CN104509493A - Spawning device for small brown planthoppers and application method thereof - Google Patents
Spawning device for small brown planthoppers and application method thereof Download PDFInfo
- Publication number
- CN104509493A CN104509493A CN201410763567.4A CN201410763567A CN104509493A CN 104509493 A CN104509493 A CN 104509493A CN 201410763567 A CN201410763567 A CN 201410763567A CN 104509493 A CN104509493 A CN 104509493A
- Authority
- CN
- China
- Prior art keywords
- small brown
- sealed membrane
- seal
- glass tube
- brown planthoppers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 12
- 241001470017 Laodelphax striatella Species 0.000 title abstract description 6
- 235000013601 eggs Nutrition 0.000 claims abstract description 14
- 229920000742 Cotton Polymers 0.000 claims abstract description 13
- 230000009368 gene silencing by RNA Effects 0.000 claims abstract description 12
- 235000010585 Ammi visnaga Nutrition 0.000 claims abstract description 10
- 244000153158 Ammi visnaga Species 0.000 claims abstract description 10
- 239000004677 Nylon Substances 0.000 claims abstract description 10
- 239000011521 glass Substances 0.000 claims abstract description 10
- 229920001778 nylon Polymers 0.000 claims abstract description 10
- 108091030071 RNAI Proteins 0.000 claims abstract description 9
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 9
- 238000009736 wetting Methods 0.000 claims abstract description 5
- 238000007789 sealing Methods 0.000 claims abstract 5
- 239000012528 membrane Substances 0.000 claims description 22
- 241001498622 Cixius wagneri Species 0.000 claims description 18
- 235000021329 brown rice Nutrition 0.000 claims description 18
- 230000035558 fertility Effects 0.000 claims description 5
- 230000003020 moisturizing effect Effects 0.000 claims description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract description 8
- 102000040650 (ribonucleotides)n+m Human genes 0.000 abstract description 8
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 7
- 235000009566 rice Nutrition 0.000 abstract description 7
- 238000005238 degreasing Methods 0.000 abstract 3
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 239000003595 mist Substances 0.000 abstract 1
- 230000001850 reproductive effect Effects 0.000 abstract 1
- 239000007921 spray Substances 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 4
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000017448 oviposition Effects 0.000 description 2
- 238000004804 winding Methods 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 239000005394 sealing glass Substances 0.000 description 1
- 230000008684 selective degradation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a spawning device for small brown planthoppers, which is used for detecting the reproductive capacity of the small brown planthoppers in an RNA (Ribose Nucleic Acid) interference test. The device is concretely composed of: a glass tube with openings at two ends, 80-mesh nylon yarn, a rubber band, sterile toothpicks, wetting sterile degreasing cotton and a sealing film (Parafilm). During use, use the 80-mesh nylon yarn to seal one end of the glass tube; then take a toothpick wound by the wetting sterile degreasing cotton, use the sealing film to seal the degreasing cotton, and imitate rice seedlings; put in adult small brown planthoppers to lay eggs, use a layer of sealing film to seal the other end of the glass tube; add artificial feed in the center of the film, then reuse a layer of sealing film to seal the feed; after completion, use a humidifier to conduct mist spray for preserving moisture. The device can solve the problem that the small brown planthoppers do not have a spawning site in the RNAi study of a feeding method, and provides guarantee for successfully carrying out the gene function study.
Description
One, technical field
The one that the present invention relates to small brown rice planthopper is laid eggs device, during for RNA interference method research small brown rice planthopper gene function, and the detection of its fertility.
Two, background technology
Small brown rice planthopper Laodelphax striatellus Fallen is one of important rice grub of China, all has generation in all rice districts.Except affecting by temperature, agrochemical Stress Factors, fertility is the deciding factor affecting its population quantity.The function inquiring into breeding related gene can illustrate the molecular basis of small brown rice planthopper fertility change further, prevents and treats small brown rice planthopper provide target resource for utilizing biology techniques.
RNA interference (RNA interference, RNAi) refer to high conservative during evolution, brought out by double-stranded RNA, the phenomenon of the efficient selective degradation of homologous mRNA.Use RNAi technology can specific depletion or close the expression of specific gene, therefore this technology be widely used in the research of gene function.Insect RNAi mainly contains injection and feeding method, in the research of small brown rice planthopper, often take feeding method.The function of gene is determined containing the change of small brown rice planthopper phenotype after the feed of dsRNA by feeding.For most of phenotype as lethality, body colour, enzyme to be lived etc. for Biochemical changes, directly can to observe its phenotype after containing the feed of dsRNA or extract enzyme liquid feeding, then measuring that it is active.But for fertility, need to check that examination worm sprouts wings to the egg laying amount in dead whole adult stage, this just causes a contradiction.Because small brown rice planthopper is normally lay eggs in rice stem, if insert rice stem in RNAi, selection is taken food rice stem by small brown rice planthopper, instead of feed, so just cannot take in dsRNA, causes RNAi failure; And if do not insert rice stem, small brown rice planthopper will face the circumstances of laying eggs nowhere again.Therefore, we need design device of laying eggs, and make small brown rice planthopper take food artificial feed, also have the place of laying eggs simultaneously.
Three, summary of the invention
For the demand, the invention provides one and to lay eggs device, can be the small brown rice planthopper taken food containing dsRNA feed and spawning place is provided, ensure that the experiment of feeding method research gene function is smooth.
Technical scheme of the present invention is:
A kind of small brown rice planthopper lays eggs device and using method, and by the glass tube of both ends open, 80 order nylon yarn, rubber band, sterile toothpick, wetting sterile absorbent, cotton and sealed membrane (Parafilm film) forms.Concrete scheme is: the one end sealing glass tube by 80 order nylon yarn; Then get 1 sterile toothpick, be wound around with wetting sterile absorbent cotton, then with sealed membrane, absorbent cotton closed; Put into striatellus imago to be laid eggs, first close with the other end of one deck sealed membrane by glass tube, at film, central authorities add artificial feed, and then close feed with one deck sealed membrane; After completing, in the humidifier atomizing moisturizing of sealed membrane end.
The beneficial effect of the invention of the present invention is, can solve feeding method RNAi study in, small brown rice planthopper without the problem of spawning place, for the research of related gene breeding function provides safeguard.
Four, accompanying drawing explanation
The glass tube that Fig. 1 one end is closed by 80 order nylon yarn.
The toothpick schematic diagram that the moistening absorbent cotton that Fig. 2 sealed membrane is closed is wound around.
The sealed membrane of Fig. 3 different size
Fig. 4 ash flies device of laying eggs
Five, embodiment
The sealed membrane (Fig. 3) of 8 × 2.5cm is drawn thin in a longitudinal direction as far as possible, then the toothpick being wound around absorbent cotton is placed in sealed membrane central authorities, laterally folded, obtain the toothpick (Fig. 2) of the moistening absorbent cotton winding that sealed membrane is closed.With sealed membrane (Fig. 3) the sealed glass tubes other end of 2.5 × 2.5cm, at the artificial feed of sealed membrane central authorities instillation 50 microlitres containing dsRNA, then 3 × 3cm sealed membrane (Fig. 3) is used to close.Then glass tube is kept flat, take off nylon yarn, insert the toothpick of the moistening absorbent cotton of winding that sealed membrane shown in Fig. 2 is closed, again use nylon yarn closing openings, namely obtain small brown rice planthopper and to lay eggs device (Fig. 4).During RNAi experiment, take off nylon yarn, to treat that experiment adult is transferred in glass tube, again nylon yarn closing openings is used again, by sealed membrane one end towards humidifier, keep certain humidity, the toothpick (Fig. 2) that the moistening absorbent cotton that replacing in every 5 days sealed membrane is closed is wound around, on all sealed membranes that statistics changes, the quantity of ovum, can obtain the egg laying amount of experiment adult.
Claims (1)
1. a small brown rice planthopper lays eggs device and using method thereof.This device comprises the glass tube of a 12cm × 3cm, and this pipe is at the equal opening in longitudinal two ends; Seal by 80 order nylon yarn in one end of pipe, the other end double-deck sealed membrane (Parafilm film) sealing, and wrap into artificial feed between this two-layer sealed membrane; Be wound around toothpick with wetting sterile absorbent cotton again, and with sealed membrane, absorbent cotton closed; Put into striatellus imago to be laid eggs; Above-mentioned be ready to complete after, in the humidifier atomizing moisturizing of sealed membrane direction, prevent sealed membrane from chapping because of dry.This device is mainly used in the mensuration based on fertility during RNAi technique study small brown rice planthopper related gene function.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410763567.4A CN104509493A (en) | 2014-12-09 | 2014-12-09 | Spawning device for small brown planthoppers and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410763567.4A CN104509493A (en) | 2014-12-09 | 2014-12-09 | Spawning device for small brown planthoppers and application method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104509493A true CN104509493A (en) | 2015-04-15 |
Family
ID=52785806
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410763567.4A Pending CN104509493A (en) | 2014-12-09 | 2014-12-09 | Spawning device for small brown planthoppers and application method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104509493A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105794724A (en) * | 2016-03-14 | 2016-07-27 | 云南省农业科学院农业环境资源研究所 | Method for conveniently observing and feeding rice planthoppers |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101707991A (en) * | 2009-12-25 | 2010-05-19 | 浙江省农业科学院 | Artificial spawning and breeding device suitable for piercing-sucking small insects |
| CN102220343A (en) * | 2011-05-12 | 2011-10-19 | 南京农业大学 | Gene silencing technology-based lethal gene fragment Chitinase 7 of laodelphax striatellus and dsRNA (double-stranded RNA) thereof |
| CN102220341A (en) * | 2011-05-12 | 2011-10-19 | 南京农业大学 | Gene silencing technology-based lethal gene fragment Chitinase 7 of laodelphax striatellus and dsRNA (double-stranded RNA) thereof |
| CN202127723U (en) * | 2011-06-24 | 2012-02-01 | 重庆市中药研究院 | Lepidoptera insect egg connecting device |
| CN203353478U (en) * | 2013-07-23 | 2013-12-25 | 重庆市农业科学院 | Device for feeding and oviposition of imago |
| CN203555050U (en) * | 2013-10-23 | 2014-04-23 | 昆明虹之华园艺有限公司 | Device for feeding single-head thrips and observing oviposition of thrips |
| WO2014086932A1 (en) * | 2012-12-07 | 2014-06-12 | Centre Universitaire Jean Francois Champollion | Capsule comprising a liquid-tight and gas-permeable membrane, method for the production thereof, and use of same for the in vitro breeding of arthropods |
-
2014
- 2014-12-09 CN CN201410763567.4A patent/CN104509493A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101707991A (en) * | 2009-12-25 | 2010-05-19 | 浙江省农业科学院 | Artificial spawning and breeding device suitable for piercing-sucking small insects |
| CN102220343A (en) * | 2011-05-12 | 2011-10-19 | 南京农业大学 | Gene silencing technology-based lethal gene fragment Chitinase 7 of laodelphax striatellus and dsRNA (double-stranded RNA) thereof |
| CN102220341A (en) * | 2011-05-12 | 2011-10-19 | 南京农业大学 | Gene silencing technology-based lethal gene fragment Chitinase 7 of laodelphax striatellus and dsRNA (double-stranded RNA) thereof |
| CN202127723U (en) * | 2011-06-24 | 2012-02-01 | 重庆市中药研究院 | Lepidoptera insect egg connecting device |
| WO2014086932A1 (en) * | 2012-12-07 | 2014-06-12 | Centre Universitaire Jean Francois Champollion | Capsule comprising a liquid-tight and gas-permeable membrane, method for the production thereof, and use of same for the in vitro breeding of arthropods |
| CN203353478U (en) * | 2013-07-23 | 2013-12-25 | 重庆市农业科学院 | Device for feeding and oviposition of imago |
| CN203555050U (en) * | 2013-10-23 | 2014-04-23 | 昆明虹之华园艺有限公司 | Device for feeding single-head thrips and observing oviposition of thrips |
Non-Patent Citations (2)
| Title |
|---|
| 张倩 等: "灰飞虱海藻糖酶基因的克隆及RNA干扰效应", 《昆虫学报》 * |
| 陈爱玲 等: "灰飞虱体内水稻条纹病毒基因的RNA干扰效应", 《中国农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105794724A (en) * | 2016-03-14 | 2016-07-27 | 云南省农业科学院农业环境资源研究所 | Method for conveniently observing and feeding rice planthoppers |
| CN105794724B (en) * | 2016-03-14 | 2018-09-21 | 云南省农业科学院农业环境资源研究所 | A method of facilitating observation and raising planthopper |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| North et al. | Aquaporins account for variations in hydraulic conductance for metabolically active root regions of Agave deserti in wet, dry, and rewetted soil | |
| Bondada et al. | Functional xylem in the post-veraison grape berry | |
| Sicsic et al. | Microbial communities and inflammatory response in the endometrium differ between normal and metritic dairy cows at 5–10 days post-partum | |
| Ning et al. | Slit2-N inhibits PDGF-induced migration in rat airway smooth muscle cells: WASP and Arp2/3 involved | |
| Speck et al. | Humidity-dependent wound sealing in succulent leaves of Delosperma cooperi–An adaptation to seasonal drought stress | |
| CN101220377B (en) | Method for horizontal transfection of exogenesis endosymbiosis bacterium to bemisia tabaci gennadius | |
| CN103616514A (en) | Rapid diagnosis test strip of cow mastitis candida albicans | |
| Muszyńska et al. | Plasma membrane and cell wall properties of an aspen hybrid (Populus tremula× tremuloides) parenchyma cells under the influence of salt stress | |
| CN104509493A (en) | Spawning device for small brown planthoppers and application method thereof | |
| Anuracpreeda et al. | Distribution of 28.5 kDa antigen in the tegument of adult Fasciola gigantica | |
| CN101720716B (en) | Egg hatching method for culturing transgenic poultry | |
| Qian et al. | RAI14 (retinoic acid induced protein 14) is an F-actin regulator: Lesson from the testis | |
| Laue et al. | Survival, cold tolerance and seasonality of infection of E uropean horse chestnut (A esculus hippocastanum) by P seudomonas syringae pv. aesculi | |
| Ribatti et al. | The Chick Embryo Chorioallantoic Membrane as an | |
| CN203555050U (en) | Device for feeding single-head thrips and observing oviposition of thrips | |
| CN104406829A (en) | Sample making method of armyworm larva peritrophic membrane | |
| Adams et al. | Eggs under pressure: components of water potential of chameleon eggs during incubation | |
| Cavicchioli et al. | Histopathological and immunohistochemical study of exocrine and endocrine pancreatic lesions in avian influenza A experimentally infected turkeys showing evidence of pancreatic regeneration | |
| CN103063847B (en) | Preparation method for enzyme-linked immunosorbent assay (ELISA) standard substances | |
| Shepard et al. | Versican knock‐down compromises chondrogenesis in the embryonic chick limb | |
| Cappellari et al. | Reproductive biology of Teius oculatus (Sauria, Teiidae) in Southern Brazil (Dom Feliciano, Rio Grande do Sul). | |
| CN203340840U (en) | Observation device for avoid behaviors of white-striped longicorn larvae | |
| CN206251763U (en) | A kind of device that Bemisia tabaci is raised on excised leaf | |
| Chiarini‐Garcia et al. | Morphofunctional changes of female germinal epithelium to support spermatozoa along the annual reproductive cycle in an inseminating catfish (Trachelyopterus galeatus, Auchenipteridae) | |
| Wang et al. | Muscle stem cell immunostaining |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150415 |
|
| WD01 | Invention patent application deemed withdrawn after publication |