CN104502332A - Method for detecting chloride ion concentration in water - Google Patents
Method for detecting chloride ion concentration in water Download PDFInfo
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- CN104502332A CN104502332A CN201410733773.0A CN201410733773A CN104502332A CN 104502332 A CN104502332 A CN 104502332A CN 201410733773 A CN201410733773 A CN 201410733773A CN 104502332 A CN104502332 A CN 104502332A
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Abstract
The invention discloses a method for detecting chloride ion concentration in water, and particularly relates to a method for detecting chloride ion concentration in water by employing a microfluidic paper chip method. Reagents used in the method are a mercuric thiocyanate-methanol solution and a ferric nitrate-concentrated nitric acid solution. Through drawing a standard curve, a linear equation is fitted by using a proportional relationship between the chloride ion concentration and a gray value within a certain concentration range. According to the method disclosed by the invention, detection can be finished by virtue of photographic equipment and a computer; and the method is simple and convenient.
Description
Technical field
The present invention relates to a kind of method of testing of chlorine ion concentration, be specifically related to a kind of method that micro-fluidic method detects chlorine ion concentration in water.
Background technology
Micro-fluidic paper chip (paper-based microfluidics), be called for short paper chip, mainly make material using paper (as filter paper, chromatographic paper, nitrocellulose membrane etc.) as chip, in paper chip, hydrophilic and hydrophobic alternate region is formed by certain technological means, form paper passage, and be integrated with the basic operation units such as sample introduction, separation, reaction, detection, run through whole system with controlled fluid, realize a kind of chip of the analytic function of Routine Test Lab.At the beginning of 2007, Harvard University Martinez etc. have proposed this concept and since producing paper chip according to this first, micro-fluidic paper chip has been successfully applied to the fields such as medical diagnosis, environmental monitoring, food security and nutritional surveillance, and the research about it also receives increasing concern.With traditional material as organic silicon chip, organic glass etc. are compared as the stock of chip, micro-fluidic paper chip has its advantage that is unique and very attractive, as: its low, renewable, the easy process of cost, abundance, moreover, the method for making of micro-fluidic paper chip is also very simple.
About the detection of chlorion, the multiple methods such as a mole method, potentiometric titration, mercury salt titrimetry, PCl electrode method, Enrichment spectrophotometric method, the chromatography of ions are had in prior art, but these methods are not only complicated loaded down with trivial details, also often use the instruments such as such as ultraviolet spectrophotometer, cost is higher.Micro-fluidic method of testing is introduced the detection of chlorion by the present invention, not only convenient and swift, and the use of micro-fluidic paper chip also will save testing cost greatly.
Summary of the invention
The object of this invention is to provide the method for chlorine ion concentration in a kind of simple, detection water that cost is low.
The present invention is achieved by the following technical solutions:
Detect a method for chlorine ion concentration in water, comprise the following steps:
(1) the micro-fluidic paper chip required for preparation detection, the micro-fluidic paper chip required for preparation detects, described micro-fluidic paper chip comprises hydrophilic area and hydrophobic region, and described hydrophilic area is provided with some liquid inlets and a reaction solution district
(2) methanol solution and the ferric nitrate-concentrated nitric acid solution of mercuric thiocyanate is prepared;
(3) compound concentration is 0.05mg/L respectively, 0.10 mg/L, 0.1mg/L, 1.5mg/L, 2.0 mg/L, the sodium-chloride water solution of 3.0 mg/L and 5.0 mg/L;
(4) respectively by the methanol solution of described sodium-chloride water solution, mercuric thiocyanate, and ferric nitrate-concentrated nitric acid solution adds in each liquid inlet of micro-fluidic paper chip in step (1) simultaneously, wait for described micro-fluidic paper chip reaction solution district reaction 3-5 minute;
(5) after the reaction solution district colour developing in step (4), anti-to the colour developing in step (4) with camera
District is answered to take;
(6) photo of shooting in step (5) is imported in computer, by image analysis software, the picture signal in described conversion zone is converted into gray-scale value signal;
(7) utilize the gray-scale value signal in step (6) to draw chlorine ion concentration, gray value standard curve, take chlorine ion concentration as horizontal ordinate, gray-scale value is ordinate, and the linear equation that matching obtains is: y=27.4x+27.4, R
2be 0.997,
(8) with the sodium-chloride water solution in water sample replacement step (4) to be measured, repeat step (4)-(7), described in obtaining, treat that the gray-scale value of test sample brings the linear equation in step (7) into, obtain water sample concentration to be measured.
Further, the micro-fluidic paper chip in described step (1) is ordinary filter paper cake core;
Further, the method for making of the micro-fluidic paper chip in described step (1) is wax spray impact system, comprises step successively: paper chip configurations, wax spray print, baking infiltration.
Further, to be mass concentration be the concentration of methanol solution of described step (2) mercuric cyanate: 0.4%.
Further, described step (2) ferric nitrate-concentrated nitric acid solution concentration is: 0.30mol/L-5.0mol/L.
Further, described micro-fluidic paper chip surface parcel one deck hydrophobic transparent film.
Have prior art to compare, the beneficial effect that the present invention reaches is:
(1) needed for a kind of method detecting chlorine ion concentration in water provided by the invention, instrument is few, and cost is low, convenient and swift;
(2) use of micro-fluidic paper chip not only environmental protection but also cheap and easy to get, makes simple;
(3) camera, computer is utilized can to obtain the necessary data of chlorine detection ion concentration: gray-scale value, equipment needed thereby easily obtains.
(4) micro-fluidic paper chip surface parcel one deck hydrophobic transparent film can reduce the volatilization of liquid, improves accuracy of detection.
Accompanying drawing explanation
Fig. 1 is the micro-fluidic paper chip schematic diagram in the embodiment of the present invention;
Fig. 2 is the linear equation of typical curve in the embodiment of the present invention and matching.
Embodiment
Below in conjunction with accompanying drawing, the invention will be further described.Following examples only for technical scheme of the present invention is clearly described, and can not limit the scope of the invention with this.
Life produce in chlorion in water too much not only can corrosion pipeline, cause damage, and chloride ion content too much also can damage health in domestic water, the invention provides a kind of method detecting chlorine ion concentration in water simply and easily, below for detecting the method for chlorion in water in the present embodiment, step is:
(1) the micro-fluidic paper chip 1 required for preparation detection; This paper chip is ordinary filter paper type paper chip, should
Micro-fluidic paper chip comprises hydrophobic region and hydrophilic area, and described hydrophilic area is provided with some liquid inlets and a reaction solution district; Method for making is wax spray impact system, comprises paper chip configurations, the available computer graphics Software for Design of this design; Wax spray prints; Baking infiltration, baking condition is 100 DEG C, 5-10min; For subsequent use when being cooled to room temperature, in order to reduce the loss of liquid in flow process, this micro-fluidic paper chip surface parcel layer of transparent hydrophobic membrane, hydrophobic membrane is provided with the liquid inlet corresponding with liquid inlet, micro-fluidic paper chip hydrophilic area, about the more detailed step making described micro-fluidic paper chip, with reference to prior art, do not repeat them here.As shown in Figure 1, wherein 2 is mercuric thiocyanate-methanol solution injection port to micro-fluidic paper chip 1 schematic diagram in the present embodiment, and 3 is ferric nitrate-concentrated nitric acid solution injection port, and 4 is water sample injection port to be measured, and 5 is reaction solution district.
(2) prepare mass concentration be 4% mercuric thiocyanate-methanol solution, substance withdrawl syndrome be
Ferric nitrate-the concentrated nitric acid solution of 0.30mol/L-5.0mol/L, and concentration is 0.05mg/L, 0.10 mg/L, 0.1mg/L, 1.5mg/L, 2.0 mg/L, 3.0 mg/L, the sodium-chloride water solution of 5.0 mg/L; Solution preparation method is method disclosed in textbook or other documents, only briefly introduces herein:
The methanol solution of mercuric thiocyanate of the present invention is prepared by mercuric thiocyanate solute and absolute methanol solvent
Form, ferric nitrate-concentrated nitric acid solution compound method is: be first dissolved in concentrated nitric acid solution by a certain amount of iron nitrate solution, and then add a certain amount of distilled water diluting, so described ferric nitrate-red fuming nitric acid (RFNA) concentration refers to the concentration of aqueous solution of ferric nitrate and the concentration of aqueous solution of nitric acid.
(3) respectively by the methanol solution of described sodium-chloride water solution, mercuric thiocyanate, and ferric nitrate-concentrated nitric acid solution adds in each liquid inlet of micro-fluidic paper chip in step (1) simultaneously, wait for described micro-fluidic paper chip reaction solution district reaction 3-5 minute;
(4) after the reaction solution district colour developing in step (4), with camera, the reaction in step (3) is shown
Look district is taken; This place uses camera, and common shooting mobile phone or other photographing devices also can use;
(5) photo of shooting in step (4) is imported in computer, by image analysis software, the picture signal in described reaction solution district is converted into gray-scale value signal;
(6) the gray-scale value signal in step (5) is utilized to draw chlorine ion concentration, gray value standard curve, as shown in Figure 2, when experiment finds that chlorine ion concentration is 0-20mg/L, gray scale and concentration proportional, take chlorine ion concentration as horizontal ordinate, gray-scale value is ordinate, and the linear equation that matching obtains is: y=27.4x+27.4, R
2be 0.997;
(7) with the sodium-chloride water solution in water sample replacement step (4) to be measured, repeat step (4)-(6), described in obtaining, treat that the gray-scale value of test sample brings the linear equation in step (7) into, obtain water sample concentration to be measured.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the technology of the present invention principle; can also make some improvement and distortion, these improve and distortion also should be considered as protection scope of the present invention.
Claims (6)
1. detect a method for chlorine ion concentration in water, it is characterized in that, comprise the following steps:
(1) the micro-fluidic paper chip required for preparation detection, described micro-fluidic paper chip comprises hydrophilic area and hydrophobic region, and described hydrophilic area is provided with some liquid inlets and a reaction solution district;
(2) methanol solution of mercuric thiocyanate is prepared, and ferric nitrate-concentrated nitric acid solution;
(3) compound concentration is 0.05mg/L respectively, 0.10 mg/L, 0.1mg/L, 1.5mg/L, 2.0 mg/L, 3.0 mg/L, the sodium-chloride water solution of 5.0 mg/L;
(4) respectively by the methanol solution of described sodium-chloride water solution, mercuric thiocyanate, and ferric nitrate-concentrated nitric acid solution adds in each liquid inlet of micro-fluidic paper chip in step (1) simultaneously, wait for described micro-fluidic paper chip reaction solution district reaction 3-5 minute;
(5) after the reaction solution district colour developing in step (4), with camera, described reaction solution district is taken;
(6) photo of shooting in step (5) is imported in computer, by image analysis software, the picture signal in described chromogenic reaction district is converted into gray-scale value signal;
(7) utilize the gray-scale value signal in step (6) to draw chlorine ion concentration, gray value standard curve, wherein, chlorine ion concentration is horizontal ordinate, and gray-scale value is ordinate, and the linear equation that matching obtains is: y=27.4x+27.4, R
2be 0.997;
(8) with the sodium-chloride water solution in water sample replacement step (4) to be measured, repeat step (4)-(7), described in obtaining, treat that the gray-scale value of test sample brings the linear equation in step (7) into, obtain water sample concentration to be measured.
2. a kind of method detecting chlorine ion concentration in water according to claim 1, is characterized in that, the micro-fluidic paper chip in described step (1) is ordinary filter paper cake core.
3. a kind of method detecting chlorine ion concentration in water according to claim 1, it is characterized in that, the method for making of the micro-fluidic paper chip in described step (1) is wax spray impact system, comprises step successively: paper chip configurations, wax spray print, baking infiltration.
4. a kind of method detecting chlorine ion concentration in water according to claim 1, is characterized in that, to be mass concentration be described step (2) mercuric cyanate-concentration of methanol solution: 0.4%.
5. a kind of method detecting chlorine ion concentration in water according to claim 1, is characterized in that, described step (2) ferric nitrate-concentrated nitric acid solution concentration is: 0.30mol/L-5.0mol/L.
6. a kind of method detecting chlorine ion concentration in water according to claim 2, is characterized in that, described micro-fluidic paper chip surface parcel one deck hydrophobic transparent film.
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Cited By (6)
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CN105372233A (en) * | 2015-11-30 | 2016-03-02 | 华南师范大学 | Paper chip gravity / capillary flow chemiluminescence method |
CN107570244A (en) * | 2017-07-31 | 2018-01-12 | 华南理工大学 | A kind of paper substrate micro-fluidic chip of signal amplification and preparation method thereof |
CN108344848A (en) * | 2018-02-06 | 2018-07-31 | 山东五洲检测有限公司 | It is a kind of to be used to detect water quality detection agent of chlorine in water and preparation method thereof |
CN109596810A (en) * | 2018-12-25 | 2019-04-09 | 中国地质大学(北京) | A method of it simulates and detects coloured soluble component distribution in freeze-thawing process of soil |
CN112945951A (en) * | 2021-03-21 | 2021-06-11 | 西北农林科技大学 | Method for color development determination of iron distribution and content of different parts of wheat grains |
CN113655011A (en) * | 2021-10-20 | 2021-11-16 | 武汉新烽光电股份有限公司 | Microfluidic detection system and detection method thereof |
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CN103336007A (en) * | 2013-06-08 | 2013-10-02 | 中国医学科学院基础医学研究所 | Portable paper chip capable of visually detecting chlorine ion content in sweat |
CN103437240A (en) * | 2013-09-13 | 2013-12-11 | 中国科学院长春应用化学研究所 | Paper chip and preparation method thereof |
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CN102914536A (en) * | 2012-10-19 | 2013-02-06 | 大连大学 | Patterned multilayer array paper chip, preparation method and application thereof |
CN103041876A (en) * | 2012-12-27 | 2013-04-17 | 济南大学 | Preparation of electrochemical three-dimensional microfluidic paper chip with high flux, low cost and simplicity in operation, and application of electrochemical three-dimensional microfluidic paper chip to field test |
CN103336007A (en) * | 2013-06-08 | 2013-10-02 | 中国医学科学院基础医学研究所 | Portable paper chip capable of visually detecting chlorine ion content in sweat |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105372233A (en) * | 2015-11-30 | 2016-03-02 | 华南师范大学 | Paper chip gravity / capillary flow chemiluminescence method |
CN107570244A (en) * | 2017-07-31 | 2018-01-12 | 华南理工大学 | A kind of paper substrate micro-fluidic chip of signal amplification and preparation method thereof |
CN108344848A (en) * | 2018-02-06 | 2018-07-31 | 山东五洲检测有限公司 | It is a kind of to be used to detect water quality detection agent of chlorine in water and preparation method thereof |
CN109596810A (en) * | 2018-12-25 | 2019-04-09 | 中国地质大学(北京) | A method of it simulates and detects coloured soluble component distribution in freeze-thawing process of soil |
CN112945951A (en) * | 2021-03-21 | 2021-06-11 | 西北农林科技大学 | Method for color development determination of iron distribution and content of different parts of wheat grains |
CN113655011A (en) * | 2021-10-20 | 2021-11-16 | 武汉新烽光电股份有限公司 | Microfluidic detection system and detection method thereof |
CN113655011B (en) * | 2021-10-20 | 2022-02-08 | 武汉新烽光电股份有限公司 | Microfluidic detection system and detection method thereof |
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