CN104650052B - A kind of method that homo-orientin extract is prepared from the leaf of bamboo - Google Patents
A kind of method that homo-orientin extract is prepared from the leaf of bamboo Download PDFInfo
- Publication number
- CN104650052B CN104650052B CN201510078150.9A CN201510078150A CN104650052B CN 104650052 B CN104650052 B CN 104650052B CN 201510078150 A CN201510078150 A CN 201510078150A CN 104650052 B CN104650052 B CN 104650052B
- Authority
- CN
- China
- Prior art keywords
- orientin
- homo
- bamboo
- extract
- negative pressure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D407/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00
- C07D407/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings
- C07D407/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a kind of method that homo-orientin extract is prepared from the leaf of bamboo, it is characterised in that comprises the following steps：Step (1), Feedstock treating：The leaf of bamboo is cleaned, cleaned；Step (2), negative pressure extraction：The raw material of the step (1) processing is put into negative-pressure container, Extraction solvent is added, is filtered after 1 2h is extracted in the negative-pressure container and collect filtrate, concentrates, obtains homo-orientin extract of the present invention；The condition of negative pressure extraction is：Negative pressure is 0.065Mpa~0.075Mpa, 30~35 DEG C of temperature；The raw material and Extraction solvent are according to 1:10~1:15 (g/ml) solid-liquid ratio extraction.The present invention is extracted using negative pressure, solid-liquid ratio, condition of negative pressure and temperature condition and other factors when being extracted by controlling negative pressure, and the homo-orientin extract of high-purity is prepared.
Description
Technical field
It is more particularly to a kind of that homo-orientin extract is prepared from the leaf of bamboo the present invention relates to effective ingredients in plant extractive technique
Method.
Background technology
Homo-orientin (Isoorientin), also known as Lutonaretin, are the isomers of orientin, yellow crystal, molecule
Formula:C21H20O11, molecular weight:448.38, dissolve in the organic solvents such as methanol, ethanol, isopropanol, normal propyl alcohol, fusing point：230 DEG C~
232℃.Modern study shows that homo-orientin has significant protective effect to myocardial ischemia, anoxic.
The common extraction source of homo-orientin be Polygonum orientale (polygonaceae plant Polygonum knotweed (Polygonum orientale L.) it is dry
Zao Guo Miho and stem and branch with leaf)；We have discovered that Isoorientin content is typically more than 2% in bamboo-leaves flavones, and bamboo-leaves flavones
From the leaf of bamboo, extraction materials safety is cheap, and natural resources can be utilized more fully.
The content of the invention
The technical problem to be solved of the present invention is to provide a kind of method that homo-orientin extract is prepared from the leaf of bamboo,
The present invention is extracted using negative pressure, solid-liquid ratio, condition of negative pressure and temperature conditionss when being extracted by controlling negative pressure etc., height is prepared
The homo-orientin extract of purity.
Technical scheme is used by the present invention solves above-mentioned technical problem：
A kind of method that homo-orientin extract is prepared from the leaf of bamboo, comprises the following steps：
Step (1), Feedstock treating：The leaf of bamboo is cleaned, cleaned；
Step (2), negative pressure extraction：The raw material of the step (1) processing is put into negative-pressure container, adds Extraction solvent,
Filtered after extracting 1-2h in the negative-pressure container and collect filtrate, concentrated, obtain homo-orientin extract of the present invention；
The condition of negative pressure extraction is：Negative pressure is -0.065Mpa~-0.075Mpa, 30~35 DEG C of temperature；The raw material
With Extraction solvent according to 1:10~1:15 (g/ml) solid-liquid ratio extraction.
In preferable technical scheme, in the step (1), after the raw material is cleaned, following handle also is carried out：After cleaning
Leaf of bamboo crushed after being dried cross 20-60 mesh sieves.
In preferable technical scheme, in the step (2), the Extraction solvent is deionized water or pH value is 1.0-
3.0th, mass fraction is 65-85% ethanol solution.
In preferable technical scheme, in the step (2), the condition of the negative pressure extraction is：Negative pressure is -0.065Mpa,
35 DEG C of temperature；The Extraction solvent is that the ethanol solution is the ethanol solution that pH value is 2.0, mass fraction is 65%；It is described
Raw material and Extraction solvent are according to 1:10 (g/ml) solid-liquid ratio extraction.
In preferable technical scheme, in the step (2), the degree of the concentration is：Proportion 10~15 is concentrated into, is used
B measures.
The present invention compared with prior art, has the following advantages that：
(1) present invention is extracted using negative pressure, solid-liquid ratio, condition of negative pressure and temperature conditionss when being extracted by controlling negative pressure etc.
Factor, the homo-orientin extract of high-purity is prepared.In homo-orientin extract prepared by the present invention, homo-orientin purity
Up to 8%~10%.The purity refers to the mass content of the homo-orientin in homo-orientin extract.
(2) for the inventive method relative to prior art, Extracting temperature is lower, can be with as little as 30 DEG C, it can be ensured that extracted
Homo-orientin active component is not destroyed in journey.
Embodiment
Below by way of specific embodiment, the present invention is described in further detail.
Embodiment 1：
A kind of method that homo-orientin extract is prepared from the leaf of bamboo, comprises the following steps：
Step (1), Feedstock treating：The leaf of bamboo is removed into debris, cleaned；
Step (2), negative pressure extraction：By the step (1) processing the leaf of bamboo be put into negative-pressure container, add pH value be 2.0,
(leaf of bamboo and ethanol solution are according to 1 for the ethanol solution that mass fraction is 65%:10 (g/ml) solid-liquid ratio extraction, i.e., according to
The 1g leaf of bamboves add the ratio of 10ml ethanol solutions)；Filtered after extracting 2h in negative-pressure container and collect filtrate, be concentrated into proportion 10
(B measurement), obtains homo-orientin extract of the present invention.The condition of negative pressure extraction is：Negative pressure -0.065Mpa,
35 DEG C of temperature.
Determined through HPLC, in final homo-orientin extract, the purity of homo-orientin is 8.3%.
Embodiment 2：
A kind of method that homo-orientin extract is prepared from the leaf of bamboo, comprises the following steps：
Step (1), Feedstock treating：The leaf of bamboo is removed into debris, cleaned；Crushed after being dried crosses 50 mesh sieves, obtains bamboo leaf powder；
Step (2), negative pressure extraction：Into the bamboo leaf powder of the step (1) add pH value be 2.0, mass fraction 70%
Ethanol solution (bamboo leaf powder and ethanol solution are according to 1:10 (g/ml) solid-liquid ratio extraction, i.e., add according to 1g bamboo leaf powders
The ratio of 10ml ethanol solutions)；Filtered after extracting 1.5h in negative-pressure container and collect filtrate, concentrated, obtain of the present invention
Homo-orientin extract.The condition of negative pressure extraction is：Negative pressure -0.065Mpa, 30 DEG C of temperature.
Determined through HPLC, in final homo-orientin extract, the purity of homo-orientin is 11.8%.
Embodiment 3：
A kind of method that homo-orientin extract is prepared from the leaf of bamboo, comprises the following steps：
Step (1), Feedstock treating：The leaf of bamboo is removed into debris, cleaned；
Step (2), negative pressure extraction：Into the bamboo leaf powder of the step (1) add pH value be 2.0, mass fraction 75%
Ethanol solution (leaf of bamboo and ethanol solution are according to 1:12 (g/ml) solid-liquid ratio extraction, i.e., add 12ml according to the 1g leaf of bamboves
The ratio of ethanol solution)；Filtered after extracting 1.5h in negative-pressure container and collect filtrate, be concentrated into (the B of proportion 10
Measurement), obtain homo-orientin extract of the present invention.The condition of negative pressure extraction is：Negative pressure -0.075Mpa, temperature 30
℃。
Determined through HPLC, in final homo-orientin extract, the purity of homo-orientin is 8.5%.
Embodiment 4：
A kind of method that homo-orientin extract is prepared from the leaf of bamboo, comprises the following steps：
Step (1), Feedstock treating：The leaf of bamboo is removed into debris, cleaned；Crushed after being dried crosses 20 mesh sieves, obtains bamboo leaf powder；
Step (2), negative pressure extraction：Into the bamboo leaf powder of the step (1) add deionized water (bamboo leaf powder and go from
Sub- water is according to 1:15 (g/ml) solid-liquid ratio extraction, i.e., the ratio of 15ml deionized waters is added according to 1g bamboo leaf powders)；Hold in negative pressure
Filtered after extracting 1.5h in device and collect filtrate, concentrated, obtain homo-orientin extract of the present invention.The negative pressure extraction
Condition is：Negative pressure -0.065Mpa, 30 DEG C of temperature.
Determined through HPLC, in final homo-orientin extract, the purity of homo-orientin is 8.6%.
The eosinophilic's apoptosis-inducing of embodiment 5 is tested and the experiment of MBP kinase activities
Eosinophilic's apoptosis-inducing is tested:Experimental evidence Vermes etc. method (Journal of
Immunological Methods 39-51 pages of nineteen ninety-fives of volume 184) carry out.Eosinophilic cell HL-60 cells are made to suspend
In RPMI1640 culture mediums, cell concentration is 1-3 × 106Cell/ml.Add and be detected into the cell suspending liquid 500 μ l
Material (obtained homo-orientin extract is finally collected in above-described embodiment 1-4) or culture medium, make homo-orientin extract exist
Concentration in cell suspending liquid is 100 μ g/ml, in 37 DEG C, 5%CO2Under conditions of cultivate 6 hours or 24 hours.Centrifugation is cleaned
Afterwards, phosphatide phthalein associated proteins (Annexin) V buffer solutions are added, then add 5u1 phosphatide phthalein associated proteins V-FITC.Pass through stream
Dynamic hemacytometry, using phosphatide phthalein associated proteins V positive cells as apoptosis-inducing cell, with relative total cell number
Percentage is evaluated.
MBP Kinase activity assays:Experimental evidence De Souza etc. method (Blood 3432-3438 pages of volume 99 2002)
Carry out.Eosinophilic's cell HL-60 cells are suspended in RPMI1640 culture mediums, cell concentration is 1-3 × 106Cell/
ml.Tested substance or culture medium are added in the cell suspending liquid 500 μ l, it is 100 μ g/ml to make concentration, in 37 DEG C, 5%CO2's
Under the conditions of cultivate 6 hours or 24 hours.After centrifugation is cleaned, add and can dissolve buffer solution, cell is dissolved, using containing MBP 5mg/
Ml gel carries out electrophoresis.Gel is used after taking off denaturation, again denaturation treatment32P-ATP is marked, and it is anti-to implement the phosphorylation of 3 hours
Should.After gel drying, radioactivity is parsed using image analyzer.When 36kDa grows mark band, the work of MBP kinases is denoted as
Change positive (+).
Homo-orientin extract observed the apoptosis-inducing of concentration dependent in HL-60 cells.It is additionally carried out
During gel MBP kinase assays (In-gel MBP kinase assay), homo-orientin extract activation 36kDa MBP kinases.With
Upper result prompting.Homo-orientin extract is to eosinophils energy inducing cell apoptosis, the Apoptosis and 36kDa MBP kinases
It is relevant.Thus infer in homo-orientin extract of the present invention containing can be used for anti-eosinophilic's inflammatory effect medicine into
Point.
Acquired results are as shown in table 1 below:
Confirm that embodiment 2 has strong apoptosis-inducing activity, and with the activation of MBP kinases.
Table 1
As described above, can be preferably to realize the present invention.
Claims (1)
- A kind of 1. method that homo-orientin extract is prepared from the leaf of bamboo, it is characterised in that comprise the following steps：Step (1), Feedstock treating：The leaf of bamboo is removed into debris, cleaned；Crushed after being dried crosses 50 mesh sieves, obtains bamboo leaf powder；Step (2), negative pressure extraction：Into the bamboo leaf powder of the step (1) add pH value be 2.0, the second that mass fraction is 70% Alcoholic solution；Filtered after extracting 1.5h in negative-pressure container and collect filtrate, concentrated, obtain homo-orientin extract；The bamboo leaf powder and ethanol solution extract according to 1: 10 (g/ml) solid-liquid ratio, i.e., add 10ml ethanol according to 1g bamboo leaf powders The ratio of solution；The condition of negative pressure extraction is：Negative pressure -0.065Mpa, 30 DEG C of temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510078150.9A CN104650052B (en) | 2015-02-13 | 2015-02-13 | A kind of method that homo-orientin extract is prepared from the leaf of bamboo |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510078150.9A CN104650052B (en) | 2015-02-13 | 2015-02-13 | A kind of method that homo-orientin extract is prepared from the leaf of bamboo |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104650052A CN104650052A (en) | 2015-05-27 |
| CN104650052B true CN104650052B (en) | 2018-01-16 |
Family
ID=53241750
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510078150.9A Active CN104650052B (en) | 2015-02-13 | 2015-02-13 | A kind of method that homo-orientin extract is prepared from the leaf of bamboo |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104650052B (en) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008201725A (en) * | 2007-02-20 | 2008-09-04 | Univ Of Ryukyus | Anticaries agent |
| CN102285976A (en) * | 2011-09-27 | 2011-12-21 | 天津市尖峰天然产物研究开发有限公司 | Method for extracting isoorientin from bamboo leaf flavones |
| CN103263514B (en) * | 2013-05-23 | 2015-01-07 | 浙江省柑桔研究所 | Method for extracting flavones, low-molecule pectin and cellulose from orange peels in combined way |
| CN104587274B (en) * | 2015-02-13 | 2018-09-14 | 宁波杰顺生物科技有限公司 | A kind of leaf of bamboo homo-orientin extract |
-
2015
- 2015-02-13 CN CN201510078150.9A patent/CN104650052B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104650052A (en) | 2015-05-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109136174B (en) | Stem cell-derived exosome preparation for delaying senescence | |
| CN103215251B (en) | A kind of method being separated chloroplast DNA | |
| CN104531679A (en) | Method for extracting DNA from dry apricot leaf | |
| CN104587274B (en) | A kind of leaf of bamboo homo-orientin extract | |
| CN104650052B (en) | A kind of method that homo-orientin extract is prepared from the leaf of bamboo | |
| CN103772460B (en) | The extraction process of cordycepin in silkworm Chinese caterpillar fungus | |
| CN103767888B (en) | Sea grass polysaccharide is for preparing the application in cosmetics additive | |
| JP2013241355A (en) | Tie2 activator, angiogenesis inhibitor, blood vessel maturing agent, blood vessel normalizing agent, blood vessel stabilizer and pharmaceutical composition | |
| JP6995045B2 (en) | LPS extraction process | |
| CN104782657A (en) | Plant destructive distillation liquid, its preparation and its application in improvement of content of effective components in hairy root of Salvia miltiorrhiza | |
| CN108570101A (en) | The isolation and purification method of antibacterial peptide in a kind of apostichopus japonicus body-wall | |
| CN102807544B (en) | Method for extracting anthocyanin from elderberry fruits | |
| CN104186318B (en) | A kind of method that improves Sutellaria viscidula chromocor compound content | |
| CN107198668B (en) | Extract of antioxidant components in camellia seed hulls as well as extraction method and application thereof | |
| CN102125686B (en) | Application of hard clam polypeptide | |
| CN102784189A (en) | Cranberry extract | |
| CN113730950A (en) | Multistage circulating extraction equipment and preparation method for chlorella vulgaris and chlorella vulgaris deep purification factor | |
| CN102304177B (en) | Method for extracting and purifying lycoris radiata lectin from lycoris radiata bulbs and lycoris radiata lectin produced by method | |
| CN112402346A (en) | Acne-removing skin repairing composition and application thereof | |
| CN102772468B (en) | Elderberry anthocyanin extract | |
| CN103536725B (en) | A kind of preparation method of priceless health restoring powder and application | |
| Dev et al. | Antiprotozoal and anthelmintic naphthoquinones from three unexplored species of Diospyros | |
| CN114306122B (en) | Freckle-removing whitening component and application thereof in preparation of cosmetics | |
| Albrechtová et al. | The isolation of potato virus S from the leaves of potato plants using precipitation by polyethylene glycol | |
| CN106011097A (en) | Marchantia paleacea o-methyltransferase and coding gene and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |