CN104666278B - A kind of preparation and application with light-operated release function magnetic target medicine carrier - Google Patents
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Abstract
The present invention relates to a kind of preparation and application with light-operated release function magnetic target medicine carrier, and in particular to the preparation and application of the temperature sensitive chorion structure carrier with light-operated release function based on ferroso-ferric oxide, belongs to diagnosis and treatment agent field；The present invention is by magnetic iron oxide Surface coating silica, utilize temperature sensing material N N-isopropylacrylamides (PNIPAM) and N vinyl pyrrolidones (NVP) crosslinking coated silica, corrosion is carried out to silica by NaOH again and forms chorion structure carrier, the carrier phase-change temperature is about 41 DEG C, for loading anticancer drugs, doxorubicin；The application is that carrier has good magnetic targeted function, in near-infrared laser (NIR, the nm of wavelength 808) the lower generation photo-thermal effect of irradiation, induce temperature sensitive carrier to produce phase transformation at about 41 DEG C and discharge medicine, carrier prepared by the present invention is by magnetic resonance imaging, the functions such as photoacoustic imaging are integrated in one, and good application effect is shown in cell experiment, are a kind of good pharmaceutical carriers.
Description
Technical field
The present invention relates to a kind of preparation and application with light-operated release function magnetic target medicine carrier, and in particular to four
The preparation and application of the temperature sensitive chorion structure carrier with light-operated release function based on Fe 3 O, belong to diagnosis and treatment agent neck
Domain.
Background technology
Tumour is to endanger the big disease of human life and health, due to its complexity, existing operation, the methods of chemotherapy
It is difficult to reach good therapeutic effect, it is urgent problem to be solved to seek treatment means efficient, less toxic, that targeting is controllable.
Magnetic nano particle with its unique magnetic responsiveness, biocompatibility is high the features such as be widely used in biological medicine neck
Domain.Magnetic targeting drug delivery system can be such that medicine is effectively enriched with tumor locus, and the general toxicity of medicine is greatly lowered.Magnetic simultaneously
Property nanometer has good superparamagnetism, and it can be used for the diagnosis of disease as magnetic resonance developer.Research is found recently, magnetic
Property nanoparticle has good effect as new light heat sensitizer.Because near infrared light is non-toxic, institute good to penetration into tissue
Had broad application prospects using near infrared light as preferable excitation source in photothermal conversion.Light heat sensitizer can will be specific
Luminous energy changes into heat energy, existing studies have shown that Fe3O4Nanoparticle as novel thermosensitive agent, have significant effect (referring to：
Chen H, Burnett J, Zhang F, et al. Highly crystallized iron oxide
nanoparticles as effective and biodegradable mediators for photothermal
cancer therapy[J]. Journal of Materials Chemistry B, 2013, 7: 757-765.； Chu
M, Shao Y, Peng J. Near-infrared laser light mediated cancer therapy by
photothermal effect of Fe3O4 magnetic nanoparticles[J]. Biomaterials, 2013,
34: 4078-4088.)。
NIPA（PNIPAM）It is a kind of temperature sensitive type polymeric material, in lowest critical solution temperature
LCST makes the controlled release that reversible swelling-retraction volume changes thus can realize medicine up and down.Human body temperature is generally tieed up
Hold at 37 DEG C, and the LCST of simple NIPA is about 32 DEG C, therefore induce the LCST liters of Thermo-sensitive carrier
It is high significant.PNIPAM is modified by NVP (NVP), its LCST is increased to 41 DEG C of left sides
It is right.By Fe3O4Nanoparticle is combined with thermo-sensitive gel, and carrier further is prepared into chorion structure, loads anticancer drugs, doxorubicin,
The useful load of effective increase medicine.Prepared carrier is under near infrared light, Fe3O4Luminous energy is changed into heat by nanoparticle
Can, when temperature is more than LCST, thermo-sensitive gel drastically shrinkage, reach the effect for discharging medicine.
The content of the invention
The purpose of the present invention is to be that providing one kind has the temperature sensitive chorion carrier of light-operated release function, i.e., with light-operated release
The preparation method of function magnetic target medicine carrier.
Another object of the present invention is to be to provide a kind of application of above-mentioned carrier.
Application of the present invention is that the carrier is used to load anticancer drugs, doxorubicin.
Further, described application is that the carrier has magnetic, under additional magnetic fields, has targeting.
Further, described application is that the carrier can turn near infrared light under the irradiation of the nm laser of near infrared light 808
Heat energy is melted into, temperature rises to about 41 DEG C and causes temperature sensing material phase transformation, promotes insoluble drug release, and produce killing to tumour cell and make
With.
The present invention solves the concrete scheme that above-mentioned technical problem is taken：
A kind of preparation method with light-operated release function magnetic target medicine carrier, is followed the steps below：
（1）Fe3O4 Prepare：
FeCl3·6H2O is soluble in water, adds ethanol, n-hexane, oleic acid, stirs 30 min, add NaOH,
4 h are stirred after closed at 70 DEG C, separatory funnel separates solution, and upper organic layer deionized water rinsing is more
It is secondary, at 80 DEG C overnight, with evaporation of hexane；Obtained product is dispersed in oleic acid and 1- octadecylene mixed solutions, N2Degassing 30
Min, in logical N2In the case of, it is heated to 320 DEG C of min of stirring reaction 30；Put to room temperature, ethanol-n-hexane alternating centrifugal punching
Wash repeatedly, obtained solid distributed and saved is in n-hexane.
（2）Fe3O4@SiO2It is prepared by nanoparticle：
Fe is prepared by reverse micelle of microemulsion3O4@SiO2Nanoparticle, Igepal CO-520 are dispersed in hexamethylene, stirring 30
~ 40 min, step（1）Obtained Fe3O4Cyclohexane solution is dissolved in, is added in above-mentioned solution and is stirred continuously 3 h, adds ammonia
Water, 5 ~ 10 min are stirred, add tetraethyl orthosilicate（TEOS）；Alcohol-water alternately rinses twice, preserves in ethanol.
Wherein, the volume ratio of Igepal CO-520 and hexamethylene is 1：21；
Fe3O4The concentration of cyclohexane solution is 1 mg/mL；
Fe3O4The volume of cyclohexane solution, ammoniacal liquor and TEOS is 8 ~ 12 mL：325 µL：150~500 µL；Preferably 10
mL：325 µL：150~500 µL；Every 16 h of wherein TEOS add 75 μ L.
TEOS amounts are different, prepared Fe3O4@SiO2The SiO of nanoparticle2Thickness degree is different, prepared by the present invention
Fe3O4@SiO2The SiO of nanoparticle2Thickness degree is 12 ~ 25 nm.
（3）Build Fe3O4@SiO2Three layers of temperature sensitive carriers of-p (NIPAM-co-NVP)：
MPS is added to step（2）Obtained Fe3O4 @SiO2After the h of ethanol solution stirring reaction 4, alcohol flushing 2 ~ 3
Time, preserve in ethanol；NIPA (PNIPAM) and NVP (NVP) are soluble in water, add
Enter the Fe of above-mentioned MPS modifications3O4@SiO2Solution, the min of vacuum outgas 10 ~ 20, add N, N, N, N- tetramethylethylenediamines
(TMEDA), ammonium persulfate solution, the min of nitrogen 15 ~ 20 is led to, 70 °C of 4 h of waters bath with thermostatic control reaction after sealing；By product magnetic
Separation, deionized water rinsing 2 ~ 3 times, is stored in water, obtains Fe3O4@SiO2- p (NIPAM-co-NVP) solution.
Wherein, Fe3O4 @SiO2Ethanol solution concentration is 1 mg/mL；
MPS and Fe3O4 @SiO2Volumes of aqueous ethanol ratio is 1: 100；
The ratio of PNIPAM, NVP and water is 1g：0.5 mL：125 mL；
TMEDA, ammonium persulfate solution volume ratio are 1：10, wherein ammonium persulfate solution mass volume ratio is 10%, persulfuric acid
Ammonium salt solution ratio of the total volume is 1% ~ 3%, and optimal is 2%.
（4）Build Fe3O4- p (NIPAM-co-NVP) chorion particle：
Completed by silicon corrosion process：NaOH is added to Fe3O4@SiO2In-p (NIPAM-co-NVP) solution, at room temperature
6 ~ 8 h are stirred, centrifugation, are washed 2 ~ 3 times, described NaOH concentration is 0.8-1.5 M, and optimal concentration is 1 M, NaOH and Fe3O4@
SiO2The volume ratio of-p (NIPAM-co-NVP) solution is 1: 4.
The application of carrier of the present invention, concrete application step are as follows：
Fe3O4- p (NIPAM-co-NVP) (1 mg/mL) the chorion particle aqueous solution and the adriamycin aqueous solution (1 mg/mL)
24 h of mixing concussion；The carrier is 1 with adriamycin aqueous solution volume ratio：4.
Novel carriers prepared by the present invention have following three points technological value：
（1）The temperature sensitive carrier of ferroso-ferric oxide chorion prepared by the present invention can be as a kind of good pharmaceutical carrier, toxicity
It is low, the advantages that good biocompatibility.
（2）The temperature sensitive carrier of ferroso-ferric oxide chorion prepared by the present invention has magnetic, under additional magnetic fields, can assemble
In privileged site.
（3）The temperature sensitive carrier of ferroso-ferric oxide chorion prepared by the present invention has light-operated release function, ferroso-ferric oxide particle
Near infrared light can be absorbed and change into heat energy, cause temperature sensitive carrier phase-change to shrink, have the function that rapid delivery of pharmaceuticals.
Brief description of the drawings
The temperature sensitive chorion carrier transmission electron microscope pictures (TEM) of Fig. 1；In figure, a) Fe3O4 Nanoparticle；b) Fe3O4@SiO2 Grain
Son；c) Fe3O4@SiO2- p (NIPAM-co-NVP) three layers of particles；d) Fe3O4- p (NIPAM-co-NVP) chorion particle.
The temperature sensitive chorion carrier of Fig. 2 various concentrations temperature variation when 808 nm laser irradiate；Concentration is 200,100,
50,20,10,5 μ g/mL, abscissa are the min of irradiation time 1,2,3,4,5,6,7,8, and ordinate is temperature.
Release figure of Fig. 3 near infrared lights induced drugs in different pH value PBS solutions.
Fig. 4 carriers are under externally-applied magnetic field, magnetic target medicine therapeutic effect figure；1,2,3 be respectively apart from magnet in figure
Diverse location, it can be seen that nearer apart from magnet, cell mortality is higher.
The light-operated insoluble drug releases of Fig. 5 are to cytotoxicity design sketch；In figure a) carrier after NIR pre-irradiations to cell
Toxicity；B) adriamycin, pharmaceutical carrier and pharmaceutical carrier add after laser pre-irradiation to the toxicity of cell；Fe3O4- in figure
PNIPAM is Fe3O4-p (NIPAM-co-NVP) abbreviation.
Embodiment
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This.
The Fe of embodiment 13O4 Prepare
0.54 g FeCl3·6H2O （2 mmol）It is dissolved in 6 mL water, 8 mL ethanol of addition, 14 mL n-hexanes,
1.9 mL oleic acid, 30 min are stirred, add 0.24 g NaOH, stir 4 h, separatory funnel separation after closed at 70 DEG C
Solution, upper organic layer deionized water rinsing is multiple, at 80 DEG C overnight, with evaporation of hexane.Obtained product is dispersed in
In 0.32 mL oleic acid and 12.5 mL 1- octadecylene mixed solutions, N2Deaerate 30 min, in logical N2In the case of, it is heated to
320 DEG C of min of stirring reaction 30.Put to room temperature, ethanol-n-hexane alternating centrifugal rinses multiple, obtained solid distributed and saved
In n-hexane.It can be seen that the Fe prepared from Fig. 1 (a)3O4Nanoparticle form is homogeneous, mostly spherical, is evenly distributed, grain
Footpath is about 15 ~ 20 nm.
The Fe of embodiment 23O4 @SiO2It is prepared by nanoparticle（SiO2Thickness degree is 12 nm）
Fe is prepared by reverse micelle of microemulsion3O4@SiO2Nanoparticle.2 mL Igepal CO-520 are dispersed in 42 mL hexamethylenes
In alkane, 30 min, the above-mentioned Fe of 10 mL are stirred3O4Cyclohexane solution (1 mg/mL) adds and is stirred continuously 3 h, adds ammoniacal liquor
325 μ L, 5 min are stirred, add the μ L of tetraethyl orthosilicate (TEOS) 150（Every 16 h adds 75 μ L）.Alcohol-water is handed over
For rinsing twice, preserve in ethanol.Gained Fe3O4@SiO2Nanoparticle form is homogeneous, globulate, SiO2Thickness degree is 12 nm.
The Fe of embodiment 33O4 @SiO2It is prepared by nanoparticle（SiO2Thickness degree is 16 nm）
Fe is prepared by reverse micelle of microemulsion3O4@SiO2Nanoparticle.2 mL Igepal CO-520 are dispersed in 42 mL hexamethylenes
In alkane, 30 min, the above-mentioned Fe of 10 mL are stirred3O4Cyclohexane solution (1mg/mL) adds and is stirred continuously 3 h, adds ammoniacal liquor 325
μ L, 5 min are stirred, add the μ L of tetraethyl orthosilicate (TEOS) 375（Every 16 h adds 75 μ L）.Alcohol-water alternately rushes
Wash 2 ~ 3 times, preserve in ethanol.Fig. 1（b）For gained Fe3O4@SiO2Nanoparticle, it can be seen that nanoparticle form is homogeneous, balling-up
Shape, SiO2Thickness degree is 16 nm.
The Fe of embodiment 43O4 @SiO2It is prepared by nanoparticle（SiO2Thickness degree is 25 nm）
Fe is prepared by reverse micelle of microemulsion3O4@SiO2Nanoparticle.2 mL Igepal CO-520 are dispersed in 42 mL hexamethylenes
In alkane, 30 min, the above-mentioned Fe of 10 mL are stirred3O4Cyclohexane solution (1mg/mL) adds and is stirred continuously 3 h, adds ammoniacal liquor 325
μ L, 5 min are stirred, add the μ L of tetraethyl orthosilicate (TEOS) 500（Every 16 h adds 75 μ L）.Alcohol-water alternately rushes
Wash 2 ~ 3 times, preserve in ethanol.Above-mentioned gained Fe3O4@SiO2The form of nanoparticle is spherical, disperses homogeneous, SiO2Thickness degree
For 25 nm.
Embodiment 5 builds Fe3O4@SiO2Three layers of temperature sensitive carriers of-p (NIPAM-co-NVP)
Above-mentioned three kinds of Fe3O4@SiO2Dispersion is carried out with 3- (trimethoxysilyl) propyl acrylate (MPS)
SiO2Surface modification, its ratio are 10 mL Fe3O4 @SiO2Ethanol solution (1 mg/mL) adds 100 μ L MPS, and stirring is anti-
After answering 4 h, alcohol flushing 2 ~ 3 times, it is stored in 2 mL ethanol.0.2 g NIPAs (PNIPAM) and 100
μ L NVPs (NVP) are dissolved in 25 mL water, add above-mentioned Fe3O4 @SiO2Solution, vacuum outgas 10
Min, 60 μ L N, N, N, N- tetramethylethylenediamines (TMEDA) are added, 0.6 mL ammonium persulfate solutions (10%, W/V), are led to
The min of nitrogen 15,70 DEG C of 4 h of waters bath with thermostatic control reaction after sealing.By product Magnetic Isolation, deionized water rinsing three times, it is stored in
In water.Fig. 1 (c) is based on SiO2The nm of thickness degree 16 Fe3O4@SiO2The three-decker particle Electronic Speculum that nanoparticle is prepared
Figure, temperature sensitive thickness is about 70 nm.
Embodiment 6 builds Fe3O4- p (NIPAM-co-NVP) chorion particle
Completed by silicon corrosion process：1 mL NaOH (1M) are added to the above-mentioned three kinds of different SiO of 4 mL2Thickness degree
In temperature sensitive particle solution, 6 ~ 8 h are stirred at room temperature, centrifugation, are washed 2 ~ 3 times.Fig. 1 (d) is based on SiO2The nm's of thickness degree 16
Temperature sensitive particle removes SiO2 Chorion particle electron microscope afterwards, chorion is clear in structure, and there is obvious cavity structure at center.Three kinds of different SiO2
The temperature sensitive particle of thickness degree removes SiO2 Afterwards gained chorion structure hollow thickness be respectively 12,16,22 nm.
Embodiment 7 loads adriamycin medicine
2 mL Fe3O4- p (NIPA-co-NVP) (1 mg/mL) chorion particle（Hollow thickness is 12 nm）The aqueous solution and 4
24 h of the mL adriamycins aqueous solution (1 mg/mL) mixing concussion.The drugloading rate for being computed resulting vehicle is 18%.
Embodiment 8 loads adriamycin medicine
2 mL Fe3O4- p (NIPA-co-NVP) (1 mg/mL) chorion particle（Hollow thickness is 16 nm）The aqueous solution and 4
24 h of the mL adriamycins aqueous solution (1 mg/mL) mixing concussion.The drugloading rate for being computed resulting vehicle is 22%.
Embodiment 9 loads adriamycin medicine
2 mL Fe3O4- p (NIPA-co-NVP) (1 mg/mL) chorion particle（Hollow thickness is 22 nm）The aqueous solution and 4
24 h of the mL adriamycins aqueous solution (1 mg/mL) mixing concussion.The drugloading rate for being computed resulting vehicle is 33%.
10 external heating curve of embodiment
Various concentrations carrier, in near-infrared laser (3 W/cm2) irradiation when, using thermal imaging system record temperature change,
Heating curve figure is made, described carrier concn is 200,100,50,20,10,5 μ g/mL；The irradiation time is 1,2,3,
4,5,6,7,8 min.
Fig. 2 is heating curve figure, and with the increase of near-infrared light application time, temperature constantly raises, and concentration is higher, and heating is faster.
The vitro drug release of embodiment 11
Near-infrared laser irradiates pharmaceutical carrier in 6 h internal clearances, investigates carrier in different pH value (pH 7.4 and pH
5.4) the insoluble drug release effect under.Fig. 3 is insoluble drug release design sketch, and under without near infrared light, medicine is in pH 5.4 and pH
Release is slow under 7.4 environment, increase NIR irradiations, and dashing forward, which occurs, in medicine releases effect, removes NIR, insoluble drug release slows down.
The targeting anti-tumor of embodiment 12
MCF-7 cells（Medical college of Jiangsu University provides）It is inoculated into after culture dish in 37 DEG C of incubators, 5% CO2Bar
24 h are incubated under part.One block of magnet is placed under culture dish, the carrier that medicine is loaded adds, after cultivating 6 h, through infrared laser（3
W/cm2）Irradiate 8 min.Cell is rinsed well carrier with PBS, Trypan Blue, observation by light microscope.Fig. 4 is that carrier exists
Under externally-applied magnetic field, magnetic target medicine therapeutic effect figure.1,2,3 is respectively the diverse location apart from magnet, it can be seen that distance
Magnet is nearer, and cell mortality is higher.
The carrier toxicity of embodiment 13 is tested
MCF-7 cells are inoculated into after 96 orifice plates in 37 DEG C of incubators, 5% CO2Under the conditions of incubate 24 h.It is separately added into not
With concentration adriamycin, the carrier of carrier and medicine loading, while investigate near-infrared laser induced drug releasing effect；Carrier is not
It is 10 ~ 500 μ g/mL with concentration；Described load drug carrier is calculated as 0.02 ~ 20 μ g/mL by doxorubicin concentration.
(3 W/cm under near-infrared laser irradiation2, 8 min), after being cultivated for 24 h, pass through the MTT methods of standard
Investigate toxic effect.Fig. 5 is MTT result figures, injury very little of the simple carrier to cell, with dense after NIR laser is given
Degree increase photo-thermal effect shows, to cell produce lethal effect, and carry drug carrier give NIR irradiation after, its toxicity to cell
It is also better than simple adriamycin effect, illustrate the photo-thermal therapy of carrier and injury that chemotherapy is combined to cell is bigger, be one
The good anti-tumor method of kind.
Claims (13)
1. a kind of preparation method with light-operated release function magnetic target medicine carrier, it is characterised in that enter according to following steps
OK：
（1）Fe3O4 Prepare： FeCl3·6H2O is soluble in water, adds ethanol, n-hexane, oleic acid, stirring
After add NaOH, stir 4 h after closed at 70 DEG C, separatory funnel separation solution, upper organic layer is rushed with deionized water
Wash repeatedly, evaporation of hexane is stayed overnight at 80 DEG C；Obtained product is dispersed in oleic acid and 1- octadecylene mixed solutions, N2Degassing
30 min, in logical N2In the case of, it is heated to 320 DEG C of min of stirring reaction 30；Put to room temperature, ethanol-n-hexane alternating centrifugal
Rinse repeatedly, obtained solid distributed and saved is in n-hexane；
（2）Fe3O4@SiO2It is prepared by nanoparticle：Fe is prepared by reverse micelle of microemulsion3O4@SiO2Nanoparticle,
Igepal CO-520 are dispersed in hexamethylene, stir 30 ~ 40 min, step（1）Obtained Fe3O4It is molten to be dissolved in hexamethylene
It is added to after liquid in above-mentioned solution and is stirred continuously 3 h, add ammoniacal liquor, is stirred 5 ~ 10 min, add tetraethyl orthosilicate
（TEOS）；Alcohol-water alternately rinses twice, preserves in ethanol；
（3）Build Fe3O4@SiO2Three layers of temperature sensitive carriers of-p (NIPAM-co-NVP)：
By 3- (trimethoxysilyl) propyl acrylate（MPS）It is added to step（2）Obtained Fe3O4 @SiO2Ethanol is molten
After the h of liquid stirring reaction 4, alcohol flushing 2 ~ 3 times, preserve in ethanol；NIPA (PNIPAM) and N- ethene
Base pyrrolidones (NVP) is soluble in water, adds the Fe of above-mentioned MPS modifications3O4@SiO2Ethanol solution, the min of vacuum outgas 10 ~ 20
, N, N, N are added, N- tetramethylethylenediamines (TMEDA), ammonium persulfate solution, leads to the min of nitrogen 15 ~ 20,70 °C after sealing
4 h are reacted in water bath with thermostatic control；By product Magnetic Isolation, deionized water rinsing 2 ~ 3 times, it is stored in water, obtains Fe3O4@SiO2-p
(NIPAM-co-NVP) solution；
（4）Build Fe3O4- p (NIPAM-co-NVP) chorion particle：
NaOH solution is added to Fe3O4@SiO2In-p (NIPAM-co-NVP) solution, 6 ~ 8 h, centrifugation, washing 2 are stirred at room temperature
~ 3 times.
2. a kind of preparation method with light-operated release function magnetic target medicine carrier according to claim 1, its feature
It is, step（2）Described in Igepal CO-520 and hexamethylene volume ratio be 1：21；Fe3O4The concentration of cyclohexane solution
For 1 mg/mL.
3. a kind of preparation method with light-operated release function magnetic target medicine carrier according to claim 1, its feature
It is, step（2）Described in Fe3O4The volume of cyclohexane solution, ammoniacal liquor and TEOS is 8 ~ 12 mL：325 µL：150~500 µ
L；Every 16 h of wherein TEOS add 75 μ L.
4. a kind of preparation method with light-operated release function magnetic target medicine carrier according to claim 3, its feature
It is, step（2）Described in Fe3O4The volume of cyclohexane solution, ammoniacal liquor and TEOS is 10 mL：325 µL：150~500 µL.
5. a kind of preparation method with light-operated release function magnetic target medicine carrier according to claim 1, its feature
It is, step（3）Described in Fe3O4 @SiO2Ethanol solution concentration is 1 mg/mL；
MPS and Fe3O4 @SiO2Volumes of aqueous ethanol ratio is 1: 100；
The ratio of PNIPAM, NVP and water is 1g：0.5 mL：125 mL；
TMEDA, ammonium persulfate solution volume ratio are 1：10, wherein ammonium persulfate solution mass volume ratio is 10%, and ammonium persulfate is molten
Liquid ratio of the total volume is 1% ~ 3%.
6. a kind of preparation method with light-operated release function magnetic target medicine carrier according to claim 5, its feature
It is, step（3）Described in ammonium persulfate solution ratio of the total volume be 2%.
7. a kind of preparation method with light-operated release function magnetic target medicine carrier according to claim 1, its feature
It is, step（4）Described in NaOH solution concentration be 0.8-1.5 M, NaOH solution and Fe3O4@SiO2-p(NIPAM-co-
NVP) volume ratio of solution is 1: 4.
8. a kind of preparation method with light-operated release function magnetic target medicine carrier according to claim 7, its feature
It is, step（4）Described in NaOH solution concentration be 1 M.
9. prepared by a kind of method as claimed in claim 1 has light-operated release function magnetic target medicine carrier.
A kind of 10. application with light-operated release function magnetic target medicine carrier load cancer therapy drug as claimed in claim 9.
A kind of 11. answering with light-operated release function magnetic target medicine carrier load cancer therapy drug as claimed in claim 10
With, it is characterised in that the cancer therapy drug is adriamycin.
A kind of 12. answering with light-operated release function magnetic target medicine carrier load cancer therapy drug as claimed in claim 11
With, it is characterised in that it is described application concrete operations be：By Fe3O4- p (NIPAM-co-NVP) the chorion particle aqueous solution and adriamycin
Aqueous solution mixing concussion.
A kind of 13. answering with light-operated release function magnetic target medicine carrier load cancer therapy drug as claimed in claim 12
With, it is characterised in that Fe3O4- p (NIPAM-co-NVP) chorion particle concentration of aqueous solution is 1 mg/mL；The adriamycin aqueous solution is dense
Spend for 1 mg/mL；The Fe3O4- p (NIPAM-co-NVP) the chorion particle aqueous solution is 1 with adriamycin aqueous solution volume ratio：4.
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| CN105498694B (en) * | 2015-11-30 | 2016-11-30 | 山东省分析测试中心 | The metallic organic framework magnetic material of a kind of temperature sensitive polymer parcel and application thereof |
| CN108079297B (en) * | 2018-01-16 | 2020-06-12 | 复旦大学 | Up-conversion luminescence-thermochemotherapy composite nanoprobe, preparation method thereof and application of combined therapy programmed control |
| CN109225175B (en) * | 2018-08-21 | 2021-07-20 | 江苏大学 | Magnetic composite microsphere and preparation method and application thereof |
| CN109939080B (en) * | 2019-03-11 | 2021-03-09 | 陕西师范大学 | Conjugated polymer-based thermosensitive drug-loaded nanoparticle and preparation method thereof |
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| Synthesis of NIPAAm-based polymer-grafted silica beads by;Park, Ki Chul et al.;《Reactive & Functional Polymers 》;20140403;第79卷;第36-49页 * |
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