CN104561159A - Fermentation method for efficient coproduction of poly-gamma-glutamic acid and 2,3-butanediol by virtue of bacillus - Google Patents
Fermentation method for efficient coproduction of poly-gamma-glutamic acid and 2,3-butanediol by virtue of bacillus Download PDFInfo
- Publication number
- CN104561159A CN104561159A CN201310514337.XA CN201310514337A CN104561159A CN 104561159 A CN104561159 A CN 104561159A CN 201310514337 A CN201310514337 A CN 201310514337A CN 104561159 A CN104561159 A CN 104561159A
- Authority
- CN
- China
- Prior art keywords
- glutamic acid
- bacillus
- gamma
- poly
- butanediol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of microbial fermentation and particularly relates to a batch fermentation method for efficient coproduction of poly-gamma-glutamic acid and 2,3-butanediol by virtue of bacillus. According to the method, by liquid-submerged fermentation, two products, namely, poly-gamma-glutamic acid and 2,3-butanediol are simultaneously produced. The process can be used for safely producing the strain, the production cost is effectively reduced, the highest concentrations of simultaneously produced poly-gamma-glutamic acid and 2,3-butanediol can respectively reach 52.2g/L and 73.68g/L, the apparent conversion rate of glucose reaches 83.9%, the production strength reaches 2.10g.L<-1>.h<-1>, and the method is suitable for industrial production.
Description
Technical field
The invention belongs to technical field of microbial fermentation, be specifically related to a kind of method utilizing bacillus batch fermentation to come efficient coproduction poly-gamma-glutamic acid and 2,3-butanediol.
Background technology
Poly-gamma-glutamic acid (poly γ-glutamic acid, γ-PGA) be the same polymeric amide be connected to form by L-and D-Glu, be a kind of can through the polymer extracellular polymeric of Microbe synthesis, average relative molecular mass is generally between 10-10000 kD.γ-PGA has water-soluble, high viscosity, film-forming properties and moisture retention etc., and to human body and environment toxicological harmless.Therefore, be with a wide range of applications in fields such as medicine manufacture, environmental improvement, light industry and agriculturals.Because the microorganism screened at present exists the various problems such as γ-PGA productive rate is low, raw materials cost is high, industrial applications is subject to serious restriction.How to reduce fermentation costs, or improve the added value of γ-PGA fermenting process, will the competitive power improving γ-PGA related products be contributed to.
2,3-butyleneglycol, as a kind of important industrial chemicals and liquid fuel, is widely used in the fields such as the chemical industry energy and food, has wide prospects for commercial application, seek economical and efficient and oligosaprobic 2,3-butanediol preparation method and its industrialization is the focus of various countries' scientific research always the most at last.But 2, the theoretical yield of 3-butyleneglycol to glucose is only about 0.5 g/g, and current high yield 2, the bacterial strain of 3-butyleneglycol is mostly pathogenic bacteria, as Serratia marcesens, enteroaerogen, Klebsiella etc., and the principle of sound accounting of large-scale industrial production limits the application that these produce bacterial strain.
Through retrieval, existing report technology is only produced separately poly-gamma-glutamic acid or is produced separately a kind of product of 2,3-butanediol, has that raw material availability is low, high in cost of production problem.Late Cambrian of the present invention one utilizes bacillus high yield poly-gamma-glutamic acid and 2 simultaneously, the novel method of 3-butyleneglycol, the added value of γ-PGA fermenting process can be improved, wherein 2,3-butyleneglycol production peak reaches 73.68 g/L, the yield of fermentation gross product to sugar reaches 0.84 g/g, and the bacillus licheniformis used and Bacillus subtillis are grade-safe microorganism, have industrial applications potentiality.
Summary of the invention
The present invention is for the purpose of the added value improving γ-PGA fermenting process, by bacillus fermentation coproduction poly-gamma-glutamic acid and 2,3-butanediol, produces poly-gamma-glutamic acid and 2,3-butanediol simultaneously, has wide industrial applications prospect.
The object of the invention is to be achieved through the following technical solutions:
A, production bacterial classification of the present invention adopt bacillus licheniformis (the bacillus licheniformis WX-02 of freezing, Hubei China is delivered on April 28th, 2008, China typical culture collection center preservation in Wuhan University, deposit number is CCTCCNO:M 208065) and Bacillus subtillis (Bacillus subtillis X-003, Hubei China is delivered on December 25th, 2002, China typical culture collection center preservation in Wuhan University, deposit number is CCTCC NO:M 202048).
B, preparation seed liquor:
After the bacterial classification of above-mentioned freezing is activated on solid LB media flat board, be transferred to LB liquid medium and spread cultivation, obtain seed liquor.Described activation culture temperature is 37 DEG C, incubation time 12-24h; The described culture temperature that spreads cultivation is 37 DEG C, shaking speed 200r/min, cultivates 8-12h; The formula of described solid LB media is (g/L): peptone 10, yeast extract 5, NaCl10, agar 15, pH7.0; The formula of described LB liquid medium is (g/L): peptone 10, yeast extract 5, NaCl10, pH7.0.
C, batch fermentation are cultivated:
(1) prepare coproduction fermention medium, it consists of (g/L): glucose 70-170, Sodium Glutamate 10-80, SODIUMNITRATE 0-15, K
2hPO
43H
2o0.5-2, MgSO
47H
2o0.5-2, CaCl
22H
2o0.05-1.5, ZnSO
40.05-1.5, MnSO
40.05-0.15, initial pH value is 7.0-7.5, and initial pH is 7.0-7.5.
(2) triangular flask adds substratum by the liquid amount of 100mL/500mL, 115 DEG C of sterilizing 30min, and inoculum size is 3%, 37 DEG C and cultivates 30-60h; Fermentor tank adds fermention medium by the liquid amount of 50-80%, 115 DEG C of sterilizing 30min, cultivates 30-60h, ventilation ratio 1:0.5-2(V/V for 37 DEG C).
Wherein the preferred concentration of glucose is 70-170g/L, and the preferred concentration of Sodium Glutamate is 10-80g/L, and the preferred concentration of SODIUMNITRATE is 5-15g/L, and preferred fermented incubation time is 30-60h.
The present invention has following outstanding feature:
(1) the present invention proposes the efficient co-production of poly-gamma-glutamic acid and 2,3-butanediol first, and angle is novel, economical and efficient.
(2) the present invention produces 2,3-butanediol by aliment security level bacterial strain, for the further industrial application of 2,3-butanediol is laid a good foundation.
(3) fermention medium raw materials cost is low, poly-gamma-glutamic acid and 2,3-butanediol coproduction gain in yield remarkable.
Embodiment
The present invention is described in detail by the following examples, but all embodiments do not form any restriction to the present invention.
Embodiment 1 uses bacillus licheniformis with fermention medium 1 shake flask fermentation coproduction poly-gamma-glutamic acid and 2,3-butanediol
(1) bacterial strain is produced with the bacterial classification bacillus licheniformis WX-02 (CCTCC NO:M208065) of freezing.
(2) slant strains activation:
By the bacillus licheniformis WX-02 of freezing, be inoculated into LB solid slope (containing peptone 10g/L, yeast extract 5g/L, NaCl10g/L, agar 15g/L, pH7.0), cultivate 24h, obtain activated spawn for 37 DEG C.
(3) seed liquor is cultivated:
Be transferred to containing peptone 10g/L by the kind daughter bacteria that LB solid slope activates, yeast extract 5g/L, NaCl10g/L, pH are in the LB triangular flask liquid seed culture medium of 7.0, in 37 DEG C, cultivate 10h to mid log phase under 200r/min.(4) Shake flask batch fermentation culture:
Above-mentioned fermention medium 1 compound method is as follows: glucose 90g/L, Sodium Glutamate 30g/L, Trisodium Citrate 10g/L, ammonium chloride 8g/L, MnSO
4h
2o0.1g/L, MgSO
47H
2o1.5g/L, K
2hPO
41.5g/L, ZnSO
47H
2o1.5g/L, CaCl
22H
2o1.5g/L, pH7.0, supplement distilled water to 1L, by the triangular flask of described fermention medium packing 500mL, every bottled liquid measure 100mL, 115 DEG C of sterilizing 30min, be cooled to 37 DEG C, according to the inoculum size access seed liquor of 1.5ml, shaking speed 200r/min, leavening temperature 37 DEG C, ferments to 36h and terminates to ferment.
(5) mensuration of poly-gamma-glutamic acid content:
By centrifugal for above-mentioned fermented liquid removing thalline, collect supernatant liquor, get 5mL centrifuged supernatant, add 3 times of volume alcohol precipitation poly-gamma-glutamic acids, lysate, adds equal-volume concentrated hydrochloric acid, be sealed in boiling water bath and be hydrolyzed 24h, added by hydrolyzed solution in the NaOH of 10mol/L after hydrolysis and constant volume, through the filtering with microporous membrane of 0.22u m, the content measuring poly-gamma-glutamic acid is completed by high pressure liquid chromatograph (HPLC), chromatographic column Agilent1100, moving phase is 100mmol/L KH
2pO
4add 5.0% methyl alcohol (with phosphoric acid adjust pH to 2.5) to filter, ultrasonic degassing, ultraviolet detection wavelength is 210nm, and flow velocity is 1mL/min, and L-glutamic acid is as quantitative standard substance.Calculate according to following publicity: aminoglutaric acid concentration × 129/147 after poly-gamma-glutamic acid concentration=hydrolysis.
(6) detection of 2,3-butanediol content:
Get above-mentioned fermented liquid 0.6mL and add 3mL ethyl acetate (propyl carbinol containing 1g/L is interior mark), abundant concussion, add 2.17g anhydrous magnesium sulfate drying again, abundant mixing, static extracting 2h, centrifuging and taking supernatant, through 0.22 μm of organic phase filtering with microporous membrane, measures 2, the content of 3-butyleneglycol is completed by gas chromatograph, gas chromatograph Trace GC Ultra(Thermo company) detect.Gas chromatograph parameters is as follows: hydrogen flame detector, TR-WAX chromatographic column (30m × 0.32mm ID, 0.25um Film), nitrogen does carrier gas, flow is 1.0mL/min, sample size 1 μ l, Splitless injecting samples, sampler temperature 215 DEG C, detector temperature 245 DEG C, column oven heating schedule: 50 DEG C keep 2min, is warming up to 110 DEG C with 10 DEG C/min and keeps 0.5min, be warming up to 150 DEG C with 5 DEG C/min and keep 0.5min, be warming up to 220 DEG C with 20 DEG C/min and keep 1min.
The content that measurement result obtains poly-gamma-glutamic acid in fermented liquid is 12.5g/L, and the content of 2,3-butanediol is 46.21g/L.
Embodiment 2 uses bacillus licheniformis with fermention medium 2 shake flask fermentation coproduction poly-gamma-glutamic acid and 2,3-butanediol
(1) the production bacterial classification of the present embodiment employing is with embodiment 1 (1).
(2) slant strains activation is with embodiment 1 (2).
(3) seed liquor is cultivated with embodiment 1 (3).
(4) Shake flask batch fermentation culture:
Above-mentioned fermention medium 2 compound method is as follows: glucose 90g/L, Sodium Glutamate 30g/L, SODIUMNITRATE 10g/L, Trisodium Citrate 10g/L, ammonium chloride 8g/L, MnSO
4h
2o0.15g/L, MgSO
41g/L, K
2hPO
43H
2o1g/L, ZnSO
47H
2o1g/L, CaCl
21g/L, pH7.0, supplement distilled water to 1L, by the triangular flask of described fermention medium packing 500mL, every bottled liquid measure 100mL, 115 DEG C of sterilizing 30min, be cooled to 37 DEG C, according to the inoculum size access seed liquor of 1.5ml, shaking speed 200r/min, leavening temperature 37 DEG C, ferments to 48h and terminates to ferment.
(5) mensuration of poly-gamma-glutamic acid content is with embodiment 1 (5).
(6) detection of 2,3-butanediol content is with embodiment 1 (6).
Finally obtaining poly-gamma-glutamic acid content in fermented liquid is 40.6g/L, and the content of 2,3-butanediol is 34.68g/L.Comparative example 1 is visible, adds the SODIUMNITRATE of 10g/L in fermention medium, and poly-gamma-glutamic acid output increases by 225%, poly-gamma-glutamic acid and 2,3-butanediol output and increase by 28%.
Embodiment 3 uses bacillus licheniformis with fermention medium 3 shake flask fermentation coproduction poly-gamma-glutamic acid and 2,3-butanediol
(1) the production bacterial classification of the present embodiment employing is with embodiment 1 (1).
(2) slant strains activation is with embodiment 1 (2).
(3) seed liquor is cultivated with embodiment 1 (3).
(4) Shake flask batch fermentation culture:
Above-mentioned fermention medium 3 compound method is as follows: glucose 90g/L, Sodium Glutamate 80g/L, SODIUMNITRATE 10g/L, Trisodium Citrate 10g/L, ammonium chloride 8g/L, MnSO
4h
2o0.15g/L, MgSO
40.5g/L, K
2hPO
43H
2o0.5g/L, ZnSO
47H
2o0.5g/L, CaCl
20.5g/L, pH7.0, supplement distilled water to 1L, by the triangular flask of described fermention medium packing 500mL, every bottled liquid measure 100mL, 115 DEG C of sterilizing 30min, be cooled to 37 DEG C, according to the inoculum size access seed liquor of 1.5ml, shaking speed 200r/min, leavening temperature 37 DEG C, ferments to 36h and terminates to ferment.
(5) mensuration of poly-gamma-glutamic acid content is with embodiment 1 (5).
(6) detection of 2,3-butanediol content is with embodiment 1 (6).
Poly-gamma-glutamic acid output 35.8g/L after fermentation ends, 2,3-butanediol output 28.91g/L.
Embodiment 4 uses bacillus licheniformis with fermention medium 4 shake flask fermentation coproduction poly-gamma-glutamic acid and 2,3-butanediol
(1) the production bacterial classification of the present embodiment employing is with embodiment 1 (1).
(2) slant strains activation is with embodiment 1 (2).
(3) seed liquor is cultivated with embodiment 1 (3).
(4) Shake flask batch fermentation culture:
Above-mentioned fermention medium 4 compound method is as follows: Sodium Glutamate 40g/L, glucose 150g/L, SODIUMNITRATE 10g/L, Trisodium Citrate 10g/L, ammonium chloride 8g/L, MnSO
4h
2o0.15g/L, MgSO
47H
2o1g/L, K
2hPO
43H
2o1g/L, ZnSO
47H
2o1g/L, CaCl
21g/L, pH7.0, supplement distilled water to 1L, by the triangular flask of described fermention medium packing 500mL, every bottled liquid measure 100mL, 115 DEG C of sterilizing 30min, be cooled to 37 DEG C, according to the inoculum size access seed liquor of 1.5ml, shaking speed 200r/min, leavening temperature 37 DEG C, ferments to 60h and terminates to ferment.
(5) mensuration of poly-gamma-glutamic acid content is with embodiment 1 (5).
(6) detection of 2,3-butanediol content is with embodiment 1 (6).
Result poly-gamma-glutamic acid and 2,3-butanediol output are respectively 52.2g/L and 73.68g/L.Comparative example 2 finds out, glucose concn increases to 150g/L, and make poly-gamma-glutamic acid and 2,3-butanediol ultimate production increase by 75%, glucose apparent conversion reaches 83.9%, and wherein, sugar rises to 53% to the apparent conversion of 2,3-butanediol.
Embodiment 5 uses bacillus licheniformis with fermention medium 2 in 50L tank batch fermentation coproduction poly-gamma-glutamic acid and 2,3-butanediol
(1) the production bacterial classification of the present embodiment employing is with embodiment 1 (1).
(2) slant strains activation is with embodiment 1 (2).
(3) seed liquor is cultivated with embodiment 1 (3).
(4) 50L fermentor tank batch culture:
Above-mentioned fermention medium 2 compound method is as follows: Sodium Glutamate 30g/L, glucose 110g/L, SODIUMNITRATE 10g/L, Trisodium Citrate 10g/L, ammonium chloride 8g/L, MnSO
4h
2o0.15g/L, MgSO
47H
2o1g/L, K
2hPO
41g/L, ZnSO
47H
2o1g/L, CaCl
22H
2o1g/L, pH7.0, the 30L of this substratum preparation in the present embodiment, drops into the fermentor tank of 50L, 115 DEG C of sterilizing 30min by described fermention medium, be cooled to 37 DEG C, according to the inoculum size access seed liquor of 900ml, mixing speed 200-800r/min progressively improves, air flow 1:1(V/V), leavening temperature 37 DEG C, ferments to 36h and terminates to ferment.
(5) mensuration of poly-gamma-glutamic acid content is with embodiment 1 (5).
(6) detection of 2,3-butanediol content is with embodiment 1 (6).
The content finally obtaining poly-gamma-glutamic acid and 2,3-butanediol in fermented liquid is respectively 38.3g/L and 57.50g/L, and wherein sugar rises to 52% to the apparent conversion of 2,3-butanediol.
Embodiment 6 uses Bacillus subtillis with fermention medium 3 shake flask fermentation coproduction poly-gamma-glutamic acid and 2,3-butanediol
(1) bacterial strain is produced with the species Bacillus bacillus X-003 (CCTCC NO:M202048) of freezing.
(2) slant strains activation is with embodiment 1 (2).
(3) seed liquor is cultivated with embodiment 1 (3).
(4) shake flask fermentation is cultivated:
According to following component preparation fermention medium: glucose 90g/L, Sodium Glutamate 80g/L, SODIUMNITRATE 10g/L, Trisodium Citrate 10g/L, ammonium chloride 8g/L, MnSO
4h
2o0.15g/L, MgSO
41g/L, K
2hPO
43H
2o1g/L, ZnSO
47H
2o1g/L, CaCl
21g/L, pH7.0, supplement distilled water to 1L, by the triangular flask of described fermention medium packing 500mL, every bottled liquid measure 100mL, 115 DEG C of sterilizing 30min, be cooled to 37 DEG C, according to the inoculum size access seed liquor of 1.5ml, shaking speed 200r/min, leavening temperature 37 DEG C, ferments to 36h and terminates to ferment.
(5) mensuration of poly-gamma-glutamic acid content is with embodiment 1 (5).
(6) detection of 2,3-butanediol content is with embodiment 1 (6).
The content finally obtaining poly-gamma-glutamic acid and 2,3-butanediol in fermented liquid is respectively 29.6g/L and 28.18g/L.
Claims (5)
1. one kind utilizes bacillus fermentation coproduction poly-gamma-glutamic acid and 2, the method of 3-butyleneglycol, it is characterized in that described bacillus comprises bacillus licheniformis WX-02(deposit number is CCTCC NO:M208065) or Bacillus subtillis X-003(deposit number be CCTCC NO:M202048).
2. the method utilizing bacillus batch fermentation coproduction poly-gamma-glutamic acid and 2,3-butanediol according to claim 1, is characterized in that it comprises the following steps:
Bacillus licheniformis (the bacillus licheniformis WX-02 of a, employing freezing, be preserved in China typical culture collection center, be numbered CCTCC NO:M208065) or Bacillus subtillis (Bacillus subtillis X-003, be preserved in China typical culture collection center, be numbered CCTCC NO:M202048) as producing bacterial classification;
B, preparation seed liquor:
By the strain inoculation of above-mentioned freezing on LB solid medium (g/L: peptone 10, yeast extract 5, NaCl10, agar 15, pH7.0) flat board, 37 DEG C of constant temperature culture 12-24h, make it activate; Be transferred to by the seed of above-mentioned activation in LB liquid seed culture medium (g/L: peptone 10, yeast extract 5, NaCl10, pH7.0), 37 DEG C, 200r/min cultivates 8-12h again;
C, batch fermentation are cultivated:
(1) prepare coproduction fermention medium, it consists of (g/L): glucose 70-170, Sodium Glutamate 10-80, SODIUMNITRATE 0-15, K
2hPO
43H
2o0.5-2, MgSO
47H
2o0.5-2, CaCl
22H
2o0.05-1.5, ZnSO
40.05-1.5, MnSO
40.05-0.15, initial pH value is 7.0-7.5;
(2) liquid amount of 50-100mL/500mL is pressed in triangular flask fermentation, and load above-mentioned substratum, 115 DEG C of sterilizing 30min, inoculum size is 1-3%, cultivates 30-60h for 37 DEG C; Fermentor tank drops into above-mentioned fermention medium by the liquid amount of the 50-80% of volume, 115 DEG C of sterilizing 30min, access seed liquor; Cultivate 30-60h, ventilation ratio 1: 0.5-2(V/V for 37 DEG C).
3. method according to claim 1, is characterized in that in described fermention medium, sodium nitrate concentration is 5-15g/L.
4. method according to claim 1, is characterized in that described fermention medium Glutamic Acid na concn is 10-80g/L.
5. method according to claim 1, is characterized in that in described fermention medium, glucose concn is 70-170g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310514337.XA CN104561159A (en) | 2013-10-25 | 2013-10-25 | Fermentation method for efficient coproduction of poly-gamma-glutamic acid and 2,3-butanediol by virtue of bacillus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310514337.XA CN104561159A (en) | 2013-10-25 | 2013-10-25 | Fermentation method for efficient coproduction of poly-gamma-glutamic acid and 2,3-butanediol by virtue of bacillus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104561159A true CN104561159A (en) | 2015-04-29 |
Family
ID=53078236
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310514337.XA Pending CN104561159A (en) | 2013-10-25 | 2013-10-25 | Fermentation method for efficient coproduction of poly-gamma-glutamic acid and 2,3-butanediol by virtue of bacillus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104561159A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107502585A (en) * | 2017-09-06 | 2017-12-22 | 武汉骏安生物科技有限公司 | One plant of bacillus licheniformis engineering bacteria for efficiently synthesizing poly- γ glutamic acid |
| CN109988788A (en) * | 2019-04-24 | 2019-07-09 | 武汉骏安生物科技有限公司 | A method of promoting bacillus licheniformis high yield polyglutamic acid sodium |
| CN112870151A (en) * | 2021-02-08 | 2021-06-01 | 武汉骏安生物科技有限公司 | Skin barrier repair composition containing sodium polyglutamate and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1536071A (en) * | 2003-04-10 | 2004-10-13 | 华中农业大学 | Poly-gamma-glutamic acid generation bacteria and method for producing poly-gamma-glutamic acid |
| CN101603015A (en) * | 2008-06-13 | 2009-12-16 | 河北维尔康制药有限公司 | A kind of lichem bacillus strain and purposes and the method for gathering gamma-glutamic acid with its production |
| CN101851598A (en) * | 2010-05-10 | 2010-10-06 | 江南大学 | Environmentally-friendly breeding of bacillus subtilis for producing 2,3-butanediol by fermentation with glucose substrate |
| CN102391970A (en) * | 2011-11-18 | 2012-03-28 | 山东大学 | Method for producing 2, 3-butanediol and special bacillus licheniformis thereof |
-
2013
- 2013-10-25 CN CN201310514337.XA patent/CN104561159A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1536071A (en) * | 2003-04-10 | 2004-10-13 | 华中农业大学 | Poly-gamma-glutamic acid generation bacteria and method for producing poly-gamma-glutamic acid |
| CN101603015A (en) * | 2008-06-13 | 2009-12-16 | 河北维尔康制药有限公司 | A kind of lichem bacillus strain and purposes and the method for gathering gamma-glutamic acid with its production |
| CN101851598A (en) * | 2010-05-10 | 2010-10-06 | 江南大学 | Environmentally-friendly breeding of bacillus subtilis for producing 2,3-butanediol by fermentation with glucose substrate |
| CN102391970A (en) * | 2011-11-18 | 2012-03-28 | 山东大学 | Method for producing 2, 3-butanediol and special bacillus licheniformis thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107502585A (en) * | 2017-09-06 | 2017-12-22 | 武汉骏安生物科技有限公司 | One plant of bacillus licheniformis engineering bacteria for efficiently synthesizing poly- γ glutamic acid |
| CN109988788A (en) * | 2019-04-24 | 2019-07-09 | 武汉骏安生物科技有限公司 | A method of promoting bacillus licheniformis high yield polyglutamic acid sodium |
| CN109988788B (en) * | 2019-04-24 | 2022-11-15 | 武汉骏安生物科技有限公司 | Method for promoting high yield of sodium polyglutamate by bacillus licheniformis |
| CN112870151A (en) * | 2021-02-08 | 2021-06-01 | 武汉骏安生物科技有限公司 | Skin barrier repair composition containing sodium polyglutamate and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102220248B (en) | Bacterial strain for producing PMLA [Poly (Beta-L-malic acid)] and method for producing PMLA by fermentation of bacterial strain | |
| CN102911974B (en) | Method for producing gamma-polyglutamic acid though hot fermentation of bacillus subtilis | |
| CN103865855A (en) | Bacillus subtilis strain and applications thereof | |
| CN103789363B (en) | Improve the method for polymalic acid output | |
| CN104232504A (en) | Strain for synthesizing gamma-polyglutamic acid and method for efficiently preparing gamma-polyglutamic acid by using strain | |
| CN102533885B (en) | Method for producing gamma-polyglutamic acid through adding sodium chloride (NaCl) in fermentation process | |
| CN102994467A (en) | L-d-glutamic oxidase with substrate specificity and alpha-oxoglutarate produced by catalysis of same | |
| CN102586141B (en) | Method for preparing nattokinase ferment bacteria | |
| CN104561159A (en) | Fermentation method for efficient coproduction of poly-gamma-glutamic acid and 2,3-butanediol by virtue of bacillus | |
| CN105017086A (en) | Separation and purification method for L-citrulline | |
| CN101550431B (en) | Method for preparing 2, 3-butanediol using xylose mother liquor | |
| CN103805651B (en) | A kind of pulullan polysaccharide production method based on cellular metabolism regulating strategy | |
| CN103627698A (en) | Breeding of acetoin high-tolerance bacterial strain and acetoin fermentation production with bacterial strain | |
| CN108220352A (en) | A kind of method of raw material fermentation production gamma-polyglutamic acid | |
| CN108949851B (en) | Method for producing glucuronic acid through fermentation of zygosaccharomyces rouxii | |
| CN104651284A (en) | Sphingomonas sp. T-3 and method for producing biological polysaccharide and poly-beta-hydroxy-butanoic acid by co-fermentation | |
| CN105176859A (en) | Strain MQO-153 for production of arginine deiminase | |
| CN107760732A (en) | A kind of production method of agriculture level γ polyglutamic acids | |
| CN104560831B (en) | A kind of efficiently Antarctic Sea Ice bacterium of the production with immunocompetent exocellular polysaccharide | |
| CN104357365A (en) | Gluconacetobacter saccharivorans strain capable of producing free glucuronic acid | |
| CN103667153B (en) | Poly(3-hydroxybutyrate) generating thermophiles | |
| CN110042129A (en) | A kind of preparation method of agriculture gamma-polyglutamic acid | |
| CN109207530A (en) | A method of dihydroxyacetone (DHA) is produced with Japanese gluconobacter suboxydans strain HD 1025 | |
| CN101701243A (en) | Method for producing R-mandelic acid and derivates thereof by biocatalysis | |
| CN104611387A (en) | Method for production of L-dopa melanin by fermentation of Streptomyces sp. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150429 |
|
| RJ01 | Rejection of invention patent application after publication |