CN104569346B - A kind ofly to change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal - Google Patents
A kind ofly to change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal Download PDFInfo
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Abstract
Change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal, relate to a kind of evaluation method to grassland soil animal.The invention provides a kind of to change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal.Method of the present invention is: one, Climate change simulation: adopt the standard method of ITEX to use polyethylene board to make open top container, and be arranged in sample ground; Two, grassland utilization mode builds: cradled on the meadow of open top container; Three, vegetation sampling; Four, soil-natural TL sample analysis: soil-natural TL in sample prescription is sampled, by the density of soil animal in data analysis soil, monoid richness and biodiversity index, namely complete and to change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal.The present invention is applied to edaphology field.
Description
Technical field
The present invention relates to a kind of evaluation method to grassland soil animal.
Background technology
Soil-natural TL is ingredient important in geobiont, is the important participant of function of soil ecosystem, and soil-natural TL improves in Decomposition of leaf litter, soil fertility, have material impact in belowground ecosystem material recycle and energy flow.Grassland in Songnen Plain is important animal husbandry production base, but in recent years due to the interference such as human activity and whole world change, the bio-diversity of Grassland in Songnen Plain etc. are subject to havoc.Paid close attention to the investigation of bio-diversity itself about the research of Grassland in Songnen Plain soil animal, and under the background of whole world change, the relation research between soil-natural TL and meadow produce is comparatively weak more in the past.
Summary of the invention
The invention provides a kind of to change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal.
Of the present inventionly a kind ofly change based on open top container simulation of global and in conjunction with grassland utilization mode, the evaluation method of grassland soil animal carried out according to the following steps:
One, Climate change simulation: adopt the standard method of ITEX to use polyethylene board to make open top container, upper terrace with edge area is 1.92 ㎡, and is arranged to by open top container in sample ground;
Two, grassland utilization mode builds: cradled on the meadow of open top container, cradles once if slightly cradle annual August; Severe cradles and cradled once from every two months of June, and the height for the stubble left is 3cm;
Three, vegetation sampling: aboveground plant biomass sampling is carried out to all process in sampling period, and comprise the root system of plant sampling in the 10cm of underground, sample At All Other Times and only the aboveground plant biomass of grass-cutting treatment is sampled;
Four, soil-natural TL sample analysis: soil-natural TL in sample prescription is sampled, the dry funnel method of improvement is used to be separated after sampling, 25 watts of bulbs, be separated 48 hours, the soil animal Sample storage be separated, in the alcohol of 95%, carries out taxonomic identification, and counting of taking pictures, by the density of soil animal in data analysis soil, monoid richness and biodiversity index, namely complete and to change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal.
The present invention includes following beneficial effect:
1, the invention provides simulation original position experimental simulation climate change and the experimental evaluation method that affect soil animal of grassland utilization mode, under whole world change background, soil ecosystem is assessed and is provided new experimental technique.
2, the present invention has extensibility, simultaneously can add the process such as such as fertilising in split plot in the process above, can solve the multiple analog function that Land-Use under Present Global climate change changes.
Embodiment
Embodiment one: a kind of of present embodiment changes based on open top container simulation of global and carry out according to the following steps the evaluation method of grassland soil animal in conjunction with grassland utilization mode:
One, Climate change simulation: adopt the standard method of ITEX to use polyethylene board to make open top container, upper terrace with edge area is 1.92 ㎡, and is arranged to by open top container in sample ground;
Two, grassland utilization mode builds: cradled on the meadow of open top container, cradles once if slightly cradle annual August; Severe cradles and cradled once from every two months of June, and the height for the stubble left is 3cm;
Three, vegetation sampling: aboveground plant biomass sampling is carried out to all process in sampling period, and comprise the root system of plant sampling in the 10cm of underground, sample At All Other Times and only the aboveground plant biomass of grass-cutting treatment is sampled;
Four, soil-natural TL sample analysis: soil-natural TL in sample prescription is sampled, the dry funnel method of improvement is used to be separated after sampling, 25 watts of bulbs, be separated 48 hours, the soil animal Sample storage be separated, in the alcohol of 95%, carries out taxonomic identification, and counting of taking pictures, by the density of soil animal in data analysis soil, monoid richness and biodiversity index, namely complete and to change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal.
Present embodiment comprises following beneficial effect:
1, present embodiments provide for simulation original position experimental simulation climate change and the experimental evaluation method that affect soil animal of grassland utilization mode, under whole world change background, soil ecosystem is assessed and is provided new experimental technique.
2, present embodiment has extensibility, simultaneously can add the process such as such as fertilising in split plot in the process above, can solve the multiple analog function that Land-Use under Present Global climate change changes.
Embodiment two: present embodiment and embodiment one unlike: in described step one, the installation of open top container answers fixation to prevent displacement, and open top container base should keep 1 ~ 3cm distance with ground, make soil animal can external migration in open top container.Other is identical with embodiment one.
Embodiment three: present embodiment and embodiment one or two unlike: in described step one, soil animal is a kind of or wherein several combination in soil earthworm, springtail and acarid.Other is identical with embodiment one or two.
Embodiment four: one of present embodiment and embodiment one to three are unlike described soil animal density=soil animal quantity (head) × Sampling Area (square metre).Other is identical with one of embodiment one to three.
Embodiment five: one of present embodiment and embodiment one to four are unlike species number of individuals in described monoid richness=unit sample area.Other is identical with one of embodiment one to four.
Embodiment six: one of present embodiment and embodiment one to five unlike: described biodiversity index Shannon prestige Nasdaq index number characterizes, concrete formula is: the ratio of the shared overall quantity of sampling quantity of Shannon prestige Nasdaq index number H '=-∑ pilogepi, pi i-th individuality.Other is identical with one of embodiment one to five.
By following verification experimental verification beneficial effect of the present invention:
Dual factors cross-over experiment is implemented in agriculture and animal husbandry ecological Studies station, meadow, Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sc Chang Ling in May, 2011.Experiment adopts six process altogether: 1, contrast; 2, slightly cradle; 3, severe cradles; 4, OTC heats; 5, OTC heats and slightly cradles; 6, OTC heats Hypertention and cradles.
Test one: this test a kind of changes based on open top container simulation of global and be realize according to the following steps in conjunction with the evaluation method of grassland utilization mode to grassland soil animal:
One, Climate change simulation: adopt the standard method of ITEX to use polyethylene board to make open top container, upper terrace with edge area is 1.92 ㎡, and is arranged in sample ground in May, 2011; The installation of open top container answers fixation to prevent displacement, and open top container base should keep 2cm distance with ground, makes soil animal can external migration in open top container;
Two, grassland utilization mode builds: cradled on the meadow of open top container, cradles once if slightly cradle annual August; Severe cradles and cradled once from every two months of June, and the height for the stubble left is 3cm, respectively in June, 2011, August and October, in August, 2012 and October carries out five times and cradles;
Three, vegetation sampling: in August, 2012 carries out aboveground plant biomass sampling to all process, and comprise the root system of plant sampling in the 10cm of underground, the aboveground plant biomass sampling only comprising grass-cutting treatment of sampling At All Other Times;
Four, soil-natural TL sample analysis: six samplings are carried out to soil-natural TL in sample prescription, respectively in August, 2011, October, in June, 2012, August, October and in September, 2013 carry out, the dry funnel method of improvement is used to be separated after sampling, 25 watts of bulbs, be separated 48 hours, the soil animal Sample storage be separated is in the alcohol of 95%, carry out taxonomic identification, and counting of taking pictures, by the density of soil animal in data analysis soil, monoid richness and biodiversity index, namely complete and to change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal,
Wherein, described soil animal density=soil animal quantity (head) × Sampling Area (square metre);
Species number of individuals in described monoid richness=unit sample area;
Described biodiversity index Shannon prestige Nasdaq index number characterizes, and concrete formula is: the ratio of the shared overall quantity of sampling quantity of Shannon prestige Nasdaq index number H '=-∑ pilogepi, pi i-th individuality.
Heat the rising that process causes sample ground soil 5cm temperature, 2011 and experiment sample phase in 2012, do not cradle under heating condition, slightly cradle and to cradle with severe and do not heat not cradling under condition, slightly cradle and compare with severe grass-cutting treatment, soil 5cm temperature-averaging raises 0.344 DEG C.In grass-cutting treatment, because surface vegetation is interfered, soil is easier to be heated by solar radiation, makes grass-cutting treatment show the trend increasing soil 5cm temperature.Because OTC temperature raising device can produce barrier effect to steam transpiration, therefore OTC heats process has increase trend to sample ground 5cm soil moisture, average increase by 1.336%, in like manner grass-cutting treatment reduces sample ground 5cm soil moisture, and slight and severe cradles and reduces by 1.446% and 2.957% respectively.Grass-cutting treatment significantly reduces sample ground Plant aboveground biomass, and heats process intimate plant significantly Plant aboveground biomass, there is not significant reciprocation between cradling and heating.
In each experiment process, the duplicate measurements the results of analysis of variance of springtail individual amount, species number and diversity indices is as shown in table 1.
Table 1
C represents grass-cutting treatment, and W representative heats process.
As shown in Table 1, grass-cutting treatment is to the individual amount significantly increasing springtail group, and the effect heating process is not remarkable.Carry out post-hoc tests to variance analysis, in severe grass-cutting treatment sample ground, the individual amount of springtail is significantly higher than and does not cradle and slight grass-cutting treatment, slight grass-cutting treatment and between not cradling difference not remarkable.To lower three grass-cutting treatment of the condition that heats with do not heat condition three and cradle level and carry out duplicate measurements variance analysis, lower three of the condition that do not heat cradles level and has influence to springtail group individual amount, does not heat severe under condition and cradles springtail group individual amount and be significantly higher than the individual amount slightly cradled.
Claims (4)
1. change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal, it is characterized in that changing based on open top container simulation of global and carrying out according to the following steps the evaluation method of grassland soil animal in conjunction with grassland utilization mode:
One, Climate change simulation: adopt the standard method of ITEX to use polyethylene board to make open top container, upper terrace with edge area is 1.92 ㎡, and is arranged to by open top container in sample ground;
Two, grassland utilization mode builds: cradled on the meadow of open top container, cradles once if slightly cradle annual August; Severe cradles and cradled once from every two months of June, and the height for the stubble left is 3cm;
Three, vegetation sampling: aboveground plant biomass sampling is carried out to all process in sampling period, and comprise the root system of plant sampling in the 10cm of underground, sample At All Other Times and only the aboveground plant biomass of grass-cutting treatment is sampled;
Four, soil-natural TL sample analysis: soil-natural TL in sample prescription is sampled, the dry funnel method of improvement is used to be separated after sampling, 25 watts of bulbs, be separated 48 hours, the soil animal Sample storage be separated, in the alcohol of 95%, carries out taxonomic identification, and counting of taking pictures, by the density of soil animal in data analysis soil, monoid richness and biodiversity index, namely complete and to change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal.
2. according to claim 1ly a kind ofly to change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal, it is characterized in that the installation of open top container in described step one answers fixation to prevent displacement, and open top container base should keep 1 ~ 3cm distance with ground, make soil animal can external migration in open top container.
3. according to claim 1ly a kind ofly change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal, it is characterized in that species number of individuals in described monoid richness=unit sample area.
4. according to claim 1ly a kind ofly to change based on open top container simulation of global and in conjunction with the evaluation method of grassland utilization mode to grassland soil animal, it is characterized in that described biodiversity index Shannon prestige Nasdaq index number characterizes, concrete formula is: Shannon prestige Nasdaq index number H '=-∑ pilog
epi, pi are the ratio of the shared overall quantity of sampling quantity of i-th individuality.
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| CN101805614A (en) * | 2010-03-29 | 2010-08-18 | 天津师范大学 | Method for improving photosynthetic capacity of tall fescue by adopting saline soil filled with waste crumb rubber |
| DE202011102872U1 (en) * | 2011-06-16 | 2011-09-27 | ecoTech Umwelt-Meßsysteme GmbH | Elution column with replaceable filter plates and quick release devices |
| CN102875220A (en) * | 2012-09-11 | 2013-01-16 | 中国科学院沈阳应用生态研究所 | Special formula fertilizer for northern mowing grasslands and use method of special formula fertilizer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101805614A (en) * | 2010-03-29 | 2010-08-18 | 天津师范大学 | Method for improving photosynthetic capacity of tall fescue by adopting saline soil filled with waste crumb rubber |
| DE202011102872U1 (en) * | 2011-06-16 | 2011-09-27 | ecoTech Umwelt-Meßsysteme GmbH | Elution column with replaceable filter plates and quick release devices |
| CN102875220A (en) * | 2012-09-11 | 2013-01-16 | 中国科学院沈阳应用生态研究所 | Special formula fertilizer for northern mowing grasslands and use method of special formula fertilizer |
Non-Patent Citations (2)
| Title |
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| 刈割和封育对松嫩草原碱化羊草草地土壤跳虫群落的影响;吴东辉 等;《草 业 学 报》;20081231;第17卷(第5期);117-123 * |
| 刈割活动对松嫩草原碱化羊草草地土壤线虫群落的影响;吴东辉 等;《生物多样性》;20071231;第15卷(第2期);180-187 * |
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