CN104531724A - Gene PpRd for regulating fruit flesh cyanin synthesis and application thereof - Google Patents
Gene PpRd for regulating fruit flesh cyanin synthesis and application thereof Download PDFInfo
- Publication number
- CN104531724A CN104531724A CN201510062155.2A CN201510062155A CN104531724A CN 104531724 A CN104531724 A CN 104531724A CN 201510062155 A CN201510062155 A CN 201510062155A CN 104531724 A CN104531724 A CN 104531724A
- Authority
- CN
- China
- Prior art keywords
- gene
- pprd
- cyanin
- application
- peach
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a gene PpRd for regulating fruit flesh cyanin synthesis and application thereof, relating to plant gene engineering and biotechnology. The gene PpRd nucleic acid sequence is disclosed as SEQ ID NO.1, and the protein sequence coded by the gene PpRd is disclosed as SEQ ID NO.2. The application of the PpRd gene is implemented by (1) designing a molecular marker WPS23 according to the sequence of the PpRd gene, and applying the molecular marker WPS23 in marker auxiliary screening in breeding; and (2) application of expression protein sequence and gene functions: detecting and screening cyanidin content related phenotype according to the expression level of the gene, or enhancing or lowering the gene expression level by genetic transformation to obtain the expected flesh color character. The invention obtains a key gene for cyanin accumulation. When the PpRd gene is used for marker auxiliary selection to instruct hybridization breeding of peaches, the screening can be performed in advance, thereby saving the breeding time and enhancing the breeding efficiency.
Description
Technical field
The present invention relates to plant genetic engineering and biotechnology, particularly relate to a kind of regulate and control fruit pulp cyanin synthesis gene PpRd and application; Be specifically related to the clone of the transcription factor PpRd of regulating peach pulp cyanin synthesis, genetic analysis, functional verification and application; PpRd can regulate and control the expression of the transcription factor of other regulation and control cyanin synthesis, thus affects the accumulation of pulp cyanin; Also relate to the screening of the molecule marker of fruit tree hybridization breeding, to the application of this gene in fruit breeding.
Background technology
Peach was cultivated in China before about 4000, Europe has been passed to the Silk Road period afterwards, one of Important Economic fruit tree becoming now Temperate Region in China (W.yu-lin.Peach growing and germplasm inChina.Acta Horticulturae 173:51-5) in Rome.According to pulp colour, Peach cultivars can be divided three classes according to pulp colour: white, yellow and red.The Peach cultivars of current production uses more than 80% is plain boiled pork honey peach (Gong Linzhong, what Warburg Pincus, Wang Furong, Gu Xia, 10 parts of Hubei local red meat peach biological resources characteristics observations, Journal of Fruit Science 2008,25:413-417).And yellow peach and red meat peach in state-owned long cultivation history.Yellow peach can trace back to the Tang Dynasty (before about 1360), and in " Luoyang flowers and trees note " that when red meat peach sees the Song dynasty the earliest, Zhou Shihou compiles.At European red meat peach (Hedrick UP, the Thepeaches of New York that is recorded in 1659 the earliest.1917)。
Peach belongs to the Rosaceae, and its genome is made up of 8 karyomit(e)s, and haploid genome about 230 megabasse is to size.The genetic analysis of red meat peach has had some to report.At ' Harrow Blood ' × ' F of Rutgers RedLeaf 2n '
2in filial generation, the ratio of red meat peach and Bai Tao is 1:3, therefore infers that this red meat peach proterties is controlled (the .Inheritance of the blood-fleshtrait in peach.Hortscience1998 such as Werner DJ by a recessive gene; 33:1243-1246).This site is positioned to the top of the 4th linkage group.But, have recently and be reported in that Chinese red meat peach ' May is fresh ' is inner has found a new red meat peach proterties, be considered to by one be positioned at No. five chromosomal dominant genes control (the .Characterization and genetic mapping of a new blood-flesh traitcontrolled by the single dominant locus DBF in peach.Tree Genetics andGenomes2013 such as Shen Z; 9:1435-1446).In addition, in the filial generation of mountain peach P1908 and introduced variety ' Summergrand ', 2 quantitative trait locus QTL laying respectively at No. 1 linkage group and No. 3 linkage groups are found (the .QTL analysis of quality traits in an advancedbackcross between Prunus persica cultivars and the wi ld relative speciesP.davidiana.Theoretical and Appl ied Genetics2004 such as Quilot B; 109:884-897).These all illustrate that red meat proterties is complicated, may by multiple Gene Handling.
The red meat proterties of peach is because the cyanin accumulation of high-content causes.Cyanin plays an important role in development of plants, and such as protective plant is from the injury of intense light irradiation, and opposing disease, attracts zoochory pollen or seed.Cyanin is also of great advantage to HUMAN HEALTH, is therefore regarded as an important indicator of fruit quality.The precursor of cyanin is anthocyanidin, is not yet to carry out glycosylation, methylate or acetylizad material.Anthocyanidin is how many according to the hydroxy position of B ring and number, mainly can be divided into pelargonin, Minor centaury florigen, delphinidin.In peach meat, main cyanin composition is Cy-3-G, also has Cyanidin-3-rutinoside .Fruit quality of Redhaven andRoyal Glory peach cultivars on seven different rootstocks.Journalof Agricultural and Food Chemistry 201159:9394-9401 such as () Orazem P of trace in addition.
The biosynthesis pathway of cyanin after deliberation very clear in plant, research in the last few years mainly concentrates in its transcriptional control level.MYB, basic helix-loop-helix (bHLH) and WD40 tri-kinds of transcription factors form MBW transcription complex and are attached in the promotor of the structure gene of Phenylpropanoid Glycosides, flavonoid and cyanin anabolism path, activate transcribing of genes involved.Except MBW complex body, also have some other transcription factor or modulin to be in the news and participate in the synthesis of cyanin.
In peach, anthocyanidin has had some to report in exocarp, endocarp, blade and the Accumulation Mechanism in spending.Report is had to show the epidermis of peach and the painted of nearly core place relevant with being positioned at three myb transcription factors that No. three karyomit(e) connects .Regulation of anthocyanin biosynthesis inpeach fruits.Planta2014DOI 10.1007/s00425-014-2078-2 such as () Rahim MA recently.But cause the key gene of these Traits change still unknown.Myb gene just may participate in concrete " executive " that the synthesis of pulp cyanin regulates, but is not the basic reason causing genetic diversity.And cross-breeding needs the locus of distinct key variation, therefore MYB class transcription factor still cannot be applied to the genetic breeding of peach pulp colour proterties.Our research work identifies this key gene, can be directly used in cross-breeding, for the enrichment of peach pulp good character provides theory and technology support.
Summary of the invention
Object of the present invention be just to provide a kind of regulate and control the gene PpRd of fruit pulp cyanin synthesis and application thereof, specifically:
The object of the present invention is achieved like this:
One, the gene PpRd of fruit pulp cyanin synthesis is regulated and controled
The present invention is red meat peach " Radix Campylotropis Hirtella (Herba Myrsines Africanae) " kind and hybrid Population thereof specific to Hubei Province, QTL (Inheritance of Quantitative Characters) location is carried out to peach pulp acid Anthocyanin Content, pass through genetic method, by the data of peach genome frame diagram, be separated and obtain the gene that can determine peach pulp Anthocyanin Content, i.e. aforesaid peach PpRd gene, its sequence is as shown in SEQ ID NO.1, and coded protein sequence is as shown in SEQ ID NO.2.
Specifically: determine that Radix Campylotropis Hirtella (Herba Myrsines Africanae) is coloured to a large amount of accumulation because being fructescence cyanin by HPLC (high performance liquid chromatography); By carrying out QTL location to ' Radix Campylotropis Hirtella (Herba Myrsines Africanae) ' × ' dawn ' colony; Red meat proterties is positioned the interval that the fifth pair of chromosomes top is about 200kb by success; By RNA-seq comparing, find most possible candidate gene ppa022238m (called after PpRd); Then according to its core series of variation design molecule marker WPS23, analyze in progeny population and find that WPS23 associates completely with red meat proterties; Carry out tobacco transient transformation assay simultaneously, confirm that PpRd gene can make tobacco leaf redden under the cooperation of specific gene, thus confirm the gene be separated to.
Two, the application of the gene PpRd of fruit pulp cyanin synthesis is regulated and controled
1, according to its sequences Design molecule marker of PpRd gene WPS23 (its primer sequence sees attached list 1), for the mark assisting sifting in breeding, pulp colour and anthocyanidin content can be screened in advance;
According to the simple repeated sequence SSR molecular marker around this locus, design primer carries out polyacrylamide gel PAGE strip analysis; Find that 3 SSR marker have separation in dawn, and in Radix Campylotropis Hirtella (Herba Myrsines Africanae), only have 1 mark WPS23 to have separation; Analyze in progeny population and find that WPS23 associates completely with red meat proterties; Therefore this evaluation of markers can be used in the cross-breeding of Radix Campylotropis Hirtella (Herba Myrsines Africanae).
2, the application of its expressing protein sequence and gene function, detect screening anthocyanidin content Relevant phenotype according to the expression amount of this gene or the sequence hypotype of transcript, or improved or reduce this gene expression amount by transgenic method to obtain the pulp colour proterties expected.
According to the splicing result of Radix Campylotropis Hirtella (Herba Myrsines Africanae) pulp RNA degree of depth sequence rna-Seq, and homogenic sequence, determine the transcript sequence of PpRd; By Peach cultivars, ' whole genome sequence of lovel l ', determines the gene structure of Radix Campylotropis Hirtella (Herba Myrsines Africanae) PpRd; According to its transcript sequence design primer, pcr amplification PpRd total length, is connected to expression vector, during overexpression vector cotransformation tobacco with the PpNAC1 of auxiliary character, the blade of tobacco can be made to redden in one week in post-conversion, the cyanin of accumulation more amount.Carry out virus induced gene silencing experiment (VIGS, virus induced gene si lencing) in Radix Campylotropis Hirtella (Herba Myrsines Africanae) mature fruit and prove that fruit redness goes down to some extent, and control group does not find this phenomenon when in after PpRd is silenced one week.Above-mentionedly prove from the pros and cons, PpRd gene directly can determine redness and the Anthocyanin Content of pulp, thus can be used for the breeding of Peach fruits quality.
Compared with the existing methods, the present invention has following advantages and positively effect:
1, present invention obtains the upstream gene that a cyanin accumulation regulates, and be different from the myb transcription factor serial genes found in former research, and confirm that PpRd is in MYB upstream functionating;
2, the present invention utilizes RNA-Seq to splice result ligand because of framing figure and obtains full length gene, with traditional degenerated primer PCR or RACE method than more fast and reliable, all there is very large uncertainty in degenerate pcr or RACE method, and the method for direct predicted gene from reference genome does not have the support of experimental data due to it, the mRNA whether necessary being of prediction cannot be known, the gene clone method that we adopt is very easy to realize, and still has good feasibility in the future;
3, the key gene PpRd that the control pulp that finds of the present invention is painted, for marker assisted selection, instructs the cross-breeding of peach, can screen in advance, saves breeding time, improves breeding efficiency.
Accompanying drawing explanation
Fig. 1 be red meat Peach cultivars ' Radix Campylotropis Hirtella (Herba Myrsines Africanae) ' and plain boiled pork kind ' Bai Feng ' fruit development time interim Anthocyanin Content variation diagram,
An A-two kind pulp colour is with the change in fruit development period;
B and C-high-performance liquid chromatogram determination standard model and spend latter 95 days fruit Anthocyanin Content;
After DAFB-spend how many days.
Fig. 2 is the variation diagram of expression amount with fruit development of realtime fluorescent quantitative PCR experiment gained cyanin metabolic pathway structure gene,
The expression amount of black bar-' Radix Campylotropis Hirtella (Herba Myrsines Africanae) ', the expression amount of white columns-' Bai Feng ',
X-coordinate-fruit development period (after spending how many days),
Ordinate zou-this gene is relative to the expression amount of reference gene (PpTEF2).
Fig. 3 is the sibship figure of MYB and bHLH,
A gene PpMYB10.1 of A-peach and peach other the MYB10 of cyanin and other species may be regulated to regulate the systematic evolution tree of the myb gene of cyanin metabolism,
Two bHLH of B-peach and other regulate the systematic evolution tree of the bHLH gene of flavonoid metabolism.
Fig. 4 is the expression amount variation diagram of real-time fluorescence quantitative PCR disclose peach MYB and bHLH relevant to cyanin metabolism phase during fruit development,
The expression amount of black bar-' Radix Campylotropis Hirtella (Herba Myrsines Africanae) ',
The expression amount of white columns-' Bai Feng ',
X-coordinate-fruit development period (after spending how many days),
Ordinate zou-this gene is relative to the expression amount of reference gene (PpTEF2).
Fig. 5 is Dual-luciferase reportor systerm and tobacco leaf transient expression result figure, can promote synthesis and the accumulation of cyanin when proving PpMYB10.1 and PpbHLH3 coexpression.
A-use Dual-luciferase reportor systerm is to the research of the structure gene PpDFR and PpUFGT of two in cyanin pathways metabolism, interpretation of result uses one-way analysis of variance (LSD, P<0.01), a/b/c represents different statistical packet;
B-carry out PpMYB10.1 and PpbHLH3 mixing coexpression on cultivation tobacco leaf.There is erythema to occur, illustrate that cyanin is by a large amount of Induced synthesis.
Fig. 6 is that the QTL using the hybrid Population of ' Radix Campylotropis Hirtella (Herba Myrsines Africanae) ' × ' dawn ' to carry out red meat proterties locates schematic diagram, and red meat proterties QTL is positioned peach the fifth pair of chromosomes top,
Mark in diagram is SSR molecular marker, uses the maternal linkage group (P1) of " two false test cross " the Theory Construction and male parent linkage group (P2).
The expression amount schematic diagram (real-time fluorescence PCR analytical results) of Fig. 7 candidate gene in the offspring of the hybrid Population of ' Radix Campylotropis Hirtella (Herba Myrsines Africanae) ' × ' dawn ',
X-coordinate-different offspring's individual plant,
Ordinate zou represents the expression amount of this gene relative to reference gene (PpTEF2);
Fig. 8 is the distribution plan of the SSR molecular marker of candidate gene PpRd locus,
Black box-exon, the position mark of 6 SSR marker is in figure, and final WPS21, WPS22 and WPS23 have polymorphism in male parent ' dawn ', and only has WPS23 to have polymorphism in maternal ' Radix Campylotropis Hirtella (Herba Myrsines Africanae) '.The pulp colour complete linkage of WPS23 and filial generation.
Fig. 9 is the Dual-Luciferase system of Ben Shi tobacco leaf and the instantaneous colour induction experimental result schematic diagram of cultivation tobacco leaf, and checking PpRd and PpNAC1 coexpression can promote the expression of cyanin regulatory gene PpMYB10.1;
In Dual-Luciferase system, PpRd and five candidate co-activater coexpression, finding the expression that can promote PpMYB10.1 when PpRd and PpNAC1 coexpression, in instantaneous colour induction experiment, demonstrating PpRd and PpNAC1 to promoting that the expression of PpMYB10.1 all plays an important role.
The result schematic diagram of Figure 10 yeast two-hybrid and Photinus pyralis LUC complementary system, proves that PpRd and PpNAC1 does in vivo mutually.
By PpRd and PpNAC1 connection segment on the carrier of yeast two-hybrid; Yeast two-hybrid uses positive and negative two kinds of modes to verify; Photinus pyralis LUC complementary system uses zero load to compare, and experimental group PpNAC1-NLuc+CLuc-Red detects very strong uciferase activity, and control group does not almost detect uciferase activity.
Figure 11 is that VIGS system verification PpRd accumulates to peach pulp the schematic diagram that cyanin is necessity in a large number,
Use ' Radix Campylotropis Hirtella (Herba Myrsines Africanae) ' fruit of moderate maturation, carry out VIGS and infect; Respectively two of PpRd sections of nucleotide sequences are connected in pTRV2 carrier; Find after one week that experimental group pulp colour obviously goes down, and control group (empty carrier) is substantially unchanged.
Embodiment
Embodiment 1: the abstraction and quantification of cyanin.
Get the peach Meat Sample of about 1 gram or the tobacco sample of 0.1 gram, grind with liquid nitrogen, to be suspended in the hydrochloric acid methanol (hydrochloric acid volume fraction is 0.1%) of 5 milliliters of precoolings 24 hours.Centrifugal 10 minutes of 3000g, collects supernatant liquor, on a rotary evaporator 30 degrees Celsius of evaporated in vacuo.The deionized water (1.18 millimolar concentration HCl) of residue acidifying is resuspended, and transfers in pretreated CNWBOND LC-C18SPE pillar.The cyanin methanol-eluted fractions of 1 milliliter, and filter with 0.22 micron membrane filter, loading carries out high performance liquid chromatography detection.
Embodiment 2: real-time fluorescence quantitative PCR detects the relative content of gene transcripts
The sample of about 0.2 gram uses liquid nitrogen to grind for extracting RNA (ribonucleic acid).RNA extracts the plant polyphenol polysaccharide total RNA extraction reagent box using Beijing Zhuan Meng company.Sepharose with 1.2% detects the quality of RNA.RNA concentration is detected with NanoDrop instrument.The synthesis of the first chain cDNA uses PrimeScriptReverse Transcriptase test kit (precious biological).Real-time fluorescence quantitative PCR system is 20 microlitres, comprises the cDNA of about 100 nanograms, 2 × SYBR premixEx Taq of 0.2 micromolar every bar primer and 10 microlitres
tM(precious biological).Amplification program is as follows: 95 DEG C of denaturations of 30 seconds, is the amplifications (95 DEG C 5 seconds, 60 DEG C 34 seconds) of 40 circulations afterwards.The multiple Kong Sanci of each amplification.The gene TEF2 of peach is as internal reference.
Expression amount uses typical curve standard measure.
The list of primers that table 1 detects for real-time fluorescence quantitative PCR
Embodiment 3: Dual-luciferase reportor systerm detects transcription factor to the activation capability of promotor
Young tender Ben Shi tobacco leaf is as injection material.By the Agrobacterium (GV3101) of the transcription factor vector that drives containing 35S or the plant promoter carrier containing pGreen 0800, in the LB substratum containing Rifampin and respective carrier resistance, 28 DEG C of activation shake bacterium to OD
600nmvalue is about 0.8, collected by centrifugation thalline, is resuspended in balance and infects liquid (10 mmole MgCl to room temperature
2, and 100 micromole's Syringylethanone acetosyringone) in.Use 1 milliliter aseptic go needle applicator inject mycetome infect liquid to Ben Shi Tobacco Leaves.Uciferase activity is detected after 2-3 days.Use test kit is DLAR-2 test kit (TargetingSystems, USA).
Embodiment 4: the instantaneous colour induction experiment of tobacco leaf proves that PpMYB10.1 has the function of induction cyanin
Activated by the Agrobacterium (GV3101) of the carrier of MYB10.1, bHLH3, bHLH33 and GUS of driving containing 35S promoter, method is with embodiment 3.Infect and prepare with embodiment 3.Cultivation tobacco young leaflet tablet is used to be acceptor material.Within 7 days, observe afterwards and take pictures.
Embodiment 5: the exploitation of the fifth pair of chromosomes SSR molecular marker
According to the peach gene order-checking data announced, use misa software prediction No. five chromosomal SSR sites, use primer 5.0 to design primer.PCR program is as follows: 94 DEG C of denaturations 3 minutes, after connect 35 circulations, circulate interior 94 DEG C of sex change 30 seconds, and 57 DEG C of annealing 30 seconds, extends 30 seconds, be circulated throughout rear extension eventually 5 minutes, final product is stored in 4 DEG C of refrigerators.Before PAGE glue electrophoresis, PCR primer is placed on ice after 10 minutes immediately 95 degrees Celsius of sex change, and adding sample-loading buffer can run glue by point sample.PAGE glue preparation, electrophoresis and silver staining method are as follows: 1. making sheet.Size two boards liquid detergent is cleaned and dries, then wipe one time with dehydrated alcohol, dry.Non-affine plate adds 2 milliliters of dehydrated alcohols and 1 milliliter of stripping silane, wipes even, dry.Affine plate adds 2mL alcohol and the affine silane of 35 μ L, wipes even, dry.2. join glue.Every block glue gets 12 to 13 milliliter of 4% acrylamide, 65 to the urea-tbe buffer liquid of 70mL, the ammonium persulphate of 10% of 600 microlitres and the N of 60 microlitres, N, N', N'-Tetramethyl Ethylene Diamine (TEMED) shakes up, glue is poured between the plate clipped, does not form bubble.After good, comb anti-plug to be entered in glue about 0.5 centimetre dark, leave standstill 2-5 hour.
3. loading.Comb is taken out, scraping off broken glue, plate is put on electrophoresis apparatus, with damping fluid (1 × TBE) to not having non-affine plate, being plugged wire, 80 watts of power electrophoresis preheating 15 minutes.Plate will be mixed with the PCR primer point-to-point sample hole of the sex change of sample-loading buffer, 80 watts of power electrophoresis 1 to 2 hours.
The SSR molecular marker list of primers that table 2 is developed
4. silver dye.By the proportional arrangement stop bath of every premium on currency 1 gram of Silver Nitrate.The affine plate after electrophoresis and non-affine plate separately, fixing 15 minutes are soaked in stop bath after affine plate is soaked several minutes in distilled water.Developing solution is made in the ratio of every premium on currency 20 grams of sodium hydroxide, 0.4 gram of sodium carbonate and 4 milliliters of formaldehyde.Flat board being put into developing solution and soaks 3-5 minute, occurring to there being banding pattern.Flat board is put into distilled water to soak 1 minute, taking-up is dried.
Embodiment 6: yeast two-hybrid checking PpRd and PpNAC1 interactions between protein
Rd will be connected with
1-179, Rd
1-313, NAC1
1-193, NAC1
1-262and NAC1
1-285pGBKT7 carrier and empty carrier proceed in yeast strain Y2HGold, NAC1 total length and Rd will be connected with
1-313pGADT7 carrier and empty carrier proceed in yeast strain Y187.After hybridization, SD-Trp-Leu, SD-Trp-Leu-Ade-His+AbA and SD-Trp-Leu-Ade-His+AbA+X-α-Gal solid medium is rule.Take pictures after 3-7 days.
Embodiment 7: Photinus pyralis LUC complementary system checking PpRd and PpNAC1 interactions between protein.By the Agrobacterium (GV3101) activation (method is with embodiment 3) containing NLuc, CLuc, PpNAC1-CLuc and NLuc-Rd carrier.Infect and prepare with embodiment 3.Use the young leaflet tablet of Ben Shi cigarette as acceptor.Inject after 3 days, detect LUC active, the test kit of use is
luciferase Assay System (Promega company).
Embodiment 8: use VIGS method induction Rd gene silencing at Radix Campylotropis Hirtella (Herba Myrsines Africanae) pulp
Get close to full ripe Radix Campylotropis Hirtella (Herba Myrsines Africanae) pulp as acceptor, adopt TRV1-TRV2 system as instrument.The TRV2 carrier and empty TRV1 carrier that are connected with Rd are transformed into GV3101 bacterial strain respectively, prepare with embodiment 3 before activation and conversion.The injection of bacterium liquid is carried out with the syringe of 1 milliliter of aseptic needle-less on the fresh Radix Campylotropis Hirtella (Herba Myrsines Africanae) fruit surface gathered.Fruit after injection is positioned in the incubator of 21 DEG C, low-light 16h/8h illumination/dark cycle.Cut fruit after one week and observe proterties, take pictures and collect pulp material preservation in-80 DEG C of refrigerators.
Claims (2)
1. regulate and control a gene PpRd for fruit pulp cyanin synthesis, it is characterized in that:
Its sequence is as shown in SEQ ID NO.1, and coded protein sequence is as shown in SEQ ID NO.2.
2., by the application regulating and controlling the PpRd gene that fruit pulp cyanin is synthesized described in claim 1, it is characterized in that:
1. according to its sequences Design molecule marker of PpRd gene WPS23, for the mark assisting sifting in breeding, pulp colour and anthocyanidin content can be screened in advance;
Described primer sequence is as shown in SEQ ID NO.3 and SEQ ID NO.4;
2. the application of its expressing protein sequence and gene function, detect screening anthocyanidin content Relevant phenotype according to the expression amount of this gene or the sequence hypotype of transcript, or improved or reduce this gene expression amount by transgenic method to obtain the pulp colour proterties expected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510062155.2A CN104531724B (en) | 2015-02-06 | 2015-02-06 | Regulate and control gene PpRd and its application of fruit pulp anthocyanin synthesis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510062155.2A CN104531724B (en) | 2015-02-06 | 2015-02-06 | Regulate and control gene PpRd and its application of fruit pulp anthocyanin synthesis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104531724A true CN104531724A (en) | 2015-04-22 |
| CN104531724B CN104531724B (en) | 2017-09-26 |
Family
ID=52847350
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510062155.2A Active CN104531724B (en) | 2015-02-06 | 2015-02-06 | Regulate and control gene PpRd and its application of fruit pulp anthocyanin synthesis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104531724B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105837669A (en) * | 2016-05-05 | 2016-08-10 | 山东农业大学 | Anthocyanin regulatory protein MsARF3 obtained from functional apples and encoding gene and application of protein |
| CN105925721A (en) * | 2016-07-09 | 2016-09-07 | 中国农业科学院郑州果树研究所 | Single nucleotide polymorphism marker site, primer and kit for identifying coloring property of peach epidermis and application |
| CN108289429A (en) * | 2015-10-30 | 2018-07-17 | 瑞克斯旺种苗集团公司 | Generate the tomato plants of the fruit containing beneficial compound |
| CN112695046A (en) * | 2020-12-25 | 2021-04-23 | 浙江大学 | Chrysanthemum anthocyanin transport gene CmGST1 and application thereof |
| CN113265411A (en) * | 2021-05-28 | 2021-08-17 | 中国农业科学院郑州果树研究所 | Gene for controlling peach blossom/fruit peel color and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103993019A (en) * | 2014-04-30 | 2014-08-20 | 华中农业大学 | Application of rosa rugosa RrDFR1 gene to regulate and control synthesis of plant anthocyanidin |
-
2015
- 2015-02-06 CN CN201510062155.2A patent/CN104531724B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103993019A (en) * | 2014-04-30 | 2014-08-20 | 华中农业大学 | Application of rosa rugosa RrDFR1 gene to regulate and control synthesis of plant anthocyanidin |
Non-Patent Citations (4)
| Title |
|---|
| RAUL PIRONA ET AL.: "Fine mapping and identification of a candidate gene for a major locus controlling maturity date in peach.", 《BMC PLANT BIOLOGY》 * |
| TIZIANA CRIFÒ ET AL.: "Short cold storage enhances the anthocyanin contents and level of transcripts related to their biosynthesis in blood oranges", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 * |
| VERDE I ET AL.: "Uniprot ID:M5WDM1_PRUPE", 《UNIPROT》 * |
| 俞明亮等: "红肉桃研究与利用进展", 《果树学报》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108289429A (en) * | 2015-10-30 | 2018-07-17 | 瑞克斯旺种苗集团公司 | Generate the tomato plants of the fruit containing beneficial compound |
| CN105837669A (en) * | 2016-05-05 | 2016-08-10 | 山东农业大学 | Anthocyanin regulatory protein MsARF3 obtained from functional apples and encoding gene and application of protein |
| CN105837669B (en) * | 2016-05-05 | 2019-02-12 | 山东农业大学 | Obtained from the anthocyanin modulin MsARF3 and its encoding gene of functional form apple and application |
| CN105925721A (en) * | 2016-07-09 | 2016-09-07 | 中国农业科学院郑州果树研究所 | Single nucleotide polymorphism marker site, primer and kit for identifying coloring property of peach epidermis and application |
| CN105925721B (en) * | 2016-07-09 | 2019-05-24 | 中国农业科学院郑州果树研究所 | For identifying single nucleotide polymorphism site, primer, kit and the application of Peach fruits epidermis coloring character |
| CN112695046A (en) * | 2020-12-25 | 2021-04-23 | 浙江大学 | Chrysanthemum anthocyanin transport gene CmGST1 and application thereof |
| CN113265411A (en) * | 2021-05-28 | 2021-08-17 | 中国农业科学院郑州果树研究所 | Gene for controlling peach blossom/fruit peel color and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104531724B (en) | 2017-09-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Huang et al. | Subfunctionalization of the Ruby2–Ruby1 gene cluster during the domestication of citrus | |
| Plavcová et al. | Gene expression patterns underlying changes in xylem structure and function in response to increased nitrogen availability in hybrid poplar | |
| Hatlestad et al. | The beet Y locus encodes an anthocyanin MYB-like protein that activates the betalain red pigment pathway | |
| Zhu et al. | Natural variations in the MYB transcription factor MYB31 determine the evolution of extremely pungent peppers | |
| Wang et al. | Control of grain size, shape and quality by OsSPL16 in rice | |
| Alakonya et al. | Interspecific RNA interference of SHOOT MERISTEMLESS-like disrupts Cuscuta pentagona plant parasitism | |
| Hattori et al. | The ethylene response factors SNORKEL1 and SNORKEL2 allow rice to adapt to deep water | |
| Shatil‐Cohen et al. | Bundle‐sheath cell regulation of xylem‐mesophyll water transport via aquaporins under drought stress: a target of xylem‐borne ABA? | |
| Romano et al. | AtMYB61, an R2R3‐MYB transcription factor, functions as a pleiotropic regulator via a small gene network | |
| Zheng et al. | Anthocyanin profile and gene expression in berry skin of two red Vitis vinifera grape cultivars that are sunlight dependent versus sunlight independent | |
| CN104531724A (en) | Gene PpRd for regulating fruit flesh cyanin synthesis and application thereof | |
| Ren et al. | Localization shift of a sugar transporter contributes to phloem unloading in sweet watermelons | |
| US20160355835A1 (en) | Methods of modulating plant seed and nectary content | |
| Manavella et al. | Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies | |
| Whitehill et al. | Histology and cell wall biochemistry of stone cells in the physical defence of conifers against insects | |
| Li et al. | A rapid and efficient transient expression system for gene function and subcellular localization studies in the tea plant (Camellia sinensis) leaves | |
| Lu et al. | A GmRAV ortholog is involved in photoperiod and sucrose control of flowering time in soybean | |
| Zhang et al. | Mesophyll cells’ ability to maintain potassium is correlated with drought tolerance in tea (Camellia sinensis) | |
| CN107400715B (en) | Development and application of special molecular marker and probe of decaploid elytrigia elongata | |
| Gao et al. | The promoter structure differentiation of a MYB transcription factor RLC1 causes red leaf coloration in empire red leaf cotton under light | |
| Yang et al. | Overexpression of PvWOX3a in switchgrass promotes stem development and increases plant height | |
| Qi et al. | The PavNAC56 transcription factor positively regulates fruit ripening and softening in sweet cherry (Prunus avium) | |
| Lei et al. | A chalcone synthase controls the verticillium disease resistance response in both Arabidopsis thaliana and cotton | |
| Dong et al. | The regulatory role of gibberellin related genes DKGA2ox1 and MIR171f_3 in persimmon dwarfism | |
| Portillo et al. | Isolation of RNA from laser‐capture‐microdissected giant cells at early differentiation stages suitable for differential transcriptome analysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |