CN104560792A - Amylase-producing strain and application thereof - Google Patents
Amylase-producing strain and application thereof Download PDFInfo
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- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
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Abstract
The invention discloses an amylase-producing strain Phaeocystidibacter merougensis G18 with a collection number of CGMCC No.9861. The strain is separated from red sea extracts, the optimal reaction conditions of the produced amylase refer to the temperature of 70 to 75 DEG C and the pH value of 8.0-8.5, and the strain has a certain thermal stability and acid-alkali stability. The invention also discloses a culture method and application of the strain Phaeocystidibacter merougensis G18, a method for producing the amylase by using the strain, and the amylase. The strain and the produced amylase disclosed by the invention have wide application prospects in the industries such as medicines, chemical industry and foods.
Description
Technical field
The present invention generally relates to a kind of new bacterial strain, more specifically, relates to one and derives from the diastatic bacterial strain of the sedimental product in Red sea and its application.
Background technology
Amylase (amylase) refers to can hydrolyzed starch, and the enzyme general name of the related polysaccharides such as glycogen, is applied to the industries such as starch syrup, traditional fermentation, food-processing, weaving destarch, is one of maximum zymin of the widest output of purposes.Microorganism is one of main source of industrial starch enzyme, and known numerous bacterium and fungi can produce amylase, and it is in the majority to produce diastatic research report with bacterial metabolism in bacillus (Bacillus).Wherein, Bacillus coagulans (Bacillus coagulans), subtilis (B.subtilis), bacillus acidocldarius (B.stearothermophilus), Bacillus licheniformis (B.licheninformis) etc. all can produce alpha-amylase.
Amylase has a wide range of applications field, as: be applied to starch processing industry and manufacture glucose, maltose etc.; Be applied to foodstuffs industry and make fruit juice, spices, monosodium glutamate, bread etc.; Be applied to and brewage fermentation industry making white wine, beer, soy sauce, vinegar etc.; Be applied to field of medicaments and prepare diagnostic enzyme, digestant etc.But application requiring amylase not only wants vigor high so widely, different ph stability and thermostability also to be possessed to adapt to the demand of different industries.Therefore, the diastatic strain excellent of screening product different qualities is still very necessary.
Summary of the invention
The object of the present invention is to provide a kind of newly can produce diastatic bacterial strain, and provide purposes and the cultural method of this bacterial strain, and the amylase utilizing this bacterial strain to produce diastatic method and produce.
According to a first aspect of the invention, a kind of new bacterial strain Phaeocystidibacter merougensis G18 is provided.This strains separation is from Red sea settling (erect-position: 38 ° 53.8701 ', N22 ° 16.9540 '), and be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (China General Microbiological Culture Collection Center on October 27th, 2014, CGMCC), address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and deposit number is: CGMCC No.9861.Its 16S rDNA sequence as shown in the SEQ ID NO:1 in sequence table, with the phylogenetic tree of its 16S rDNA sequence construct as shown in Figure of description 1.This bacterial strain is Gram-negative bacteria, aerobic growth, and thalline is bar-shaped (wide about 0.3 ~ 0.5 μm, be about 0.5 ~ 5.5 μm).Bacterium colony is in yellow, and smooth surface is moistening, and central authorities are slightly convex.The most suitable growth pH and temperature are respectively 6.0 ~ 8.0,28 ~ 37 DEG C.The most suitable growth NaCl concentration is 2 ~ 5% (mass volume ratios).The most suitable growth Mg
2+concentration is: 0.005 ~ 0.05mol/L, the most suitable growth Ca
2+concentration is 0.005 ~ 0.02mol/L.Unfermentable glucose produces acid, can not caseinhydrolysate, chitin, tween 80, hydrolyzable gelatin and starch.Alkaline phosphatase, acid phosphatase, esterase, leucine aminopeptidase, α-amino-isovaleric acid aminopeptidase, CAP, trypsinase, naphthol phosphoric acid lytic enzyme, beta-galactosidase enzymes are positive, and lipase, alpha-chymotrypsin, alpha-galactosidase, beta-glucuronidase, alpha-glucosidase, beta-glucosidase, N-acet-beta-amino glucosidase, alpha-Mannosidase, Alpha-Fucosidase are negative.
The present inventor finds that this bacterial strain Phaeocystidibacter merougensis G18 can produce amylase under containing the culture environment of starch.This diastatic reaction conditions is pH 8.0 ~ 9.5,70 ~ 75 DEG C, and optimum condition is pH 8.0, and temperature is 70 DEG C, belongs to a kind of alpha-amylase.Further research finds, the amylase that this bacterium produces stable in properties under (4.5 ~ 10.5) and comparatively high temps (as thermal treatment at 65 DEG C 45 minutes) within the scope of comparatively wide in range pH, show gratifying activity, be with a wide range of applications.
Therefore, according to a second aspect of the invention, provide described bacterial strain Phaeocystidibacter merougensis G18 for the production of diastatic purposes.
According to a third aspect of the invention we, provide a kind of and produce diastatic method.Described method comprises the step utilizing bacterial strain Phaeocystidibacter merougensis G18 to carry out fermenting and the diastatic step extracted in fermented liquid.Constant temperature culture under the condition of fermentation pH 6.0 ~ 8.0,28 ~ 37 DEG C in fermentation culture.Described fermentation culture comprises in often liter of seawater, 2 ~ 10g starch, 5 ~ 15g Tryptones and 2 ~ 5g yeast powder.
Supernatant liquor is obtained by centrifugal method when the bacteria concentration in nutrient solution reaches certain value, and by the amylase in ordinary method Isolation and purification supernatant liquor.These methods are all [1-3] well known to those skilled in the art, do not repeat them here.
According to last aspect of the present invention, provide the amylase obtained by aforesaid method.
Bacterial strain Phaeocystidibacter merougensis G18 medium component of the present invention is simple, and culture condition is loose, and increment is fast.Add starch in the medium and can induce generation amylase.The amylase produced can in pH8.0 ~ 9.5,70 ~ 75 DEG C, preferred pH8.0, temperature are react under the condition of 70 DEG C.There is wider tolerance range to temperature and pH value, greater activity can be kept in the scope of pH 4.5 ~ 10.5 and under comparatively high temps (as thermal treatment at 65 DEG C 45 minutes).Therefore, bacterial strain of the present invention and the amylase produced by this bacterial strain, in numerous fields and industry such as medicine, chemical industry and food, particularly experience pH change and/or compared with in those techniques of high processing temperature, have broad application prospects.
Accompanying drawing explanation
Fig. 1 is the phylogenetic tree of Phaeocystidibacter merougensis G18.
Fig. 2 produces by bacterial strain Phaeocystidibacter merougensis G18 amylase relative reactivity change curve at different temperatures.
Fig. 3 for treatment of different temperature bacterial strain Phaeocystidibacter merougensis G18 produce the influence curve figure of amylase to its activity.
Fig. 4 is produced amylase relative reactivity change curve under condition of different pH by bacterial strain Phaeocystidibacter merougensis G18.
Fig. 5 at condition of different pH process bacterial strain Phaeocystidibacter merougensis G18 produce the influence curve figure of amylase to its activity.
Embodiment
Following examples further illustrate of the present invention, but the invention is not restricted to following embodiment.
In following examples amylase activity measure and characterizing method as follows:
DNS (3,5-Dinitrosalicylic acid, 3,5-dinitrosalicylic acid) method is utilized to measure amylase activity.In the basic conditions, dinitrosalicylic acid (DNS) and reducing sugar generation redox reaction, 3-amino-the 5-NITROSALICYLIC ACID generated is aobvious red-brown under boiling conditions, and within the scope of finite concentration shade and the proportional relation of reducing sugar content, available colorimetric method for determining reducing sugar content.
1 Ge Meihuo unit (U) is defined as: 70 DEG C, under pH8.0 condition, in 1min, enzymatic starch generates the enzyme amount needed for 1 μ g reducing sugar (with glucose as a standard).
The preparation of DNS reagent: get after appropriate amount of deionized water boils 10min and be cooled to room temperature, for DNS preparation of reagents.Take 6.3g 3,5-dinitrosalicylic acid to be dissolved in a small amount of water, add 21.0g sodium hydroxide and be fully dissolved in about 500mL water, dissolve 182.0g sodium tartrate successively again, 5.0g phenol, 5.0g S-WAT, stores after constant volume 1L in brown bottle and is positioned over 4 DEG C of preservations, for subsequent use.
The making of glucose standard curve: aseptic deionized water prepares the glucose solution of 0.1,0.25,0.5,0.75,1 and 1.25 μ g/ μ L respectively.Take aseptic deionized water as blank, respectively establish 3 parallel, get the above-mentioned solution of 250 μ L respectively and mix 5min in rearmounted boiling water bath with 450 μ L DNS solution, be cooled to room temperature, get 50 μ L reaction solutions in 384 orifice plates, measure 540nm place absorbancy (Eon microplate spectrophotometer, Bio Tek company of the U.S.).Being ordinate zou with glucose quality, take absorbancy as X-coordinate, drawing standard curve.
The isolation identification of embodiment 1:Phaeocystidibacter merougensis G18
Get 5g Red sea sediment sample (erect-position: 38 ° 53.8701 ', N 22 ° 16.9540 '), put into the 250mL triangular flask that 100mL 3% (quality volume fraction) aseptic sodium chloride solution and granulated glass sphere are housed, be placed in 200rpm shaking table concussion 30min, make the dispersed rear standing 10min of soil sample.10 times of dilution methods, the supernatant after coating leaves standstill, in substratum MA (5g Tryptones, 2g yeast powder, 10g agar, 1L seawater) solid plate, is placed in 37 DEG C and cultivates 48h.The dissimilar single bacterium colony of picking lines MA solid plate repeatedly, is placed in 37 DEG C and cultivates 48h.Obtain the bacterial strain of pure culture in triplicate and 16S rDNA order-checking qualification is carried out to it.Concrete grammar can reference [4].
The described bacterial strain G18 that purifying obtains, through 16S rDNA sequence alignment, has the highest similarity (being 94.8%) with Phaeocystidibacter luteus PG2S01, called after Phaeocystidibacter merougensis G18.The 16S rDNA of this bacterial strain asks for an interview sequence table [sequence numbering No.1].
The utilization of carbon source type of bacterial strain G18 is measured by U.S. Biolog GEN III carbon source automatic analysis identification systems, physiological and biochemical property utilizes French Mei Liai company (bioMe ' rieux) API test kit (to comprise API ZYM, API 20NE, API 20E and API 50CH) measure, above test kit all has detailed operation instructions and its using method is well known to those skilled in the art, and repeats no more.Optimum growth temperature, pH, NaCl concentration, Mg
2+concentration, Ca
2+concentration, and casein, chitin, tween 80 and Starch Hydrolysis characteristic detecting method, can reference [5].After testing, this bacterial strain is Gram-negative bacteria, aerobic growth, and thalline is bar-shaped (wide about 0.3 ~ 0.5 μm, be about 0.5 ~ 5.5 μm).Bacterium colony is in yellow, and smooth surface is moistening, and central authorities are slightly convex.The most suitable growth pH and temperature are respectively 6.0 ~ 8.0,28 ~ 37 DEG C.The most suitable growth NaCl concentration is 2 ~ 5% (mass volume ratios).The most suitable growth Mg
2+concentration is: 0.005 ~ 0.05mol/L, the most suitable growth Ca
2+concentration is 0.005 ~ 0.02mol/L.Unfermentable glucose produces acid, can not caseinhydrolysate, chitin, tween 80, hydrolyzable gelatin and starch.Alkaline phosphatase, acid phosphatase, esterase, leucine aminopeptidase, α-amino-isovaleric acid aminopeptidase, CAP, trypsinase, naphthol phosphoric acid lytic enzyme, beta-galactosidase enzymes are positive, and lipase, alpha-chymotrypsin, alpha-galactosidase, beta-glucuronidase, alpha-glucosidase, beta-glucosidase, N-acet-beta-amino glucosidase, alpha-Mannosidase, Alpha-Fucosidase are negative.
4mL MA (5g Tryptones is equipped with in the mono-bacterium colony access of picking Phaeocystidibacter luteus G18,2g yeast powder, 1L seawater) in liquid nutrient medium test tube, 37 DEG C, 24h cultivated by 200rpm/min shaking table, inoculum size with 2% is inoculated into substratum MB (5g Zulkovsky starch, 5g Tryptones, 2g yeast powder, 1L seawater) in fermention medium, 37 DEG C, 200r/min shaking table constant temperature culture 48h, fermented liquid is centrifugal 5min under 10000g condition, and the nutrient solution supernatant of acquisition puts-20 DEG C of preservations as crude enzyme liquid, measures for diastatic activity.
Embodiment 2:Phaeocystidibacter merougensis G18 produces amylase optimal reactive temperature and thermostability thereof
1) the Tris-HCl buffer 0.5% Zulkovsky starch solution of pH8.0.Get 0.5% Zulkovsky starch solution 225 μ L and be placed in 1.5mL centrifuge tube, preheating 5min in 25 DEG C, 37 DEG C, 45 DEG C, 55 DEG C, 65 DEG C, 70 DEG C, 72 DEG C, 75 DEG C, 80 DEG C and 90 DEG C of water-baths respectively, add the crude enzyme liquid of 25 μ L embodiment 1 preparations, after accurate reaction 20min, add 450 μ L DNS solution and mix 5min in rearmounted boiling water bath, be cooled to room temperature, get 50 μ L reaction solutions in 384 orifice plates, measure 540nm place absorbancy (Eon microplate spectrophotometer, Bio Tek company of the U.S.).Often group arranges 3 Duplicate Samples, and each thermograde, to boil the crude enzyme liquid of 5min inactivation for negative control, with the enzyme activity of 70 DEG C of reaction systems for 100%, calculates this enzyme relative reactivity at each temperature and is figure (see Fig. 2).Result shows, and this enzyme shows higher relative activity (being greater than 80%) in 70 ~ 75 DEG C of reaction systems, 70 DEG C time, show maximum enzyme activity.
2) crude enzyme liquid prepared by embodiment 1 in 45 DEG C, 65 DEG C, 72 DEG C, 75 DEG C and 80 DEG C of water-baths respectively after thermal treatment 0h, 0.25h, 0.5h, 0.75h, 2h, 3h and 4h, by embodiment 2 1) described method measures enzyme activity.With undressed original enzyme liquid vigor for 100%, calculate the thermostability (see Fig. 3) of this enzyme.Result shows, thermal treatment 45min under 65 DEG C of conditions, and residue relative activity is still higher than 80%.
Embodiment 3:Phaeocystidibacter merougensis G18 produces amylase optimal reaction pH and ph stability thereof
1) with the damping fluid of different pH value, (pH 3.0 ~ 6.0 is Ning Meng Suan – sodium citrate buffer solution, 7.0 ~ 8.0 is phosphate buffered saline buffer, and 9.0 ~ 11 is Gan An Suan – sodium hydrate buffer solution) secure ph be respectively 4,6,7,7.5,8,8.5,9,9.5,10,10.5 0.5% Zulkovsky starch solution.Often group arranges 3 Duplicate Samples, and each pH gradient, to boil the crude enzyme liquid of 5min inactivation for negative control, measures amylase activity.With the enzyme activity of pH8.0 reaction system for 100%, calculate the relative reactivity of this enzyme under each pH (see Fig. 4).Result shows, and this enzyme shows higher relative activity (being greater than 80%) in the reaction system of pH8.0 ~ 9.5, in pH8.0 reaction system, show maximum enzyme activity.
2) with above-mentioned pH damping fluid and concentrated hydrochloric acid, the pH value that sodium hydroxide solution adjusts crude enzyme liquid is respectively 1.5,3.5,4.5,5.5,6.5,8.5,9.5,10.5,12 place 60min in room temperature, at pH 8.0, measures residual enzyme and live under temperature 70 C.With untreated original enzyme liquid vigor for 100%, calculate the ph stability (see Fig. 5) of this enzyme.Result shows, and this enzyme is all more stable in the scope of pH4.5 ~ 10.5, and residue relative activity is all higher than 80%.
Reference:
1. Pan's scene etc., the separation and purification of recombination high temperature-resistant α-amylase and character research thereof. National University of the Inner Mongol's journal (natural science edition), 2007 (01): p.43-47.
2. Liu Xu east, Xu Yan, a kind of separation and purification of new acid-stable α-amylase and zymologic property. application and environmental organism journal, 2008 (02): p.235-239.
3. insult house thrifty, Wang Ning, Wang Lintao, the isolation and purification of high reactivity α-amylase. University Of Suzhou's journal (medicine), 2009 (05): p.930-932.
4.Zheng,X.W.,et al.,A marine algicidal actinomycete and its active substance against the harmful algal bloom species Phaeocystis globosa.Applied Microbiology and Biotechnology,2013.97(20):p.9207-9215。
5.Zhou,Y.Y.,et al.,Phaeocystidibacter luteus gen.nov.,sp nov.,a member of the family Cryomorphaceae isolated from the marine alga Phaeocystis globosa,and emended description of Owenweeksia hongkongensis.International Journal of Systematic and Evolutionary Microbiology,2013.63:p.1143-1148。
Claims (8)
1. produce a diastatic bacterial strain Phaeocystidibacter merougensis G18, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCC No.9861.
2. bacterial strain Phaeocystidibacter merougensis G18 as claimed in claim 1 is for generation of diastatic purposes.
3. produce a diastatic method, comprising:
The bacterial strain Phaeocystidibacter merougensis G18 described in claim 1 is utilized to ferment; With the amylase extracted in fermented liquid.
4. method according to claim 3, constant temperature culture under the condition of wherein said fermentation pH 6.0 ~ 8.0,28 ~ 37 DEG C in fermentation culture.
5. method according to claim 3, wherein said fermentation culture comprises in often liter of seawater, 2 ~ 10g starch, 5 ~ 15g Tryptones and 2 ~ 5g yeast powder.
6. the amylase obtained according to the method described in claim 3 ~ 5.
7. amylase according to claim 6, wherein said diastatic reaction conditions is pH8.0 ~ 9.5, and temperature is 70 ~ 75 DEG C.
8. amylase according to claim 7, wherein said diastatic reaction conditions is pH8.0, and temperature is 70 DEG C.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112553180A (en) * | 2020-12-29 | 2021-03-26 | 自然资源部第三海洋研究所 | Archaea high-temperature amylase and application thereof |
| CN112779188A (en) * | 2021-01-19 | 2021-05-11 | 自然资源部第二海洋研究所 | Medium-temperature bacterium for producing high-salinity tolerance ester hydrolase and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US537099A (en) * | 1895-04-09 | Cement-injector for repairing pneumatic tires | ||
| CN102127514A (en) * | 2010-12-01 | 2011-07-20 | 江南大学 | Strong-stability moderate-temperature neutral alpha-amylase high-producing bacterium and zymologic property thereof |
| CN102633910A (en) * | 2012-03-28 | 2012-08-15 | 常熟市滨江农业科技有限公司 | Method for extracting pentosan from wheat by-products |
-
2014
- 2014-12-15 CN CN201410777816.5A patent/CN104560792B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US537099A (en) * | 1895-04-09 | Cement-injector for repairing pneumatic tires | ||
| CN102127514A (en) * | 2010-12-01 | 2011-07-20 | 江南大学 | Strong-stability moderate-temperature neutral alpha-amylase high-producing bacterium and zymologic property thereof |
| CN102633910A (en) * | 2012-03-28 | 2012-08-15 | 常熟市滨江农业科技有限公司 | Method for extracting pentosan from wheat by-products |
Non-Patent Citations (3)
| Title |
|---|
| BEHAL等: "Characterization of Alkaline α-Amylase from Bacillus sp. AB 04", 《INTERNATIONAL JOURNAL OF AGRICULTURE & BIOLOGY》 * |
| 刘震等: "淀粉分解菌的筛选及产酶条件的优化", 《饲料工业》 * |
| 陈明贤: "高温长杆菌（Bacillusstearothermophilus91）的分离及其所产高温淀粉酶纯化及特性之研究", 《台湾博硕士论文知识加值系统》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112553180A (en) * | 2020-12-29 | 2021-03-26 | 自然资源部第三海洋研究所 | Archaea high-temperature amylase and application thereof |
| CN112779188A (en) * | 2021-01-19 | 2021-05-11 | 自然资源部第二海洋研究所 | Medium-temperature bacterium for producing high-salinity tolerance ester hydrolase and application thereof |
| CN112779188B (en) * | 2021-01-19 | 2022-06-17 | 自然资源部第二海洋研究所 | Medium-temperature bacterium for producing high-salinity tolerance ester hydrolase and application thereof |
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