CN104545797A

CN104545797A - Ultraviolet electronic spectrum and infrared molecular spectrum combined early stage tumor detection comprehensive confirmation

Info

Publication number: CN104545797A
Application number: CN201310469451.5A
Authority: CN
Inventors: 王汉成
Original assignee: Individual
Current assignee: Individual
Priority date: 2013-10-10
Filing date: 2013-10-10
Publication date: 2015-04-29

Abstract

Molecule interiors are level structures formed by various motion states. Molecule interior motions include the electron motion, the molecular vibration and the molecular rotation; energy is quantized; and vibration-rotational level jumping is only accompanied with electronic energy level jumping, and therefore molecule band spectra are formed. Cancers refers to DNA hydrogen electron excitation motion, and stimulation jumping of the vibration energy level and the rotation energy level of cancer molecules is judged. The condition that Fourier infrared molecular spectra more abundant and more complex than electrons are used for singly diagnosing tumors is unilateral. It is a significant cause for considering that the infrared spectra are used for singly diagnosing the tumors is indecisive. It is inexperienced that the ultraviolet electronic spectra are used for detecting the early stage tumor, and the cancer ultraviolet electronic spectrum blue shift Doppler effect is characterized in that the distance between DNAs and protein becomes shorter, and the bonding force is strengthened. The cancer DNA hydrogen excited electronic state is achieved, DNA second-stage structure quantum inheritance is achieved, jumping of molecules is judged, and cancer molecule spectra are formed. The ultraviolet electronic spectra and the infrared molecular spectra are combined, strong points are learnt to offset weakness, and most comprehensive analysis and confirmation of the tumor molecules and electronic structures is facilitated.

Description

Uv electron spectrum is combined infrared molecular spectrum detection infantile tumour and is made a definite diagnosis comprehensively

Background technology

Only be made up of the electromagnetic wave of different wave length, in spectrum analysis, the light that a certain fixed star sends is divided into the light of different wave length by spectrogram, thus forms a color ribbon, and we are referred to as spectrogram.Gas in fixed star will absorb the light of some wavelength, thus will form dark Absorption Line in spectrogram.Each element can produce specific Absorption Line, and astronomer, by the Absorption Line in research spectrogram, can learn which a certain fixed star plant elementary composition by.By the position of Absorption Line in star aberration spectrogram compared with Absorption Line position same under laboratory light source (red shift, blue shift), the situation of the relative earth movements of this fixed star can be known.

Molecular Spectroscopy is in the application of biomedical aspect, and also more and more close to universe secret, and extensive use spectra methods detects life universe.In recent years, vibrational spectrum, comprises FTIR-ATR optical fiber, micro ft-ir spectroscopy, and the methods such as FT-Raman spectrum and near infrared spectrum are used to distinguish normal structure and tumor tissues, and the difference of both utilizations a series of absworption peak in spectrum differentiates.These methods have a lot of advantage, evolution process is formed by tumor, first be that gene changes, corresponding molecular structure is caused to change, further formation cancerous cell, until the formation of lump, there is occupation time process, at this moment could detect with physical methods such as nuclear magnetic resonance, NMR, C T, B ultrasonic, X-rays.And infrared and Raman spectrum can be used as the sensitive probe of molecule structure change, detect the exception of tumor cells structure, namely just likely cancer is detected at tumorigenic second stage, likely develop into a kind of method of early diagnosis of tumor, and early diagnosis is the key for the treatment of of cancer, thus develop spectrographic method cancer diagnosis significant.

Technical field

The analysis that infrared spectrum is widely used in living things system particularly tumor cell or tissue is the thing of recent two decades, by 1984, infrared spectrum is just used to normal person, lymphocyte research in disorders of blood patient hemocyte trace it to its cause is because living things system is complicated, molecular species is many, containing a large amount of chemical groups, the absorption band of these chemical groups is folded into complicated absorption band mutually, the strong absorption band of water also masks biomolecule absorption band in addition, make the infrared spectrum of living things system complicated, be difficult to resolve and application the .70 age after, microcomputer data processing, Fourier converts the development of instrument, these problems are just progressively solved.Through computer digital animation, the absworption peak of water is easy to deduct from sample, and overlapping peak can separate ..., make the application of its biological field become possibility.Infrared spectrometer really becomes the research tool of organism, is from research for human lymphocyte in 1984.The development pole the earth of Fourier transform infrared spectrometer improves signal to noise ratio, shortens Measuring Time, makes its application development swift and violent, develops into molecular biology and clinic study field by chemical field.

The biochemical characteristic of tumor shows as: cell carcinogenesis must cause cellular component as the change in content, configuration, conformation, intermolecular and intramolecular interaction etc. of protein, nucleic acid, sugar, fat and hydrone.The basic biochemical characteristics of cancerous cell is malignant proliferation, poorly differentiated, invasion and attack surrounding tissue and transfer.The biochemical foundation of malignant proliferation is about metabolic pathway such as the anabolism of nucleic acid, nucleotide, the biosynthesis of protein of synthetic cell constituent all obviously strengthens; Propagation is usually the prerequisite of differentiation, and cancer cell multiplication is faster, and it is poorer to break up, and tumor biochemistry phenotype occurs disdifferentiation phenomenon.During cell carcinogenesis, cell membrane changes on composition, 26S Proteasome Structure and Function, and as memebrane protein is lost, surface negative charge increases.The basic biochemical characteristics of cancerous cell is the infrared spectrum analysis basis of tumor.Compared with the FTIR of normal structure, the FTIR of tumor tissues also exists significant difference in the spectroscopy parameters such as spectral pattern, adsorption frequency, peak intensity.These diversityes are pointed out: cancerous tumor cell and organize amplifying nucleic acid relative amount to increase; The relative amount of glycogen or collagen declines: in nucleic acid molecules, the Hydrogen bonding forces of phosphodiester group strengthens; Lipid methylene chain becomes more unordered.The application of Fourier infrared spectrum in cancer diagnosis obtains some significant results: (1) normal cell tissue and tumor cell tissue also exist obvious diversity in the spectroscopy parameters such as FTIR spectral pattern, adsorption frequency, relative intensity, and these spectroscopy diversityes disclose their compositions on a molecular scale and molecular structure diversity.(2) results of IR and pathological diagnosis result have good dependency, and prompting infrared spectrum can with doing the malignant and benign lesion of tumor, the typing of tumor and classification.Can study Carcinogenesis Mechanism from molecule is that molecular level cancer diagnosis provides possibility in the future.

Summary of the invention

By using statistical method relative analysis, there is notable difference between the FTIR spectrum finding good, malignant tissue, wherein malignant tumor spectrum has following characteristics; (1) the obvious red shift of the amide I band of cancerous tissue (P<0.01), but there is blue shift (P<0.05) in amide II band, in cancerous tissue spectrum I1640/I1460 and I1640/I1550 comparatively benign tissue obviously raise (P<0.01), illustrate that malignant tumor not only protein structure there occurs change, the quantification of protein also has significant change.In research cancerous tissue, the feature of protein and nucleic acid molecules hydrogen bond is thought, hydrogen bond is the important function power maintaining and promote protein and nucleic acid higher structure.During canceration the change of hydrogen bond and the special biological behaviour of cancerous cell closely related, therefore likely utilize these spectral signatures of hydrogen bond as the index of breast cancer diagnosis and prediction canceration probability.(2) change of protein adsorption bands of a spectrum: after tissue canceration, the bands of a spectrum of profiling protein matter amino acid residue are subjected to displacement, and move to 1165cm by 1155cm, illustrates that its kinds of protein there occurs change, and the hydrogen bonding degree of group increases; (3) relevant to nucleic acid 1241cm-1 bands of a spectrum are obviously blue shifted to (12381.29 ± 2.87) cm-1, I1080/I1460 comparatively benign goitre tissue has rising (P<0.05), show that in cancerous tissue, in phosphodiester group, PO2 increases, the increase of cancerous cell Nucleic Acid is one of factor of bands of a spectrum enhancing.The increase of phosphorylated protein content has significant contribution to these bands of a spectrum.Result of study shows, infrared spectrum is expected to become quick diagnosis malignant tumor means.

Intramolecule is the level structure formed by various kinestate.Intramolecular motion has electron motion, molecular vibration and molecule rotation, and their energy is all quantized, therefore can form electron energy level, vibration level and rotational energy level.The electron energy level of molecule is 10 electron-volts of (eV) magnitudes, few with the energy level difference of atom; The vibration level of molecule be approximately electron energy level doubly, the rotational energy level of molecule be approximately the m/M of electron energy level doubly, wherein m is the quality of electronics, and M is the quality of Typical molecular.Larger than electron mass thousands of times to ten thousand times of quality due to Typical molecular, thus molecular vibration energy level is 0.1 electron-volt (eV), rotational energy level is 0.001eV, and intramolecular motion also should comprise electron transition campaign.Namely tumor cells shakes, turns the transition differential movement of energy level to only have the transition athleticism of Tumour DNA electron energy level to determine.Therefore molecular energy level is more complicated than the energy level of atom.With enriching than atom, the much complicated infrared molecular spectrum of Fourier checks separately and determines that between good, malignant tissue, difference is unilateral.This is the dithery major reason of infrared molecular spectrum just also.

Military large medical science virus institute personnel utilize a kind of ultra-violet absorption spectrum technology, successful probe is to HeLa cell quantum and momentum relationship, the relation of famous de Broglie wavelength that Here it is and momentum, this formula is exactly Einstein-de Broglie equation that we know together with ω=hv." biological Broglie " connects the speed of energy delivery and cancer.The group velocity demonstrating tumor proliferation ripple is equal to the wetting velocity of each fundamental particle in tumor.The research of cancerous cell aggressivity is defined than more complete oncogene physics opinion.All absworption peak such as compared with normal tissue DNA and trend towards blue-shifted phenomenon through uv electron spectral detection cancerous cell, cancer DNA, cancer RNA, oncoprotein ultra-violet absorption summit aspect.Proto-oncogene ultraviolet absorption peak wavelength is 260 millimicrons, and after Oncogene Mutation becomes oncogene, ultraviolet absorption peak wavelength changes and shows as 255 millimicrons of highly excited level materials.Wavelength move to shortwave aspect 5 millimicrons quite mutually with energy be every mole be 2-3 thousand card, (1,000 cards can make 1 g of heat being upgraded to 1000 DEG C waterborne), virus, oncogene excited state quantum, complex fastness and hard-decomposed, all depend on this.Oncogene ultraviolet absorption peak wavelength blue shift and oncogene matter wave phenomenon, the former Russian scholar reports (1948) to some extent for a long time in " nucleic acid structure and biological activity thereof ".Virus institute research worker also acquires MCF-10A people's normal mammary epithelial MCF-7 breast cancer cell MDA-MB-231 breast cancer cell ultra-violet absorption spectrum respectively and studies, detect through HeLa cell quantum and momentum relationship, antioncogene technology of preparing detects, all successfully confirm that oncogene ultra-violet absorption spectrum surveys the character that energy technology can detect Tumour DNA and protein strong bonded key, the electron excitation Transition Spectra of Tumour DNA can be used as gene barrier height-gene valve can value, is measuring of nucleus degree of stability.Affect the determiner of notable difference between good, the malignant tissue of FTIR spectrum inspection.

, there is not vibration level and rotational energy level in the electron energy level in atom.There is not vibration and rotate in isolated atom.Only have molecule (such as hydrogen molecule) just to there is electron energy level, vibration level and rotational energy level simultaneously.The energy bite of electron energy level is greater than vibration level interval, and vibration level interval is greater than rotational energy level interval.Generally speaking, when only having electron energy level generation transition, just along with the transition of vibrational-rotational level, thus molecule band spectrum can be formed.Ultra-violet absorption spectrum associating FTIR spectrum is only the most comprehensive means detecting tumor cells, electronic structure.

Detailed description of the invention

Uv electron absorption spectrum and infrared molecular absorption spectrum can independently use in the tumor classification made a definite diagnosis.By clinical multiple-case measurement collection infrared spectrum, uv electron spectroscopic data, comprise esophagus, stomach, intestinal, liver and gall capsule, kidney, lung, melanoma, the parotid gland, thyroid and mammary gland etc. and find that the infrared spectrum of tumor and normal structure is obviously different, through the diagnosing tumor data base set up the induction-arrangement com-parison and analysis of determination data.Can independently study.Once Clinical detection infantile tumour, during comprehensive confirmed cases, then Combining diagnosis should be implemented.Uv electron spectrum and infrared molecular spectrum joint-detection infantile tumour, to make a definite diagnosis comprehensively.

Uv electron spectrum combine with infrared molecular spectrum only do not detect infantile tumour disease jointly, disclose uniting of oncogene intramolecule electron motion and molecular entergy level structure especially, it is reliable canceration molecule, electronic evidence for tumor aetiology provides.

In FTIR spectrum research cancerous tissue, the feature of protein and nucleic acid molecules hydrogen bond is thought, hydrogen bond is the important function power maintaining and promote protein and nucleic acid higher structure.Canceration Hydrogen bonding forces strengthens, and amide II band but occurs that the special biological behaviour of blue shift and cancerous cell is closely related, therefore likely utilizes these spectral signatures of hydrogen bond as breast cancer diagnosis and the index predicting canceration probability.

Uv electron absorption spectrum detects cancerous cell, cancer DNA, cancer RNA, oncoprotein uv absorption electron transition summit aspect all absworption peaks such as compared with normal tissue DNA and trend towards blue-shifted phenomenon.Proto-oncogene ultraviolet absorption peak wavelength is 260 millimicrons, and after Oncogene Mutation becomes oncogene, ultraviolet absorption peak wavelength changes and shows as 255 millimicrons of highly excited level materials.Wavelength move to shortwave aspect 5 millimicrons quite mutually with energy be every mole be 2-3 thousand card, (1,000 cards can make 1 g of heat being upgraded to 1000 DEG C waterborne), virus, oncogene excited state quantum, complex fastness and hard-decomposed, all depend on this.The transition of Tumour DNA electron excitation, uv electron absorption spectrum can not conclude that this is which kind of electron transition is studied through FTIR spectrum, and in cancerous tissue, the feature of protein and nucleic acid molecules hydrogen bond is thought, canceration is that Hydrogen bonding forces strengthens, and can certainly be thus; Canceration is the transition of DNA hydrogen bond electron excitation.There is blue shift in amide II band, the closely related uv electron absorption spectrum of what special biological behaviour that FTIR spectrum research can not understand it and cancer explicitly points out, cancer blue spectral shift Doppler effect is, DNA and protein distance each other shortens, strengthens.Oncogene excited state quantum, secondary structure quantum genetic etc., affect, the excite transitions of molecule vibrational-rotational level of determining, thus form cancer molecule band spectrum.Uv electron absorption spectrum detect infantile tumour, although powerful must through at the beginning of one's career.The history that infrared spectrum tumor analysis has it long.Uv electron spectrum is combined with infrared molecular spectrum, mutually learns from other's strong points to offset one's weaknesses, and this is more conducive to tumor cells, the most comprehensive diagnostic of electronic structure and parsing.

Ultraviolet spectra is the same with infrared spectrum, can from the feature of molecule, electronic horizon announcement serum, the cleer and peaceful normal human serum of blood of patients with cancer that utilized infrared spectrum technology to research and analyse, spectrum change relatively between them, find out corresponding Changing Pattern, middle infrared optical fiber and coupling Late Cambrian tumor tissues of popping one's head in are in body in situ detection.The intestinal cancer original position detected is similar in vitro tissue spectrum at body spectrum.This innovation result shows that middle infrared optical fiber and infrared spectrum coupling may develop in a kind of surgical operation and can't harm, fast and accurately at body detecting method.When infrared spectrum generaI investigation finds with object of suspicion, then do ultraviolet spectra and infrared spectrum joint inspection, to make a definite diagnosis tumor comprehensively.A series of achievements in research that uv electron spectrum combines infrared molecular spectrum show, can differentiate good, malignant tumor quickly and accurately, likely develop into the new method making a definite diagnosis cancer in early days, fast.Be respectively the diagnosis of 90% by the accuracy rate of diagnosis of Fourier transform infrared spectroscopy FTIRS to cancerous tissue, the diagnosis of 100% can be brought up to rapidly.Uv electron spectrum and infrared molecular spectrum Combining diagnosis infantile tumour, make a definite diagnosis comprehensively.

Claims

1. a series of clinical, scientific achievement shows, uv electron spectrum combines infrared molecular spectrum can make a definite diagnosis good, malignant tumor quickly and accurately.The diagnosis of 100% can be brought up to rapidly by Fourier transform infrared spectroscopy (FTIRS) spectrum to the diagnosis of cancerous tissue 90%, make a definite diagnosis tumor comprehensively.It is the unique reliable new method that infantile tumour is made a definite diagnosis.Uv electron spectrum is combined infrared molecular spectrum diagnosis infantile tumour technology and should be protected.(uv electron spectrum and infrared molecular spectrum independent early diagnosis tumor technology, method be not within the scope of this).

2. uv electron spectrum combines infrared molecular spectrum early diagnosis tumor new method, and explain the motion of canceration DNA hydrogen electron excitation, the excite transitions of cancer molecule is shaken, rotational energy level, is the most authoritative parsing of tumor cells, electronic structure.For oncosis etiology provides uv electron excitation energy data and infrared molecular spectrum excited vibrational energy level data.Cancer uv electron blue spectral shift Doppler effect is, the Distance Shortened between DNA and protein, and adhesion strengthens.Cancer DNA hydrogen excited electronic state, DNA secondary structure quantum genetic, tumor uv electron excitation spectrum decide typing and the classification (energy bite of electron energy level is greater than molecular vibration energy level interval) of tumor, are to excite the major reason forming the infrared molecule band spectrum of cancer.Tumor uv electron excitation spectrum technical data should be protected.