CN104593147A - Method for degumming magnetic immobilized phospholipase A1 in liquid-solid two-phase fluidized bed - Google Patents
Method for degumming magnetic immobilized phospholipase A1 in liquid-solid two-phase fluidized bed Download PDFInfo
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- CN104593147A CN104593147A CN201510028348.6A CN201510028348A CN104593147A CN 104593147 A CN104593147 A CN 104593147A CN 201510028348 A CN201510028348 A CN 201510028348A CN 104593147 A CN104593147 A CN 104593147A
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- liquid
- solid
- magnetic immobilized
- immobilized phospholipase
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Classifications
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
Abstract
The invention provides a method for degumming magnetic immobilized phospholipase A1 (PLA1) in a liquid-solid two-phase fluidized bed. Physical refining can be realized by adopting phospholipase A1 to degum, and a nutritive value of the phospholipid also can be kept to the greatest extent. Free enzyme is not prone to separate and recycle in a reaction system, high in cost, difficult to recycle or continuously use, and therefore, the invention discloses the method for degumming magnetic immobilized phospholipase A1 in the liquid-solid two-phase fluidized bed. The magnetic immobilized phospholipase has the effects of being repeatedly used and easily recycled, so that the activity and the stability of the enzyme in the reaction system are improved. The fluidized bed can realize continuous production, and thus, the method realizes continuous dynamic degumming, is free of stirring in a reaction process, can continuously separate the magnetic enzyme from a liquid-state mobile phone, and solves a problem that the enzyme and the degummed oil are difficult to separate.
Description
Technical field
The present invention relates to one magnetic immobilized phospholipase A in liquid-solid two-phase fluidisation bed
1the method of coming unstuck.
Background technology
In fats and oils processing industry, fat degumming is one of focus of paying close attention to of people always, and coming unstuck is exactly the peptized impurities removed in oil, and main component is phosphatide.Phosphatide is mainly divided into hydrated phospholipid and non-hydratable phospholipid, and hydrated phospholipid is by the removing of traditional hydration degum method, but non-hydratable phospholipid has significant hydrophobicity, is difficult to remove.And traditional Degumming method mainly contains hydration degum, absorption is come unstuck, acid degumming, enzymatic degumming etc. are used for processing crude oil, reduce the method for peptized impurities content in crude oil.As a rule, often kind of method has himself feature and use range, according to the factor such as the requirement of different oil refining process, production unit input, environment, chooses the Degumming method of the most applicable requirement.In these degumming technologys, enzymatic degumming is subject to increasing attention with its good economic environmental-protecting performance, and this technology has huge using value in the economic benefit improving oil prodution industry and environmental benefit.
Traditional enzymatic degumming is the fatty acid chain of hydrolytic phosphatide and generates hemolytic phosphatide, can be removed by the method for aquation.Phospholipid hydrolase comes unstuck and to compare with traditional Degumming method, greatly can save the consumption of chemical substance, produce waste water hardly, in environmental protection, economy, quality etc., have potential advantage.And phospholipase A
1(PL A
1) can the enzyme of specific for hydrolysis natural phospholipid Sn-1 position acyl group, generate lysophospholipid, retain the unsaturated fatty acids of phosphatide Sn-2 position; Therefore, phospholipase A is adopted
1come unstuck, can not only physical refining be realized, also can retain the nutritive value of phosphatide itself to greatest extent.
Summary of the invention
The present invention be directed to resolvase not only not easily Separation and Recovery in reaction system, and enzyme produce general more difficult, and with high costs, to be difficult to repeatedly or the problem such as uses continuously, and the magnetic immobilized phospholipase A in liquid-solid two-phase fluidisation bed of proposition
1carry out the method for coming unstuck.Magnetic immobilized phospholipase A in liquid-solid two-phase fluidisation bed
1the method of carrying out coming unstuck is realized by following steps: one, magnetic immobilized phospholipase A
1preparation; Two, magnetic immobilized phospholipase A in liquid-solid two-phase fluidisation bed
1come unstuck.
Magnetic immobilized phospholipase A in liquid-solid two-phase fluidisation bed
1in coming unstuck, magnetic immobilized enzyme has the effect of reusing and being easy to reclaim, and improves the activity of enzyme in reaction system and stability.Because fluidized-bed can realize continuous prodution, therefore present invention achieves and dynamically come unstuck continuously, reaction process is without the need to stirring, and magnetic enzyme and liquid moving phase can be continuously separated, and solves the problem that enzyme and degummed oil are difficult to be separated.
Embodiment
Embodiment one: one magnetic immobilized phospholipase A in liquid-solid two-phase fluidisation bed
1the method of coming unstuck is realized by following steps: one, magnetic immobilized phospholipase A
1preparation: first by the magnetic Fe of lg
3o
4/ SiOx-g-P (GMA) composite particles is placed at the phosphoric acid buffer (0.1M of 50mL, pH 7.0) middle immersion 24h, carrier after above-mentioned immersion is joined a certain amount of phosphoric acid buffer (0.1M containing the Phospholipid hydrolase of 0.2% after Magneto separate, pH 7. 0) in, stirring reaction for some time at 45 DEG C simultaneously, last Magneto separate also uses phosphoric acid buffer (0.1M, pH 7.0) being fixed of washing enzyme, the immobilized enzyme of gained is placed in refrigerator and preserves at 4 DEG C, for subsequent use; Two, magnetic immobilized phospholipase A in liquid-solid two-phase fluidisation bed
1come unstuck: crude oil of soybean is passed in magnetofluidized bed bioreactor, at magnetic immobilized phospholipase A
1addition 0.1g/kg, amount of water 2.0ml, pH 6.7 and add a certain amount of borate buffer solution condition under carry out reaction of coming unstuck, and regulate magneticstrength and liquid phase flow rate, after 55 DEG C of reaction for some time, sampling and measuring degummed oil phosphorus content.
Embodiment two: it is 0.8 ~ 1.2mL/100g that the difference of present embodiment and embodiment one to be in step 2 at boric acid addition, magneticstrength is 0.018 ~ 0.026T, liquid phase flow rate is 0.0020 ~ 0.0030m/s, temperature of reaction 40 ~ 65 DEG C, under the condition of reaction times 2.8 ~ 3.8h, carry out magnetic immobilized phospholipase A1 in liquid-solid two-phase fluidisation bed to come unstuck, the degummed oil phosphorus content obtained with this understanding is at below 13mg/kg.Other step is identical with embodiment one.
Claims (2)
1. a magnetic immobilized phospholipase A in liquid-solid two-phase fluidisation bed
1the method of coming unstuck, is characterized in that magnetic immobilized phospholipase A in liquid-solid two-phase fluidisation bed
1the method of coming unstuck is realized by following steps: one, magnetic immobilized phospholipase A
1preparation: first by the magnetic Fe of lg
3o
4/ SiOx-g-P (GMA) composite particles is placed at the phosphoric acid buffer (0.1M of 50mL, pH 7.0) middle immersion 24h, carrier after above-mentioned immersion is joined a certain amount of phosphoric acid buffer (0.1M containing the Phospholipid hydrolase of 0.2% after Magneto separate, pH 7. 0) in, stirring reaction for some time at 45 DEG C simultaneously, last Magneto separate also uses phosphoric acid buffer (0.1M, pH 7.0) being fixed of washing enzyme, the immobilized enzyme of gained is placed in refrigerator and preserves at 4 DEG C, for subsequent use; Two, magnetic immobilized phospholipase A in liquid-solid two-phase fluidisation bed
1come unstuck: crude oil of soybean is passed in magnetofluidized bed bioreactor, at magnetic immobilized phospholipase A
1addition 0.1g/kg, amount of water 2.0ml, pH 6.7 and add a certain amount of borate buffer solution condition under carry out reaction of coming unstuck, and regulate magneticstrength and liquid phase flow rate, after 55 DEG C of reaction for some time, sampling and measuring degummed oil phosphorus content.
2. according to claim 1 in liquid-solid two-phase fluidisation bed magnetic immobilized phospholipase A
1the method of coming unstuck, to it is characterized in that in step 2 at boric acid addition being 0.8 ~ 1.2mL/100g, magneticstrength is 0.018 ~ 0.026T, liquid phase flow rate is 0.0020 ~ 0.0030m/s, temperature of reaction 40 ~ 65 DEG C, under the condition of reaction times 2.8 ~ 3.8h, carry out magnetic immobilized phospholipase A in liquid-solid two-phase fluidisation bed
1come unstuck, the degummed oil phosphorus content obtained with this understanding is at below 13mg/kg.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510028348.6A CN104593147A (en) | 2015-01-21 | 2015-01-21 | Method for degumming magnetic immobilized phospholipase A1 in liquid-solid two-phase fluidized bed |
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|---|---|---|---|
| CN201510028348.6A CN104593147A (en) | 2015-01-21 | 2015-01-21 | Method for degumming magnetic immobilized phospholipase A1 in liquid-solid two-phase fluidized bed |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105602718A (en) * | 2016-03-10 | 2016-05-25 | 东北农业大学 | Method for conducting degumming on crude soybean oil through magnetic immobilized phospholipase C and magnetic immobilized phospholipase A1 |
| WO2017020143A1 (en) * | 2015-08-03 | 2017-02-09 | 于殿宇 | Method of biosynthesizing functional lipid using nano-magnetically immobilized enzyme for continuous targeted catalysis of phospholipid in soybean oil |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559378A (en) * | 2010-11-10 | 2012-07-11 | 东北农业大学 | Method for refining raw oil by using magnetic immobilized phospholipase A1 |
-
2015
- 2015-01-21 CN CN201510028348.6A patent/CN104593147A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559378A (en) * | 2010-11-10 | 2012-07-11 | 东北农业大学 | Method for refining raw oil by using magnetic immobilized phospholipase A1 |
Non-Patent Citations (2)
| Title |
|---|
| 张世涛等: "磁场稳定流化床反应器水解牛乳中乳糖的研究", 《食品与机械》 * |
| 张康逸: "水相体系酶法制备甘油磷脂酰胆碱及酰基转移机理研究", 《中国博士学位论文全文数据库（电子期刊）工程科技Ⅰ辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017020143A1 (en) * | 2015-08-03 | 2017-02-09 | 于殿宇 | Method of biosynthesizing functional lipid using nano-magnetically immobilized enzyme for continuous targeted catalysis of phospholipid in soybean oil |
| CN107532110A (en) * | 2015-08-03 | 2018-01-02 | 于殿宇 | The method of phosphatide biological synthetic functional lipid in nano-magnetic immobilised enzymes continuously-directional catalysis crude oil of soybean |
| CN105602718A (en) * | 2016-03-10 | 2016-05-25 | 东北农业大学 | Method for conducting degumming on crude soybean oil through magnetic immobilized phospholipase C and magnetic immobilized phospholipase A1 |
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Application publication date: 20150506 |