CN104498490A - Molecular marker tightly linked with low-protein-content QTL of wheat grains and application of molecular marker - Google Patents
Molecular marker tightly linked with low-protein-content QTL of wheat grains and application of molecular marker Download PDFInfo
- Publication number
- CN104498490A CN104498490A CN201410817383.1A CN201410817383A CN104498490A CN 104498490 A CN104498490 A CN 104498490A CN 201410817383 A CN201410817383 A CN 201410817383A CN 104498490 A CN104498490 A CN 104498490A
- Authority
- CN
- China
- Prior art keywords
- wheat
- protein content
- sequence seq
- primer sequence
- peaceful
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a molecular marker tightly linked with low-protein-content QTL of wheat grains and an application of the molecular marker. The molecular marker is characterized in that DNA of a wheat variety Ningmai 9 is subjected to PCR amplification by virtue of an upstream primer sequence SEQ ID NO.1 and a downstream primer sequence SEQ ID NO.2, after an amplified product is subjected to electrophoretic separation on 6% polyacrylamide gel, a molecular marker Xgwm149 which is tightly linked with the low-protein-content QTL of the wheat grains is obtained, and the size of the molecular marker is 175bp; and DNA of the wheat variety Ningmai 9 is subjected to PCR amplification by virtue of an upstream primer sequence SEQ ID NO.3 and a downstream primer sequence SEQ ID NO.4, after the amplified product is subjected to electrophoretic separation on 6% polyacrylamide gel, another molecular marker Xwmc657 which is tightly linked with the low-protein-content QTL of the wheat grains is obtained, and the size of the molecular marker is 119bp. By virtue of the molecular marker, QTL for controlling the protein content of the grains can be selected in a low-generation seedling stage, so that the workload of a breeder for selecting the characters is beneficially reduced, and the selection accuracy and the breeding efficiency are improved.
Description
Technical field
The present invention relates to wheat breeding and biology field, particularly a kind of and the closely linked molecule marker of wheat grain lower protein content QTL and application thereof.
Background technology
Along with improving constantly of China's wheat yield, the demand of people to good quality wheat increases day by day, according to wheat quality, wheat is divided into strong gluten wheat, middle gluten wheat and weak-gluten wheat by China, weak-gluten wheat is mainly for the production of flour products such as biscuit, cake and southern steamed buns, Protein Content of Wheat Kernel is the important indicator of quality, the wheat breed that grain protein content is lower can as the raw material of production high-quality weak-gluten wheat goods, and China's high-quality weak-gluten wheat national standard (GB/T17893-1999) regulation wheat grain crude protein content is no more than 11.5%.
Because grain protein content just must can carry out attribute test after harvesting, and be subject to environmental influence, therefore efficiency of selection is lower, molecular marker assisted selection can be selected breeding material for lower protein content gene or QTL at DNA level, thus can select in seedling stage, and the unstable of grain protein content qualification can be overcome, reduce the cost of phenotypic evaluation, improve weak-gluten wheat quality breeding efficiency.Blanco etc. (2002) have found 7 QTL site controlling grain protein content, lay respectively on 4B, 5A, 6A, 6B, 7A and 7B karyomit(e), Prasad etc. (1999) are positioned at the QTL site controlling grain protein content on 2D karyomit(e), Huang (2006) etc. utilizes double rate, 4B and 4D karyomit(e) finds the QTL controlling grain protein content, Li etc. (2007) utilize two recombinant inbred lines containing 229 and 485 familys 3 and 10 QTL affecting grain protein content to be detected respectively, be positioned 2B respectively, 3A, 4A, 4D, 5B, 7A, 7B and 1A, 1B, 2A, 2D, 3A, 4B, 5A, 5D, 6B, on 7D karyomit(e), with current disclosed documents and materials, the gene or the QTL that control grain protein content are more, effect value is not high, from the gene or QTL of numerous control grain protein contents, how to find suitable site and linkage molecule mark thereof, in order to judge grain protein content, it is this area technical barrier urgently to be resolved hurrily always.
Summary of the invention
For solving an above-mentioned difficult problem, provide a kind of and the closely linked molecule marker of wheat grain lower protein content QTL, in order to detect the grain protein content of the peaceful wheat of wheat breed No. 9, accelerate high-quality weak-gluten wheat and select progress, the present invention is achieved in that
One and the closely linked molecule marker of wheat grain lower protein content QTL, by upstream primer sequence SEQ IDNO.1 and downstream primer sequence SEQ ID NO.2, the DNA to the peaceful wheat of wheat breed No. 9 has carried out pcr amplification, amplified production after electrophoretic separation, obtains the molecule marker Xgwm149 closely linked with wheat grain lower protein content QTL that size is 175bp on 6% polyacrylamide gel; Pcr amplification has been carried out by the DNA of upstream primer sequence SEQ ID NO.3 and downstream primer sequence SEQ IDNO.4 to the peaceful wheat of wheat breed No. 9, amplified production on 6% polyacrylamide gel after electrophoretic separation, obtain size be 119bp with wheat grain lower protein content QTL another molecule marker closely linked Xwmc657.
In the present invention, the DNA of the peaceful wheat of described wheat breed No. 9 refers to the DNA obtained after separation and Extraction in blade to peaceful wheat No. 9 wheat plants, seed tissue;
Described 6% polyacrylamide gel refers in 100ml polyacrylamide sol solution containing 5.7 grams of acrylamides and 0.3 gram of methylene diacrylamide;
The condition of described pcr amplification is:
PCR amplification system is 20 μ l, comprising: the upstream primer of 10 μm of ol/L and downstream primer, each 1.0 μ l, 10 × Buffer 2.0 μ l, the Mg of 25mmol/L
2+the Taq enzyme 0.2 μ l of 1.2 μ l, 5U/ μ l, the DNA2.0 μ l of the dNTP0.6 μ l of 2.5mmol/L, 30ng/ μ l, all the other are supplied with ddH2O;
Pcr amplification program is: 94 DEG C of denaturation 7min; 94 DEG C of sex change 30s, 55/61 DEG C of annealing 45s, 72 DEG C extend 60s, 34 circulations; 7min is extended after 72 DEG C; Last 10 DEG C of termination reactions.
The application of molecule marker described in the present invention, be used for the peaceful wheat of wheat breed No. 9 and derived varieties or strain genotype detection by with closely linked two molecule marker Xgwm149 and Xwmc657 of wheat grain lower protein content QTL, to judge whether this kind or strain seed have low protein content feature.
The application of molecule marker of the present invention, by peaceful for wheat breed wheat No. 9 and derived varieties thereof or product be male parent or maternal with other wheat hybridizings and multiply to F2 for more than, obtain the new variety containing peaceful wheat No. 9 genes or new lines, be used for the new variety containing peaceful wheat No. 9 genes or new lines genotype detection by with closely linked two molecule marker Xgwm149 and Xwmc657 of wheat grain lower protein content QTL, to judge whether these new variety or new lines seed have low protein content feature.
In the application of molecule marker of the present invention, it is separation and Extraction genomic dna from tested wheat plant blade, seed tissue, upstream primer sequence SEQ ID NO.1 and downstream primer sequence SEQ ID NO.2, and upstream primer sequence SEQ IDNO.3 and downstream primer sequence SEQ ID NO.4 carries out pcr amplification to these DNA respectively, whether exist by passing judgment on molecule marker Xgwm149 and Xwmc657, predict whether this kind or strain have the low protein characteristic of seed, if existed simultaneously, grain protein content is " low ", otherwise is " height ";
Described " low " refers to: Protein Content of Wheat Kernel is less than 11.5;
Described " height " refers to: Protein Content of Wheat Kernel is more than or equal to 11.5.
The application of molecule marker of the present invention, is characterized in that, the peaceful wheat of described wheat breed No. 9 and derived varieties thereof or strain refer to, with the peaceful wheat of wheat No. 9 all wheat breeds that to be parent obtained by conventional hybridization or strain.
Peaceful wheat No. 9 is the high-quality weak-gluten wheat kind of the stay in grade that China generally acknowledges, its grain protein content meets the requirement of national high-quality weak-gluten wheat, be configured with recombinant inbred lines with peaceful wheat No. 9 for parent and middle gluten wheat Yangmai No.158 in the present invention, QTL location is carried out to grain protein content, obtain closely linked molecule marker Xgwm149 and Xwmc657, Ordinary fruit quality breeding cycle can be overcome long by this molecular mark, the deficiency that seedling stage is fubaritic, Ordinary fruit quality breeding could can only detect grain protein content after hybridization 4 generation, the QTL controlling grain protein content can be selected in the seedling stage of low generation, be conducive to reducing breeding man to the workload of this character determination, improve the accuracy and breeding efficiency selected, a kind of efficient, new technology of breeding fast.
Accompanying drawing explanation
Fig. 1 is the LOD Distribution value figure of the qtl analysis of peaceful wheat No. 9 seed lower protein contents.
Fig. 2 is the electrophoretic band figure carrying out pcr amplification with the peaceful wheat of wheat line No. 9 F7 that is parent for 12 derivative strain trip primer sequence SEQ IDNO.1 of RIL colony and downstream primer sequence SEQ ID NO.2;
Wherein, M: molecular weight standard; N: peaceful wheat No. 9; Y: Yangmai No.158; 1-12 swimming lane is derivative strain, and they are 19,21 respectively, 35,48,50,64,70,82,101,120,135,148.
Fig. 3 is the electrophoretic band figure carrying out pcr amplification with the peaceful wheat of wheat line No. 9 F7 that is parent for 12 derivative strain trip primer sequence SEQ IDNO.3 of RIL colony and downstream primer sequence SEQ ID NO.4;
M: molecular weight standard; N: peaceful wheat No. 9; Y: Yangmai No.158; 1-12 swimming lane is derivative strain, and swimming lane is derivative strain, and they are 19,21 respectively, 35,48,50,64,70,82,101,120,135,148.
Embodiment
Primer pair designed by embodiment:
Upstream primer sequence SEQ ID NO.1:CATTGTTTTCTGCCTCTAGCC;
Downstream primer sequence SEQ ID NO.2:CTAGCATCGAACCTGAACAAG;
Upstream primer sequence SEQ ID NO.3:CGGGCTGCGGGGGTAT;
Downstream primer sequence SEQ ID NO.4:CGGTTGGGTCATTTGTCTCA;
Embodiment 1: to the mensuration of the peaceful wheat of seed lower protein content wheat breed No. 9
1, the DNA of the peaceful wheat of wheat breed No. 9 is obtained
Adopted by the plant leaf of peaceful for wheat breed wheat No. 9 CTAB extracting method to carry out separation and Extraction and obtain DNA.
2, the peaceful wheat of wheat breed No. 9 DNA are detected, filter out and the closely linked molecule marker of seed lower protein content QTL
Utilize upstream primer sequence SEQ ID NO.1 and downstream primer sequence SEQ ID NO.2 to carry out pcr amplification to the DNA obtained, the condition of amplification is:
PCR amplification system is 20 μ l, comprising: the upstream primer SEQ ID NO.1 of 10 μm of ol/L and downstream primer SEQ IDNO.2, each 1.0 μ l, 10 × Buffer 2.0 μ l, the Mg of 25mmol/L
2+the Taq enzyme 0.2 μ l of 1.2 μ l, 5U/ μ l, the DNA2.0 μ l of the dNTP0.6 μ l of 2.5mmol/L, 30ng/ μ l, all the other are supplied with ddH2O;
Pcr amplification program is: 94 DEG C of denaturation 7min; 94 DEG C of sex change 30s, 55/61 DEG C of annealing 45s, 72 DEG C extend 60s, 34 circulations; 7min is extended after 72 DEG C; Last 10 DEG C of termination reactions.
Amplified production after electrophoretic separation, obtains the molecule marker Xgwm149 that size is 175bp on 6% polyacrylamide gel.
Utilize upstream primer sequence SEQ ID NO.3 and downstream primer sequence SEQ ID NO.4 to carry out pcr amplification to the DNA obtained, the condition of amplification is:
PCR amplification system is 20 μ l, comprising: the upstream primer SEQ ID NO.3 of 10 μm of ol/L and downstream primer SEQ IDNO.4 each 1.0 μ l, 10 × Buffer 2.0 μ l, the Mg of 25mmol/L
2+the Taq enzyme 0.2 μ l of 1.2 μ l, 5U/ μ l, the DNA2.0 μ l of the dNTP0.6 μ l of 2.5mmol/L, 30ng/ μ l, all the other are supplied with ddH2O;
Pcr amplification program is: 94 DEG C of denaturation 7min; 94 DEG C of sex change 30s, 55/61 DEG C of annealing 45s, 72 DEG C extend 60s, 34 circulations; 7min is extended after 72 DEG C; Last 10 DEG C of termination reactions;
Amplified production after electrophoretic separation, obtains the molecule marker Xwmc657 that size is 119bp on 6% polyacrylamide gel.
In the present embodiment, 6% polyacrylamide gel refers to: containing 5.7 grams of acrylamides and 0.3 gram of methylene diacrylamide in 100ml polyacrylamide solution.
Windows QTL Cartographer V2.5 software composite interval mapping method is utilized to detect, obtain the LOD Distribution value figure of the 4B karyomit(e) seed lower protein content QTL of peaceful wheat No. 9, as shown in Figure 1, wherein: Figure 1A is the genetic linkage map of 4B chromosomal region, Figure 1B is the LOD Distribution value figure of QTL, as seen from Figure 1, molecule marker Xgwm149 and Xwmc657 in the both sides of this QTL, with this QTL close linkage.
Embodiment 2 utilizes the derivative strain of molecule marker to the peaceful wheat of wheat breed No. 9 to carry out Prediction of Grain Protein Content
Adopt the peaceful wheat of wheat breed No. 9 and Yangmai No.158 to hybridize, multiply to F7 generation, different strains is defined as 19 respectively, and 21,35,48,50,64,70,82,101,120,135,148;
Adopt CTAB method DNA isolation from these plant leafs respectively, then upstream primer sequence SEQ ID NO.1 and downstream primer sequence SEQ ID NO.2 is used, and upstream primer sequence carries out pcr amplification (PCR amplification method is identical with embodiment 1) to obtained DNA respectively with downstream primer sequence SEQ ID NO.4, amplified production is electrophoretic separation on 6% polyacrylamide gel, pass judgment on obtain in the PCR primer of DNA whether there is the molecule marker Xwmc657 that molecule marker Xgwm149 that size is 175bp and size are 119bp simultaneously, acquired results is as Fig. 2, shown in Fig. 3, Fig. 2 is for the electrophoretic band figure more than 12 derivative strains of RIL colony swimming primer sequence SEQ ID NO.1 and downstream primer sequence SEQ ID NO.2 and carry out pcr amplification with the peaceful wheat of wheat line No. 9 F7 that is parent, wherein, M: molecular weight standard, N: peaceful wheat No. 9, Y: Yangmai No.158, 1-12 swimming lane is derivative strain, and they are 19,21 respectively, 35,48,50,64,70,82,101,120,135,148,
Fig. 3 is for the electrophoretic band figure more than 12 derivative strains of RIL colony swimming primer sequence SEQ IDNO.3 and downstream primer sequence SEQ ID NO.4 and carry out pcr amplification, M: molecular weight standard with the peaceful wheat of wheat line No. 9 F7 that is parent; N: peaceful wheat No. 9; Y: Yangmai No.158; 1-12 swimming lane is derivative strain, and swimming lane is derivative strain, and they are 19,21 respectively, 35,48,50,64,70,82,101,120,135,148.
Judging criterion: if simultaneously above-mentioned two molecule markers, namely occur the specific band of corresponding 175bp or 119bp in electrophorogram, then illustrate that this strain grain protein content reaches " low " level, otherwise be " height ";
Definition Protein Content of Wheat Kernel is less than 11.5 for " low ", is more than or equal to 11.5 for " height ".
Based on this decision method and standard, the grain protein content to each wheat plant is predicted, after harvesting wheat, grain protein content is surveyed and with the comparison that predicts the outcome, predict the outcome with measured result coincide, result is see table 1.
The F7 offspring plant part Prediction of Grain Protein Content of the peaceful wheat No. 9 of table 1 and Yangmai No.158 and practical measurement result
Corresponding specific band is there is in "+" expression electrophoresis;
Corresponding specific band is there is not in "-" expression electrophoresis.
Embodiment 3 utilizes molecule marker to carry out Prediction of Grain Protein Content to the wheat hybridizing offspring being parent with the peaceful wheat of wheat breed No. 9 derived prodss
Adopt No. 35 strains in embodiment 2 to continue to hybridize with Yangmai No.158, multiply to F4 generation, different strains is defined as 1 respectively, and 2,3,4,5,6;
Adopt CTAB method DNA isolation from these plant leafs respectively, then upstream primer sequence SEQ ID NO.1 and downstream primer sequence SEQ ID NO.2 is used, and upstream primer sequence carries out pcr amplification (PCR amplification method is identical with embodiment 1) to obtained DNA respectively with downstream primer sequence SEQ ID NO.4, amplified production is electrophoretic separation on 6% polyacrylamide gel, pass judgment on obtain in the PCR primer of DNA whether there is the molecule marker Xwmc657 that molecule marker Xgwm149 that size is 175bp and size are 119bp simultaneously.
Judging criterion: if simultaneously above-mentioned two molecule markers, namely occur the specific band of corresponding 175bp or 119bp in electrophorogram, then illustrate that this strain grain protein content reaches " low " level, otherwise be " height ";
Definition Protein Content of Wheat Kernel is less than 11.5 for " low ", is more than or equal to 11.5 for " height ".
Based on this decision method and standard, the grain protein content to each wheat plant is predicted, after harvesting wheat, grain protein content is surveyed and with the comparison that predicts the outcome, predict the outcome with measured result coincide, result is see table 2.
The F4 offspring plant part Prediction of Grain Protein Content of the peaceful wheat No. 9/Yangmai No.158 RILF7-21 of table 2 and Yangmai No.158 and practical measurement result
Corresponding specific band is there is in "+" expression electrophoresis;
Corresponding specific band is there is not in "-" expression electrophoresis.
Above-described embodiment is not to concrete restriction of the present invention, in the specific implementation, can utilize and the present invention relates to the derivative strain of molecule marker Xgwm149 and Xwmc657 to the peaceful wheat of wheat breed No. 9 that size is respectively 175bp and 119bp and carry out grain protein content horizontal forecast.The peaceful wheat of described wheat breed No. 9 derived varietiess or strain refer to the peaceful wheat of wheat breed No. 9 wheat breed that to be parent obtained by conventional hybridization or the means such as to backcross or strain.
SEQUENCE LISTING
<110> Jiangsu Province Agriculture Science Institute
<120> and the closely linked molecule marker of wheat grain lower protein content QTL and application thereof
<130> 4
<160> 4
<170> PatentIn version 3.3
<210> 1
<211> 21
<212> DNA
<213> synthetic
<400> 1
cattgttttc tgcctctagc c 21
<210> 2
<211> 21
<212> DNA
<213> synthetic
<400> 2
ctagcatcga acctgaacaa g 21
<210> 3
<211> 16
<212> DNA
<213> synthetic
<400> 3
cgggctgcgg gggtat 16
<210> 4
<211> 20
<212> DNA
<213> synthetic
<400> 4
cggttgggtc atttgtctca 20
Claims (6)
1. one kind and the closely linked molecule marker of wheat grain lower protein content QTL, it is characterized in that, by upstream primer sequence SEQ ID NO.1 and downstream primer sequence SEQ ID NO.2, the DNA to the peaceful wheat of wheat breed No. 9 has carried out pcr amplification, amplified production after electrophoretic separation, obtains the molecule marker closely linked with wheat grain lower protein content QTL that size is 175bp on 6% polyacrylamide gel
xgwm149; By upstream primer sequence SEQ ID NO.3 and downstream primer sequence SEQ ID NO.4, the DNA to the peaceful wheat of wheat breed No. 9 has carried out pcr amplification, amplified production after electrophoretic separation, obtains another molecule marker closely linked with wheat grain lower protein content QTL that size is 119bp on 6% polyacrylamide gel
xwmc657.
2. according to claim 1 with the closely linked molecule marker of wheat grain lower protein content QTL, it is characterized in that, the DNA of the peaceful wheat of described wheat breed No. 9 refers to the DNA obtained after separation and Extraction in blade to peaceful wheat No. 9 wheat plants, seed tissue;
Described 6% polyacrylamide gel refers in 100ml polyacrylamide sol solution containing 5.7 grams of acrylamides and 0.3 gram of methylene diacrylamide.
3. the application of molecule marker as claimed in claim 1, is characterized in that, will two molecule markers closely linked with wheat grain lower protein content QTL
xgwm149with
xwmc657for the genotype detection to the peaceful wheat of wheat breed No. 9 and derived varieties or strain, to judge whether this kind or strain seed have low protein content feature.
4. the application of molecule marker according to claim 3, it is characterized in that: by peaceful for wheat breed wheat No. 9 and derived varieties thereof or product be male parent or maternal with other wheat hybridizings and multiply to F2 for more than, obtain the new variety containing peaceful wheat No. 9 genes or new lines, will two molecule markers closely linked with wheat grain lower protein content QTL
xgwm149with
xwmc657for the genotype detection to the new variety containing peaceful wheat No. 9 genes or new lines, to judge whether these new variety or new lines seed have low protein content feature.
5. the application of the molecule marker according to claim 3 or 4, it is characterized in that: separation and Extraction genomic dna from tested wheat plant blade, seed tissue, upstream primer sequence SEQ ID NO.1 and downstream primer sequence SEQ ID NO.2, and upstream primer sequence SEQ ID NO.3 and downstream primer sequence SEQ ID NO.4 carries out pcr amplification to these DNA respectively, by passing judgment on molecule marker
xgwm149with
xwmc657whether exist, predict whether this kind or strain have the low protein characteristic of seed, if existed simultaneously, grain protein content is " low ", otherwise is " height ";
Described " low " refers to: Protein Content of Wheat Kernel is less than 11.5;
Described " height " refers to: Protein Content of Wheat Kernel is more than or equal to 11.5.
6. the application of the molecule marker according to claim 3 or 4, is characterized in that, the peaceful wheat of described wheat breed No. 9 and derived varieties thereof or strain refer to, with the peaceful wheat of wheat No. 9 all wheat breeds that to be parent obtained by conventional hybridization or strain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410817383.1A CN104498490A (en) | 2014-12-24 | 2014-12-24 | Molecular marker tightly linked with low-protein-content QTL of wheat grains and application of molecular marker |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410817383.1A CN104498490A (en) | 2014-12-24 | 2014-12-24 | Molecular marker tightly linked with low-protein-content QTL of wheat grains and application of molecular marker |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104498490A true CN104498490A (en) | 2015-04-08 |
Family
ID=52939942
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410817383.1A Pending CN104498490A (en) | 2014-12-24 | 2014-12-24 | Molecular marker tightly linked with low-protein-content QTL of wheat grains and application of molecular marker |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104498490A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104911272A (en) * | 2015-06-26 | 2015-09-16 | 保定学院 | Primer for screening wheat leaf rust resistance QTL and application of primer |
| CN105802966A (en) * | 2016-05-10 | 2016-07-27 | 中国农业大学 | Wheat NAM gene molecular maker and application thereof |
| CN105821052A (en) * | 2016-05-10 | 2016-08-03 | 中国农业大学 | Aegilops speltoides Tausch NAM-S1 gene and molecular marker and application thereof |
| CN106282395A (en) * | 2016-10-26 | 2017-01-04 | 中国科学院成都生物研究所 | A kind of detect the primer of Semen Tritici aestivi height grain protein content, test kit, detection method and application thereof |
| CN106434950A (en) * | 2016-10-26 | 2017-02-22 | 中国科学院成都生物研究所 | Primer, kit and method for detecting low grain protein content of wheat, and applications of method |
| CN108570517A (en) * | 2018-06-12 | 2018-09-25 | 江苏省农业科学院 | The relevant specific primer of low with the peaceful wheat of weak-gluten wheat No. 9 albumen and its application |
| CN110373489A (en) * | 2019-07-10 | 2019-10-25 | 江苏省农业科学院 | KASP label relevant to wheat seed protein content and its application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101760552A (en) * | 2008-12-19 | 2010-06-30 | 李祥 | Molecular mark research evolution of main dwarf genes |
| CN102626048A (en) * | 2012-05-02 | 2012-08-08 | 南京农业大学 | Method of molecular marker assisted backcross to improve scab invasion resistance of wheat |
-
2014
- 2014-12-24 CN CN201410817383.1A patent/CN104498490A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101760552A (en) * | 2008-12-19 | 2010-06-30 | 李祥 | Molecular mark research evolution of main dwarf genes |
| CN102626048A (en) * | 2012-05-02 | 2012-08-08 | 南京农业大学 | Method of molecular marker assisted backcross to improve scab invasion resistance of wheat |
Non-Patent Citations (4)
| Title |
|---|
| DARYL J. SOMERS ET AL.: "A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.)", 《THEOR APPL GENET》 * |
| MARION S. RöDER ET AL.: "A Microsatellite Map of Wheat", 《GENETICS》 * |
| 姚金保 等: "软质冬小麦品种籽粒蛋白质含量的遗传分析", 《江苏农业学报》 * |
| 常斌: "弱筋小麦宁麦9号品质性状QTL定位", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104911272A (en) * | 2015-06-26 | 2015-09-16 | 保定学院 | Primer for screening wheat leaf rust resistance QTL and application of primer |
| CN104911272B (en) * | 2015-06-26 | 2017-10-03 | 保定学院 | A kind of primer and its application for being used to screen wheat leaf rust resistance QTL |
| CN105802966B (en) * | 2016-05-10 | 2019-01-25 | 中国农业大学 | Wheat NAM gene molecule marker and its application |
| CN105802966A (en) * | 2016-05-10 | 2016-07-27 | 中国农业大学 | Wheat NAM gene molecular maker and application thereof |
| CN105821052A (en) * | 2016-05-10 | 2016-08-03 | 中国农业大学 | Aegilops speltoides Tausch NAM-S1 gene and molecular marker and application thereof |
| CN105821052B (en) * | 2016-05-10 | 2019-05-10 | 中国农业大学 | Ae.speltoides NAM-S1 gene and its molecular labeling and application |
| CN106434950B (en) * | 2016-10-26 | 2019-07-05 | 中国科学院成都生物研究所 | A kind of primer, kit, detection method and its application detecting the low grain protein content of wheat |
| CN106434950A (en) * | 2016-10-26 | 2017-02-22 | 中国科学院成都生物研究所 | Primer, kit and method for detecting low grain protein content of wheat, and applications of method |
| CN106282395A (en) * | 2016-10-26 | 2017-01-04 | 中国科学院成都生物研究所 | A kind of detect the primer of Semen Tritici aestivi height grain protein content, test kit, detection method and application thereof |
| CN106282395B (en) * | 2016-10-26 | 2019-08-20 | 中国科学院成都生物研究所 | A kind of primer, kit, detection method and its application detecting the high grain protein content of wheat |
| CN108570517A (en) * | 2018-06-12 | 2018-09-25 | 江苏省农业科学院 | The relevant specific primer of low with the peaceful wheat of weak-gluten wheat No. 9 albumen and its application |
| CN108570517B (en) * | 2018-06-12 | 2021-10-01 | 江苏省农业科学院 | Specific primer related to Ning-Mai No. 9 low protein of weak gluten wheat and application of specific primer |
| CN110373489A (en) * | 2019-07-10 | 2019-10-25 | 江苏省农业科学院 | KASP label relevant to wheat seed protein content and its application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104498490A (en) | Molecular marker tightly linked with low-protein-content QTL of wheat grains and application of molecular marker | |
| CN102162011B (en) | Molecule marking method of rice blast-resisting gene | |
| CN103146695B (en) | Functional molecular marker for rice anti-blast gene Pi9 and application thereof | |
| CN102154470B (en) | Molecular marker method for anti-rice blast gene Pi-hk1 (t) of paddy | |
| CN103820476A (en) | Gene relevant to wheat thousand seed weight, functional marker and application thereof | |
| US20200015444A1 (en) | Methods of creating drought tolerant corn plants using markers linked to cold shock domain-containing proteins and compositions thereof | |
| CN106555001B (en) | A kind of molecular labeling of rice blast resistant gene and its application | |
| CN105506149A (en) | Linkage SNP locus and CAPS marker of watermelon fruit sugar accumulation gene STP1 | |
| CN112795691B (en) | Molecular marker linked with corn stem thickness and application thereof | |
| CN105219858B (en) | Grain Weight in Common Wheat gene TaGS5 3A single nucleotide polymorphisms and its application | |
| CN104152450B (en) | The InDel molecular labelings isolated with cucumber powdery mildew resistance main effect gene | |
| CN105087573A (en) | Method for identifying rice Wx-mw gene and application of rice Wx-mw gene in high-quality rice breeding | |
| CN105463118A (en) | Gene TaGDRG-2A molecular marker related to wheat plant type and yield and application thereof | |
| CN103789419B (en) | Co-dominance tag primer group for identifying allele type of rice photo-thermo-sensitive genic male-sterile gene p/tms12-1, and applications thereof | |
| CN103409418A (en) | Molecular markers in close linkage with large grain gene GS2 of rice and application thereof | |
| CN104789648A (en) | Molecular markers for haplotype identification of paddy rice CMS restoring gene Rf-1 segment and applications thereof | |
| CN106755465A (en) | The molecular labeling of QTL QFll.sicau 2D close linkage long with wheat flag leaf and application | |
| CN105713983B (en) | A kind of molecular labeling and its application with rice Jiangnan evening neck blast resistance gene close linkage | |
| CN104073488A (en) | Molecular marker for dwarfing wheat marker gene, and primer and application thereof | |
| CN103834647A (en) | SSR marker Xgwm537 in close linkage with wheat dwarf gene RhtDC20 and application thereof | |
| CN107746895A (en) | It is a kind of it is low-phosphorous under the conditions of lifted barley harvest index QTL site molecular labeling and application | |
| CN106834527B (en) | Molecular marker closely linked with wheat seedling sheath blight resistance QTL and application thereof | |
| CN102071195B (en) | Molecular marker for content of sclereid in pyrus communis fruits | |
| CN106636395B (en) | Functional molecular marker Pi2-1 of rice blast-resistant gene Pi2 and application thereof | |
| CN105524994A (en) | Molecular marker HRM7 of barley grain length gene LkI2 and application of molecular marker |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150408 |