CN104521899A - Novel cultivation and real-time dynamic observation device for caenorhabditis elegans - Google Patents
Novel cultivation and real-time dynamic observation device for caenorhabditis elegans Download PDFInfo
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- CN104521899A CN104521899A CN201310365192.1A CN201310365192A CN104521899A CN 104521899 A CN104521899 A CN 104521899A CN 201310365192 A CN201310365192 A CN 201310365192A CN 104521899 A CN104521899 A CN 104521899A
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- casing
- light source
- caenorhabditis elegans
- observation
- observation device
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention relates to a novel cultivation and real-time dynamic observation device for caenorhabditis elegans. Six optical cameras are arranged at the top end of an observation box, and the motion trails of the caenorhabditis elegans in a six-well cultivation plate are observed and recorded. The observation box is provided with a side wall light emitting diode lamp belt light source and a bottom plane light source, and the light sources are cooled through cooling fans and the outer side walls to maintain the stability of working temperatures of the six-well plate. The novel cultivation and real-time dynamic observation device for the caenorhabditis elegans can work in a bright field or a dark field through light source switching, and the motion trails of the caenorhabditis elegans are recorded and analyzed by a computer.
Description
Technical field
The invention belongs to Life Science Instruments field, be specifically related to a kind of novel C. Elegans Automatic Screening and cultivate and real-time dynamically observation device.
Background technology
C. Elegans Automatic Screening, as a kind of pattern nematode, has been widely used in the every field of current life scientific research, and it is also considered to study the important laboratory model of of parasitic nematode.Caenorhabditis elegans belongs to Rhabditia, Rhabditida.Take bacterium as food, its life cycle is approximately 3d, and mean lifetime is 3 weeks.It is vermiform, adult is about 1.0-1.5mm, health diameter about 70.0 μm.Caenorhabditis elegans is bilateral symmetry, and body surface has one deck cuticula covering, without merogenesis.
Existing culture of nematodes technology, mainly based on LB medium, adds CaC12, MgS04 phosphate buffer, cholesterol, and peptone and Escherichia coli make translucent medium, under 16-25 ° of C environment.Its morphological observation means are mainly the inverted microscope of observation of cell, carry out the research of nematode organ morphology aspect.But research that is long-time for nematode, grand movement form aspect is still less at present mentions.
Cell chulture observation device such as patent document " device for applying direct current observing cell biology behavior after the match " the publication number CN200810070287.X that existing document is mentioned is mainly used in shooting quiescence cells picture, and nematode volume is much larger than cell, and move about fast in culture matrix, and 6 orifice plate single hole areas are much larger than 96 orifice plates, conventional microscope limited view, therefore above-mentioned prior art cannot the motion morphology of long-time continuous track record nematode.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, by the video signal collective mainly strengthened Nematode behaviour and Monitoring System, provide a kind of have the large visual field observe nematode movement locus in 6 orifice plates cultivation observation device.Apparatus of the present invention comprise: casing, for load cultivate nematode 6 orifice plates and and scope; Power supply, for providing observation light source, scope, control appliance; Scope, for observing, recording nematode movement locus; Control appliance, for adjusting the temperature of culture environment, humidity and light source works state.
And the culture of nematodes observation device that the present invention relates to can according to observation needs, and adjustment light source and 6 orifice plate positions, carry out bright field respectively and dark field observes two kinds of mode of operations.
A kind of novel C. Elegans Automatic Screening is cultivated and real-time dynamically observation device, it comprises the observation casing 1 be made up of top board, base plate and sidewall, top board is provided with 6 optical cameras (2), be close to base plate place at the lower edge of inspection box madial wall and sidewall LED lamp strip (4) is installed, above-mentioned light bar backside heat layer is close to the madial wall observing casing (1), and the material of casing (1) is 304 stainless steels.
Hollow shadow shield (3) is metallic plate, is arranged on and observes in casing (1), be installed on the upper limb of sidewall LED lamp strip (4), and its setting height(from bottom) is basic to maintain an equal level with the height of six well culture plates (5).
Observe the plate inner surface (i.e. upper surface) of casing (1), energy supply control module (6) is installed, radiator fan (7), and base plane light source (8).Wherein base plane light source (8) is by the control of energy supply control module (6), is arranged on the below of six well culture plates (5), for above-mentioned six well culture plates provide bottom light source to throw light on.
Accompanying drawing explanation
Further illustrate structure of the present invention by reference to the accompanying drawings:
Fig. 1 is overall structure schematic diagram of the present invention.Wherein, casing 2. camera 3. hollow shadow shield 4. sidewall LED lamp strip 5. 6 well culture plate 6. energy supply control module 7. radiator fan 8. base plane light source is 1. observed.
Embodiment
See Fig. 1, a kind of novel C. Elegans Automatic Screening is cultivated and real-time dynamically observation device, it comprises by top board, the observation casing 1 that base plate and sidewall are formed, top board is provided with 6 optical cameras, openings in the ceiling diameter is 32mm, be close to base plate place at the lower edge of inspection box madial wall and sidewall LED lamp strip (4) is installed, above-mentioned light bar backside heat layer is close to the madial wall observing casing (1), light bar heat is mainly through side plate transmission, by radiator fan (7) and the external sidewall of inspection box space heat elimination towards periphery, thus keep the temperature stabilization of six well culture plates (5).
Hollow shadow shield (3) is arranged on to be observed in casing (1), is installed on the upper limb of sidewall LED lamp strip (4), and its setting height(from bottom) is basic to maintain an equal level with the height of six well culture plates (5).The upper surface of hollow shadow shield is coated with black matt coating, and lower surface is metallic reflective layer or spraying white reflection coating.Casing (1) inwall (madial wall, top board lower surface) more than hollow shadow shield (3) is all coated with black matt coating, to reduce the impact of box house light reflection on observed result.
Observe the plate inner surface (i.e. upper surface) of casing (1), energy supply control module (6) is installed, radiator fan (7), and base plane light source (8).Wherein base plane light source (8) is by the control of energy supply control module (6), is arranged on the below of six well culture plates (5), for above-mentioned culture plate provides bottom light source to throw light on.Planar light source is provided with even tabula rasa, to make six orifice plate soffit lighting uniform light.
According to the characteristic of object of observation, by controlling unlatching and the closedown of base plane light source (8), be operated in bright field and dark field duty to make this inspection box.The image that camera (2) is taken is undertaken storing and gripper path analysis by calculator.
Should be imputed, current disclosed embodiment is the illustration to all aspects, is not restrictive.Show that scope of the present invention is not according to the above description, but illustrate according to the scope of claim, comprise the meaning equal with the scope of claim and the whole change in scope.
Claims (3)
1. a novel C. Elegans Automatic Screening is cultivated and dynamic observation device in real time, it is characterized in that comprising the observation casing (1) be made up of top board, base plate and sidewall, top board is provided with 6 optical cameras (2), be close to base plate place at the lower edge of inspection box madial wall and be provided with sidewall LED lamp strip (4), above-mentioned light bar backside heat layer is close to the madial wall observing casing (1).The material of casing (1) is 304 stainless steels.
2. observation casing (1) according to claim 1, it is characterized in that, observe casing (1) inner ring and hollow shadow shield (3) is housed, hollow shadow shield (3) is metallic plate, it is installed on the upper limb of sidewall LED lamp strip (4), and its setting height(from bottom) is basic to maintain an equal level with the height of six well culture plates (5).
3. observation casing (1) according to claim 1, it is characterized in that, observe the plate inner surface (i.e. upper surface) of casing (1), energy supply control module (6) is installed, radiator fan (7), and base plane light source (8).Wherein base plane light source (8) is by the control of energy supply control module (6), is arranged on the below of six well culture plates (5), for above-mentioned six well culture plates provide bottom light source to throw light on.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310365192.1A CN104521899A (en) | 2013-08-12 | 2013-08-12 | Novel cultivation and real-time dynamic observation device for caenorhabditis elegans |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310365192.1A CN104521899A (en) | 2013-08-12 | 2013-08-12 | Novel cultivation and real-time dynamic observation device for caenorhabditis elegans |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104521899A true CN104521899A (en) | 2015-04-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310365192.1A Pending CN104521899A (en) | 2013-08-12 | 2013-08-12 | Novel cultivation and real-time dynamic observation device for caenorhabditis elegans |
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| CN (1) | CN104521899A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105300991A (en) * | 2015-10-15 | 2016-02-03 | 国家粮食局科学研究院 | Biological detection system and method for screening insecticidal active substances |
| CN107760757A (en) * | 2017-10-17 | 2018-03-06 | 上海交通大学医学院附属新华医院 | C. Elegans Automatic Screening selection of antibacterial assesses experimental provision and appraisal procedure in real time |
-
2013
- 2013-08-12 CN CN201310365192.1A patent/CN104521899A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105300991A (en) * | 2015-10-15 | 2016-02-03 | 国家粮食局科学研究院 | Biological detection system and method for screening insecticidal active substances |
| CN105300991B (en) * | 2015-10-15 | 2018-06-19 | 国家粮食局科学研究院 | A kind of biological detection system and detection method for insecticide active substance screening |
| CN107760757A (en) * | 2017-10-17 | 2018-03-06 | 上海交通大学医学院附属新华医院 | C. Elegans Automatic Screening selection of antibacterial assesses experimental provision and appraisal procedure in real time |
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Application publication date: 20150422 |