CN104634953A - Environment-friendly and non-toxic hemolytic agent - Google Patents
Environment-friendly and non-toxic hemolytic agent Download PDFInfo
- Publication number
- CN104634953A CN104634953A CN201510090813.9A CN201510090813A CN104634953A CN 104634953 A CN104634953 A CN 104634953A CN 201510090813 A CN201510090813 A CN 201510090813A CN 104634953 A CN104634953 A CN 104634953A
- Authority
- CN
- China
- Prior art keywords
- parts
- hemolytic agent
- sodium
- toxic
- environment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the field of blood analysis, and particularly relates to an environment-friendly and non-toxic hemolytic agent which comprises a hemolytic agent A and a buffer solution, wherein the hemolytic agent A comprises 30-40 parts of sodium dodecyl sulfate, 2-4 parts of sodium ethylene diamine tetracetate, 2-5 parts of citric acid, 1-3 parts of sodium chloride and 60-80 parts of dimethyl carbonate; the buffer solution comprises 20 parts of sodium acetate and 50-65 parts of glacial acetic acid. The hemolytic agent is environmentally friendly and non-toxic, free from damage to a blood analysis instrument and high in accuracy.
Description
Technical field
The invention belongs to blood analysis field, be specifically related to a kind of Environment-friendlynon-toxic non-toxic hemolytic agent.
Background technology
Blood cell analysis is clinical the most frequently used laboratory parameters, obtains and applies rapidly and popularize.Need when using blood analysis device to carry out leukocyte differential count in blood cell analysis to use hemolytic agent, hemolytic agent has the ability destroying haemocyte structure, thus reaches the object of haemolysis, eliminates its interference to leukocyte analysis after hemolytic agent can destroy red blood cell.
Chinese patent CN1866011A discloses a kind of environment-friendly non-cyanogen hemolysis reagent, is damping fluid by sodium dihydrogen phosphate, the composition such as DTAC storage liquid, and this hemolytic agent utilizes water for solvent, dispersed poor and be three to hive off counting after haemolysis.
Summary of the invention
For prior art Problems existing, the invention provides a kind of Environment-friendlynon-toxic non-toxic hemolytic agent, after this hemolytic agent haemolysis, dispersion effect is good, and accuracy is high, is applicable to multiple five grouping blood analyser and uses, and to instrument not damaged, to the effect of human non-toxic's evil.
The technical scheme that the present invention is adopted to achieve these goals is:
The invention provides a kind of Environment-friendlynon-toxic non-toxic hemolytic agent, this hemolytic agent is made up of hemolytic agent A and damping fluid;
Described hemolytic agent A is made up of the component of following weight portion: sodium dodecylsulphonate 30-40 part, sodium ethylene diamine tetracetate 2-4 part, citric acid 2-5 part, sodium chloride 1-3 part, dimethyl carbonate 60-80 part; Described damping fluid is sodium acetate 20 parts, glacial acetic acid 50-65 part.
Further, optimization, hemolytic agent component A is: sodium dodecylsulphonate 35-38 part, sodium ethylene diamine tetracetate 3-4 part, citric acid 3-4 part, sodium chloride 2-3 part, dimethyl carbonate 75-78 part; The damping fluid optimized is sodium acetate 20 parts, 60 parts, glacial acetic acid.
In the present invention, by utilizing anionic surfactant sodium dodecylsulphonate, use amount is low but good to erythrocytic cracking ability, by suitable addition, reduces the ductility of film, and then causes cell seepage and haemolysis.The present invention adopts dimethyl carbonate as solvent, and can strengthen stability and the dispersiveness thereof of blood sample after haemolysis, meanwhile, dimethyl carbonate can strengthen the haemocylolysis of surfactant, complements each other, and reduces the running time.The present invention is by reasonably adding each component, and under hypotonic sour environment, hemolyzing effect is good but dialogue impact cell is little.
Advantage of the present invention and beneficial effect are: hemolytic agent asepsis environment-protecting provided by the invention, hemolyzing effect good and after haemolysis blood sample dispersiveness good, be convenient to subsequent operation, and to instrument not damaged, without the need to carrying out harmless treatment to waste liquid.
Embodiment
Instantiation mode below in conjunction with embodiment is described in further detail foregoing of the present invention again, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following embodiment.Without departing from the idea case in the present invention described above, the various replacement made according to ordinary skill knowledge and customary means or change, include within the scope of the invention.
Embodiment 1
Described hemolytic agent A is made up of the component of following weight portion: sodium dodecylsulphonate 35 parts, sodium ethylene diamine tetracetate 3 parts, citric acid 3 parts, 2 parts, sodium chloride, dimethyl carbonate 78 parts;
Described damping fluid is sodium acetate 20 parts, 60 parts, glacial acetic acid.
Preparation method: the dimethyl carbonate taking weight portion, then sodium dodecylsulphonate, sodium ethylene diamine tetracetate, the sodium chloride of weight portion, after dissolving completely, after adding citric acid, pH value is about 2.8, is prepared into hemolytic agent A; Take sodium acetate and the glacial acetic acid of weight portion, after mixing, obtain damping fluid.
Embodiment 2
Described hemolytic agent A is made up of the component of following weight portion: sodium dodecylsulphonate 30 parts, sodium ethylene diamine tetracetate 4 parts, citric acid 2 parts, 1.5 parts, sodium chloride, dimethyl carbonate 65 parts;
Described damping fluid is sodium acetate 20 parts, 55 parts, glacial acetic acid.
Preparation method: the dimethyl carbonate taking weight portion, then sodium dodecylsulphonate, sodium ethylene diamine tetracetate, the sodium chloride of weight portion, after dissolving completely, after adding citric acid, pH value is about 2.6, is prepared into hemolytic agent A; Take sodium acetate and the glacial acetic acid of weight portion, after mixing, obtain damping fluid.
Embodiment 3
Described hemolytic agent A is made up of the component of following weight portion: sodium dodecylsulphonate 38 parts, sodium ethylene diamine tetracetate 2 parts, citric acid 4.5 parts, 3 parts, sodium chloride, dimethyl carbonate 80 parts;
Described damping fluid is sodium acetate 20 parts, 50 parts, glacial acetic acid.
Preparation method: the dimethyl carbonate taking weight portion, then sodium dodecylsulphonate, sodium ethylene diamine tetracetate, the sodium chloride of weight portion, after dissolving completely, after adding citric acid, pH value is about 2.4, is prepared into hemolytic agent A; Take sodium acetate and the glacial acetic acid of weight portion, after mixing, obtain damping fluid.
Hemolytic agent using method of the present invention: hemolytic agent A is joined after in blood sample to be measured, add damping fluid again every a period of time.
Comparative analysis: if substituted by solvent dimethyl carbonate distilled water in embodiment 1, then after adding solvent orange 2 A, blood sample bad dispersibility, easily clustering phenomena occurs, impact analysis result.
Effect example
Randomly draw clinical fresh blood sample 10 parts, the hemolytic agent adopting embodiment 1 to prepare carries out haemolysis respectively with existing standard haemolytic agent and carry out Arneth's count on Beckman LH750 automatic blood analyzer, and testing result is in table 1.
Table 1
As can be seen from Table 1, the leukocyte differential count result that the present invention detects is compared with standard haemolytic agent, meets the standard-required of People's Republic of China's pharmaceuticals industry standard about hemolytic agent, within its critical field.After hemolytic agent haemolysis prepared by the present invention, dispersion effect is good, and testing result accuracy is high, asepsis environment-protecting, improves working environment and to blood analysis instrument not damaged.
Claims (2)
1. an Environment-friendlynon-toxic non-toxic hemolytic agent, is characterized in that: this hemolytic agent is made up of hemolytic agent A and damping fluid;
Described hemolytic agent A is made up of the component of following weight portion: sodium dodecylsulphonate 30-40 part, sodium ethylene diamine tetracetate 2-4 part, citric acid 2-5 part, sodium chloride 1-3 part, dimethyl carbonate 60-80 part;
Described damping fluid is sodium acetate 20 parts, glacial acetic acid 50-65 part.
2. Environment-friendlynon-toxic non-toxic hemolytic agent according to claim 1, is characterized in that, described hemolytic agent component A is: sodium dodecylsulphonate 35-38 part, sodium ethylene diamine tetracetate 3-4 part, citric acid 3-4 part, sodium chloride 2-3 part, dimethyl carbonate 75-78 part;
Described damping fluid is sodium acetate 20 parts, 60 parts, glacial acetic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510090813.9A CN104634953A (en) | 2015-02-28 | 2015-02-28 | Environment-friendly and non-toxic hemolytic agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510090813.9A CN104634953A (en) | 2015-02-28 | 2015-02-28 | Environment-friendly and non-toxic hemolytic agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104634953A true CN104634953A (en) | 2015-05-20 |
Family
ID=53213934
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510090813.9A Pending CN104634953A (en) | 2015-02-28 | 2015-02-28 | Environment-friendly and non-toxic hemolytic agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104634953A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4853338A (en) * | 1987-05-20 | 1989-08-01 | Technicon Instruments Corporation | Cyanide-free hemoglobin reagent |
| EP0444241B1 (en) * | 1990-03-01 | 1995-01-11 | Toa Medical Electronics Co., Ltd. | Reagent for measurement of leukocytes and hemoglobin in blood |
| CN1866011A (en) * | 2006-05-18 | 2006-11-22 | 刘爱兵 | Environment-friendly non-cyanogen hemolysis reagent |
| CN101236192A (en) * | 2007-01-29 | 2008-08-06 | 深圳迈瑞生物医疗电子股份有限公司 | Hemolytic agent, leucocyte classification reagent system and classification method |
| CN101329229A (en) * | 2008-07-30 | 2008-12-24 | 山东兰桥医学科技有限公司 | Cyanogen-free leucocyte tri-grouping environment protection type haemolysin for blood cell analysis |
| CN102226804A (en) * | 2011-03-28 | 2011-10-26 | 中国人民解放军总医院 | Hemolytic agent for blood leukocyte five-classification counting and application thereof |
| CN102662067A (en) * | 2012-05-20 | 2012-09-12 | 烟台卓越生物技术有限责任公司 | Cyanide-free hemolysin for determining hemoglobin concentration and carrying out white blood cell count by groups |
| CN103323582A (en) * | 2013-06-18 | 2013-09-25 | 南京普朗医疗设备有限公司 | Leukocyte classification hemolytic agent and kit thereof |
| CN103698501A (en) * | 2013-12-23 | 2014-04-02 | 深圳市开立科技有限公司 | Cyanide-free hemolytic agent |
-
2015
- 2015-02-28 CN CN201510090813.9A patent/CN104634953A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4853338A (en) * | 1987-05-20 | 1989-08-01 | Technicon Instruments Corporation | Cyanide-free hemoglobin reagent |
| EP0444241B1 (en) * | 1990-03-01 | 1995-01-11 | Toa Medical Electronics Co., Ltd. | Reagent for measurement of leukocytes and hemoglobin in blood |
| CN1866011A (en) * | 2006-05-18 | 2006-11-22 | 刘爱兵 | Environment-friendly non-cyanogen hemolysis reagent |
| CN101236192A (en) * | 2007-01-29 | 2008-08-06 | 深圳迈瑞生物医疗电子股份有限公司 | Hemolytic agent, leucocyte classification reagent system and classification method |
| CN101329229A (en) * | 2008-07-30 | 2008-12-24 | 山东兰桥医学科技有限公司 | Cyanogen-free leucocyte tri-grouping environment protection type haemolysin for blood cell analysis |
| CN102226804A (en) * | 2011-03-28 | 2011-10-26 | 中国人民解放军总医院 | Hemolytic agent for blood leukocyte five-classification counting and application thereof |
| CN102662067A (en) * | 2012-05-20 | 2012-09-12 | 烟台卓越生物技术有限责任公司 | Cyanide-free hemolysin for determining hemoglobin concentration and carrying out white blood cell count by groups |
| CN103323582A (en) * | 2013-06-18 | 2013-09-25 | 南京普朗医疗设备有限公司 | Leukocyte classification hemolytic agent and kit thereof |
| CN103698501A (en) * | 2013-12-23 | 2014-04-02 | 深圳市开立科技有限公司 | Cyanide-free hemolytic agent |
Non-Patent Citations (1)
| Title |
|---|
| 洪仲苓: "《化工有机原料深加工》", 30 June 1997, 化学工业出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102226804B (en) | Hemolytic agent for blood leukocyte five-classification counting and application thereof | |
| CN103323582B (en) | Leukocyte classification hemolytic agent and kit thereof | |
| CN102113481B (en) | Exfoliated cell preservation solution and preparation method thereof | |
| CN1018586B (en) | Method and reagent system for isolation identification and/or analysis of leukocytes from whole blood samples | |
| CN103789202A (en) | Container for collecting nucleic acid preserved at normal temperature | |
| CN105116141B (en) | A kind of one-component TMB nitrite ion and preparation method thereof | |
| CN104041484B (en) | A kind of cell-preservation liquid | |
| CN106442078B (en) | A kind of alkalinity liquid basal cell saves treatment fluid and preparation method thereof | |
| CN104359906B (en) | A kind of stabilization, the serum tolal bile acid detection reagent of strong antijamming capability | |
| CN103336110A (en) | Whole blood quality control material and preparation method thereof | |
| CN103323308A (en) | Reagent for processing trace whole blood and application thereof | |
| CN104404127A (en) | Blood alanine aminotransferase detecting reagent with high stability | |
| CN102662067A (en) | Cyanide-free hemolysin for determining hemoglobin concentration and carrying out white blood cell count by groups | |
| CN105738462A (en) | Detection method for microelements in peripheral blood | |
| CN108872225A (en) | A kind of detection reagent and detection method detecting animal blood cell | |
| CN104634953A (en) | Environment-friendly and non-toxic hemolytic agent | |
| CN105486682A (en) | Rapid protein detection kit as well as detection method and application using same | |
| CN108318408A (en) | The pretreating reagent and method of leukocyte differential count sample | |
| CN104655542A (en) | Synthetic blood for detecting protective products, and preparation method of synthetic blood | |
| CN104698159B (en) | A kind of detection method of endotoxin content | |
| CN104237188A (en) | Fluorescent probe for simply and quickly detecting zinc ions | |
| CN103217525A (en) | Composition for improving cystatin C latex coated antibody stability, stabilizer containing the same, preparation method and application thereof | |
| CN108169468A (en) | A kind of dilution and its configuration method suitable for a variety of blood analysers | |
| CN104729905A (en) | Anticoagulant, preparing method of anticoagulant and method of preparing blood smear with same | |
| CN107266522A (en) | A kind of universal gross protein extracts reagent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150520 |
|
| RJ01 | Rejection of invention patent application after publication |