CN104593473A - Method for detecting bacterial microbes in soil - Google Patents
Method for detecting bacterial microbes in soil Download PDFInfo
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- CN104593473A CN104593473A CN201310538524.1A CN201310538524A CN104593473A CN 104593473 A CN104593473 A CN 104593473A CN 201310538524 A CN201310538524 A CN 201310538524A CN 104593473 A CN104593473 A CN 104593473A
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Abstract
A method for detecting bacterial microbes in soil is characterized in that a sample is inoculated on an agar plate in an Archimedes spiral form through a screw plate making machine, the sample conveying amount reduces with the movement of a liquid conveying tube from the center of the plate to the edge of the plate, the sample is cultured, the plate is put on a counting grid, the grid is divided into sections with known areas, a bacterial colony is counted in the sections, a tiny amount of a radiocarbon marker is introduced to molecules of carbohydrate or salt substrate according to a principle that bacteria metabolize carbohydrates and carbon dioxide in the process of growth and reproduction, the substrate is used and releases radiocarbon when the bacteria grow, and an automatic radiation determinator is adopted to measure the content of the radiocarbon marker in order to determine the amount of the bacteria. The method for detecting bacterial microbes in soil allows a good result to be obtained by analyzing the content of bacterial components in the soil sample, is simple to operate, and provides a powerful tool for the detection of microbes in the soil.
Description
Technical field
The present invention relates to Soil K+adsorption technical field, particularly bacterial micro-organism detection method in soil.
Background technology
Microbial Biomineralization research shows, microorganism is in the formation of the evolution of the earth, Mineral resources, origin of life and environment for human survival purification now, and the aspect such as fixing as the fixing of carbonic acid gas and heavy metal plays important and irreplaceable effect.Under certain environment and nutritional condition stimulate; in soil, some microorganisms can separate out multi mineral crystallization significantly fast by metabolism or Degradation; as carbonate, phosphoric acid salt, oxide compound, sulfide, siliceous sinter and extracellular polymeric etc., and this may be slowly process in physical environment.Microorganism is all tiny organisms that are invisible or that do not see, individual small, structure is simple, usually amplifies about 1000 times with opticmicroscope and electron microscope and just can see, but also some microorganism is that naked eyes can be seen, as the mushroom, glossy ganoderma etc. that belong to fungi.Microorganism is in different poses and with different expressions, they in order to oneself existence with procreation also existing in oneself distinctive mode.The kind of Soil Microorganism is more, has bacterium, fungi, actinomycetes, algae and protozoon etc.Major part soil microorganisms is useful to crop growth, and they all have great effect to the formation growth of soil, material cycle and Fertility etc., and first, soil microorganisms can form Soil structure.Soil is not the simple combination of simple soil particle and chemical fertilizer, as the active group composition of soil, soil microorganisms is in the life process of oneself, by the oxygen of Metabolic activity and the exchange of carbonic acid gas, and the organic acid etc. of secretion contributes to soil particle and forms large crumb structure, the final soil formed truly.The fauna composition of soil microorganisms, biomass and vital movement thereof have substantial connection to the formation of soil and growth.The most significant effect of soil microorganisms decomposes organic matter exactly, and the undesirable root of such as crop loses leaf and organic fertilizer etc. in being manured into soil, only has the effect through soil microorganisms, could rot to decompose, discharge nutritive element, for crop utilization, and form soil ulmin, improve structure and the tilth of soil.Soil microorganisms can also decompose mineral substance, and the meta-bolites of soil microorganisms can promote the dissolving of insoluble material in soil.Such as phosphorus bacteria can decomposite the phosphorus in Rock Phosphate (72Min BPL), and B. mucilaginocus can decomposite the potassium in potassium ore, utilizes, increase soil fertility in order to Crop.In addition, the decomposition of urea utilizes and also be unable to do without soil microorganisms.Mineral fertilizer in soil is processed into the form that crop can absorb by microorganism.Soil microorganisms also has nitrogen fixation, 4/5 of nitrogen duty gas composition, but plant can not directly utilize, and the nitrogen in air can be converted into by its nitrogen fixation the stationary state nitride that plant can utilize by certain micro-organisms.From resources of soil microorganisms separation screening, detect microorganism, be should the important process of strengthening research.
Summary of the invention
The method that bacterial micro-organism in efficient detection soil is provided of the present invention, technical scheme is as follows:
Bacterial micro-organism detection method in soil, it is characterized in that, describedly by spiral laminator, sample is seeded on agar plate with the form of spiral of Archimedes, defeated sample amount reduces from the extrorse movement of plate center along with tubing, after cultivating, flat board is placed on counting lattice, it is interval that these lattice are divided into some known area, colony number is namely in this interval inside counting, the principle of carbonic acid gas is produced according to bacterium metabolize carbohydrates in growth and breeding process, the radiocarbon C14 of trace mark is introduced in carbohydrate or salt substrate molecule, during bacterial growth, substrate is utilized and discharges containing radiocarbon C14, the content being measured radiocarbon C14 mark by Automated radio determinator determines amount of bacteria.
Described method is for detecting vinelandii, nitrobacteria and saprophytic bacteria.
Described sample preparation method, for the earth that fetches earth is as experiment soil, spends ionized water and makes suspension liquid, and vibration mixing 20min, deposits for 2 DEG C.
Described every 100g soil adds 1000ml water.
Bacterial micro-organism detection method in soil of the present invention, analyze the bacterial component content in pedotheque, obtain good result, method is easy and simple to handle, and the detection for Soil Microorganism provides strong instrument.
Embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.
Embodiment 1
Bacterial micro-organism detection method in soil, it is characterized in that, describedly by spiral laminator, sample (every 100g soil adds 1000ml water) is seeded on agar plate with the form of spiral of Archimedes, defeated sample amount reduces from the extrorse movement of plate center along with tubing, after cultivating, flat board is placed on counting lattice, it is interval that these lattice are divided into some known area, colony number is namely in this interval inside counting, the principle of carbonic acid gas is produced according to bacterium metabolize carbohydrates in growth and breeding process, the radiocarbon C14 of trace mark is introduced in carbohydrate or salt substrate molecule, during bacterial growth, substrate is utilized and discharges containing radiocarbon C14, the content being measured radiocarbon C14 mark by Automated radio determinator determines amount of bacteria, method is for detecting vinelandii, nitrobacteria and saprophytic bacteria, sample preparation method is for the earth that fetches earth is as experiment soil, spend ionized water and make suspension liquid, vibration mixing 20min, deposit for 2 DEG C.
Claims (4)
1. bacterial micro-organism detection method in soil, it is characterized in that, describedly by spiral laminator, sample is seeded on agar plate with the form of spiral of Archimedes, defeated sample amount reduces from the extrorse movement of plate center along with tubing, after cultivating, flat board is placed on counting lattice, it is interval that these lattice are divided into some known area, colony number is namely in this interval inside counting, the principle of carbonic acid gas is produced according to bacterium metabolize carbohydrates in growth and breeding process, the radiocarbon C14 of trace mark is introduced in carbohydrate or salt substrate molecule, during bacterial growth, substrate is utilized and discharges containing radiocarbon C14, the content being measured radiocarbon C14 mark by Automated radio determinator determines amount of bacteria.
2. bacterial micro-organism detection method in soil according to claim 1, is characterized in that, described method is for detecting vinelandii, nitrobacteria and saprophytic bacteria.
3. bacterial micro-organism detection method in soil according to claim 1, it is characterized in that described sample preparation method is for the earth that fetches earth is as experiment soil, spend ionized water and make suspension liquid, vibration mixing 20min, deposits for 2 DEG C.
4. bacterial micro-organism detection method in soil according to claim 3, is characterized in that described every 100g soil adds 1000ml water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310538524.1A CN104593473A (en) | 2013-10-31 | 2013-10-31 | Method for detecting bacterial microbes in soil |
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| CN201310538524.1A CN104593473A (en) | 2013-10-31 | 2013-10-31 | Method for detecting bacterial microbes in soil |
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| CN104593473A true CN104593473A (en) | 2015-05-06 |
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| CN201310538524.1A Pending CN104593473A (en) | 2013-10-31 | 2013-10-31 | Method for detecting bacterial microbes in soil |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105695556A (en) * | 2016-02-18 | 2016-06-22 | 深圳市依波特科技有限公司 | Screw inoculation method |
| CN115445274A (en) * | 2022-07-19 | 2022-12-09 | 中国水产科学研究院南海水产研究所 | Device and method for separating and collecting organic carbon discharged by metabolism of zooplankton |
-
2013
- 2013-10-31 CN CN201310538524.1A patent/CN104593473A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105695556A (en) * | 2016-02-18 | 2016-06-22 | 深圳市依波特科技有限公司 | Screw inoculation method |
| CN115445274A (en) * | 2022-07-19 | 2022-12-09 | 中国水产科学研究院南海水产研究所 | Device and method for separating and collecting organic carbon discharged by metabolism of zooplankton |
| CN115445274B (en) * | 2022-07-19 | 2024-02-02 | 中国水产科学研究院南海水产研究所 | Device and method for separating and collecting zooplankton metabolism emission organic carbon |
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Application publication date: 20150506 |