm of ol/L establishes 5 concentration gradients altogether, gets 10ml respectively and is injected to creeping bentgrass root, contrast injection same volume sterile water.After derivant process the 7th day, (concentration was OD to spray inoculation Rhizoctonia Solani suspension
380=0.8), every bottle of 3ml, 25 DEG C of cultivations, contrast spray same volume sterile water.Latter 15th day of inoculation, the successful of 2,3-butanediol stereoisomer mixture induction creeping bentgrass Resistance to Cercospora, wherein 250 μm of ol/L inducing effect can reach 86.08%, as shown in Figure 1, table 1 is the screening of 2,3-butanediol suitable concentration (after inoculation the 15th day) to inducing effect.
Table 1
| Contrast | 150μmol/L | 250μmol/L | 350μmol/L | 450μmol/L | 550μmol/L | |
| Disease index (%) | 91 | 68.67 | 12.67 | 45.52 | 78.67 | 90 |
| Disease resisting effect (%) | — | 24.54 | 86.08 | 49.98 | 13.55 | 1 |
Embodiment 2:
Fusarium oxysporum is inoculated, the analysis of its inducing effect after derivant process creeping bentgrass of the present invention:
Creeping bentgrass plantation as described in Example 1.Latter 8th day of plantation, the 2,3-butanediol of 150-550 μm of ol/L establishes 5 concentration gradients altogether, gets 10ml respectively and is injected to creeping bentgrass root, contrast injection same volume sterile water.After derivant process the 7th day, (concentration was 1 × 10 to spray inoculation Fusarium oxysporum spore suspension
6individual/ml), every bottle of 7ml, contrast spray same volume sterile water.28 DEG C of dark culturing 1 day after inoculation, cultivate the group training room of 26-27 DEG C afterwards.Latter 21st day of inoculation, the successful of 250 μm of anti-fusarium wilts of ol/L 2,3-butanediol stereoisomer mixtures induction creeping bentgrass, disease resisting effect can reach 78.26%, as shown in Figure 2, table 2 is the screening of 2,3-butanediol suitable concentration (after inoculation the 21st day) to inducing effect.
Table 2
| Contrast | 150μmol/L | 250μmol/L | 350μmol/L | 450μmol/L | 550μmol/L | |
| Disease index (%) | 92 | 13.04 | 20 | 40 | 62.6 | 84 |
| Disease resisting effect (%) | — | 16.3 | 78.26 | 56.52 | 31.96 | 8.7 |
Embodiment 3:
Rhizoctonia solani Kuhn is inoculated, the analysis of its inducing effect after derivant treating meadow annual bluegrass of the present invention:
Kentucky blue grass is planted in tissue culture bottle, after emerging the 20th day, and the 2,3-butanediol of 150-550 μm of ol/L establishes 5 concentration gradients altogether, gets 10ml respectively and is injected to kentucky blue grass root, contrast injection same volume sterile water.After derivant process the 7th day, (concentration was OD to spray inoculation Rhizoctonia Solani suspension
380=0.8), every bottle of 4ml, 25 DEG C of cultivations, contrast spray same volume sterile water.Latter 30th day of inoculation, the successful of the 2,3-butanediol stereoisomer mixture induction kentucky blue grass Resistance to Cercospora of 550 μm of ol/L, disease resisting effect can reach 68.5%, as shown in Figure 3, table 3 is the screening of 2,3-butanediol suitable concentration (after inoculation the 30th day) to inducing effect.
Table 3
| Contrast | 150μmol/L | 250μmol/L | 350μmol/L | 450μmol/L | 550μmol/L | |
| Disease index (%) | 100 | 100 | 97.6 | 100 | 86.67 | 31.5 |
| Disease resisting effect (%) | — | 0 | 2.4 | 0 | 13.33 | 68.5 |
Embodiment 4:
Rhizoctonia solani Kuhn is inoculated, the analysis of its inducing effect after derivant process English ryegrass of the present invention:
English ryegrass is planted in tissue culture bottle, emerges latter 20 days, and prune once, the 2,3-butanediol of 150-550 μm of ol/L establishes 5 concentration gradients altogether, gets 10ml respectively and is injected to English ryegrass root, contrast injection same volume sterile water.After derivant process the 7th day, (concentration was OD to spray inoculation Rhizoctonia Solani suspension
380=0.8), every bottle of 4ml, 25 DEG C of cultivations, contrast spray same volume sterile water.Latter 45th day of inoculation, the successful of the 2,3-butanediol stereoisomer mixture induction English ryegrass Resistance to Cercospora of 450 μm of ol/L, disease resisting effect can reach 73.2%, as shown in Figure 4, table 4 is the screening of 2,3-butanediol suitable concentration (after inoculation the 45th day) to inducing effect.
Table 4
| Contrast | 150μmol/L | 250μmol/L | 350μmol/L | 450μmol/L | 550μmol/L | |
| Disease index (%) | 100 | 87.6 | 79.4 | 33.4 | 26.8 | 74.8 |
| Disease resisting effect (%) | — | 12.4 | 20.6 | 66.6 | 73.2 | 25.2 |
Embodiment 5:
Rhizoctonia solani Kuhn is inoculated, the analysis of its inducing effect after derivant process Festuca Arundinacea of the present invention:
Festuca Arundinacea is planted in tissue culture bottle, emerges latter 15 days, and the 2,3-butanediol pruning twice, 150-550 μm of ol/L establishes 5 concentration gradients altogether, gets 10ml respectively and is injected to Festuca Arundinacea root, contrast injection same volume sterile water.After derivant process the 7th day, (concentration was OD to spray inoculation Rhizoctonia Solani suspension
380=0.8), every bottle of 7ml, cultivates at the natural environment of 28-35 DEG C, contrast spray same volume sterile water.Latter 45th day of inoculation, the successful of the 2,3-butanediol stereoisomer mixture induction Festuca Arundinacea Resistance to Cercospora of 250 μm of ol/L, disease resisting effect can reach 81%, as shown in Figure 5, table 5 is the screening of 2,3-butanediol suitable concentration (after inoculation the 45th day) to inducing effect.
Table 5
| Contrast | 150μmol/L | 250μmol/L | 350μmol/L | 450μmol/L | 550μmol/L | |
| Disease index (%) | 100 | 92.8 | 19 | 59 | 90.4 | 99 |
| Disease resisting effect (%) | — | 7.2 | 81 | 41 | 9.6 | 1 |
Embodiment 6:
Rhizoctonia solani Kuhn is inoculated, phenylpropyl alcohol alkane metabolic defenses enzyme activity assay after derivant process creeping bentgrass of the present invention:
2 of 250 μm of ol/L, 3-BD, water (contrast) process creeping bentgrass after 7 days, inoculation Rhizoctonia solani Kuhn, inoculate first 1 day and within the 1st, 3,5,7,9 day, sample respectively after inoculation, respectively with reference to the method for Latunde-Dada, Lister, Knobloch, measure the typical reaction enzyme of induced resistance: the change that phenylalnine ammonialyase (PAL), enzyme, namely chalcone isomerase (CHI), 4-coumarate CoA-ligase (4CL) are active.Result shows, 2, the mixture of 3-butanediol stereoisomer effectively can improve phenylalnine ammonialyase (PAL), enzyme, namely chalcone isomerase (CHI), 4-coumarate CoA-ligase (4CL) activity, wherein, latter 1st day of inoculation, phenylalnine ammonialyase (PAL) activity reaches 39.25Umin
-1g
-1fW; Latter 3rd day of inoculation, enzyme, namely chalcone isomerase (CHI) activity reaches 357.33Uh
-1g
-1fW; After inoculation, the 7th day 4-coumarate CoA-ligase (4CL) activity reaches 44.04Umin
-1g
-1fW, as shown in figs 6-8.
Embodiment 7:
Inoculate Rhizoctonia solani Kuhn after derivant process creeping bentgrass of the present invention, phenylpropyl alcohol alkane metabolic defenses is correlated with secondary metabolites content analysis:
2 of 250 μm of ol/L, after 3-BD, water treatment creeping bentgrass 7 days, inoculation Rhizoctonia solani Kuhn, within 3rd, 5,7,9,11 day, sample respectively after inoculation, respectively with reference to the method for Bonilla, Wolfe, Liu Guoqiang, measure the disease-resistant relevant secondary metabolites of phenylpropyl alcohol alkane metabolic pathway: the change of total phenol, flavonoids, content of lignin.The mixture of 2,3-butanediol stereoisomer can significantly improve the total phenol of creeping bentgrass, flavones and content of lignin, and wherein, to connect after bacterium the 5th day, total phenol content reaches 1.087mg/ (gFW); Latter 9th day of inoculation, flavones content reaches 0.027mg/ (gFW) respectively; To connect after bacterium the 11st day, content of lignin reaches 0.599OD respectively
280g
-1fW, as shown in figs. 9-11.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (5)
1. to produce the application in disease resistance at induction turfgrass based on the inducer of ISR mechanism, it is characterized in that, described derivant is 2,3-butanediol stereoisomer mixture, structure as shown in the formula (I),
Formula (I).
2. application according to claim 1, is characterized in that, described turfgrass is creeping bentgrass, kentucky blue grass, English ryegrass or Festuca Arundinacea.
3. application according to claim 2, is characterized in that, the method that applies of described derivant applies method for adopting root, and induction turfgrass produces disease resistance to typical disease (brown spot and fusarium wilt).
4. application according to claim 3, is characterized in that, the preferred concentration of described derivant induction creeping bentgrass Resistance to Cercospora is 250 μm of ol/L; The concentration of described derivant induction kentucky blue grass Resistance to Cercospora is 550 μm of ol/L; The preferred concentration of described derivant induction English ryegrass Resistance to Cercospora is 450 μm of ol/L; The preferred concentration of described derivant induction Festuca Arundinacea Resistance to Cercospora is 250 μm of ol/L.
5. application according to claim 3, is characterized in that, the preferred concentration of the anti-fusarium wilt of described derivant induction creeping bentgrass is 250 μm of ol/L.
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| CN201410717486.0A Pending CN104686512A (en) | 2014-12-02 | 2014-12-02 | Application of ISR mechanism based disease-resistant inductive agent in preventing and treating turfgrass disease |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2002032227A1 (en) * | 2000-10-17 | 2002-04-25 | Victorian Chemicals International Pty Ltd | Herbicide composition |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2002032227A1 (en) * | 2000-10-17 | 2002-04-25 | Victorian Chemicals International Pty Ltd | Herbicide composition |
Non-Patent Citations (3)
| Title |
|---|
| A.M.CORTES-BARCO ET AL: "Induced systemic resistance against three foliar diseases of Agrostis stolonifera by (2R,3R)-butanediol or an isoparaffin mixture", 《ANNALS OF APPLIED BIOLOGY》 * |
| CHOONG-MIN RYU ET AL: "Bacterial Volatiles Induce Systemic Resistance in Arabidopsis", 《PLANT PHYSIOLOGY》 * |
| JONATHAN TUNG: "The effectiveness of induced plant disease resistance:genotypic variation and quantification by chlorophyll fluorescence", 《A THESIS PRESENTED TO THE UNIVERSITY OF GUELPH》 * |
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Application publication date: 20150610 |