CN104610273B - Method of extracting gambogic acid and neogambogic acid from gamboge - Google Patents
Method of extracting gambogic acid and neogambogic acid from gamboge Download PDFInfo
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- GEZHEQNLKAOMCA-RRZNCOCZSA-N (-)-gambogic acid Chemical compound C([C@@H]1[C@]2([C@@](C3=O)(C\C=C(\C)C(O)=O)OC1(C)C)O1)[C@H]3C=C2C(=O)C2=C1C(CC=C(C)C)=C1O[C@@](CCC=C(C)C)(C)C=CC1=C2O GEZHEQNLKAOMCA-RRZNCOCZSA-N 0.000 title claims abstract description 35
- GEZHEQNLKAOMCA-UHFFFAOYSA-N epiisogambogic acid Natural products O1C2(C(C3=O)(CC=C(C)C(O)=O)OC4(C)C)C4CC3C=C2C(=O)C2=C1C(CC=C(C)C)=C1OC(CCC=C(C)C)(C)C=CC1=C2O GEZHEQNLKAOMCA-UHFFFAOYSA-N 0.000 title claims abstract description 35
- GEZHEQNLKAOMCA-GXSDCXQCSA-N gambogic acid Natural products C([C@@H]1[C@]2([C@@](C3=O)(C\C=C(/C)C(O)=O)OC1(C)C)O1)[C@H]3C=C2C(=O)C2=C1C(CC=C(C)C)=C1O[C@@](CCC=C(C)C)(C)C=CC1=C2O GEZHEQNLKAOMCA-GXSDCXQCSA-N 0.000 title claims abstract description 35
- QALPNMQDVCOSMJ-UHFFFAOYSA-N isogambogic acid Natural products CC(=CCc1c2OC(C)(CC=C(C)C)C=Cc2c(O)c3C(=O)C4=CC5CC6C(C)(C)OC(CC=C(C)/C(=O)O)(C5=O)C46Oc13)C QALPNMQDVCOSMJ-UHFFFAOYSA-N 0.000 title claims abstract description 35
- 241000598860 Garcinia hanburyi Species 0.000 title claims abstract description 31
- 229940117709 gamboge Drugs 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title abstract description 18
- BLDWFKHVHHINGR-FYJGNVAPSA-N neo-gambogic acid Chemical compound C1C2C(C)(C)OC3(C\C=C(/C)C(O)=O)C(=O)C1C=C1C(=O)C(C(O)=C4C(O)CC(OC4=C4CC=C(C)C)(C)CCC=C(C)C)=C4OC123 BLDWFKHVHHINGR-FYJGNVAPSA-N 0.000 title abstract 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 44
- 239000000284 extract Substances 0.000 claims abstract description 22
- 239000002904 solvent Substances 0.000 claims abstract description 16
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000000874 microwave-assisted extraction Methods 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 6
- 239000003208 petroleum Substances 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 239000011347 resin Substances 0.000 claims description 17
- 229920005989 resin Polymers 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 238000000605 extraction Methods 0.000 claims description 11
- 239000000047 product Substances 0.000 claims description 11
- 238000001179 sorption measurement Methods 0.000 claims description 10
- GMWTXQKKRDUVQG-WOPPDYDQSA-N 4-amino-5-bromo-1-[(2r,3s,4s,5r)-4-hydroxy-5-(hydroxymethyl)-3-methyloxolan-2-yl]pyrimidin-2-one Chemical compound C[C@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)N=C(N)C(Br)=C1 GMWTXQKKRDUVQG-WOPPDYDQSA-N 0.000 claims description 8
- 238000004064 recycling Methods 0.000 claims description 7
- 239000003463 adsorbent Substances 0.000 claims description 6
- 238000003795 desorption Methods 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000008213 purified water Substances 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 4
- 239000002250 absorbent Substances 0.000 claims description 4
- 230000002745 absorbent Effects 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 2
- 238000003786 synthesis reaction Methods 0.000 claims description 2
- 239000003814 drug Substances 0.000 abstract description 12
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 238000013375 chromatographic separation Methods 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 238000002386 leaching Methods 0.000 abstract 1
- 235000019441 ethanol Nutrition 0.000 description 12
- 239000002253 acid Substances 0.000 description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 3
- 102000011759 adducin Human genes 0.000 description 3
- 108010076723 adducin Proteins 0.000 description 3
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 230000005855 radiation Effects 0.000 description 3
- 241000345998 Calamus manan Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 208000025865 Ulcer Diseases 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- XTVYWYLMVSZOSU-LPYMAVHISA-N allogambogic acid Chemical compound O1C2(C(C3=O)(C\C=C(/C)C(O)=O)OC4(C)C)C4CC3C=C2C(=O)C2=C1C(CC=C(C)C)=C(O)C1=C2OC(CCC=C(C)C)(C)C=C1 XTVYWYLMVSZOSU-LPYMAVHISA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- 235000012950 rattan cane Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 231100000397 ulcer Toxicity 0.000 description 2
- 201000004624 Dermatitis Diseases 0.000 description 1
- 241000593508 Garcinia Species 0.000 description 1
- 235000000885 Garcinia xanthochymus Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 150000004056 anthraquinones Chemical class 0.000 description 1
- 230000000118 anti-neoplastic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000012846 chemical reference substance Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000005253 cladding Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- FPVGTPBMTFTMRT-NSKUCRDLSA-L fast yellow Chemical compound [Na+].[Na+].C1=C(S([O-])(=O)=O)C(N)=CC=C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 FPVGTPBMTFTMRT-NSKUCRDLSA-L 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000002607 hemopoietic effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000004262 preparative liquid chromatography Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000010898 silica gel chromatography Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
- C07D493/12—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains three hetero rings
- C07D493/20—Spiro-condensed systems
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention aims at providing a method of efficiently extracting and separating gambogic acid and neogambogic acid from gamboge, serving as a traditional Chinese medicine. The method comprises the following steps: crushing the gamboge, carrying out microwave extraction, and concentrating to obtain an extract A; leaching with petroleum ether, dissolving and filtering with ethanol, and carrying out chromatographic separation by virtue of a simulated moving bed to obtain a component B rich in the gambogic acid and a component C containing the gambogic acid and the neogambogic acid; and recovering a solvent, drying to obtain products, including the gambogic acid with the purity to be above 95 percent and a mixture of 10-20 percent of the gambogic acid and 30-50 percent of the neogambogic acid, in two specifications. The method of extracting the effective components of the gamboge, serving as the traditional Chinese medicine, is simple in step and high in product purity and is capable of simultaneously obtaining the products, including the gambogic acid and the neogambogic acid, in two specifications, fully extracting and separating the effective components from the raw materials and improving the production efficiency while saving the raw material resources.
Description
Technical field
The present invention relates to a kind of method extracting gambogicacid and Neo-garcinolic acid in Chinese medicine gamboge.
Background technology
Gamboge be Garcinia maingayii gamboge (garcinia hanburyi hook f.) trunk hurt after flow out glue
Shape resin, is in the cylindric or irregular block of yellowish-brown after being dried, and matter is crisp frangible, original Southeast Asia, and China some areas are such as
There is introducing and planting on the ground such as Yunnan, Guangxi, Guangdong.Gambogicacid (gambogic acid), Neo-garcinolic acid is contained in gamboge
The Multiple components such as (neomogenic acid), allogambogic acid (allogambogic acid).In clinical practice of Chinese medicine, rattan
Huang is mainly used in treating the illnesss such as swollen ulcer drug, ulcer, wet sore, tumour, stubborn dermatitis.Modern pharmacological research shows, gambogicacid is to many
Plant tumour cell and there is stronger inhibitory action, and toxic and side effect is less in effective dosage ranges, the suppression to tumour cell
There is higher selectivity, and intact animal hemopoietic system and immunologic function are not affected.There are some researches show Neo-garcinolic acid
Antitumor activity is about 2 times of gambogicacid, and being expected to exploitation is new type antineoplastic medicine.
In prior art, disclose one kind in cn101781309a using gambogicacid in supercritical fluid extraction Chinese medicine gamboge
Method with Neo-garcinolic acid.But the method need to strictly regulate and control pressure in kettle, it is difficult to adapt to the demand of industrialized production.
Cn101921283a disclose a kind of by pulverize after gamboge mix with one of silica gel, diatomite, silica flour or polyacrylamide
Load column chromatography, afford the gambogicacid extracting method of gambogicacid primary extract through methyl alcohol, ethyl acetate equal solvent.Lin Qinghua etc.
Combine to prepare Neo-garcinolic acid with preparative liquid chromatography using silica gel column chromatography, there is organic solvent and use less, avoid instead
The high cost of cladding analysis and the advantage extremely adsorbing the production loss causing, product purity can reach chemical reference substance quality requirement
(traditional Chinese medicine journal, Neo-garcinolic acid prepares liquid phase separation method, Lin Qinghua, Zhang Jian, Li Guanglei etc., vol.42, no.3,
Jun.2014, p71-72).But above two method is all to be suitable only for isolating gambogicacid or a kind of active ingredient of Neo-garcinolic acid,
Remaining active component is caused waste.
Microwave Extraction is the difference absorbing microwave ability using the material of different structure in microwave field, makes in material
Some components in some regions or extraction system are selectively heated, so that extract separates from material or system,
Enter that dielectric constant is less, in microwave absorption capacity relative mistake extractant.The advantage of this method is that extract is had relatively
High selectivity, recovery rate is high, extraction rate is fast, solvent load is few, safety, energy-conservation, equipment are simple.
Content of the invention
The present invention is intended to provide a kind of efficiently can extract the side separating gambogicacid and Neo-garcinolic acid from Chinese medicine gamboge
Method.
The method extracting gambogicacid and Neo-garcinolic acid from gamboge of the present invention, comprises the following steps:
(1) gamboge being dried is pulverized, cross 40～80 mesh sieves, be placed in microwave synthesis abstraction instrument and extracted, add second
Alcohol reflux extracts；
(2) filter out extract, after reduced pressure concentration recycling design, obtain medicinal extract a；
(3) add petroleum ether drip washing in medicinal extract a, dissolved with ethanol afterwards, filter, filtrate enters simulation and moves
Bed chromatogram carries out separating, and obtains component b rich in gambogicacid and component c containing gambogicacid, Neo-garcinolic acid；
(4) recycling design, drying under reduced pressure obtain the gambogicacid of the gambogicacid of purity more than 95% and purity 10%～20% with
The product of two kinds of specifications of Neo-garcinolic acid mixture of purity 30%～50%.
In step (1) of the present invention, during extraction, microwave power 200w～400w, 30 DEG C～70 DEG C of microwave heating temperature,
Microwave Extraction time 60s～300s, the alcohol solvent of addition and raw material solid-to-liquid ratio are 10:1～20:1.
In step (3) of the present invention, the adsorbent of SMBC filling is low pole macroporous absorbent resin,
Water wash zone is purified water, and strippant is 20～50% ethanol, and its consumption is 1～3 times of resin volume；Resin adsorption regenerated solvent
For 95% ethanol or 2～4% sodium hydrate aqueous solutions；Adsorption zone flow velocity 1～2bv/h；Water wash zone flow velocity 1～3bv/h；Desorption zone is flowed
Fast 1～3bv/h；Renewing zone flow velocity 2～3bv/h；Switching time is 600～900s；Temperature control is at 40 DEG C～60 DEG C；Pressure control
System is in 0.2mpa～0.6mpa.
Further, in step (1) of the present invention, 50 DEG C～70 DEG C of microwave heating temperature, Microwave Extraction time 100s
～200s, the alcohol solvent of addition and the liquid-solid ratio of raw material are 15:1～20:1.
Further, in step (3) of the present invention, the switching time preferably 700～800s of SMBC；Mould
The adsorbent intending mobile bed chromatic filling is ab-8 or dm130；Strippant consumption is preferably 3 times of resin volume.
Although simulated moving bed technology can apply to natural products or the separating-purifying of medicine has had altogether in the art
Know, but because Simulation moving bed big be structurally characterized in that by chemical industry continuous, countercurrently, rectifying, the key element such as backflow all concentrate into
System, it is a multiple degrees of freedom, dynamic, be mutually related nonlinear system.Therefore, for its prediction, optimize and control
It is more complicated problem, in particular for a certain natural products kind, the prediction of separation parameter is a sufficiently complex problem.
Reported microwave radiation exaraction is mainly used in extraction alkaloids, Anthraquinones, flavonoids, saponins, polysaccharide, volatilization
The traditional Chinese medicine ingredients such as oil, pigment.But the extraction specific to active ingredient in gamboge, whether microwave radiation exaraction adapts to, and its phase
The determination closing technological parameter is still a complicated process.
Chinese medicine gamboge extraction process of effective component of the present invention, using microwave radiation exaraction and SMBC
, it is determined that related process parameters, simply, product purity is higher, can obtain two simultaneously for step for the technique that method separating-purifying combines
Plant gambogicacid and the Neo-garcinolic acid product of specification, the active ingredient in raw material is fully extracted and separates, saved raw material resources
Also improve production efficiency simultaneously.
Specific embodiment
Embodiment 1
The Gamboge (gamboge acid content 31.28%, Neo-garcinolic acid content 12.34%) that 200g is dried crosses 40 mesh after pulverizing
Sieve, adds Extraction solvent absolute ethyl alcohol, Microwave Extraction 2min under the conditions of power 250w, temperature 50 C with solid-to-liquid ratio 1:20.Cross
Leach extract, after reduced pressure concentration recycling design, obtain medicinal extract a；Add appropriate petroleum ether drip washing in medicinal extract a, add 95%
Ethanol is dissolved, and filters, and filtrate enters SMBC and carries out separating.Adsorbent is ab-8 macroporous absorbent resin, solution
Vapor is 25% ethanol water, and its consumption is 3 times of resin volume, and water wash zone is purified water, and resin adsorption regenerated solvent is
95% ethanol, adsorption zone flow velocity 2bv/h, desorption zone flow velocity 3bv/h, water wash zone flow velocity 3bv/h, renewing zone flow velocity 3bv/h, switching
Time is 700s, temperature 50 C, pressure 0.5mpa.Separate component b obtaining rich in gambogicacid and contain gambogicacid, Neo-garcinolic acid
Component c.Component b and c are separately recovered solvent, drying under reduced pressure, respectively obtain gamboge acid content 96.1% product 33.3g and
Gamboge acid content 13.4%, the product 15.9g of Neo-garcinolic acid content 48.2%.
Embodiment 2
The Gamboge (gamboge acid content 31.28%, Neo-garcinolic acid content 12.34%) that 500g is dried crosses 80 mesh after pulverizing
Sieve, adds Extraction solvent absolute ethyl alcohol, Microwave Extraction 2.5min under the conditions of power 300w, temperature 70 C with solid-to-liquid ratio 1:15.
Filter out extract, after reduced pressure concentration recycling design, obtain medicinal extract a；Add appropriate petroleum ether drip washing in medicinal extract a, add
95% ethanol is dissolved, and filters, and filtrate enters SMBC and carries out separating.Adsorbent is dm130 macroporous absorption tree
Fat, strippant is 30% ethanol water, and its consumption is 3 times of resin volume, and water wash zone is purified water, and resin adsorption regeneration is molten
Agent is 95% ethanol, adsorption zone flow velocity 2bv/h, desorption zone flow velocity 3bv/h, water wash zone flow velocity 3bv/h, renewing zone flow velocity 3bv/h,
Switching time is 750s, temperature 50 C, pressure 0.5mpa.Separate component b obtaining rich in gambogicacid and contain gambogicacid, new rattan
Component c of yellow acid.Component b and c are separately recovered solvent, drying under reduced pressure, respectively obtain the product of gamboge acid content 95.8%
The 82.9g and product 44.3g of gamboge acid content 11.2%, Neo-garcinolic acid content 44.6%.
Embodiment 3
The Gamboge (gamboge acid content 31.28%, Neo-garcinolic acid content 12.34%) that 600g is dried crosses 60 mesh after pulverizing
Sieve, adds Extraction solvent absolute ethyl alcohol, Microwave Extraction 3min under the conditions of power 250w, temperature 70 C with solid-to-liquid ratio 1:20.Cross
Leach extract, after reduced pressure concentration recycling design, obtain medicinal extract a；Add appropriate petroleum ether drip washing in medicinal extract a, add 95%
Ethanol is dissolved, and filters, and filtrate enters SMBC and carries out separating.Adsorbent is ab-8 macroporous absorbent resin, solution
Vapor is 30% ethanol water, and its consumption is 3 times of resin volume, and water wash zone is purified water, and resin adsorption regenerated solvent is
95% ethanol, adsorption zone flow velocity 2bv/h, desorption zone flow velocity 3bv/h, water wash zone flow velocity 3bv/h, renewing zone flow velocity 3bv/h, switching
Time is 750s, temperature 50 C, pressure 0.5mpa.Separate component b obtaining rich in gambogicacid and contain gambogicacid, Neo-garcinolic acid
Component c.Component b and c are separately recovered solvent, drying under reduced pressure, respectively obtain gamboge acid content 96.4% product 98.3g and
Gamboge acid content 12.6%, the product 52.1g of Neo-garcinolic acid content 45.2%.
Claims (1)
1. a kind of method of extraction gambogicacid and Neo-garcinolic acid from gamboge is it is characterised in that comprise the following steps:
(1) gamboge being dried is pulverized, cross 40～80 mesh sieves, be placed in microwave synthesis abstraction instrument and extracted, add ethanol to return
Stream extracts；During extraction, microwave power 200w～400w, 50 DEG C～70 DEG C of microwave heating temperature, Microwave Extraction time 100s～
200s, the alcohol solvent of addition and raw material solid-to-liquid ratio are 15:1～20:1；
(2) filter out extract, after reduced pressure concentration recycling design, obtain medicinal extract a；
(3) add petroleum ether drip washing in medicinal extract a, dissolved with ethanol afterwards, filter, filtrate enters SMBC
Carry out separating, obtain component b rich in gambogicacid and component c containing gambogicacid, Neo-garcinolic acid；SMBC is filled
Adsorbent be low pole macroporous absorbent resin ab-8 or dm130, water wash zone be purified water, strippant be 20～50% ethanol,
Its consumption is 1～3 times of resin volume；Resin adsorption regenerated solvent is 95% ethanol or 2～4% sodium hydrate aqueous solutions, uses
Measure 3 times for resin volume；Adsorption zone flow velocity 1～2bv/h；Water wash zone flow velocity 1～3bv/h；Desorption zone flow velocity 1～3bv/h；Again
Raw area flow velocity 2～3bv/h；Switching time is 700～800s；Temperature control is at 40 DEG C～60 DEG C；Stress control 0.2mpa～
0.6mpa；
(4) recycling design, drying under reduced pressure obtain the gambogicacid of purity more than 95% and the gambogicacid of purity 10%～20% with pure
The product of two kinds of specifications of Neo-garcinolic acid mixture of degree 30%～50%.
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| CN107137392A (en) * | 2017-06-02 | 2017-09-08 | 南方医科大学 | Neo-garcinolic acid is preparing the application in suppressing hepatoma cell proliferation, migration and liver cancer apoptosis reducing medicine |
| CN110229169A (en) * | 2019-06-27 | 2019-09-13 | 济宁医学院 | A kind of method Neo-garcinolic acid separation and its prepare the new gamboge amide of N- aryl |
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| CN102617590A (en) * | 2012-03-07 | 2012-08-01 | 广州牌牌生物科技有限公司 | Preparation process for neogambogic acid |
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