CN104630196A - Method for producing low-temperature gamma-lactamase by virtue of microbial fermentation - Google Patents
Method for producing low-temperature gamma-lactamase by virtue of microbial fermentation Download PDFInfo
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Abstract
The invention relates to a method for producing a low-temperature gamma-lactamase by virtue of microbial fermentation. The method comprises the following specific steps: firstly activating a strain capable of producing gamma-lactamase, carrying out step-by-step low-temperature acclimation so that the strain well grows in a low-temperature environment, carrying out step-by-step enlarge cultivation on strain capable of producing gamma-lactamase and subjected to low-temperature acclimation at 10-16 DEG C, inoculating the strain subjected to enlarge cultivation onto a liquid fermentation culture medium according to an inoculation amount which accounts for 3-9% of the volume of a fermentation broth, culturing for 72-144 hours at 10-16 DEG C to obtain the low-temperature gamma-lactamase, and further concentrating, separating and purifying the collected crude enzyme solution according to different needs and different objects to obtain enzyme preparations with different activities, purities and dosages. The low-temperature gamma-lactamase produced by the method is high in enzyme activity and catalytic efficiency at low temperature, the processing process can be reduced, the processing time is shortened, the production cost is decreased, the production efficiency is increased, and the product quality is increased and improved; and the enzyme preparations are simple in operation and application, are convenient and rapid and have broader industrial development value and market application advantages.
Description
Technical field
The present invention relates to the fields such as microbiology, enzyme engineering, fermentation engineering, biological chemistry, be specifically related to a kind of method that fermentable produces low temperature gamma-lactam enzyme.
Background technology
Gamma-lactam enzyme (γ-lactamses, EC 3.5.2.-) is the novel Ntn hydrolase of one of catalysis amides amido linkage hydrolysis.(+) gamma-lactam enzyme can high-level efficiency kinetic resolution of racemic body gamma-lactam, obtains optically pure (-) gamma-lactam.Optically pure (-) gamma-lactam is the chiral intermediate of preparation antiviral, and being the synthesis material of anti-AIDS drug (-) Abacavir and (-) Carbovir, is also the synthesis material of Tamiflu Peramivir.In addition, gamma-lactam enzyme catalysis racemic modification gamma-lactam also can obtain the gamma-aminobutyric acid analog (GABA) of different configuration.GABA is a kind of inhibitory neurotransmitter, and it participates in multiple Metabolic activity, has very high physiologically active [1].People are before discovery (+) gamma-lactam enzyme, the method of general employing asymmetric synthesis prepares optically pure (-) gamma-lactam, the shortcoming of this method is expensive starting materials, technique is loaded down with trivial details, production cost is high, cause environmental pollution, is unfavorable for suitability for industrialized production [2].Use gamma-lactam enzyme to carry out suitability for industrialized production (-) gamma-lactam, there is reaction conditions gentleness, technique is simple, production cost is low, environmental friendliness, product optical purity advantages of higher.At present be in the research of fermentable production gamma-lactam enzyme seed selection and optimization culture conditions [3 ~ 5], zymologic property and the Cloning of Genes Related stage [6 ~ 8] that gamma-lactam enzyme produces bacterial classification, and mainly concentrate on middle temperature gamma-lactam enzyme, yet there are no the correlative study report that fermentable produces low temperature gamma-lactam enzyme at present both at home and abroad, fermentable is produced low temperature gamma-lactam enzyme and is had wide potentiality to be exploited and application prospect.
Reference:
[1] Wang Jianjun, Zheng Guojun, Wu Sheng. the progress of microbe-derived gamma-lactam enzyme. microorganism journal, 2010;
[2]Velazquez F.,Olivo HF.The application ofchiraloxazolidinethiones and thiazolidine-thioned in asymmetric synthesis.Current Organic Chemistry,2002；
[3]Taylor S J C,Sutherland A G,Lee C,et al.Chemoenzymatic synthesis of(-)-carbovir utilizing a whole cell catalysed resolution of 2-azabicyclo[2.2.1]hept-5-en-3-one[J].Journal ofthe Chemical Society：Chemical Communications,1990；
[4] Li Haiquan, Su Lei, willow, etc. produce screening and the Fermentation Conditions of gamma-lactam hydrolytic enzyme strain. microorganism journal, 2006;
[5] Chen Honggan, Ni Ye, Sun Zhihao. high yield (+) gamma-lactam enzyme bacteria selection and enzymatic production Study of China biotechnology magazine, 2012;
[6]Janjun Wang,Xiaoyan Guo,Guojun Zheng.Purification and characterization of an novel(-)gamma-lactamase from Microbacterium hydrocarbonoxydans Annals ofMicrobiology,2009；
[7]Xiaoxi Qin,Jianjun Wang,Guojun Zheng,et al.Enantioselective Resolution ofγ-Lactam by a Whole Cell of Microbacterium hydrocarbonoxydans(L29-9)Immobilized in Polymer of PVA-Alginate-Boric Acid[J].Appl Biochem Biotechnol,2010；
[8]Yang M,Gao Q,Wu S,et al.Characterization of a recombinant(-)γ-lactamase from Microbacterium hydrocarbonoxydans.Biotechnology Letters,2012。
Summary of the invention
The object of this invention is to provide a kind of method that fermentable produces low temperature gamma-lactam enzyme, the method can produce gamma-lactam enzyme bacterial classification first to activate, again through domestication by low temperature, finally at 10 ~ 16 DEG C, liquid fermenting produces low temperature gamma-lactam enzyme, the low temperature gamma-lactam enzyme that this production method obtains, through abstraction and purification, can obtain the zymin of different concns and purity.The method that a kind of fermentable of the present invention produces low temperature gamma-lactam enzyme specifically comprises the following steps:
(1) gamma-lactam enzyme bacterial classification can be produced first activate, domestication by low temperature step by step again, acclimation temperature from high to low, 30 DEG C → 25 DEG C → 22 DEG C → 20 DEG C → 18 DEG C → 16 DEG C → 14 DEG C → 12 DEG C → 10 DEG C, when namely bacterial classification stable growth in low temperature environment tames end; The domestication by low temperature substratum of this bacterial classification consists of: yeast extract paste 0.2 ~ 5.0g, N-acetyl-L-Phe 1.0 ~ 10.0g, NH
4cl 2.0 ~ 15.0g, Na
2hPO
40.1 ~ 1.0g, NaH
2pO
40.1 ~ 1.0g, MgSO
40.05 ~ 0.5g, agar powder 20g, tap water 1.0L, pH are 6.0 ~ 8.0, in 103kpa, 121 DEG C of high pressure steam sterilization 30min;
The described gamma-lactam enzyme bacterial classification that can produce derives from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), bacterium numbering: 1.219 or 1.758 or 1.1830;
(2) according to a conventional method by the gamma-lactam enzyme producing strains after domestication by low temperature in step (1) in liquid seed culture medium in 10 ~ 16 DEG C of enlarged culturing step by step, be prepared into liquid first order seed and secondary seed; Described liquid seed culture medium: yeast extract paste 20.0 ~ 25.0g, peptone 10.0 ~ 20g, NaCl 10.0 ~ 15.0g, MgSO
40.05 ~ 0.5g, ZnSO
47H
2o 2.4 ~ 8.0mg, tap water 1.0L, pH are 6.0 ~ 8.0, in 103kpa, 121 DEG C of high pressure steam sterilization 30min;
(3) pressed by liquid two stage seed in 3 ~ 9% inoculum size access liquid culture mediums of fermentating liquid volume, when cultivating 72 ~ 144h for 10 ~ 16 DEG C, namely fermentable production low temperature gamma-lactam enzyme terminates; Described liquid culture medium: extractum carnis 10.0 ~ 25.0g, peptone 2.0 ~ 20.0g, ethanamide 5.0 ~ 20g, Tween80 0.1 ~ 0.4g, (NH
4)
2sO
41.0 ~ 1.5g, KH
2pO
41.4 ~ 2.0g, MgSO
40.1 ~ 0.4g, CaCl
20.2 ~ 0.3g, ZnSO
47H
2o 2.4 ~ 8.0mg, tap water 1.0L, pH are 5.0 ~ 8.0, in 103kpa, 121 DEG C of high pressure steam sterilization 30min;
(4) by the fermented liquid of step (3) at 4000 ~ 8000rpm collected by centrifugation thalline, ultrasonication, the supernatant liquor collected is crude enzyme liquid;
(5) different with use object according to difference needs, the crude enzyme liquid that step (4) can also be obtained is concentrated, separation and purification further, is prepared into the zymin of different activities, purity and formulation.
Further, the activation medium that step (1) produces gamma-lactam enzyme bacterial classification is: yeast extract paste 20.0g, peptone 10.0g, NaCl10.0g, agar 20.0g, tap water 1.0L, pH7.4, in 103kpa, and 121 DEG C of high pressure steam sterilization 30min.
The explanation that can produce gamma-lactam enzyme bacterial classification initial activation and growth conditions in the present invention provides by China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) is carried out.Bacterial classification first activates, produce low temperature gamma-lactam enzyme through domestication by low temperature secondary fermentation step by step again cultivates by condition of enzyme production of the present invention, bacterial classification after domestication by low temperature can preserve 2 months in 4 DEG C of environment, the bacteria suspension made with 10 ~ 25vt% glycerine, can long term storage under-80 DEG C of conditions.
Compared with prior art, the invention has the beneficial effects as follows:
(1) the low temperature gamma-lactam enzyme that the present invention produces can make the vigor of cold-adapted enzyme lose through gentle thermal treatment, and low temperature or thermophilic process can't affect the quality of product, also have high enzymatic activity and high catalytic efficiency especially at low temperatures; Improve production efficiency, improve and improve the quality of products;
(2) method of the present invention simplifies complete processing, shorten process period and fundamentally avoid the heating of middle temperature, high temperature enzyme, the link of cooling apparatus, save expensive heating or refrigeration costs, reduce production cost, in energy-conservation, have sizable advantage;
(3) the low temperature gamma-lactam enzyme utilizing fermentable to produce is mainly used in resolution of racemates gamma-lactam, obtain optically pure (-) gamma-lactam, the application of low temperature gamma-lactam enzyme can further improve utilization ratio and the transformation efficiency of raw material, and this zymin in the application simple, convenient, quick, cost is low, has more wide commercial exploitation value and market application advantage.
Embodiment
Below by embodiment, the present invention will be further described, but do not limit the present invention.
Embodiment 1
(1) medium preparing
1. strain activation and culture base: yeast extract paste 20.0g, peptone 10.0g, NaCl10.0g, agar 20.0g, tap water 1.0L, pH7.4, in 103kpa, 121 DEG C of high pressure steam sterilization 30min;
2. bacterial classification domestication by low temperature substratum: yeast extract paste 0.2g, N-acetyl-L-Phe 1.0g, NH
4cl 2.0g, Na
2hPO4 0.1g, NaH
2pO4 0.1g, MgSO
40.05g, agar powder 20g, tap water 1.0L, pH value is 6.0, in 103kpa, 121 DEG C of high pressure steam sterilization 30min;
3. liquid seed culture medium: yeast extract paste 20.0g, peptone 10.0g, NaCl 10.0g, MgSO
40.05g; ZnSO
47H
2o 2.4mg, tap water 1.0L, pH6.0, in 103kpa, 121 DEG C of high pressure steam sterilization 30min;
4. liquid culture medium: extractum carnis 10.0g, peptone 2.0g, ethanamide 5.0g, Tween80 0.1g, (NH
4)
2sO
41.0g, KH
2pO
41.4g, MgSO
40.1g, CaCl
20.2g, ZnSO
47H
2o 2.4mg, tap water 1.0L, pH value 5.0,121 DEG C, in 103kpa, 121 DEG C of high pressure steam sterilization 30min.
(2) will produce the bacterial classification of gamma-lactam enzyme, the bacterial classification provided by China General Microbiological culture presevation administrative center (CGMCC) illustrates and carries out initial activation;
(3) by the bacterial classification that activated in (2) domestication by low temperature step by step in domestication by low temperature substratum, acclimation temperature from high to low, 30 DEG C → 25 DEG C → 22 DEG C → 20 DEG C → 18 DEG C → 16 DEG C → 14 DEG C → 12 DEG C → 10 DEG C, tame about 8 days, make bacterial classification can in low temperature environment well-grown, when namely bacterial classification stable growth in low temperature environment tames end;
(4) carry out enlarged culturing step by step by the inoculum size of 5vt% after according to a conventional method the gamma-lactam enzyme producing strains after domestication by low temperature in step (3) being cultivated 48 ~ 72h in 10 ~ 12 DEG C in liquid seed culture medium, be prepared into liquid first order seed and secondary seed;
(5) pressed by the secondary seed of preparation in (4) in the liquid culture medium of inoculum size access 5L of fermentating liquid volume 3 ~ 5%, when cultivating 120 ~ 144h for 10 ~ 12 DEG C, namely fermentable production low temperature gamma-lactam enzyme terminates;
(6) fermented liquid of step (5) is collected liquid at the centrifugal 20min of 4000 ~ 8000rpm, the supernatant liquor collected is crude enzyme liquid;
(7) different with use object according to difference needs, the crude enzyme liquid that (6) can also be obtained is concentrated, separation and purification further, is prepared into the low temperature gamma-lactam zymin of different activities, purity and formulation.
Embodiment 2
(1) medium preparing
1. strain activation and culture base: yeast extract paste 20.0g, peptone 10.0g, NaCl10.0g, agar 20.0g, tap water 1.0L, pH7.4,121 DEG C, 103kpa, high pressure steam sterilization 30min.
2. bacterial classification domestication by low temperature substratum: yeast extract paste 2.0g; N-acetyl-L-Phe 6.0g; NH
4cl 6.0g; Na
2hPO
40.5g; NaH
2pO
40.5g; MgSO
40.05g; Agar powder 20g, tap water 1.0L, pH value is 7.0,121 DEG C, 103kpa, high pressure steam sterilization 30min.
3. liquid seed culture medium: yeast extract paste 20.0g, peptone 15.0g, NaCl12.0g, MgSO
40.01g; ZnSO
47H
2o 6.0mg, tap water 1.0L, pH7.0,121 DEG C, 103kpa, high pressure steam sterilization 30min.
4. liquid culture medium: extractum carnis 15.0g, peptone 15.0g, ethanamide 15.0g, Tween80 0.3g, (NH
4)
2sO
41.2g, KH
2pO
41.6g, MgSO
40.3g, CaCl
20.2g, ZnSO
47H
2o 6.0mg, tap water 1.0L, pH value 6.0,121 DEG C, 103kpa, high pressure steam sterilization 30min.
(2) will produce the bacterial classification of gamma-lactam enzyme, the bacterial classification provided by China General Microbiological culture presevation administrative center (CGMCC) illustrates and carries out initial activation;
(3) bacterial classification (2) activated domestication by low temperature step by step in domestication by low temperature substratum, acclimation temperature from high to low, 30 DEG C → 25 DEG C → 22 DEG C → 20 DEG C → 18 DEG C → 16 DEG C → 14 DEG C → 12 DEG C → 10 DEG C, tame about 8 days, make bacterial classification can in low temperature environment well-grown, when namely bacterial classification stable growth in low temperature environment tames end;
(4) carry out enlarged culturing step by step by the inoculum size of 5vt% after according to a conventional method the gamma-lactam enzyme producing strains after domestication by low temperature in step (3) being cultivated 48 ~ 72h in 12 ~ 14 DEG C in liquid seed culture medium, be prepared into liquid first order seed and secondary seed;
(5) pressed by the secondary seed of preparation in (4) in the liquid culture medium of inoculum size access 50L of fermentating liquid volume 5 ~ 7%, when cultivating 120h for 12 ~ 14 DEG C, namely fermentable production low temperature gamma-lactam enzyme terminates;
(6) fermented liquid of step (5) is collected liquid at the centrifugal 20min of 4000 ~ 8000rpm, the supernatant liquor collected is crude enzyme liquid;
(7) different with use object according to difference needs, the crude enzyme liquid that (6) can also be obtained is concentrated, separation and purification further, is prepared into the low temperature gamma-lactam zymin of different activities, purity and formulation.
Embodiment 3
(1) medium preparing
1. strain activation and culture base: yeast extract paste 20.0g, peptone 10.0g, NaCl10.0g, agar 20.0g, tap water 1.0L, pH7.4,121 DEG C, 103kpa, high pressure steam sterilization 30min.
2. bacterial classification domestication by low temperature substratum: yeast extract paste 5.0g; N-acetyl-L-Phe 10.0g; NH
4cl 15.0g; Na
2hPO
41.0g; NaH
2pO
41.0g; MgSO
40.5g; Agar powder 20g, tap water 1.0L, pH value is 8.0,121 DEG C, 103kpa, high pressure steam sterilization 30min.
3. liquid seed culture medium: yeast extract paste 25.0g, peptone 20g, NaCl 15.0g, MgSO
40.5g; ZnSO
47H
2o 8.0mg, tap water 1.0L, pH8.0,121 DEG C, 103kpa, high pressure steam sterilization 30min.
4. liquid culture medium: extractum carnis 25.0g, peptone 20.0g, ethanamide 20g, Tween80 0.4g, (NH
4)
2sO
41.5g, KH
2pO
42.0g, MgSO
40.4g, CaCl
20.3g, ZnSO
47H
2o 8.0mg, tap water 1.0L, pH value 8.0,121 DEG C, 103kpa, high pressure steam sterilization 30min.
(2) will produce the bacterial classification of gamma-lactam enzyme, the bacterial classification provided by China General Microbiological culture presevation administrative center (CGMCC) illustrates and carries out initial activation;
(3) bacterial classification (2) activated domestication by low temperature step by step in domestication by low temperature substratum, acclimation temperature from high to low, 30 DEG C → 25 DEG C → 22 DEG C → 20 DEG C → 18 DEG C → 16 DEG C → 14 DEG C → 12 DEG C → 10 DEG C, tame about 8 days, make bacterial classification can in low temperature environment well-grown, when namely bacterial classification stable growth in low temperature environment tames end;
(4) carry out enlarged culturing step by step by the inoculum size of 5vt% after according to a conventional method the gamma-lactam enzyme producing strains after domestication by low temperature in step (3) being cultivated 48 ~ 72h in 14 ~ 16 DEG C in liquid seed culture medium, be prepared into liquid first order seed and secondary seed;
(5) pressed by the secondary seed of preparation in (4) in the liquid culture medium of inoculum size access 500L of fermentating liquid volume 7 ~ 9%, when cultivating 72h for 14 ~ 16 DEG C, namely fermentable production low temperature gamma-lactam enzyme terminates;
(6) fermented liquid of step (5) is collected liquid at the centrifugal 20min of 4000 ~ 8000rpm, the supernatant liquor collected is crude enzyme liquid.
(7) different with use object according to difference needs, the crude enzyme liquid that (6) can also be obtained is concentrated, separation and purification further, is prepared into the low temperature gamma-lactam zymin of different activities, purity and formulation.
Claims (2)
1. fermentable produces a method for low temperature gamma-lactam enzyme, it is characterized in that, comprises the following steps:
(1) gamma-lactam enzyme bacterial classification can be produced first activate, domestication by low temperature step by step again, acclimation temperature from high to low, 30 DEG C → 25 DEG C → 22 DEG C → 20 DEG C → 18 DEG C → 16 DEG C → 14 DEG C → 12 DEG C → 10 DEG C, when namely bacterial classification stable growth in low temperature environment tames end; The domestication by low temperature substratum of this bacterial classification consists of: yeast extract paste 0.2 ~ 5.0g, N-acetyl-L-Phe 1.0 ~ 10.0g, NH
4cl2.0 ~ 15.0g, Na
2hPO
40.1 ~ 1.0g, NaH
2pO
40.1 ~ 1.0g, MgSO
40.05 ~ 0.5g, agar powder 20g, tap water 1.0L, pH are 6.0 ~ 8.0, in 103kpa, 121 DEG C of high pressure steam sterilization 30min;
The described gamma-lactam enzyme bacterial classification that can produce derives from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), bacterium numbering: 1.219 or 1.758 or 1.1830;
(2) according to a conventional method by the gamma-lactam enzyme producing strains after domestication by low temperature in step (1) in liquid seed culture medium in 10 ~ 16 DEG C of enlarged culturing step by step, be prepared into liquid first order seed and secondary seed; Described liquid seed culture medium: yeast extract paste 20.0 ~ 25.0g, peptone 10.0 ~ 20g, NaCl 10.0 ~ 15.0g, MgSO
40.05 ~ 0.5g, ZnSO
47H
2o 2.4 ~ 8.0mg, tap water 1.0L, pH are 6.0 ~ 8.0, in 103kpa, 121 DEG C of high pressure steam sterilization 30min;
(3) pressed by liquid two stage seed in 3 ~ 9% inoculum size access liquid culture mediums of fermentating liquid volume, when cultivating 72 ~ 144h for 10 ~ 16 DEG C, namely fermentable production low temperature gamma-lactam enzyme terminates; Described liquid culture medium: extractum carnis 10.0 ~ 25.0g, peptone 2.0 ~ 20.0g, ethanamide 5.0 ~ 20g, Tween800.1 ~ 0.4g, (NH
4)
2sO
41.0 ~ 1.5g, KH
2pO
41.4 ~ 2.0g, MgSO
40.1 ~ 0.4g, CaCl
20.2 ~ 0.3g, ZnSO
47H
2o 2.4 ~ 8.0mg, tap water 1.0L, pH are 5.0 ~ 8.0, in 103kpa, 121 DEG C of high pressure steam sterilization 30min;
(4) by the fermented liquid of step (3) at 4000 ~ 8000rpm collected by centrifugation thalline, ultrasonication, the supernatant liquor collected is crude enzyme liquid;
(5) different with use object according to difference needs, the crude enzyme liquid that step (4) can also be obtained is concentrated, separation and purification further, is prepared into the zymin of different activities, purity and formulation.
2. a kind of fermentable according to claim 1 produces the method for low temperature gamma-lactam enzyme, it is characterized in that, the activation medium that step (1) produces gamma-lactam enzyme bacterial classification is: yeast extract paste 20.0g, peptone 10.0g, NaCl10.0g, agar 20.0g, tap water 1.0L, pH7.4, in 103kpa, 121 DEG C of high pressure steam sterilization 30min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108192888A (en) * | 2018-01-29 | 2018-06-22 | 大连大学 | A kind of method of marine low temperature gamma-lactam enzyme production (-) gamma-lactam |
| CN108410749A (en) * | 2018-01-29 | 2018-08-17 | 大连大学 | The method that marine low temperature (+) gamma-lactam enzyme asymmetric hydrolysis prepares (-) gamma-lactam |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1348765A1 (en) * | 2002-03-28 | 2003-10-01 | Council of Scientific and Industrial Research | Process for the preparation of optically active azabicyclo heptanone derivatives |
| CN1807610A (en) * | 2006-01-23 | 2006-07-26 | 迟乃玉 | Method for producing low temperature cellulase using microbe fermentation |
| CN102719378A (en) * | 2012-06-13 | 2012-10-10 | 江南大学 | Method for preparing (-) gamma-lactam by catalysis asymmetry of microorganism |
-
2015
- 2015-02-16 CN CN201510084710.1A patent/CN104630196A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1348765A1 (en) * | 2002-03-28 | 2003-10-01 | Council of Scientific and Industrial Research | Process for the preparation of optically active azabicyclo heptanone derivatives |
| CN1807610A (en) * | 2006-01-23 | 2006-07-26 | 迟乃玉 | Method for producing low temperature cellulase using microbe fermentation |
| CN102719378A (en) * | 2012-06-13 | 2012-10-10 | 江南大学 | Method for preparing (-) gamma-lactam by catalysis asymmetry of microorganism |
Non-Patent Citations (4)
| Title |
|---|
| DAWEI SONG等: "Homology modeling and docking studies of BjGL,a novel(+)gamma-lactamasefrom Bradyrhizobium japonicum", 《JOURNAL OF MOLECULAR GRAPHICS AND MODELLING》 * |
| 王佳宜: "一种(+)γ-内酰胺酶的表达纯化与没血性质的初步研究", 《北京化工大学硕士研究生学位论文》 * |
| 王建军等: "微生物来源的γ-内酰胺酶研究进展", 《微生物学报》 * |
| 黄亚东等: "《啤酒生产技术》", 28 February 2013, 中国轻工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108192888A (en) * | 2018-01-29 | 2018-06-22 | 大连大学 | A kind of method of marine low temperature gamma-lactam enzyme production (-) gamma-lactam |
| CN108410749A (en) * | 2018-01-29 | 2018-08-17 | 大连大学 | The method that marine low temperature (+) gamma-lactam enzyme asymmetric hydrolysis prepares (-) gamma-lactam |
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