CN104568934A - Application of nanometer cobaltosic oxide used as peroxide enzyme mimic for measuring concentration of hydrogen peroxide - Google Patents
Application of nanometer cobaltosic oxide used as peroxide enzyme mimic for measuring concentration of hydrogen peroxide Download PDFInfo
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- CN104568934A CN104568934A CN201510001844.2A CN201510001844A CN104568934A CN 104568934 A CN104568934 A CN 104568934A CN 201510001844 A CN201510001844 A CN 201510001844A CN 104568934 A CN104568934 A CN 104568934A
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Abstract
The invention relates to application of nanometer cobaltosic oxide (Co3O4) used as a peroxide enzyme mimic for measuring the concentration of hydrogen peroxide; nanometer cobaltosic oxide used as the peroxide enzyme mimic has peroxidase-like activity, and further comprises various sizes of nanometer cobaltosic oxide particles; the particle diameter of nanometer cobaltosic oxide exceeds 9 nm; a cobaltosic oxide nanometer material can be used for catalyzing degradation of a phenolic substance, and has potential for being applied to printing and dyeing wastewater treatment. According to the application provided by the invention, the peroxide enzyme mimic function of nanometer cobaltosic oxide is found for the first time, so that nanometer cobaltosic oxide can be used as the peroxide enzyme mimic for measuring the content of hydrogen peroxide; compared with HRP, nanometer cobaltosic oxide used as a catalyst is higher in stability and reusability, and more efficient in peroxide enzyme mimic catalytic activity; without the presence of hydrogen peroxide, nanometer cobaltosic oxide can also be used for catalyzing color developing agents, and used as an enzyme mimic.
Description
Technical field
The present invention relates to a kind of nano-cobaltic-cobaltous oxide (Co
3o
4) measure the application of hydrogen peroxide as Mimetic Peroxidase.The invention belongs to nano biological field of chemical detection.
Background technology
Biology enzyme is the protein that a class has catalysis, and a small amount of exist can fast reaction speed greatly; Sometimes also can participate in reaction, but before and after reaction, itself is unchanged; Have very high catalytic efficiency, react single-minded and reaction conditions gentle.But native enzyme changeableness inactivation, expensive, purification difficult, the shortcomings such as storage and use cost height, make the research of analogue enztme become the task of top priority.
From Late Cambrian magnetic ferroferric oxide nano-particles (Fe in 2007
3o
4nPs) after possessing the catalytic activity of class peroxidase, i.e. Fe
3o
4nPs can catalysis H
2o
2with the substrate 3,3 ' of horseradish peroxidase, 5., the reactions such as 5 '-tetramethyl benzidine (TMB), diazoaminobenzene (DAB), generate identical reaction product, show the catalytic action similar with HRP.After this, the various nano particle with class Mimetic Peroxidase characteristic is synthesized sign one after another, as carbon multi-wall nano tube loaded ferroferric oxide nano granules, the golden nanometer particle (GQDAuNPs) of graphene quantum dot functionalization, nano-metal-oxide (Chinese invention patent: nano cupric oxide analogue enztme and the method as Mimetic Peroxidase mensuration hydrogen peroxide thereof, the patent No.: 201010519693; A kind of CsxWO
3the application of class Mimetic Peroxidase, the patent No.: 201410220658) etc.Compared to HRP, it is high that nano-metal-oxide has stability as catalyzer, reusable, and efficient Mimetic Peroxidase catalytic activity.
The invention provides a kind of nano-cobaltic-cobaltous oxide particle is detected hydrogen oxide method as Mimetic Peroxidase.
Summary of the invention
For overcoming the deficiencies in the prior art, the object of the invention is to provide a kind of nano-cobaltic-cobaltous oxide class Mimetic Peroxidase for being detected the new method of hydrogen oxide.This nano-cobaltic-cobaltous oxide can produce the hydroxyl radical free radical of strong oxidizing property by catalyzing hydrogen peroxide, efficient catalytic 3,3 ', 5,5 '-tetramethyl biphenyl amine hydrochlorate (TMB), 2,2 '-hydrazine-bis-(3-ethyl benzo match azoles quinoline-6-sulfonic acid)-diamine salts (ABTS) etc. produces chromogenic reaction.Have the reaction time short, colour developing is fast, and catalytic effect and applicability are all higher than the advantage of natural peroxide enzyme.
A kind of nano-cobaltic-cobaltous oxide measures the application of concentration of hydrogen peroxide as Mimetic Peroxidase, it is characterized in that: nano-cobaltic-cobaltous oxide is as class Mimetic Peroxidase, there is the activity of class peroxidase, its particle diameter is not limited to 9nm, also comprises the nano-cobaltic-cobaltous oxide particle of other sizes.
H can be detected
2o
2the method of content, detects and is limited to 10
-5.
Catalyzing hydrogen peroxide oxidation TMB hydrochloride (TMB), becomes blueness from colourless.
Catalyzing hydrogen peroxide oxidation 2,2 '-hydrazine-bis-(3-ethyl benzo match azoles quinoline-6-sulfonic acid)-diamine salts (ABTS), becomes green from colourless.
Its buffer solution is sodium acetate buffer solution.
Nano-cobaltic-cobaltous oxide has under existing without hydrogen peroxide, still can the ability of phenol substance catalytic, except being excellent Mimetic Peroxidase, or the excellent catalyst of wastewater treatment.
1, the synthesis of nano-cobaltic-cobaltous oxide
Metal-nitrate solutions is dropwise instilled sodium carbonate (Na
2cO
3) (Na in solution
2cO
3amount just make metal nitrate precipitate completely), 50 DEG C of reaction 1h, deionized water washing is to neutral, and after drying, roasting 3h, can obtain nano-metal-oxide.
2, TMB is as developer, measures H
2o
2concentration
In solution, add certain density cobaltosic oxide solution and TMB solution, then add the H of different amount
2o
2, make H
2o
2form concentration gradient.Finally add sodium acetate buffer solution, make the final volume of solution to be measured be 200ml.Use microplate reader to measure the time dependent light absorption value of solution, detectability can reach 10
-5mol/L.With this curve for typical curve, H can be inferred according to light absorption value
2o
2concentration.
3, ABTS is as developer, measures H
2o
2concentration
In solution, add a certain amount of cobaltosic oxide solution and ABTS solution, then add the H of different amount
2o
2, make H
2o
2form concentration gradient, add sodium acetate buffer solution (pH=4.0), make the final volume of solution to be measured be 200ml.Microplate reader is used to measure the light absorption value of solution.With this curve for typical curve, H can be inferred according to light absorption value
2o
2concentration.
As shown from the above technical solution, nano-cobaltic-cobaltous oxide of the present invention has the function of peroxidase, can be oxidized peroxidase substrate by catalyzing hydrogen peroxide, be a kind of novel Mimetic enzyme of peroxidase, can be used for the detection of hydrogen peroxide.Simultaneously cobaltosic oxide still can catalysis TMB and ABTS under without hydrogen peroxide existent condition, and catalytic reaction is violent, and reaction rate very soon, is excellent nano enzyme.
The invention has the advantages that:
(1) Late Cambrian nano-cobaltic-cobaltous oxide of the present invention has Mimetic Peroxidase function, can be used for the content being detected hydrogen oxide;
(2) nano-cobaltic-cobaltous oxide prepared of the present invention is as catalyzer compared to HRP, has stability high, reusable, and Mimetic Peroxidase catalytic activity efficiently;
(3) the present invention finds that without under hydrogen peroxide existence, nano-cobaltic-cobaltous oxide equally can catalyzed coloration agent, uses as analogue enztme;
(4) the preparation method's technique in the present invention is simple, workable, can meet further and produce and application.
Accompanying drawing explanation
Fig. 1 is the XRD phenogram of nano-cobaltic-cobaltous oxide of the present invention.
Fig. 2 is embodiment 2 take TMB as developer, and nano-cobaltic-cobaltous oxide is detected hydrogen oxide figure.
Fig. 3 is embodiment 3 take ABTS as developer, and nano-cobaltic-cobaltous oxide is detected hydrogen oxide figure.
Embodiment
Below by way of specific embodiment, technical scheme of the present invention is further described.Following embodiment further illustrates of the present invention, and do not limit the scope of the invention.
embodiment 1;
The synthesis of nano-cobaltic-cobaltous oxide and concentration thereof are on its impact as analogue enztme.
The cobalt nitrate salt solusion of 20mL 1mol/L is dropwise instilled 1mol/L sodium carbonate (Na
2cO
3) (Na in solution
2cO
3amount just make cobalt nitrate precipitate completely), 50 DEG C of reaction 1h, deionized water washing is to neutral, and 100 DEG C of dryings, 300 DEG C of roasting 3h, can obtain nano-cobaltic-cobaltous oxide particle, particle diameter is about 9nm.
Fig. 1 is the XRD figure of the nano-cobaltic-cobaltous oxide that the present invention obtains.
embodiment 2
Nano-cobaltic-cobaltous oxide detects content of hydrogen peroxide as Mimetic Peroxidase.
50ml is added, the cobaltosic oxide solution of 1mg/ml, 20ml(5mg/ml in solution) TMB solution, then add the H of different amount
2o
2, make H
2o
2final concentration be 0,10
-9, 5 × 10
-9, 10
-8, 5 × 10
-8, 10
-7, 5 × 10
-7, 10
-6, 5 × 10
-6, 10
-5, 5 × 10
-5, 10
-4, 5 × 10
-4, 10
-3, 5 × 10
-3, 10
-2(mol/L).Add sodium acetate buffer solution (pH=7.0), make the final volume of solution to be measured be 200ml.Microplate reader is used to measure the light absorption value of solution.Simultaneously according to the size of light absorption value, the concentration of hydrogen peroxide can be inferred.
Fig. 2 is take TMB as developer, and nano-cobaltic-cobaltous oxide is detected hydrogen oxide figure.
embodiment 3;
Nano-cobaltic-cobaltous oxide detects content of hydrogen peroxide as Mimetic Peroxidase
50ml is added, the cobaltosic oxide solution of 1mg/ml, 20ml(5mg/ml in solution) ABTS solution, then add the H of different amount
2o
2, make H
2o
2final concentration be 0,10
-9, 5 × 10
-9, 10
-8, 5 × 10
-8, 10
-7, 5 × 10
-7, 10
-6, 5 × 10
-6, 10
-5, 5 × 10
-5, 10
-4, 5 × 10
-4, 10
-3, 5 × 10
-3, 10
-2(mol/L).Add sodium acetate buffer solution (pH=4.0), make the final volume of solution to be measured be 200ml.Microplate reader is used to measure the light absorption value of solution.Simultaneously according to the size of light absorption value, the concentration of hydrogen peroxide can be inferred.
Fig. 3 is take ABTS as developer, and nano-cobaltic-cobaltous oxide is detected hydrogen oxide figure.
Claims (6)
1. a nano-cobaltic-cobaltous oxide measures the application of concentration of hydrogen peroxide as Mimetic Peroxidase, it is characterized in that: nano-cobaltic-cobaltous oxide is as class Mimetic Peroxidase, there is the activity of class peroxidase, its particle diameter is not limited to 9nm, also comprises the nano-cobaltic-cobaltous oxide particle of other sizes.
2. nano-cobaltic-cobaltous oxide according to claim 1 measures the application of concentration of hydrogen peroxide as Mimetic Peroxidase, it is characterized in that, can detect H
2o
2the method of content, detects and is limited to 10
-5.
3. nano-cobaltic-cobaltous oxide according to claim 1 measures the application of concentration of hydrogen peroxide as Mimetic Peroxidase, it is characterized in that, catalyzing hydrogen peroxide oxidation TMB hydrochloride (TMB), becomes blueness from colourless.
4. nano-cobaltic-cobaltous oxide according to claim 1 measures the application of concentration of hydrogen peroxide as Mimetic Peroxidase, it is characterized in that, catalyzing hydrogen peroxide oxidation 2,2 '-hydrazine-bis-(3-ethyl benzo match azoles quinoline-6-sulfonic acid)-diamine salts (ABTS), becomes green from colourless.
5. nano-cobaltic-cobaltous oxide according to claim 1 measures the application of concentration of hydrogen peroxide as Mimetic Peroxidase, and it is characterized in that, its buffer solution is sodium acetate buffer solution.
6. nano-cobaltic-cobaltous oxide according to claim 1 measures the application of concentration of hydrogen peroxide as Mimetic Peroxidase, it is characterized in that, nano-cobaltic-cobaltous oxide has under existing without hydrogen peroxide, still can the ability of phenol substance catalytic, except being excellent Mimetic Peroxidase, or the excellent catalyst of wastewater treatment.
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105044093A (en) * | 2015-06-26 | 2015-11-11 | 上海纳米技术及应用国家工程研究中心有限公司 | Application of nanometer MnOx and CrOx as peroxide mimic enzymes to hydrogen peroxide detection |
| CN105388145A (en) * | 2015-10-16 | 2016-03-09 | 上海纳米技术及应用国家工程研究中心有限公司 | Method for catalyzing TMB and ABTS with nanocrystalline metal oxides serving as oxidation mimic enzyme |
| CN105413750A (en) * | 2015-12-11 | 2016-03-23 | 安徽师范大学 | Preparation method of mimic enzyme |
| CN106315689A (en) * | 2016-07-29 | 2017-01-11 | 兰州大学 | Preparation method and application of graphene oxide@cobaltosic oxide nanocomposite |
| CN106525827A (en) * | 2016-10-20 | 2017-03-22 | 南京东纳生物科技有限公司 | An EGFR immunohistochemical detection kit based on Fe<3>O4 nanoprobes and a using method thereof |
| CN107478832A (en) * | 2017-08-02 | 2017-12-15 | 青岛大学 | One kind imitates enzyme nano material and its application based on phage display polypeptide ligand and magnetic |
| CN107941882A (en) * | 2017-11-27 | 2018-04-20 | 海南师范大学 | A kind of preparation and application research of electrochemica biological sensor part based on cobaltosic oxide and horseradish peroxidase modified electrode |
| CN108645805A (en) * | 2018-05-02 | 2018-10-12 | 天津科技大学 | A kind of new method for exempting to mark quickly detection cysteine |
| CN108998022A (en) * | 2018-08-29 | 2018-12-14 | 江苏科技大学 | A kind of tin-antiomony oxide nano particle and its preparation method and application with peroxidase characteristic |
| CN109499582A (en) * | 2018-11-27 | 2019-03-22 | 中国科学院海洋研究所 | A kind of composite oxides analogue enztme material and its preparation method and application |
| CN110389125A (en) * | 2019-07-22 | 2019-10-29 | 青岛科技大学 | Application of the nano copper selenide as Mimetic Peroxidase detection hydrogen peroxide and L-cysteine |
| CN110479241A (en) * | 2019-08-02 | 2019-11-22 | 电子科技大学 | Improve the active processing method of peroxidase and product of nano enzyme |
| CN111060501A (en) * | 2019-12-06 | 2020-04-24 | 山东理工大学 | Nano cerium peroxide catalyst and method for catalyzing TMB color development by using same |
| CN112717934A (en) * | 2020-11-23 | 2021-04-30 | 淮阴师范学院 | Attapulgite-loaded transition metal oxide composite material and preparation method and application thereof |
| CN113029988A (en) * | 2021-04-14 | 2021-06-25 | 贵阳海关综合技术中心(贵州国际旅行卫生保健中心、贵阳海关口岸门诊部) | Based on inhibiting porous Co3O4Method for detecting multiple heavy metals with nano-disc catalytic activity |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101037676A (en) * | 2006-03-13 | 2007-09-19 | 中国科学院生物物理研究所 | New function and usage of magnetic nano material |
| CN102583582A (en) * | 2012-03-06 | 2012-07-18 | 南京大学 | Co3O4 nanocrystal, preparation method thereof, use thereof and non-enzymatic hydrogen peroxide sensor thereof |
-
2015
- 2015-01-05 CN CN201510001844.2A patent/CN104568934A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101037676A (en) * | 2006-03-13 | 2007-09-19 | 中国科学院生物物理研究所 | New function and usage of magnetic nano material |
| CN102583582A (en) * | 2012-03-06 | 2012-07-18 | 南京大学 | Co3O4 nanocrystal, preparation method thereof, use thereof and non-enzymatic hydrogen peroxide sensor thereof |
Non-Patent Citations (4)
| Title |
|---|
| M.GRUTTADAURIA ET AL.: "Oxidative degradation properties of Co-based catalysts in the presence of ozone", 《APPLIED CATALYSTS B:ENVIRONMENTAL》 * |
| YUMING DONG ET AL.: "《A facile route to controlled synthesis of CO3O4 nanoparticles and their environmental catalytic properties》", 《NANOTECHNOLOGY》 * |
| YUMING DONG: "《catalytic Ozonation of phenol in aqueous solution by Co3O4 nanoparticles》", 《BULL.KOREAN CHEM.SOC》 * |
| 苏莉: "新型过氧化物模拟酶纳米材料在可视化检测葡萄糖中的应用", 《中国博士学位论文全文数据库工程科技Ⅰ辑》 * |
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| CN105388145A (en) * | 2015-10-16 | 2016-03-09 | 上海纳米技术及应用国家工程研究中心有限公司 | Method for catalyzing TMB and ABTS with nanocrystalline metal oxides serving as oxidation mimic enzyme |
| CN105413750A (en) * | 2015-12-11 | 2016-03-23 | 安徽师范大学 | Preparation method of mimic enzyme |
| CN106315689A (en) * | 2016-07-29 | 2017-01-11 | 兰州大学 | Preparation method and application of graphene oxide@cobaltosic oxide nanocomposite |
| CN106525827B (en) * | 2016-10-20 | 2019-04-09 | 南京东纳生物科技有限公司 | One kind being based on Fe3O4The EGFR immunologic combined detection reagent kit and its application method of nano-probe |
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| CN109499582B (en) * | 2018-11-27 | 2021-09-28 | 中国科学院海洋研究所 | Composite oxide mimic enzyme material and preparation method and application thereof |
| CN110389125A (en) * | 2019-07-22 | 2019-10-29 | 青岛科技大学 | Application of the nano copper selenide as Mimetic Peroxidase detection hydrogen peroxide and L-cysteine |
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| CN111060501A (en) * | 2019-12-06 | 2020-04-24 | 山东理工大学 | Nano cerium peroxide catalyst and method for catalyzing TMB color development by using same |
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| CN113029988A (en) * | 2021-04-14 | 2021-06-25 | 贵阳海关综合技术中心(贵州国际旅行卫生保健中心、贵阳海关口岸门诊部) | Based on inhibiting porous Co3O4Method for detecting multiple heavy metals with nano-disc catalytic activity |
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