CN104582508A - A method for the preparation of a serum protein concentrate - Google Patents

A method for the preparation of a serum protein concentrate Download PDF

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Publication number
CN104582508A
CN104582508A CN201380042937.9A CN201380042937A CN104582508A CN 104582508 A CN104582508 A CN 104582508A CN 201380042937 A CN201380042937 A CN 201380042937A CN 104582508 A CN104582508 A CN 104582508A
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protein
whey
solution
grams per
concentrate
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CN104582508B (en
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克里斯蒂安·莱比勒
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LB LYOPHARM Srl
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LB LYOPHARM Srl
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
    • A23J1/205—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey from whey, e.g. lactalbumine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C21/00—Whey; Whey preparations
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/14—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment
    • A23C9/142—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment by dialysis, reverse osmosis or ultrafiltration
    • A23C9/1425—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment by dialysis, reverse osmosis or ultrafiltration by ultrafiltration, microfiltration or diafiltration of whey, e.g. treatment of the UF permeate
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/66—Proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/19—Dairy proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
    • A61K8/986—Milk; Derivatives thereof, e.g. butter
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/04—Animal proteins
    • A23J3/08—Dairy proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/10—General cosmetic use

Abstract

The present invention relates to a method for the preparation of a concentrate of serum proteins with reduced lactose content from a sample of lactoserum, wherein said concentrate is characterized in that it comprises a concentration of 0.5 grams of lactose per kilogram of protein, the concentrate obtainable from said process and food products containing said concentrate.

Description

Prepare the method for serum proteins concentrate
Technical field
The present invention relates to and a kind ofly prepare the method for protein concentrates and comprise the product of this concentrate with whey sample, wherein the feature of this concentrate is that it has the concentration of every kilogram of protein≤0.5 gram lactose, and this concentrate can obtain from the method.
Background technology
Whey (whey) (or " whey (lactoserum) ") represents newborn liquid part and except protein, mineral salt, trace lipid and lactose degradation product (lactic acid, glucose, galactolipin), also comprises the lactose of high percentage composition (between 50% to 75%).The protein content of whey is in about 8% to 14% scope; Especially, the existence of protein mixture is possible, and the main protein in protein is albumin, beta lactoglobulin, ALA, seralbumin, lactoferrin, immunoglobulin (Ig); Aminess (leucine, isoleucine, valine, threonine, tryptophan, lysine, phenylalanine etc.).
Various research demonstrates whey protein and how to have beneficial effect to health.Especially, Wong etc. report: owing to there is immunoglobulin class in whey protein, and whey protein is taken in and enhanced immunologic function (Wong & Watson 1995.Immunomodulatory effects of dietary whey proteins in mice.J Dairy Res.62 (2): 359-68).In addition, also propose whey protein and take in how can have prevention effect (Hakkak etc., Dietary wheyprotein protects against azoxymethane-induced colon tumors in male rats.Cancer Epidemiol.Biomarkers Prev.10 (5): 555 – 8.PMID 11352868) to antagonism cancer development.
From medical science viewpoint, special interests not only points to whey immunoglobulin class, also points to lactoferrin, and the beneficial effect (such as improve immunity, antibacterial, antiviral, active anticancer) of lactoferrin to health is described.
Whey protein is also famous to the beneficial effect of skin with it.
Even if as mentioned above, a series of beneficial effect is associated with whey protein, and the feature of the whey that the absorption of whey protein or use are present in wherein by whey protein is usually that the fact of high-load lactose limits.Especially, in world population, be general to the not resistance to disease of this molecule; This is mainly because cannot metabolising lactose owing to lacking lactase experimenter.The generation of this not resistance to disease significantly changes because of country, is the percentage of about 22%, reaches about 70% in the population of southern Europe in the adult population of the U.S..
The symptom that the most general lactose intolerance is relevant be substantially stomach abnormal (such as stomachache and abdominal cramps, intestines level feel swelling and tighten, increase of wriggling, aerogastria, bulging, diarrhoea (diarrhoic bowel movement) etc.).
The treatment of the most excellent lactose intolerance is the scheme of going on a diet, and wherein the contribution of rich lactase-containing comestibles (therefore comprising whey) reduces.But this treatment is unsuitable for that not resistance to disease experimenter is the children experimenter of the basic suitable distribution to ensure whey protein for such as wherein whey is taken in especially, underfed experimenter, have the situation of the experimenter etc. of immune deficiency.
Therefore, the situation sensation of known art need to limit allow purifying from the serum proteins of whey mixture, the content of the lactose existed in whey is minimized and therefore ensures the technology of maintenance of composition of typical protein, amino acid etc. in whey.
Summary of the invention
The present invention relates to a kind of method preparing protein concentrates with whey (lactoserum) (also referred to as whey (whey)).Especially, the method comprises sequence of operations step, and the final result of this series of operative steps obtains protein (or also referred to as " serum proteins ") concentrate with particularly advantageous characteristics from whey.
In fact, the method described in the application allows the content reducing the lactose existed in whey, and maintains the content of the lactose in serum proteins, therefore, in other words, does not have the peculiar composition changing or change the lactose in protein, amino acid, mineral salt etc.
Especially, the feature of the obtainable serum proteins concentrate of the method described by the application is that it has the concentration of every kilogram of protein≤0.5 gram lactose.In addition, there is from the protein be separated according to whey described below the purity of at least 96%.
Therefore, the method of the object of this description can be advantageously used in prepares serum proteins concentrate with whey, and this serum proteins concentrate is intended to for such as having lactose intolerance or hypersensitive experimenter and/or needing the experimenter of major contribution of serum proteins.
In addition, author of the present invention also observes, and it is not containing bad smell and taste, the serum proteins concentrate typically replacing whey protein that the method that the application describes creates feature.The easier purposes (by example but be not limited to) that in final analysis, this another advantage determines such as this concentrate in subsequent stage (such as comprising the preparation of its product) can be food, cosmetics, biological technology products etc.
Therefore, the first object of the present invention is a kind of method with the preparation of whey sample with the serum proteins concentrate of the lactose content of reduction, and the method comprises the following steps:
A) this whey sample concentrated, therefore obtains the protein concentration between about 150 grams per liters and 300 grams per liters;
B) this whey concentrate is experienced at least one diafiltration steps;
C) dilute the protein concentrates of this diafiltration, therefore obtain the solution of protein concentration within the scope of about 50 grams per liter to 90 grams per liters;
D) in this solution, add the fumed silica of often liter of solution about 5 to 10 grams;
E) ethanol (95%, volume/volume) of every kilogram of protein about 70 to 300ml is added this solution;
F) by step e) the pH acidifying of solution that obtains is the value of about 4.5 to 5;
G) solution of acidifying is heated to temperature between about 55 DEG C and 70 DEG C at least 20 minutes, wherein this temperature reached in about 30 minutes;
H) solution is cooled to the temperature between about 10 DEG C and 20 DEG C;
I) separation of the protein solution cooled is carried out;
L) pH of separation solution is brought to the value of about 5.8 to 6.8;
M) by step l) solution that obtains experiences the process of at least one micro porous filtration, therefore obtains the protein concentrates of protein concentration between about 100 grams per liters and 300 grams per liters;
N) protein concentrates is experienced at least one diafiltration steps;
O) dry and/or this protein concentrates of freeze drying,
Concentration wherein from this serum proteins concentrate of whey is every kilogram of protein≤0.5 gram lactose.
Second object of this description is protein (or serum proteins) concentrate from whey, and every kilogram of protein comprises≤0.5 gram of lactose.
The other object of the description of the application is comprise the food of above whey protein concentrate, cosmetics or biological technology products.
Detailed description of the invention
The present invention relates to a kind of method with the preparation of whey sample with the serum proteins concentrate of the lactose content of reduction.In other words, the method that the application describes allows the protein mixture comprised in sepg whey, reduces or eliminates the lactose existed in the original samples of whey.
In this description, term " whey (lactoserum) " or " whey (whey) " refer to from the liquid part that obtains of rich milk, skimmed milk or half skimmed milk after rennet is separated.
Especially, the method for the object of this description comprises following operating procedure:
A) this whey sample concentrated, therefore obtains the protein concentration between about 150 grams per liters and 300 grams per liters;
B) this whey concentrate is experienced at least one diafiltration steps;
C) dilute the protein concentrates of this diafiltration, therefore obtain the solution of protein concentration within the scope of about 50 grams per liter to 90 grams per liters;
D) in this solution, add the fumed silica of often liter of solution about 5 to 10 grams;
E) ethanol (95%, volume/volume) of every kilogram of protein about 70 to 300ml is added this solution;
F) by step e) the pH acidifying of solution that obtains is the value of about 4.5 to 5.0;
G) solution of acidifying is heated to temperature between about 55 DEG C and 70 DEG C at least 20 minutes, wherein this temperature reached in about 30 minutes;
H) solution is cooled to the temperature between about 10 DEG C and 20 DEG C;
I) separation of the protein solution cooled is carried out;
L) pH of separation solution is brought to the value of about 5.8 to 6.8;
M) by step l) solution that obtains experiences the process of at least one micro porous filtration, therefore obtains the protein concentrates of protein concentration between about 100 grams per liters and 300 grams per liters;
N) protein concentrates is experienced at least one diafiltration steps;
O) dry and/or this protein concentrates of freeze drying,
Concentration wherein from this serum proteins concentrate of whey is every kilogram of protein≤0.5 gram lactose.
The preparation of the protein concentrates of the method described according to the application clearly maybe can comprise only sweet whey or whey sample that only yogurt is clear for raw material and carries out can comprise sweet whey and yogurt.The whey useful to object of the present invention can be any source; Therefore by means of only non-limiting example, this whey can obtain from human milk, cow's milk or sheep breast etc.
As implied above, the method comprises the step of condensed whey original samples, and this step known and any one technology being suitable for this object can be carried out according to those skilled in the art.In the preferred embodiment of the method, concentration step is undertaken by ultrafiltration.Such as, according on show step a), ultrafiltration retains (cut-off) for film between about 10000 dalton and 15000 dalton by use and carries out.Therefore, by illustrate, can use retain be 10000,11000,12000,13000,14000 and/or 15000 film.In order to obtain the object that protein concentration is the whey of at least 150 grams per liters, one or more ultrafiltration step can be needed and/or use one or more film described above.
Next stage (i.e. the step b of above method)) be to reduce the maximum possible amount according to lactose in above-mentioned whey concentrate.Whole elimination of lactose content or part reduce can such as be undertaken by least one diafiltration steps." diafiltration " generally refers to that the solution of micro solutes with molecule (being protein in this situation) is separated by the ultrafiltration by adding solvent continuously.Such as, according to the diafiltration of method of the present invention by making to spend mineral water, distilled water and/or infiltration water instead of saline solution carries out as solvent.By means of only example, every volume whey concentrate can use three volumes to remove mineral water.In one embodiment of the invention, the diafiltration steps of whey concentrate is at least 3 times and every volume whey concentrate uses three volumes to remove mineral water to carry out.
Then by due to above compared with starting whey sample feature be the whey of the diafiltration of the lactose content reduced by adding solvent dilution, therefore obtaining protein content is the solution of the whey of the diafiltration of about 50 grams per liter to 90 grams per liters.Especially, the protein content of this solution can be about 50 grams per liters, 51 grams per liters, 52 grams per liters, 53 grams per liters, 54 grams per liters, 55 grams per liters, 56 grams per liters, 57 grams per liters, 58 grams per liters, 59 grams per liters, 60 grams per liters, 61 grams per liters, 62 grams per liters, 63 grams per liters, 64 grams per liters, 65 grams per liters, 66 grams per liters, 67 grams per liters, 68 grams per liters, 69 grams per liters, 70 grams per liters, 71 grams per liters, 72 grams per liters, 73 grams per liters, 74 grams per liters, 75 grams per liters, 76 grams per liters, 77 grams per liters, 78 grams per liters, 79 grams per liters, 80 grams per liters, 81 grams per liters, 82 grams per liters, 83 grams per liters, 84 grams per liters, 85 grams per liters, 86 grams per liters, 87 grams per liters, 88 grams per liters, 89 grams per liters, 90 grams per liters.In one embodiment of the invention, the protein concentration of the solution of the whey of diafiltration is about 70 grams per liters.Such as, the dilution of the solution of the whey of diafiltration is carried out as solvent by making to spend mineral water.
The steps d of method that the application describes) comprise in the whey solution of dilution, to add the fumed silica of often liter of solution about 5 grams to 10 grams, such as 7 gram.
Fumed silica is the silica of the particular type be made up of the microscopic droplets of the amorphous silica being agglomerated into the tertiary granule with specified chemical physical property.Usually the fumed silica be present in powder form on market is to fix the lipoprotein, remaining fatty material and/or the macromolecular hydrophilic fumed silica that exist in given solution.At this moment, do not need to describe fumed silica in detail because its to be those skilled in the art know and widely used in various technical field.Be by means of only example and restriction the object of the invention, fumed silica can be type, such as 380 and/or 200.
After adding above silica, every kilogram of protein about 70 to 300ml ethanol (95%, volume/volume) is added solution.Such as, the amount of the ethanol added is that every kilogram of protein is about 120ml and even more; Such as, ethanol is edible rank.
Afterwards, the step f of the method described according to the application), such as, by use HCl, the pH of solution is brought to the value of about 4.5 to 5.0.Step f) at the end of pH value of solution can be 4.6,4.7,4.8,4.9,5.0 pH; Such as pH will have the value of 4.6.
Then the acid solution obtained like this is heated to temperature between 55 DEG C and 77 DEG C at least 20 minutes.Especially, this heating-up temperature can be 55 DEG C, 56 DEG C, 57 DEG C, 58 DEG C, 59 DEG C, 60 DEG C, 61 DEG C, 62 DEG C, 63 DEG C, 64 DEG C, 65 DEG C, 66 DEG C, 67 DEG C, 68 DEG C, 69 DEG C, 70 DEG C, such as 66 DEG C.For the purposes of the present invention, the heating (in fact comprise and temperature is elevated to associated temperature described above from the initial temperature of whey original samples) that the application describes should occur in the interval of at least 30 minutes.Especially, the inventor of this method observes, solution heating faster determines the loss of the productive rate of the serum proteins of purifying, and slower solution heating determines unsatisfied purifying of serum proteins, because uncorrelated protein (such as casein) is not eliminated.
Afterwards, between 10 DEG C and 20 DEG C, be therefore cooled to by the solution of heating is the lower temperature of 10 DEG C, 11 DEG C, 12 DEG C, 13 DEG C, 14 DEG C, 15 DEG C, 16 DEG C, 17 DEG C, 18 DEG C, 19 DEG C or 20 DEG C.
The solution of this cooling experience is separated to be separated the part comprising serum proteins.This step I of method that the application describes) can think according to those skilled in the art and be suitable for allowing any one separation/purification technology be separated with above solution by protein component to carry out.By means of only example, be separated by using Alfa laval separator or Westfalia separator to carry out continuously.
Then the pH comprising the separation solution of serum proteins is such as brought to the value between 5.8 to 6.8 by use NaOH.This step l) at the end of pH value of solution can be 5.8,5.9,6.0,6.1,6.2,6.3,6.4,6.5,6.6,6.7 or 6.8 pH; Such as, pH can have the value of 6.4.
Then, the step m according to the method for the object of this description), the solution of serum proteins is experienced the process of at least one micro porous filtration, therefore obtains the protein concentrates of protein concentration between 100 grams per liters and 300 grams per liters.In one embodiment of the invention, the micro porous filtration film retained as about 12 microns or 0.6 micron carries out.Especially, to retain be that the film of 12 microns carries out, next for using the one or more processes retaining the micro porous filtration of the film being 6 microns by using at least one first process of micro porous filtration.Such as, the micropore filtering film used in the method that the application describes is organic film and/or ceramic membrane.
Afterwards, the solution of the micro porous filtration of serum proteins is experienced at least one diafiltration steps, this diafiltration steps can with the step b of the method for this description) in the step that described carry out similarly.
Finally, by the whey protein solution of micro porous filtration and diafiltration, dry and/or freeze drying, obtains serum proteins concentrate with whey thus.
From the concentrate of the protein of whey or be characterised in that the concentration with every kilogram of protein≤0.5 gram lactose especially from the obtainable serum proteins concentrate of said method.In addition, the method creates serum proteins concentrate, and the purity of the protein wherein comprised in serum proteins concentrate is at least 96%.
In addition, be also not have according to the feature of the obtainable protein concentrates of content disclosed in the present application the bad smell and/or taste that whey protein typical case has.
As implied above, the chemical physical property of this concentrate makes it advantageous particularly under the condition (having the situation of not resistance to disease and/or hypersensitive experimenter to lactose as wherein its expection final use relates to) needing to make the content of the lactose wherein existed be reduced to minimum of a value or such as do not exist lactose.Already mentioned according to the application, the object of the application is also the product comprising above-mentioned protein concentrates.Especially, this product can be used for any field, such as, such as, when not limiting the application, and food, cosmetics, biological technical field.By means of only example, food can be for baby, sick experimenter, children, old man or is simply the food in accordance with the nutrition of the people of specific scheme of going on a diet, such as soup, Yoghourt, milk, fruit juice, dessert etc.At cosmetic field, on the contrary, the concentrate of object of the present invention can be included in moisturizer or usually can provide in the product of nutrition to Skin Cell.In addition, at biological technical field, serum proteins concentrate such as can be used as the element used in mammalian cell or the culture medium such as the preparation of the microorganism of recombinant protein.
The concentration of this product every kilogram protein is identical with the concentration of every kilogram of protein of the concentrate of the application's book.
According in the product of the application's book, serum proteins are the serum proteins comprised according to the concentrate of the application, and therefore product itself will have the lactose concn equaling every kilogram of whey protein≤0.5 gram lactose.
When comprising the product as milk or Yoghourt of concentrate of this description, in order to keep the concentration that there are not lactose and raising serum proteins, obtain the product with more larger protein concentration thus, go lactose milk to can be used as starting products, the concentrate of this description will be added in this starting products.
example
example 1:food is made to be rich in whey protein concentrate.
Comprise the food of serum proteins concentrate by following preparation.
Soup: to the soup part between 150ml and 250ml, the concentrate between 10g and 15g;
Milk: to the milk portion between 150ml and 250ml, the serum proteins between 5g and 10g;
Yoghourt: to the Yoghourt part between 100ml and 250ml, the concentrate between 5g and 10g;
Fruit juice: to the part between 100ml and 1000ml, the concentrate between 5g and 10g;
Dessert: to the dessert part between 100ml and 250ml, the concentrate between 5g and 10g.

Claims (17)

1. there is with the preparation of whey sample a method for the serum proteins concentrate of the lactose content of reduction, comprise the following steps:
A) concentrated described whey sample, therefore obtains the protein concentration between about 150 grams per liters and 300 grams per liters;
B) described whey concentrate is experienced at least one diafiltration steps;
C) dilute the described protein concentrates of diafiltration, therefore obtain the solution of protein concentration within the scope of about 50 grams per liter to 90 grams per liters;
D) in described solution, add the fumed silica of 5 grams to 10 grams, often liter of solution York;
E) the 95% volume/volume ethanol every kilogram of protein being about 70ml to 300ml adds described solution;
F) by step e) the pH acidifying of described solution that obtains is the value of about 4.5 to 5.0;
G) the described solution of acidifying is heated to temperature between about 55 DEG C and 70 DEG C at least 20 minutes, wherein said temperature reached in about 30 minutes;
H) described solution is cooled to the temperature between about 10 DEG C and 20 DEG C;
I) separation of the described protein solution cooled is carried out;
L) pH of the described solution be separated is brought to the value of about 5.8 to 6.8;
M) by step l) the described solution that obtains experiences the process of at least one micro porous filtration, therefore obtains the protein concentrates of protein concentration between about 100 grams per liters and 300 grams per liters;
N) described protein concentrates is experienced at least one diafiltration steps;
O) protein concentrates described in dry and/or freeze drying,
Concentration wherein from the described protein concentrates of whey is every kilogram of protein≤0.5 gram lactose.
2. method according to claim 1, wherein said whey sample comprises sweet whey and/or yogurt is clear.
3. the method according to aforementioned claim 1 or 2, wherein step a) and step n) described in be concentrated by ultrafiltration to carry out.
4. method according to claim 3, wherein said ultrafiltration is with retaining as the film between about 5000 dalton and 15000 dalton carries out.
5. method according to any one of claim 1 to 4, wherein step b) and step n) described at least one diafiltration steps be at least three.
6. method according to any one of claim 1 to 5, wherein step b) described at least one diafiltration steps make to spend mineral water and/or deionized water and/or percolating solution and carry out as solvent.
7. method according to claim 6, wherein said diafiltration uses solvent described in 3 volumes to carry out by every volume concentration protein solution.
8. method according to any one of claim 1 to 7, wherein said steps d) add about 7 grams of fumed silicas by dilute solution often liter described and carry out.
9. method according to any one of claim 1 to 8, wherein said step e) add in described solution carry out by every kilogram of protein being about 100ml to 150ml ethanol.
10. method according to any one of claim 1 to 9, wherein said step g) carry out at the temperature of about 66 DEG C.
11. method according to any one of claim 1 to 10, wherein step m) at least one micro porous filtration described process use the film retained as about 12 microns or 0.6 micron carry out.
12. methods according to any one of claim 1 to 11, the purity of wherein said protein is at least 96%.
13. 1 kinds by the obtainable whey protein concentrate of method according to any one of claim 1 to 12.
14. 1 kinds of whey protein concentrates, every kilogram of protein comprises≤0.5 gram of lactose.
Whey protein concentrate described in 15. any one according to claim 14, the purity of wherein said protein is at least 96%.
16. according to claim 13 to the whey protein concentrate according to any one of 15, it is characterized by the bad smell and/or taste that do not have containing serum proteins typical case.
17. 1 kinds of food, cosmetics or biological technology products, comprise according to claim 13 to the whey protein concentrate according to any one of 16, wherein said product has the concentration of every kilogram of whey protein≤0.5 gram lactose.
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