The discriminating device of a kind of tumour viable tissue and necrotic tissue
Technical field
The present invention relates to the discriminating device of a kind of tumour viable tissue and necrotic tissue.
Background technology
The control of malignant tumour is the focus of medical research, and the tumor tissues as malignant tumour research material has vital role in promotion tumor research progression.The body tumor tissue that underwent operative approach obtains is valuable and limited, to first be used for meeting Clinicopathologic Diagnosis needs, secondly in order to set up tumor tissues sample storehouse, also asepticly can be separated into tumour single cell suspension and carry out the scientific research of tumour or be prepared into the tumor vaccine in autologous tumor source.No matter any application, all requires that the tumor tissues chosen must be tumour viable tissue, should avoid being mixed with necrotic tissue, otherwise be difficult to the demand meeting aforementioned every application.But in actual application, often run into the situation can not distinguishing tumour viable tissue and neoplasm necrosis organizational boundaries very accurately, cause frozen section in art to have to repeat censorship of drawing materials; Or the tumor tissue necrosis's composition in income tumor bank is too much, and scientific research value greatly declines; Or cultivate unsuccessfully because necrotic tissue is too many during preparation tumour single cell suspension, cannot apply further.
Viable tissue and necrotic tissue is distinguished for how simply, fast, pay dogface academician to wait and once adopt integrating sphere light splitting scanning technique and the colour filter optical characteristics of method to dog hind leg high speed bullet wound Hou Shang road Zhou Butong viable tissue of taking pictures to inquire into, find that healthy tissues and devitalized tissue's certain specific wavelength section that is reflected in light exist significant difference, the size of this species diversity and organizational vitality is closely related, organize activity higher, to Transmission light, reflection more obvious.Normal the difference of optical extinction coefficient is organized to occur in 600 ~ 860nm, 450 ~ 600nm and 280 ~ 400nm place with deactivation, wherein comparatively obvious and the part that can be clinical application is the position such as 600 ~ 800nm and 508nm.According to specific spectrum change rule, wavelength is ruddiness and infrared, far infrared part at the light of more than 630nm, and 508nm is blue light components, and the difference of this two portions optical extinction coefficient is that optical method judges normal and the most important theoretical basis of devitalized tissue's boundary.According to this principle, pay dogface academician and wait and adopt camera to be equipped with red, cyan color filter to show the result that wound tissue is taken pictures, this optical differences else really adds optical contrast normally and between devitalized tissue, is beneficial to its line of delimitation of operator's bore hole identification.According to this discovery, pay dogface etc. and succeed in developing surgery colour filter debridement glasses, make surgeon can determine tissue injury scope quickly and accurately, improve debridement quality.
The colour filter debridement glasses paying the developments such as dogface does not have automatic light source, need with the use of the tungsten-iodine lamp of 1300W, and tungsten-iodine lamp can produce high temperature, under it irradiates, is unfavorable for the maintenance of tumor tissue in vitro vigor.The spectrum of tungsten-iodine lamp is continuous spectrum, and except red, blue wave band light, all the other wave band light do not act on for judgement organizational vitality.In addition, colour filter debridement glasses is also convenient not for operator's use of wearing glasses.
Summary of the invention
The present invention seeks to: the discriminating device that a kind of tumour viable tissue and necrotic tissue are provided.
Technical scheme of the present invention is:
A discriminating device for tumour viable tissue and necrotic tissue, comprises bracket base and is positioned at the support top directly over it, and described bracket base is fixedly connected with by the bracket upright post that erects with the edge on support top; Be provided with illuminator below described support top, be irradiated to bracket base, an eyepiece frame is installed at opposite side edge, support top, and eyepiece frame is embedded with spectral filter.
Preferably, described eyepiece frame is flexibly connected with support top.
Preferably, described bracket base is built-in with semiconductor chilling plate.
Preferably, described illuminator adopts red globe lamp, and described spectral filter adopts the dull and stereotyped HB10 red glass of 1.5-2.5mm.
Or described illuminator adopts blue-ray light, and described spectral filter adopts dull and stereotyped blue glass.
Or described illuminator comprises red globe lamp and blue-ray light, spectral filter comprise be suitable for respectively left eye, right eye viewing dull and stereotyped red glass and dull and stereotyped blue glass.
Preferably, the intensity of light of described illuminator can stepless changing.
Preferred further, the light Wavelength tunable of described illuminator.
Advantage of the present invention is:
1. the discriminating device of tumour viable tissue of the present invention and necrotic tissue, adopt light-illuminating tumor tissues, observe with spectral filter, the interference of other wave band light in filtering environmental, makes operator can distinguish tumour viable tissue and necrotic tissue quickly and accurately;
2. bracket base of the present invention is built-in with semiconductor chilling plate, can control base temperature at 0 ~ 4 DEG C, be conducive to the maintenance of tumor tissues vigor.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the invention will be further described:
Fig. 1 is the structural representation of the discriminating device of tumour viable tissue of the present invention and necrotic tissue.
Embodiment
As shown in Figure 1, disclosed tumour viable tissue and the discriminating device of necrotic tissue, the support top 4 comprising bracket base 8 and be positioned at directly over it, described bracket base 8 is fixedly connected with by the bracket upright post 7 that erects with the edge on support top 4.Described bracket base 8 is built-in with semiconductor chilling plate 9; Lamp socket 5 is provided with below described support top, red globe lamp 6 is installed in lamp socket 5, the red light irradiation scope 10 of red globe lamp 6 can cover on bracket base 8 completely, an eyepiece frame 1 is installed at opposite side edge, support top 4, eyepiece frame is embedded with Red lightscreening plate 2, described eyepiece frame 1 is flexibly connected with support top 4 by a rotating shaft 3, the viewing angle of convenient adjustment eyepiece frame 1.
When bracket base 8 dissects tumor tissues, red globe lamp 6 launches red light, light Wavelength tunable, and intensity of light also can stepless changing, and semiconductor chilling plate 9 makes base local temperature remain on 0 ~ 4 DEG C, is convenient to the maintenance of tumor cell activity; Red lightscreening plate selects the dull and stereotyped HB10 red glass of thick 2.0mm, and it reaches 98% to ruddiness transmittance more than 600nm wavelength, is 0 to the light transmission of below 600nm, and Red lightscreening plate can the interference of other wave band light in filtering environmental.There is significant difference in healthy tissues and devitalized tissue certain specific wavelength section that is reflected in light, the size of this species diversity and organizational vitality is closely related, organizes activity higher, to Transmission light, reflection more obvious.Normal the difference of optical extinction coefficient is organized to occur in 600 ~ 860nm, 450 ~ 600nm and 280 ~ 400nm place with deactivation, wherein comparatively obvious and the part that can be clinical application is the position such as 600 ~ 800nm and 508nm.According to specific spectrum change rule, wavelength is ruddiness and infrared, far infrared part at the light of more than 630nm, and 508nm is blue light components, and the difference of this two portions optical extinction coefficient is that optical method judges normal and the most important theoretical basis of devitalized tissue's boundary.
Meanwhile, described support top can arrange red globe lamp and blue-ray light 4 times simultaneously, eyepiece frame 1 embeds simultaneously be suitable for respectively left eye, right eye viewing Red lightscreening plate and blue color filter.Whole device can be placed in aseptic operating platform and use, and is convenient to carry out aseptic technique to tumor tissues.
Above-described embodiment, only for technical conceive of the present invention and feature are described, its object is to person skilled in the art can be understood content of the present invention and implement according to this, can not limit the scope of the invention with this.All modifications done according to the spirit of main technical schemes of the present invention, all should be encompassed within protection scope of the present invention.