CN104606713B - Three-dimensional parallel collagenous fiber-silk bracket as well as preparation method and application thereof - Google Patents
Three-dimensional parallel collagenous fiber-silk bracket as well as preparation method and application thereof Download PDFInfo
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- CN104606713B CN104606713B CN201510005188.3A CN201510005188A CN104606713B CN 104606713 B CN104606713 B CN 104606713B CN 201510005188 A CN201510005188 A CN 201510005188A CN 104606713 B CN104606713 B CN 104606713B
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Abstract
The invention provides a three-dimensional parallel collagenous fiber-silk bracket as well as a preparation method thereof. The bracket is formed by a woven silk base frame and a parallel collagenous fiber layer which coats on the upper and lower surfaces of the woven silk base frame, wherein the woven silk base frame adopts a mesh structure which is woven by silk and has a mesh size of 0.25-25 mm<2>; the pore diameter of the parallel collagenous fiber layer is 10-300 microns; the woven silk is sericin-removed woven silk; the bracket can be used as a biological bracket and applied to the repair of muscle tendons/ligaments. The three-dimensional parallel collagenous fiber-silk bracket provided by the invention is simple to prepare, good in biocompatibility and good in mechanical property, has a topological structure similar to muscle tendons/ligaments and can be used for promoting the surface grown stem cells to differentiate toward the muscle tendons/ligaments and promoting the regeneration of the muscle tendons/ligaments.
Description
(1) technical field
The present invention relates to a kind of three-dimensional timbering material for tendon/ligament repair and preparation method thereof, more particularly to
A kind of three-dimensional parallel collagen fiber-silk stent and its preparation method and application.
(2) background technology
With China's expanding economy and the raising of living standards of the people, the concept of health of people is also being lifted, for right
The yearning of healthy physique, compatriots participate in physical training and the quantity of competition substantially to be increased, and athletic injury is also more and more, its
Middle ligament/tendon injury accounts for more than 50%.There is data to suggest that, per 200,000,000 populations in 1 year at least up to ten million tendon injury disease
Example.
Currently, clinically mainly by directly suture, autotransplantation, allograft, xenotransplantation and prosthetic material are repaiied
Answer art to repair ligament and tendon injury.However, these operative reconstruction regeneration techniqueses have its intrinsic defect, such as recurrent
Tear, donor shortage, donor site complication, immunologic rejection and poor support conformability.
At present the shortcoming of clinical method promotes the research steering degradable biomaterial and organizational project of tendon/ligament repair
Method.By researcher using macromolecular material fiber or silkworm silk constitute pigtail support for repair damage tendon, however, these
Pigtail support does not have internal voids, it is impossible to carry a large amount of cells, body can not be allowed to be formed and be connected with each other functional new organization.
Having researcher to be inoculated with a large amount of cells using collagen in addition is used for tendon/ligament tissue engineering.These supports solve cell tissue
Receiving problem, but their mechanical property is very poor, it is impossible to bear the physiology mechanical force of body, it is difficult to be applied to clinic.
There is research that silkworm silk and collagen sponge are combined with each other before, constructed the tendon/ligament tissue of a practicality
Engineering rack.Collagen sponge provides repair cell adhesion and the space for growing into, and it is strong that braided silk fiber provides enough mechanics
Degree.But it differs greatly in structural arrangement with the shape of tendon/ligament tissue, the 26S Proteasome Structure and Function of tendon/ligament is repaired
It is also to differ greatly compared with normal tendon/ligament.One kind is preferable, can attract aim cell in damage location collection coalescence
The combination of silk stent for having biological activity that cell migration can effectively be guided still suffers from huge challenge.
(3) content of the invention
It is an object of the present invention to provide a kind of three-dimensional parallel collagen fiber-silk stent and preparation method and application, this
Bright support has good mechanical property, using the teaching of the invention it is possible to provide the space that repair cell is adhered to and grown into, with similar to tendon/ligament
Structure, can effectively guide cell migration and arrangement, the reparation of tendon/ligament can be applied to as biological support.
The technical solution used in the present invention is：
A kind of three-dimensional parallel collagen fiber-silk stent, the support is by weaving silkworm silk pedestal and be coated on braiding silkworm silk
The parallel collagen fiber layer on pedestal upper and lower surface is constituted, and described braiding silkworm silk pedestal is to be woven into mesh size by silkworm silk
For 0.25～25mm2Mesh sheet structure, the pore diameter size of the parallel collagen fiber is 10～300um；Described braiding
Silkworm silk is the braiding silkworm silk for eliminating sericin.
The three-dimensional parallel collagen fiber-silk stent of the present invention, the mesh size of preferably described braiding silkworm silk pedestal is 1～
9mm2。
It is preferred that the pore diameter size of the parallel collagen fiber is 50～150um.
Present invention also offers a kind of preparation method of the three-dimensional parallel collagen fiber-silk stent, described preparation
Method comprises the steps：
(1) braiding silkworm silk pedestal is removed into sericin；
(2) in the top of the braiding silkworm silk pedestal for eliminating sericin, lower section concentration is injected separately into for 5～20mg/ml
Collagen solution, and make braiding silkworm silk pedestal both sides collagen solution layer thickness be 1～10mm；
(3) sub-cooled makes collagen set, and then vacuum decompression is dried；
(4) dehydrothermal is finally carried out, obtains described three-dimensional parallel collagen fiber-silk stent.
In step (1) of the present invention, the braiding silkworm silk pedestal removes the method for sericin can be selected from one of following：Carbon
Acid sodium solution boils removal method, detergent and boils removal method, boric acid and boils removal method or directly heat removal method etc., and these go
Except the method for sericin, the present invention is suitable for.
Specifically, described sodium carbonate liquor boils removal method and is：Braiding silkworm silk pedestal is immersed in into 0.2wt%Na2CO3
In aqueous solution, 60～150min is boiled, water 2～5 times is changed in centre, room temperature or drying baker are dried, obtain final product and eliminate sericin
Braiding silkworm silk pedestal.
Specifically, the step (2) is carried out as follows：First, the mould of temperature transfer bonding jumper is embedded with one end
Implantation concentration is the collagen solution of 5～20mg/ml in tool, and makes sample path length for 1～10mm, flat in solution upper surface after paving
The braiding silkworm silk pedestal for eliminating sericin is put, then implantation concentration is the glue of 5～20mg/ml above braiding silkworm silk pedestal
Original solution, and make sample path length for 1～10mm, pave；Described temperature transfer bonding jumper selected from aluminum strip, stainless steel strip or
The good material of the transmission temperature performance such as copper bar, the material of the mould is selected from the heat conductivility difference such as acrylic, ceramics or silica gel
Material.
In step (3) of the present invention, the sub-cooled method of collagen set is allow using one of following：Bubble equipped with liquid nitrogen
Foam box, refrigeration machine or low temperature ice cube etc., these manufacture thermogrades and the method for making collagen set, are suitable for the present invention.
It is concrete to recommend the three-dimensional parallel collagen fiber-silk stent of the present invention to prepare as follows：
(1) braiding silkworm silk pedestal is immersed in into 0.2wt%Na2CO3In aqueous solution, 60～150min is boiled, water 2 is changed in centre
～5 times, room temperature or drying baker are dried, and obtain eliminating the braiding silkworm silk pedestal of sericin；
(2) it is the collagen solution of 5～20mg/ml to be embedded with implantation concentration in the acrylic mould of aluminum strip at one end, and is made
Sample path length is 1～10mm, and the braiding silkworm silk base that step (1) gained eliminates sericin is kept flat in solution upper surface after paving
Frame, then implantation concentration is the collagen solution of 5～20mg/ml above braiding silkworm silk pedestal, and make sample path length for 1～
10mm, paves；
(3) other end of acrylic mould is connected with the foam box equipped with liquid nitrogen, adjusts the distance of foam box and mould
For 10～100mm, after collagen all solidification, it is placed in vacuum and drains in machine and drain；
(4) dehydrothermal is finally carried out, method is：<In the vacuum drying oven of 10mbar, 22 DEG C are placed one day, 110 DEG C
Place three days, 65 DEG C are placed one day, obtain final product described three-dimensional parallel collagen fiber-silk stent.
The three-dimensional parallel collagen fiber-silk stent of the present invention can be applied to the reparation of tendon/ligament as biological support.
Compared with prior art, the beneficial effects are mainly as follows：
1st, the three-dimensional parallel collagen fiber-silk stent manufacture method of the present invention is simple, and its shape can be according to mould control
Adjustment, its hole can be adjusted by temperature and collagen concentration；
2nd, the three-dimensional parallel collagen fiber-silk stent good biocompatibility of the present invention, collagen and silkworm silk can be in organisms
Inside it is degraded；
3rd, the three-dimensional parallel collagen fiber-silk stent mechanical property of the present invention is good, needed for can meeting tendon/ligament
Powerful mechanics；
4th, the topological structure of the three-dimensional parallel collagen fiber-silk stent of the present invention is similar to tendon/ligament, can promote
Its epontic stem cell promotes tendon/ligament regeneration to tendon/ligament differentiation.
(4) illustrate
Fig. 1：Braiding silkworm silk substantially picture；
Fig. 2：Three-dimensional parallel collagen fiber-silk stent is substantially schemed；
Fig. 3：Parallel collagen fiber electron-microscope scanning figure, Fig. 3-1 be 6mg/ml collagens, Fig. 3-2 be 10mg/ml collagens, Fig. 3-3
For 18mg/ml collagens；
Fig. 4：Propagation of the tendon stem cell/precursor on three-dimensional parallel collagen fiber-silk stent；
Fig. 5：HE is dyeed after rabbit rotator cuff injury is repaired 4 weeks, and A is disorderly collagen fiber-silk stent group, and B puts down for three-dimensional
Row collagen fiber-silk stent group；
Fig. 6：Masson is dyeed after rabbit rotator cuff injury is repaired 4 weeks, and A is disorderly collagen fiber-silk stent group, and B is three
Tie up parallel collagen fiber-silk stent group；
Fig. 7：Rabbit rotator cuff injury newborn tendon collagen content after repairing 4 weeks；
Fig. 8：Rabbit rotator cuff injury repair 4 weeks after tendon related gene expression.
(5) specific embodiment
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This：
Embodiment 1
It is 1mm by mesh size2Braiding silkworm silk remove sericin, be cut into 2*2cm (see Fig. 1).Under braiding silkworm silk
The collagen of side injection 2mm thick 6mg/ml, injects the collagen of the thick 6mg/ml of 3mm above braiding silkworm silk.Adjustment dress liquid nitrogen
Foam box is 5cm with the distance of mould.Vacuum is placed in after solidification drains in machine and drain, then carry out dehydrothermal, obtain hole
The three-dimensional parallel collagen fiber-silk stent of diameter about 200um.Observe its electron-microscope scanning picture (see Fig. 3-1).
Embodiment 2
It is 1mm by mesh size2Braiding silkworm silk remove sericin, be cut into 2*2cm.Inject below braiding silkworm silk
The collagen of 2mm thick 10mg/ml, injects the collagen of the thick 10mg/ml of 3mm above braiding silkworm silk.The foam of adjustment dress liquid nitrogen
Box is 5cm with the distance of collagen, silkworm silk.Vacuum is placed in after solidification drains in machine and drain, then carry out dehydrothermal, obtain hole
The three-dimensional parallel collagen fiber-silk stent (see Fig. 2) of diameter about 80um.Observe its electron-microscope scanning picture (see Fig. 3-
2)。
Embodiment 3
It is 1mm by mesh size2Braiding silkworm silk remove sericin, be cut into 2*2cm.Inject below braiding silkworm silk
The collagen of 2mm thick 18mg/ml, injects the collagen of the thick 18mg/ml of 3mm above braiding silkworm silk.The foam of adjustment dress liquid nitrogen
Box is 5cm with the distance of collagen, silkworm silk.Vacuum is placed in after solidification drains in machine and drain, then carry out dehydrothermal, obtain hole
The three-dimensional parallel collagen fiber-silk stent of diameter about 20um.Observe its electron-microscope scanning picture (see Fig. 3-3).
Embodiment 4
Plant tendon stem cell/precursor on the support of gained in example 2.By cck-8 test kits detection 1,
3rd, the proliferative conditions (see Fig. 4) of 7 days cells, show cell normal proliferation on three-dimensional parallel collagen fiber-silk stent.
Embodiment 5
Application of the three-dimensional parallel collagen fiber-silk stent in rotator cuff injury.
(1) animal model is set up：By the female rabbits of 5 about 2500g, after intravenous anesthesia, cut skin, fascia and
Part muscle, supraspinatuss tendon is fully exposed, and is pressing close to cut-out supraspinatuss tendon at greater tubercles of humerus.
(2) stenter to implant：Same rabbit or so control, experimental group is the support of gained in embodiment 2, the glue of matched group
Original content and silkworm silk are distributed with embodiment 2, directly put -20 DEG C of refrigerator solidifications, and lyophilizing, crosslinking are with embodiment 2.Support one end is straight
Connect and the suture of the supraspinatuss tendon broken ends of fractured bone, the other end is connected by osseous tunnel with greater tubercles of humerus.
(3) tissue repairing ability：Postoperative 4 weeks, rabbit supraspinatuss tendon is taken out, carry out HE dyeing, Masson dyeing, collagen
Assay and tendon related gene are detected.
HE coloration results：Experimental group internal stent also has newborn tendon to grow into, and the newborn tendon in crt bracket inside grows into
Seldom (see Fig. 5).As a result show that three-dimensional parallel collagen fiber-silk stent can promote tendon to regenerate.
Masson coloration results：The newborn tendon of experimental group internal stent is more more than matched group, more ripe (see Fig. 6).Knot
Fruit shows that the facilitation of three-dimensional parallel collagen fiber-silk stent tendon regeneration is stronger than disorderly collagen fiber-silk stent.
Contents of collagen:The collagen content of experimental group be (131.12 ± 36.73) mg/g, matched group for (121.32 ±
27.12) mg/g (see Fig. 7), shows that three-dimensional parallel collagen fiber-silk stent can promote more collagenation, promotes flesh
Tendon regenerates.
Tendon related gene expression：Col1, Col3, Tnc, Bgn have higher expression in experimental group (see Fig. 8).Show
Three-dimensional parallel collagen fiber-silk stent can promote tendon related gene expression, so as to promote tendon to regenerate.
Claims (8)
1. a kind of three-dimensional parallel collagen fiber-silk stent, it is characterised in that the support is by weaving silkworm silk pedestal and cladding
Parallel collagen fiber layer on braiding silkworm silk pedestal upper and lower surface is constituted, and described braiding silkworm silk pedestal is woven by silkworm silk
It is 0.25～25mm into mesh size2Mesh sheet structure, the pore diameter size of the parallel collagen fiber is 10～300um；
Described braiding silkworm silk is the braiding silkworm silk for eliminating sericin；
Described three-dimensional parallel collagen fiber-silk stent is prepared as follows obtaining：
(1) braiding silkworm silk pedestal is removed into sericin；
(2) first, the collagen solution that implantation concentration in the mould of temperature transfer bonding jumper is 5～20mg/ml is embedded with one end,
And sample path length is made for 1～10mm, the braiding silkworm silk pedestal for eliminating sericin is kept flat in solution upper surface after paving, then
Implantation concentration is the collagen solution of 5～20mg/ml above braiding silkworm silk pedestal, and makes sample path length for 1～10mm, is paved i.e.
Can；Described temperature transfer bonding jumper is selected from aluminum strip, stainless steel strip or copper bar, and the material of the mould is selected from acrylic, ceramics
Or silica gel；
(3) sub-cooled makes collagen set, and then vacuum decompression is dried；
(4) dehydrothermal is finally carried out, obtains described three-dimensional parallel collagen fiber-silk stent.
2. three-dimensional parallel collagen fiber-silk stent as claimed in claim 1, it is characterised in that the braiding silkworm silk pedestal
Mesh size be 1～9mm2。
3. three-dimensional parallel collagen fiber-silk stent as claimed in claim 1, it is characterised in that the parallel collagen fiber
Pore diameter size be 50～150um.
4. three-dimensional parallel collagen fiber-silk stent as claimed in claim 1, it is characterised in that in step (1), the volume
Knit silkworm silk pedestal and remove the method for sericin selected from one of following：Sodium carbonate liquor boils removal method, detergent and boils removal
Method, boric acid boil removal method or directly heat removal method.
5. three-dimensional parallel collagen fiber-silk stent as claimed in claim 4, it is characterised in that described sodium carbonate liquor
Boiling removal method is：Braiding silkworm silk pedestal is immersed in into 0.2wt%Na2CO3In aqueous solution, 60～150min is boiled, water is changed in centre
2～5 times, room temperature or drying baker are dried, and obtain final product the braiding silkworm silk pedestal for eliminating sericin.
6. three-dimensional parallel collagen fiber-silk stent as claimed in claim 1, it is characterised in that in the step (3), make
The sub-cooled method of collagen set is using one of following：Foam box equipped with liquid nitrogen, refrigeration machine or low temperature ice cube.
7. three-dimensional parallel collagen fiber-silk stent as claimed in claim 1, it is characterised in that described three-dimensional parallel glue
Fibril-silk stent is prepared as follows obtaining：
(1) braiding silkworm silk pedestal is immersed in into 0.2wt%Na2CO3In aqueous solution, 60～150min is boiled, water 2～5 is changed in centre
Secondary, room temperature or drying baker are dried, and obtain eliminating the braiding silkworm silk pedestal of sericin；
(2) it is the collagen solution of 5～20mg/ml to be embedded with implantation concentration in the acrylic mould of aluminum strip at one end, and makes solution
Thickness is 1～10mm, and the braiding silkworm silk pedestal that step (1) gained eliminates sericin is kept flat in solution upper surface after paving,
Then implantation concentration is the collagen solution of 5～20mg/ml above braiding silkworm silk pedestal, and makes sample path length for 1～10mm, paving
It is flat；
(3) other end of acrylic mould is connected with the foam box equipped with liquid nitrogen, it is 10 to adjust foam box with the distance of mould
～100mm, after collagen all solidification, is placed in vacuum and drains in machine and drain；
(4) dehydrothermal is finally carried out, method is：<In the vacuum drying oven of 10mbar, 22 DEG C are placed one day, 110 DEG C of placements
Three days, 65 DEG C were placed one day, obtain final product described three-dimensional parallel collagen fiber-silk stent.
8. the three-dimensional parallel collagen fiber-silk stent as described in one of claim 1～7 is applied to flesh as biological support
The reparation of tendon/ligament.
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108653811B (en) * | 2017-03-28 | 2021-03-12 | 上海微创医疗器械（集团）有限公司 | Artificial ligament and preparation method thereof |
| CN107469147A (en) * | 2017-08-14 | 2017-12-15 | 浙江大学 | A kind of silk collagen scaffold of biotic factor release and preparation method and application |
| CN116510084A (en) * | 2018-12-29 | 2023-08-01 | 浙江星月生物科技股份有限公司 | Woven silk bracket with adjustable mechanical degradation performance and preparation method and application thereof |
| CN110947032A (en) * | 2019-12-16 | 2020-04-03 | 浙江大学 | Silk/PET mixed-woven bracket and preparation method and application thereof |
| EP4096729A4 (en) * | 2020-01-31 | 2024-03-06 | Embody Inc | Braided surgical implants |
| CN113944003B (en) * | 2020-10-28 | 2024-01-26 | 清华大学 | Multi-scale tissue engineering composite scaffold and preparation device and preparation method thereof |
| CN113559329A (en) * | 2021-09-03 | 2021-10-29 | 北京大学口腔医学院 | Periodontal-imitated ordered double-layer structure stent material and preparation method and application thereof |
| CN115845139A (en) * | 2022-12-08 | 2023-03-28 | 海南宏正生物科技有限公司 | Artificial skin for promoting tissue repair and regeneration and preparation method thereof |
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| US6294187B1 (en) * | 1999-02-23 | 2001-09-25 | Osteotech, Inc. | Load-bearing osteoimplant, method for its manufacture and method of repairing bone using same |
| US8226715B2 (en) * | 2003-06-30 | 2012-07-24 | Depuy Mitek, Inc. | Scaffold for connective tissue repair |
| CN100404081C (en) * | 2006-06-09 | 2008-07-23 | 浙江大学 | Netted tissue-engineering stand |
| CN101209357A (en) * | 2007-12-21 | 2008-07-02 | 南京大学医学院附属鼓楼医院 | Method for preparing medical knee joint cruciate ligaments |
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