CN104607458A - Method for restoring chemically contaminated soil through Klebsiella pneumoniae - Google Patents
Method for restoring chemically contaminated soil through Klebsiella pneumoniae Download PDFInfo
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- CN104607458A CN104607458A CN201510018800.0A CN201510018800A CN104607458A CN 104607458 A CN104607458 A CN 104607458A CN 201510018800 A CN201510018800 A CN 201510018800A CN 104607458 A CN104607458 A CN 104607458A
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Abstract
The invention relates to a method for restoring chemically contaminated soil through Klebsiella pneumonia. The method comprises steps as follows: a solution containing the Klebsiella pneumonia is cultured; the concentration of the Klebsiella pneumonia in the solution is 109-1,014 pieces per milliliter; the solution is sprayed onto the chemically contaminated soil at the standard of 5-10 liters per cubic meter; the soil is left to stand for 10-100 days after being stirred sufficiently. According to the method for restoring the chemically contaminated soil through the Klebsiella pneumonia, organic pollutants in the chemically contaminated soil are decomposed with the Klebsiella pneumonia, so that the soil can restore an original state, and harm to human health is avoided.
Description
Technical field
The present invention relates to contaminated soil recovery technology field, particularly a kind of Friedlander's bacillus method that the soil being subject to chemical pollution is recovered.
Background technology
Chemical industry carries out chemical treatment to the various resources in environment and transforms the production division of processing, it is diversified that its product and discarded object chemically form, and quantity is also quite large, these discarded object content are harmful when finite concentration mostly, have plenty of extremely toxic substance, entered environment will pollute.Some chemical products in use can cause again some to pollute, and the pollution even caused than production itself is even more serious, more extensive.The industrial pollution of China accounts for 70% in environmental pollution.Develop rapidly along with industrial, the control of industrial pollution more and more causes the extensive attention of people.
In addition on the one hand, Klebsiella (Klebsiella) is Gram-negative bacteria, shaft-like, which includes the common Friedlander's bacillus of human infection's disease (Klebsiella pneumoniae).Klebsiella is at ubiquity in environment.The pod membrane that Klebsiella has the polysaccharide of a large amount of stickiness to be formed usually is coated.They can the mankind, and the individuality that particularly immunity is low causes pneumonia, urinary tract infections, and bacteremia etc. infect disease.Wherein Friedlander's bacillus is pathogenic comparatively strong to people, is one of important conditioned pathogen and hospital-acquired infection bacterium.
Summary of the invention
The present invention will solve large, the unmanageable technical problem of chemical pollution harm in prior art, provides a kind of method that Friedlander's bacillus recovers the soil being subject to chemical pollution.
In order to solve the problems of the technologies described above, technical scheme of the present invention is specific as follows:
The method that Friedlander's bacillus recovers the soil being subject to chemical pollution, comprises the following steps:
Cultivate the solution containing Friedlander's bacillus; The concentration of the Friedlander's bacillus in this solution is 10
9~ 10
14individual every milliliter;
Spray the soil being subject to chemical pollution with described solution, the soil of every cubic metre sprays 5 ~ 10 liters;
After soil is fully stirred, place 10 ~ 100 day time.
In technique scheme, the temperature and humidity condition between soil resting period is: humidity is 60 ~ 100%, and temperature is 30 ~ 40 degrees Celsius.
The present invention has following beneficial effect:
The method that Friedlander's bacillus of the present invention recovers the soil being subject to chemical pollution, utilizes Friedlander's bacillus to decompose the organic pollution be subject in the soil of chemical pollution, makes it restore to the original state, and avoids harm humans healthy.
Detailed description of the invention
Invention thought of the present invention is: the method that Friedlander's bacillus of the present invention recovers the soil being subject to chemical pollution, for Friedlander's bacillus, a kind of new application mode is proposed, Friedlander's bacillus is utilized to decompose the organic pollution be subject in the soil of chemical pollution, make it restore to the original state, avoid harm humans healthy.
Below the present invention is described in detail.
Embodiment 1
The method that Friedlander's bacillus of the present invention recovers the soil being subject to chemical pollution, comprises the following steps:
Cultivate the solution containing Friedlander's bacillus; The concentration of the Friedlander's bacillus in this solution is 10
9individual every milliliter;
Spray the soil being subject to chemical pollution with described solution, the soil of every cubic metre sprays 10 liters;
After soil is fully stirred, place 100 day time; Temperature and humidity condition between soil resting period is: humidity is 60 ~ 100%, and temperature is 30 ~ 40 degrees Celsius.
Embodiment 2
The method that Friedlander's bacillus of the present invention recovers the soil being subject to chemical pollution, comprises the following steps:
Cultivate the solution containing Friedlander's bacillus; The concentration of the Friedlander's bacillus in this solution is 10
14individual every milliliter;
Spray the soil being subject to chemical pollution with described solution, the soil of every cubic metre sprays 5 liters;
After soil is fully stirred, place 10 day time; Temperature and humidity condition between soil resting period is: humidity is 60 ~ 100%, and temperature is 30 ~ 40 degrees Celsius.
Embodiment 3
The method that Friedlander's bacillus of the present invention recovers the soil being subject to chemical pollution, comprises the following steps:
Cultivate the solution containing Friedlander's bacillus; The concentration of the Friedlander's bacillus in this solution is 10
12individual every milliliter;
Spray the soil being subject to chemical pollution with described solution, the soil of every cubic metre sprays 8 liters;
After soil is fully stirred, place 60 day time; Temperature and humidity condition between soil resting period is: humidity is 60 ~ 100%, and temperature is 30 ~ 40 degrees Celsius.
Obviously, above-described embodiment is only for clearly example being described, and the restriction not to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.And thus the apparent change of extending out or variation be still among the protection domain of the invention.
Claims (2)
1. by the method that Friedlander's bacillus recovers the soil being subject to chemical pollution, it is characterized in that, comprise the following steps:
Cultivate the solution containing Friedlander's bacillus; The concentration of the Friedlander's bacillus in this solution is 10
9~ 10
14individual every milliliter;
Spray the soil being subject to chemical pollution with described solution, the soil of every cubic metre sprays 5 ~ 10 liters;
After soil is fully stirred, place 10 ~ 100 day time.
2. method according to claim 1, is characterized in that, the temperature and humidity condition between soil resting period is: humidity is 60 ~ 100%, and temperature is 30 ~ 40 degrees Celsius.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510018800.0A CN104607458A (en) | 2015-01-14 | 2015-01-14 | Method for restoring chemically contaminated soil through Klebsiella pneumoniae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510018800.0A CN104607458A (en) | 2015-01-14 | 2015-01-14 | Method for restoring chemically contaminated soil through Klebsiella pneumoniae |
Publications (1)
| Publication Number | Publication Date |
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| CN104607458A true CN104607458A (en) | 2015-05-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510018800.0A Pending CN104607458A (en) | 2015-01-14 | 2015-01-14 | Method for restoring chemically contaminated soil through Klebsiella pneumoniae |
Country Status (1)
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Citations (9)
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|---|---|---|---|---|
| EP0481963A2 (en) * | 1986-07-03 | 1992-04-22 | Occidental Chemical Corporation | Microorganisms for degrading toxic waste materials |
| CN1370227A (en) * | 1999-08-20 | 2002-09-18 | 李性器 | Bacterial consortium EBC1000 and method using bacterial consortium EBC 1000 for remedying biologically recalcitrant toxic chemicals contained in industrial wastewater etc. |
| CN101033453A (en) * | 2006-03-07 | 2007-09-12 | 中国科学院过程工程研究所 | Klebsiella and application of the same for eliminating organic nitrogen in fossil fuels |
| KR101018026B1 (en) * | 2010-07-16 | 2011-03-02 | (주)동명엔터프라이즈 | Microbial preparations for the remediation of a soil with contaminated organic compounds, process for production thereof, and method for decontaminating the oilpollution of soil |
| CN102847705A (en) * | 2012-05-30 | 2013-01-02 | 华南农业大学 | Restoration method for degrading soil PCP residue through cooperation of earthworms and microbes |
| CN102943052A (en) * | 2012-11-22 | 2013-02-27 | 台州职业技术学院 | Heavy metal-resistant polycyclic aromatic hydrocarbon (PAHs) degrading bacteria and application thereof in remediation of composite contaminated soil |
| CN103350105A (en) * | 2013-06-26 | 2013-10-16 | 深圳清华大学研究院 | Method for associating plants and microorganisms to accumulate heavy metal cadmium in soil and application thereof |
| CN103484393A (en) * | 2013-06-04 | 2014-01-01 | 四川大学 | Klebsiella inoculum capable of biodegrading residual chlorpyrifos pesticide on surfaces of garden stuffs and soil |
| CN104259195A (en) * | 2014-09-29 | 2015-01-07 | 镇江华域环保设备制造有限公司 | Contaminated soil repair method |
-
2015
- 2015-01-14 CN CN201510018800.0A patent/CN104607458A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0481963A2 (en) * | 1986-07-03 | 1992-04-22 | Occidental Chemical Corporation | Microorganisms for degrading toxic waste materials |
| CN1370227A (en) * | 1999-08-20 | 2002-09-18 | 李性器 | Bacterial consortium EBC1000 and method using bacterial consortium EBC 1000 for remedying biologically recalcitrant toxic chemicals contained in industrial wastewater etc. |
| CN101033453A (en) * | 2006-03-07 | 2007-09-12 | 中国科学院过程工程研究所 | Klebsiella and application of the same for eliminating organic nitrogen in fossil fuels |
| CN101033453B (en) * | 2006-03-07 | 2010-07-14 | 中国科学院过程工程研究所 | Klebsiella and application of the same for eliminating organic nitrogen in fossil fuels |
| KR101018026B1 (en) * | 2010-07-16 | 2011-03-02 | (주)동명엔터프라이즈 | Microbial preparations for the remediation of a soil with contaminated organic compounds, process for production thereof, and method for decontaminating the oilpollution of soil |
| CN102847705A (en) * | 2012-05-30 | 2013-01-02 | 华南农业大学 | Restoration method for degrading soil PCP residue through cooperation of earthworms and microbes |
| CN102943052A (en) * | 2012-11-22 | 2013-02-27 | 台州职业技术学院 | Heavy metal-resistant polycyclic aromatic hydrocarbon (PAHs) degrading bacteria and application thereof in remediation of composite contaminated soil |
| CN103484393A (en) * | 2013-06-04 | 2014-01-01 | 四川大学 | Klebsiella inoculum capable of biodegrading residual chlorpyrifos pesticide on surfaces of garden stuffs and soil |
| CN103350105A (en) * | 2013-06-26 | 2013-10-16 | 深圳清华大学研究院 | Method for associating plants and microorganisms to accumulate heavy metal cadmium in soil and application thereof |
| CN104259195A (en) * | 2014-09-29 | 2015-01-07 | 镇江华域环保设备制造有限公司 | Contaminated soil repair method |
Non-Patent Citations (1)
| Title |
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| 刘晓艳等: "《土壤中石油类污染物的迁移与修复治理技术》", 31 January 2014, 上海交通大学出版社 * |
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Application publication date: 20150513 |