CN104530251A - Antioxidant compound polysaccharide and preparation method thereof - Google Patents
Antioxidant compound polysaccharide and preparation method thereof Download PDFInfo
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Abstract
The invention relates to antioxidant compound polysaccharide. The antioxidant compound polysaccharide is composed of the following components in parts by weight: 600-900 parts of armillaria luteo-virens polysaccharide, 300-500 parts of lycium ruthenicum polysaccharide, 300-500 parts of nitraria tangutorum polysaccharide and 50-200 parts of lycium barbarum polysaccharide. Meanwhile, the invention further provides a preparation method of the compound polysaccharide. The compound polysaccharide prepared in the invention has antioxidation, can be widely used in such industries as food and health products, and meanwhile can also be widely used in various antioxidant fields to serve as an intermediate.
Description
Technical field
The present invention relates to a kind of polysaccharide product and preparation method thereof, particularly relate to a kind of anti-oxidant complex polysaccharide and preparation method thereof.
Background technology
Human body, when with extraneous contact, comprises the factors such as breathing, outside contamination, diet are unhealthy, Exposure to Sunlight, pressure in human body, constantly produce free radical, and biological cells and tissues can decompose by free radical, affects metabolic function, and can cause different health problems.Scientific research shows, cancer, aging, arteriosclerosis, the disease such as cardiovascular are all relevant with free radical.Therefore, the natural antioxidants of supplemented with exogenous is one of approach delaying or reduce human body oxidative damage, resist aging.
Armillaria luteo-virens (Amillarialuteo-vriens), has another name called yellow mushroom, belongs to Basidiomycotina, Hymenomycetes, dried mushroom order, Tricholomataceae, Armillaria.Armillaria luteo-virens contains and enriches polysaccharide, protein, amino acid etc., has enhancing body immunologic function, Tumor suppression growth and the effect such as anti-oxidant, has higher nutritive value and food therapy health effect.
Black fruit lyceum (
lycium ruthenicum Murr.) be Gobi Desert Terrain area mainly build one of mass-planting thing, strong stress resistance, drought-resistant saline and alkaline, germinating power is strong, is excellent shrub of checking winds and fixing drifting sand, and is purple when its berry is ripe, nontoxic.Black fruit lyceum fruit has stomach invigorating tonifying spleen, nourishing Yin and moistening lung, effect such as to help digestion of invigorating blood circulation.Modern scientific research proves that lycium ruthenicum fruit has anti-oxidant, antifatigue, anti-hypoxia, reducing blood-fat, prevents and treats the effects such as arteriosclerosis.
Zygophyllaceae (
zygophyllceae) white thorn (
nitraria), be Gobi Desert Terrain area mainly build one of mass-planting thing, strong stress resistance, drought-resistant saline and alkaline, germinating power is strong, is excellent shrub of checking winds and fixing drifting sand, and is red-purple when its berry is ripe, nontoxic.Fructus Nitraria schoberi has stomach invigorating tonifying spleen, nourishing Yin and moistening lung, effect such as to help digestion of invigorating blood circulation.Modern scientific research proves that berry of nitraria tangutorum bobr has anti-oxidant, antifatigue, anti-hypoxia, reducing blood-fat, prevents and treats the effects such as arteriosclerosis.
Matrimony vine is the shrub plant of Solanaceae Lycium, and matrimony vine has and reduces blood sugar, anti-fatty liveranti-fatty liver, the effect such as anti-oxidant.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of anti-oxidant complex polysaccharide of excellent property.
Another technical problem to be solved by this invention is to provide the preparation method of this anti-oxidant complex polysaccharide.
For solving the problem, the anti-oxidant complex polysaccharide of one of the present invention, it is characterized in that: it is made up of the component of following weight parts: Armillaria luteo-virens polysaccharide 600 ~ 900 parts, lycium ruthenicum polysaccharide 300 ~ 500 parts, white thorn polysaccharide 300 ~ 500 parts, lycium barbarum polysaccharide 50 ~ 200 parts.
A kind of anti-oxidant complex polysaccharide, is characterized in that: it is made up of the component of following weight parts: Armillaria luteo-virens polysaccharide 700 ~ 800 parts, lycium ruthenicum polysaccharide 350 ~ 450 parts, white thorn polysaccharide 350 ~ 450 parts, lycium barbarum polysaccharide 100 ~ 150 parts.
The preparation method of a kind of anti-oxidant complex polysaccharide as above, comprises the following steps:
(1) prepare Armillaria luteo-virens Crude polysaccharides:
Armillaria luteo-virens is added water according to the ratio of liquid ratio 5g:1mL ~ 50g:1mL, then adopt microwave loss mechanisms microwave power be 300w ~ 800w, Extracting temperature is 40 ~ 90 DEG C, extraction time is 2 times, extract under the condition of each 1 ~ 3h, obtain extracting solution A, after this extracting solution A is concentrated into paste through 40 ~ 90 DEG C, add dehydrated alcohol by 4 times of described extracting solution A volume again to precipitate, be precipitated thing A; Described throw out A is dried to constant weight at 50 ~ 90 DEG C, obtains Armillaria luteo-virens Crude polysaccharides;
(2) prepare black fruit lyceum Crude polysaccharides:
Black fruit lyceum is added water according to the ratio of liquid ratio 5g:1mL ~ 50g:1mL, then adopt microwave loss mechanisms microwave power be 300w ~ 800w, Extracting temperature is 30 ~ 90 DEG C, extraction time is 2 times, extract under the condition of each 1 ~ 3h, obtain extracting solution B, after this extracting solution B is concentrated into paste through 40 ~ 90 DEG C, add dehydrated alcohol by 4 times of described extracting solution B volume again to precipitate, be precipitated thing B; Described sediment B is dried to constant weight at 50 ~ 90 DEG C, obtains black fruit lyceum Crude polysaccharides;
(3) prepare and sting Crude polysaccharides in vain:
The ratio of stinging in vain according to liquid ratio 5g:1mL ~ 50g:1mL is added water, then adopt ultrasonic extraction ultrasonic power be 300w ~ 800w, Extracting temperature is 30 ~ 90 DEG C, extraction time is 2 times, extract under the condition of each 1 ~ 3h, obtain extracting solution C, after this extracting solution C is concentrated into paste through 40 ~ 90 DEG C, add dehydrated alcohol by 4 times of described extracting solution C volume again to precipitate, be precipitated thing C; Described throw out C is dried to constant weight at 50 ~ 90 DEG C, i.e. get Bai Ci Crude polysaccharides;
(4) prepare matrimony vine Crude polysaccharides:
Matrimony vine is added water according to the ratio of liquid ratio 5g:1mL ~ 50g:1mL, then adopt ultrasonic extraction ultrasonic power be 300w ~ 800w, Extracting temperature is 40 ~ 90 DEG C, extraction time is 2 times, extract under the condition of each 1 ~ 3h, obtain extracting solution D, after this extracting solution D is concentrated into paste through 40 ~ 90 DEG C, add dehydrated alcohol by 4 times of described extracting solution D volume again to precipitate, be precipitated thing D; Described sediment D is dried to constant weight at 50 ~ 90 DEG C, obtains matrimony vine Crude polysaccharides;
Respectively by described Armillaria luteo-virens Crude polysaccharides, black fruit lyceum Crude polysaccharides, in vain sting Crude polysaccharides, matrimony vine Crude polysaccharides adopt ceramic membrane carry out separation and purification removal of impurities, after being dried to constant weight at 50 ~ 90 DEG C, obtaining Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide respectively, sting polysaccharide, lycium barbarum polysaccharide in vain;
By described Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide, sting polysaccharide in vain, lycium barbarum polysaccharide mixes by proportioning.
Described step (5) in the aperture of ceramic membrane be 0.01 μm ~ 10 μm, its operating pressure is 0.01MPa ~ 3MPa.
A kind of anti-oxidant complex polysaccharide as above is preparing the application in healthcare products, food or medicine, it is characterized in that: this anti-oxidant complex polysaccharide makes tablet, granule, capsule, oral liquid, syrup or pill according to a conventional method.
The present invention compared with prior art has the following advantages:
1, the present invention adopts microwave extraction technology and ultrasound assisted extraction technique to Armillaria luteo-virens, black fruit lyceum respectively, stings in vain, lycium barbarum polysaccharide extracts, and has extraction time short, the advantage such as extraction efficiency is high, pollution is few.
2, because raw material of the present invention itself has antioxidant property, therefore, the complex polysaccharide extracted also has antioxygenation, can be widely used in the industry such as food, healthcare products, also can be used as intermediate simultaneously and is widely used in anti-oxidant every field.
3, each polysaccharide extracted the present invention and complex polysaccharide adopt the resistance of oxidation that DPPH method measures the clearance rate of polysaccharide, ORAC ORAC(represents polysaccharide), result shows:
The IC of the removing DPPH of Armillaria luteo-virens polysaccharide
50for 0.28mg/ml, the IC of the removing DPPH of lycium ruthenicum polysaccharide
50for 0.16mg/ml, the IC of the removing DPPH of white thorn polysaccharide
50for 0.20mg/ml, the IC of the removing DPPH of lycium barbarum polysaccharide
50for 0.18mg/ml.Armillaria luteo-virens polysaccharide ORAC is 1760 μm of ole TE/gram.Lycium ruthenicum polysaccharide ORAC is 4730 μm of ole TE/gram.White thorn polysaccharide ORAC is 4420 μm of ole TE/gram.Lycium barbarum polysaccharide ORAC is 3680 μm of ole TE/gram.Complex polysaccharide product removes the IC of DPPH
50<0.24mg/ml, complex polysaccharide ORAC are >2000 μm of ole TE/gram, complex polysaccharide excellent property.
Embodiment
embodiment 1a kind of anti-oxidant complex polysaccharide, it is made up of the component of following weight parts (g): Armillaria luteo-virens polysaccharide 600 parts, lycium ruthenicum polysaccharide 300 parts, white thorn polysaccharide 300 parts, lycium barbarum polysaccharide 50 parts.
The preparation method of this anti-oxidant complex polysaccharide, comprises the following steps:
(1) prepare Armillaria luteo-virens Crude polysaccharides:
Armillaria luteo-virens is added water according to the ratio of liquid ratio 5g:1mL, then adopt microwave loss mechanisms microwave power be 300w, Extracting temperature is 40 DEG C, extraction time is 2 times, extract under the condition of each 1h, obtain extracting solution A, after this extracting solution A is concentrated into paste through 40 DEG C, add dehydrated alcohol by 4 times of extracting solution A volume again to precipitate, be precipitated thing A; Throw out A is dried to constant weight at 50 DEG C, obtains Armillaria luteo-virens Crude polysaccharides;
(2) prepare black fruit lyceum Crude polysaccharides:
Black fruit lyceum is added water according to the ratio of liquid ratio 5g:1mL, then adopt microwave loss mechanisms microwave power be 300w, Extracting temperature is 40 DEG C, extraction time is 2 times, extract under the condition of each 1h, obtain extracting solution B, after this extracting solution B is concentrated into paste through 40 DEG C, add dehydrated alcohol by 4 times of extracting solution B volume again to precipitate, be precipitated thing B; Sediment B is dried to constant weight at 50 DEG C, obtains black fruit lyceum Crude polysaccharides;
(3) prepare and sting Crude polysaccharides in vain:
The ratio of stinging in vain according to liquid ratio 5g:1mL is added water, then adopt ultrasonic extraction ultrasonic power be 300w, Extracting temperature is 40 DEG C, extraction time is 2 times, extract under the condition of each 1h, obtain extracting solution C, after this extracting solution C is concentrated into paste through 40 DEG C, add dehydrated alcohol by 4 times of extracting solution C volume again to precipitate, be precipitated thing C; Throw out C is dried to constant weight at 50 DEG C, i.e. get Bai Ci Crude polysaccharides;
(4) prepare matrimony vine Crude polysaccharides:
Matrimony vine is added water according to the ratio of liquid ratio 5g:1mL, then adopt ultrasonic extraction ultrasonic power be 300w, Extracting temperature is 40 DEG C, extraction time is 2 times, extract under the condition of each 1h, obtain extracting solution D, after this extracting solution D is concentrated into paste through 40 DEG C, add dehydrated alcohol by 4 times of extracting solution D volume again to precipitate, be precipitated thing D; Sediment D is dried to constant weight at 50 DEG C, obtains matrimony vine Crude polysaccharides;
Respectively by Armillaria luteo-virens Crude polysaccharides, black fruit lyceum Crude polysaccharides, sting Crude polysaccharides in vain, matrimony vine Crude polysaccharides adopts aperture to be the ceramic membrane of 0.01 μm, be carry out separation and purification removal of impurities under the condition of 0.01MPa in operating pressure, after being dried to constant weight at 50 DEG C, obtaining Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide respectively, sting polysaccharide, lycium barbarum polysaccharide in vain;
By Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide, sting polysaccharide in vain, lycium barbarum polysaccharide mixes by proportioning.
embodiment 2a kind of anti-oxidant complex polysaccharide, it is made up of the component of following weight parts (g): Armillaria luteo-virens polysaccharide 900 parts, lycium ruthenicum polysaccharide 500 parts, white thorn polysaccharide 500 parts, lycium barbarum polysaccharide 200 parts.
The preparation method of this anti-oxidant complex polysaccharide, comprises the following steps:
(1) prepare Armillaria luteo-virens Crude polysaccharides:
Armillaria luteo-virens is added water according to the ratio of liquid ratio 50g:1mL, then adopt microwave loss mechanisms microwave power be 800w, Extracting temperature is 90 DEG C, extraction time is 2 times, extract under the condition of each 3h, obtain extracting solution A, after this extracting solution A is concentrated into paste through 90 DEG C, add dehydrated alcohol by 4 times of extracting solution A volume again to precipitate, be precipitated thing A; Throw out A is dried to constant weight at 90 DEG C, obtains Armillaria luteo-virens Crude polysaccharides;
(2) prepare black fruit lyceum Crude polysaccharides:
Black fruit lyceum is added water according to the ratio of liquid ratio 50g:1mL, then adopt microwave loss mechanisms microwave power be 800w, Extracting temperature is 90 DEG C, extraction time is 2 times, extract under the condition of each 3h, obtain extracting solution B, after this extracting solution B is concentrated into paste through 90 DEG C, add dehydrated alcohol by 4 times of extracting solution B volume again to precipitate, be precipitated thing B; Sediment B is dried to constant weight at 90 DEG C, obtains black fruit lyceum Crude polysaccharides;
(3) prepare and sting Crude polysaccharides in vain:
The ratio of stinging in vain according to liquid ratio 50g:1mL is added water, then adopt ultrasonic extraction ultrasonic power be 800w, Extracting temperature is 90 DEG C, extraction time is 2 times, extract under the condition of each 3h, obtain extracting solution C, after this extracting solution C is concentrated into paste through 90 DEG C, add dehydrated alcohol by 4 times of extracting solution C volume again to precipitate, be precipitated thing C; Throw out C is dried to constant weight at 90 DEG C, i.e. get Bai Ci Crude polysaccharides;
(4) prepare matrimony vine Crude polysaccharides:
Matrimony vine is added water according to the ratio of liquid ratio 50g:1mL, then adopt ultrasonic extraction ultrasonic power be 800w, Extracting temperature is 90 DEG C, extraction time is 2 times, extract under the condition of each 3h, obtain extracting solution D, after this extracting solution D is concentrated into paste through 90 DEG C, add dehydrated alcohol by 4 times of extracting solution D volume again to precipitate, be precipitated thing D; Sediment D is dried to constant weight at 90 DEG C, obtains matrimony vine Crude polysaccharides;
Respectively by Armillaria luteo-virens Crude polysaccharides, black fruit lyceum Crude polysaccharides, sting Crude polysaccharides in vain, matrimony vine Crude polysaccharides adopts aperture to be the ceramic membrane of 10 μm, be carry out separation and purification removal of impurities under the condition of 3MPa in operating pressure, after being dried to constant weight at 90 DEG C, obtaining Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide respectively, sting polysaccharide, lycium barbarum polysaccharide in vain;
By Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide, sting polysaccharide in vain, lycium barbarum polysaccharide mixes by proportioning.
embodiment 3a kind of anti-oxidant complex polysaccharide, it is made up of the component of following weight parts (g): Armillaria luteo-virens polysaccharide 700 parts, lycium ruthenicum polysaccharide 350 parts, white thorn polysaccharide 350 parts, lycium barbarum polysaccharide 100 parts.
The preparation method of this anti-oxidant complex polysaccharide, comprises the following steps:
(1) prepare Armillaria luteo-virens Crude polysaccharides:
Armillaria luteo-virens is added water according to the ratio of liquid ratio 20g:1mL, then adopt microwave loss mechanisms microwave power be 400w, Extracting temperature is 60 DEG C, extraction time is 2 times, extract under the condition of each 2h, obtain extracting solution A, after this extracting solution A is concentrated into paste through 80 DEG C, add dehydrated alcohol by 4 times of extracting solution A volume again to precipitate, be precipitated thing A; Throw out A is dried to constant weight at 70 DEG C, obtains Armillaria luteo-virens Crude polysaccharides;
(2) prepare black fruit lyceum Crude polysaccharides:
Black fruit lyceum is added water according to the ratio of liquid ratio 40g:1mL, then adopt microwave loss mechanisms microwave power be 500w, Extracting temperature is 40 DEG C, extraction time is 2 times, extract under the condition of each 2h, obtain extracting solution B, after this extracting solution B is concentrated into paste through 70 DEG C, add dehydrated alcohol by 4 times of extracting solution B volume again to precipitate, be precipitated thing B; Sediment B is dried to constant weight at 60 DEG C, obtains black fruit lyceum Crude polysaccharides;
(3) prepare and sting Crude polysaccharides in vain:
The ratio of stinging in vain according to liquid ratio 20g:1mL is added water, then adopt ultrasonic extraction ultrasonic power be 600w, Extracting temperature is 30 DEG C, extraction time is 2 times, extract under the condition of each 1.5h, obtain extracting solution C, after this extracting solution C is concentrated into paste through 70 DEG C, add dehydrated alcohol by 4 times of extracting solution C volume again to precipitate, be precipitated thing C; Throw out C is dried to constant weight at 85 DEG C, i.e. get Bai Ci Crude polysaccharides;
(4) prepare matrimony vine Crude polysaccharides:
Matrimony vine is added water according to the ratio of liquid ratio 25g:1mL, then adopt ultrasonic extraction ultrasonic power be 800w, Extracting temperature is 90 DEG C, extraction time is 2 times, extract under the condition of each 2.5h, obtain extracting solution D, after this extracting solution D is concentrated into paste through 64 DEG C, add dehydrated alcohol by 4 times of extracting solution D volume again to precipitate, be precipitated thing D; Sediment D is dried to constant weight at 65 DEG C, obtains matrimony vine Crude polysaccharides;
Respectively by Armillaria luteo-virens Crude polysaccharides, black fruit lyceum Crude polysaccharides, sting Crude polysaccharides in vain, matrimony vine Crude polysaccharides adopts aperture to be the ceramic membrane of 5 μm, be carry out separation and purification removal of impurities under the condition of 0.5MPa in operating pressure, after being dried to constant weight at 80 DEG C, obtaining Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide respectively, sting polysaccharide, lycium barbarum polysaccharide in vain;
By Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide, sting polysaccharide in vain, lycium barbarum polysaccharide mixes by proportioning.
embodiment 4a kind of anti-oxidant complex polysaccharide, it is made up of the component of following weight parts (g): Armillaria luteo-virens polysaccharide 800 parts, lycium ruthenicum polysaccharide 450 parts, white thorn polysaccharide 450 parts, lycium barbarum polysaccharide 150 parts.
The preparation method of this anti-oxidant complex polysaccharide, comprises the following steps:
(1) prepare Armillaria luteo-virens Crude polysaccharides:
Armillaria luteo-virens is added water according to the ratio of liquid ratio 35g:1mL, then adopt microwave loss mechanisms microwave power be 650w, Extracting temperature is 55 DEG C, extraction time is 2 times, extract under the condition of each 2.5h, obtain extracting solution A, after this extracting solution A is concentrated into paste through 55 DEG C, add dehydrated alcohol by 4 times of extracting solution A volume again to precipitate, be precipitated thing A; Throw out A is dried to constant weight at 70 DEG C, obtains Armillaria luteo-virens Crude polysaccharides;
(2) prepare black fruit lyceum Crude polysaccharides:
Black fruit lyceum is added water according to the ratio of liquid ratio 30g:1mL, then adopt microwave loss mechanisms microwave power be 560w, Extracting temperature is 30 DEG C, extraction time is 2 times, extract under the condition of each 2h, obtain extracting solution B, after this extracting solution B is concentrated into paste through 48 DEG C, add dehydrated alcohol by 4 times of extracting solution B volume again to precipitate, be precipitated thing B; Sediment B is dried to constant weight at 75 DEG C, obtains black fruit lyceum Crude polysaccharides;
(3) prepare and sting Crude polysaccharides in vain:
The ratio of stinging in vain according to liquid ratio 20g:1mL is added water, then adopt ultrasonic extraction ultrasonic power be 320w, Extracting temperature is 65 DEG C, extraction time is 2 times, extract under the condition of each 1.5h, obtain extracting solution C, after this extracting solution C is concentrated into paste through 65 DEG C, add dehydrated alcohol by 4 times of extracting solution C volume again to precipitate, be precipitated thing C; Throw out C is dried to constant weight at 60 DEG C, i.e. get Bai Ci Crude polysaccharides;
(4) prepare matrimony vine Crude polysaccharides:
Matrimony vine is added water according to the ratio of liquid ratio 17g:1mL, then adopt ultrasonic extraction ultrasonic power be 750w, Extracting temperature is 70 DEG C, extraction time is 2 times, extract under the condition of each 3h, obtain extracting solution D, after this extracting solution D is concentrated into paste through 65 DEG C, add dehydrated alcohol by 4 times of extracting solution D volume again to precipitate, be precipitated thing D; Sediment D is dried to constant weight at 86 DEG C, obtains matrimony vine Crude polysaccharides;
Respectively by Armillaria luteo-virens Crude polysaccharides, black fruit lyceum Crude polysaccharides, sting Crude polysaccharides in vain, matrimony vine Crude polysaccharides adopts aperture to be the ceramic membrane of 2 μm, be carry out separation and purification removal of impurities under the condition of 0.3MPa in operating pressure, after being dried to constant weight at 90 DEG C, obtaining Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide respectively, sting polysaccharide, lycium barbarum polysaccharide in vain;
By Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide, sting polysaccharide in vain, lycium barbarum polysaccharide mixes by proportioning.
embodiment 5a kind of anti-oxidant complex polysaccharide, it is made up of the component of following weight parts (g): Armillaria luteo-virens polysaccharide 750 parts, lycium ruthenicum polysaccharide 400 parts, white thorn polysaccharide 400 parts, lycium barbarum polysaccharide 80 parts.
The preparation method of this anti-oxidant complex polysaccharide, comprises the following steps:
(1) prepare Armillaria luteo-virens Crude polysaccharides:
Armillaria luteo-virens is added water according to the ratio of liquid ratio 44g:1mL, then adopt microwave loss mechanisms microwave power be 350w, Extracting temperature is 40 DEG C, extraction time is 2 times, extract under the condition of each 2.5h, obtain extracting solution A, after this extracting solution A is concentrated into paste through 70 DEG C, add dehydrated alcohol by 4 times of extracting solution A volume again to precipitate, be precipitated thing A; Throw out A is dried to constant weight at 60 DEG C, obtains Armillaria luteo-virens Crude polysaccharides;
(2) prepare black fruit lyceum Crude polysaccharides:
Black fruit lyceum is added water according to the ratio of liquid ratio 24g:1mL, then adopt microwave loss mechanisms microwave power be 750w, Extracting temperature is 54 DEG C, extraction time is 2 times, extract under the condition of each 1.8h, obtain extracting solution B, after this extracting solution B is concentrated into paste through 55 DEG C, add dehydrated alcohol by 4 times of extracting solution B volume again to precipitate, be precipitated thing B; Sediment B is dried to constant weight at 70 DEG C, obtains black fruit lyceum Crude polysaccharides;
(3) prepare and sting Crude polysaccharides in vain:
The ratio of stinging in vain according to liquid ratio 30g:1mL is added water, then adopt ultrasonic extraction ultrasonic power be 500w, Extracting temperature is 40 DEG C, extraction time is 2 times, extract under the condition of each 2.6h, obtain extracting solution C, after this extracting solution C is concentrated into paste through 70 DEG C, add dehydrated alcohol by 4 times of extracting solution C volume again to precipitate, be precipitated thing C; Throw out C is dried to constant weight at 65 DEG C, i.e. get Bai Ci Crude polysaccharides;
(4) prepare matrimony vine Crude polysaccharides:
Matrimony vine is added water according to the ratio of liquid ratio 15g:1mL, then adopt ultrasonic extraction ultrasonic power be 500w, Extracting temperature is 60 DEG C, extraction time is 2 times, extract under the condition of each 2h, obtain extracting solution D, after this extracting solution D is concentrated into paste through 70 DEG C, add dehydrated alcohol by 4 times of extracting solution D volume again to precipitate, be precipitated thing D; Sediment D is dried to constant weight at 55 DEG C, obtains matrimony vine Crude polysaccharides;
Respectively by Armillaria luteo-virens Crude polysaccharides, black fruit lyceum Crude polysaccharides, sting Crude polysaccharides in vain, matrimony vine Crude polysaccharides adopts aperture to be the ceramic membrane of 5 μm, be carry out separation and purification removal of impurities under the condition of 2MPa in operating pressure, after being dried to constant weight at 85 DEG C, obtaining Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide respectively, sting polysaccharide, lycium barbarum polysaccharide in vain;
By Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide, sting polysaccharide in vain, lycium barbarum polysaccharide mixes by proportioning.
embodiment 6a kind of anti-oxidant complex polysaccharide, it is made up of the component of following weight parts (g): Armillaria luteo-virens polysaccharide 850 parts, lycium ruthenicum polysaccharide 380 parts, white thorn polysaccharide 420 parts, lycium barbarum polysaccharide 120 parts.
The preparation method of this anti-oxidant complex polysaccharide, comprises the following steps:
(1) prepare Armillaria luteo-virens Crude polysaccharides:
Armillaria luteo-virens is added water according to the ratio of liquid ratio 30g:1mL, then adopt microwave loss mechanisms microwave power be 500w, Extracting temperature is 60 DEG C, extraction time is 2 times, extract under the condition of each 2.5h, obtain extracting solution A, after this extracting solution A is concentrated into paste through 65 DEG C, add dehydrated alcohol by 4 times of extracting solution A volume again to precipitate, be precipitated thing A; Throw out A is dried to constant weight at 70 DEG C, obtains Armillaria luteo-virens Crude polysaccharides;
(2) prepare black fruit lyceum Crude polysaccharides:
Black fruit lyceum is added water according to the ratio of liquid ratio 20g:1mL, then adopt microwave loss mechanisms microwave power be 400w, Extracting temperature is 40 DEG C, extraction time is 2 times, extract under the condition of each 3h, obtain extracting solution B, after this extracting solution B is concentrated into paste through 65 DEG C, add dehydrated alcohol by 4 times of extracting solution B volume again to precipitate, be precipitated thing B; Sediment B is dried to constant weight at 75 DEG C, obtains black fruit lyceum Crude polysaccharides;
(3) prepare and sting Crude polysaccharides in vain:
The ratio of stinging in vain according to liquid ratio 30g:1mL is added water, then adopt ultrasonic extraction ultrasonic power be 750w, Extracting temperature is 85 DEG C, extraction time is 2 times, extract under the condition of each 1.5h, obtain extracting solution C, after this extracting solution C is concentrated into paste through 45 DEG C, add dehydrated alcohol by 4 times of extracting solution C volume again to precipitate, be precipitated thing C; Throw out C is dried to constant weight at 55 DEG C, i.e. get Bai Ci Crude polysaccharides;
(4) prepare matrimony vine Crude polysaccharides:
Matrimony vine is added water according to the ratio of liquid ratio 15g:1mL, then adopt ultrasonic extraction ultrasonic power be 540w, Extracting temperature is 62 DEG C, extraction time is 2 times, extract under the condition of each 2.4h, obtain extracting solution D, after this extracting solution D is concentrated into paste through 66 DEG C, add dehydrated alcohol by 4 times of extracting solution D volume again to precipitate, be precipitated thing D; Sediment D is dried to constant weight at 55 DEG C, obtains matrimony vine Crude polysaccharides;
Respectively by Armillaria luteo-virens Crude polysaccharides, black fruit lyceum Crude polysaccharides, sting Crude polysaccharides in vain, matrimony vine Crude polysaccharides adopts aperture to be the ceramic membrane of 0.05 μm, be carry out separation and purification removal of impurities under the condition of 1MPa in operating pressure, after being dried to constant weight at 80 DEG C, obtaining Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide respectively, sting polysaccharide, lycium barbarum polysaccharide in vain;
By Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide, sting polysaccharide in vain, lycium barbarum polysaccharide mixes by proportioning.
Above-mentioned
embodiment 1 ~ 6the anti-oxidant complex polysaccharide of gained refers to preparing the application in healthcare products, food or medicine: this anti-oxidant complex polysaccharide makes tablet, granule, capsule, oral liquid, syrup or pill according to a conventional method.
Claims (5)
1. an anti-oxidant complex polysaccharide, is characterized in that: it is made up of the component of following weight parts: Armillaria luteo-virens polysaccharide 600 ~ 900 parts, lycium ruthenicum polysaccharide 300 ~ 500 parts, white thorn polysaccharide 300 ~ 500 parts, lycium barbarum polysaccharide 50 ~ 200 parts.
2. an anti-oxidant complex polysaccharide, is characterized in that: it is made up of the component of following weight parts: Armillaria luteo-virens polysaccharide 700 ~ 800 parts, lycium ruthenicum polysaccharide 350 ~ 450 parts, white thorn polysaccharide 350 ~ 450 parts, lycium barbarum polysaccharide 100 ~ 150 parts.
3. the preparation method of a kind of anti-oxidant complex polysaccharide as claimed in claim 1 or 2, comprises the following steps:
(1) prepare Armillaria luteo-virens Crude polysaccharides:
Armillaria luteo-virens is added water according to the ratio of liquid ratio 5g:1mL ~ 50g:1mL, then adopt microwave loss mechanisms microwave power be 300w ~ 800w, Extracting temperature is 40 ~ 90 DEG C, extraction time is 2 times, extract under the condition of each 1 ~ 3h, obtain extracting solution A, after this extracting solution A is concentrated into paste through 40 ~ 90 DEG C, add dehydrated alcohol by 4 times of described extracting solution A volume again to precipitate, be precipitated thing A; Described throw out A is dried to constant weight at 50 ~ 90 DEG C, obtains Armillaria luteo-virens Crude polysaccharides;
(2) prepare black fruit lyceum Crude polysaccharides:
Black fruit lyceum is added water according to the ratio of liquid ratio 5g:1mL ~ 50g:1mL, then adopt microwave loss mechanisms microwave power be 300w ~ 800w, Extracting temperature is 30 ~ 90 DEG C, extraction time is 2 times, extract under the condition of each 1 ~ 3h, obtain extracting solution B, after this extracting solution B is concentrated into paste through 40 ~ 90 DEG C, add dehydrated alcohol by 4 times of described extracting solution B volume again to precipitate, be precipitated thing B; Described sediment B is dried to constant weight at 50 ~ 90 DEG C, obtains black fruit lyceum Crude polysaccharides;
(3) prepare and sting Crude polysaccharides in vain:
The ratio of stinging in vain according to liquid ratio 5g:1mL ~ 50g:1mL is added water, then adopt ultrasonic extraction ultrasonic power be 300w ~ 800w, Extracting temperature is 30 ~ 90 DEG C, extraction time is 2 times, extract under the condition of each 1 ~ 3h, obtain extracting solution C, after this extracting solution C is concentrated into paste through 40 ~ 90 DEG C, add dehydrated alcohol by 4 times of described extracting solution C volume again to precipitate, be precipitated thing C; Described throw out C is dried to constant weight at 50 ~ 90 DEG C, i.e. get Bai Ci Crude polysaccharides;
(4) prepare matrimony vine Crude polysaccharides:
Matrimony vine is added water according to the ratio of liquid ratio 5g:1mL ~ 50g:1mL, then adopt ultrasonic extraction ultrasonic power be 300w ~ 800w, Extracting temperature is 40 ~ 90 DEG C, extraction time is 2 times, extract under the condition of each 1 ~ 3h, obtain extracting solution D, after this extracting solution D is concentrated into paste through 40 ~ 90 DEG C, add dehydrated alcohol by 4 times of described extracting solution D volume again to precipitate, be precipitated thing D; Described sediment D is dried to constant weight at 50 ~ 90 DEG C, obtains matrimony vine Crude polysaccharides;
Respectively by described Armillaria luteo-virens Crude polysaccharides, black fruit lyceum Crude polysaccharides, in vain sting Crude polysaccharides, matrimony vine Crude polysaccharides adopt ceramic membrane carry out separation and purification removal of impurities, after being dried to constant weight at 50 ~ 90 DEG C, obtaining Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide respectively, sting polysaccharide, lycium barbarum polysaccharide in vain;
By described Armillaria luteo-virens polysaccharide, lycium ruthenicum polysaccharide, sting polysaccharide in vain, lycium barbarum polysaccharide mixes by proportioning.
4. the preparation method of a kind of anti-oxidant complex polysaccharide as claimed in claim 3, is characterized in that: described step (5) in the aperture of ceramic membrane be 0.01 μm ~ 10 μm, its operating pressure is 0.01MPa ~ 3MPa.
5. a kind of anti-oxidant complex polysaccharide as claimed in claim 1 or 2 is preparing the application in healthcare products, food or medicine, it is characterized in that: this anti-oxidant complex polysaccharide makes tablet, granule, capsule, oral liquid, syrup or pill according to a conventional method.
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