CN104597173A - Organophosphorus pesticide residue determination method - Google Patents
Organophosphorus pesticide residue determination method Download PDFInfo
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- CN104597173A CN104597173A CN201310534269.3A CN201310534269A CN104597173A CN 104597173 A CN104597173 A CN 104597173A CN 201310534269 A CN201310534269 A CN 201310534269A CN 104597173 A CN104597173 A CN 104597173A
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Abstract
An organophosphorus pesticide residue determination method comprises the following steps: screening main influence factors through gas chromatography by using quadrature test with the sample introduction amount of 1[mu]L, optimizing screened superior conditions, carrying out peak area external standard method quantification, diluting an acetone pesticide standard substance to the mass concentrations of 0.1mg/L, 0.2mg/L, 0.5mg/L, 0.8mg/L, 1.2mg/L and 2.0mg/L respectively, carrying out parallel determination on each of the above obtained work solutions five times, drawing a standard curve with the concentration as abscissa and the peak area as ordinate, and determining. The organophosphorus pesticide residue determination method is fast, sensitive, accurate and simple, and meets pesticide residue analysis prescriptions and requirements.
Description
Technical field
The present invention relates to Analytical Techniques of Pesticide Residues field, particularly Residues of Pesticides of Organic Phosphorus.
Background technology
Organophosphorus pesticide is efficient as a class, the pesticide of wide spectrum is just being widely used in the anti-evil of agricultural and the desinsection of family, storage etc., but the environmental hazard produced after a large amount of use is also day by day serious.Residues of pesticides act on human body accumulation and the teratogenesis caused, carcinogenic, mutagenesis harm by food chain, and as the acute and chronic poisoning of agricultural chemicals, colony's poisoning that particularly fruit-vegetable food causes after polluting occurs repeatedly.At present, generally believe that the organophosphorus pesticide of use is non-persistent agricultural chemicals both at home and abroad, degradation half life is greatly about several thoughtful some months.Research shows, organophosphorus pesticide also exists longer residual life under certain environmental conditions, and can at animals and plants body accumulation.The agricultural byproducts that long-term edible residues of pesticides exceed standard, directly can have influence on the health of the major organs such as liver, kidney, injure the nervous system of human body, cause the generation of disease, even have influence on follow-on health.Separately have test to confirm, agricultural chemicals may destroy certain specific health internal organs, such as: liver, kidney, marrow etc., but also can change the activity of enzyme, and Long Term Contact agricultural chemicals also can cause people's teratogenesis, carcinogenic.According to relevant epidemiologic investigation display, the Cancer Mortality risen year by year is relevant with the residues of pesticides in vegetables.The slight poisoning manifestations of organophosphorus pesticide is the symptom such as apocleisis, dizziness and headache, weakness, anxiety, uncomfortable in chest, myosis, moderate intoxication symptom for feeling sick, shedding tears, the phenomenon such as cramp, diarrhoea, perspiration, blood pressure be low, serious toxicity symptom is expiratory dyspnea, pulmonary edema, heartbeat are slack-off, even can cause the toxic reactions such as acute pancreatitis, and some organophosphorus pesticide also can cause DPN.The agricultural chemicals of contact high concentration there will be headache, weakness, feels sick, tics, the symptom such as similar influenza and malaria such as respiratory distress, even death, about has 20,000 people to die from pesticide poisoning every year.Poison that honeybee is poisoning also can cause abnormal gait with poison, poisoning advanced symptoms is better than the later stage, and showing in turn-club test poisoning early stage can be poorer.Acute organophosphorus pesticide poisoning there will be end stagerenaldisease, depression and anxiety, peripheral neuritis, hepatic renal dysfunction etc.For this reason, countries in the world are paid much attention to this problem, drop into a large amount of funds, man power and material, strengthen development and the application of nuisanceless green agricultural chemicals on the one hand; On the other hand, formulate more and more strict Pesticide Residue standard to prevent medicine and enter from mouth.
Summary of the invention
Of the present inventionly provide Pesticide-Polluted Soil detection method on probation, Residues of Pesticides of Organic Phosphorus, it is characterized in that, adopt gas chromatography, orthogonal test is utilized to screen major influence factors, to several groups that filter out preferably condition be optimized, wherein sample size is 1 μ L, peak area quantified by external standard method, the drafting acetone standard sample of pesticide of typical curve dilutes, mass concentration is respectively 0.1mg/L, 0.2mg/L, 0.5mg/L, 0.8mg/L, 1.2mg/L, 2.0mg/L, every part of working fluid replicate determination 5 times, take concentration as horizontal ordinate, peak area is ordinate drawing standard curve, gas flow is hydrogen 75mL/min, air 100mL/min, make-up gas 15mL/min, total carrier gas flux 60.0mL/min, injector temperature 200 DEG C.
The organophosphorus pesticide of described mensuration is DDVP, chlopyrifos, parathion, parathion-methyl, Rogor, omethoate, acephatemet or malathion.
Continuous 5 sample introductions of described method, the relative standard deviation of retention time is 0.01% ~ 0.12%, and the relative standard deviation of peak area is 1.8% ~ 4.5%.
Residues of Pesticides of Organic Phosphorus of the present invention is quick, sensitive, accurate, easy, meets farming residual analysis regulation and requirement.
Embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.
Embodiment 1
Residues of Pesticides of Organic Phosphorus, it is characterized in that, adopt gas chromatography, orthogonal test is utilized to screen major influence factors, to several groups that filter out preferably condition be optimized, wherein sample size is 1 μ L, peak area quantified by external standard method, the drafting acetone standard sample of pesticide of typical curve dilutes, mass concentration is respectively 0.1mg/L, 0.2mg/L, 0.5mg/L, 0.8mg/L, 1.2mg/L, 2.0mg/L, every part of working fluid replicate determination 5 times, take concentration as horizontal ordinate, peak area is ordinate drawing standard curve, gas flow is hydrogen 75mL/min, air 100mL/min, make-up gas 15mL/min, total carrier gas flux 60.0mL/min, injector temperature 200 DEG C.Described organophosphorus pesticide is DDVP, chlopyrifos, parathion, parathion-methyl, Rogor, omethoate, acephatemet or malathion.Continuous 5 sample introductions of described method, the relative standard deviation of retention time is 0.01% ~ 0.12%, and the relative standard deviation of peak area is 1.8% ~ 4.5%.
Claims (3)
1. Residues of Pesticides of Organic Phosphorus, it is characterized in that, adopt gas chromatography, orthogonal test is utilized to screen major influence factors, to several groups that filter out preferably condition be optimized, wherein sample size is 1 μ L, peak area quantified by external standard method, the drafting acetone standard sample of pesticide of typical curve dilutes, mass concentration is respectively 0.1mg/L, 0.2mg/L, 0.5mg/L, 0.8mg/L, 1.2mg/L, 2.0mg/L, every part of working fluid replicate determination 5 times, take concentration as horizontal ordinate, peak area is ordinate drawing standard curve, gas flow is hydrogen 75mL/min, air 100mL/min, make-up gas 15mL/min, total carrier gas flux 60.0mL/min, injector temperature 200 DEG C.
2. Residues of Pesticides of Organic Phosphorus according to claim 1, is characterized in that the organophosphorus pesticide of described mensuration is DDVP, chlopyrifos, parathion, parathion-methyl, Rogor, omethoate, acephatemet or malathion.
3. Residues of Pesticides of Organic Phosphorus according to claim 1, is characterized in that, continuous 5 sample introductions of described method, the relative standard deviation of retention time is 0.01% ~ 0.12%, and the relative standard deviation of peak area is 1.8% ~ 4.5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310534269.3A CN104597173A (en) | 2013-10-31 | 2013-10-31 | Organophosphorus pesticide residue determination method |
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| CN201310534269.3A CN104597173A (en) | 2013-10-31 | 2013-10-31 | Organophosphorus pesticide residue determination method |
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| CN104597173A true CN104597173A (en) | 2015-05-06 |
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| CN201310534269.3A Pending CN104597173A (en) | 2013-10-31 | 2013-10-31 | Organophosphorus pesticide residue determination method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107831257A (en) * | 2017-10-27 | 2018-03-23 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of organophosphorus residue quantity measuring method |
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2013
- 2013-10-31 CN CN201310534269.3A patent/CN104597173A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107831257A (en) * | 2017-10-27 | 2018-03-23 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of organophosphorus residue quantity measuring method |
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Application publication date: 20150506 |