CN104678004A - Quality control method for kudzuvine root and hawthorn lipid-lowering particles - Google Patents
Quality control method for kudzuvine root and hawthorn lipid-lowering particles Download PDFInfo
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Abstract
The invention relates to a quality control method for kudzuvine root and hawthorn lipid-lowering particles, belonging to the technical field of pharmaceutical quality control. Quality control of the kudzuvine root and hawthorn lipid-lowering particles is achieved through a simple puerarin detection method and a method for measuring the contents of hawthorn general flavone and stilbeneglycoside. By means of multi-index content measurement, the quality control method disclosed by the invention is capable of evaluating the quality of the kudzuvine root and hawthorn lipid-lowering particles more comprehensively for ensuring the product quality stability, and the effectiveness and the safety for clinical medication. The quality control method disclosed by the invention has the characteristics of being simple in detection means, accurate in detection result and more comprehensive in quality supervision, and can satisfy the requirements of quick test and traditional Chinese medicine quality control and improvement better.
Description
Technical field
The invention belongs to technical field of medicine quality control, be specifically related to a kind of method of quality control of Ge Shan blood-fat-lowering granule.
Background technology
Ge Shan blood-fat-lowering granule comes from old docter of TCM Mr. Gao Daofan clinical experience side for many years of Chaohu, Anhui Province, China city, being the tcm product that a kind of In Patients With Primary Hyperlipoidemia (the stagnant double kidney deficiency and liver card of phlegm stasis blocking) to causing because of some disease, aging and functional disturbance has clear and definite curative effect, is also a kind of tcm product of the important auxiliary value to cardiovascular and cerebrovascular diseases such as hypertension, coronary heart disease, headstrokes.
At present, in the quality standard of Ge Shan blood-fat-lowering granule particle, only have the TLC distinguish item of prepared fleece flower root, the root of kudzu vine and the HPLC assay item of Puerarin, and the preparation of puerarin content working sample is comparatively complicated.This is difficult to embody this prescription compatibility of medicines feature, each monitoring requirement of flavour of a drug effective component and the demand of quick test to a great extent.Especially for have in side the flavour of a drug of different lipopenicillinase mechanism or effective constituent supervision on, existing quality standard in supervision scope and measure of supervision etc. on more shown serious deficiency.
Summary of the invention
The object of the invention is to the weak point overcoming the supervision of existing quality standard, provide a kind of detection means simple, the method of quality control of testing result Ge Shan blood-fat-lowering granule accurately, reaches the quality control to Ge Shan blood-fat-lowering granule by the simple detection method to Puerarin and the content assaying method to hawthorn fruit total flavone, Stibene-glucoside.
The object of the invention is to be achieved through the following technical solutions.
A method of quality control for Ge Shan blood-fat-lowering granule, is characterized in that, comprises the following steps.
(1) mensuration of puerarin content.
Chromatographic column: diameter and length are 4.6mm × 250mm, the Diamonsil C18 of 5 μm; Mobile phase: methanol-water (25: 75), flow velocity: 1.0ml/min, column temperature: 30 DEG C, determined wavelength: 250nm, sample size: 20 μ l, sampling time: 30min.
The preparation of reference substance solution: precision takes Puerarin reference substance 10mg, puts in 25ml measuring bottle, adds methyl alcohol to scale, shakes up to obtain reference substance solution (Puerarin 400ug/ml).
The preparation of need testing solution: get Ge Shan blood-fat-lowering granule, porphyrize, precision takes 1g, adds water saturated normal butyl alcohol and ethanol (4: 1) mixed liquor 50ml, add hot reflux 30 minutes, filter, the above-mentioned mixed solution 20ml of the dregs of a decoction washs, and washing lotion and filtrate merge, put evaporate to dryness in water-bath, residue mobile phase gradation is dissolved, and adds mobile phase and is settled to 100ml, as need testing solution.
The selection of determined wavelength: precision takes Puerarin reference substance 10mg, adds methyl alcohol preparation Puerarin 400ug/ml.In the interscan of 200 ~ 400nm scope, determine the maximum absorption wavelength λ of Puerarin
maxfor 250nm.
The investigation of linear relationship: accurate absorption Puerarin reference substance solution 0.25ml, 0.5ml, 0.75ml, 1ml, 1.25ml, 1.5ml, 2.0ml respectively, add methyl alcohol to 10ml, sample introduction 20 μ l, record chromatogram, with Puerarin concentration (C), linear regression is done to peak area (A), obtain equation C=6.75 × 10
-9a-0.2136, R=0.9997.
The investigation of precision: the Puerarin contrast solution of 80 μ g/ml under line taking sexual intercourse item, continuous sample introduction 6 pin, every pin 20 μ l, reads peak area, and calculating RSD is 0.64%.
Reproducible investigation: get test sample solution, continuous sample introduction 6 pin, every pin 20 μ l, reads peak area, and calculate content, RSD is 1.07%.
The investigation of stability: get lot number 20120319 need testing solution, respectively at sample introduction 20ul when 0h, 2h, 4h, 8h, 16h, 24h, read peak area, obtaining RSD is 1.61%.
The investigation of the recovery: sample thief 1.5g prepares sample solution 150ml, draw 5ml, 10ml, 15ml, 20ml, 25ml, 30ml respectively, add corresponding amount Puerarin reference substance respectively, add mobile phase and be settled to 50ml, by method operation under assay item, recording Puerarin average recovery rate is 99.94, RSD=2.37%.
Assay: (20 μ l, under above-mentioned condition, injection liquid chromatography, measures for accurate absorption Puerarin reference substance (Puerarin 40 μ g/ml) and need testing solution.
The mensuration of Ge Shan blood-fat-lowering granule Puerarin limit: prepare 10 batch sample test liquids, above method measures Ge Shan blood-fat-lowering granule puerarin content, and obtaining average content is 20.54mg/g, determines that puerarin content limit is 20.54mg/g × 70%=14.38mg/g.
(2) mensuration of general flavone.
The preparation of reference substance solution: get rutin standard items 5.12mg and put 10ml volumetric flask, add 50% methanol constant volume, make the reference substance solution that concentration is 0.512mg/ml.
The preparation of need testing solution: get Ge Shan blood-fat-lowering granule appropriate, porphyrize, precision takes 1g, adds 50% methanol eddy and extracts 1.5 hours.The methanol constant volume of cold rear use 50%, to 50ml, is filtered, and collects subsequent filtrate and makes need testing solution.
The selection of determined wavelength: get the control substance of Rutin that concentration is 0.512mg/ml, the interscan of 400 ~ 600nm scope, determines that flavones maximum absorption wavelength λ max is 510nm.
The foundation of typical curve: accurately draw rutin titer 0 that concentration is 0.512mg/ml, 1.0,2.0,3.0,4.0,5.0,6.0ml, be placed in 25ml volumetric flask, accurately add 5%NaNO
2solution 1ml, shakes up, and places 5min, more accurately adds 10%Al (NO
3)
3solution 1ml, places 5min, then adds 4ml NaOH, with 50% methanol dilution to scale, after reacting 10min, make blank reference with the solution not adding rutin titer, survey its absorbance respectively in 510nm place in 60 DEG C of water-baths.Calculate regression equation A=0.1217C-0.0151, coefficient R
2=0.9994.
Color stability is tested: get rutin standard solution (71.68 μ g/ml) and need testing solution 0.02g/ml, measure its absorbance, totally 6 times every 10min, and analyze and measure general flavone content, RSD is all lower than 3%.
Precision Experiment: under line taking sexual intercourse item, concentration is the contrast solution of 71.68ug/ml, as stated above, METHOD FOR CONTINUOUS DETERMINATION 6 times, record chromatogram, reads peak area, and calculating RSD is 0.64%.
The recovery is tested: sample thief (lot number 20120319) 3g prepares sample solution and is about 150ml, draw 5ml, 10ml, 15ml, 20ml, 25ml, 30ml respectively, add corresponding amount control substance of Rutin respectively, add 50% methanol dilution and be settled to 50ml, by method operation under assay item, recording rutin average recovery rate is 99.89%, RSD=1.21%.
The mensuration of general flavone content limitation: by above-mentioned test sample and control substance of Rutin compound method test preparation solution, measure 10 batches of Ge Shan blood-fat-lowering granule general flavone contents, obtaining general flavone average content is 73.73mg/g, determines that general flavone content limit is 73.73mg/g × 70%=51.61mg/g.
(3) mensuration of Stibene-glucoside content.
Chromatographic column: diameter and length are 4.6mm × 250mm, the Diamonsil C18 of 5 μm; Mobile phase: acetonitrile-water (22:78), flow velocity: 1ml/min, column temperature: 30 DEG C, determined wavelength: 320nm, sample size: 20 μ l, sampling time: 30min.
The preparation precision of reference substance solution takes 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-Glucose glycosides reference substance 10mg, puts in 25ml measuring bottle, adds methyl alcohol to scale, shakes up to obtain reference substance (every 1ml is containing Stibene-glucoside 400ug).
The preparation of need testing solution: the sample got respectively under each batch of content uniformity is appropriate, and porphyrize, precision takes 1g, adds 50ml 50% methyl alcohol ultrasonic dissolution, gets subsequent filtrate 25ml and adds 50% methanol constant volume to 50ml, as need testing solution.
The determination of determined wavelength: get Stibene-glucoside reference substance solution (every 1ml is containing 400ug).With 50% methyl alcohol for blank correction, in the interscan of 200 ~ 400nm scope, determine that the maximum absorption wavelength λ max of Stibene-glucoside is 320nm.
The investigation of linear relationship: accurate absorption Stibene-glucoside reference substance solution 0.25ml, 0.5ml, 1ml, 1.5ml, 2ml, 2.5ml, 3.0ml respectively, be placed in 10ml measuring bottle, add 50% methyl alcohol to scale, shake up, sample introduction 20 μ l, read peak area, obtain equation C=3 × 10-8A-0.0075, R=0.9998.
The investigation of precision: under line taking sexual intercourse item, concentration is 60ug/ml contrast solution, by upper method, continuous sample introduction 6 pin, every pin 20ul, reads peak area, and calculating RSD is 0.91%.
Reproducible investigation: get test sample solution, continuous sample introduction 6 pin, every pin 20ul, read peak area, obtaining RSD is 1.44%.
The investigation of stability: get test liquid, respectively at sample introduction 20ul when 0h, 2h, 4h, 8h, 16h, 24h, read peak area, calculating RSD is 2.6%.
The investigation of the recovery: sample thief 1.5g prepares sample 150ml, draw 5ml, 10ml, 15ml, 20ml, 25ml, 30ml respectively, add the Stibene-glucoside reference substance of corresponding amount respectively, add mobile phase and be settled to 50ml, recording Stibene-glucoside average recovery rate is 99.94, RSD=2.37%.
Assay: accurate absorption Stibene-glucoside reference substance and need testing solution 20 μ l, injection liquid chromatography, measures, the results are shown in Figure 2.
The investigation of Ge Shan blood-fat-lowering granule Stibene-glucoside limit: prepare 10 batch sample test liquids, it is 17.928mg/g that above method mensuration Ge Shan blood-fat-lowering granule Stibene-glucoside obtains average content, determines that Stibene-glucoside content limit is 17.928mg/g × 70%=12.55mg/g.
The invention has the beneficial effects as follows: the present invention by multi objective assay, can evaluate the quality of Ge Shan blood-fat-lowering granule more all sidedly, ensure the stability of product quality and the validity of clinical application and security.The present invention has that detection means is simple, testing result accurately, quality supervision more comprehensively feature, more can be applicable to the requirement that quick test and traditional Chinese medicine quality control to improve.
Accompanying drawing explanation
Fig. 1: Puerarin reference substance HPLC image detect result in embodiment.
Fig. 2: Stibene-glucoside reference substance HPLC image detect result in embodiment.
Fig. 3: embodiment Ge Shan blood-fat-lowering granule (lot number: 20120319) study on the stability result.
Result is investigated in Fig. 4: Ge Shan blood-fat-lowering granule HPLC finger-print optimization.
Fig. 5: Ge Shan blood-fat-lowering granule HPLC finger-print optimization is investigated Puerarin reference substance and is investigated result.
Fig. 6: Ge Shan blood-fat-lowering granule HPLC finger-print optimization is investigated Stibene-glucoside reference substance and is investigated result.
Specific embodiment
Elaborate technical scheme of the present invention below by embodiment and accompanying drawing thereof, the embodiment exemplified and accompanying drawing do not limit the present invention.
1. the content assaying method of Puerarin is:
1.1 samples, instrument and reagent.
Ge Shan blood-fat-lowering granule is (by green because of the preparation of medicine company Ge Shan blood-fat-lowering granule technique; Lot number 20120319,20120325,20120401,20120407,20120411,20120413,20120417,20120420,20120424,20120430), Puerarin reference substance (Nat'l Pharmaceutical & Biological Products Control Institute).
Waters1525 type high performance liquid chromatograph, Waters2489 type detecting device, SHIMADZU AUW120D 100,000/balance, XMTD-4000 type constant water bath box (Yongming, Beijing Medical Instruments company limited); TU-1810 ultraviolet-visible pectrophotometer.
Chromatogram methyl alcohol (Tianjin Heng Xing chemical reagent Manufacturing Co., Ltd, lot number: 20130901); Water is distilled water; Normal butyl alcohol (Tianjin Fu Yu Fine Chemical Co., Ltd, lot number: 100623); Ethanol (the same normal butyl alcohol of producer, lot number: 20121006).
1.2 methods and result.
1.2.1 the selection of determined wavelength.
Precision takes Puerarin reference substance 10mg, puts in 25ml measuring bottle, adds methyl alcohol to scale, shakes up to obtain reference substance (Puerarin 400ug/ml).Take methyl alcohol as blank correction, in the interscan of 200 ~ 400nm scope, determine the maximum absorption wavelength λ of Puerarin
maxfor 250nm.
1.2.2 the determination of chromatographic condition.
Chromatographic column: diameter and length are 4.6mm × 250mm, the Diamonsil C18 of 5 μm; Mobile phase: methanol-water (25:75), flow velocity: 1.0ml/min, column temperature: 30 DEG C, determined wavelength: 250nm, sample size: 20 μ l, sampling time: 30min.
1.2.3 the preparation of reference substance solution.
Precision takes Puerarin reference substance 10mg, puts in 25ml measuring bottle, adds methyl alcohol to scale, shakes up to obtain reference substance (Puerarin 400ug/ml).
1.2.4 the preparation of need testing solution.
Get this product under each batch of content uniformity respectively, porphyrize, precision takes 1g, adds water saturated normal butyl alcohol and ethanol (4:1) mixed solution 50ml, add hot reflux 30 minutes, filter, the above-mentioned mixed solution 20ml of the dregs of a decoction washs, and washing lotion and filtrate merge, put evaporate to dryness in water-bath, residue mobile phase gradation is dissolved, and adds mobile phase and is settled to 100ml, as need testing solution.
1.2.5 the investigation of linear relationship.
Accurate absorption Puerarin reference substance solution 0.25ml, 0.5ml, 0.75ml, 1ml, 1.25ml, 1.5ml, 2.0ml respectively, put in 10ml measuring bottle, add methyl alcohol to scale, shake up, sample introduction 20 μ l, record chromatogram, reads peak area.
With Puerarin concentration (C), linear regression is done to peak area (A), obtain equation C=6.75 × 10
-9a-0.2136, R=0.9997.From linear equation, Puerarin in 10 μ g/ml ~ 160 μ g/ml concentration ranges, in good linear relation.
1.2.6 the investigation of precision.
Under line taking sexual intercourse item, concentration is the contrast solution of 80ug/ml, and as stated above, continuous sample introduction 6 pin, every pin 20ul, reads peak area, and calculating RSD is 0.64%, and known this method precision is good.
1.2.7 reproducible investigation.
Get lot number 20120319 test sample solution, continuous sample introduction 6 pin, every pin 20ul, measure, record chromatogram, reads peak area, and calculate content, RSD is 1.07%, and known this method reappearance is good.
1.2.8 the investigation of stability.
Get lot number 20120319 test sample solution, respectively at sample introduction 20ul when 0h, 2h, 4h, 8h, 16h, 24h, measure, record chromatogram, reads peak area, and calculate content, RSD is 1.61%, and known this method has good stability.
1.2.9 the investigation of the recovery.
Sample thief (lot number 20120319) 1.5g prepares sample 150ml, draws 5ml, 10ml, 15ml, 20ml, 25ml, 30ml respectively, adds the Puerarin reference substance of corresponding amount respectively; Add mobile phase and be settled to 50ml, by method operation under assay item, recording Puerarin average recovery rate is 99.94, RSD=2.37%, and known this method recovery is better.
1.2.10 the mensuration of Ge Shan blood-fat-lowering granule Puerarin limit.
Prepare each batch sample test liquid by above-mentioned test sample compound method, above method measures 10 batches of Ge Shan blood-fat-lowering granule puerarin contents.Obtaining average content is 20.54mg/g, determines that puerarin content limit is 20.54mg/g × 70%=14.38mg/g, in table 1.
The measurement result of table 1 different batches Ge Shan blood-fat-lowering granule puerarin content.
2. Determination Method of Flavone Content is:
2.1 samples, instrument and reagent.
Ge Shan blood-fat-lowering granule (sample is with puerarin content assay method sample); Control substance of Rutin (Nat'l Pharmaceutical & Biological Products Control Institute).
TU-1810 ultraviolet-visible pectrophotometer, SHIMADZU AUW120D 100,000/balance, XMTD-4000 type constant water bath box (Yongming, Beijing Medical Instruments company limited).
(water is distilled water to methyl alcohol for Tianjin Heng Xing chemical reagent Manufacturing Co., Ltd, lot number: 20121101); 5%NaNO
2solution; 1N NaOH solution; 10%AL (NO3) 3 solution.
2.2 methods and result.
2.2.1 the preparation of sample.
Get each batch of this product respectively appropriate, porphyrize, gets 1g, accurately weighed, adds 50% methanol eddy and extracts 1.5 hours.Let cool, by 50% methanol constant volume to 50ml, filter, collect subsequent filtrate and make test solution.
2.2.2 the preparation of reference substance.
Get rutin standard items 5.12mg and put 10ml volumetric flask, add 50% methanol constant volume, make the standard solution that concentration is 0.512mg/ml.
2.2.3 the selection of determined wavelength.
Get the rutin standard items working fluid that concentration is 0.512mg/ml, the interscan of 400 ~ 600nm scope, determine that flavones maximum absorption wavelength λ max is 510nm.
2.2.4 the foundation of typical curve.
Accurate absorption concentration be 0.512mg/ml rutin titer 0,1.0,2.0,3.0,4.0,5.0,6.0ml, be placed in 25ml volumetric flask, accurately add 5%NaNO
2solution 1ml, shakes up, and places 5min, more accurately adds 10%AL (NO
3)
3solution 1ml, places 5min, then adds 4ml 1N NaOH, with 50% methanol dilution to scale, after reacting 10min, make blank reference with the solution not adding rutin titer, survey its absorbance respectively in 510nm place in 60 DEG C of water-baths.Drawing standard curve, calculates regression equation A=0.1217C-0.0151, coefficient R
2=0.9994.From linear equation, rutin in 20.48 μ g/ml ~ 122.88 μ g/ml concentration ranges, in good linear relation.
2.2.5 color stability experiment.
Get rutin standard solution and need testing solution, under standard curve determination condition, rutin is at 71.68 μ g/ml, measures its absorbance every 10min, totally 6 times, analyze and measure general flavone content, RSD, all lower than 3%, shows rutin titer and need testing solution 1h color stability.
2.2.6 Precision Experiment.
Under line taking sexual intercourse item, concentration is the contrast solution of 71.68ug/ml, and as stated above, METHOD FOR CONTINUOUS DETERMINATION 6 times, record chromatogram, reads peak area, and calculating RSD is 0.64%, and known this method precision is good.
2.2.7 recovery experiment.
Sample thief (lot number 20120319) 3g prepares sample solution and is about 150ml, draw 5ml, 10ml, 15ml, 20ml, 25ml, 30ml respectively, add corresponding amount control substance of Rutin respectively, add 50% methanol dilution and be settled to 50ml, by method operation under assay item, recording rutin average recovery rate is 99.89%, RSD=1.21%, and known this method recovery is better.
2.2.8 the mensuration of general flavone content limitation.
By above-mentioned test sample and control substance of Rutin compound method test preparation solution, above method measures 10 batches of Ge Shan blood-fat-lowering granule general flavone contents.Obtaining average content is 73.73mg/g, determines that general flavone content limit is 73.73mg/g × 70%=51.61mg/g, in table 2.
The measurement result of table 2 different batches sample general flavone.
3. the content assaying method of Stibene-glucoside is:
3.1 sample, instrument and reagent.
Ge Shan blood-fat-lowering granule (sample is with puerarin content assay method sample), 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-Glucose glycosides reference substance (Nat'l Pharmaceutical & Biological Products Control Institute).
Waters1525 type high performance liquid chromatograph, Waters2489 type detecting device, SHIMADZU AUW120D 100,000/balance, XMTD-4000 type constant water bath box (Yongming, Beijing Medical Instruments company limited); TU-1810 ultraviolet-visible pectrophotometer.
Chromatogram acetonitrile (Fu Yu Fine Chemical Co., Ltd of Jinshi City, lot number: 121022); Water is distilled water; Chromatogram methyl alcohol (Tianjin Heng Xing chemical reagent Manufacturing Co., Ltd, lot number: 20130901).
3.2 methods and result.
3.2.1 the selection of determined wavelength.
Precision takes 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-Glucose glycosides reference substance 10mg, puts in 25ml measuring bottle, adds methyl alcohol to scale, shakes up to obtain reference substance (every 1ml is containing Stibene-glucoside 400ug).Take methyl alcohol as blank correction, in the interscan of 200 ~ 400nm scope, determine that the maximum absorption wavelength λ max of Stibene-glucoside is 320nm.
3.2.2 the determination of chromatographic condition.
Chromatographic column: diameter and length are 4.6mm × 250mm, the Diamonsil C18 of 5 μm; Mobile phase: acetonitrile-water (22:78), flow velocity: 1ml/min, column temperature: 30 DEG C, determined wavelength: 320nm, sample size: 20 μ l, sampling time: 30min.
3.2.3 the preparation of reference substance solution.
Precision takes 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-Glucose glycosides reference substance 10mg, puts in 25ml measuring bottle, adds methyl alcohol to scale, shakes up to obtain reference substance (every 1ml is containing Stibene-glucoside 400ug).
3.2.4 the preparation of need testing solution.
The sample got respectively under each batch of content uniformity is appropriate, and porphyrize, precision takes 1g, adds 50ml 50% methyl alcohol ultrasonic dissolution, gets subsequent filtrate 25ml and adds 50% methanol constant volume to 50ml, as need testing solution.
3.2.5 linear relationship is investigated.
Accurate absorption Stibene-glucoside reference substance solution 0.25ml, 0.5ml, 1ml, 1.5ml, 2ml, 2.5ml, 3.0ml respectively, be placed in 10ml measuring bottle, add 50% methyl alcohol to scale, shake up, sample introduction 20 μ l, record chromatogram, reads peak area.
With Stibene-glucoside concentration (C), linear regression is done to peak area (A), obtain equation C=3 × 10-8A-0.0075, R=0.9998.From linear equation, Stibene-glucoside in 10 μ g/ml ~ 120 μ g/ml concentration ranges, in good linear relation.
3.2.6 the investigation of precision.
Under line taking sexual intercourse item, concentration is the contrast solution of 60ug/ml, and as stated above, continuous sample introduction 6 pin, every pin 20ul, record chromatogram, reads peak area, and calculating RSD is 0.91%, and known this method precision is good.
3.2.7 reproducible investigation.
Get lot number 20120319 test sample solution, continuous sample introduction 6 pin, every pin 20ul, measure peak area, obtaining RSD is 1.44%, and known this method reappearance is good.
3.2.8 the investigation of stability.
Get lot number 20120319 test sample solution, respectively at sample introduction 20ul when 0h, 2h, 4h, 8h, 16h, 24h, measure, read peak area, calculating RSD is 2.6%, and known this method has good stability.
3.2.9 the investigation of the recovery.
Sample thief (lot number 20120319) 1.5g prepares sample 150ml, draw 5ml, 10ml, 15ml, 20ml, 25ml, 30ml respectively, add the Stibene-glucoside reference substance of corresponding amount respectively, add mobile phase and be settled to 50ml, by method operation under assay item, recording Stibene-glucoside average recovery rate is 99.94, RSD=2.37%, and known this method recovery is better.
3.2.10 the investigation of Ge Shan blood-fat-lowering granule Stibene-glucoside content limitation.
Prepare need testing solution by test sample preparation method under Stibene-glucoside content assaying method, measure the content of 10 batches of Ge Shan blood-fat-lowering granule Stibene-glucosides with said method.Obtaining average content is 17.928mg/g, determines that Stibene-glucoside content limit is 17.928mg/g × 70%=12.55mg/g.In table 3.
The measurement result of table 3 different batches sample Stibene-glucoside.
Claims (4)
1. a method of quality control for Ge Shan blood-fat-lowering granule, is characterized in that: by what realize the mensuration of preparation effective constituent Puerarin, general flavone, Stibene-glucoside content.
2. the method for quality control of a kind of Ge Shan blood-fat-lowering granule according to claim 1, is characterized in that: the content assaying method of described Puerarin is take Puerarin as the HPLC determination method of standard items:
(1) preparation method of reference substance is: precision takes Puerarin reference substance 1mg, puts in 25ml measuring bottle, adds methyl alcohol to scale, shakes up to obtain reference substance solution Puerarin 40ug/ml;
(2) preparation method of test sample is: get Ge Shan blood-fat-lowering granule, porphyrize, precision takes 1g, add water saturated normal butyl alcohol and alcohol mixeding liquid 50ml, normal butyl alcohol: ethanol=4: 1, adds hot reflux 30 minutes, filters, the above-mentioned mixed solution 20ml of the dregs of a decoction washs, washing lotion and filtrate merge, and put evaporate to dryness in water-bath, and residue mobile phase gradation is dissolved, add mobile phase and be settled to 100ml, as need testing solution;
(3) chromatographic condition is: chromatographic column: diameter and length are 4.6mm × 250mm, the Diamonsil C18 of 5 μm; Mobile phase: methanol-water, methyl alcohol: water=5: 75, flow velocity: 1.0ml/min, column temperature: 30 DEG C, determined wavelength: 250nm, sample size: 20 μ l, sampling time: 30min;
(4) puerarin content defining method is: measure 10 batches of Ge Shan blood-fat-lowering granule puerarin contents, obtain Puerarin average content × 70%.
3. the method for quality control of a kind of Ge Shan blood-fat-lowering granule according to claim 1, is characterized in that: the content assaying method of described general flavone is take rutin as the ultraviolet spectrophotometry of reference substance:
(1) preparation method of reference substance solution is: get rutin standard items 5.12mg and put 10ml volumetric flask, add 50% methanol constant volume, make the reference substance solution that concentration is 0.512mg/ml;
(2) preparation method of need testing solution is: get Ge Shan blood-fat-lowering granule appropriate, porphyrize, precision takes 1g, adds 50% methanol eddy and extracts 1.5 hours, and cold rear use 50% methanol constant volume, to 50ml, is filtered, and collects subsequent filtrate and makes need testing solution;
(3) detection method of content is: ultraviolet-visible spectrophotometry is in rutin maximum absorption wavelength λ
maxgeneral flavone content is detected at=510nm place;
(4) general flavone content defining method is: measure 10 batches of Ge Shan blood-fat-lowering granule general flavone contents, obtain general flavone average content × 70%.
4. the method for quality control of a kind of Ge Shan blood-fat-lowering granule according to claim 1, is characterized in that: the content assaying method of described Stibene-glucoside is take Stibene-glucoside as the HPLC determination method of standard items:
(1) preparation of reference substance solution: precision takes 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-Glucose glycosides reference substance 10mg, puts in 25ml measuring bottle, adds methyl alcohol to scale, shake up to obtain reference substance, every 1ml is containing Stibene-glucoside 400ug;
(2) preparation of need testing solution: get Ge Shan blood-fat-lowering granule appropriate, porphyrize, precision takes 1g, adds 50ml 50% methyl alcohol ultrasonic dissolution, gets subsequent filtrate 25ml and adds 50% methanol constant volume to 50ml, as need testing solution;
(3) chromatographic column: diameter and length are 4.6mm × 250mm, the Diamonsil C18 of 5 μm; Mobile phase: acetonitrile-water, acetonitrile: water=22: 78, flow velocity: 1ml/min, column temperature: 30 DEG C, determined wavelength: 320nm, sample size: 20 μ l, sampling time: 30min;
(4) Stibene-glucoside content defining method is: measure 10 batches of Ge Shan blood-fat-lowering granule Stibene-glucoside content, obtain Stibene-glucoside average content × 70%.
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| CN201410365250.5A Active CN104678004B (en) | 2014-07-29 | 2014-07-29 | A kind of method of quality control of Ge Shan blood-fat-lowering granule |
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| CN114720600A (en) * | 2022-04-07 | 2022-07-08 | 广东安诺药业股份有限公司 | Method for detecting stilbene glucoside component in polygonum multiflorum prepared by bone-protecting capsule |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106769964A (en) * | 2016-12-09 | 2017-05-31 | 中国科学院新疆理化技术研究所 | The method of quality control of Herba Achilleae extract |
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| CN107349213A (en) * | 2017-07-10 | 2017-11-17 | 苏州神瑞生物科技有限公司 | Applied based on the synthesising preparation of Stibene-glucoside in treatment nerve degenerative diseases medicine is prepared |
| CN111213882A (en) * | 2020-03-11 | 2020-06-02 | 北京康立生医药技术开发有限公司 | Plant mixed extraction process and application thereof |
| CN114720600A (en) * | 2022-04-07 | 2022-07-08 | 广东安诺药业股份有限公司 | Method for detecting stilbene glucoside component in polygonum multiflorum prepared by bone-protecting capsule |
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| CN104678004B (en) | 2016-09-07 |
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