CN104569275B - The method simultaneously measuring ursolic acid and Fructus Schisandrae Chinensis the first and second C primes monomer component concentration - Google Patents
The method simultaneously measuring ursolic acid and Fructus Schisandrae Chinensis the first and second C primes monomer component concentration Download PDFInfo
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Abstract
The invention discloses a kind of method measuring ursolic acid and Fructus Schisandrae Chinensis the first and second C primes monomer component concentration simultaneously, belong to chromatograph material.The present invention prepares Fructus Corni and Fructus Schisandrae Chinensis biased sample and testing sample negative control solution respectively, after pretreatment, is acetonitrile in mobile phase:0.1% phosphate aqueous solution=67:33, volume flow 0.8 1.2 mL/min；Ultraviolet detection wavelength 190 220 nm；Under the conditions of during 25 40 DEG C of column temperature, it is operated preparation and the chromatographic isolation of four kinds of monomer components and the mensure of content of curve.Achieve in relative short time, under the conditions of Same Wavelength chromatography experiment, detect simultaneously and be kept completely separate ursolic acid and the concentration of Fructus Schisandrae Chinensis first, second, four kinds of monomer components of C prime, baseline is steady, peak type is symmetrically attractive in appearance, easy to be quick, separating degree is high, and sensitivity is high, reproducible, accurately and reliably, the response rate is high, there is provided a kind of good Quality Control detection meanss for data.
Description
Technical field
The present invention relates to chromatograph Material Field, specifically one kind measure ursolic acid and Fructus Schisandrae Chinensis the first and second the third simultaneously
The method of plain monomer component concentration.
Background technology
Chinese medicine especially natural Chinese medicine plays an important role in thousand of years mankind multiply, survive, developing, and the five tastes
Son, Fructus Corni are exactly medical material more important among these.Fructus Schisandrae Chinensis are perennial fallen leaves lianas, belong to Magnoliaceae, and this medicine function is neat
Entirely, the five tastes all have, therefore have the title of Fructus Schisandrae Chinensis.In Chinese medicinal material the five tastes have concurrently real belong to exclusive, and it is to the deficiency of YIN and yang deficiency all
There is unique curative effect.Fructus Schisandrae Chinensis have functions that astringe the lung nourishing kidney, the arresting sweating that promotes the production of body fluid, arresting seminal emission antidiarrheal, mind tranquilizing and the heart calming.Fructus Schisandrae Chinensis mainly contain
The main components such as lignanoid, volatile oil, organic acid, triterpene, sesquiterpene, wherein Lignanoids compounds mainly include Fructus Schisandrae Chinensis first
Element, schisandrin B and schisandrin C etc..Modern pharmacology shows, Fructus Schisandrae Chinensis first, second, C prime have many pharmacology and live
Property, such as Central nervous system has sedation, too high to chronic hepatitiss, neurasthenia, gastric acidity, and the disease such as ulcer has
There is good clinical efficacy.Fructus Corni is deciduous tree or dungarunga, and drying and ripening sarcocarp is used as medicine.Fructus Corni has tonification liver
The astringent or styptic treatment for spontaneous sweating effect of kidney, convergence.Fructus Corni mainly contains meliatin, cornin, the new glycosides of Fructus Corni, ursolic acid, oleanolic acid etc.
Multiple element.Modern pharmacology shows, ursolic acid has reduction blood glucose, and defying age and immunomodulating etc. act on.As can be seen here, Bearss
Fruit acid and Fructus Schisandrae Chinensis first, second, C prime are Chinese medicine Fructus Corni and Fructus Schisandrae Chinensis play the most key active monomer component of pharmacological action.
Therefore, it is heavy to closing for finding the Quality Control detection method simultaneously detecting Fructus Schisandrae Chinensis first, second, C prime and ursolic acid to ensureing the quality of the pharmaceutical preparations
Want.
In prior art, when detecting Fructus Schisandrae Chinensis first, second, C prime with reversed phase high-performance liquid chromatography, adopt gradient more simultaneously
Elution method, selection 250nm is absorbing wavelength, due to the ratio of mobile phase to be converted in experimentation, can cause baseline fluctuation, spirit
Sensitivity reduces, the accuracy of impact experimental result；And majority appearance, after 30min, causes experimental period long.
Content of the invention
The present invention is exactly that linear gradient elution method causes when detecting Fructus Schisandrae Chinensis first, second, C prime simultaneously in order to overcome in prior art
Baseline fluctuation, sensitivity decrease, and ursolic acid and Fructus Schisandrae Chinensis first, second, four kinds of active monomer components of C prime can not be detected simultaneously
Shortcoming, and a kind of isocratic elution method providing measures the side of ursolic acid and Fructus Schisandrae Chinensis the first and second C primes monomer component concentration simultaneously
Method.
The present invention is to realize according to technical scheme below.
A kind of method simultaneously measuring ursolic acid and Fructus Schisandrae Chinensis the first and second C primes monomer component concentration, comprises the following steps：
A. the pretreatment of sample
Fructus Schisandrae Chinensis, the Fructus Corni drying of medical material containing ursolic acid, to constant weight, are crossed 40 mesh sieves after pulverizing, are claimed respectively by ratio of weight and the number of copies
Take 10 parts of the Fructus Corni of medical material containing ursolic acid, 1 part of Fructus Schisandrae Chinensis, after mixing, add 10 times amount dehydrated alcohol；Ultrasonic extraction sucking filtration,
Filtrate crosses 0.22 μm of microporous filter membrane, sample size 3-20 μ L；
Testing sample lacks the preparation of the Fructus Corni negative control solution of medical material containing ursolic acid：Essence claims 1 part of Chinese Magnolivine Fruit, system
Standby, filter, sample size ibid；
Testing sample lacks the preparation of Fructus Schisandrae Chinensis negative control solution：Essence claims 10 parts of Asiatic Cornelian Cherry Fruits of medical material containing ursolic acid, system
Standby, filter, sample size ibid；
B. high performance liquid chromatography operating condition
Agilent 1260 type high performance liquid chromatograph；Chromatographic column：150 × 46 mm, 5 μm, ZORBAX SB-C18；
Mobile phase is acetonitrile 67 70%:0.1% phosphate aqueous solution 33 30%, volume flow is 0.8-1.2 mL/min；Ultraviolet detection ripple
A length of 190-220 nm；25-40 DEG C of column temperature；
The condition of ultrasonic extraction is 250W, 40KHz, 20 DEG C, and ultrasonic time is 30min；
C. the preparation of working curve
Precision weighs ursolic acid reference substance 1.36 mg, A prime reference substance 1.0 mg, schisandrin B reference substance 1.36 respectively
Mg, schisandrin C reference substance 0.82 mg, are made into the dehydrated alcohol mixed reference substance solution of 1mL, and carry out doubling dilution, altogether
Obtain 8 mass concentrations of four kinds of monomer mixing reference substance dilute solutions, take the peak area that rear 7 mass concentrations sample introduction measure
Draw standard curve with corresponding concentration；Each mass concentration liquid crosses 0.22 μm of microporous filter membrane respectively, and sample introduction 10 μ L carries out chromatograph
Analysis, with peak areaATo concentrationCCarry out linear regression, obtain four kinds of monomer component working curves simultaneously；
D. the mensure of the chromatographic isolation of four kinds of monomer components and content
Fructus Schisandrae Chinensis through pretreatment and the Fructus Corni sample of medical material containing ursolic acid are carried out upper machine mensure, four kinds of monomer components
Carry out chromatographic isolation, and according to each corresponding peak area of Chinese medicine monomer composition in testing sample by corresponding working curve respectively
Calculate the content of four kinds of monomer components.
The invention has the beneficial effects as follows：
The present invention can be in relative short time（25min）Using isocratic elution method, in Same Wavelength chromatography experiment condition
Under, detect simultaneously and be kept completely separate have valuable pharmacological activity Chinese medicine monomer composition ursolic acid and Fructus Schisandrae Chinensis first, second, third
Element.Measure the concentration of four kinds of monomer components using the method for the present invention, steadily, peak type is symmetrically attractive in appearance for baseline simultaneously, easy to be quick,
Separating degree is high, and sensitivity is high, reproducible, and accurately and reliably, the response rate is high for data, there is provided a kind of good Quality Control detection handss
Section.
Brief description
Fig. 1 is control sample high-efficient liquid phase chromatogram of the present invention；
Fig. 2 is testing sample high-efficient liquid phase chromatogram of the present invention；
Fig. 3 is the negative control solution high-efficient liquid phase chromatogram that testing sample of the present invention lacks Fructus Schisandrae Chinensis；
Fig. 4 lacks the negative control solution high-efficient liquid phase chromatogram of Fructus Corni for testing sample of the present invention.
Specific embodiment
Below in conjunction with the accompanying drawings and embodiment the present invention is described in detail.
1. instrument and material
2.1 instrument
Agilent 1260 type high performance liquid chromatograph, including quaternary pump, G1311C, automatic sampler, G1329B, VWD
Detector, G1314F, chromatographic work station, Anjelen Sci. ＆ Tech. Inc；
AE240 precise electronic analytical balance, prunus mume (sieb.) sieb.et zucc. Teller-Shanghai support benefit instrument；
Superpure water machine, French MILLIPORE；
Universalpulverizer, Beijing Zhong Xing great achievement Instrument Ltd. manufactures；
Supersonic extractors, Ningbo Xin Yi biotech inc；
Electric drying oven with forced convection, Tianjin three hydroscience Instrument Ltd..
2.2 material
Fructus Schisandrae Chinensis first, second, C prime reference substance, Nat'l Pharmaceutical ＆ Biological Products Control Institute, mass fraction is no less than 99.9%, batch
Numbers 20130306；
Ursolic acid, Nat'l Pharmaceutical ＆ Biological Products Control Institute, mass fraction is no less than 99.9%, lot number 20130406；
Acetonitrile（Chromatographically pure）, German Merk company；
Phosphoric acid（Chromatographically pure）, fine chemistry industry institute is recovered in Tianjin；
Experimental water is secondarily purified water, and Tianjin City No.1 Central Hospital pharmaceutical factory provides.
2. experimental technique and result
The pretreatment of 2.1 samples
It is dried to constant weight at Fructus Schisandrae Chinensis, 60 DEG C of the Fructus Corni of medical material containing ursolic acid, after pulverizing, crosses 40 mesh sieves, precision weighs respectively
Medical material containing ursolic acid Fructus Corni 1g, Fructus Schisandrae Chinensis 0.1g, the powder of mixing is poured in 25ml conical flask, adds 1mL dehydrated alcohol.
Under 250W, 40KHz, 20 DEG C of experiment condition, supersound extraction 30min, the ethanol solution that sucking filtration is extracted, filtrate cross 0.22 μm micro-
Hole filter membrane, sample introduction 10 μ L.
Testing sample lacks the preparation of the Fructus Corni negative control solution of medical material containing ursolic acid：Essence claims Chinese Magnolivine Fruit 0.1g, will
The powder of mixing is poured in conical flask, adds 1mL dehydrated alcohol；Under 250W, 40KHz, 20 DEG C of experiment condition, supersound extraction
30min, the ethanol solution that sucking filtration is extracted, filtrate crosses 0.22 μm of microporous filter membrane, sample introduction 10 μ L.
Testing sample lacks the preparation of Fructus Schisandrae Chinensis negative control solution：Essence claims the Asiatic Cornelian Cherry Fruit 1g of medical material containing ursolic acid, will mix
The powder closing is poured in conical flask, adds 10mL dehydrated alcohol；Under 250W, 40KHz, 20 DEG C of experiment condition, supersound extraction
30min, the ethanol solution that sucking filtration is extracted, filtrate crosses 0.22 μm of microporous filter membrane, sample introduction 10 μ L.
2.2 chromatographic condition
Agilent 1260 type high performance liquid chromatograph；Chromatographic column：ZORBAX SB-C18（150 × 46 mm, 5 μm）Color
Spectrum post.Mobile phase is acetonitrile:0.1% phosphate aqueous solution=67:33, volume flow 1.0 mL/min；Ultraviolet detection wavelength is 210
nm；30 DEG C of column temperature；Sample size 10 μ L.
The preparation of 2.3 working curves
Precision weighs ursolic acid reference substance 1.36 mg, A prime reference substance 1.0 mg, schisandrin B reference substance 1.36 respectively
Mg, schisandrin C reference substance 0.82 mg, are made into the dehydrated alcohol mixed reference substance solution of 1mL, and carry out doubling dilution, obtain
To 8 mass concentrations of four kinds of monomer mixing reference substance dilute solutions, take rear 7 mass concentrations and peak area that sample introduction measures with
Corresponding concentration obtains the working curve of four kinds of monomer components simultaneously.
2.3.1 ursolic acid working curve
In each 8 mass concentrations of the four kinds of monomer mixing reference substance dilute solutions obtaining, obtain ursolic acid dilution molten
The mass concentration of liquid is 680,340,170,85,42.5,21.25,10.625,5.313 mg/L；Rear 7 mass concentrations are taken to go forward side by side
The peak area that sample measures draws standard curve with corresponding concentration；Each mass concentration liquid crosses 0.22 μm of microporous filter membrane, sample introduction respectively
10 μ L carry out chromatography；With peak areaATo concentrationC（mg/L）Carry out linear regression, obtain ursolic acid working curve：A=
6.135C+18.700, r=0.9995；Ursolic acid is in good line with peak area between mass concentration 340～5.313 mg/L
Sexual intercourse；According to signal to noise ratio 3/1, ursolic acid lowest detection mass concentration is 0.025 mg/L.
2.3.2 deoxyschizandrin working curve
In each 8 mass concentrations of the four kinds of monomer mixing reference substance dilute solutions obtaining, obtain deoxyschizandrin dilute
The mass concentration releasing solution is 500,250,125,62.5,31.25,15.625,7.812,3.906 mg/L；Take rear 7 quality
The peak area of concentration sample introduction mensure and corresponding concentration draw standard curve；Each mass concentration liquid crosses 0.22 μm of micropore respectively
Filter membrane, sample introduction 10 μ L carries out chromatography；With peak areaATo concentrationC（mg/L）Carry out linear regression, obtain deoxyschizandrin work
Make curve：A=79.196C+196.449, r=0.9994；Deoxyschizandrin between mass concentration 250～3.906 mg/L with peak
Area is in good linear relationship；According to signal to noise ratio 3/1, the lowest detection mass concentration of deoxyschizandrin is 0.016 mg/L.
2.3.3 schisandrin B working curve
In each 8 mass concentrations of the four kinds of monomer mixing reference substance dilute solutions obtaining, obtain schisandrin B dilute
The mass concentration releasing solution is 680,340,170,85,42.5,21.25,10.625,5.313 mg/L；Take rear 7 mass concentrations
And the peak area that sample introduction records draws standard curve with corresponding concentration；Each mass concentration liquid crosses 0.22 μm of microporous filter membrane respectively,
Sample introduction 10 μ L carries out chromatography；With peak areaATo concentrationC（mg/L）Carry out linear regression, obtain schisandrin B work bent
Line：A=22.085C+66.829, r=0.9995；Schisandrin B between mass concentration 340～5.313 mg/L with peak area
In good linear relationship；According to signal to noise ratio 3/1, the lowest detection mass concentration of schisandrin B is 0.019 mg/L.
2.3.4 schisandrin C working curve
In each 8 mass concentrations of the four kinds of monomer mixing reference substance dilute solutions obtaining, obtain schisandrin C dilute
The mass concentration releasing solution is 410,205,102.5,51.25,25.625,12.810,6.450,3.225 mg/L；Take latter 7
The peak area that mass concentration sample introduction record draws standard curve with corresponding concentration；Each mass concentration liquid crosses 0.22 μm respectively
Microporous filter membrane, sample introduction 10 μ L carries out chromatography；With peak areaATo concentrationC（mg/L）Carry out linear regression, obtain Fructus Schisandrae Chinensis third
Plain working curve：A=23.106C+43.569, r=0.9995；Schisandrin C is between mass concentration 205～3.225 mg/L
It is in good linear relationship with peak area；According to signal to noise ratio 3/1, the lowest detection mass concentration of schisandrin C is 0.020
mg/L.
2.4 chromatographic isolation
Respectively chromatographic isolation is carried out to the ursolic acid in sample to be tested and Fructus Schisandrae Chinensis first, second, C prime, result see Fig. 1,2（Figure
In：1：Deoxyschizandrin；2：Schisandrin B；3：Schisandrin C；4：Ursolic acid）.It can be seen that, the separation of each component all can reach
Baseline separation, peak type is symmetrical, and separating degree is good, and no substantially impurity peaks interference.The retention time of ursolic acid is 23.003 min；Five
The retention time of taste A prime is 10.401 min；The retention time of schisandrin B is 13.329 min；Schisandrin C
Retention time is 15.388 min.
Do the negative control experiment that sample to be tested lacks Fructus Schisandrae Chinensis and Fructus Corni respectively respectively, result is shown in Fig. 3 and 4.It can be seen that,
Fig. 3 is not detected by the chromatographic peak of this three kinds of monomer components in Fructus Schisandrae Chinensis first, second, the corresponding retention time of C prime；Fig. 4 is in Folium Vaccinii vitis-idaeae
It is not detected by its chromatographic peak in the corresponding retention time of acid.Illustrate that do not interfere with peak occurs when detecting these four monomer components.
2.5 precision test（RSD）
To deoxyschizandrin concentration be 15.620 mg/L, schisandrin B concentration be 21.25 mg/L, schisandrin C
The same mixing contrast solution that concentration is 25.625 mg/L, Folium Vaccinii vitis-idaeae acid concentration is 21.25 mg/L, continuous sample introduction 5 times, respectively
Record the chromatographic peak area numerical value of corresponding monomer component, and be statistically analyzed, measuring ursolic acid RSD is 1.86%, Fructus Schisandrae Chinensis first
Plain RSD is 1.76%, schisandrin B RSD is 1.69%, schisandrin C RSD is 1.80%（N=5）.
2.6 replica test
Preparation method according to above-mentioned product to be tested prepares 6 parts of product to be tested solution simultaneously, and sample introduction 1 time, records corresponding list respectively
The chromatographic peak area numerical value of body composition, and it is statistically analyzed method, ursolic acid RSD is that 1.56%, deoxyschizandrin RSD is
1.77%th, schisandrin B RSD be 1.66%, schisandrin C RSD be 1.49%（N=6）.
2.7 mean sample recovery rate tests
In 0.9 mL sample to be tested solution（Detection ursolic acid mass concentration is 1.635 mg/L；Deoxyschizandrin is
1.361mg/L；Schisandrin B is 9.238mg/L；Schisandrin C is 0.953mg/L）It is separately added into 0.1 mL mixing comparison
Product solution, ursolic acid mass concentration is respectively 10.625,21.25,42.5 mg/L；Deoxyschizandrin mass concentration is respectively
7.812、15.620、31.25 mg/L；Schisandrin B mass concentration is respectively 10.625,21.25,42.5 mg/L；Fructus Schisandrae Chinensis
C prime mass concentration is respectively 6.406,12.813,25,625 mg/L.Sample introduction records its mass concentration, calculates its response rate, knot
Fruit ursolic acid average recovery rate is 101.16%, RSD is 1.36%；Deoxyschizandrin average recovery rate for 100.79%, RSD is
1.41%；Schisandrin B average recovery rate is 101.09%, RSD is 1.49%；Schisandrin C average recovery rate is 101.26%,
RSD is 1.31%.
2.8 stability test
Same sample to be tested is measured related corresponding chromatograph value in 0,4,8,12,24,48 h respectively.Result ursolic acid
RSD is 1.56%；The RSD of deoxyschizandrin is 1.61%；The RSD of schisandrin B is 1.39%；The RSD of schisandrin C is
1.60%.In display sample to be tested 48 h, sample is stable.
The mensure of 2.9 sample to be tested contents
Record the amount of ursolic acid and Fructus Schisandrae Chinensis first, second, C prime in 5 samples to be tested respectively, the results are shown in Table 1.
Ursolic acid and Fructus Schisandrae Chinensis the first and second C prime assay (mg/g) in table 1. sample to be tested
In sum, the application present invention can be in relative short time（25min）Using isocratic elution method, in Same Wavelength
Under the conditions of chromatography experiment, detect simultaneously and be kept completely separate the Chinese medicine monomer composition ursolic acid with valuable pharmacological activity and the five tastes
Sub- first, second, C prime.Measure the concentration of four kinds of monomer components using the method for the present invention, steadily, peak type is symmetrically attractive in appearance for baseline, letter
Just quick, separating degree is high, and sensitivity is high, reproducible, and accurately and reliably, the response rate is high, there is provided a kind of good Quality Control for data
Detection meanss.
Claims (5)
1. a kind of method simultaneously measuring ursolic acid and Fructus Schisandrae Chinensis the first and second C primes monomer component concentration it is characterised in that：Including with
Lower step：
A. the pretreatment of sample
Fructus Schisandrae Chinensis, the Fructus Corni drying of medical material containing ursolic acid, to constant weight, are crossed 40 mesh sieves after pulverizing, are weighed respectively by ratio of weight and the number of copies and contain
10 parts of ursolic acid medical material Fructus Corni, 1 part of Fructus Schisandrae Chinensis, add 10 times amount dehydrated alcohol after mixing；Ultrasonic extraction sucking filtration, filtrate
Cross 0.22 μm of microporous filter membrane, sample size 10 μ l；
Testing sample lacks the preparation of the Fructus Corni negative control solution of medical material containing ursolic acid：Essence claims 1 part of Chinese Magnolivine Fruit, preparation, mistake
Filter, sample size are ibid；
Testing sample lacks the preparation of Fructus Schisandrae Chinensis negative control solution：Essence claims 10 parts of Asiatic Cornelian Cherry Fruits of medical material containing ursolic acid, preparation, mistake
Filter, sample size are ibid；
B. high performance liquid chromatography operating condition
Agilent1260 type high performance liquid chromatograph；Chromatographic column：150 × 4.6mm, 5 μm, ZORBAX SB-C18；Mobile phase is
Acetonitrile:0.1% phosphate aqueous solution=67:33, volume flow is 0.8-1.2mL/min；Ultraviolet detection wavelength is 210nm；Column temperature 25-
40℃；
The condition of ultrasonic extraction is 250W, 40KHz, 20 DEG C, and ultrasonic time is 30min；
C. the preparation of working curve
Precision weighs ursolic acid reference substance 1.36mg, deoxyschizandrin reference substance 1.0mg, schisandrin B reference substance respectively
1.36mg, schisandrin C reference substance 0.82mg, are made into the dehydrated alcohol mixed reference substance solution of 1mL, and carry out doubling dilution,
8 mass concentrations of four kind monomer mixing reference substance dilute solutions are obtained, take the peak area that rear 7 mass concentration sample introductions measure
Draw standard curve with corresponding concentration；Each mass concentration liquid crosses 0.22 μm of microporous filter membrane respectively, and sample introduction 10 μ L carries out chromatograph and divides
Analysis, carries out linear regression with peak area A to concentration C, obtains four kinds of monomer component working curves simultaneously；The wherein minimum inspection of ursolic acid
Mass metering concentration is 0.025mg/L, and the lowest detection mass concentration of deoxyschizandrin is 0.016mg/L, and schisandrin B is
Low detection mass concentration is 0.019mg/L, and the lowest detection mass concentration of schisandrin C is 0.020mg/L；
D. the mensure of the chromatographic isolation of four kinds of monomer components and content
Fructus Schisandrae Chinensis through pretreatment and the Fructus Corni sample of medical material containing ursolic acid are carried out upper machine mensure, four kinds of monomer components are carried out
Chromatographic isolation, and calculated respectively by corresponding working curve according to each corresponding peak area of Chinese medicine monomer composition in testing sample
Go out the content of four kinds of monomer components.
2. a kind of side simultaneously measuring ursolic acid and Fructus Schisandrae Chinensis the first and second C primes monomer component concentration according to claim 1
Method it is characterised in that：In four kinds of monomer mixing reference substance dilute solutions, 8 mass concentrations obtaining ursolic acid dilute solution are divided
Not Wei 680,340,170,85,42.5,21.25,10.625,5.313mg/L；Take the peak that rear 7 mass concentrations sample introduction measure
Area and corresponding concentration obtain ursolic acid working curve：A=6.135C+18.700, r=0.9995；
Ursolic acid is in good linear relationship with peak area between mass concentration 340～5.313mg/L；According to signal to noise ratio 3/1,
Ursolic acid lowest detection mass concentration is 0.025mg/L.
3. a kind of side simultaneously measuring ursolic acid and Fructus Schisandrae Chinensis the first and second C primes monomer component concentration according to claim 1
Method it is characterised in that：In four kinds of monomer mixing reference substance dilute solutions, 8 quality obtaining deoxyschizandrin dilute solution are dense
Degree be respectively 500,250,125,62.5,31.25,15.625,7.812,3.906mg/L；Take rear 7 mass concentrations and sample introduction is surveyed
Fixed peak area and corresponding concentration obtain deoxyschizandrin working curve：A=79.196C+196.449, r=0.9994；
Deoxyschizandrin is in good linear relationship with peak area between mass concentration 250～3.906mg/L；According to signal to noise ratio
3/1, the lowest detection mass concentration of deoxyschizandrin is 0.016mg/L.
4. a kind of side simultaneously measuring ursolic acid and Fructus Schisandrae Chinensis the first and second C primes monomer component concentration according to claim 1
Method it is characterised in that：In four kinds of monomer mixing reference substance dilute solutions, 8 quality obtaining schisandrin B dilute solution are dense
Degree be respectively 680,340,170,85,42.5,21.25,10.625,5.313mg/L；Take rear 7 mass concentrations and sample introduction measures
Peak area and corresponding concentration obtain schisandrin B working curve：A=22.085C+66.829, r=0.9995；
Schisandrin B is in good linear relationship with peak area between mass concentration 340～5.313mg/L；According to signal to noise ratio
3/1, the lowest detection mass concentration of schisandrin B is 0.019mg/L.
5. a kind of side simultaneously measuring ursolic acid and Fructus Schisandrae Chinensis the first and second C primes monomer component concentration according to claim 1
Method it is characterised in that：In four kinds of monomer mixing reference substance dilute solutions, 8 quality obtaining schisandrin C dilute solution are dense
Degree be respectively 410,205,102.5,51.25,25.625,12.810,6.450,3.225mg/L；Rear 7 mass concentrations are taken to go forward side by side
The peak area that sample measures and corresponding concentration obtain schisandrin C working curve：A=23.106C+43.569, r=0.9995；
Schisandrin C is in good linear relationship with peak area between mass concentration 205～3.225mg/L；According to signal to noise ratio
3/1, the lowest detection mass concentration of schisandrin C is 0.020mg/L.
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