CN104615072A - Method for collecting ultrathin section - Google Patents
Method for collecting ultrathin section Download PDFInfo
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- CN104615072A CN104615072A CN201510009128.9A CN201510009128A CN104615072A CN 104615072 A CN104615072 A CN 104615072A CN 201510009128 A CN201510009128 A CN 201510009128A CN 104615072 A CN104615072 A CN 104615072A
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
- G05B19/00—Programme-control systems
- G05B19/02—Programme-control systems electric
- G05B19/04—Programme control other than numerical control, i.e. in sequence controllers or logic controllers
- G05B19/05—Programmable logic controllers, e.g. simulating logic interconnections of signals according to ladder diagrams or function charts
Abstract
The invention discloses a method for collecting an ultrathin section. The method for collecting the ultrathin section includes the steps: arranging running speed of a driving wheel motor of the ultrathin section and moisturizing delay time of an automatic moisturizing module of a diamond cutter water tank through a wireless handhold controller; control an ultrathin section collector to collect the ultrathin section through a PLC (programmable logic controller); during the process of collecting the ultrathin section, control the automatic moisturizing module of the diamond cutter water tank to moisturize the diamond cutter water tank through the PLC; during the process of collecting the ultrathin section, collect the swaying frequency of a slicer blade arm through a swaying counting collecting module of the slicer blade, and transforming to be a level signal which can be identified by the PLC; through the PLC, receive the level signal and calculate pulse number, so as to account the slicer blade swaying frequency; meanwhile, return the swaying frequency to the wireless handhold controller by the PLC. The collecting method of the ultrathin section provided by the invention is simple in mechanical structure, and convenient to operate by users and maintain system, and able to stably collect the ultrathin section for a long time.
Description
Technical field
The present invention relates to experimental provision field, particularly a kind of collection method of ultra-thin section.
Background technology
In order to understand brain function structure, researchist must draw the basic neural network of brain, loop due to aixs cylinder wherein and dendron is extremely accurate complicated, is therefore the effective ways building neural circuit by Electronic Speculum to ultra-thin section imaging and then to neural circuit three-dimensional reconstruction.But because required ultra-thin section substantial amounts causes manpower greatly to consume, manually fish for section link and become no longer feasible.There is Railway Project in the automatic collection technique of current ultra-thin section: one, mechanical transmission structure is too complicated, and make running part control strategy become complicated, people is for adding system complexity.The operation of two, loading and unloading drum and installation travelling belt is very loaded down with trivial details.Three, collector duct position adjustment of cutting into slices lacks dirigibility.Four, cannot controlled in wireless be realized, user is bound by operator's console.Five, dynamic moisturizing can not be realized.
Summary of the invention
Technical matters to be solved by this invention is to provide a kind of collection method of ultra-thin section.
For solving the problems of the technologies described above, the invention provides a kind of collection method of ultra-thin section, comprising
The driving wheel motor travelling speed of ultra-thin section gatherer is set by wireless handheld controller, the moisturizing delay time of diamond cutter tank automatic water supplement module;
The collection that ultra-thin section gatherer carries out ultra-thin section is controlled by PLC;
In the collection process of ultra-thin section, control diamond cutter tank automatic water supplement module by PLC and moisturizing is carried out to diamond cutter tank;
In the collection process of ultra-thin section, by slicer disc arm swing counting acquisition module, slicer disc arm swing number of times is gathered, and be converted into the level signal that PLC can identify;
Receive described level signal by PLC and calculate umber of pulse, for adding up microtome tool arm swing arm number of times, swing arm number of times is returned to described wireless handheld controller by PLC simultaneously.
Further, described in the collection process of ultra-thin section, by PLC control diamond cutter tank automatic water supplement module, moisturizing is carried out to diamond cutter tank and comprise:
Described diamond cutter tank automatic water supplement module comprises diamond cutter tank, probe-type level sensor, micro-injection pump and the second motor driver;
By probe-type level sensor, described diamond cutter tank water level is responded to;
When described diamond cutter tank water level is lower than water level probe height, described probe-type level sensor produces the first induced signal, after described PLC receives described first induced signal, after the moisturizing delay time of described setting, described PLC controls described second motor driver and drives described micro-injection pump to move, improve diamond cutter tank water level, when the water surface touches water level probe, probe-type level sensor produces the second induced signal, and described PLC receives the second motor driver described in described second actuated signal control to be stopped driving the motion of described micro-injection pump.
Further, describedly collection carried out to slicer disc arm swing number of times comprise by slicer disc arm swing counting acquisition module:
Described slicer disc arm swing counting acquisition module comprises microtome tool arm and photoelectric coupling circuit;
By photoelectric coupling circuit, the swing arm stroke of described microtome tool arm is converted into square-wave signal, and square-wave signal is converted into the level signal that described PLC can identify;
Described level signal after described conversion is sent to described PLC.
Further, described ultra-thin section gatherer comprises base, feed wheel motor, supporting construction, for adjusting the drive wheel mechanism of pressure between driving wheel and travelling belt, ribbon supply Bobbin structure, take-up Bobbin structure, for fixing trough of belt pinch roller, the position of pinch roller and guide rail front end roller bearing three also adjusts the position of three and tank and the guide rail of immersion angle degree and position adjustment structure, take-up pulley friction structure, feed wheel friction structure, the Timing Belt structure of described drive wheel mechanism and described take-up pulley friction structure synchronous axial system is made by Timing Belt engaged transmission, for making the travelling belt tensioning between ribbon supply drum to guide rail front end roller bearing, make the tension structure of travelling belt stable drive and the first angle sheave for leading to driving-belt, described drive wheel mechanism, ribbon supply Bobbin structure, take-up Bobbin structure, tension structure, described first angle sheave, described guide rail and position adjustment structure are separately fixed in described supporting construction, described supporting construction, feed wheel motor are separately fixed on described base, described feed wheel friction structure drives described ribbon supply Bobbin structure to rotate by frictional disk, and when travelling belt is subject to certain tensioning, described feed wheel friction structure and ribbon supply Bobbin structure skid, described take-up pulley friction structure drives described take-up Bobbin structure to rotate by frictional disk, and when travelling belt is subject to certain tensioning, described take-up pulley friction structure and take-up Bobbin structure skid, described feed wheel motor is connected by shaft coupling with feed wheel friction structure, described feed wheel friction structure is used for when described feed wheel motor is static providing resistance for feed wheel drum, when making described drive wheel mechanism drag travelling belt, described ribbon supply Bobbin structure can not discharge travelling belt because of inertia effect and cause tension force to reduce, be placed on described take-up Bobbin structure position by packing greatly drum during described feed wheel motor movement, ceases to be busy dish is placed on described ribbon supply Bobbin structure position, can realize inner wrapping drum packing function, travelling belt, successively by described ribbon supply Bobbin structure, described tension structure, described first angle sheave, described guide rail and position adjustment structure, described drive wheel mechanism, finally arrives described take-up drum, thus realizes the collection being loaded with ultra-thin section travelling belt.
Further, described tension structure comprises support, two slide bars, two linear bearings, back shaft, slide block, stretching pulley and two bearings; Described back shaft one end is connected with described stretching pulley by bearing, the described back shaft other end is connected with described slide block, described slide block is connected with described linear bearing external diameter and passes through screw-clamped, the internal diameter of described linear bearing connects described slide bar one end makes described slide block by described linear bearing and described slide bar generation relative motion, two described slide bars are socketed respectively the 4th spring, the described slide bar other end is connected with described support, and described stretching pulley applies pressure by described 4th spring to the travelling belt in tensioning race and makes travelling belt tensioning.
Further, described take-up pulley friction structure and feed wheel friction structure comprise belt wheel flange, two bearings, Timing Belt wheel shaft, synchronous pulley axle sleeve, the second spring, frictional disk, belt shafts respectively; Described belt wheel flange two ends connect a described bearing respectively; Described bearing is for loading described Timing Belt wheel shaft and described belt shaft; Described Timing Belt wheel shaft is hollow structure and axle inner opposite end is provided with step, and described Timing Belt wheel shaft runs through described bearing and connects described frictional disk; Axle one end of described frictional disk is provided with step, and described frictional disk can be slid axially relative to described Timing Belt wheel shaft within the specific limits while rotating together with Timing Belt wheel shaft; Described synchronous pulley axle sleeve to be socketed on described Timing Belt wheel shaft and to lock; Described second spring one end is socketed in described synchronous pulley axle sleeve, and the other end is socketed on described frictional disk, thus provides pressure for described frictional disk and described belt shaft; Described belt shaft runs through described bearing and connects described frictional disk, and the circular shaft shoulder of its one end is used for and described frictional disk friction gearing.
Further, described drive wheel mechanism comprises angular adjustment frame, driving wheel baffle plate, electric machine support, four bearings, driving wheel major axis, driving wheel minor axis, two driving wheels, four support columns, driving wheel motor, motor shaft sleeves, the 3rd spring and hexagonal spline; Described angular adjustment frame one end is circular hole, and the described belt wheel flange in described circular hole and described take-up pulley friction structure is socketed; The described angular adjustment frame other end is ring groove, and described ring groove is connected with described driving wheel baffle plate, for adjusting angle between driving wheel on described driving wheel baffle plate and described angular adjustment frame; Described driving wheel baffle plate one side connects described angular adjustment frame, and another side is equipped with two bearings, for connecting described driving wheel major axis and described driving wheel minor axis one end; Two bearings are equipped with in described electric machine support side, for connecting described driving wheel major axis and the described driving wheel minor axis other end; Described driving wheel major axis one end runs through described electric machine support, described driving wheel, described driving wheel baffle plate, described angular adjustment frame connection synchronous pulley successively, and the described driving wheel major axis other end is spline; Described driving wheel minor axis runs through successively and connects described electric machine support, described driving wheel and described driving wheel baffle plate; Two described driving wheels are socketed on described driving wheel major axis and described driving wheel minor axis respectively; Described driving wheel baffle plate runs through by screw one end that four described support columns connect described electric machine support; Described driving wheel motor connects the described electric machine support other end, described driving wheel motor axle is through described driving wheel motor support other end center hole, described motor shaft sleeve is socketed on motor shaft and outside is provided with threaded hole, and described motor shaft sleeve and motor shaft realize position by jackscrew and lock; The described motor shaft sleeve other end is socketed described hexagonal spline, and described motor shaft sleeve inside is provided with described 3rd spring; Described 3rd spring applies pressure to described hexagonal spline, and described hexagonal spline is connected with described spline; Described driving wheel motor connects described first motor driver.
Further, described hexagonal spline sidewall is provided with spacer pin, described spacer pin is slidably installed in described motor shaft sleeve sidewall circular hole, is of coupled connections for described motor and described driving wheel long shaft transmission and removing when loading travelling belt between described motor and described driving wheel.
Further, described ribbon supply Bobbin structure and take-up Bobbin structure comprise drum, pressure zone clamp, snak link and the first spring respectively; Described wire coil main body side sidewall is provided with the perforate of being convenient to install described travelling belt and pressure zone clamp; Described wire coil main body is also provided with pipe near the homonymy of described perforate; The inwall side of described pipe is provided with step, and described step is provided with the first register pin, and described first spring one end is socketed on described first register pin and makes described first spring location; Described round tube inner wall opposite side and drum center pit tangent, its circular side wall is provided with a through hole; Described snak link is slidably installed in described through hole, and described snak link sidewall is provided with the sidewall of spacer pin for preventing described snak link from departing from described pipe; One end of described snak link is provided with the second register pin, the first spring other end described in described second register pin noose.
Further, described guide rail and position adjustment structure comprise guide rail structure and position adjustment structure, described guide rail structure is connected with described position adjusting mechanism, and described position adjusting mechanism is for adjusting guide rail structure and diamond cutter sink area and guide rail front end roller bearing immersion angle degree.
Further, described position adjustment structure comprises X-direction adjust structure, Y-direction adjust structure and angle-regulation structure; Described X-direction adjust structure and described Y-direction adjust structure adopt dovetail groove and screw structure, and described angle-regulation structure adopts hinge structure; The dovetail groove slide block of described X-direction adjust structure is fixedly connected in described supporting construction, dovetail groove slide rail one end of described X-direction adjust structure is welded on dovetail groove slide rail one end of described Y-direction adjust structure, the dovetail groove slide block of described Y-direction adjust structure connects described angle-regulation structure one leaf of hinge, and described guide rail structure is fixedly connected with another leaf of hinge of described angle-regulation structure.
Further, described guide rail structure comprises the first guide rail baffle, the second guide rail baffle, the 3rd guide rail baffle, the first support column, the second support column, described trough of belt pinch roller, two described pinch rollers and the guide rail front end roller bearing for leading for travelling belt; Described second guide rail baffle and the 3rd guide rail baffle are separately fixed on described angle-regulation structure one leaf of hinge; A described pinch roller is socketed in the second guide rail baffle and the 3rd guide rail baffle sidewall, and another pinch roller described is socketed in described first guide rail baffle and described 3rd guide rail plate washer sidewall; Wherein, support column described in first is fixed in the middle of described first guide rail baffle and the 3rd guide rail baffle sidewall, for robust structure; Described trough of belt pinch roller, described pinch roller and described guide rail front end roller bearing are socketed between described first guide rail baffle and the 3rd guide rail baffle respectively; Support column described in second is fixed in the middle of described second guide rail baffle and the 3rd guide rail baffle sidewall, for robust structure; Described second guide rail baffle and the first guide rail baffle are combined to form a crack, are conveniently loaded between described pinch roller and described guide rail front end roller bearing by travelling belt described in crack.
Further, describedly control by PLC the collection that ultra-thin section gatherer carries out ultra-thin section and comprise:
The order for controlling described driving wheel motor travelling speed from described wireless handheld controller is received by described PLC;
The first motor driver controlling to be connected with described driving wheel motor by high-speed pulse output mouth after described PLC receives order drives described driving wheel motor to operate, described driving wheel motor by described motor shaft sleeve, described hexagonal spline and described driving wheel long shaft transmission, thus makes the described driving wheel on described driving wheel major axis and described synchronous pulley rotate;
Described driving wheel drives belt motion as driving wheel by friction force, synchronous pulley simultaneously on described driving wheel major axis drives the synchronous pulley on described take-up pulley friction structure to realize take-up pulley friction structure by Timing Belt and take-up Bobbin structure rotates, thus travelling belt is involved from ribbon supply Bobbin structure, and by described stretching pulley, described first angle sheave, described pinch roller in described guide rail and position adjustment structure, described guide rail front end roller bearing, described trough of belt pinch roller, driving takes turns to take-up drum, thus the motion process completed from ribbon supply drum to take-up drum.
The collection method of ultra-thin section provided by the invention, can dynamically moisturizing, and physical construction is simple, simple and convenient user operation, and system is convenient to safeguard, and long-time stable can collect ultra-thin section.
Accompanying drawing explanation
The structured flowchart of the ultra-thin section auto-collection system based on controlled in wireless that Fig. 1 provides for the embodiment of the present invention;
The Facad structure schematic diagram of the ultra-thin section gatherer that Fig. 2 provides for the embodiment of the present invention;
The inverse layer structure schematic diagram of the ultra-thin section gatherer that Fig. 3 provides for the embodiment of the present invention;
The structural representation of the ribbon supply Bobbin structure that Fig. 4 provides for the embodiment of the present invention or take-up Bobbin structure;
The cross-sectional view of the belt wheel friction structure that Fig. 5 provides for the embodiment of the present invention or feed wheel friction structure;
The structural representation of the drive wheel mechanism that Fig. 6 provides for the embodiment of the present invention;
The structural representation of the guide rail that Fig. 7 provides for the embodiment of the present invention and position adjustment structure;
The tension structure schematic diagram that Fig. 8 provides for the embodiment of the present invention;
The schematic diagram that the travelling belt that Fig. 9 provides for the embodiment of the present invention transmits.
Embodiment
The collection method of the ultra-thin section that the embodiment of the present invention provides is implemented by the ultra-thin section auto-collection system based on controlled in wireless.See Fig. 1, the ultra-thin section auto-collection system based on controlled in wireless comprises ultra-thin section gatherer, the first motor driver, diamond cutter tank automatic water supplement module, slicer disc arm swing counting acquisition module, wireless handheld controller, the second wireless data transmission module and PLC.First motor driver connects the driving wheel motor 20 (see Fig. 6) of ultra-thin section gatherer.
Wherein, diamond cutter tank automatic water supplement module is used for for diamond cutter tank automatic water supplement.Diamond cutter tank automatic water supplement module can comprise diamond cutter tank, probe-type level sensor, micro-injection pump and the second motor driver.Second motor driver connects micro-injection pump.Micro-injection pump syringe needle stretches to diamond cutter tank side.Probe-type level sensor middle probe stretches to diamond cutter tank opposite side.Diamond cutter tank can adopt the diamond cutter tank of LEICA company.
Slicer disc arm swing counting acquisition module comprises microtome tool arm and photoelectric coupling circuit.Photoelectric coupling circuit is exactly that the swing arm stroke of microtome tool arm is converted into square-wave signal, and square-wave signal is converted into the level signal that PLC can identify.Microtome tool arm can adopt LEICA microtome tool arm.
Wireless handheld controller is for arranging driving wheel motor 20 travelling speed of ultra-thin section gatherer.The moisturizing delay time of diamond cutter tank automatic water supplement module, shows tool arm simultaneously and adds up number of oscillations, and carries out exchanges data by the second wireless data transmission module and PLC.Wherein, wireless handheld controller contains touch-screen, touch-screen is used for display and arranges systematic parameter, the parameter arranged by touch-screen is transferred to the first wireless data transmission module by serial communication mode, and then is sent to PLC (data that namely the second wireless data transmission module transmits send PLC to by serial communication mode) by the first wireless data transmission module by the second wireless data transmission module.Be sent to the first wireless data transmission module by the parameter (the microtome tool arm swing arm number of times that PLC adds up) of system feedback by the second wireless data transmission module, and then transmit back touch-screen by the first wireless data transmission module by serial communication.
PLC connects the first motor driver, the second motor driver respectively by high-speed pulse output mouth.PLC is by high-speed pulse input port linking probe formula level sensor and photoelectric coupling circuit respectively.The one high-speed pulse input port, road of PLC receives the level signal that produced by photoelectric coupled circuit and calculates umber of pulse, and for adding up microtome tool arm swing arm number of times, swing arm number of times is returned to wireless handheld controller by wireless data transmission module by PLC simultaneously.And add up microtome tool arm swing arm number of times be in order to add up section collect number.
Another high-speed pulse input port, road of PLC receives the induced signal produced by probe-type level sensor, when diamond cutter tank water level is lower than water level probe height, probe-type level sensor produces the first induced signal, after PLC receives the first induced signal (high level), after the moisturizing delay time of setting, control the second motor driver and drive micro-injection pump motion, improve diamond cutter tank water level, when the water surface touches water level probe, probe-type level sensor produces the second induced signal (low level), PLC receives the second actuated signal control second motor driver to be stopped driving micro-injection pump motion.Certainly in another embodiment, the first induced signal can be low level, and the second induced signal can be high level.
Another road high-speed pulse output mouth of PLC controls the first motor driver and drives ultra-thin section gatherer by the speed running arranged receiving motor speed parameters that the second wireless data transmission module imports into.
Shown in Fig. 2-9, a kind of ultra-thin section gatherer that the embodiment of the present invention provides, comprise base 1c, feed wheel motor 8, supporting construction 2, for adjusting the drive wheel mechanism 4 of pressure between driving wheel and travelling belt, for providing the ribbon supply Bobbin structure 1b of the travelling belt of sky, for collecting the take-up Bobbin structure 1a of travelling belt, for fixing trough of belt pinch roller 33, the position of pinch roller 34 and guide rail front end roller bearing 25 three also adjusts the position of three and tank and the guide rail of immersion angle degree and position adjustment structure 5, take-up pulley friction structure 2a, feed wheel friction structure 2b, the synchronous pulley 6 of drive wheel mechanism 4 and take-up pulley friction structure 2a synchronous axial system is made by Timing Belt engaged transmission, for making the travelling belt tensioning between ribbon supply drum to guide rail front end roller bearing 25, make the tension structure 7 of travelling belt stable drive and the first angle sheave 56 for leading to travelling belt, drive wheel mechanism 4, ribbon supply Bobbin structure 1b, take-up Bobbin structure 1a, tension structure 7, first angle sheave 56 and guide rail and position adjustment structure 5 are separately fixed in supporting construction 2, supporting construction 2, feed wheel motor 8 are separately fixed on base 1c, wherein, feed wheel friction structure 2b drives described ribbon supply Bobbin structure 1b to rotate by frictional disk, and when travelling belt is subject to certain tensioning, described feed wheel friction structure 2b and ribbon supply Bobbin structure 1b skids, take-up pulley friction structure 2a drives take-up Bobbin structure to rotate by frictional disk, and when travelling belt is subject to certain tensioning, take-up pulley friction structure 2a and take-up Bobbin structure skid, feed wheel motor 8 is connected by shaft coupling with feed wheel friction structure 2b, feed wheel friction structure 2b is used for when feed wheel motor 8 is static providing resistance for feed wheel drum, when making drive wheel mechanism 4 drag travelling belt, ribbon supply Bobbin structure 1b can not discharge travelling belt because of inertia effect and cause tension force to reduce, be placed on take-up Bobbin structure 1a position by packing greatly drum when feed wheel motor 8 moves, ceases to be busy dish is placed on ribbon supply Bobbin structure 1b position, can realize inner wrapping drum packing function.Travelling belt, successively by ribbon supply Bobbin structure 1b, tension structure 7, first angle sheave 56, guide rail and position adjustment structure 5, drive wheel mechanism 4, finally arrives take-up Bobbin structure 1a, thus realizes the collection being loaded with ultra-thin section travelling belt.
See Fig. 4, ribbon supply Bobbin structure 1b and take-up Bobbin structure 1a comprises drum 9, pressure zone clamp 10, snak link 13 and the first spring 11 respectively.Drum 9 main body side sidewall is provided with the perforate of being convenient to install travelling belt and pressure zone clamp 10.Drum 9 main body is also provided with pipe 12 near the homonymy of perforate.The inwall side of pipe 12 is provided with step.Step is provided with the first register pin (not shown).First spring 11 one end is socketed on the first register pin and the first spring 11 is located.Pipe 12 inwall opposite side is tangent with drum 9 center pit, and its circular side wall is provided with a through hole.Snak link 13 is slidably installed in through hole.Snak link 13 is a rectangle structure, and middle elongated hole side is circular, is provided with a register pin outside round nose elongated hole.Snak link 13 sidewall is provided with the sidewall of spacer pin for preventing snak link 13 from departing from pipe 12, the register pin socket spring other end outside round nose elongated hole.One end of snak link 13 is provided with the second register pin, second register pin noose first spring 11 other end.During dismounting drum, user can press the circular tube structure of snak link 13 and drum, and pipe 12 and snak link 13 against the force of the spring are subjected to displacement, and the circular side of snak link 13 departs from take-up wheel shaft or ribbon supply wheel shaft.When drum is installed, user can press the circular tube structure of snak link and drum, the circular side of snak link is made to press close to take-up wheel shaft or ribbon supply wheel shaft, when user is not according to pressing spring button 13, first spring 11 contacts making snak link 13 extrude with the ribbon supply wheel shaft in feed wheel friction structure, realize ribbon supply wheel shaft and for transmission between drum, or the first spring 11 contacts making snak link 13 extrude with the take-up wheel shaft in take-up pulley friction structure, realizes transmission between take-up wheel shaft and take-up reel.
See Fig. 5, take-up pulley friction structure 2a or feed wheel friction structure 2b comprises belt wheel flange 41 respectively (namely when for being take-up pulley flange during take-up pulley friction structure 2a, when for being feed wheel flange during feed wheel friction structure 2b), two bearings 42, Timing Belt wheel shaft 44, synchronous pulley axle sleeve 45, second spring 46, frictional disk 47, belt shaft 43 (when for being take-up wheel shaft during take-up pulley friction structure 2a, when for be ribbon supply wheel shaft during feed wheel friction structure 2b); Belt wheel flange 41 two ends connect a bearing 42 respectively; Bearing 42 is for loading Timing Belt wheel shaft 44 and belt shaft 43; Timing Belt wheel shaft 44 is for hollow structure and axle inner opposite end is provided with step, and Timing Belt wheel shaft 44 runs through bearing 42 and connects frictional disk 47; Axle one end of frictional disk 47 is provided with step, and frictional disk 47 can be slid axially relative to Timing Belt wheel shaft 44 within the specific limits while rotating together with Timing Belt wheel shaft 44; Synchronous pulley axle sleeve 45 to be socketed on Timing Belt wheel shaft 44 and to pass through screw locking; Second spring 46 one end is socketed in synchronous pulley axle sleeve 45, and the other end is socketed on frictional disk 47, thus provides pressure for frictional disk 47 and belt shaft 43; Belt shaft 43 runs through bearing 42 and connects frictional disk 47, and the circular shaft shoulder of its one end is used for and frictional disk 47 friction gearing.Wherein, the Timing Belt wheel shaft 44 of take-up pulley friction structure 2a connects synchronous pulley 6 (see Fig. 2, the synchronous pulley that namely diameter is larger).
See Fig. 6, drive wheel mechanism 4 comprises angular adjustment frame 14, driving wheel baffle plate 21, electric machine support 17, four bearings, driving wheel major axis 15, driving wheel minor axis 23, two driving wheels 22, four support columns 16, driving wheel motor 20, motor shaft sleeve 24, the 3rd spring 19 and hexagonal spline 18; Angular adjustment frame 14 one end is circular hole, and the belt wheel flange in circular hole and take-up pulley friction structure 2a is socketed; Angular adjustment frame 14 other end is ring groove, and ring groove is connected with driving wheel baffle plate 21, for adjusting angle between driving wheel 22 on driving wheel baffle plate 21 and angular adjustment frame 14; Driving wheel baffle plate 21 one side is by screw connection angle adjustment rack 14, and another side is equipped with two bearings, for connecting driving wheel major axis 15 and driving wheel minor axis 23 one end; Two bearings are equipped with in electric machine support 17 side, for connecting driving wheel major axis 15 and driving wheel minor axis 23 other end; Driving wheel major axis 15 one end runs through electric machine support 17 successively, driving wheel 22, driving wheel baffle plate 21, angular adjustment frame 14 connect synchronous pulley 6 (see Fig. 2, the synchronous pulley that namely diameter is less), and driving wheel major axis 15 other end is spline; Driving wheel minor axis 23 runs through successively and connects electric machine support 17, driving wheel and driving wheel baffle plate 21; Two driving wheels 22 are socketed on driving wheel major axis 15 and driving wheel minor axis 23 respectively; Driving wheel baffle plate 21 runs through by screw one end that four support columns 16 connect electric machine support 17; Driving wheel motor 20 connects electric machine support 17 other end.Driving wheel motor 20 also connects the first motor driver.Motor shaft is through electric machine support 17 other end center hole.Motor shaft sleeve 24 is socketed on motor shaft and outside is provided with threaded hole, and motor shaft sleeve 24 and motor shaft realize position by jackscrew and lock; Motor shaft sleeve 24 other end socket hexagonal spline 18, motor shaft sleeve 24 inside is provided with the 3rd spring 19; 3rd spring 19 applies pressure to hexagonal spline 18, and hexagonal spline 18 is connected with spline.Hexagonal spline 18 sidewall is provided with spacer pin, and spacer pin is slidably installed in motor shaft sleeve 24 sidewall circular hole, is of coupled connections between motor and driving wheel for motor and driving wheel major axis 15 transmission and removing when loading travelling belt.Drive wheel mechanism 4 is socketed in take-up pulley flange side in take-up pulley friction structure, to be fixed on take-up pulley friction structure by take-up pulley flange screw thread and nut and to form certain angle with take-up pulley friction structure, for adjusting the angle of driving wheel and guide rail structure and take-up Bobbin structure, pressure between adjustment driving wheel and travelling belt.Drive wheel mechanism is socketed in feed wheel flange side in feed wheel friction structure, to be fixed on feed wheel friction structure by feed wheel flange screw thread and nut and to form certain angle with feed wheel friction structure, for adjusting the angle of driving wheel and guide rail structure and ribbon supply Bobbin structure 1b, pressure between adjustment driving wheel and travelling belt.
See Fig. 7, guide rail and position adjustment structure 5 comprise guide rail structure 28 and position adjustment structure 38, guide rail structure is connected with position adjusting mechanism 38 with 28, and position adjusting mechanism is for adjusting guide rail structure and diamond cutter sink area and guide rail front end roller bearing 25 immersion angle degree.Position adjustment structure 38 comprises X-direction adjust structure 39, Y-direction adjust structure 36 and angle-regulation structure 37; X-direction adjust structure 39 and Y-direction adjust structure 36 adopt dovetail groove and screw structure, and angle-regulation structure 37 adopts hinge structure; The dovetail groove slide block 40 of X-direction adjust structure 39 is fixedly connected with on the support structure 2, dovetail groove slide rail 30 one end of X-direction adjust structure 39 is welded on dovetail groove slide rail 42 one end of Y-direction adjust structure 36, dovetail groove slide block 41 connection angle adjust structure 37 1 leaf of hinge 35 of Y-direction adjust structure 36.
Guide rail structure 28 is fixedly connected with another leaf of hinge 29 of angle-regulation structure 37.Guide rail structure comprises the first guide rail baffle 26, second guide rail baffle 27, the 3rd guide rail baffle 31, first support column 32, second support column 32 ', trough of belt pinch roller 33, two pinch rollers 34 and the guide rail front end roller bearing 25 for leading for travelling belt.Second guide rail baffle 27 and the 3rd guide rail baffle 31 are separately fixed on angle-regulation structure 37 1 leaf of hinge.One pinch roller 34 is socketed in the second guide rail baffle 27 and the 3rd guide rail baffle 31 sidewall, and another pinch roller 34 is socketed in the first guide rail baffle 26 and the 3rd guide rail baffle 31 sidewall.First support column 32 is screwed in the middle of the first guide rail baffle 26 and the 3rd guide rail baffle 31 sidewall, for robust structure; Trough of belt pinch roller 33, pinch roller 34 and guide rail front end roller bearing 25 are socketed between the first guide rail baffle 26 and the 3rd guide rail baffle 31 respectively; Second support column 32 ' is fixed in the middle of the second guide rail baffle 27 and the 3rd guide rail baffle 31 sidewall, for robust structure; Second guide rail baffle 27 and the first guide rail baffle 26 are combined to form a crack, are conveniently loaded between pinch roller 34 and guide rail front end roller bearing 25 by crack travelling belt, thus effectively avoid load the loaded down with trivial details of travelling belt by threading mode in travelling belt loop.
See Fig. 8, tension structure 7 comprises support 64, two slide bars 63, two linear bearings 62, back shaft 59, slide block 61, stretching pulley 57 and two bearings 60; Back shaft 59 one end is connected with stretching pulley 57 by bearing 60, back shaft 59 other end is connected with slide block 61, slide block 61 is connected with linear bearing 62 external diameter and passes through screw-clamped, the internal diameter of linear bearing 62 connects back shaft 59 one end makes slide block 61, by linear bearing 62 and back shaft 59, relative motion occur, two back shafts 59 are socketed respectively the 4th spring (not shown), back shaft 59 other end is connected with support 64, and stretching pulley 57 applies pressure by the 4th spring to the travelling belt in stretching pulley 57 groove and makes travelling belt tensioning.
See Fig. 9, travelling belt 51 successively by ribbon supply drum 58, stretching pulley 57, first angle sheave 56, pinch roller 34, guide rail front end roller bearing 25, trough of belt pinch roller 33, final arrive take-up drum 49.Ribbon supply drum 58 is for providing empty travelling belt, and stretching pulley 57 is for making ribbon supply drum 58, to the travelling belt tensioning between guide rail front end roller bearing 25, making stable drive.First angle sheave 56 is for guiding.First angle sheave 56 is by a back shaft, and two bearings and an angle sheave form.Back shaft one end is connected with supporting construction, and the back shaft other end is socketed two bearings and angle sheave respectively.Pinch roller 34, for making travelling belt not rise and fall in the horizontal direction, not derailing, makes travelling belt run stably.Guide rail front end roller bearing 25 and trough of belt pinch roller 33 combine and make travelling belt and the water surface form less angle, are easy to collect ultra-thin section from tank 53.Race both sides and the pinch roller 34 of trough of belt pinch roller 33 contact travelling belt both sides respectively and are used for preventing travelling belt from rising and falling in the horizontal direction, derailing, thus conveying belt is run stably, the middle race of trough of belt pinch roller 33 makes ultra-thin section pass through and avoids being subject to the extruding of race both sides.Driving wheel 22 drives conveyer belt as driving wheel by friction mode, and the race in the middle of driving wheel 22 makes ultra-thin section pass through and avoids being subject to the extruding of race both sides.Take-up drum is loaded with the travelling belt of ultra-thin section for collecting.
The course of work of the collection method of the ultra-thin section that the embodiment of the present invention provides is as follows: first wireless handheld controller comprises three parameters: motor rotation speed, automatic water supplement time and tool arm number of oscillations, wherein, motor rotation speed and automatic water supplement time arrange to outgoing data command by touch-screen, cutter swing arm number of times is feedback parameter, on the touchscreen just display.
First via signal: motor rotation speed order is sent to the second wireless data transmission module by the first wireless data transmission module of wireless handheld controller, PLC is given again by the second wireless data transmission module, PLC controls the first motor driver by high-speed pulse output mouth after receiving order, first motor driver drives the driving wheel motor 20 in the driving wheel structure 4 of ultra-thin section gatherer, and driving wheel motor 20 makes the driving wheel 22 on driving wheel major axis 15 and synchronous pulley 6 rotate by motor shaft sleeve 24, hexagonal spline 18 and driving wheel major axis 15 transmission.Driving wheel 22 drives belt motion as driving wheel by friction force, and the synchronous pulley 6 simultaneously on driving wheel major axis 15 drives the synchronous pulley 6 on take-up pulley friction structure to realize take-up pulley friction structure by Timing Belt and take-up Bobbin structure rotates.Therefore travelling belt is involved by stretching pulley 57 and the first angle sheave 56 from ribbon supply Bobbin structure 1b, pinch roller 34, guide rail front end roller bearing 25 in guide rail and position adjustment structure, trough of belt pinch roller 33, driving wheel 22, take-up drum complete the motion process from ribbon supply drum to take-up drum.
Second road signal: the moisturizing delay time of setting is sent to the second wireless data transmission module by the first wireless data transmission module of wireless handheld controller, and then sends to PLC by the second wireless data transmission module.When the probe of liquid level lower than probe-type level sensor, first induced signal is sent to PLC by probe-type level sensor, PLC starts timing after receiving signal, when after the moisturizing delay time arriving setting, PLC drives the second electric machine controller, and the second electric machine controller drives micro-injection pump to the moisturizing of diamond cutter tank.When the water surface touches water level probe, probe-type level sensor produces the second induced signal, and PLC receives the second actuated signal control second motor driver and closes, and stops driving micro-injection pump motion.
3rd road signal: slicer disc arm swing cutting photoelectric coupling circuit produces square wave feeding PLC, PLC and signal returned to the touch-screen of wireless handheld controller by wireless data transmission module in order to show tool arm number of oscillations in real time.
The collection method of ultra-thin section provided by the invention is owing to taking above technical scheme, and it has the following advantages:
1, adopt adjustment guide rail position but not the mode of adjustment complete machine position realizes guide rail and diamond cutter sink area regulates, thus make adjustment more flexible.Wherein two-dimensional movement structure adopts small-sized dovetail groove and screw structure, realizes guide rail X-direction and Z-direction two-dimensional movement, and angular adjustment adopts hinge structure, realizes angular adjustment.
2, guide rail baffle side be provided with trough of belt be convenient to install travelling belt, its right-angle structure makes the travelling belt of injection not easily derail.
3, use one group of drive wheel mechanism to drive conveyer belt, need not consider by the driving stationary problem organizing drive wheel mechanism introducing more.
4, driving wheel motor is not when powering on, and cannot rotate, can adopt removable spline joint between driving wheel and driving wheel motor because resistance is large, disconnects linking between motor with driving wheel when upper band by mobile spline, expediently rotating drive wheel during band.
5, the angular adjustment frame of drive wheel mechanism can change angle between guide rail and take-up drum, the friction force between adjustment driving wheel and travelling belt.
6, take-up pulley friction structure or feed wheel friction structure adopt concealed friction structure to realize slipping effect, and user need not regulate friction force.
7, take-up drum and ribbon supply drum adopt snak link structure to facilitate user to dismantle drum.
8, adopt Timing Belt structure between take-up pulley and driving wheel, realize synchronous between take-up pulley with driving wheel and reduce take-up pulley drive motor quantity.
9, can combinationally use with LEICA microtome, there is extensive generalization.
10, in native system, wireless handheld controller freely can realize Electric Machine Control by wireless transmission in the certain limit of laboratory, and user need not be bound by operator's console.
It should be noted last that, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to example to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (10)
1. a collection method for ultra-thin section, is characterized in that, comprises
The driving wheel motor travelling speed of ultra-thin section gatherer is set by wireless handheld controller, the moisturizing delay time of diamond cutter tank automatic water supplement module;
The collection that ultra-thin section gatherer carries out ultra-thin section is controlled by PLC;
In the collection process of ultra-thin section, control diamond cutter tank automatic water supplement module by PLC and moisturizing is carried out to diamond cutter tank;
In the collection process of ultra-thin section, by slicer disc arm swing counting acquisition module, slicer disc arm swing number of times is gathered, and be converted into the level signal that PLC can identify;
Receive described level signal by PLC and calculate umber of pulse, for adding up microtome tool arm swing arm number of times, swing arm number of times is returned to described wireless handheld controller by PLC simultaneously.
2. the collection method of ultra-thin section according to claim 1, is characterized in that, described in the collection process of ultra-thin section, carries out moisturizing comprise by PLC control diamond cutter tank automatic water supplement module to diamond cutter tank:
Described diamond cutter tank automatic water supplement module comprises diamond cutter tank, probe-type level sensor, micro-injection pump and the second motor driver;
By probe-type level sensor, described diamond cutter tank water level is responded to;
When described diamond cutter tank water level is lower than water level probe height, described probe-type level sensor produces the first induced signal, after described PLC receives described first induced signal, after the moisturizing delay time of described setting, described PLC controls described second motor driver and drives described micro-injection pump to move, improve diamond cutter tank water level, when the water surface touches water level probe, probe-type level sensor produces the second induced signal, and described PLC receives the second motor driver described in described second actuated signal control to be stopped driving the motion of described micro-injection pump.
3. the collection method of ultra-thin section according to claim 1, is characterized in that, is describedly carried out collection to slicer disc arm swing number of times comprised by slicer disc arm swing counting acquisition module:
Described slicer disc arm swing counting acquisition module comprises microtome tool arm and photoelectric coupling circuit;
By photoelectric coupling circuit, the swing arm stroke of described microtome tool arm is converted into square-wave signal, and square-wave signal is converted into the level signal that described PLC can identify;
Described level signal after described conversion is sent to described PLC.
4. the collection method of ultra-thin section according to claim 1, it is characterized in that, described ultra-thin section gatherer comprises base, feed wheel motor, supporting construction, for adjusting the drive wheel mechanism of pressure between driving wheel and travelling belt, ribbon supply Bobbin structure, take-up Bobbin structure, for fixing trough of belt pinch roller, the position of pinch roller and guide rail front end roller bearing three also adjusts the position of three and tank and the guide rail of immersion angle degree and position adjustment structure, take-up pulley friction structure, feed wheel friction structure, the Timing Belt structure of described drive wheel mechanism and described take-up pulley friction structure synchronous axial system is made by Timing Belt engaged transmission, for making the travelling belt tensioning between ribbon supply drum to guide rail front end roller bearing, make the tension structure of travelling belt stable drive and the first angle sheave for leading to driving-belt, described drive wheel mechanism, ribbon supply Bobbin structure, take-up Bobbin structure, tension structure, described first angle sheave, described guide rail and position adjustment structure are separately fixed in described supporting construction, described supporting construction, feed wheel motor are separately fixed on described base, described feed wheel friction structure drives described ribbon supply Bobbin structure to rotate by frictional disk, and when travelling belt is subject to certain tensioning, described feed wheel friction structure and ribbon supply Bobbin structure skid, described take-up pulley friction structure drives described take-up Bobbin structure to rotate by frictional disk, and when travelling belt is subject to certain tensioning, described take-up pulley friction structure and take-up Bobbin structure skid, described feed wheel motor is connected by shaft coupling with feed wheel friction structure, described feed wheel friction structure is used for when described feed wheel motor is static providing resistance for feed wheel drum, when making described drive wheel mechanism drag travelling belt, described ribbon supply Bobbin structure can not discharge travelling belt because of inertia effect and cause tension force to reduce, be placed on described take-up Bobbin structure position by packing greatly drum during described feed wheel motor movement, ceases to be busy dish is placed on described ribbon supply Bobbin structure position, can realize inner wrapping drum packing function, travelling belt, successively by described ribbon supply Bobbin structure, described tension structure, described first angle sheave, described guide rail and position adjustment structure, described drive wheel mechanism, finally arrives described take-up drum, thus realizes the collection being loaded with ultra-thin section travelling belt.
5. the collection method of ultra-thin section according to claim 4, is characterized in that, described tension structure comprises support, two slide bars, two linear bearings, back shaft, slide block, stretching pulley and two bearings; Described back shaft one end is connected with described stretching pulley by bearing, the described back shaft other end is connected with described slide block, described slide block is connected with described linear bearing external diameter and passes through screw-clamped, the internal diameter of described linear bearing connects described slide bar one end makes described slide block by described linear bearing and described slide bar generation relative motion, two described slide bars are socketed respectively the 4th spring, the described slide bar other end is connected with described support, and described stretching pulley applies pressure by described 4th spring to the travelling belt in tensioning race and makes travelling belt tensioning.
6. the collection method of ultra-thin section according to claim 5, it is characterized in that, described take-up pulley friction structure and feed wheel friction structure comprise belt wheel flange, two bearings, Timing Belt wheel shaft, synchronous pulley axle sleeve, the second spring, frictional disk, belt shafts respectively; Described belt wheel flange two ends connect a described bearing respectively; Described bearing is for loading described Timing Belt wheel shaft and described belt shaft; Described Timing Belt wheel shaft is hollow structure and axle inner opposite end is provided with step, and described Timing Belt wheel shaft runs through described bearing and connects described frictional disk; Axle one end of described frictional disk is provided with step, and described frictional disk can be slid axially relative to described Timing Belt wheel shaft within the specific limits while rotating together with Timing Belt wheel shaft; Described synchronous pulley axle sleeve to be socketed on described Timing Belt wheel shaft and to lock; Described second spring one end is socketed in described synchronous pulley axle sleeve, and the other end is socketed on described frictional disk, thus provides pressure for described frictional disk and described belt shaft; Described belt shaft runs through described bearing and connects described frictional disk, and the circular shaft shoulder of its one end is used for and described frictional disk friction gearing.
7. the collection method of ultra-thin section according to claim 6, it is characterized in that, described drive wheel mechanism comprises angular adjustment frame, driving wheel baffle plate, electric machine support, four bearings, driving wheel major axis, driving wheel minor axis, two driving wheels, four support columns, driving wheel motor, motor shaft sleeves, the 3rd spring and hexagonal spline; Described angular adjustment frame one end is circular hole, and the described belt wheel flange in described circular hole and described take-up pulley friction structure is socketed; The described angular adjustment frame other end is ring groove, and described ring groove is connected with described driving wheel baffle plate, for adjusting angle between driving wheel on described driving wheel baffle plate and described angular adjustment frame; Described driving wheel baffle plate one side connects described angular adjustment frame, and another side is equipped with two bearings, for connecting described driving wheel major axis and described driving wheel minor axis one end; Two bearings are equipped with in described electric machine support side, for connecting described driving wheel major axis and the described driving wheel minor axis other end; Described driving wheel major axis one end runs through described electric machine support, described driving wheel, described driving wheel baffle plate, described angular adjustment frame connection synchronous pulley successively, and the described driving wheel major axis other end is spline; Described driving wheel minor axis runs through successively and connects described electric machine support, described driving wheel and described driving wheel baffle plate; Two described driving wheels are socketed on described driving wheel major axis and described driving wheel minor axis respectively; Described driving wheel baffle plate runs through by screw one end that four described support columns connect described electric machine support; Described driving wheel motor connects the described electric machine support other end, described driving wheel motor axle is through described driving wheel motor support other end center hole, described motor shaft sleeve is socketed on motor shaft and outside is provided with threaded hole, and described motor shaft sleeve and motor shaft realize position by jackscrew and lock; The described motor shaft sleeve other end is socketed described hexagonal spline, and described motor shaft sleeve inside is provided with described 3rd spring; Described 3rd spring applies pressure to described hexagonal spline, and described hexagonal spline is connected with described spline; Described driving wheel motor connects described first motor driver.
8. the collection method of ultra-thin section according to claim 7, it is characterized in that, described hexagonal spline sidewall is provided with spacer pin, described spacer pin is slidably installed in described motor shaft sleeve sidewall circular hole, is of coupled connections for described motor and described driving wheel long shaft transmission and removing when loading travelling belt between described motor and described driving wheel.
9. the collection method of ultra-thin section according to claim 8, is characterized in that, described ribbon supply Bobbin structure and take-up Bobbin structure comprise drum, pressure zone clamp, snak link and the first spring respectively; Described wire coil main body side sidewall is provided with the perforate of being convenient to install described travelling belt and pressure zone clamp; Described wire coil main body is also provided with pipe near the homonymy of described perforate; The inwall side of described pipe is provided with step, and described step is provided with the first register pin, and described first spring one end is socketed on described first register pin and makes described first spring location; Described round tube inner wall opposite side and drum center pit tangent, its circular side wall is provided with a through hole; Described snak link is slidably installed in described through hole, and described snak link sidewall is provided with the sidewall of spacer pin for preventing described snak link from departing from described pipe; One end of described snak link is provided with the second register pin, the first spring other end described in described second register pin noose.
10. the collection method of ultra-thin section according to claim 9, it is characterized in that, described guide rail and position adjustment structure comprise guide rail structure and position adjustment structure, described guide rail structure is connected with described position adjusting mechanism, and described position adjusting mechanism is for adjusting guide rail structure and diamond cutter sink area and guide rail front end roller bearing immersion angle degree.
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| EP4010680A4 (en) * | 2019-08-06 | 2024-01-24 | Univ Kansas | Apparatus and methods for ultramicrotome specimen preparation |
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| CN104615072B (en) | 2017-06-13 |
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