CN104568675A - DGT testing analysis method for phosphorus absorption characteristics of lake aquatic plant root zone - Google Patents
DGT testing analysis method for phosphorus absorption characteristics of lake aquatic plant root zone Download PDFInfo
- Publication number
- CN104568675A CN104568675A CN201510009357.0A CN201510009357A CN104568675A CN 104568675 A CN104568675 A CN 104568675A CN 201510009357 A CN201510009357 A CN 201510009357A CN 104568675 A CN104568675 A CN 104568675A
- Authority
- CN
- China
- Prior art keywords
- phosphorus
- concentration
- dgt
- aquatic plant
- sediment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides a DGT testing analysis method for phosphorus absorption characteristics of a lake aquatic plant root zone. The method is a field determination and analysis method in the aquatic plant root zone based on DGT, and an analysis method for parameters, and sediment pore water/sediment solid phase and plant samples. Content determination is carried out with respect to a field aquatic plant-water-sediment system; a series of parameters (CDGT, Rdiff and CE) representing solid-liquid phase distribution, equilibrium concentration, sediment phosphorus storage warehouse volume and DGT are obtained; mathematical statistics and operation of plant absorption parameters are carried out according to the parameters; and prediction and evaluation of an absorption mechanism and characteristics of the aquatic plant root zone are carried out.
Description
Technical field
The invention belongs to environmental science and field of earth sciences, be specifically related to the DGT method for testing and analyzing of a kind of lake aquatic plant roots district P elements Absorption Characteristics.
Technical background
The eutrophication in lake pollution especially lake is the subject matter that China's Study of Water Environment faces for a long time.Lake eutrophication often shows as aquatic vegetation decline and even withers away, water quality deterioration.Restoration and reconstruction aquatic vegetation, particularly restoration and reconstruction hydrophyte vegetation are the important measures of administering nutrition enriched shallow lake.But the fundamental research of hydrophyte vegetation repair technology relatively lags behind.The understanding of hydrophyte rhizodeposition thing phosphorus transfer and Forms Transformation and seasonal variety thereof is the key scientific problems that must solve in restoration and reconstruction submerged vegetation, be the key issue being badly in need of in nutrition enriched shallow lake restoration of the ecosystem answering, be also the content disclosing the research of shallow lake eutrophication genesis mechanism needs further simultaneously.Rhizosphere is the region that hydrophyte enlivens the most on sediment phosphorus migration and Forms Transformation impact, to disclose the migration of hydrophyte on the absorption of sediment phosphorus and the impact of Forms Transformation from mechanism, must conduct a research for the rhizosphere of hydrophyte.
In prior art, typically use chemical leaching test and the phosphorus in soil or sediment is measured, but chemical leaching test is the real response of reactivity based on chemical reagent instead of element validity.At present, in plant roots district, test is mainly used in the prediction of plant root to metallic element mechanism of absorption and the research of absorption mechanism in soil to DGT (diffusive gradients in thin-films technology), but the sediments zones that lake aquatic plant grows never is carried out to the research of the mechanism of DGT test and the absorption phosphorus of plant root based on this or other element.Therefore, how according to lake eutrophication characteristic sum hydrophyte root feature, correctly carry out the DGT test in lake sediment hydrophyte root district, select pre-service and the assay method of suitable sediment/plant sample, and carry out root absorption feature in conjunction with the phosphorus yield result of DGT/ sediment/plant, predict the research of the DGT indicator feature of the phosphorus content of plant root absorption and plant, be the still unsolved difficult problems of those skilled in the art.
Summary of the invention
What the present invention solved is lack the P elements Absorption Characteristics analytical approach being applicable to lake eutrophication characteristic sum hydrophyte root feature in prior art, and then provides the DGT testing and analysis method of a kind of lake aquatic plant roots district P elements Absorption Characteristics.
The technical scheme that the present invention realizes above-mentioned purpose is:
A DGT method for testing and analyzing for lake aquatic plant roots district P elements Absorption Characteristics, comprises the steps:
(1) select multiple sampling point in the root district of lake aquatic plant, multiple circular DGT of the hydrated ferric oxide driving oxygen process that completes is put into the root district sediment of described multiple sampling point respectively and keeps at least 24 hours;
(2) circular for multiple described hydrated ferric oxide DGT is taken out, with deionized water rinsing totally and the hydrated ferric oxide fixing glue of taking out in the circular DGT of described hydrated ferric oxide; Respectively standing wash-out is carried out to each described hydrated ferric oxide fixing glue with acid solution, utilize the concentration of active phosphorus in each eluent of the anti-spectrphotometric method for measuring of molybdenum antimony, finally calculate the DGT concentration C of each sampling point according to the concentration of active phosphorus in each eluent respectively
dGT;
(3) the lake aquatic plant of described multiple sampling point and the sediment in described lake aquatic plant roots district is taken out respectively with grab type sampling thief; Two parts of sediment samples are gathered in the sediment in the described lake aquatic plant roots district of each sampling point, sediment sample described in a copy of it is carried out centrifugal treating under inert gas conditions, collect supernatant, adopt the concentration of active phosphorus in supernatant described in the anti-spectrphotometric method for measuring of molybdenum antimony, namely obtain the concentration C of active phosphorus in sediment interstitial water
solu;
Carry out drying to another part of sediment sample and obtain sedimentary particle, first working concentration is the NH of 1mol/L successively
4cl aqueous solution, BD reagent solution, the HCl aqueous solution of concentration to be the NaOH aqueous solution of 0.1mol/L and concentration be 0.5mol/L is extracted described sedimentary particle at ambient temperature as extraction agent, finally re-using concentration is that the NaOH aqueous solution of 0.1mol/L is extracted described sedimentary particle under the temperature conditions of 85 DEG C, carry out centrifugal after extracting balance each time, the concentration of the soluble activating state phosphorus in centrifuged supernatant is measured by molybdenum blue method, thus obtain weak binding state phosphorus successively respectively, isotope of redox-sensitive state phosphorus, hydrous metal oxides bound phosphorus, the concentration of calcium bound phosphorus and inertia organic phosphorus, sedimental phosphorus reservoir Cs is the concentration sum of described weak binding state phosphorus and described isotope of redox-sensitive state phosphorus, wherein said BD reagent solution is NaHCO
3and Na
2s
2o
4mixed aqueous solution, described NaHCO
3and Na
2s
2o
4concentration be 0.11mol/L,
(4) the sedimental particle density in the described lake aquatic plant roots district of each sampling point and particle voidage are measured, utilize described particle voidage to calculate the coefficient of diffusion of sediment interstitial water;
(5) spread in the coefficient of diffusion of solid-liquid partition factor and response time and described sedimental particle density, diffusion layer, the thickness of diffusion layer, the coefficient of diffusion of described sediment interstitial water, the circular DGT of described hydrated ferric oxide in the initial concentration input DIFS software of the voidage of glue, described sedimental particle voidage, described sediment interstitial water, calculate R
diffvalue, the value of wherein said solid-liquid partition factor is set as 10
-10cm
3g
-1, the value of described response time is set as 10
10s; Then following formulae discovery is utilized to obtain the effective concentration C of the phosphorus of described multiple sampling point respectively
e:
C
E＝C
DGT/R
diff；
(6) concentration of phosphorus in the root of the described lake aquatic plant of each sampling point, stem, leaf is measured respectively;
(7) by the DGT concentration C of multiple described sampling point
dGT, described sedimental weak binding state phosphorus concentration, isotope of redox-sensitive state phosphorus concentration, hydrous metal oxides bound phosphorus concentration, calcium bound phosphorus concentration and inertia organic phosphorus concentration, sedimental phosphorus reservoir Cs, phosphorus effective concentration C
e, the concentration C of active phosphorus in sediment interstitial water
solucarry out mathematical statistics with the concentration of phosphorus in the root of the lake aquatic plant of described multiple sampling point, stem, leaf respectively, obtain the phosphorus Absorption Characteristics in hydrophyte root district.
Lake aquatic plant described in step (1) is hay grass.
The depth of water in the region at described lake aquatic plant place is 1.2-1.5m; The height of the aerial part of described lake aquatic plant is 40-50cm.
Each described sampling point at least grows lake aquatic plant described in three strains, and described in three strains, the root system of lake aquatic plant is entwined together.
To the method that the circular DGT of described hydrated ferric oxide drives oxygen process be: circular for described hydrated ferric oxide DGT is put into the plastic containers that the pure NaCl solution of 0.01M top grade is housed, passes into high pure nitrogen and keep 18 hours.
In step (2), the sulfuric acid of 0.25M is adopted to carry out standing wash-out to described hydrated ferric oxide fixing glue; The time of described standing wash-out is 24 hours.
Described in step (4), the assay method of particle density and particle voidage is: getting weight is W
0the sediment of described post sample, after drying at 105 DEG C, the weight of again weighing is W
1, be then calculated as follows described particle density ρ
cwith particle voidage Φ s:
ρ
C＝W
1/(W
0-W
1)/ρ
W)
Φ
s＝dp/(ρ
C+d
p)
Wherein, ρ
wthe density of water; Dp is 2.65g/cm
3.
The method measuring the concentration of phosphorus in the root of the described lake aquatic plant of each point sample, stem, leaf in step (6) is respectively:
A () uses deionized water to clean described lake aquatic plant, and gather the sample of the root of the described lake aquatic plant of each sampling point, stem and leaf respectively;
(b) by described, the sample drying of stem and leaf to constant weight, and takes described, the sample dry weight of stem and leaf respectively;
(c) respectively by described, the sample of stem and leaf is positioned in microwave eliminating device, add salpeter solution and clear up; After clearing up, utilize molybdenum blue method to determine phosphorus content in digestion solution, described of unit mass, the content of stem and phosphorus corresponding to leaf can be calculated respectively.
The method of carrying out mathematical statistics in step (7) is linear regression method.
DIFS software described in the present invention refers to that sediment DGT induces diffusion flux software.
The analytical approach of lake aquatic plant roots district of the present invention P elements Absorption Characteristics, its principle is mainly based on DGT analytical approach and parameter, the sediment interstitial water/analytical approach of sediment solid phase and the analytical approach of plant sample, for the mensuration of the foregoing that on-the-spot hydrophyte-water-sediment carries out, obtain a series of expression solid liquid phase distribution, equilibrium concentration, sediment reservoir capacity and DGT parameter (C
dGT, R
diffand C
e), carry out the computing of mathematical statistics and plant absorption parameter according to above parameter, carry out the Forecast and evaluation of plant absorption mechanism and feature.DGT carries out the absorption of Elements In Sediment as plant root, and the test local of DGT technology in sediment reduces concentration of element in sediment interstitial water, and resupplies effect in response to solution and solid phase reservoir.The flux that DGT tests in sediment can draw effective concentration C
e.DGT surface mean concentration C
diffwith the concentration C of active phosphorus in sediment interstitial water
soluratio can be expressed as:
R
diff＝C
diff/C
solu
Wherein, under the DGT test condition of " being only diffusion ", C
diffdGT concentration in this case, C
soluit is active phosphorus concentration in the root district sediment interstitial water of conventional method mensuration; R
diffbe in this case resupply parameter.R
diffdetermined by the size of equipment, running time and sediment physicochemical property.It can use the calculated with mathematical model of diffusion equation.In normal circumstances, C
soludo not represent effective concentration (C
e), C
ethe concentration that a DGT equipment or plant root experience.Effective concentration C
ethe concentration comprising sediment interstitial water and the concentration obtained from solid phase, effective concentration C
ecan obtain with following formula:
C
E＝C
DGT/R
diff
So, DGT provides effective concentration C
edirect mensuration.This effective concentration C
eit is the true reflection to being measured by the DGT diffusion process similar to plant absorption mechanism.Therefore, the effective concentration C in the present invention
ejust provide the evaluating of the absorption active phosphorus feature for root in the sediment produced by spreading.Thus can according to DGT test result (C
dGTand C
e) and sediment, hydrophyte and Interstitial Water in the measurement result of P elements, carry out mathematical statistics (mainly linear regression method), obtain phosphorus total amount and sediment/Interstitial Water phosphorus/active phosphorus in hydrophyte root/stem/leaf, the extraction phase of sedimental phosphorus, DGT concentration and C
equantitative relation between value (effective concentration) and phosphorus, at the transportion and transformation of hydrophyte root district absorption process, especially can carry out the research of DGT to the forecast function that hydrophyte root absorption and phosphorus distribute in plant.
Accompanying drawing explanation
The phosphorus concentration C in hay grass roots portion shown in Fig. 1
rootwith effective concentration C
ebetween linear relationship chart;
The phosphorus concentration C in hay grass roots portion shown in Fig. 2
rootwith C
dGTbetween linear relationship chart;
The phosphorus concentration C in hay grass roots portion shown in Fig. 3
rootwith the concentration C of active phosphorus in sediment interstitial water
solubetween linear relationship chart;
The phosphorus concentration C in hay grass roots portion shown in Fig. 4
rootand the linear relationship chart between sedimental phosphorus reservoir Cs;
The phosphorus concentration C in hay grass blade portion shown in Fig. 5
stemwith effective concentration C
ebetween linear relationship chart;
The phosphorus concentration C in hay grass blade portion shown in Fig. 6
stemwith C
dGTbetween linear relationship chart;
The phosphorus concentration C in hay grass blade portion shown in Fig. 7
stemwith the concentration C of active phosphorus in sediment interstitial water
solubetween linear relationship chart;
The phosphorus concentration C in hay grass blade portion shown in Fig. 8
stemand the linear relationship chart between sedimental phosphorus reservoir Cs;
The phosphorus concentration C of hay blade of grass shown in Fig. 9
leafwith effective concentration C
ebetween linear relationship chart;
The phosphorus concentration C of hay blade of grass shown in Figure 10
leafwith C
dGTbetween linear relationship chart;
The phosphorus concentration C of hay blade of grass shown in Figure 11
leafwith the concentration C of active phosphorus in sediment interstitial water
solubetween linear relationship chart;
The phosphorus concentration C of hay blade of grass shown in Figure 12
leafand the linear relationship chart between sedimental phosphorus reservoir Cs.
Embodiment
The DGT method for testing and analyzing of the lake aquatic plant roots district P elements Absorption Characteristics described in the present embodiment, comprises the steps:
(1) select hydrophyte survey region at Lake Erhai, the mean depth in described region is 1.2 ~ 1.5m, has a kind of hydrophyte-hay grass in the single growth in this region; Select 10 sampling points in the hydrophyte root district in this region, each sampling point is distributed with 3 hay grass, and the well developed root system of described 3 hay grass, main root is longer.The height of every careless part on the ground of described hay is all between 40 ~ 50cm; Wherein, described hydrophyte root district refers on the sediment of aquatic plants growth, with every hydrophyte for the center of circle, diameter be 4cm deposit surface within the scope of and the degree of depth is the region within the scope of 3cm.
Take out the circular DGT of 10 hydrated ferric oxides and carry out driving oxygen process, the method of driving oxygen process described in carrying out is: circular for described 10 hydrated ferric oxides DGT is individually put into the plastic containers that the pure NaCl solution of 0.01M top grade is housed, in described NaCl solution, pass into high pure nitrogen and keep 18 hours, namely completing and drive oxygen process; 10 are completed the circular DGT of the hydrated ferric oxide driving oxygen process to be placed on respectively in the root district sediment of described 10 sampling points and to keep 24 hours; The circular DGT of the hydrated ferric oxide used in the present embodiment is by DGT Research Ltd. (Lancaster University) Company, and model is Ferrihyrite-DGT.
(2) circular for 10 described hydrated ferric oxides DGT are taken out, with deionized water rinsing totally and the hydrated ferric oxide fixing glue of taking out in the circular DGT of described hydrated ferric oxide; Adopt the sulfuric acid of 0.25M to carry out standing wash-out 24 hours to each described hydrated ferric oxide fixing glue respectively, utilize the concentration C of active phosphorus in each eluent of the anti-spectrphotometric method for measuring of molybdenum antimony
e, finally calculate the DGT concentration C of each sampling point respectively according to the following formula
dGT:
C
DGT＝MΔg/DAt
Wherein, t is the running time; A is the exposed area of described hydrated ferric oxide fixing glue; D is the coefficient of diffusion spreading peptization matter in the circular DGT of described hydrated ferric oxide; Δ g is the thickness of diffusion layer of the circular DGT of hydrated ferric oxide; M is the quality of the solute absorbed, and the computing formula of M is as follows:
M＝C
e(V
gel+V
elution)/f
e
Wherein, C
ethe concentration of active phosphorus in eluent, V
gelthe volume of described hydrated ferric oxide fixing glue, V
elutionthe volume of described eluent, f
ebe wash-out coefficient, value is 1.0; C
dGTresult of calculation as shown in table 2.
(3) the lake aquatic plant of described 10 sampling points and the sediment in described lake aquatic plant roots district is taken out respectively with grab type sampling thief; Two parts of sediment samples are gathered in the sediment in the described lake aquatic plant roots district of each sampling point, sediment sample described in a copy of it is carried out centrifugal treating under inert gas conditions, collect supernatant, adopt the concentration of active phosphorus in supernatant described in the anti-spectrphotometric method for measuring of molybdenum antimony, namely obtain the concentration C of active phosphorus in sediment interstitial water
solu;
Carry out drying to another part of sediment sample and obtain sedimentary particle, first working concentration is the NH of 1mol/L successively
4cl aqueous solution, BD reagent solution, the HCl aqueous solution of concentration to be the NaOH aqueous solution of 0.1mol/L and concentration be 0.5mol/L is extracted described sedimentary particle at ambient temperature as extraction agent, finally re-using concentration is that the NaOH aqueous solution of 0.1mol/L is extracted described sedimentary particle under the temperature conditions of 85 DEG C, carry out centrifugal after extracting balance each time, the concentration of the soluble activating state phosphorus in centrifuged supernatant is measured by molybdenum blue method, thus obtain weak binding state phosphorus successively respectively, isotope of redox-sensitive state phosphorus, hydrous metal oxides bound phosphorus, the concentration of calcium bound phosphorus and inertia organic phosphorus, sedimental phosphorus reservoir Cs is the concentration sum of described weak binding state phosphorus and described isotope of redox-sensitive state phosphorus, wherein said BD reagent solution is NaHCO
3and Na
2s
2o
4mixed aqueous solution, described NaHCO
3and Na
2s
2o
4concentration be 0.11mol/L, the present embodiment adopts the standard substance GSS2 (purchased from Chinese Geophysical Ano Geochemical Exploration research institute) of sediment phosphorus to carry out the mensuration of the phosphorus bank recovery, and its recovery is 95.9%.
(4) the sedimental particle density in the described lake aquatic plant roots district of each sampling point and particle voidage are measured, concrete grammar is: get 50g sediment, after drying at 105 DEG C, again weigh, be then calculated as follows described particle density ρ
cwith particle voidage Φ
s:
ρ
C＝W
1/(W
0-W
1)/ρ
W)
Φ
s＝dp/(ρ
C+d
p)
Wherein, ρ
wthe density of water; Dp is 2.65g/cm
3, W
0for 50g; W
1for the weight of again weighing after oven dry;
Utilize described particle voidage Φ
scalculate the diffusion coefficient D s of sediment interstitial water, its computing formula is as follows:
Ds＝D
0/(1-2lnΦ
s)
Wherein, D
0be the coefficient of diffusion of active phosphorus in water, Φ s is the voidage of particle.
(5) by solid-liquid partition factor K
dwith response time T
cand described sedimental particle density ρ
c, diffusion layer diffusion coefficient D
d, diffusion layer thickness d
g, described sediment interstitial water diffusion coefficient D
s, spread the voidage pordif of glue, described sedimental particle voidage Φ in the circular DGT of described hydrated ferric oxide
s, the concentration C of active phosphorus in described sediment interstitial water
soluin input DIFS software, calculate R
diffvalue, wherein said solid-liquid partition factor K
dsetting value be 10
-10cm
3g
-1, the setting value of described response time Tc is 10
10s;
The voidage pordif of the described diffusion glue of the circular DGT of the described hydrated ferric oxide used in the present embodiment is 0.95; The input and output parameter of DIFS described in the present embodiment is as shown in table 1; Then following formulae discovery is utilized to obtain the effective concentration C of the phosphorus of described multiple sampling point respectively
e:
C
E＝C
DGT/R
diff；
Cs and C calculated in the present embodiment
ebe worth as shown in table 2.
(6) use deionized water to clean described lake aquatic plant, measure the concentration of phosphorus in the root of the described lake aquatic plant of each sampling point, stem, leaf respectively; Concrete assay method is:
A () uses deionized water to clean described lake aquatic plant, and gather the sample of the root of the described lake aquatic plant of each sampling point, stem and leaf respectively;
(b) by described, the sample of stem and leaf respectively under 105 DEG C of conditions after dry 1 hour, more lower dry 72 little of a constant weight under 80 DEG C of conditions, and take described, the sample dry weight of stem and leaf respectively;
(c) respectively by described of 0.25g, the sample of stem and leaf is positioned in microwave eliminating device, adds 5mL red fuming nitric acid (RFNA) and 5mL water is cleared up; After clearing up, utilize molybdenum blue method to determine phosphorus content in digestion solution, by phosphorus content divided by 0.25g, described of unit mass, the content of stem and phosphorus corresponding to leaf can be calculated respectively, the phosphorus concentration C namely in root, stem and leaf
root, C
stemand C
leaf.
In order to verify the accuracy to phosphorus concentration test in root, stem, leaf in this step, the present embodiment also carries out the mensuration of standard substance simultaneously, plant standard sample used is: GBW07603 and GBW08504, and the recovery that these two kinds of samples record is respectively 90.48% and 105.8%.
(7) by the DGT concentration C of described 10 sampling points
dGT, sedimental weak binding state phosphorus concentration, isotope of redox-sensitive state phosphorus concentration, hydrous metal oxides bound phosphorus concentration, calcium bound phosphorus concentration and inertia organic phosphorus concentration, sedimental phosphorus reservoir Cs, phosphorus effective concentration C
e, the concentration C of active phosphorus in sediment interstitial water
solumathematical statistics is carried out respectively with the concentration of phosphorus in the root of the lake aquatic plant of described 10 sampling points, stem, leaf; Result shows, DGT concentration C
dGT, effective concentration C
e, in phosphorus bank Cs and hay grass roots/stem/leaf phosphorus concentration between linear relationship better, especially C
dGT, C
ebest with the concentration dependence of phosphorus in hay grass roots/stem/leaf.The C that the present embodiment obtains
dGT, C
solu, C
e, Cs and C
root, C
stemand C
leafbetween linear relationship as shown in figs. 1-12; This fully indicates DGT concentration especially C
econcentration for root district phosphorus effective ingredient and because the effect that resupplies of particle that root absorption causes has indicator effect.And in the front two-phase of Phosphorus in Sediments and hay grass roots/stem/leaf, the linear relationship of phosphorus indicates hay grass roots portion dominant absorption is weak binding state phosphorus (mainly exchangeable phosphate) in sediment and isotope of redox-sensitive state phosphorus (mainly Fe-P).
The DGT test of Erhai hydrophyte-Jiao Caogenqu is shown, DGT can complete the test of lake aquatic plant roots district preferably, the mechanism of simulation root absorption phosphorus, and the distribution in root absorption and plant is predicted, complete the research at the Transport And Transformation of sediment/Interstitial Water/root/stem/leaf of lake aquatic plant root mechanism of absorption and phosphorus.Further, except carrying out except the test of root district for the hydrophyte of growth in lake in the present embodiment, the method for testing and analyzing in the present invention is also applicable to carry out root district testing and analysis to the lake aquatic plant of laboratory culture.
The input and output parameter of table 1 DIFS
The C that table 2 the present embodiment calculates
dGT, C
soluand C
evalue:
| Sampling point is numbered | C E(nmol/ml) | C DGT(nmolml -1) | Cs(μmol/g) |
| 1 | 51.61 | 4.8 | 1.41 |
| 2 | 66.74 | 6.14 | 1.66 |
| 3 | 84.32 | 6.24 | 1.89 |
| 4 | 115.70 | 10.76 | 2.05 |
| 5 | 213.46 | 16.65 | 2.20 |
| 6 | 219.26 | 20.83 | 2.33 |
| 7 | 295.27 | 26.87 | 2.36 |
| 8 | 312.82 | 24.4 | 2.48 |
| 9 | 372.11 | 28.28 | 2.80 |
| 10 | 500.00 | 31.5 | 3.27 |
Obviously, above-described embodiment is only for clearly example being described, and the restriction not to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.And thus the apparent change of extending out or variation be still among the protection domain of the invention.
Claims (9)
1. a DGT method for testing and analyzing for lake aquatic plant roots district P elements Absorption Characteristics, comprises the steps:
(1) select multiple sampling point in the root district of lake aquatic plant, multiple circular DGT of the hydrated ferric oxide driving oxygen process that completes is put into the root district sediment of described multiple sampling point respectively and keeps at least 24 hours;
(2) circular for multiple described hydrated ferric oxide DGT is taken out, with deionized water rinsing totally and the hydrated ferric oxide fixing glue of taking out in the circular DGT of described hydrated ferric oxide; Respectively standing wash-out is carried out to each described hydrated ferric oxide fixing glue with acid solution, utilize the concentration of active phosphorus in each eluent of the anti-spectrphotometric method for measuring of molybdenum antimony, finally calculate the DGT concentration C of each sampling point according to the concentration of active phosphorus in each eluent respectively
dGT;
(3) the lake aquatic plant of described multiple sampling point and the sediment in described lake aquatic plant roots district is taken out respectively with grab type sampling thief; Two parts of sediment samples are gathered in the sediment in the described lake aquatic plant roots district of each sampling point, sediment sample described in a copy of it is carried out centrifugal treating under inert gas conditions, collect supernatant, adopt the concentration of active phosphorus in supernatant described in the anti-spectrphotometric method for measuring of molybdenum antimony, namely obtain the concentration C of active phosphorus in sediment interstitial water
solu;
Carry out drying to another part of sediment sample and obtain sedimentary particle, first working concentration is the NH of 1mol/L successively
4cl aqueous solution, BD reagent solution, the HCl aqueous solution of concentration to be the NaOH aqueous solution of 0.1mol/L and concentration be 0.5mol/L is extracted described sedimentary particle at ambient temperature as extraction agent, finally re-using concentration is that the NaOH aqueous solution of 0.1mol/L is extracted described sedimentary particle under the temperature conditions of 85 DEG C, carry out centrifugal after extracting balance each time, the concentration of the soluble activating state phosphorus in centrifuged supernatant is measured by molybdenum blue method, thus obtain weak binding state phosphorus successively respectively, isotope of redox-sensitive state phosphorus, hydrous metal oxides bound phosphorus, the concentration of calcium bound phosphorus and inertia organic phosphorus, sedimental phosphorus reservoir Cs is the concentration sum of described weak binding state phosphorus and described isotope of redox-sensitive state phosphorus, wherein said BD reagent solution is NaHCO
3and Na
2s
2o
4mixed aqueous solution, described NaHCO
3and Na
2s
2o
4concentration be 0.11mol/L,
(4) the sedimental particle density in the described lake aquatic plant roots district of each sampling point and particle voidage are measured, utilize described particle voidage to calculate the coefficient of diffusion of sediment interstitial water;
(5) spread in the coefficient of diffusion of solid-liquid partition factor and response time and described sedimental particle density, diffusion layer, the thickness of diffusion layer, the coefficient of diffusion of described sediment interstitial water, the circular DGT of described hydrated ferric oxide in the initial concentration input DIFS software of the voidage of glue, described sedimental particle voidage, described sediment interstitial water, calculate R
diffvalue, the value of wherein said solid-liquid partition factor is set as 10
-10cm
3g
-1, the value of described response time is set as 10
10s; Then following formulae discovery is utilized to obtain the effective concentration C of the phosphorus of described multiple sampling point respectively
e:
C
E＝C
DGT/R
diff；
(6) concentration of phosphorus in the root of the described lake aquatic plant of each sampling point, stem, leaf is measured respectively;
(7) by the DGT concentration C of multiple described sampling point
dGT, described sedimental weak binding state phosphorus concentration, isotope of redox-sensitive state phosphorus concentration, hydrous metal oxides bound phosphorus concentration, calcium bound phosphorus concentration and inertia organic phosphorus concentration, sedimental phosphorus reservoir Cs, phosphorus effective concentration C
e, the concentration C of active phosphorus in sediment interstitial water
solucarry out mathematical statistics with the concentration of phosphorus in the root of the lake aquatic plant of described multiple sampling point, stem, leaf respectively, obtain the phosphorus Absorption Characteristics in hydrophyte root district.
2. the DGT method for testing and analyzing of lake aquatic plant roots district according to claim 1 P elements Absorption Characteristics, is characterized in that, lake aquatic plant described in step (1) is hay grass.
3. the DGT method for testing and analyzing of lake aquatic plant roots district according to claim 1 and 2 P elements Absorption Characteristics, is characterized in that, the depth of water in the region at described lake aquatic plant place is 1.2-1.5m; The height of the aerial part of described lake aquatic plant is 40-50cm.
4. according to the DGT method for testing and analyzing of the arbitrary described lake aquatic plant roots district P elements Absorption Characteristics of claim 1-3, it is characterized in that, each described sampling point at least grows lake aquatic plant described in three strains, and described in three strains, the root system of lake aquatic plant is entwined together.
5. according to the DGT method for testing and analyzing of the arbitrary described lake aquatic plant roots district P elements Absorption Characteristics of claim 1-4, it is characterized in that, to the method that the circular DGT of described hydrated ferric oxide drives oxygen process be: circular for described hydrated ferric oxide DGT is put into the plastic containers that the pure NaCl solution of 0.01M top grade is housed, passes into high pure nitrogen and keep 18 hours.
6. according to the DGT method for testing and analyzing of the arbitrary described lake aquatic plant roots district P elements Absorption Characteristics of claim 1-5, it is characterized in that, in step (2), adopt the sulfuric acid of 0.25M to carry out standing wash-out to described hydrated ferric oxide fixing glue; The time of described standing wash-out is 24 hours.
7., according to the DGT method for testing and analyzing of the arbitrary described lake aquatic plant roots district P elements Absorption Characteristics of claim 1-6, it is characterized in that, described in step (4), the assay method of particle density and particle voidage is: getting weight is W
0the sediment of described post sample, after drying at 105 DEG C, the weight of again weighing is W
1, be then calculated as follows described particle density ρ
cwith particle voidage Φ s:
ρ
C＝W
1/(W
0-W
1)/ρw)
Φ
s＝dp/(ρC+d
p)
Wherein, ρ w is the density of water; Dp is 2.65g/cm
3.
8. according to the DGT method for testing and analyzing of the arbitrary described lake aquatic plant roots district P elements Absorption Characteristics of claim 1-7, it is characterized in that, the method measuring the concentration of phosphorus in the root of the described lake aquatic plant of each point sample, stem, leaf in step (6) is respectively:
A () uses deionized water to clean described lake aquatic plant, and gather the sample of the root of the described lake aquatic plant of each sampling point, stem and leaf respectively;
(b) by described, the sample drying of stem and leaf to constant weight, and takes described, the sample dry weight of stem and leaf respectively;
(c) respectively by described, the sample of stem and leaf is positioned in microwave eliminating device, add salpeter solution and clear up; After clearing up, utilize molybdenum blue method to determine phosphorus content in digestion solution, described of unit mass, the content of stem and phosphorus corresponding to leaf can be calculated respectively.
9., according to the DGT method for testing and analyzing of the arbitrary described lake aquatic plant roots district P elements Absorption Characteristics of claim 1-8, it is characterized in that, the method for carrying out mathematical statistics in step (7) is linear regression method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510009357.0A CN104568675B (en) | 2015-01-08 | 2015-01-08 | The DGT method for testing and analyzing of lake aquatic plant roots area P elements Absorption Characteristics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510009357.0A CN104568675B (en) | 2015-01-08 | 2015-01-08 | The DGT method for testing and analyzing of lake aquatic plant roots area P elements Absorption Characteristics |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104568675A true CN104568675A (en) | 2015-04-29 |
| CN104568675B CN104568675B (en) | 2018-01-09 |
Family
ID=53085253
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510009357.0A Active CN104568675B (en) | 2015-01-08 | 2015-01-08 | The DGT method for testing and analyzing of lake aquatic plant roots area P elements Absorption Characteristics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104568675B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106568689A (en) * | 2016-10-17 | 2017-04-19 | 农业部环境保护科研监测所 | Total-dissociation DGT combined film for determination of soil available phosphorus and preparation method thereof |
| CN112818284A (en) * | 2021-02-02 | 2021-05-18 | 中国水利水电科学研究院 | Ecological risk evaluation method for heavy metal pollutants in sediment |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101629881A (en) * | 2009-08-05 | 2010-01-20 | 中国科学院南京地理与湖泊研究所 | Phosphorus fixing film applied to diffusive gradients in thin-films measurement technology |
| CN102507388A (en) * | 2011-10-24 | 2012-06-20 | 河海大学 | Improved phosphorus fixing film used in DGT (diffusive gradients in thin films) measurement and preparation method thereof |
-
2015
- 2015-01-08 CN CN201510009357.0A patent/CN104568675B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101629881A (en) * | 2009-08-05 | 2010-01-20 | 中国科学院南京地理与湖泊研究所 | Phosphorus fixing film applied to diffusive gradients in thin-films measurement technology |
| CN102507388A (en) * | 2011-10-24 | 2012-06-20 | 河海大学 | Improved phosphorus fixing film used in DGT (diffusive gradients in thin films) measurement and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| PHIL MONBET: "Combined Gel Probes for the In Situ Determination of Dissolved Reactive Phosphorus in Porewaters and Characterization of Sediment Reactivity", 《ENVIRON. SCI. TECHNOL.》 * |
| 张丽媛等: "洋河水库流域土壤与库区沉积物中磷形态特征研究", 《中国环境科学》 * |
| 王圣瑞等: "湖泊沉积物中水溶性有机质对吸附磷的影响", 《土壤学报》 * |
| 钱宝等: "湖泊沉积物_水微界面上磷的释放过程研究", 《水利学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106568689A (en) * | 2016-10-17 | 2017-04-19 | 农业部环境保护科研监测所 | Total-dissociation DGT combined film for determination of soil available phosphorus and preparation method thereof |
| CN106568689B (en) * | 2016-10-17 | 2019-06-18 | 农业部环境保护科研监测所 | Measure the release DGT combination film of complete solution and preparation method thereof of soil available phosphorus |
| CN112818284A (en) * | 2021-02-02 | 2021-05-18 | 中国水利水电科学研究院 | Ecological risk evaluation method for heavy metal pollutants in sediment |
| CN112818284B (en) * | 2021-02-02 | 2023-09-05 | 中国水利水电科学研究院 | Ecological risk evaluation method for heavy metal pollutants in sediment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104568675B (en) | 2018-01-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Preti et al. | Root profile assessment by means of hydrological, pedological and above-ground vegetation information for bio-engineering purposes | |
| Grover et al. | The link between peat hydrology and decomposition: Beyond von Post | |
| Rothfuss et al. | Partitioning evapotranspiration fluxes into soil evaporation and plant transpiration using water stable isotopes under controlled conditions | |
| Bonadonna et al. | Total grain-size distribution and volume of tephra-fall deposits | |
| Yoshida et al. | Concentrations of alkali and alkaline earth elements in mushrooms and plants collected in a Japanese pine forest, and their relationship with 137Cs | |
| Franz et al. | Ecosystem‐scale measurements of biomass water using cosmic ray neutrons | |
| Van der Laan et al. | Modelling nitrogen leaching: Are we getting the right answer for the right reason? | |
| Neff et al. | The role of dust storms in total atmospheric particle concentrations at two sites in the western US | |
| Olsson et al. | Determination of weathering rates based on geochemical properties of the soil | |
| Huang et al. | How much information can soil solute profiles reveal about groundwater recharge? | |
| McCleskey et al. | Electrical conductivity method for natural waters | |
| Slessarev et al. | Quantifying the effects of switchgrass (Panicum virgatum) on deep organic C stocks using natural abundance 14C in three marginal soils | |
| Duan et al. | Field capacity in black soil region, northeast China | |
| Yuan et al. | An enhanced MOD16 evapotranspiration model for the Tibetan Plateau during the unfrozen season | |
| Spain et al. | Phosphorus dynamics in a tropical forest soil restored after strip mining | |
| Carbone et al. | Constrained partitioning of autotrophic and heterotrophic respiration reduces model uncertainties of forest ecosystem carbon fluxes but not stocks | |
| CN109829534B (en) | Method, device and system for evaluating community stability of opencast coal mine refuse dump | |
| CN104318123A (en) | Assessment method of contribution of shellfish biology deposition to offshore environment sediment organic carbon | |
| Bobrov et al. | Testate amoebae and environmental features of polygon tundra in the Indigirka lowland (East Siberia) | |
| Yanai et al. | Quantifying uncertainty in forest nutrient budgets | |
| Li et al. | Response of mercury accumulation to anthropogenic pollution in the past 1000 years based on Lake Huguangyan sediments, Southern China | |
| Chien et al. | Evaluation of atmospheric dry deposition as a source of nutrients and trace metals to Lake Tahoe | |
| Erdman et al. | Variation in the element content of Parmelia chlorochroa from the Powder River Basin of Wyoming and Montana | |
| CN104568675A (en) | DGT testing analysis method for phosphorus absorption characteristics of lake aquatic plant root zone | |
| Zhang et al. | Seasonal variations of cave dripwater hydrogeochemical parameters and δ13CDIC in the subtropical monsoon region and links to regional hydroclimate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |